GR916070

EST Overview


Name	GR916070
Unique Name	GR916070
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	465

Libraries


Library Name	Type
LIBEST_024969 Early immune responsive suppression subtracted cDNA libraries of Chickpea (Cicer arietinum cv. WR-315) wilt	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR916070 vs. SwissProt
Match: NIA_LOTJA (Nitrate reductase [NADH] OS=Lotus japonicus GN=NIA PE=3 SV=1)

HSP 1 Score: 237.654 bits (605), Expect = 1.960e-62
Identity = 110/140 (78.57%), Postives = 122/140 (87.14%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VR+FRFALP EDQQLGLP+GKHIFLC  +DGKLCMRAYTPTS VDE+GYF+LVVK+YFK VHPKFPNGG MSQ+LDSL IGS LDVKGP+GHIEY G GNF+V GK RFAKKL  +AGGTGITP+YQVAQAI  D +D T
Sbjct:  661 VRVFRFALPSEDQQLGLPVGKHIFLCATVDGKLCMRAYTPTSGVDEVGYFELVVKVYFKGVHPKFPNGGAMSQHLDSLPIGSDLDVKGPLGHIEYTGRGNFLVHGKHRFAKKLAMLAGGTGITPIYQVAQAILKDPEDHT 800

[Close]

BLAST of GR916070 vs. SwissProt
Match: NIA1_TOBAC (Nitrate reductase [NADH] 1 OS=Nicotiana tabacum GN=NIA1 PE=3 SV=1)

HSP 1 Score: 233.417 bits (594), Expect = 3.696e-61
Identity = 109/140 (77.86%), Postives = 120/140 (85.71%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VR FRFALP EDQ LGLP+GKHIFLC  ID KLCMRAYTPTS++DE+GYF+LVVKIYFK +HPKFPNGG MSQYLDSL +GS LDVKGP+GHIEY G GNF+V GKQ+FAKKL  IAGGTGITPVYQV QAI  D +D T
Sbjct:  664 VRKFRFALPSEDQVLGLPVGKHIFLCAVIDDKLCMRAYTPTSTIDEVGYFELVVKIYFKGIHPKFPNGGQMSQYLDSLQLGSFLDVKGPLGHIEYQGKGNFLVHGKQKFAKKLAMIAGGTGITPVYQVMQAILKDPEDDT 803

[Close]

BLAST of GR916070 vs. SwissProt
Match: NIA2_TOBAC (Nitrate reductase [NADH] 2 OS=Nicotiana tabacum GN=NIA2 PE=2 SV=2)

HSP 1 Score: 232.646 bits (592), Expect = 6.305e-61
Identity = 108/140 (77.14%), Postives = 120/140 (85.71%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VR FRFALP EDQ LGLP+GKHIFLC  ID KLCMRAYTPTS++DE+GYF+LVVKIYFK +HPKFPNGG MSQYLDS+ +GS LDVKGP+GHIEY G GNF+V GKQ+FAKKL  IAGGTGITPVYQV QAI  D +D T
Sbjct:  664 VRKFRFALPSEDQVLGLPVGKHIFLCAVIDDKLCMRAYTPTSTIDEVGYFELVVKIYFKGIHPKFPNGGQMSQYLDSMPLGSFLDVKGPLGHIEYQGKGNFLVHGKQKFAKKLAMIAGGTGITPVYQVMQAILKDPEDDT 803

[Close]

BLAST of GR916070 vs. SwissProt
Match: NIA2_PHAVU (Nitrate reductase [NADH] 2 OS=Phaseolus vulgaris GN=NIA2 PE=3 SV=1)

