FL512332

EST Overview


Name	FL512332
Unique Name	FL512332
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	502

Libraries


Library Name	Type
LIBEST_023567 Subtracted cDNA library of drought-stressed chickpea cultivar	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FL512332 vs. SwissProt
Match: NOG1_ARATH (Probable nucleolar GTP-binding protein 1 OS=Arabidopsis thaliana GN=At1g50920 PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 5.462e-16
Identity = 50/126 (39.68%), Postives = 67/126 (53.17%), Query Frame = 3

Query:    3 TAERMGRQLSSLGLDPSLAIKECAADLYLGKAGKGRDH-QVLMVSGVDIDGDTPSKKQXXXXXXXXXXXX-------PPHEVVPGEGLRDSAQN-QGIKLAKKSVKKRNKDARRGEADRSFLMLNP 353
            T +RMGR+LS++GLDPS A+    +       G+ RD  +      +D+D +  S K+                   P HEVVPGEG +DS Q    IK++ KS KKR+K+ARRGEADR    L P
Sbjct:  534 TTKRMGRELSAMGLDPSSAMDRARSK----SRGRKRDRSEDAGNDAMDVDDEQQSNKKQRVRSKSRAMSISRSQSRPPAHEVVPGEGFKDSTQKLSAIKISNKSHKKRDKNARRGEADRVIPTLRP 655

HSP 2 Score: 28.1054 bits (61), Expect = 5.462e-16
Identity = 9/15 (60.00%), Postives = 14/15 (93.33%), Query Frame = 2

Query:  332 IIPNVKPKHLYTGKR 376
            +IP ++PKHL++GKR
Sbjct:  649 VIPTLRPKHLFSGKR 663

[Close]

BLAST of FL512332 vs. TrEMBL
Match: B9RIE0_RICCO (Nucleolar GTP-binding protein, putative OS=Ricinus communis GN=RCOM_1578360 PE=4 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 6.335e-21
Identity = 57/118 (48.31%), Postives = 68/118 (57.63%), Query Frame = 3

Query:    3 TAERMGRQLSSLGLDPSLAIKECAADLYLGKAGKGRDHQVLMV-------SGVDIDGDTPSKKQXXXXXXXXXXXXPPHEVVPGEGLRDSAQN-QGIKLAKKSVKKRNKDARRGEADR 332
            T +RMGR L S+GLDPSLAI    +        +GR  +  +          +D+D D P+KK             PP EVVPGEG +DSAQ  +GIKL KKS KKRNKDARRGEADR
Sbjct:  542 TTDRMGRDLKSVGLDPSLAINRVRSR----SVSRGRKRERSLAREDKDGGDAMDMDVDQPNKKLRLAISRSRSKSRPPTEVVPGEGFKDSAQKIKGIKLHKKSAKKRNKDARRGEADR 655

HSP 2 Score: 30.0314 bits (66), Expect = 6.335e-21
Identity = 11/15 (73.33%), Postives = 13/15 (86.67%), Query Frame = 2

Query:  332 IIPNVKPKHLYTGKR 376
            +IP  +PKHLYTGKR
Sbjct:  656 VIPTERPKHLYTGKR 670

[Close]

BLAST of FL512332 vs. TrEMBL
Match: B9GF11_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_549253 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.936e-19
Identity = 56/123 (45.53%), Postives = 72/123 (58.54%), Query Frame = 3

Query:    3 TAERMGRQLSSLGLDPSLAIKECAADLYLGKAGKGRDHQVLMV----SGVDIDGDTPSKK-QXXXXXXXXXXXXPPHEVVPGEGLRDSAQ-NQGIKLAKKSVKKRNKDARRGEADRSFLMLNP 353
            T +RMGRQLS++G+DPS AI     +  L   G+ R+            +D+D D P+KK +            PP+EVVPGEG +DSAQ ++ +KL K S KKRNKDARRGEADR    L P
Sbjct:  492 TTKRMGRQLSAMGVDPSKAINRLR-ERSLSTRGRKRERSTDRGHDDGDAMDMDVDQPNKKLRLRSTSRSRSRSRPPNEVVPGEGFKDSAQKSKALKLFKNSAKKRNKDARRGEADRVIPTLKP 613

HSP 2 Score: 30.8018 bits (68), Expect = 1.936e-19
Identity = 11/16 (68.75%), Postives = 15/16 (93.75%), Query Frame = 2

Query:  332 IIPNVKPKHLYTGKRS 379
            +IP +KPKHL++GKRS
Sbjct:  607 VIPTLKPKHLFSGKRS 622

[Close]

