HO064581

EST Overview
NameHO064581
Unique NameHO064581
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length705
Libraries
Library NameType
LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2cdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Homology
[Close]
BLAST of HO064581 vs. SwissProt
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 206.068 bits (523), Expect = 1.554e-52
Identity = 113/177 (63.84%), Postives = 130/177 (73.45%), Query Frame = -2 
Query:  186 DTHVRTRSFG*DESD-PSKTYLP*KMSSYETLRVVYSLAPLFITTLNFR*LHYWED------IKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK 695
            DTHV       DESD P  TYL  K    ETLR+ Y+ APL +        H   D       KVP+DTIVLINAWAIHRDP+ W+EATTFKPERF+KKGE+EK+IAFGMGRR CPGE LA+R ISMTLALLIQCFDWK  + D ID+AERDGF LTKL+PLKAMCK+RPV+N++ K
Sbjct:  333 DTHVGQDRLV-DESDLPKLTYL--KNVINETLRL-YTPAPLLLP-------HSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLVPLKAMCKSRPVINKVFK 498
[Close]
BLAST of HO064581 vs. SwissProt
Match: C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 2.266e-32
Identity = 62/121 (51.24%), Postives = 85/121 (70.25%), Query Frame = -2 
Query:  189 EDIKV-----PRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRII 536
            ED K+     PR T +L+NAWAIHRDP +W +  +FKPERF+K+ E +K++AFG+GRR CPG  LA R + + L  LIQCF+W+R  +  +DM E  G  + K IPLKA+CK RP +++II
Sbjct:  381 EDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLHKII 501

