FD449481

EST Overview


Name	FD449481
Unique Name	FD449481
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	360

Libraries


Library Name	Type
LIBEST_022499 Chickpea Ascochyta-Induced Library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FD449481 vs. SwissProt
Match: C82A1_PEA (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 114.005 bits (284), Expect = 2.439e-25
Identity = 57/69 (82.61%), Postives = 61/69 (88.41%), Query Frame = -2

Query:  153 SGRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCYETM 359
            SGRRMC GM LGL+M+HYILANFLHSFEILN S ESIDVTEVLEF +TKAT LEVLVKP LS KCYE+M
Sbjct:  476 SGRRMCAGMSLGLHMVHYILANFLHSFEILNPSPESIDVTEVLEFVTTKATPLEVLVKPCLSFKCYESM 544

[Close]

BLAST of FD449481 vs. SwissProt
Match: C82A3_SOYBN (Cytochrome P450 82A3 OS=Glycine max GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 2.887e-18
Identity = 44/69 (63.77%), Postives = 53/69 (76.81%), Query Frame = -2

Query:  153 SGRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCYETM 359
            SGRR+C GM LGLNM+H+ LAN LHSF+ILN S E +D+TE   FT+TKAT LE+LVKP  S   YET+
Sbjct:  459 SGRRVCAGMSLGLNMVHFTLANLLHSFDILNPSAEPVDMTEFFGFTNTKATPLEILVKPRQSPNYYETL 527

[Close]

BLAST of FD449481 vs. SwissProt
Match: C82A4_SOYBN (Cytochrome P450 82A4 OS=Glycine max GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.872e-17
Identity = 43/69 (62.32%), Postives = 52/69 (75.36%), Query Frame = -2

Query:  153 SGRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCYETM 359
            SGRR+CPG+  GL  +H  LA+FLHSFEILN S E +D+TE    T+TKAT LEVLVKP LS  CY++M
Sbjct:  457 SGRRVCPGISFGLQTVHLALASFLHSFEILNPSTEPLDMTEAFGVTNTKATPLEVLVKPCLSPSCYKSM 525

[Close]

BLAST of FD449481 vs. SwissProt
Match: C82A2_SOYBN (Cytochrome P450 82A2 OS=Glycine max GN=CYP82A2 PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 7.112e-17
Identity = 41/68 (60.29%), Postives = 52/68 (76.47%), Query Frame = -2

Query:  153 GRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCYETM 356
            GRR+CPG+ LGL  +   LA+FLHSFEILN S E +D+TEV   T+TKAT LE+L+KP LS  CYE++
Sbjct:  455 GRRICPGINLGLQTVRLTLASFLHSFEILNPSTEPLDMTEVFRATNTKATPLEILIKPRLSPSCYESI 522

[Close]

BLAST of FD449481 vs. TrEMBL
Match: Q9FVK6_PEA (Wound-inducible P450 hydroxylase OS=Pisum sativum GN=CYP82A1 PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 4.999e-24
Identity = 57/69 (82.61%), Postives = 61/69 (88.41%), Query Frame = -2

Query:  153 SGRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCYETM 359
            SGRRMC GM LGL+M+HYILANFLHSFEILN S ESIDVTEVLEF +TKAT LEVLVKP LS KCYE+M
Sbjct:  472 SGRRMCAGMSLGLHMVHYILANFLHSFEILNPSPESIDVTEVLEFVTTKATPLEVLVKPCLSFKCYESM 540

[Close]

BLAST of FD449481 vs. TrEMBL
Match: B9S8Q1_RICCO (Cytochrome P450, putative OS=Ricinus communis GN=RCOM_0603590 PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 4.390e-12
Identity = 35/67 (52.24%), Postives = 48/67 (71.64%), Query Frame = -2

Query:  159 SGRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCYE 359
            SGRR CPGM LGL ++H+ILA+FLH FE+   S E++D+TE    T+ KAT+LEV + P L+   Y+
Sbjct:  454 SGRRSCPGMALGLQVVHFILASFLHGFEVAKASGENVDMTESTGLTNLKATSLEVHLVPRLNSTLYK 520

[Close]

BLAST of FD449481 vs. TrEMBL
Match: D7LK52_ARALY (CYP82F1 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_901127 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 8.278e-11
Identity = 31/66 (46.97%), Postives = 44/66 (66.67%), Query Frame = -2