BLAST of FL512332 vs. TrEMBL
Match: A5BG44_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_024726 PE=4 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 9.356e-19
Identity = 62/126 (49.21%), Postives = 74/126 (58.73%), Query Frame = 3

Query:    3 TAERMGRQLSSLGLDPSLAIKECAADLYLGKAGK-----GRDHQVLMVSGVDIDGDTPSKK---QXXXXXXXXXXXXPPHEVVPGEGLRDSAQN-QGIKLAKKSVKKRNKDARRGEADRSFLMLNP 353
            T+ERMGRQLS+LGLDP+LAI    +     K  +     G D  V+M    D+D D P+KK   +            PP EVV GEG +DSAQ  + +KL KKSVKKRNKDARRGEADR    L P
Sbjct:  539 TSERMGRQLSALGLDPTLAINRARSKSRGRKRERSLDRGGNDGDVVM----DMDVDXPNKKLRARSRSLSRLRSMSRPPGEVVAGEGFKDSAQKVKALKLGKKSVKKRNKDARRGEADRVIPTLKP 660

HSP 2 Score: 22.7126 bits (47), Expect = 9.356e-19
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 2

Query:  332 IIPNVKPKHLY 364
            +IP +KPKHL+
Sbjct:  654 VIPTLKPKHLF 664

[Close]

BLAST of FL512332 vs. TrEMBL
Match: Q8LIM1_ORYSJ (Putative GTP binding protein OS=Oryza sativa subsp. japonica GN=OJ1567_G09.130 PE=4 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 2.676e-18
Identity = 54/119 (45.38%), Postives = 65/119 (54.62%), Query Frame = 3

Query:    3 TAERMGRQLSSLGLDPSLAIKECAADLYLGKAGKGRDHQVLMVSGVDIDGDTPSKK-QXXXXXXXXXXXXPPHEVVPGEGLRDSAQNQ-GIKLAKKSVKKRNKDARRGEADRSFLMLNP 353
            T +RMGRQLSS+G DPS A+    +     K  +           +DIDG   SKK +            PP EVVPGEG +DSAQ +  IK AK SV+ RNK+ARRGEADR    L P
Sbjct:  542 TTDRMGRQLSSMGFDPSAAMDRARSRSRGRKRERSLSRAASDGDDMDIDGQQSSKKLRALSRSRSRSKSRPPEEVVPGEGFKDSAQKKKAIKKAKDSVRNRNKEARRGEADRVIPTLKP 660

HSP 2 Score: 28.4906 bits (62), Expect = 2.676e-18
Identity = 10/16 (62.50%), Postives = 14/16 (87.50%), Query Frame = 2

Query:  332 IIPNVKPKHLYTGKRS 379
            +IP +KPKHL++GK S
Sbjct:  654 VIPTLKPKHLFSGKSS 669

[Close]

BLAST of FL512332 vs. TrEMBL
Match: Q69YA7_ORYSJ (Putative nucleolar GTP-binding protein OS=Oryza sativa subsp. japonica GN=P0528E04.18 PE=4 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 2.676e-18
Identity = 53/119 (44.54%), Postives = 63/119 (52.94%), Query Frame = 3

Query:    3 TAERMGRQLSSLGLDPSLAIKECAADLYLGKAGKGRDHQVLMVSGVDIDGDTPSKK-QXXXXXXXXXXXXPPHEVVPGEGLRDSAQNQ-GIKLAKKSVKKRNKDARRGEADRSFLMLNP 353
            T  RMGRQLSS+G DP  A+    +     K  +           +DIDG   SKK +            PP EVVPGEG +DSAQ +  IK AK SV+ RNK+ARRGEADR    L P
Sbjct:  542 TTNRMGRQLSSMGFDPRAALDRARSRSRGRKRERSLSRAASDGDDMDIDGQQSSKKLRALSRSRSRSKSRPPEEVVPGEGFKDSAQKKKAIKKAKDSVRNRNKEARRGEADRVIPTLKP 660

HSP 2 Score: 30.8018 bits (68), Expect = 2.676e-18
Identity = 11/16 (68.75%), Postives = 15/16 (93.75%), Query Frame = 2

Query:  332 IIPNVKPKHLYTGKRS 379
            +IP +KPKHL++GKRS
Sbjct:  654 VIPTLKPKHLFSGKRS 669

[Close]

BLAST of FL512332 vs. TrEMBL
Match: Q0D929_ORYSJ (Os07g0110200 protein OS=Oryza sativa subsp. japonica GN=Os07g0110200 PE=4 SV=2)