HSP 2 Score: 22.7126 bits (47), Expect = 2.266e-32
Identity = 8/13 (61.54%), Postives = 10/13 (76.92%), Query Frame = -3 
Query:  536 LLPHSTSDDCIIG 574
            L+PH  S+DC IG
Sbjct:  374 LVPHMASEDCKIG 386
[Close]
BLAST of HO064581 vs. SwissProt
Match: C81F1_ARATH (Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 119.783 bits (299), Expect = 1.465e-26
Identity = 53/116 (45.69%), Postives = 79/116 (68.10%), Query Frame = -2 
Query:  189 VPRDTIVLINAWAIHRDPKSWSEATTFKPERF-DKKGE---IEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRII 524
            VPRDTIV++NAWAIHRDP+ W E   F P+R+ D  G    + K++ FG GRR CPG  L  R +++ L  LIQCF+W+    + +DM+E  G  + K+ PL+AMC+ RP++++++
Sbjct:  384 VPRDTIVMVNAWAIHRDPEIWEEPEKFNPDRYNDGCGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWENVKGEEMDMSESTGLGMRKMDPLRAMCRPRPIMSKLL 499
[Close]
BLAST of HO064581 vs. SwissProt
Match: C71A8_MENPI (Cytochrome P450 71A8 OS=Mentha piperita GN=CYP71A8 PE=3 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 5.046e-19
Identity = 51/107 (47.66%), Postives = 67/107 (62.62%), Query Frame = -2 
Query:  222 VPRDTIVLINAWAIHRDPKSWSEATTFKPERF-----DKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSD-DMIDMAERDGFVLTKLIPLKAM 524
            VP  T+V+INAWAI RDP SW E   F+PERF     D KG   ++I FG GRR CPG    + T+  TLA L+Q FDW+   +   +DM+ER G  + ++IPL A+
Sbjct:  392 VPVGTMVMINAWAIGRDPTSWDEPEKFRPERFLNSSVDFKGLDFELIPFGAGRRGCPGTTFPMATLEFTLANLMQKFDWELPHECRELDMSERPGVAIRRVIPLLAI 498
[Close]
BLAST of HO064581 vs. SwissProt
Match: C76C2_ARATH (Cytochrome P450 76C2 OS=Arabidopsis thaliana GN=CYP76C2 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 2.504e-18
Identity = 48/109 (44.04%), Postives = 64/109 (58.72%), Query Frame = -2 
Query:  222 VPRDTIVLINAWAIHRDPKSWSEATTFKPERF-----DKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWK---RTSDDMIDMAERDGFVLTKLIPLKAM 524
            VP+DT V +N WAI RDP  W  ++ FKPERF     D +G   ++  FG GRR+CPG  LA++T+ + LA L+  FDWK       + +DM E  G  L K  PL A+
Sbjct:  396 VPKDTQVFVNVWAIGRDPNVWENSSRFKPERFLGKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSEDLDMDETFGLTLHKTNPLHAV 504
[Close]
BLAST of HO064581 vs. SwissProt
Match: C71BO_ARATH (Cytochrome P450 71B24 OS=Arabidopsis thaliana GN=CYP71B24 PE=2 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 3.271e-18
Identity = 57/152 (37.50%), Postives = 81/152 (53.29%), Query Frame = -2 
Query:  228 DESDPSKT-YLP*KMSSYETLRVVYSLAPLFITTLNFR*LHYWEDIKVPRDTIVLINAWAIHRDPKSWSEATTFKPERF-----DKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDM-IDMAERDGFVLTKLIPLK 662
            +E D  K  YL  K+   ETLR+ +  APL +       +   +   +P  TI+L+N W+I RDPK W     F PERF     D KG   +++ FG GRR+CPG A A+ T+ + L  L+  FDW+   +D  +DM E     + K +PLK
Sbjct:  344 EEDDVDKLQYL--KLVVKETLRL-HPAAPLLLPRETMSQIKI-QGYNIPSKTILLVNVWSIGRDPKHWKNPEEFNPERFIDCPIDYKGNSFEMLPFGSGRRICPGIAFAIATVELGLLNLLYHFDWRLPEEDKDLDMEEAGDVTIIKKVPLK 491