HSP 1 Score: 231.106 bits (588), Expect = 1.834e-60
Identity = 105/140 (75.00%), Postives = 124/140 (88.57%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VRLFRF +P ++Q LGLP+GKHIFLC  IDGKLCMRAYTPTSSV+E+G+FDL++K+YFK+VHPKFPNGGLMSQYL+SL+IGS+LDVKGP+GHIEY G GNF V GK RFAK+L  +AGGTGITP+YQVAQAI  D +D T
Sbjct:  651 VRLFRFEMPSKNQLLGLPVGKHIFLCATIDGKLCMRAYTPTSSVEEVGFFDLLIKVYFKDVHPKFPNGGLMSQYLESLSIGSMLDVKGPLGHIEYTGRGNFTVNGKSRFAKRLAMLAGGTGITPIYQVAQAILKDPEDLT 790

[Close]

BLAST of GR916070 vs. SwissProt
Match: NIA1_PHAVU (Nitrate reductase [NADH] 1 OS=Phaseolus vulgaris GN=NIA1 PE=3 SV=1)

HSP 1 Score: 228.409 bits (581), Expect = 1.189e-59
Identity = 103/140 (73.57%), Postives = 120/140 (85.71%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VRL RFALP EDQ +GLP+G H+FLC  +D KLCMRAYTPTSSVDE+G+FDLVVK+YFK VHP FPNGG+MSQ+LDSL IGS++DVKGP+GHIEY G GNF+V GK RFAK+LT +AGGTGITP+YQV QAI  D +D T
Sbjct:  642 VRLLRFALPAEDQVMGLPVGNHVFLCATVDEKLCMRAYTPTSSVDEVGFFDLVVKVYFKGVHPNFPNGGIMSQHLDSLPIGSVVDVKGPLGHIEYTGRGNFLVHGKPRFAKRLTMLAGGTGITPIYQVVQAILKDPEDRT 781

[Close]

BLAST of GR916070 vs. SwissProt
Match: NIA_SOLLC (Nitrate reductase [NADH] OS=Solanum lycopersicum GN=NIA PE=3 SV=1)

HSP 1 Score: 227.639 bits (579), Expect = 2.028e-59
Identity = 106/140 (75.71%), Postives = 120/140 (85.71%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VR F+FALP EDQ LGLP+GKHIFLC  +D KLCMRAYTPTS+VDE+G+F+LVVKIYFK VHPKFPNGG MSQ+LDSL IG+ LDVKGP+GHIEY G GNF+V GKQ+FAKKL  IAGGTGITPVYQV Q+I  D +D T
Sbjct:  671 VRKFKFALPSEDQVLGLPVGKHIFLCATVDDKLCMRAYTPTSTVDEVGFFELVVKIYFKGVHPKFPNGGQMSQHLDSLPIGAFLDVKGPLGHIEYQGKGNFLVHGKQKFAKKLAMIAGGTGITPVYQVMQSILKDPEDDT 810

[Close]

BLAST of GR916070 vs. SwissProt
Match: NIA_CUCMA (Nitrate reductase [NADH] OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 227.254 bits (578), Expect = 2.649e-59
Identity = 103/141 (73.05%), Postives = 122/141 (86.52%), Query Frame = 1

Query:   28 VRLFRFALPY-EDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VR+FRFALP  +DQ LGLP+GKHIF+C  +DGKLCMRAYTPTSS+DE+G+F+LVVK+YFK VHPKFPNGG+MSQYLDS+ +GS LDVKGP+GHIEY G GNFMV GK RFA++L  +AGGTGITP+YQV QAI  D +D T
Sbjct:  678 VRVFRFALPGGQDQALGLPVGKHIFICATVDGKLCMRAYTPTSSIDEMGFFELVVKVYFKGVHPKFPNGGIMSQYLDSMEVGSTLDVKGPLGHIEYTGRGNFMVHGKPRFARRLAMLAGGTGITPIYQVVQAILKDPEDET 818

[Close]

BLAST of GR916070 vs. SwissProt
Match: NIA_SPIOL (Nitrate reductase [NADH] OS=Spinacia oleracea GN=NIA PE=1 SV=1)

