GR407247

EST Overview
NameGR407247
Unique NameGR407247
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length615
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Homology
[Close]
BLAST of GR407247 vs. SwissProt
Match: M2K5_ARATH (Mitogen-activated protein kinase kinase 5 OS=Arabidopsis thaliana GN=MKK5 PE=1 SV=2)

HSP 1 Score: 85.1149 bits (209), Expect = 3.053e-16
Identity = 37/57 (64.91%), Postives = 44/57 (77.19%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFLVRTVSNHN 172
            SLMCAICMSQPPEAP +AS EFR FVS CLQ DP +RW+A +LL HPF+++     N
Sbjct:  277 SLMCAICMSQPPEAPATASQEFRHFVSCCLQSDPPKRWSAQQLLQHPFILKATGGPN 333
[Close]
BLAST of GR407247 vs. SwissProt
Match: M2K4_ARATH (Mitogen-activated protein kinase kinase 4 OS=Arabidopsis thaliana GN=MKK4 PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 5.757e-15
Identity = 36/58 (62.07%), Postives = 46/58 (79.31%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFLVRTVSNHNQ 175
            SLMCAICMSQPPEAP +AS EFR F+S CLQR+P +R +A +LL HPF++R   + N+
Sbjct:  286 SLMCAICMSQPPEAPATASPEFRHFISCCLQREPGKRRSAMQLLQHPFILRASPSQNR 343
[Close]
BLAST of GR407247 vs. TrEMBL
Match: Q93WR8_MEDVA (MEK map kinase kinsae OS=Medicago varia GN=simkk PE=2 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 3.957e-24
Identity = 54/58 (93.10%), Postives = 55/58 (94.83%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFLVRTVSNHNQ 175
            SLMCAICMSQPPEAPT+AS EFRDFVSRCLQRDPSRRWTASRLLSHPFLVR  SNHNQ
Sbjct:  293 SLMCAICMSQPPEAPTTASPEFRDFVSRCLQRDPSRRWTASRLLSHPFLVRNGSNHNQ 350
[Close]
BLAST of GR407247 vs. TrEMBL
Match: B9HK72_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_563479 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 4.685e-17
Identity = 40/51 (78.43%), Postives = 47/51 (92.16%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFLVR 154
            SLMCAICMSQPPEAP SAS EFR+F++ CLQR+PSRRWTA++LL HPF+VR
Sbjct:  280 SLMCAICMSQPPEAPASASREFRNFIACCLQREPSRRWTANQLLQHPFIVR 330
[Close]
BLAST of GR407247 vs. TrEMBL
Match: D7T442_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_18.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00016115001 PE=4 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.363e-16
Identity = 39/58 (67.24%), Postives = 48/58 (82.76%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFLVRTVSNHNQ 175
            SLMCAICMSQPPEAP +AS EFRDF+SRCLQRDP+ RW+A +LL HPF++++     Q
Sbjct:  265 SLMCAICMSQPPEAPVTASREFRDFISRCLQRDPAVRWSADKLLRHPFVLQSQRRRGQ 322
[Close]
BLAST of GR407247 vs. TrEMBL
Match: Q6QMT4_PETCR (Mitogen-activated protein kinase kinase 5 OS=Petroselinum crispum GN=MKK5 PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.780e-16
Identity = 39/52 (75.00%), Postives = 47/52 (90.38%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFLVRT 157
            SLMCAICMSQPPEAP +AS +FR+F+S CLQRDP RRWTA++LL HPF+V+T
Sbjct:  273 SLMCAICMSQPPEAPPTASRQFREFISCCLQRDPHRRWTANQLLRHPFVVQT 324
[Close]
BLAST of GR407247 vs. TrEMBL
Match: Q66MH8_SOLLC (MAPKK OS=Solanum lycopersicum GN=LeMKK2 PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 5.180e-16
Identity = 38/51 (74.51%), Postives = 45/51 (88.24%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFLVR 154
            SLMCAICMSQPPEAP SAS EFR+F++ CLQRDP+RRWTA +LL HPF+ +
Sbjct:  277 SLMCAICMSQPPEAPPSASREFREFIACCLQRDPARRWTAGQLLRHPFITQ 327
[Close]
BLAST of GR407247 vs. TrEMBL
Match: Q14TB0_TOBAC (Mitogen-activated protein kinase 2 OS=Nicotiana tabacum GN=NtMEK2 PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 5.180e-16
Identity = 38/51 (74.51%), Postives = 45/51 (88.24%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFLVR 154
            SLMCAICMSQPPEAP +AS EFRDF++ CLQRDP+RRWTA +LL HPF+ +
Sbjct:  289 SLMCAICMSQPPEAPANASREFRDFIACCLQRDPARRWTAVQLLRHPFITQ 339
[Close]
BLAST of GR407247 vs. TrEMBL
Match: B2NIC3_NICBE (MAP kinase kinase OS=Nicotiana benthamiana GN=NbMEK2 PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 5.180e-16
Identity = 38/51 (74.51%), Postives = 45/51 (88.24%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFLVR 154
            SLMCAICMSQPPEAP +AS EFRDF++ CLQRDP+RRWTA +LL HPF+ +
Sbjct:  289 SLMCAICMSQPPEAPANASREFRDFIACCLQRDPARRWTAVQLLRHPFITQ 339
[Close]
BLAST of GR407247 vs. TrEMBL
Match: Q7XAC9_SOLTU (Mitogen-activated protein kinase kinase OS=Solanum tuberosum GN=StMEK2 PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 8.835e-16
Identity = 37/51 (72.55%), Postives = 46/51 (90.20%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFLVR 154
            SLMCAICMSQPPEAP +AS EFR+F++ CLQRDP+RRWTA++LL HPF+ +
Sbjct:  290 SLMCAICMSQPPEAPPTASREFREFIACCLQRDPARRWTAAQLLRHPFITQ 340
[Close]
BLAST of GR407247 vs. TrEMBL
Match: B9HVE0_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_228518 PE=4 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 8.835e-16
Identity = 38/51 (74.51%), Postives = 47/51 (92.16%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFLVR 154
            SLMCAICMSQPPEAP +AS EFRDF++ CLQR+P+RR+TA++LL HPF+VR
Sbjct:  278 SLMCAICMSQPPEAPATASREFRDFIACCLQREPARRFTANQLLQHPFIVR 328
[Close]
BLAST of GR407247 vs. TrEMBL
Match: D7L0Q4_ARALY (ATMKK5 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_479642 PE=4 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 7.479e-15
Identity = 37/57 (64.91%), Postives = 44/57 (77.19%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFLVRTVSNHN 172
            SLMCAICMSQPPEAP +AS EFR FVS CLQ DP +RW+A +LL HPF+++     N
Sbjct:  278 SLMCAICMSQPPEAPVTASQEFRHFVSCCLQSDPPKRWSAQQLLQHPFILKASGGPN 334
[Close]
BLAST of GR407247 vs. TAIR peptide
Match: AT3G21220.1 (| Symbols: ATMKK5, ATMAP2K_ALPHA, MAP2K_A, MKK5, ATMEK5, MEK5 | MAP kinase kinase 5 | chr3:7445917-7446963 FORWARD LENGTH=348)

