HO664264

EST Overview


Name	HO664264
Unique Name	HO664264
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	385

Libraries


Library Name	Type
LIBEST_026619 TDZ treated Chickpea SSH library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO664264 vs. SwissProt
Match: XCP1_ARATH (Xylem cysteine proteinase 1 OS=Arabidopsis thaliana GN=XCP1 PE=1 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 3.752e-34
Identity = 66/113 (58.41%), Postives = 88/113 (77.88%), Query Frame = 2

Query:   38 LAMAFISFSSKTLVLACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            +A +  S S  +L++A S    +  AFARDFSIVGY+ E L + DKL+ELFESWMS H K Y+++EEK+ RFEVF++NL HID+RN  +++YWLGLNEFADL+H+EFK +YLG
Sbjct:    1 MAFSAPSLSKFSLLVAISASALLCCAFARDFSIVGYTPEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEEFKGRYLG 113

[Close]

BLAST of HO664264 vs. SwissProt
Match: XCP2_ARATH (Xylem cysteine proteinase 2 OS=Arabidopsis thaliana GN=XCP2 PE=1 SV=2)

HSP 1 Score: 141.354 bits (355), Expect = 1.426e-33
Identity = 72/111 (64.86%), Postives = 84/111 (75.68%), Query Frame = 2

Query:   53 ISFSSKTLVLACSLC---LFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            +S  S+ L  A +L    L +S A + D+SIVGYS EDL+S DKLIELFE+W+S   K YET+EEK LRFEVFKDNLKHIDE NK   +YWLGLNEFADLSH+EFK  YLG
Sbjct:    3 LSSPSRILCFALALSAASLSLSFASSHDYSIVGYSPEDLESHDKLIELFENWISNFEKAYETVEEKFLRFEVFKDNLKHIDETNKKGKSYWLGLNEFADLSHEEFKKMYLG 113

[Close]

BLAST of HO664264 vs. SwissProt
Match: PAPA4_CARPA (Papaya proteinase 4 OS=Carica papaya PE=1 SV=3)

HSP 1 Score: 137.117 bits (344), Expect = 2.689e-32
Identity = 66/111 (59.46%), Postives = 86/111 (77.48%), Query Frame = 2

Query:   44 MAFISFSSKTLVLACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            MA I   SK L +A  L   +SL++  DFSIVGYS +DL S ++LI+LF SWM +H K Y+ ++EKL RFE+FKDNLK+IDERNK+++ YWLGLNEF+DLS+ EFK KY+G
Sbjct:    1 MAIICSFSKLLFVAICLFGHMSLSYC-DFSIVGYSQDDLTSTERLIQLFNSWMLKHNKNYKNVDEKLYRFEIFKDNLKYIDERNKMINGYWLGLNEFSDLSNDEFKEKYVG 110

[Close]

BLAST of HO664264 vs. SwissProt
Match: PAPA1_CARPA (Papain OS=Carica papaya PE=1 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 1.334e-31
Identity = 66/111 (59.46%), Postives = 84/111 (75.68%), Query Frame = 2

Query:   44 MAFISFSSKTLVLACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            MA I   SK L +A  L +++ L+F  DFSIVGYS  DL S ++LI+LFESWM +H KIY+ I+EK+ RFE+FKDNLK+IDE NK  ++YWLGLN FAD+S+ EFK KY G
Sbjct:    1 MAMIPSISKLLFVAICLFVYMGLSFG-DFSIVGYSQNDLTSTERLIQLFESWMLKHNKIYKNIDEKIYRFEIFKDNLKYIDETNKKNNSYWLGLNVFADMSNDEFKEKYTG 110

[Close]

BLAST of HO664264 vs. SwissProt
Match: PAPA3_CARPA (Caricain OS=Carica papaya PE=1 SV=2)

HSP 1 Score: 132.494 bits (332), Expect = 6.622e-31
Identity = 65/111 (58.56%), Postives = 82/111 (73.87%), Query Frame = 2

Query:   44 MAFISFSSKTLVLACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            MA I   SK L +A  L + +S++F  DFSIVGYS +DL S ++LI+LF SWM  H K YE ++EKL RFE+FKDNL +IDE NK  ++YWLGLNEFADLS+ EF  KY+G
Sbjct:    1 MAMIPSISKLLFVAICLFVHMSVSFG-DFSIVGYSQDDLTSTERLIQLFNSWMLNHNKFYENVDEKLYRFEIFKDNLNYIDETNKKNNSYWLGLNEFADLSNDEFNEKYVG 110

[Close]

BLAST of HO664264 vs. SwissProt
Match: PAPA2_CARPA (Chymopapain OS=Carica papaya PE=1 SV=2)

HSP 1 Score: 129.028 bits (323), Expect = 7.322e-30
Identity = 63/111 (56.76%), Postives = 85/111 (76.58%), Query Frame = 2

Query:   44 MAFISFSSKTLVLACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            MA +S  SK + LA  L + + L+ A DF  VGYS +DL S+++LI+LF+SWM +H KIYE+I+EK+ RFE+F+DNL +IDE NK  ++YWLGLN FADLS+ EFK KY+G
Sbjct:    1 MATMSSISKIIFLATCLIIHMGLSSA-DFYTVGYSQDDLTSIERLIQLFDSWMLKHNKIYESIDEKIYRFEIFRDNLMYIDETNKKNNSYWLGLNGFADLSNDEFKKKYVG 110

[Close]

BLAST of HO664264 vs. SwissProt
Match: ORYA_ORYSJ (Oryzain alpha chain OS=Oryza sativa subsp. japonica GN=Os04g0650000 PE=1 SV=2)

HSP 1 Score: 77.0258 bits (188), Expect = 3.302e-14
Identity = 42/102 (41.18%), Postives = 63/102 (61.76%), Query Frame = 2

Query:   83 ACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKI----VSNYWLGLNEFADLSHQEFKNKYLG 376
            A +L L +SLA A D SIV Y     +S ++   L+  W + HGK Y  + E+  R+  F+DNL++IDE N      V ++ LGLN FADL+++E+++ YLG
Sbjct:    9 AAALLLLLSLA-AADMSIVSYGE---RSEEEARRLYAEWKAEHGKSYNAVGEEERRYAAFRDNLRYIDEHNAAADAGVHSFRLGLNRFADLTNEEYRDTYLG 106

[Close]

BLAST of HO664264 vs. SwissProt
Match: RD21A_ARATH (Cysteine proteinase RD21a OS=Arabidopsis thaliana GN=RD21A PE=1 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 7.356e-14
Identity = 43/119 (36.13%), Postives = 72/119 (60.50%), Query Frame = 2

Query:   44 MAFISFSSKTLVLACSLCLFISLAFARDFSIVGY------SSEDLKSMDKLIELFESWMSRHGKIYE--TIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            M F+  +   L LA      ++++ A D SI+ Y      S+   +S  +++ ++E+W+ +HGK     ++ EK  RFE+FKDNL+ +DE N+   +Y LGL  FADL++ E+++KYLG
Sbjct:    1 MGFLKPTMAILFLA-----MVAVSSAVDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNSLVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLG 114

[Close]

BLAST of HO664264 vs. SwissProt
Match: ACTN_ACTDE (Actinidain OS=Actinidia deliciosa PE=1 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 6.227e-13
Identity = 42/120 (35.00%), Postives = 74/120 (61.67%), Query Frame = 2

Query:   20 GITKHILAMAFISFSSKTLVLACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVS-NYWLGLNEFADLSHQEFKNKYLG 376
            G+ K  ++M+ + FS+          L +SLAF    ++   +++++K+M      +ESW+ ++GK Y ++ E   RFE+FK+ L+ IDE N   + +Y +GLN+FADL+ +EF++ YLG
Sbjct:    2 GLPKSFVSMSLLFFST---------LLILSLAFNAK-NLTQRTNDEVKAM------YESWLIKYGKSYNSLGEWERRFEIFKETLRFIDEHNADTNRSYKVGLNQFADLTDEEFRSTYLG 105