[Close]
BLAST of HO064581 vs. SwissProt
Match: C76B1_HELTU (7-ethoxycoumarin O-deethylase OS=Helianthus tuberosus GN=CYP76B1 PE=1 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 5.579e-18
Identity = 45/106 (42.45%), Postives = 64/106 (60.38%), Query Frame = -2 
Query:  231 VPRDTIVLINAWAIHRDPKSWSEATTFKPERF-----DKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWK---RTSDDMIDMAERDGFVLTKLIPL 524
            +P  T+V +NAWAI RDP  W ++  FKP+RF     D +G    +I FG GRR+CPG  LA R + + L  L+  FDWK   +   D++DM E++G  ++K  PL
Sbjct:  377 IPAGTLVFVNAWAIGRDPTVWDDSLEFKPQRFLESRLDVRGHDFDLIPFGAGRRICPGIPLATRMVPIMLGSLLNNFDWKIDTKVPYDVLDMTEKNGTTISKAKPL 482
[Close]
BLAST of HO064581 vs. SwissProt
Match: C76C4_ARATH (Cytochrome P450 76C4 OS=Arabidopsis thaliana GN=CYP76C4 PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 7.286e-18
Identity = 49/117 (41.88%), Postives = 68/117 (58.12%), Query Frame = -2 
Query:  198 VPRDTIVLINAWAIHRDPKSWSEATTFKPERF-----DKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTS---DDMIDMAERDGFVLTKLIPLKAMCKTRPVVN 524
            VP+DT VL+N WAI RDP  W   + F+PERF     D KG   ++  FG GRR+CPG  LA++T+S+ LA L+  FDWK  +    + +DM E  G  L +   L A+   +  +N
Sbjct:  395 VPKDTQVLVNVWAIGRDPSVWENPSQFEPERFMGKDIDVKGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETFGITLHRTNTLYAIPVKKQTIN 511
[Close]
BLAST of HO064581 vs. SwissProt
Match: C93A2_SOYBN (Cytochrome P450 93A2 OS=Glycine max GN=CYP93A2 PE=2 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 9.516e-18
Identity = 46/119 (38.66%), Postives = 67/119 (56.30%), Query Frame = -2 
Query:  198 KVPRDTIVLINAWAIHRDPKSWSEATTFKPERF--------DKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWK-RTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVN 527
            ++P  T + +N WAI RDP  W     F+PERF        D +G+    I FG GRR CPG +LAL+ + + LA++IQCF WK    ++ +DM E+ G  L +  P+  +C   P +N
Sbjct:  381 EIPAKTRLFVNVWAIGRDPNHWENPFEFRPERFFENGQSQLDVRGQHYHFIPFGSGRRSCPGTSLALQIVHVNLAIMIQCFQWKFDNGNNKVDMEEKSGITLPRAHPI--ICVPVPRLN 497
[Close]
BLAST of HO064581 vs. SwissProt
Match: C76C1_ARATH (Cytochrome P450 76C1 OS=Arabidopsis thaliana GN=CYP76C1 PE=2 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 9.516e-18
Identity = 51/118 (43.22%), Postives = 68/118 (57.63%), Query Frame = -2 
Query:  198 VPRDTIVLINAWAIHRDPKSWSEATTFKPERF-----DKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTS---DDMIDMAERDGFVLTKLIPLKAM-CKTRPVVN 524
            V +DT VL+N WAI RDP  W   + F+PERF     D +G   ++  FG GRR+CPG  LA++T+S+ LA L+  FDWK       + +DM E  G  L K  PL A+  K R  +N
Sbjct:  395 VLKDTQVLVNVWAIGRDPSVWDNPSQFEPERFLGKDMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWKLPKGVLSEDLDMDETFGLTLHKTNPLHAVPVKKRANIN 512
[Close]
BLAST of HO064581 vs. TrEMBL
Match: Q9XFX0_CICAR (Cytochrome P450 monooxygenase OS=Cicer arietinum GN=cyp81E3v2 PE=2 SV=1)