HSP 1 Score: 85.1149 bits (209), Expect = 3.175e-17
Identity = 37/57 (64.91%), Postives = 44/57 (77.19%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFLVRTVSNHN 172
            SLMCAICMSQPPEAP +AS EFR FVS CLQ DP +RW+A +LL HPF+++     N
Sbjct:  277 SLMCAICMSQPPEAPATASQEFRHFVSCCLQSDPPKRWSAQQLLQHPFILKATGGPN 333
[Close]
BLAST of GR407247 vs. TAIR peptide
Match: AT1G51660.1 (| Symbols: ATMKK4, MKK4, ATMEK4 | mitogen-activated protein kinase kinase 4 | chr1:19154575-19155675 FORWARD LENGTH=366)

HSP 1 Score: 80.8777 bits (198), Expect = 5.989e-16
Identity = 36/58 (62.07%), Postives = 46/58 (79.31%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFLVRTVSNHNQ 175
            SLMCAICMSQPPEAP +AS EFR F+S CLQR+P +R +A +LL HPF++R   + N+
Sbjct:  286 SLMCAICMSQPPEAPATASPEFRHFISCCLQREPGKRRSAMQLLQHPFILRASPSQNR 343
[Close]
BLAST of GR407247 vs. TAIR peptide
Match: AT1G73500.1 (| Symbols: ATMKK9, MKK9 | MAP kinase kinase 9 | chr1:27639419-27640351 REVERSE LENGTH=310)