HSP 1 Score: 87.0409 bits (214), Expect = 2.716e-18
Identity = 54/119 (45.38%), Postives = 65/119 (54.62%), Query Frame = 3

Query:    3 TAERMGRQLSSLGLDPSLAIKECAADLYLGKAGKGRDHQVLMVSGVDIDGDTPSKK-QXXXXXXXXXXXXPPHEVVPGEGLRDSAQNQ-GIKLAKKSVKKRNKDARRGEADRSFLMLNP 353
            T +RMGRQLSS+G DPS A+    +     K  +           +DIDG   SKK +            PP EVVPGEG +DSAQ +  IK AK SV+ RNK+ARRGEADR    L P
Sbjct:  210 TTDRMGRQLSSMGFDPSAAMDRARSRSRGRKRERSLSRAASDGDDMDIDGQQSSKKLRALSRSRSRSKSRPPEEVVPGEGFKDSAQKKKAIKKAKDSVRNRNKEARRGEADRVIPTLKP 328

HSP 2 Score: 28.4906 bits (62), Expect = 2.716e-18
Identity = 10/16 (62.50%), Postives = 14/16 (87.50%), Query Frame = 2

Query:  332 IIPNVKPKHLYTGKRS 379
            +IP +KPKHL++GK S
Sbjct:  322 VIPTLKPKHLFSGKSS 337

[Close]

BLAST of FL512332 vs. TrEMBL
Match: A2YHG3_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_24631 PE=4 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 2.716e-18
Identity = 54/119 (45.38%), Postives = 65/119 (54.62%), Query Frame = 3

Query:    3 TAERMGRQLSSLGLDPSLAIKECAADLYLGKAGKGRDHQVLMVSGVDIDGDTPSKK-QXXXXXXXXXXXXPPHEVVPGEGLRDSAQNQ-GIKLAKKSVKKRNKDARRGEADRSFLMLNP 353
            T +RMGRQLSS+G DPS A+    +     K  +           +DIDG   SKK +            PP EVVPGEG +DSAQ +  IK AK SV+ RNK+ARRGEADR    L P
Sbjct:  208 TTDRMGRQLSSMGFDPSAAMDRARSRSRGRKRERSLSRAASDGDDMDIDGQQSSKKLRALSRSRSRSKSRPPEEVVPGEGFKDSAQKKKAIKKAKDSVRNRNKEARRGEADRVIPTLKP 326

HSP 2 Score: 28.4906 bits (62), Expect = 2.716e-18
Identity = 10/16 (62.50%), Postives = 14/16 (87.50%), Query Frame = 2

Query:  332 IIPNVKPKHLYTGKRS 379
            +IP +KPKHL++GK S
Sbjct:  320 VIPTLKPKHLFSGKSS 335

[Close]

BLAST of FL512332 vs. TrEMBL
Match: Q0DDV9_ORYSJ (Os06g0195900 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os06g0195900 PE=4 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 2.732e-18
Identity = 53/119 (44.54%), Postives = 63/119 (52.94%), Query Frame = 3

Query:    3 TAERMGRQLSSLGLDPSLAIKECAADLYLGKAGKGRDHQVLMVSGVDIDGDTPSKK-QXXXXXXXXXXXXPPHEVVPGEGLRDSAQNQ-GIKLAKKSVKKRNKDARRGEADRSFLMLNP 353
            T  RMGRQLSS+G DP  A+    +     K  +           +DIDG   SKK +            PP EVVPGEG +DSAQ +  IK AK SV+ RNK+ARRGEADR    L P
Sbjct:  143 TTNRMGRQLSSMGFDPRAALDRARSRSRGRKRERSLSRAASDGDDMDIDGQQSSKKLRALSRSRSRSKSRPPEEVVPGEGFKDSAQKKKAIKKAKDSVRNRNKEARRGEADRVIPTLKP 261

HSP 2 Score: 30.8018 bits (68), Expect = 2.732e-18
Identity = 11/16 (68.75%), Postives = 15/16 (93.75%), Query Frame = 2

Query:  332 IIPNVKPKHLYTGKRS 379
            +IP +KPKHL++GKRS
Sbjct:  255 VIPTLKPKHLFSGKRS 270

[Close]

BLAST of FL512332 vs. TrEMBL
Match: D7KKP1_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_888292 PE=4 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 1.682e-17
Identity = 52/124 (41.94%), Postives = 68/124 (54.84%), Query Frame = 3