HSP 1 Score: 224.557 bits (571), Expect = 1.717e-58
Identity = 101/140 (72.14%), Postives = 121/140 (86.43%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VR FRF LP EDQ LGLP+GKHIFLC  +D KLCMRAYTP+S++D +GYFDLVVK+YFK+VHP+FPNGG+MSQ+LDSL++GSI+DVKGP+GHIEY+G GNF V GK +FAKKL  I+GGTGITP+YQV QAI  D +D T
Sbjct:  687 VRRFRFGLPSEDQVLGLPVGKHIFLCANVDDKLCMRAYTPSSTIDVVGYFDLVVKVYFKDVHPRFPNGGVMSQHLDSLSLGSIVDVKGPLGHIEYLGKGNFTVHGKPKFAKKLAMISGGTGITPIYQVMQAILKDPEDKT 826

[Close]

BLAST of GR916070 vs. SwissProt
Match: NIA_PETHY (Nitrate reductase [NADH] OS=Petunia hybrida GN=NIA PE=2 SV=1)

HSP 1 Score: 224.172 bits (570), Expect = 2.242e-58
Identity = 105/140 (75.00%), Postives = 119/140 (85.00%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VR FRFALP EDQ LGLP+GKHIFLC  ID KLCMRAYTPTS+VDE+GYF+LVVKIYFK + PKFPNGG MSQYLDSL +G+ +DVKGP+GHIEY G GNF+V GK++FAKKL  +AGGTGITPVYQV QAI  D +D T
Sbjct:  669 VRKFRFALPSEDQVLGLPVGKHIFLCAIIDDKLCMRAYTPTSTVDEVGYFELVVKIYFKGIVPKFPNGGQMSQYLDSLPLGAFVDVKGPLGHIEYQGRGNFLVHGKRKFAKKLAMLAGGTGITPVYQVMQAILKDPEDET 808

[Close]

BLAST of GR916070 vs. SwissProt
Match: NIA2_BRANA (Nitrate reductase [NADH], clone PBNBR1412 OS=Brassica napus GN=NIA2 PE=2 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 4.995e-58
Identity = 101/140 (72.14%), Postives = 122/140 (87.14%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VR FRFALP EDQQLGLP+GKHIFLC  I+ KLC+RAYTPTS+VD +G+ DLVVK+YFK+VHP+FPNGGLMSQ+LDSL IGS+L++KGP+GHIEY+G GNFMV GK +FAKKL  +AGG GITP+YQV Q+I +D +D T
Sbjct:  671 VRRFRFALPSEDQQLGLPVGKHIFLCANINDKLCLRAYTPTSTVDAVGHIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSVLNIKGPLGHIEYLGKGNFMVTGKPKFAKKLAMLAGGPGITPIYQVIQSILSDPEDET 810

[Close]

BLAST of GR916070 vs. TrEMBL
Match: Q9FUC2_RICCO (Nitrate reductase OS=Ricinus communis GN=NIA PE=2 SV=1)

HSP 1 Score: 233.417 bits (594), Expect = 5.637e-60
Identity = 106/140 (75.71%), Postives = 122/140 (87.14%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VRLFRFALP +DQ LGLP+GKHIFLC  ID KLCMRAYTPTS++D +GYFDLV+K+YFK VHPKFPNGGLMSQ+LDSL +GS++DVKGP+GHIEY G GNFMV GK +FAKKLT +AGGTGITP+YQV QAI  D +D T
Sbjct:  674 VRLFRFALPSDDQVLGLPVGKHIFLCATIDEKLCMRAYTPTSTIDVVGYFDLVIKVYFKGVHPKFPNGGLMSQHLDSLQLGSVIDVKGPLGHIEYAGRGNFMVHGKPKFAKKLTMLAGGTGITPIYQVIQAILKDPEDDT 813

[Close]

BLAST of GR916070 vs. TrEMBL
Match: B9RJB1_RICCO (Nitrate reductase, putative OS=Ricinus communis GN=RCOM_1032820 PE=4 SV=1)