HSP 1 Score: 70.8626 bits (172), Expect = 6.197e-13
Identity = 29/49 (59.18%), Postives = 37/49 (75.51%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFL 148
            +LMCA+C  +PP AP   S EFR FV  CL++D S+RWTA +LL+HPFL
Sbjct:  258 TLMCAVCFGEPPRAPEGCSEEFRSFVECCLRKDSSKRWTAPQLLAHPFL 306
[Close]
BLAST of GR407247 vs. TAIR peptide
Match: AT1G18350.1 (| Symbols: ATMKK7, BUD1, MKK7 | MAP kinase kinase 7 | chr1:6315686-6316609 FORWARD LENGTH=307)

HSP 1 Score: 68.1662 bits (165), Expect = 4.017e-12
Identity = 28/49 (57.14%), Postives = 35/49 (71.43%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFL 148
            +LMC +C  +PP AP   S EFR FV  CL+++ S RWTAS+LL HPFL
Sbjct:  255 TLMCVVCFGEPPRAPEGCSDEFRSFVDCCLRKESSERWTASQLLGHPFL 303
[Close]
BLAST of GR407247 vs. TAIR peptide
Match: AT1G32320.1 (| Symbols: ATMKK10, MKK10 | MAP kinase kinase 10 | chr1:11655156-11656073 FORWARD LENGTH=305)

HSP 1 Score: 55.4546 bits (132), Expect = 2.694e-8
Identity = 24/49 (48.98%), Postives = 33/49 (67.35%), Query Frame = 2 
Query:    2 SLMCAICMSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFL 148
            +L CAIC ++  + P S S+EFRDFV RCL++D  +R T   LL H F+
Sbjct:  254 TLFCAICCNEKVDIPVSCSLEFRDFVGRCLEKDWRKRDTVEELLRHSFV 302
[Close]
BLAST of GR407247 vs. TAIR peptide
Match: AT1G05100.1 (| Symbols: MAPKKK18 | mitogen-activated protein kinase kinase kinase 18 | chr1:1469679-1470698 FORWARD LENGTH=339)

HSP 1 Score: 51.9878 bits (123), Expect = 2.979e-7
Identity = 20/43 (46.51%), Postives = 33/43 (76.74%), Query Frame = 2 
Query:   23 MSQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFLV 151
            + + PE P S + + +DF+ +CL+++ + RWTAS+LL+HPFLV
Sbjct:  222 LGELPELPCSLTEQAKDFLGKCLKKEATERWTASQLLNHPFLV 264
[Close]
BLAST of GR407247 vs. TAIR peptide
Match: AT2G32510.1 (| Symbols: MAPKKK17 | mitogen-activated protein kinase kinase kinase 17 | chr2:13798821-13799939 REVERSE LENGTH=372)

