HO063294

EST Overview


Name	HO063294
Unique Name	HO063294
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	302

Libraries


Library Name	Type
LIBEST_026410 Chickpea drought stressed cDNA SSH library AR1	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO063294 vs. SwissProt
Match: GIT7_SCHPO (Glucose-insensitive transcription protein 7 OS=Schizosaccharomyces pombe GN=git7 PE=2 SV=2)

HSP 1 Score: 72.0182 bits (175), Expect = 1.062e-12
Identity = 32/53 (60.38%), Postives = 41/53 (77.36%), Query Frame = -3

Query:  136 YAGIYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKW 294
            +  +Y+NAD+D RRAM KS+ ESNGT LST+WK+V SK  E  PP+GME KK+
Sbjct:  327 FQNLYKNADDDTRRAMMKSYTESNGTALSTNWKDVKSKTFETKPPQGMEPKKF 379

[Close]

BLAST of HO063294 vs. SwissProt
Match: SUGT1_DICDI (Protein SUGT1 homolog OS=Dictyostelium discoideum GN=sugt1 PE=2 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 2.366e-12
Identity = 32/54 (59.26%), Postives = 40/54 (74.07%), Query Frame = -3

Query:  133 YAGIYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWE 294
            +  I+    ED +RAM KSF ES GTVLST+W EVGSKKV G PP+G+E K++E
Sbjct:  333 FRDIFSKGSEDQQRAMMKSFTESGGTVLSTNWDEVGSKKVVGEPPKGLEFKQYE 386

[Close]

BLAST of HO063294 vs. SwissProt
Match: SGT1_YEAST (Protein SGT1 OS=Saccharomyces cerevisiae GN=SGT1 PE=1 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 5.270e-12
Identity = 31/50 (62.00%), Postives = 37/50 (74.00%), Query Frame = -3

Query:  136 IYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKW 285
            +Y  AD D +RAM KSF+ESNGT LSTDW++V    V+ SPPEGME K W
Sbjct:  346 LYAGADPDTKRAMMKSFIESNGTALSTDWEDVSKGTVKTSPPEGMEPKHW 395

[Close]

BLAST of HO063294 vs. SwissProt
Match: SUGT1_HUMAN (Suppressor of G2 allele of SKP1 homolog OS=Homo sapiens GN=SUGT1 PE=1 SV=3)

HSP 1 Score: 65.855 bits (159), Expect = 7.610e-11
Identity = 28/50 (56.00%), Postives = 41/50 (82.00%), Query Frame = -3

Query:  136 IYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKW 285
            IY +  ++++RAM+KSF+ES GTVLST+W +VG +KVE +PP+ ME KK+
Sbjct:  316 IYSDGSDEVKRAMNKSFMESGGTVLSTNWSDVGKRKVEINPPDDMEWKKY 365

[Close]

BLAST of HO063294 vs. SwissProt
Match: SUGT1_BOVIN (Suppressor of G2 allele of SKP1 homolog OS=Bos taurus GN=SUGT1 PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 7.610e-11
Identity = 28/50 (56.00%), Postives = 41/50 (82.00%), Query Frame = -3

Query:  136 IYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKW 285
            IY +  ++++RAM+KSF+ES GTVLST+W +VG +KVE +PP+ ME KK+
Sbjct:  289 IYSDGSDEVKRAMNKSFMESGGTVLSTNWSDVGKRKVEINPPDDMEWKKY 338

[Close]

BLAST of HO063294 vs. SwissProt
Match: SUGT1_RAT (Suppressor of G2 allele of SKP1 homolog OS=Rattus norvegicus GN=Sugt1 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.214e-10
Identity = 27/50 (54.00%), Postives = 41/50 (82.00%), Query Frame = -3

Query:  136 IYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKW 285
            IY +  ++++RAM+KSF+ES GTVLST+W +VG +KVE +PP+ ME K++
Sbjct:  287 IYSDGSDEVKRAMNKSFMESGGTVLSTNWSDVGKRKVEINPPDDMEWKQY 336

[Close]

BLAST of HO063294 vs. SwissProt
Match: SUGT1_MOUSE (Suppressor of G2 allele of SKP1 homolog OS=Mus musculus GN=Sugt1 PE=2 SV=3)

HSP 1 Score: 64.3142 bits (155), Expect = 2.214e-10
Identity = 27/50 (54.00%), Postives = 41/50 (82.00%), Query Frame = -3