HSP 1 Score: 238.424 bits (607), Expect = 4.163e-61
Identity = 134/177 (75.71%), Postives = 139/177 (78.53%), Query Frame = -2 
Query:  186 DTHVRTRSFG*DESD-PSKTYLP*KMSSYETLRVVYSLAPLFITTLNFR*LHYWED------IKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK 695
            DTHV       DESD P  TYL  K   YETLR+  + APL +        H   D       KVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKL+PLKAMCKTRPVVN+I K
Sbjct:  333 DTHVGQDRLV-DESDLPKLTYL--KNVIYETLRLC-TPAPLLLP-------HSTSDDCIIGGYKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLVPLKAMCKTRPVVNKIFK 498
[Close]
BLAST of HO064581 vs. TrEMBL
Match: Q9ZRW6_CICAR (Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2 SV=1)

HSP 1 Score: 233.417 bits (594), Expect = 1.339e-59
Identity = 127/166 (76.51%), Postives = 134/166 (80.72%), Query Frame = -2 
Query:  186 DESD-PSKTYLP*KMSSYETLRVVYSLAPLFITTLNFR*LHYWED------IKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK 662
            DESD P  TYL  K   YETLR+  + APL +        H   D       KVPRDTIVLINAWAIHRDPKSWSEAT+FKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALL+QCFDWKRTSDD IDMAERDGFVLTKLIPLKAMCKTRPVVN++ K
Sbjct:  343 DESDLPKLTYL--KNVIYETLRLC-TPAPLLLP-------HSTSDDCIIGGYKVPRDTIVLINAWAIHRDPKSWSEATSFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLVQCFDWKRTSDDKIDMAERDGFVLTKLIPLKAMCKTRPVVNKVFK 498
[Close]
BLAST of HO064581 vs. TrEMBL
Match: Q6WNR0_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 218.394 bits (555), Expect = 4.459e-55
Identity = 118/177 (66.67%), Postives = 134/177 (75.71%), Query Frame = -2 
Query:  186 DTHVRTRSFG*DESD-PSKTYLP*KMSSYETLRVVYSLAPLFITTLNFR*LHYWED------IKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK 695
            DTHV       DESD P  TYL  +   YETLR+ Y+ APL +        H   D       KVPRDTIVLINAWAIHRDP++WSEATTFKPERFDKKGE+EK+IAFGMGRR CPGE LALR ISMTLALL+QCFDWKR +D+ IDM+ERDGF +TKL+PLKAMCKTRPVVN++ K
Sbjct:  333 DTHVGQDRLV-DESDLPKLTYL--RNVIYETLRL-YTPAPLLLP-------HSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDKKGELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGFTMTKLLPLKAMCKTRPVVNKVFK 498
[Close]
BLAST of HO064581 vs. TrEMBL
Match: Q9ZWF2_GLYEC (Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-31 PE=2 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 6.026e-52
Identity = 114/177 (64.41%), Postives = 131/177 (74.01%), Query Frame = -2 
Query:  186 DTHVRTRSFG*DESD-PSKTYLP*KMSSYETLRVVYSLAPLFITTLNFR*LHYWED------IKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK 695
            DTHV       DESD P  +YL  K    ETLR+ Y+ APL +        H   D       KVP+DTIVLINAWAIHRDP+ W+EATTFKPERF+KKGE+EK+IAFGMGRR CPGE LA+R ISMTLALLIQCFDWK T+ D IDMAERDGF LTKL+PLKAMCK+RPV+N++ K
Sbjct:  333 DTHVGQDRLV-DESDLPKLSYL--KNVINETLRL-YTPAPLLLP-------HSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLTNGDKIDMAERDGFTLTKLVPLKAMCKSRPVINKVFK 498
[Close]
BLAST of HO064581 vs. TrEMBL
Match: Q9MBE4_LOTJA (Cytochrome P450 OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 6.663e-51
Identity = 113/177 (63.84%), Postives = 128/177 (72.32%), Query Frame = -2 
Query:  186 DTHVRTRSFG*DESD-PSKTYLP*KMSSYETLRVVYSLAPLFITTLNFR*LHYWED------IKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK 695
            DTHV       DESD P  TYL  K    ETLR+ Y+ APL +        H   D       KVPRDTIVLINAWA+HRDP+ W+EATTFKPERFDKKGE+EK+I FG+GRR CPGE LA+R ISMTLALLIQCFDWKR SD+ IDM ERDGFVL K IP+KAMCK+RPV+N + K
Sbjct:  334 DTHVGQDRLV-DESDIPKLTYL--KNVINETLRL-YTPAPLLLP-------HSASDDCTIGGYKVPRDTIVLINAWALHRDPQLWTEATTFKPERFDKKGELEKLIPFGLGRRACPGELLAIRAISMTLALLIQCFDWKRVSDEEIDMGERDGFVLMKSIPVKAMCKSRPVINNVFK 499
[Close]
BLAST of HO064581 vs. TrEMBL
Match: Q2LAL0_SOYBN (Cytochrome P450 monooxygenase CYP81E11 OS=Glycine max GN=CYP81E11 PE=2 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 5.850e-39
Identity = 82/158 (51.90%), Postives = 114/158 (72.15%), Query Frame = -2 
Query:  192 DESD-PSKTYLP*KMSSYETLRVVYSLAPLFITTLNFR*LHYWEDIKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRI 662
            +ESD P   YL  +    ETLR+ Y  AP+ I  +    ++  E   VPRDTIV+IN WA+ RDPK W +AT+FKPERFD++GE +K++AFGMGRR CPGE +A++++S TL L+IQCFDWKR S+  +DM E +   L++LIPL+AMCK RP+ +++
Sbjct:  347 NESDLPKLPYL--RKIVLETLRL-YPPAPILIPHVASEDINI-EGFNVPRDTIVIINGWAMQRDPKIWKDATSFKPERFDEEGEEKKLVAFGMGRRACPGEPMAMQSVSYTLGLMIQCFDWKRVSEKKLDMTENNWITLSRLIPLEAMCKARPLASKV 500
[Close]
BLAST of HO064581 vs. TrEMBL
Match: Q2LAK9_SOYBN (Cytochrome P450 monooxygenase CYP81E10 OS=Glycine max GN=CYP82E13 PE=2 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 3.792e-38
Identity = 81/158 (51.27%), Postives = 112/158 (70.89%), Query Frame = -2 
Query:  192 DESD-PSKTYLP*KMSSYETLRVVYSLAPLFITTLNFR*LHYWEDIKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRI 662
            +ESD P   YL  +    ETLR+ Y  AP+ I  ++   +   E   +PRDTIV+IN W + RDP+ W++AT FKPERFD +GE +K++AFGMGRR CPGE +A++++S TL LLIQCFDWKR S++ +DM E +   L++LIPL+AMCK RP+  +I
Sbjct:  347 NESDLPKLPYL--RKIILETLRL-YPPAPILIPHVSSEDITI-EGFNIPRDTIVIINGWGMQRDPQLWNDATCFKPERFDVEGEEKKLVAFGMGRRACPGEPMAMQSVSFTLGLLIQCFDWKRVSEEKLDMTENNWITLSRLIPLEAMCKARPLATKI 500
[Close]
BLAST of HO064581 vs. TrEMBL
Match: B7FM85_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 5.063e-36
Identity = 71/112 (63.39%), Postives = 89/112 (79.46%), Query Frame = -2 
Query:  192 VPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRP-VVNRI 524
            VP++TI+L+NAWAIHRD + WS+ T FKPERFDK+GE +K+I FG+GRR CPG  L  RT+S+TLALLIQCFDWKR S++ IDM E  G    KLIPL+AMCK R  V+N++
Sbjct:  390 VPKNTILLVNAWAIHRDSQLWSDPTQFKPERFDKEGEADKLIPFGLGRRACPGANLGQRTVSLTLALLIQCFDWKRISEEEIDMTEGKGATTPKLIPLEAMCKARSNVINKV 501