HSP 1 Score: 233.417 bits (594), Expect = 5.637e-60
Identity = 106/140 (75.71%), Postives = 122/140 (87.14%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VRLFRFALP +DQ LGLP+GKHIFLC  ID KLCMRAYTPTS++D +GYFDLV+K+YFK VHPKFPNGGLMSQ+LDSL +GS++DVKGP+GHIEY G GNFMV GK +FAKKLT +AGGTGITP+YQV QAI  D +D T
Sbjct:  674 VRLFRFALPSDDQVLGLPVGKHIFLCATIDEKLCMRAYTPTSTIDVVGYFDLVIKVYFKGVHPKFPNGGLMSQHLDSLQLGSVIDVKGPLGHIEYAGRGNFMVHGKPKFAKKLTMLAGGTGITPIYQVIQAILKDPEDDT 813

[Close]

BLAST of GR916070 vs. TrEMBL
Match: Q93XS1_PRUPE (Nitrate reductase OS=Prunus persica GN=PpNR1 PE=2 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 7.362e-60
Identity = 106/140 (75.71%), Postives = 124/140 (88.57%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VRLFRFALP EDQ LGLP+GKHIF+C  I+GKLCMRAYTP+SS+DE+GYFDLVVKIYFKNVHPKFP+GGLMSQ+LDSL IG+ +D+KGP+GHIEY G G+F+V GK +FAKKL  +AGGTGITP+YQVAQAI  D +D T
Sbjct:  668 VRLFRFALPSEDQVLGLPVGKHIFVCATIEGKLCMRAYTPSSSIDEVGYFDLVVKIYFKNVHPKFPDGGLMSQHLDSLPIGAAIDIKGPLGHIEYTGRGHFLVNGKPKFAKKLAMLAGGTGITPIYQVAQAILKDPEDET 807

[Close]

BLAST of GR916070 vs. TrEMBL
Match: Q43461_SOYBN (Nitrate reductase (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 9.615e-60
Identity = 106/140 (75.71%), Postives = 122/140 (87.14%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VRLFR  LP +DQ LGLP+GKHIFLC  I+ KLCMRAYTPTSSVDE+G+FDL +K+YFK VHPKFP GGLMSQ+LDSL+IGS+LDVKGP+GHIEY G GNF+V GKQRFAK+L  +AGGTGITP+YQVAQAI  D +DPT
Sbjct:   42 VRLFRLGLPSKDQLLGLPVGKHIFLCATINEKLCMRAYTPTSSVDEVGFFDLAIKVYFKGVHPKFPRGGLMSQHLDSLSIGSVLDVKGPLGHIEYTGRGNFLVHGKQRFAKRLAMLAGGTGITPIYQVAQAILKDPEDPT 181

[Close]

BLAST of GR916070 vs. TrEMBL
Match: Q8H1T7_TILPL (Nitrate reductase OS=Tilia platyphyllos GN=nr1 PE=2 SV=1)

HSP 1 Score: 231.876 bits (590), Expect = 1.640e-59
Identity = 107/140 (76.43%), Postives = 121/140 (86.43%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VRLFRFALP +DQ LGLP+GKHIFLCV ID KLCMRAYTPTS++DE+ +FDLVVK+YFK VHPKFPNGGLMSQYLDSL +GS+LDVKGP+GHIEY G GNF V GK +FAKKL  +AGGTGITP+YQV QAI  D +D T
Sbjct:  655 VRLFRFALPSDDQVLGLPVGKHIFLCVTIDDKLCMRAYTPTSTIDEVDHFDLVVKVYFKGVHPKFPNGGLMSQYLDSLPLGSLLDVKGPLGHIEYTGRGNFSVHGKPKFAKKLAMLAGGTGITPIYQVIQAILKDPEDET 794

[Close]