Query:  136 IYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKW 285
            IY +  ++++RAM+KSF+ES GTVLST+W +VG +KVE +PP+ ME K++
Sbjct:  287 IYSDGSDEVKRAMNKSFMESGGTVLSTNWSDVGKRKVEINPPDDMEWKQY 336

[Close]

BLAST of HO063294 vs. TrEMBL
Match: B6EBD4_SOYBN (SGT1-1 OS=Glycine max PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 9.510e-23
Identity = 51/55 (92.73%), Postives = 53/55 (96.36%), Query Frame = -3

Query:  130 YAGIYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWEY 294
            +  IYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGME+KKWEY
Sbjct:  306 FRDIYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMELKKWEY 360

[Close]

BLAST of HO063294 vs. TrEMBL
Match: C6TJ65_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 2.119e-22
Identity = 50/55 (90.91%), Postives = 53/55 (96.36%), Query Frame = -3

Query:  130 YAGIYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWEY 294
            +  IYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGME+KKW+Y
Sbjct:  307 FRDIYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMELKKWQY 361

[Close]

BLAST of HO063294 vs. TrEMBL
Match: B7FIH5_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 2.119e-22
Identity = 51/55 (92.73%), Postives = 52/55 (94.55%), Query Frame = -3

Query:  130 YAGIYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWEY 294
            +  IYQNADEDMR AMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWEY
Sbjct:  175 FRDIYQNADEDMRSAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWEY 229

[Close]

BLAST of HO063294 vs. TrEMBL
Match: B9RCI2_RICCO (Chaperone binding protein, putative OS=Ricinus communis GN=RCOM_1689140 PE=4 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 5.769e-20
Identity = 46/55 (83.64%), Postives = 51/55 (92.73%), Query Frame = -3

Query:  130 YAGIYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWEY 294
            +  IYQNADEDMRRAM KSF+ES+GTVLSTDWKEVGSKKVEGS PEGME++KWEY
Sbjct:  208 FRDIYQNADEDMRRAMMKSFVESSGTVLSTDWKEVGSKKVEGSAPEGMEMRKWEY 262

[Close]

BLAST of HO063294 vs. TrEMBL
Match: C5XEC3_SORBI (Putative uncharacterized protein Sb03g028430 OS=Sorghum bicolor GN=Sb03g028430 PE=4 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.679e-19
Identity = 44/55 (80.00%), Postives = 52/55 (94.55%), Query Frame = -3

Query:  130 YAGIYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWEY 294
            +  IY++ADEDMRRAM KSF+ESNGTVLST+WK+VGSKKVEGSPP+GME+KKWEY
Sbjct:  310 FRDIYKDADEDMRRAMMKSFVESNGTVLSTNWKDVGSKKVEGSPPDGMELKKWEY 364

[Close]

BLAST of HO063294 vs. TrEMBL
Match: Q9SE32_ORYSA (Sgt1 OS=Oryza sativa GN=Sgt1 PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.192e-19
Identity = 44/55 (80.00%), Postives = 51/55 (92.73%), Query Frame = -3

Query:  130 YAGIYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWEY 294
            +  IY +ADEDMRRAM KSF+ESNGTVLST+WK+VGSKKVEGSPP+GME+KKWEY
Sbjct:  313 FRDIYSDADEDMRRAMMKSFVESNGTVLSTNWKDVGSKKVEGSPPDGMELKKWEY 367

[Close]

BLAST of HO063294 vs. TrEMBL
Match: Q0JL44_ORYSJ (Os01g0624500 protein OS=Oryza sativa subsp. japonica GN=Os01g0624500 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.192e-19
Identity = 44/55 (80.00%), Postives = 51/55 (92.73%), Query Frame = -3

Query:  130 YAGIYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWEY 294
            +  IY +ADEDMRRAM KSF+ESNGTVLST+WK+VGSKKVEGSPP+GME+KKWEY
Sbjct:  313 FRDIYSDADEDMRRAMMKSFVESNGTVLSTNWKDVGSKKVEGSPPDGMELKKWEY 367

[Close]

BLAST of HO063294 vs. TrEMBL
Match: B9RIR3_RICCO (Chaperone binding protein, putative OS=Ricinus communis GN=RCOM_1581910 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.192e-19
Identity = 44/55 (80.00%), Postives = 51/55 (92.73%), Query Frame = -3