Query:    3 TAERMGRQLSSLGLDPSLAIKECAADLYLGKAGKGRDHQVLMVSGVDIDGDTPSKKQXXXXXXXXXXXX------PPHEVVPGEGLRDSAQN-QGIKLAKKSVKKRNKDARRGEADRSFLMLNP 353
            T +RMGR+LSSLGLDPS A+   A     G+  +  D   +    VD++ D   KK+                  PPHEV+PGEG +DS+Q  + IK+  KS +KR+K ARRGEADR    L P
Sbjct:  530 TRKRMGRELSSLGLDPSSALNH-ARSKSRGRKRERYDGLSIDAMDVDVNDDEQQKKKMCLRSRSRSLSRSRSVSRPPHEVLPGEGFKDSSQKIKAIKIGHKSHRKRDKAARRGEADRVIPSLKP 652

HSP 2 Score: 31.9574 bits (71), Expect = 1.682e-17
Identity = 11/17 (64.71%), Postives = 16/17 (94.12%), Query Frame = 2

Query:  332 IIPNVKPKHLYTGKRSN 382
            +IP++KPKHL++GKR N
Sbjct:  646 VIPSLKPKHLFSGKRGN 662

[Close]

BLAST of FL512332 vs. TrEMBL
Match: Q9SY72_ARATH (F14N23.18 OS=Arabidopsis thaliana PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 3.702e-17
Identity = 53/125 (42.40%), Postives = 67/125 (53.60%), Query Frame = 3

Query:    3 TAERMGRQLSSLGLDPSLAIKECAADLYLGKAGKGRDHQVLMVSGVDIDGDTPSKKQXXXXXXXXXXXX-------PPHEVVPGEGLRDSAQN-QGIKLAKKSVKKRNKDARRGEADRSFLMLNP 353
            T +RMGR+LSSLGLDPS A+   A     G+  +  D        VD++ D   KK+                   PPHEVVPGEG +DS+Q  + IK+  KS +KR+K ARRGEADR    L P
Sbjct:  487 TRKRMGRELSSLGLDPSSALNR-ARSKSRGRKRERYDDLSNDAMDVDVNDDEQHKKKKMCLRSKSRSLSRSRSVSRPPHEVVPGEGFKDSSQKIKAIKIGHKSHRKRDKAARRGEADRVIPSLKP 610

HSP 2 Score: 31.9574 bits (71), Expect = 3.702e-17
Identity = 11/17 (64.71%), Postives = 16/17 (94.12%), Query Frame = 2

Query:  332 IIPNVKPKHLYTGKRSN 382
            +IP++KPKHL++GKR N
Sbjct:  604 VIPSLKPKHLFSGKRGN 620

[Close]

BLAST of FL512332 vs. TAIR peptide
Match: AT1G10300.1 (| Symbols: | Nucleolar GTP-binding protein | chr1:3378142-3380226 FORWARD LENGTH=687)

HSP 1 Score: 79.7221 bits (195), Expect = 3.177e-19
Identity = 53/125 (42.40%), Postives = 67/125 (53.60%), Query Frame = 3

Query:    3 TAERMGRQLSSLGLDPSLAIKECAADLYLGKAGKGRDHQVLMVSGVDIDGDTPSKKQXXXXXXXXXXXX-------PPHEVVPGEGLRDSAQN-QGIKLAKKSVKKRNKDARRGEADRSFLMLNP 353
            T +RMGR+LSSLGLDPS A+   A     G+  +  D        VD++ D   KK+                   PPHEVVPGEG +DS+Q  + IK+  KS +KR+K ARRGEADR    L P
Sbjct:  548 TRKRMGRELSSLGLDPSSALNR-ARSKSRGRKRERYDDLSNDAMDVDVNDDEQHKKKKMCLRSKSRSLSRSRSVSRPPHEVVPGEGFKDSSQKIKAIKIGHKSHRKRDKAARRGEADRVIPSLKP 671

HSP 2 Score: 31.9574 bits (71), Expect = 3.177e-19
Identity = 11/17 (64.71%), Postives = 16/17 (94.12%), Query Frame = 2

Query:  332 IIPNVKPKHLYTGKRSN 382
            +IP++KPKHL++GKR N
Sbjct:  665 VIPSLKPKHLFSGKRGN 681

[Close]

BLAST of FL512332 vs. TAIR peptide
Match: AT1G50920.1 (| Symbols: | Nucleolar GTP-binding protein | chr1:18870555-18872570 FORWARD LENGTH=671)

HSP 1 Score: 75.8702 bits (185), Expect = 6.021e-17
Identity = 50/126 (39.68%), Postives = 67/126 (53.17%), Query Frame = 3