BLAST of GR916070 vs. TrEMBL
Match: Q18NX4_NICBE (Nitrate reductase 2 OS=Nicotiana benthamiana GN=NR2 PE=2 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 2.142e-59
Identity = 106/140 (75.71%), Postives = 120/140 (85.71%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VR FRFALP EDQ LGLP+GKHIFLC  ID KLCMRAYTPTS++DE+GYF+LVVKIYFK +HPKFPNGG MSQYLDS+ +GS LDVKGP+GHIEY G GNF+V GKQ+FAKKL  +AGGTGITP+YQV QAI  D +D T
Sbjct:  664 VRKFRFALPSEDQVLGLPVGKHIFLCAVIDDKLCMRAYTPTSTIDEVGYFELVVKIYFKGIHPKFPNGGQMSQYLDSMPLGSFLDVKGPLGHIEYQGKGNFLVHGKQKFAKKLAMLAGGTGITPIYQVMQAILKDPEDDT 803

[Close]

BLAST of GR916070 vs. TrEMBL
Match: D6CIN9_SAPOF (Putative nitrate reductase (NADH) (Fragment) OS=Saponaria officinalis GN=nia1 PE=4 SV=1)

HSP 1 Score: 231.106 bits (588), Expect = 2.798e-59
Identity = 105/140 (75.00%), Postives = 121/140 (86.43%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VRLFRFALP EDQ LGLP+GKHI+LC  ID KLCMRAYTPT+++DE+G+FDLV+K+YFK  HPKFPNGGLMSQYLDSL +GS LDVKGP+GHIEYIG GNF+V GK +FAKKL  +AGGTGITP+YQV QAI  D +D T
Sbjct:  616 VRLFRFALPLEDQVLGLPVGKHIYLCAIIDDKLCMRAYTPTTNIDEVGHFDLVIKVYFKGEHPKFPNGGLMSQYLDSLALGSSLDVKGPLGHIEYIGRGNFLVSGKPKFAKKLAMLAGGTGITPIYQVIQAILKDPEDET 755

[Close]

BLAST of GR916070 vs. TrEMBL
Match: Q3C1F0_NICBE (Nitrate reductase OS=Nicotiana benthamiana GN=NR PE=2 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 4.772e-59
Identity = 106/140 (75.71%), Postives = 120/140 (85.71%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VR FRFALP EDQ LGLP+GKHIFLC  ID KLCMRAYTPTS++DE+GYF+LVVKIYFK +HPKFPNGG MSQYLDS+ +GS L+VKGP+GHIEY G GNF+V GKQ+FAKKL  IAGGTGITP+YQV QAI  D +D T
Sbjct:  664 VRKFRFALPSEDQVLGLPVGKHIFLCAVIDDKLCMRAYTPTSTIDEVGYFELVVKIYFKGIHPKFPNGGQMSQYLDSMPLGSFLNVKGPLGHIEYQGKGNFLVHGKQKFAKKLAMIAGGTGITPIYQVMQAILKDPEDDT 803

[Close]

BLAST of GR916070 vs. TrEMBL
Match: Q8LT13_SOLTU (Nitrate reductase OS=Solanum tuberosum GN=StNR6 PE=2 SV=1)

HSP 1 Score: 227.639 bits (579), Expect = 3.093e-58
Identity = 106/140 (75.71%), Postives = 120/140 (85.71%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VR F+FALP EDQ LGLP+GKHIFLC  +D KLCMRAYTPTS+VDE+G+F+LVVKIYFK VHPKFPNGG MSQ+LDSL IG+ LDVKGP+GHIEY G GNF+V GKQ+FAKKL  IAGGTGITPVYQV Q+I  D +D T
Sbjct:  671 VRKFKFALPSEDQVLGLPVGKHIFLCATVDDKLCMRAYTPTSTVDEVGFFELVVKIYFKGVHPKFPNGGQMSQHLDSLPIGAFLDVKGPLGHIEYQGKGNFLVHGKQKFAKKLAMIAGGTGITPVYQVMQSILKDPEDDT 810

[Close]

BLAST of GR916070 vs. TrEMBL
Match: Q9SYR0_SOYBN (Nitrate reductase (Fragment) OS=Glycine max GN=nr2 PE=4 SV=1)