Query:  159 GRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCYE 356
            GRR CP +PLG+ M+HY+LA FLHSF++   S + +D+TE     + KAT+LEV + P L    Y+
Sbjct:  454 GRRSCPAIPLGMKMVHYLLARFLHSFDLAKPSSQDVDMTESNGLVNHKATSLEVFITPRLHKSLYK 519

[Close]

BLAST of FD449481 vs. TrEMBL
Match: D7UE12_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_255.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00005088001 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.146e-10
Identity = 32/66 (48.48%), Postives = 44/66 (66.67%), Query Frame = -2

Query:  162 SGRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCY 359
            SGRR CPG+ L L ++H+ LA+ LHS+E+   S   +D+TE L  T+ KAT LEVL+ P L  + Y
Sbjct:  476 SGRRSCPGISLALQVVHFALASLLHSYEVTKPSDGDVDMTESLGLTNLKATPLEVLLSPRLKAELY 541

[Close]

BLAST of FD449481 vs. TrEMBL
Match: A5AKY1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_002367 PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 3.146e-10
Identity = 32/66 (48.48%), Postives = 44/66 (66.67%), Query Frame = -2

Query:  162 SGRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCY 359
            SGRR CPG+ L L ++H+ LA+ LHS+E+   S   +D+TE L  T+ KAT LEVL+ P L  + Y
Sbjct:  461 SGRRSCPGISLALQVVHFALASLLHSYEVTKPSDGDVDMTESLGLTNLKATPLEVLLSPRLKAELY 526

[Close]

BLAST of FD449481 vs. TrEMBL
Match: O48925_SOYBN (CYP82C1p OS=Glycine max GN=CYP82C1 PE=2 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 4.109e-10
Identity = 31/67 (46.27%), Postives = 44/67 (65.67%), Query Frame = -2

Query:  159 SGRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCYE 359
            SGRR CPG  L L ++H  +A  LHSF + + S++ +D+TE +  T+ KAT LE+L+ P L  K YE
Sbjct:  465 SGRRACPGASLALRVVHLTMARLLHSFNVASPSNQVVDMTESIGLTNLKATPLEILLTPRLDTKLYE 531

[Close]

BLAST of FD449481 vs. TrEMBL
Match: Q7Y222_ARATH (At2g25160 OS=Arabidopsis thaliana GN=At2g25160 PE=2 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 5.366e-10
Identity = 31/66 (46.97%), Postives = 42/66 (63.64%), Query Frame = -2

Query:  159 GRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCYE 356
            GRR CP +PLG+ M+HY+L  FLHSF++   S + +D+TE     + KAT LEV + P L    YE
Sbjct:  454 GRRSCPAIPLGMRMVHYLLVRFLHSFDLARPSSQDVDMTESNGLVNHKATPLEVNIIPRLHKSLYE 519

[Close]

BLAST of FD449481 vs. TrEMBL
Match: C6TDR9_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.039e-9
Identity = 32/67 (47.76%), Postives = 42/67 (62.69%), Query Frame = -2

Query:  159 SGRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCYE 359
            SGRR CPG  L L ++H  LA  LHSF + + S++ +D+TE    T+ KAT LEVL+ P    K YE
Sbjct:  104 SGRRACPGASLALRVVHLTLARLLHSFNVASPSNQVVDMTESFGLTNLKATPLEVLLTPRQDTKFYE 170

[Close]

BLAST of FD449481 vs. TrEMBL
Match: B9R7K4_RICCO (Cytochrome P450, putative OS=Ricinus communis GN=RCOM_1592650 PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.663e-9
Identity = 33/66 (50.00%), Postives = 41/66 (62.12%), Query Frame = -2

Query:  162 SGRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCY 359
            SGRR CPG P GL+ LH  LA FLH+F++     + ID++E+      KAT LEVLV P L  K Y
Sbjct:  456 SGRRSCPGAPFGLHALHLALARFLHAFDLATPMDQPIDMSEMPGTHVPKATPLEVLVSPRLPAKLY 521

[Close]

BLAST of FD449481 vs. TrEMBL
Match: O49396_ARATH (Cytochrome P450-like protein OS=Arabidopsis thaliana GN=F10N7.240 PE=1 SV=3)