[Close]

BLAST of HO664264 vs. SwissProt
Match: CPR3_ARATH (Probable cysteine proteinase At4g11310 OS=Arabidopsis thaliana GN=At4g11310 PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 1.062e-12
Identity = 44/113 (38.94%), Postives = 65/113 (57.52%), Query Frame = 2

Query:   62 SSKTLVLACSLCLFI-SLAFARDFSIVGYSSEDLKSMDKLIE-----LFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLGRD 382
            S+K+ +L   + + I S A A D S+V Y  +D   +  + +     +FESWM +HGK+Y ++ EK  R  +F+DNL+ I+ RN    +Y LGL  FADLS  E+K    G D
Sbjct:    3 SAKSAMLILLVAMVIASCATAIDMSVVSY--DDNNRLHSVFDAEASLIFESWMVKHGKVYGSVAEKERRLTIFEDNLRFINNRNAENLSYRLGLTGFADLSLHEYKEVCHGAD 113

[Close]

BLAST of HO664264 vs. TrEMBL
Match: Q6Y1E9_TRIRP (Cysteine protease 14 OS=Trifolium repens PE=2 SV=1)

HSP 1 Score: 213.001 bits (541), Expect = 7.865e-54
Identity = 106/111 (95.50%), Postives = 107/111 (96.40%), Query Frame = 2

Query:   44 MAFISFSSKTLVLACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            MAF  FSSKTLVL CSLCLF+SLAF RDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG
Sbjct:    1 MAF--FSSKTLVLTCSLCLFLSLAFGRDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 109

[Close]

BLAST of HO664264 vs. TrEMBL
Match: Q6Y1F0_TRIRP (Cysteine protease 14 OS=Trifolium repens PE=3 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 1.752e-53
Identity = 105/111 (94.59%), Postives = 107/111 (96.40%), Query Frame = 2

Query:   44 MAFISFSSKTLVLACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            MAF  FSSKTLVL CSLCLF+SLAF RDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHID+RNKIVSNYWLGLNEFADLSHQEFKNKYLG
Sbjct:    1 MAF--FSSKTLVLTCSLCLFLSLAFGRDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDDRNKIVSNYWLGLNEFADLSHQEFKNKYLG 109

[Close]

BLAST of HO664264 vs. TrEMBL
Match: C6T8J0_SOYBN (Putative uncharacterized protein (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 201.06 bits (510), Expect = 3.093e-50
Identity = 100/111 (90.09%), Postives = 105/111 (94.59%), Query Frame = 2

Query:   44 MAFISFSSKTLVLACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            MAF SFS K LVLACS CLF SLAF RDFSIVGYSSEDLKSMDKLIELFESWMS+HGKIY++IEEKLLRFE+FKDNLKHIDERNK+VSNYWLGLNEFADLSHQEFKNKYLG
Sbjct:    1 MAF-SFS-KALVLACSFCLFASLAFGRDFSIVGYSSEDLKSMDKLIELFESWMSKHGKIYQSIEEKLLRFEIFKDNLKHIDERNKVVSNYWLGLNEFADLSHQEFKNKYLG 109

[Close]

BLAST of HO664264 vs. TrEMBL
Match: C6TLR4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 3.781e-48
Identity = 95/106 (89.62%), Postives = 99/106 (93.40%), Query Frame = 2

Query:   62 SSKTLVL-ACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            SSK LVL ACS CLF SLAF RDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYE IEEKLLRFE+FKDNLKHIDERNK+VSNYWLGL+EFADLSH+EF NKYLG
Sbjct:    5 SSKALVLIACSFCLFASLAFGRDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYENIEEKLLRFEIFKDNLKHIDERNKVVSNYWLGLSEFADLSHREFNNKYLG 110