HSP 1 Score: 226.483 bits (576), Expect = 6.891e-58
Identity = 104/140 (74.29%), Postives = 119/140 (85.00%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VRLFRFALP +D  +GLP+GKHIFLC  +D KLCMRAYTPTSSV E+GYFDLVVK+YFK VHPKFPNGG+MSQ+LDSL IGS+LDVKGP+GHIEY G GNF+V GK RFA +L  +AGGTGITP+YQV QAI  D +D T
Sbjct:  651 VRLFRFALPSDDLLMGLPVGKHIFLCATVDEKLCMRAYTPTSSVHEVGYFDLVVKVYFKGVHPKFPNGGIMSQHLDSLPIGSVLDVKGPLGHIEYTGRGNFLVHGKPRFATRLAMLAGGTGITPIYQVVQAILKDPEDCT 790

[Close]

BLAST of GR916070 vs. TAIR peptide
Match: AT1G77760.1 (| Symbols: NIA1, GNR1, NR1 | nitrate reductase 1 | chr1:29236005-29239367 REVERSE LENGTH=917)

HSP 1 Score: 220.705 bits (561), Expect = 2.861e-58
Identity = 97/140 (69.29%), Postives = 122/140 (87.14%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VR FRFALP EDQQLGLP+GKH+F+C  I+ KLC+RAYTPTS++D +G+ DLVVK+YFK+VHP+FPNGGLMSQ+LDSL IGS++D+KGP+GHIEY G GNF+V GK +FAKKL  +AGGTGITP+YQ+ Q+I +D +D T
Sbjct:  677 VRKFRFALPSEDQQLGLPVGKHVFVCANINDKLCLRAYTPTSAIDAVGHIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIEYKGKGNFLVSGKPKFAKKLAMLAGGTGITPIYQIIQSILSDPEDET 816

[Close]

BLAST of GR916070 vs. TAIR peptide
Match: AT1G37130.1 (| Symbols: NIA2, B29, NIA2-1, CHL3, NR, NR2, ATNR2 | nitrate reductase 2 | chr1:14158617-14161652 FORWARD LENGTH=917)

HSP 1 Score: 214.927 bits (546), Expect = 1.570e-56
Identity = 98/140 (70.00%), Postives = 116/140 (82.86%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            VR FRFALP ED  LGLP+GKHIFLC  I+ KLC+RAYTP+S+VD +GYF+LVVKIYF  VHP+FPNGGLMSQYLDSL IGS L++KGP+GH+EY+G G+F V GK +FA KL  +AGGTGITPVYQ+ QAI  D +D T
Sbjct:  677 VRKFRFALPVEDMVLGLPVGKHIFLCATINDKLCLRAYTPSSTVDVVGYFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEYLGKGSFTVHGKPKFADKLAMLAGGTGITPVYQIIQAILKDPEDET 816

[Close]

BLAST of GR916070 vs. TAIR peptide
Match: AT5G17770.1 (| Symbols: ATCBR, CBR1, CBR | NADH:cytochrome B5 reductase 1 | chr5:5864543-5866495 REVERSE LENGTH=281)

HSP 1 Score: 79.337 bits (194), Expect = 1.029e-15
Identity = 54/142 (38.03%), Postives = 75/142 (52.82%), Query Frame = 1

Query:   28 VRLFRFALPYEDQQLGLPIGKHIFLCVAIDGKL--CMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            V  F F LP     LGLPIG+HI  C   DG+    ++ YTPT+   ++G F+LV+K+Y +         G MS +   + +G  L VKGP G  +Y   G F   G          +AGG+GITP++QVA+AI  +  D T
Sbjct:   62 VAKFVFELPTSTSVLGLPIGQHIS-CRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQ---------GRMSHHFREMRVGDHLAVKGPKGRFKY-QPGQFRAFG---------MLAGGSGITPMFQVARAILENPTDKT 183

[Close]

BLAST of GR916070 vs. TAIR peptide
Match: AT5G20080.1 (| Symbols: | FAD/NAD(P)-binding oxidoreductase | chr5:6782708-6786360 FORWARD LENGTH=328)

HSP 1 Score: 70.0922 bits (170), Expect = 6.245e-13
Identity = 49/149 (32.89%), Postives = 69/149 (46.31%), Query Frame = 1