HSP 2 Score: 22.3274 bits (46), Expect = 5.063e-36
Identity = 9/18 (50.00%), Postives = 10/18 (55.56%), Query Frame = -3 
Query:  536 PWLHCLLPHSTSDDCIIG 589
            P    L PH +S DC IG
Sbjct:  369 PAFALLAPHFSSQDCTIG 386
[Close]
BLAST of HO064581 vs. TrEMBL
Match: Q9SML1_CICAR (Cytochrome P450 monooxygenase (Fragment) OS=Cicer arietinum GN=cyp81E4 PE=2 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 1.033e-35
Identity = 67/112 (59.82%), Postives = 88/112 (78.57%), Query Frame = -2 
Query:  189 VPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRII 524
            VP+DTI+L N WAIHRDPK WS+A++FKPERF+  GE+ K+IAFG+GRR CPG++LA RT+ +TL LLIQCF+WKR +++ ID+ E  G  +   +PLKAMCK  P+ N II
Sbjct:   95 VPKDTIILTNVWAIHRDPKYWSDASSFKPERFEIDGELNKLIAFGLGRRACPGQSLAHRTVGLTLGLLIQCFEWKRETEEKIDLREGKGLTMPMGVPLKAMCKPLPIANYII 206
[Close]
BLAST of HO064581 vs. TrEMBL
Match: Q9SML2_CICAR (Cytochrome P450 monooxygenase (Fragment) OS=Cicer arietinum GN=cyp81E5 PE=2 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 8.742e-35
Identity = 74/148 (50.00%), Postives = 100/148 (67.57%), Query Frame = -2 
Query:  186 ETLRVVYSLAPLFITTLNFR*LHYWED------IKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRIIK 611
            ETLR+ +  APL +        HY  +        VP+DTI+L N WAIHRDP  WS+  +FKPERF+ +GE+ K+IAFG+GRR CPG  LA R++ +T+ +LIQCF+WKR SD+ +DM E  G  + K +PL+AMCK  P+VN I+K
Sbjct:  101 ETLRL-HPPAPLLLP-------HYSSEDFTLGGFNVPKDTIILSNVWAIHRDPNLWSDPLSFKPERFEIEGELNKLIAFGLGRRACPGLNLAQRSVGLTVGMLIQCFEWKRESDEKLDMMEDKGITMPKKVPLEAMCKPLPIVNDIMK 240
[Close]
BLAST of HO064581 vs. TAIR peptide
Match: AT4G37330.1 (| Symbols: CYP81D4 | cytochrome P450, family 81, subfamily D, polypeptide 4 | chr4:17562547-17564569 REVERSE LENGTH=492)

HSP 1 Score: 147.132 bits (370), Expect = 8.604e-36
Identity = 74/146 (50.68%), Postives = 96/146 (65.75%), Query Frame = -2 
Query:  189 ETLRVVYSLAPLFITTLNFR*LHYWEDIKV-----PRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRII 611
            ETLR+ Y   PL +  L+       ED KV     P  T+VL NAWA+HRDP+ W +   FKPERF+K+GE EK+I+FGMGRR CPG  LA R I+  L  L+QCF+W+R  +D +DM E  G  L K IPL+AMCK R +V+++I
Sbjct:  354 ETLRM-YPAVPLLLPHLSS------EDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEKEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPKAIPLRAMCKARSIVDKLI 492
[Close]
BLAST of HO064581 vs. TAIR peptide
Match: AT4G37340.1 (| Symbols: CYP81D3 | cytochrome P450, family 81, subfamily D, polypeptide 3 | chr4:17564953-17566706 REVERSE LENGTH=500)

HSP 1 Score: 144.821 bits (364), Expect = 4.270e-35
Identity = 73/147 (49.66%), Postives = 99/147 (67.35%), Query Frame = -2 
Query:  189 ETLRVVYSLAPLFITTLNFR*LHYWEDIKV-----PRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAE-RDGFVLTKLIPLKAMCKTRPVVNRII 611
            E+LR+ Y  +PL +  +        ED KV     PR T++L NAWAIHRDPK W + T+FKPERF+K+GE +K++ FG+GRR CPG  LA R  S+T+  LIQCF+W+R  ++ +DM E   G ++ K IPL AMCK RPVV +I+
Sbjct:  357 ESLRL-YPASPLLVPHVAS------EDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFEKEGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPKAIPLVAMCKARPVVGKIL 496
[Close]
BLAST of HO064581 vs. TAIR peptide
Match: AT3G28740.1 (| Symbols: CYP81D1 | Cytochrome P450 superfamily protein | chr3:10788764-10790552 REVERSE LENGTH=509)

HSP 1 Score: 140.198 bits (352), Expect = 1.052e-33
Identity = 68/142 (47.89%), Postives = 96/142 (67.61%), Query Frame = -2 
Query:  189 ETLRVVYSLAPLFITTLNFR*LHYWEDIKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERD-GFVLTKLIPLKAMCKTRPVVNRII 611
            ETLR+ Y +AP+ +  L        +   VPR TI+L+NAWAIHRDPK W E   FKPERF+KKGE +K++ FG+GRR CPG  LA R +++ L  L+QCF+W+R  +  +DM E + G  + K   L+AMCK RP+V++++
Sbjct:  365 ETLRL-YPVAPMLLPHLASEDCIV-DGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEKKGEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSLQAMCKARPIVHKVL 504
[Close]
BLAST of HO064581 vs. TAIR peptide
Match: AT5G36220.1 (| Symbols: CYP81D1, CYP91A1 | cytochrome p450 81d1 | chr5:14253827-14256015 REVERSE LENGTH=502)