[Close]

BLAST of HO664264 vs. TrEMBL
Match: D7U4Q8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_38.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00023863001 PE=4 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 4.794e-35
Identity = 73/108 (67.59%), Postives = 88/108 (81.48%), Query Frame = 2

Query:   44 MAFISFSSKTLVLACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNK 367
            MA +S  SK  +LA SL L    + ARDFSIVGYS EDL  +DKLI  FESW+S+HGK+Y+++EEKL RFEVF++NL HIDERNK VS+YWLGLNEFADLSH+EFK+K
Sbjct:    1 MALLSSFSKVSILALSLSLLAFSSLARDFSIVGYSPEDLTCIDKLIARFESWVSKHGKVYKSMEEKLHRFEVFRENLNHIDERNKEVSSYWLGLNEFADLSHEEFKSK 108

[Close]

BLAST of HO664264 vs. TrEMBL
Match: B9GPP4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_753865 PE=3 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 6.261e-35
Identity = 70/95 (73.68%), Postives = 81/95 (85.26%), Query Frame = 2

Query:   92 LCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            +  F +   ARDFSIVGY+ EDL S DK+I+LFESW+S+HGKIYE+IEEK LRFE+FKDNL HIDE NK V NYWLGLNEF+DLSH+EFKNKYLG
Sbjct:    1 MSFFANSGLARDFSIVGYTPEDLTSGDKIIDLFESWISKHGKIYESIEEKWLRFEIFKDNLFHIDETNKKVVNYWLGLNEFSDLSHEEFKNKYLG 95

[Close]

BLAST of HO664264 vs. TrEMBL
Match: B9MZ15_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_810417 PE=3 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 5.300e-34
Identity = 70/103 (67.96%), Postives = 83/103 (80.58%), Query Frame = 2

Query:   68 KTLVLACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            KT  L     LF+    A DFSIVGYS E L S+DKL+ELFESW+S HGK Y ++EEKL RFEVFK+NLKHID+RNK V++YWLGLNEFADLSH+EFK+K+LG
Sbjct:    7 KTSFLTFFASLFVCSVLAHDFSIVGYSPEHLTSVDKLVELFESWISGHGKAYNSLEEKLHRFEVFKENLKHIDQRNKEVTSYWLGLNEFADLSHEEFKSKFLG 109

[Close]

BLAST of HO664264 vs. TrEMBL
Match: B9H5V8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_715679 PE=3 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 9.041e-34
Identity = 69/95 (72.63%), Postives = 79/95 (83.16%), Query Frame = 2

Query:   92 LCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            +  F S   ARDFSIVGY+ EDL S D++I+LFESW+S+H KIYE+IEEK  RFE+FKDNL HIDE NK V NYWLGLNEFADLSH+EFKNKYLG
Sbjct:    1 MSFFASSCLARDFSIVGYAPEDLTSRDRIIDLFESWISKHQKIYESIEEKWHRFEIFKDNLFHIDETNKKVVNYWLGLNEFADLSHEEFKNKYLG 95

[Close]

BLAST of HO664264 vs. TrEMBL
Match: Q84M26_HELAN (Cysteine protease-4 OS=Helianthus annuus GN=scp4 PE=2 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 1.181e-33
Identity = 68/111 (61.26%), Postives = 87/111 (78.38%), Query Frame = 2

Query:   44 MAFISFSSKTLVLACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            MAFI  S KT +    + +    A A +FSI+GY+ EDL S+ K+I LFESW+++H KIYE+++EKL RFE+F DNLKHID+ NK VSNYWLGLNEFADL+H+EFKNK+LG
Sbjct:    1 MAFIFSSKKTSLFLVFVSVLACSALANEFSILGYAPEDLTSIHKVIHLFESWLAKHSKIYESLDEKLHRFEIFMDNLKHIDDTNKKVSNYWLGLNEFADLTHEEFKNKFLG 111