Query:   31 RLFRFALPYEDQQLGLPIGKHIFLCVAIDG----------KLCMRAYTPTSSVDEIGYFDLVVKIYFKNVHPKFPNGGLMSQYLDSLTIGSILDVKGPVGHIEYIGSGNFMVQGKQRFAKKLTFIAGGTGITPVYQVAQAIFNDSDDPT 447
            +LFRF+     +     +G H+  C+              K  +R YTP S  +  GYFDL++K+Y           G MSQ+  SL  G +L+VKGPV   +Y               K +  IAGG+GITP+ QV  AI  + +D T
Sbjct:   94 QLFRFSFDPSAE-----LGLHVASCLLTRAPLGYNAEGKTKYVIRPYTPISDPEAKGYFDLLIKVY---------PDGKMSQHFASLKPGDVLEVKGPVEKFKY----------SPNMKKHIGMIAGGSGITPMLQVIDAIVKNPEDNT 218

The following BLAST results are available for this feature:

BLAST of GR916070 vs. SwissProt
BLAST of GR916070 vs. TrEMBL
BLAST of GR916070 vs. TAIR peptide

BLAST of GR916070 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
NIA_LOTJA	1.960e-62	78.57	Nitrate reductase [NADH] OS=Lotus japonicus GN=NIA...	[more]
NIA1_TOBAC	3.696e-61	77.86	Nitrate reductase [NADH] 1 OS=Nicotiana tabacum GN...	[more]
NIA2_TOBAC	6.305e-61	77.14	Nitrate reductase [NADH] 2 OS=Nicotiana tabacum GN...	[more]
NIA2_PHAVU	1.834e-60	75.00	Nitrate reductase [NADH] 2 OS=Phaseolus vulgaris G...	[more]
NIA1_PHAVU	1.189e-59	73.57	Nitrate reductase [NADH] 1 OS=Phaseolus vulgaris G...	[more]
NIA_SOLLC	2.028e-59	75.71	Nitrate reductase [NADH] OS=Solanum lycopersicum G...	[more]
NIA_CUCMA	2.649e-59	73.05	Nitrate reductase [NADH] OS=Cucurbita maxima PE=2 ...	[more]
NIA_SPIOL	1.717e-58	72.14	Nitrate reductase [NADH] OS=Spinacia oleracea GN=N...	[more]
NIA_PETHY	2.242e-58	75.00	Nitrate reductase [NADH] OS=Petunia hybrida GN=NIA...	[more]
NIA2_BRANA	4.995e-58	72.14	Nitrate reductase [NADH], clone PBNBR1412 OS=Brass...	[more]

BLAST of GR916070 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9FUC2_RICCO	5.637e-60	75.71	Nitrate reductase OS=Ricinus communis GN=NIA PE=2 ...	[more]
B9RJB1_RICCO	5.637e-60	75.71	Nitrate reductase, putative OS=Ricinus communis GN...	[more]
Q93XS1_PRUPE	7.362e-60	75.71	Nitrate reductase OS=Prunus persica GN=PpNR1 PE=2 ...	[more]
Q43461_SOYBN	9.615e-60	75.71	Nitrate reductase (Fragment) OS=Glycine max PE=2 S...	[more]
Q8H1T7_TILPL	1.640e-59	76.43	Nitrate reductase OS=Tilia platyphyllos GN=nr1 PE=...	[more]
Q18NX4_NICBE	2.142e-59	75.71	Nitrate reductase 2 OS=Nicotiana benthamiana GN=NR...	[more]
D6CIN9_SAPOF	2.798e-59	75.00	Putative nitrate reductase (NADH) (Fragment) OS=Sa...	[more]
Q3C1F0_NICBE	4.772e-59	75.71	Nitrate reductase OS=Nicotiana benthamiana GN=NR P...	[more]
Q8LT13_SOLTU	3.093e-58	75.71	Nitrate reductase OS=Solanum tuberosum GN=StNR6 PE...	[more]
Q9SYR0_SOYBN	6.891e-58	74.29	Nitrate reductase (Fragment) OS=Glycine max GN=nr2...	[more]