HSP 1 Score: 137.502 bits (345), Expect = 2.272e-33
Identity = 62/121 (51.24%), Postives = 85/121 (70.25%), Query Frame = -2 
Query:  189 EDIKV-----PRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRII 536
            ED K+     PR T +L+NAWAIHRDP +W +  +FKPERF+K+ E +K++AFG+GRR CPG  LA R + + L  LIQCF+W+R  +  +DM E  G  + K IPLKA+CK RP +++II
Sbjct:  381 EDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLHKII 501

HSP 2 Score: 22.7126 bits (47), Expect = 2.272e-33
Identity = 8/13 (61.54%), Postives = 10/13 (76.92%), Query Frame = -3 
Query:  536 LLPHSTSDDCIIG 574
            L+PH  S+DC IG
Sbjct:  374 LVPHMASEDCKIG 386
[Close]
BLAST of HO064581 vs. TAIR peptide
Match: AT4G37410.1 (| Symbols: CYP81F4 | cytochrome P450, family 81, subfamily F, polypeptide 4 | chr4:17590848-17592780 FORWARD LENGTH=501)

HSP 1 Score: 139.043 bits (349), Expect = 2.343e-33
Identity = 62/124 (50.00%), Postives = 89/124 (71.77%), Query Frame = -2 
Query:  192 EDIK-----VPRDTIVLINAWAIHRDPKSWSEATTFKPERFD----KKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRI 536
            EDIK     VPRDT+V++NAWAIHRDP  W+E   F PERF+    +K ++  +IAFG GRR+CPG  LA + +++ L  LIQCFDWK+ ++  IDM+E  G  +  ++PL+A+CKTRP++N++
Sbjct:  372 EDIKIGGYDVPRDTMVMVNAWAIHRDPDLWTEPERFNPERFNGGEGEKDDVRMLIAFGSGRRICPGVGLAHKIVTLALGSLIQCFDWKKVNEKEIDMSEGPGMAMRMMVPLRALCKTRPIMNKL 495
[Close]
BLAST of HO064581 vs. TAIR peptide
Match: AT4G37360.1 (| Symbols: CYP81D2 | cytochrome P450, family 81, subfamily D, polypeptide 2 | chr4:17567124-17568858 REVERSE LENGTH=499)

HSP 1 Score: 139.043 bits (349), Expect = 2.343e-33
Identity = 74/164 (45.12%), Postives = 106/164 (64.63%), Query Frame = -2 
Query:  189 DESD-PSKTYLP*KMSSYETLRVVYSLAPLFITTLNFR*LHYWEDIKV-----PRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRII 662
            +ESD P+  YL   +S  ETLR+ Y  AP+ +  +  +      D KV     PR T++L NAWAIHRDP  W + T+FKPERF+K+GE +K++ FG+GRR CPG  LA R ++++L  LIQCF+W+R  ++ +DM E  G  + K  PL+AMC+ R  V +I+
Sbjct:  341 EESDIPNLPYLQNIVS--ETLRL-YPAAPMLLPHVASK------DCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEAMCRARDFVGKIL 495
[Close]
BLAST of HO064581 vs. TAIR peptide
Match: AT1G66540.2 (| Symbols: | Cytochrome P450 superfamily protein | chr1:24825446-24826502 FORWARD LENGTH=327)

HSP 1 Score: 139.043 bits (349), Expect = 2.343e-33
Identity = 73/163 (44.79%), Postives = 103/163 (63.19%), Query Frame = -2 
Query:  192 DESD-PSKTYLP*KMSSYETLRVVYSLAPLFITTLNFR*LHYWEDIKV-----PRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRI 662
            +ESD P+  YL   +S  ETLR+ Y   PL +  ++       ED KV     P  T++L+N WAIHRDP+ W +  +FKPERF+K+GE  K++ FG+GRR CPG  LA R +S++L  LIQCF+W+R  ++ +DM E  G  + + IPL AMC+ R  V +I
Sbjct:  169 EESDIPNLPYLQNIVS--ETLRL-YPAGPLLVPHISS------EDCKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPERFEKEGETHKLLTFGLGRRACPGSGLARRLVSLSLGSLIQCFEWERIGEEEVDMTEGGGLTMPRAIPLVAMCRARAFVGKI 322
[Close]
BLAST of HO064581 vs. TAIR peptide
Match: AT1G66540.1 (| Symbols: | Cytochrome P450 superfamily protein | chr1:24824837-24826502 FORWARD LENGTH=386)