[Close]

BLAST of HO664264 vs. TrEMBL
Match: D7KJ66_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_472321 PE=4 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 3.436e-33
Identity = 69/93 (74.19%), Postives = 79/93 (84.95%), Query Frame = 2

Query:   98 LFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            L +S+A + D+SIVGYS EDL+S DKLIELFE+W+S   K YET+EEKLLRFEVFKDNLKHIDE NK V +YWLGLNEFADLSH+EFK  YLG
Sbjct:   21 LSLSVAASHDYSIVGYSPEDLESHDKLIELFENWISNFEKAYETVEEKLLRFEVFKDNLKHIDETNKKVKSYWLGLNEFADLSHEEFKKMYLG 113

[Close]

BLAST of HO664264 vs. TAIR peptide
Match: AT4G35350.2 (| Symbols: XCP1 | xylem cysteine peptidase 1 | chr4:16810529-16811578 FORWARD LENGTH=288)

HSP 1 Score: 143.28 bits (360), Expect = 3.732e-35
Identity = 66/113 (58.41%), Postives = 88/113 (77.88%), Query Frame = 2

Query:   38 LAMAFISFSSKTLVLACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            +A +  S S  +L++A S    +  AFARDFSIVGY+ E L + DKL+ELFESWMS H K Y+++EEK+ RFEVF++NL HID+RN  +++YWLGLNEFADL+H+EFK +YLG
Sbjct:    1 MAFSAPSLSKFSLLVAISASALLCCAFARDFSIVGYTPEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEEFKGRYLG 113

[Close]

BLAST of HO664264 vs. TAIR peptide
Match: AT4G35350.1 (| Symbols: XCP1 | xylem cysteine peptidase 1 | chr4:16810529-16811875 FORWARD LENGTH=355)

HSP 1 Score: 143.28 bits (360), Expect = 3.732e-35
Identity = 66/113 (58.41%), Postives = 88/113 (77.88%), Query Frame = 2

Query:   38 LAMAFISFSSKTLVLACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            +A +  S S  +L++A S    +  AFARDFSIVGY+ E L + DKL+ELFESWMS H K Y+++EEK+ RFEVF++NL HID+RN  +++YWLGLNEFADL+H+EFK +YLG
Sbjct:    1 MAFSAPSLSKFSLLVAISASALLCCAFARDFSIVGYTPEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEEFKGRYLG 113

[Close]

BLAST of HO664264 vs. TAIR peptide
Match: AT1G20850.1 (| Symbols: XCP2 | xylem cysteine peptidase 2 | chr1:7252208-7253537 FORWARD LENGTH=356)

HSP 1 Score: 141.354 bits (355), Expect = 1.418e-34
Identity = 72/111 (64.86%), Postives = 84/111 (75.68%), Query Frame = 2

Query:   53 ISFSSKTLVLACSLC---LFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            +S  S+ L  A +L    L +S A + D+SIVGYS EDL+S DKLIELFE+W+S   K YET+EEK LRFEVFKDNLKHIDE NK   +YWLGLNEFADLSH+EFK  YLG
Sbjct:    3 LSSPSRILCFALALSAASLSLSFASSHDYSIVGYSPEDLESHDKLIELFENWISNFEKAYETVEEKFLRFEVFKDNLKHIDETNKKGKSYWLGLNEFADLSHEEFKKMYLG 113

[Close]

BLAST of HO664264 vs. TAIR peptide
Match: AT5G43060.1 (| Symbols: | Granulin repeat cysteine protease family protein | chr5:17269784-17272117 REVERSE LENGTH=463)

HSP 1 Score: 77.0258 bits (188), Expect = 3.285e-15
Identity = 46/121 (38.02%), Postives = 69/121 (57.02%), Query Frame = 2