BLAST of GR916070 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT1G77760.1	2.861e-58	69.29	\| Symbols: NIA1, GNR1, NR1 \| nitrate reductase 1 \|...	[more]
AT1G37130.1	1.570e-56	70.00	\| Symbols: NIA2, B29, NIA2-1, CHL3, NR, NR2, ATNR2...	[more]
AT5G17770.1	1.029e-15	38.03	\| Symbols: ATCBR, CBR1, CBR \| NADH:cytochrome B5 r...	[more]
AT5G20080.1	6.245e-13	32.89	\| Symbols: \| FAD/NAD(P)-binding oxidoreductase \| ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001433	Oxidoreductase FAD/NAD(P)-binding	PFAM	PF00175	NAD_binding_1	coord: 124..149 score: 1.3
IPR001709	Flavoprotein pyridine nucleotide cytochrome reductase	PRINTS	PR00371	FPNCR	coord: 45..52 score: 4.3e-06coord: 77..86 score: 4.3e-06coord: 123..142 score: 4.3
IPR001834	NADH:cytochrome b5 reductase (CBR)	PRINTS	PR00406	CYTB5RDTASE	coord: 24..35 score: 9.7e-29coord: 45..52 score: 9.7e-29coord: 90..104 score: 9.7e-29coord: 123..142 score: 9.7
IPR008333	Oxidoreductase, FAD-binding domain	PFAM	PF00970	FAD_binding_6	coord: 10..104 score: 1.2
IPR017927	Ferredoxin reductase-type FAD-binding domain	PROFILE	PS51384	FAD_FR	coord: 1..105 score: 12
IPR017938	Riboflavin synthase-like beta-barrel	SUPERFAMILY	63380	Riboflavin synthase domain-like	coord: 10..107 score: 2.4
None	No IPR available	GENE3D	2.40.30.10	no description	coord: 10..91 score: 1.3
None	No IPR available	GENE3D	3.40.50.80	no description	coord: 92..149 score: 1.4
None	No IPR available	PANTHER	PTHR19370	NADH-CYTOCHROME B5 REDUCTASE	coord: 10..128 score: 6.7
None	No IPR available	PANTHER	PTHR19370:SF5	NITRATE REDUCTASE	coord: 10..128 score: 6.7
None	No IPR available	SUPERFAMILY	52343	Ferredoxin reductase-like, C-terminal NADP-linked domain	coord: 92..150 score: 1.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR916070 ID=GR916070; Name=GR916070; organism=Cicer arietinum; type=EST; length=465bp
GATTTCGAGCGGCCGCCCGGGCAGGTTGTAAGACTCTTCCGTTTTGCATT
ACCTTATGAAGACCAACAATTGGGATTACCCATTGGGAAACACATTTTCT
TATGTGTTGCCATAGATGGGAAACTATGCATGCGAGCTTATACTCCAACG
AGTAGTGTCGATGAAATTGGGTATTTTGATTTAGTTGTCAAAATTTACTT
CAAAAACGTGCACCCTAAGTTTCCTAATGGTGGACTTATGTCACAATATT
TGGACTCACTTACTATTGGGTCAATATTGGACGTAAAAGGTCCAGTGGGT
CACATTGAGTATATTGGAAGTGGAAACTTCATGGTTCAGGGGAAACAAAG
GTTTGCGAAGAAACTAACTTTTATAGCTGGTGGGACCGGGATCACACCTG
TTTATCAAGTGGCGCAAGCGATATTCAATGACAGCGATGACCCTACCTCG
GCCGCGACCACGCTA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR001433	OxRdtase_FAD/NAD-bd
IPR001709	Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR001834	CBR-like
IPR008333	Cbr1-like_FAD-bd_dom
IPR017927	FAD-bd_FR_type
IPR017938	Riboflavin_synthase-like_b-brl

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