HSP 1 Score: 139.043 bits (349), Expect = 2.343e-33
Identity = 73/163 (44.79%), Postives = 103/163 (63.19%), Query Frame = -2 
Query:  192 DESD-PSKTYLP*KMSSYETLRVVYSLAPLFITTLNFR*LHYWEDIKV-----PRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRI 662
            +ESD P+  YL   +S  ETLR+ Y   PL +  ++       ED KV     P  T++L+N WAIHRDP+ W +  +FKPERF+K+GE  K++ FG+GRR CPG  LA R +S++L  LIQCF+W+R  ++ +DM E  G  + + IPL AMC+ R  V +I
Sbjct:  228 EESDIPNLPYLQNIVS--ETLRL-YPAGPLLVPHISS------EDCKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPERFEKEGETHKLLTFGLGRRACPGSGLARRLVSLSLGSLIQCFEWERIGEEEVDMTEGGGLTMPRAIPLVAMCRARAFVGKI 381
[Close]
BLAST of HO064581 vs. TAIR peptide
Match: AT4G37370.1 (| Symbols: CYP81D8 | cytochrome P450, family 81, subfamily D, polypeptide 8 | chr4:17569971-17571678 REVERSE LENGTH=497)

HSP 1 Score: 133.65 bits (335), Expect = 9.844e-32
Identity = 76/163 (46.63%), Postives = 104/163 (63.80%), Query Frame = -2 
Query:  192 DESDPSKT-YLP*KMSSYETLRVVYSLAPLFITTLNFR*LHYWEDIKV-----PRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRI 662
            DESD S   YL   +S  ETLR+ Y  AP+ +  +        ED KV     PR TI+L N WAIHRDP+ W +  +FKPERF+K+GE +K++ FG+GRR CPG  LA R I++TL  LIQC +W++  ++ +DM+E  G  + K  PL+AMC+ RP V +I
Sbjct:  340 DESDISNLPYLQNIVS--ETLRL-YPAAPMLLPHVAS------EDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAKPLEAMCRARPSVVKI 492
[Close]
BLAST of HO064581 vs. TAIR peptide
Match: AT4G37320.1 (| Symbols: CYP81D5 | cytochrome P450, family 81, subfamily D, polypeptide 5 | chr4:17559742-17561690 REVERSE LENGTH=495)

HSP 1 Score: 131.724 bits (330), Expect = 3.741e-31
Identity = 65/147 (44.22%), Postives = 88/147 (59.86%), Query Frame = -2 
Query:  189 ETLRVVYSLAPLFITTLNFR*LHYWED------IKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLIQCFDWKRTSDDMIDMAERDGFVLTKLIPLKAMCKTRPVVNRII 611
            ETLR+ Y   PL +        H+  D        +PR T++L N WA+HRDP  W E   FKPERF+K+GE  K++ FGMGRR CPG  L  R +S+ L  LIQ F+W+R   +++DM E +G  + K  PL+AMCK R +V + I
Sbjct:  357 ETLRL-YPAVPLLLP-------HFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERFEKEGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITMPKATPLRAMCKARAIVGKTI 495
The following BLAST results are available for this feature:
BLAST of HO064581 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10
Match NameE-valueIdentityDescription
C81E1_GLYEC1.554e-5263.84Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata ... [more]
C81D1_ARATH2.266e-3251.24Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CY... [more]
C81F1_ARATH1.465e-2645.69Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CY... [more]
C71A8_MENPI5.046e-1947.66Cytochrome P450 71A8 OS=Mentha piperita GN=CYP71A8... [more]
C76C2_ARATH2.504e-1844.04Cytochrome P450 76C2 OS=Arabidopsis thaliana GN=CY... [more]
C71BO_ARATH3.271e-1837.50Cytochrome P450 71B24 OS=Arabidopsis thaliana GN=C... [more]
C76B1_HELTU5.579e-1842.457-ethoxycoumarin O-deethylase OS=Helianthus tubero... [more]
C76C4_ARATH7.286e-1841.88Cytochrome P450 76C4 OS=Arabidopsis thaliana GN=CY... [more]
C93A2_SOYBN9.516e-1838.66Cytochrome P450 93A2 OS=Glycine max GN=CYP93A2 PE=... [more]
C76C1_ARATH9.516e-1843.22Cytochrome P450 76C1 OS=Arabidopsis thaliana GN=CY... [more]
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BLAST of HO064581 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q9XFX0_CICAR4.163e-6175.71Cytochrome P450 monooxygenase OS=Cicer arietinum G... [more]
Q9ZRW6_CICAR1.339e-5976.51Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2... [more]
Q6WNR0_MEDTR4.459e-5566.67Isoflavone 2'-hydroxylase OS=Medicago truncatula P... [more]
Q9ZWF2_GLYEC6.026e-5264.41Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-... [more]
Q9MBE4_LOTJA6.663e-5163.84Cytochrome P450 OS=Lotus japonicus PE=2 SV=1[more]
Q2LAL0_SOYBN5.850e-3951.90Cytochrome P450 monooxygenase CYP81E11 OS=Glycine ... [more]
Q2LAK9_SOYBN3.792e-3851.27Cytochrome P450 monooxygenase CYP81E10 OS=Glycine ... [more]
B7FM85_MEDTR5.063e-3663.39Putative uncharacterized protein OS=Medicago trunc... [more]
Q9SML1_CICAR1.033e-3559.82Cytochrome P450 monooxygenase (Fragment) OS=Cicer ... [more]
Q9SML2_CICAR8.742e-3550.00Cytochrome P450 monooxygenase (Fragment) OS=Cicer ... [more]
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BLAST of HO064581 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10
Match NameE-valueIdentityDescription
AT4G37330.18.604e-3650.68| Symbols: CYP81D4 | cytochrome P450, family 81, s... [more]
AT4G37340.14.270e-3549.66| Symbols: CYP81D3 | cytochrome P450, family 81, s... [more]
AT3G28740.11.052e-3347.89| Symbols: CYP81D1 | Cytochrome P450 superfamily p... [more]
AT5G36220.12.272e-3351.24| Symbols: CYP81D1, CYP91A1 | cytochrome p450 81d1... [more]
AT4G37410.12.343e-3350.00| Symbols: CYP81F4 | cytochrome P450, family 81, s... [more]
AT4G37360.12.343e-3345.12| Symbols: CYP81D2 | cytochrome P450, family 81, s... [more]
AT1G66540.22.343e-3344.79| Symbols: | Cytochrome P450 superfamily protein ... [more]
AT1G66540.12.343e-3344.79| Symbols: | Cytochrome P450 superfamily protein ... [more]
AT4G37370.19.844e-3246.63| Symbols: CYP81D8 | cytochrome P450, family 81, s... [more]
AT4G37320.13.741e-3144.22| Symbols: CYP81D5 | cytochrome P450, family 81, s... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450GENE3D1.10.630.10no descriptioncoord: 5..113
score: 4.2
IPR001128Cytochrome P450PANTHERPTHR19383CYTOCHROME P450coord: 9..80
score: 9.6
IPR001128Cytochrome P450PFAMPF00067p450coord: 6..102
score: 1.7
IPR001128Cytochrome P450SUPERFAMILY48264Cytochrome P450coord: 4..113
score: 5.4
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 18..42
score: 2.8e-14coord: 49..59
score: 2.8e-14coord: 59..82
score: 2.8
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 52..61
score
NoneNo IPR availablePANTHERPTHR19383:SF42CYTOCHROME P450 SUBFAMILY 81Dcoord: 9..80
score: 9.6