Query:   44 MAFISFSSKTLVLACSLCLFISLAFARDFSIVGY------SSEDLKSMDKLIELFESWMSRHGKIYETIE----EKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            M F+  S   L+LA      I +++A D SI+ Y      ++E  +S  ++  ++E+WM  HGK          EK  RFE+FKDNL+ IDE N    +Y LGL  FADL+++E+++ YLG
Sbjct:    1 MGFLKLSPMILLLA-----MIGVSYAMDMSIISYDENHHITTETSRSDSEVERIYEAWMVEHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLG 116

[Close]

BLAST of HO664264 vs. TAIR peptide
Match: AT1G47128.1 (| Symbols: RD21, RD21A | Granulin repeat cysteine protease family protein | chr1:17283139-17285609 REVERSE LENGTH=462)

HSP 1 Score: 75.8702 bits (185), Expect = 7.317e-15
Identity = 43/119 (36.13%), Postives = 72/119 (60.50%), Query Frame = 2

Query:   44 MAFISFSSKTLVLACSLCLFISLAFARDFSIVGY------SSEDLKSMDKLIELFESWMSRHGKIYE--TIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            M F+  +   L LA      ++++ A D SI+ Y      S+   +S  +++ ++E+W+ +HGK     ++ EK  RFE+FKDNL+ +DE N+   +Y LGL  FADL++ E+++KYLG
Sbjct:    1 MGFLKPTMAILFLA-----MVAVSSAVDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNSLVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLG 114

[Close]

BLAST of HO664264 vs. TAIR peptide
Match: AT5G50260.1 (| Symbols: | Cysteine proteinases superfamily protein | chr5:20455605-20456862 FORWARD LENGTH=361)

HSP 1 Score: 72.4034 bits (176), Expect = 8.090e-14
Identity = 37/99 (37.37%), Postives = 59/99 (59.60%), Query Frame = 2

Query:   80 LACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLG 376
            +  +LC+ + L   +      + ++D++S + L EL+E W S H  +  ++EEK  RF VFK N+KHI E NK   +Y L LN+F D++ +EF+  Y G
Sbjct:    5 IVLALCMLMVLETTKGLD---FHNKDVESENSLWELYERWRSHH-TVARSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAG 99

[Close]

BLAST of HO664264 vs. TAIR peptide
Match: AT4G11310.1 (| Symbols: | Papain family cysteine protease | chr4:6883594-6885318 FORWARD LENGTH=364)

HSP 1 Score: 72.0182 bits (175), Expect = 1.057e-13
Identity = 44/113 (38.94%), Postives = 65/113 (57.52%), Query Frame = 2

Query:   62 SSKTLVLACSLCLFI-SLAFARDFSIVGYSSEDLKSMDKLIE-----LFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLGRD 382
            S+K+ +L   + + I S A A D S+V Y  +D   +  + +     +FESWM +HGK+Y ++ EK  R  +F+DNL+ I+ RN    +Y LGL  FADLS  E+K    G D
Sbjct:    3 SAKSAMLILLVAMVIASCATAIDMSVVSY--DDNNRLHSVFDAEASLIFESWMVKHGKVYGSVAEKERRLTIFEDNLRFINNRNAENLSYRLGLTGFADLSLHEYKEVCHGAD 113

[Close]

BLAST of HO664264 vs. TAIR peptide
Match: AT1G09850.1 (| Symbols: XBCP3 | xylem bark cysteine peptidase 3 | chr1:3201848-3203875 FORWARD LENGTH=437)

HSP 1 Score: 70.4774 bits (171), Expect = 3.074e-13
Identity = 42/100 (42.00%), Postives = 57/100 (57.00%), Query Frame = 2

Query:   80 LACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIV-SNYWLGLNEFADLSHQEFKNKYLG 376
            ++ S   FISL F   F ++  SS    S D + ELF+ W  +HGK Y + EE+  R ++FKDN   + + N I  + Y L LN FADL+H EFK   LG
Sbjct:    1 MSMSSSSFISLTF---FFLLLVSSSS--SSDDISELFDDWCQKHGKTYGSEEERQQRIQIFKDNHDFVTQHNLITNATYSLSLNAFADLTHHEFKASRLG 95