Query:    3 TAERMGRQLSSLGLDPSLAIKECAADLYLGKAGKGRDH-QVLMVSGVDIDGDTPSKKQXXXXXXXXXXXX-------PPHEVVPGEGLRDSAQN-QGIKLAKKSVKKRNKDARRGEADRSFLMLNP 353
            T +RMGR+LS++GLDPS A+    +       G+ RD  +      +D+D +  S K+                   P HEVVPGEG +DS Q    IK++ KS KKR+K+ARRGEADR    L P
Sbjct:  534 TTKRMGRELSAMGLDPSSAMDRARSK----SRGRKRDRSEDAGNDAMDVDDEQQSNKKQRVRSKSRAMSISRSQSRPPAHEVVPGEGFKDSTQKLSAIKISNKSHKKRDKNARRGEADRVIPTLRP 655

HSP 2 Score: 28.1054 bits (61), Expect = 6.021e-17
Identity = 9/15 (60.00%), Postives = 14/15 (93.33%), Query Frame = 2

Query:  332 IIPNVKPKHLYTGKR 376
            +IP ++PKHL++GKR
Sbjct:  649 VIPTLRPKHLFSGKR 663

The following BLAST results are available for this feature:

BLAST of FL512332 vs. SwissProt
BLAST of FL512332 vs. TrEMBL
BLAST of FL512332 vs. TAIR peptide

BLAST of FL512332 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 1


Match Name	E-value	Identity	Description
NOG1_ARATH	5.462e-16	39.68	Probable nucleolar GTP-binding protein 1 OS=Arabid...	[more]

BLAST of FL512332 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9RIE0_RICCO	6.335e-21	48.31	Nucleolar GTP-binding protein, putative OS=Ricinus...	[more]
B9GF11_POPTR	1.936e-19	45.53	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A5BG44_VITVI	9.356e-19	49.21	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q8LIM1_ORYSJ	2.676e-18	45.38	Putative GTP binding protein OS=Oryza sativa subsp...	[more]
Q69YA7_ORYSJ	2.676e-18	44.54	Putative nucleolar GTP-binding protein OS=Oryza sa...	[more]
Q0D929_ORYSJ	2.716e-18	45.38	Os07g0110200 protein OS=Oryza sativa subsp. japoni...	[more]
A2YHG3_ORYSI	2.716e-18	45.38	Putative uncharacterized protein OS=Oryza sativa s...	[more]
Q0DDV9_ORYSJ	2.732e-18	44.54	Os06g0195900 protein (Fragment) OS=Oryza sativa su...	[more]
D7KKP1_ARALY	1.682e-17	41.94	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q9SY72_ARATH	3.702e-17	42.40	F14N23.18 OS=Arabidopsis thaliana PE=4 SV=1	[more]

BLAST of FL512332 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G10300.1	3.177e-19	42.40	\| Symbols: \| Nucleolar GTP-binding protein \| chr1...	[more]
AT1G50920.1	6.021e-17	39.68	\| Symbols: \| Nucleolar GTP-binding protein \| chr1...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 58..69 score
None	No IPR available	SEG	seg	seg	coord: 17..38 score
None	No IPR available	SEG	seg	seg	coord: 10..30 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FL512332 ID=FL512332; Name=FL512332; organism=Cicer arietinum; type=EST; length=502bp
ACACAGCAGAAAGAATGGGAAGGCAGCTGTCATCTCTGGGGCTTGATCCT
TCTCTAGCTATTAAAGAATGCGCAGCAGATCTGTATCTAGGCAAGGCCGG
AAAAGGGAGAGATCACCAAGTGTTGATGGTGAGTGGGGTGGATATTGATG
GTGACACACCTAGCAAGAAGCAGCGTCTGAGTAGATCACGTTCTAGGTCT
GTCTCCCGCCCACCACATGAAGTTGTGCCAGGAGAAGGTCTCAGGGATTC
TGCTCAGAATCAAGGAATAAAACTTGCAAAGAAATCTGTCAAGAAGAGAA
ACAAGGATGCCCGCCGTGGAGAAGCTGATAGATCATTCCTAATGTTAAAC
CCAAGCATTTGTATACTGGAAAACGCTCCAATGGAAAAACCGATAGACGA
AAAACAGGGGATGATGATGATGATGGCTTTGTGGTTGTCGAGAATTTTGT
TCTATTTATGTTTTCCTGAATGGCATGGTGATGGCTGCAAAAATTTTCCT
GT

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