Query:  130 YAGIYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWEY 294
            +  IYQ+ADED RRAM KSF+ESNGTVLST+WKEVGSKKVEGSPP+GME++KWEY
Sbjct:  307 FRDIYQDADEDTRRAMKKSFVESNGTVLSTNWKEVGSKKVEGSPPDGMEMRKWEY 361

[Close]

BLAST of HO063294 vs. TrEMBL
Match: B8ABY6_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_02921 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.192e-19
Identity = 44/55 (80.00%), Postives = 51/55 (92.73%), Query Frame = -3

Query:  130 YAGIYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWEY 294
            +  IY +ADEDMRRAM KSF+ESNGTVLST+WK+VGSKKVEGSPP+GME+KKWEY
Sbjct:  313 FRDIYSDADEDMRRAMMKSFVESNGTVLSTNWKDVGSKKVEGSPPDGMELKKWEY 367

[Close]

BLAST of HO063294 vs. TrEMBL
Match: A6N0C5_ORYSI (Suppressor of g2 allele of skp1 (Fragment) OS=Oryza sativa subsp. indica PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.192e-19
Identity = 44/55 (80.00%), Postives = 51/55 (92.73%), Query Frame = -3

Query:  130 YAGIYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWEY 294
            +  IY +ADEDMRRAM KSF+ESNGTVLST+WK+VGSKKVEGSPP+GME+KKWEY
Sbjct:   74 FRDIYSDADEDMRRAMMKSFVESNGTVLSTNWKDVGSKKVEGSPPDGMELKKWEY 128

[Close]

BLAST of HO063294 vs. TAIR peptide
Match: AT4G11260.1 (| Symbols: ATSGT1B, ETA3, RPR1, EDM1, SGT1B | phosphatase-related | chr4:6851515-6853719 REVERSE LENGTH=358)

HSP 1 Score: 95.9005 bits (237), Expect = 5.412e-21
Identity = 42/55 (76.36%), Postives = 51/55 (92.73%), Query Frame = -3

Query:  130 YAGIYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWEY 294
            ++ IY +ADEDMRRAM+KSF ESNGTVLST+WKEVG+KKVE +PP+GME+KKWEY
Sbjct:  304 FSDIYSSADEDMRRAMNKSFAESNGTVLSTNWKEVGTKKVESTPPDGMELKKWEY 358

[Close]

BLAST of HO063294 vs. TAIR peptide
Match: AT4G23570.3 (| Symbols: SGT1A | phosphatase-related | chr4:12300015-12302493 FORWARD LENGTH=351)

HSP 1 Score: 93.5893 bits (231), Expect = 2.686e-20
Identity = 41/51 (80.39%), Postives = 49/51 (96.08%), Query Frame = -3

Query:  133 IYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWE 285
            IYQNADEDMRRAMSKSF+ESNGTVLST+W+EVG+K +E +PP+GME+KKWE
Sbjct:  300 IYQNADEDMRRAMSKSFVESNGTVLSTNWQEVGTKTIESTPPDGMELKKWE 350

[Close]

BLAST of HO063294 vs. TAIR peptide
Match: AT4G23570.2 (| Symbols: SGT1A | phosphatase-related | chr4:12300015-12302493 FORWARD LENGTH=350)

HSP 1 Score: 93.5893 bits (231), Expect = 2.686e-20
Identity = 41/51 (80.39%), Postives = 49/51 (96.08%), Query Frame = -3

Query:  133 IYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWE 285
            IYQNADEDMRRAMSKSF+ESNGTVLST+W+EVG+K +E +PP+GME+KKWE
Sbjct:  299 IYQNADEDMRRAMSKSFVESNGTVLSTNWQEVGTKTIESTPPDGMELKKWE 349

[Close]

BLAST of HO063294 vs. TAIR peptide
Match: AT4G23570.1 (| Symbols: SGT1A | phosphatase-related | chr4:12300015-12302493 FORWARD LENGTH=350)

HSP 1 Score: 93.5893 bits (231), Expect = 2.686e-20
Identity = 41/51 (80.39%), Postives = 49/51 (96.08%), Query Frame = -3

Query:  133 IYQNADEDMRRAMSKSFLESNGTVLSTDWKEVGSKKVEGSPPEGMEVKKWE 285
            IYQNADEDMRRAMSKSF+ESNGTVLST+W+EVG+K +E +PP+GME+KKWE
Sbjct:  299 IYQNADEDMRRAMSKSFVESNGTVLSTNWQEVGTKTIESTPPDGMELKKWE 349

The following BLAST results are available for this feature:

BLAST of HO063294 vs. SwissProt
BLAST of HO063294 vs. TrEMBL
BLAST of HO063294 vs. TAIR peptide

BLAST of HO063294 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 7


Match Name	E-value	Identity	Description
GIT7_SCHPO	1.062e-12	60.38	Glucose-insensitive transcription protein 7 OS=Sch...	[more]
SUGT1_DICDI	2.366e-12	59.26	Protein SUGT1 homolog OS=Dictyostelium discoideum ...	[more]
SGT1_YEAST	5.270e-12	62.00	Protein SGT1 OS=Saccharomyces cerevisiae GN=SGT1 P...	[more]
SUGT1_HUMAN	7.610e-11	56.00	Suppressor of G2 allele of SKP1 homolog OS=Homo sa...	[more]
SUGT1_BOVIN	7.610e-11	56.00	Suppressor of G2 allele of SKP1 homolog OS=Bos tau...	[more]
SUGT1_RAT	2.214e-10	54.00	Suppressor of G2 allele of SKP1 homolog OS=Rattus ...	[more]
SUGT1_MOUSE	2.214e-10	54.00	Suppressor of G2 allele of SKP1 homolog OS=Mus mus...	[more]

BLAST of HO063294 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B6EBD4_SOYBN	9.510e-23	92.73	SGT1-1 OS=Glycine max PE=2 SV=1	[more]
C6TJ65_SOYBN	2.119e-22	90.91	Putative uncharacterized protein OS=Glycine max PE...	[more]
B7FIH5_MEDTR	2.119e-22	92.73	Putative uncharacterized protein OS=Medicago trunc...	[more]
B9RCI2_RICCO	5.769e-20	83.64	Chaperone binding protein, putative OS=Ricinus com...	[more]
C5XEC3_SORBI	1.679e-19	80.00	Putative uncharacterized protein Sb03g028430 OS=So...	[more]
Q9SE32_ORYSA	2.192e-19	80.00	Sgt1 OS=Oryza sativa GN=Sgt1 PE=2 SV=1	[more]
Q0JL44_ORYSJ	2.192e-19	80.00	Os01g0624500 protein OS=Oryza sativa subsp. japoni...	[more]
B9RIR3_RICCO	2.192e-19	80.00	Chaperone binding protein, putative OS=Ricinus com...	[more]
B8ABY6_ORYSI	2.192e-19	80.00	Putative uncharacterized protein OS=Oryza sativa s...	[more]
A6N0C5_ORYSI	2.192e-19	80.00	Suppressor of g2 allele of skp1 (Fragment) OS=Oryz...	[more]

BLAST of HO063294 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT4G11260.1	5.412e-21	76.36	\| Symbols: ATSGT1B, ETA3, RPR1, EDM1, SGT1B \| phos...	[more]
AT4G23570.3	2.686e-20	80.39	\| Symbols: SGT1A \| phosphatase-related \| chr4:1230...	[more]
AT4G23570.2	2.686e-20	80.39	\| Symbols: SGT1A \| phosphatase-related \| chr4:1230...	[more]
AT4G23570.1	2.686e-20	80.39	\| Symbols: SGT1A \| phosphatase-related \| chr4:1230...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007699	SGS	PFAM	PF05002	SGS	coord: 6..55 score: 5.9
IPR007699	SGS	PROFILE	PS51048	SGS	coord: 1..57 score: 25
None	No IPR available	PANTHER	PTHR22904	TPR REPEAT CONTAINING PROTEIN	coord: 6..53 score: 1.1
None	No IPR available	PANTHER	PTHR22904:SF10	SUPPRESSOR OF G2 ALLELE OF SKP1 (SGT1)	coord: 6..53 score: 1.1

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO063294 ID=HO063294; Name=HO063294; organism=Cicer arietinum; type=EST; length=302bp
GGCGGCCGCGGGAATTCGATTAGCGTGGTCGCGGCCGAGGTACCTAATGA
CAATTTTGGAGGGGATTGGGAAGGAGCAGACAAATGGCTATGAAATAACA
GAAGTTAAATTCATCTTAAAACTGAATCAATACTCCCATTTCTTCACCTC
CATGCCTTCTGGAGGACTGCCTTCCACCTTCTTTGATCCCACTTCTTTCC
AATCAGTTGATAGCACTGTTCCATTTGACTCCAGAAATGACTTGCTCATC
GCTCTCCTCATGTCCTCATCTGCATTTTGGTAGATACCCGCGTACCTGCC
CG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR007699	SGS_dom

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