[Close]

BLAST of HO664264 vs. TAIR peptide
Match: AT3G43960.1 (| Symbols: | Cysteine proteinases superfamily protein | chr3:15774122-15775628 REVERSE LENGTH=376)

HSP 1 Score: 69.707 bits (169), Expect = 5.244e-13
Identity = 39/109 (35.78%), Postives = 65/109 (59.63%), Query Frame = 2

Query:   53 ISFSSKTLVLACSLCLFISLAFARDFSIVGYSSEDLKSMDKLIELFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVS-NYWLGLNEFADLSHQEFKNKYLG 376
            ++ S +TL L     L IS++       V  ++E  ++  +++ ++E W+  +GK Y  + EK  RF++FKDNLK I+E N   + +Y  GLN+F+DL+  EF+  YLG
Sbjct:    1 MAISFRTLALLTLSVLLISISLG-----VVTATESQRNEGEVLTMYEQWLVENGKNYNGLGEKERRFKIFKDNLKRIEEHNSDPNRSYERGLNKFSDLTADEFQASYLG 104

[Close]

BLAST of HO664264 vs. TAIR peptide
Match: AT4G11320.1 (| Symbols: | Papain family cysteine protease | chr4:6887336-6888827 FORWARD LENGTH=371)

HSP 1 Score: 67.3958 bits (163), Expect = 2.602e-12
Identity = 32/66 (48.48%), Postives = 43/66 (65.15%), Query Frame = 2

Query:  185 LFESWMSRHGKIYETIEEKLLRFEVFKDNLKHIDERNKIVSNYWLGLNEFADLSHQEFKNKYLGRD 382
            +FESWM +HGK+Y+++ EK  R  +F+DNL+ I  RN    +Y LGLN FADLS  E+     G D
Sbjct:   55 MFESWMVKHGKVYDSVAEKERRLTIFEDNLRFITNRNAENLSYRLGLNRFADLSLHEYGEICHGAD 120

The following BLAST results are available for this feature:

BLAST of HO664264 vs. SwissProt
BLAST of HO664264 vs. TrEMBL
BLAST of HO664264 vs. TAIR peptide

BLAST of HO664264 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
XCP1_ARATH	3.752e-34	58.41	Xylem cysteine proteinase 1 OS=Arabidopsis thalian...	[more]
XCP2_ARATH	1.426e-33	64.86	Xylem cysteine proteinase 2 OS=Arabidopsis thalian...	[more]
PAPA4_CARPA	2.689e-32	59.46	Papaya proteinase 4 OS=Carica papaya PE=1 SV=3	[more]
PAPA1_CARPA	1.334e-31	59.46	Papain OS=Carica papaya PE=1 SV=1	[more]
PAPA3_CARPA	6.622e-31	58.56	Caricain OS=Carica papaya PE=1 SV=2	[more]
PAPA2_CARPA	7.322e-30	56.76	Chymopapain OS=Carica papaya PE=1 SV=2	[more]
ORYA_ORYSJ	3.302e-14	41.18	Oryzain alpha chain OS=Oryza sativa subsp. japonic...	[more]
RD21A_ARATH	7.356e-14	36.13	Cysteine proteinase RD21a OS=Arabidopsis thaliana ...	[more]
ACTN_ACTDE	6.227e-13	35.00	Actinidain OS=Actinidia deliciosa PE=1 SV=1	[more]
CPR3_ARATH	1.062e-12	38.94	Probable cysteine proteinase At4g11310 OS=Arabidop...	[more]