HSP 1 Score: 64.3142 bits (155), Expect = 4.543e-9
Identity = 32/66 (48.48%), Postives = 41/66 (62.12%), Query Frame = -2

Query:  162 SGRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCY 359
            SGRR CPG  L + MLH  LA FLHSFE+  +    +D++E    T TKAT LEVL+ P L  + +
Sbjct:  446 SGRRSCPGPSLAMQMLHLGLARFLHSFEVKTVLDRPVDMSESPGLTITKATPLEVLINPRLKRELF 511

[Close]

BLAST of FD449481 vs. TAIR peptide
Match: AT2G25160.1 (| Symbols: CYP82F1 | cytochrome P450, family 82, subfamily F, polypeptide 1 | chr2:10709236-10711211 REVERSE LENGTH=527)

HSP 1 Score: 67.3958 bits (163), Expect = 2.088e-12
Identity = 31/66 (46.97%), Postives = 42/66 (63.64%), Query Frame = -2

Query:  159 GRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCYE 356
            GRR CP +PLG+ M+HY+L  FLHSF++   S + +D+TE     + KAT LEV + P L    YE
Sbjct:  454 GRRSCPAIPLGMRMVHYLLVRFLHSFDLARPSSQDVDMTESNGLVNHKATPLEVNIIPRLHKSLYE 519

[Close]

BLAST of FD449481 vs. TAIR peptide
Match: AT4G31950.1 (| Symbols: CYP82C3 | cytochrome P450, family 82, subfamily C, polypeptide 3 | chr4:15455163-15457090 FORWARD LENGTH=512)

HSP 1 Score: 64.3142 bits (155), Expect = 1.768e-11
Identity = 32/66 (48.48%), Postives = 41/66 (62.12%), Query Frame = -2

Query:  162 SGRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCY 359
            SGRR CPG  L + MLH  LA FLHSFE+  +    +D++E    T TKAT LEVL+ P L  + +
Sbjct:  446 SGRRSCPGPSLAMQMLHLGLARFLHSFEVKTVLDRPVDMSESPGLTITKATPLEVLINPRLKRELF 511

[Close]

BLAST of FD449481 vs. TAIR peptide
Match: AT4G31940.1 (| Symbols: CYP82C4 | cytochrome P450, family 82, subfamily C, polypeptide 4 | chr4:15452040-15453966 FORWARD LENGTH=524)

HSP 1 Score: 57.7658 bits (138), Expect = 1.655e-9
Identity = 28/59 (47.46%), Postives = 37/59 (62.71%), Query Frame = -2

Query:  183 SGRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKP 359
            SGRR CPG  L + +LH  LA FLHSF++  +    +D++E    T  KAT LEVL+ P
Sbjct:  458 SGRRSCPGSSLAMQVLHLGLARFLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISP 516

[Close]

BLAST of FD449481 vs. TAIR peptide
Match: AT3G25180.1 (| Symbols: CYP82G1 | cytochrome P450, family 82, subfamily G, polypeptide 1 | chr3:9167443-9169270 REVERSE LENGTH=515)

HSP 1 Score: 57.7658 bits (138), Expect = 1.655e-9
Identity = 28/69 (40.58%), Postives = 40/69 (57.97%), Query Frame = -2

Query:  153 SGRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCYETM 359
            SGRR CPG+ LGL ++H++LA  L  FE+  +S E +D+ E       K   +EV+V P L  K Y  +
Sbjct:  447 SGRRSCPGVNLGLRVVHFVLARLLQGFELHKVSDEPLDMAEGPGLALPKINPVEVVVMPRLDPKLYSLL 515

[Close]

BLAST of FD449481 vs. TAIR peptide
Match: AT4G31970.1 (| Symbols: CYP82C2 | cytochrome P450, family 82, subfamily C, polypeptide 2 | chr4:15462408-15464358 FORWARD LENGTH=523)

HSP 1 Score: 57.3806 bits (137), Expect = 2.161e-9
Identity = 29/66 (43.94%), Postives = 38/66 (57.58%), Query Frame = -2

Query:  162 SGRRMCPGMPLGLNMLHYILANFLHSFEILNLSHESIDVTEVLEFTSTKATTLEVLVKPGLSLKCY 359
            SGRR CPG  L + +LH  LA FL SF++  +    +D+TE    T  KAT LE+L+ P L    Y
Sbjct:  457 SGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVMDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522