HSP 1 Score: 50.8322 bits (120), Expect = 6.636e-7
Identity = 20/41 (48.78%), Postives = 31/41 (75.61%), Query Frame = 2 
Query:   26 SQPPEAPTSASMEFRDFVSRCLQRDPSRRWTASRLLSHPFL 148
            S+ PE P   + E +DF+ +CL+R+ + RWTA++LL+HPFL
Sbjct:  219 SETPELPCLLAEEAKDFLEKCLKREANERWTATQLLNHPFL 259
The following BLAST results are available for this feature:
BLAST of GR407247 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 2
Match NameE-valueIdentityDescription
M2K5_ARATH3.053e-1664.91Mitogen-activated protein kinase kinase 5 OS=Arabi... [more]
M2K4_ARATH5.757e-1562.07Mitogen-activated protein kinase kinase 4 OS=Arabi... [more]
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BLAST of GR407247 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q93WR8_MEDVA3.957e-2493.10MEK map kinase kinsae OS=Medicago varia GN=simkk P... [more]
B9HK72_POPTR4.685e-1778.43Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
D7T442_VITVI1.363e-1667.24Whole genome shotgun sequence of line PN40024, sca... [more]
Q6QMT4_PETCR1.780e-1675.00Mitogen-activated protein kinase kinase 5 OS=Petro... [more]
Q66MH8_SOLLC5.180e-1674.51MAPKK OS=Solanum lycopersicum GN=LeMKK2 PE=2 SV=1[more]
Q14TB0_TOBAC5.180e-1674.51Mitogen-activated protein kinase 2 OS=Nicotiana ta... [more]
B2NIC3_NICBE5.180e-1674.51MAP kinase kinase OS=Nicotiana benthamiana GN=NbME... [more]
Q7XAC9_SOLTU8.835e-1672.55Mitogen-activated protein kinase kinase OS=Solanum... [more]
B9HVE0_POPTR8.835e-1674.51Predicted protein (Fragment) OS=Populus trichocarp... [more]
D7L0Q4_ARALY7.479e-1564.91ATMKK5 OS=Arabidopsis lyrata subsp. lyrata GN=ARAL... [more]
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BLAST of GR407247 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 7
Match NameE-valueIdentityDescription
AT3G21220.13.175e-1764.91| Symbols: ATMKK5, ATMAP2K_ALPHA, MAP2K_A, MKK5, A... [more]
AT1G51660.15.989e-1662.07| Symbols: ATMKK4, MKK4, ATMEK4 | mitogen-activate... [more]
AT1G73500.16.197e-1359.18| Symbols: ATMKK9, MKK9 | MAP kinase kinase 9 | ch... [more]
AT1G18350.14.017e-1257.14| Symbols: ATMKK7, BUD1, MKK7 | MAP kinase kinase ... [more]
AT1G32320.12.694e-848.98| Symbols: ATMKK10, MKK10 | MAP kinase kinase 10 |... [more]
AT1G05100.12.979e-746.51| Symbols: MAPKKK18 | mitogen-activated protein ki... [more]
AT2G32510.16.636e-748.78| Symbols: MAPKKK17 | mitogen-activated protein ki... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase, catalytic domainPROFILEPS50011PROTEIN_KINASE_DOMcoord: 1..49
score: 8
IPR011009Protein kinase-like domainSUPERFAMILY56112Protein kinase-like (PK-like)coord: 1..76
score: 1.1
IPR017442Serine/threonine-protein kinase-like domainPFAMPF00069Pkinasecoord: 16..49
score: 2.5
NoneNo IPR availableGENE3D1.10.510.10no descriptioncoord: 8..60
score: 3.2
NoneNo IPR availablePANTHERPTHR22986MAPKK-RELATED SERINE/THREONINE PROTEIN KINASEScoord: 1..76
score: 1.6
NoneNo IPR availablePANTHERPTHR22986:SF31MITOGEN ACTIVATED PROTEIN KINASE KINASEcoord: 1..76
score: 1.6
NoneNo IPR availableSEGsegsegcoord: 60..74
score
NoneNo IPR availableSEGsegsegcoord: 50..62
score
NoneNo IPR availableSEGsegsegcoord: 1..12
score
NoneNo IPR availableSEGsegsegcoord: 27..45
score

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GR407247 ID=GR407247; Name=GR407247; organism=Cicer arietinum; type=EST; length=615bp
GAGTTTGATGTGTGCGATTTGTATGTCGCAACCACCAGAAGCTCCAACGA
GTGCTTCGATGGAGTTTAGGGATTTCGTTTCAAGGTGTTTGCAGAGAGAT
CCATCGAGGAGATGGACTGCTTCCAGGTTGCTTTCGCATCCTTTTCTTGT
TAGGACGGTTTCAAATCATAATCAGATTCCTCCAAATCTTCATCAACTAC
TTCCTCCTCCACCAAGGCCTCTTTCTTCTTAATTATTATCTATGAAGCAC
CGATACTCGCAAGGACACCAGATACGACAATGATAATCGGCAATAACTGA
GAAAATTGAATTGATTGAATGTAATCAAGTGTCGGTCACGTGTCGGAAAT
CGGAAATCACGACCTATCTTTGATTAGAAGTATCGGCGCTACATGGATTG
TTATTATTAGTAGCTGTAATTTTTTTTTAATTATTACTACTTTGAAAAAA
AGAAGAACTGAATTGGTGAGATTAGATGTTGATTTGATCTTTTCTTCATC
ACCGTAACATGAATCTCTAGCATTGGCTTGCACTTGCTATGGCCATGGCC
AACTCTTTTATTTTTATTTTTCCTATTTTTAATTATCTTTTATAAATGTT
GTTATGTATCAAAAA
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004672protein kinase activity
GO:0005524ATP binding
Vocabulary: Biological Process
TermDefinition
GO:0006468protein phosphorylation
Vocabulary: INTERPRO
TermDefinition
IPR000719Prot_kinase_dom
IPR011009Kinase-like_dom_sf
IPR017442Serine/threonine-protein kinase-like domain