BLAST of HO664264 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q6Y1E9_TRIRP	7.865e-54	95.50	Cysteine protease 14 OS=Trifolium repens PE=2 SV=1	[more]
Q6Y1F0_TRIRP	1.752e-53	94.59	Cysteine protease 14 OS=Trifolium repens PE=3 SV=1	[more]
C6T8J0_SOYBN	3.093e-50	90.09	Putative uncharacterized protein (Fragment) OS=Gly...	[more]
C6TLR4_SOYBN	3.781e-48	89.62	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7U4Q8_VITVI	4.794e-35	67.59	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9GPP4_POPTR	6.261e-35	73.68	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9MZ15_POPTR	5.300e-34	67.96	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9H5V8_POPTR	9.041e-34	72.63	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q84M26_HELAN	1.181e-33	61.26	Cysteine protease-4 OS=Helianthus annuus GN=scp4 P...	[more]
D7KJ66_ARALY	3.436e-33	74.19	Putative uncharacterized protein OS=Arabidopsis ly...	[more]

BLAST of HO664264 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT4G35350.2	3.732e-35	58.41	\| Symbols: XCP1 \| xylem cysteine peptidase 1 \| chr...	[more]
AT4G35350.1	3.732e-35	58.41	\| Symbols: XCP1 \| xylem cysteine peptidase 1 \| chr...	[more]
AT1G20850.1	1.418e-34	64.86	\| Symbols: XCP2 \| xylem cysteine peptidase 2 \| chr...	[more]
AT5G43060.1	3.285e-15	38.02	\| Symbols: \| Granulin repeat cysteine protease fa...	[more]
AT1G47128.1	7.317e-15	36.13	\| Symbols: RD21, RD21A \| Granulin repeat cysteine ...	[more]
AT5G50260.1	8.090e-14	37.37	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT4G11310.1	1.057e-13	38.94	\| Symbols: \| Papain family cysteine protease \| ch...	[more]
AT1G09850.1	3.074e-13	42.00	\| Symbols: XBCP3 \| xylem bark cysteine peptidase 3...	[more]
AT3G43960.1	5.244e-13	35.78	\| Symbols: \| Cysteine proteinases superfamily pro...	[more]
AT4G11320.1	2.602e-12	48.48	\| Symbols: \| Papain family cysteine protease \| ch...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013128	Peptidase C1A, papain	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 43..125 score: 6.5
IPR013201	Proteinase inhibitor I29, cathepsin propeptide	PFAM	PF08246	Inhibitor_I29	coord: 63..119 score: 7.3
IPR013201	Proteinase inhibitor I29, cathepsin propeptide	SMART	SM00848	no description	coord: 63..119 score: 8.6
None	No IPR available	GENE3D	3.90.70.10	no description	coord: 44..126 score: 4.1
None	No IPR available	PANTHER	PTHR12411:SF44	CYSTEINE PROTEASE	coord: 43..125 score: 6.5
None	No IPR available	SUPERFAMILY	54001	Cysteine proteinases	coord: 53..126 score: 4.5
None	No IPR available	SEG	seg	seg	coord: 9..24 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO664264 ID=HO664264; Name=HO664264; organism=Cicer arietinum; type=EST; length=385bp
TCGAGCGGCCGCCCGGGCAGGTATTACCAAACACATTCTTGCAATGGCTT
TTATATCTTTTTCCTCCAAAACACTTGTTCTGGCTTGTTCCTTATGCTTA
TTTATTTCTTTAGCATTTGCACGTGATTTCTCCATCGTGGGGTATTCATC
TGAGGACTTAAAGTCCATGGACAAGCTCATTGAACTCTTCGAATCATGGA
TGTCGAGACACGGCAAGATTTATGAGACAATTGAGGAAAAACTACTTAGG
TTCGAGGTGTTTAAGGATAATCTAAAGCACATTGATGAGAGAAACAAGAT
TGTTAGCAACTATTGGCTTGGTTTGAATGAGTTTGCTGATTTGAGTCACC
AAGAGTTCAAGAACAAGTACCTCGGCCGCGACCAC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008234	cysteine-type peptidase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR013128	Peptidase_C1A
IPR013201	Prot_inhib_I29

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