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>HO064581 ID=HO064581; Name=HO064581; organism=Cicer arietinum; type=EST; length=705bp
GTAAAATAAAATAACTTTTTATTTAAGAAAACGGAGTTAAAAATGTATAC
AATGATGGCTCATAGCTTTTTCATACTAAGGATACAATATAACACTTATT
TGAACATGCATATATAGTACTTGCGTTGTATTGAATACACATTTCGAAGA
ACTCTAGAACCGGAAAGTACCGCGAGAATTAATTACTTGATAATTCTGTT
TACGACCGGACGAGTTTTACACATGGCCTTTAATGGAATTAATTTTGTCA
AAACAAATCCATCTCGTTCTGCCATATCAATCATGTCATCACTTGTACGT
TTCCAATCAAAGCATTGAATCAATAATGCCAAAGTCATGCTAATTGTACG
AAGAGCCAAAGCTTCTCCAGGACACACTCTTCTTCCCATCCCAAATGCAA
TCACCTTTTCAATCTCCCCTTTTTTGTCAAACCTCTCCGGCTTGAAGGTT
GTGGCCTCACTCCATGACTTAGGATCCCTGTGAATGGCCCATGCATTGAT
CAATACTATGGTGTCTCTTGGTACTTTTATATCCTCCCAATAATGCAATC
ATCTGAAGTTGAGTGTGGTAATAAACAATGGAGCCAGGGAGTACACAACC
CGAAGCGTTTCATAAGATGACATTTTTTAGGGTAAGTAAGTTTTGGAGGG
GTCTGATTCATCCTACCCAAACGATCTTGTCCTAACATGAGTATCCCAAT
TTCAT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004497monooxygenase activity
GO:0005506iron ion binding
GO:0009055electron transfer activity
GO:0020037heme binding
Vocabulary: Biological Process
TermDefinition
GO:0055114obsolete oxidation-reduction process
Vocabulary: INTERPRO
TermDefinition
IPR001128Cyt_P450
IPR002401Cyt_P450_E_grp-I
IPR017972Cyt_P450_CS