The following BLAST results are available for this feature:

BLAST of FD449481 vs. SwissProt
BLAST of FD449481 vs. TrEMBL
BLAST of FD449481 vs. TAIR peptide

BLAST of FD449481 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
C82A1_PEA	2.439e-25	82.61	Cytochrome P450 82A1 (Fragment) OS=Pisum sativum G...	[more]
C82A3_SOYBN	2.887e-18	63.77	Cytochrome P450 82A3 OS=Glycine max GN=CYP82A3 PE=...	[more]
C82A4_SOYBN	1.872e-17	62.32	Cytochrome P450 82A4 OS=Glycine max GN=CYP82A4 PE=...	[more]
C82A2_SOYBN	7.112e-17	60.29	Cytochrome P450 82A2 OS=Glycine max GN=CYP82A2 PE=...	[more]

BLAST of FD449481 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9FVK6_PEA	4.999e-24	82.61	Wound-inducible P450 hydroxylase OS=Pisum sativum ...	[more]
B9S8Q1_RICCO	4.390e-12	52.24	Cytochrome P450, putative OS=Ricinus communis GN=R...	[more]
D7LK52_ARALY	8.278e-11	46.97	CYP82F1 OS=Arabidopsis lyrata subsp. lyrata GN=ARA...	[more]
D7UE12_VITVI	3.146e-10	48.48	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5AKY1_VITVI	3.146e-10	48.48	Putative uncharacterized protein OS=Vitis vinifera...	[more]
O48925_SOYBN	4.109e-10	46.27	CYP82C1p OS=Glycine max GN=CYP82C1 PE=2 SV=1	[more]
Q7Y222_ARATH	5.366e-10	46.97	At2g25160 OS=Arabidopsis thaliana GN=At2g25160 PE=...	[more]
C6TDR9_SOYBN	2.039e-9	47.76	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9R7K4_RICCO	2.663e-9	50.00	Cytochrome P450, putative OS=Ricinus communis GN=R...	[more]
O49396_ARATH	4.543e-9	48.48	Cytochrome P450-like protein OS=Arabidopsis thalia...	[more]

BLAST of FD449481 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT2G25160.1	2.088e-12	46.97	\| Symbols: CYP82F1 \| cytochrome P450, family 82, s...	[more]
AT4G31950.1	1.768e-11	48.48	\| Symbols: CYP82C3 \| cytochrome P450, family 82, s...	[more]
AT4G31940.1	1.655e-9	47.46	\| Symbols: CYP82C4 \| cytochrome P450, family 82, s...	[more]
AT3G25180.1	1.655e-9	40.58	\| Symbols: CYP82G1 \| cytochrome P450, family 82, s...	[more]
AT4G31970.1	2.161e-9	43.94	\| Symbols: CYP82C2 \| cytochrome P450, family 82, s...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	1.10.630.10	no description	coord: 1..59 score: 1.1
IPR001128	Cytochrome P450	PANTHER	PTHR19383	CYTOCHROME P450	coord: 1..27 score: 6.1
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..36 score: 3.7
IPR001128	Cytochrome P450	SUPERFAMILY	48264	Cytochrome P450	coord: 2..59 score: 2
None	No IPR available	PANTHER	PTHR19383:SF39	CYTOCHROME P450, SUBFAMILY 82	coord: 1..27 score: 6.1

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FD449481 ID=FD449481; Name=FD449481; organism=Cicer arietinum; type=EST; length=360bp
ACAAGAACAACTTATAAATAATTATATATGGTGCAACAAGACAAAACTAA
TTCCAAGATTATTGAGAAACTAACTACAATTAGTTACCACTAATTAAGTA
GTGGTAACATCACATATAAAAACAATAACAATGCAAAGTATTAATAGAAT
CACATGGTCTCATAACATTTAAGAGACAAACCAGGTTTAACCAAAACTTC
AAGTGTAGTGGCTTTGGTGCTAGTAAACTCAAGCACTTCAGTTACATCAA
TTGATTCATGAGATAAATTCAAGATTTCAAAGGAATGCAAAAAATTAGCT
AGAATATAATGAAGCATGTTCAAACCAAGTGGCATTCCGGGACACATTCT
TCTACCACTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004497	monooxygenase activity
GO:0005506	iron ion binding
GO:0009055	electron transfer activity
GO:0020037	heme binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450

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