HO064832

EST Overview


Name	HO064832
Unique Name	HO064832
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	292

Libraries


Library Name	Type
LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO064832 vs. SwissProt
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 140.969 bits (354), Expect = 1.881e-33
Identity = 70/96 (72.92%), Postives = 75/96 (78.12%), Query Frame = -1

Query:    2 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 289
            YYTDQII GL               LEWSMSNLLNHPEVLKKV+DELDTHVGQDRLVDESDLP LTY++NVI ETLRLY PAPLL+PHS SD+C I
Sbjct:  286 YYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNI 381

[Close]

BLAST of HO064832 vs. SwissProt
Match: C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 2.085e-24
Identity = 50/96 (52.08%), Postives = 67/96 (69.79%), Query Frame = -1

Query:    2 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 289
            YYTDQII G++              LEW++SNLLNHP+V+ K RDE+D  VG DRL++E+DL  L Y++N++ ETLRL+   PLL+PH AS+DC I
Sbjct:  290 YYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKI 385

[Close]

BLAST of HO064832 vs. SwissProt
Match: C81F1_ARATH (Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 97.8265 bits (242), Expect = 1.826e-20
Identity = 48/93 (51.61%), Postives = 62/93 (66.67%), Query Frame = -1

Query:   11 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDD 289
            YYTD II GL+              LEW+M+NLL +PEVL+K R E+D  +G+DRL+DESD+  L Y+QNV+ ET RL+  AP LIP S +DD
Sbjct:  284 YYTDVIIKGLMMSMMLAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDESDIAVLPYLQNVVSETFRLFPVAPFLIPRSPTDD 376

[Close]

BLAST of HO064832 vs. SwissProt
Match: C82A2_SOYBN (Cytochrome P450 82A2 OS=Glycine max GN=CYP82A2 PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.147e-14
Identity = 37/69 (53.62%), Postives = 49/69 (71.01%), Query Frame = -1

Query:    2 WSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 208
            W+ S +LN+P VL+K++ ELD  VG++R + ESDL  LTY+Q V+ ETLRLY PAPL  P    +DC I
Sbjct:  329 WATSLILNNPSVLEKLKAELDIQVGKERYICESDLSKLTYLQAVVKETLRLYPPAPLSRPREFEEDCTI 397

[Close]

BLAST of HO064832 vs. SwissProt
Match: C82A1_PEA (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 78.5666 bits (192), Expect = 1.147e-14
Identity = 34/69 (49.28%), Postives = 50/69 (72.46%), Query Frame = -1

Query:    2 WSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 208
            W+M  LL HP VL+K+++EL+T++G++R V+ESD+  L Y+  +I ETLRLY PAP   P   ++DC I
Sbjct:  351 WAMCLLLKHPHVLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTI 419

[Close]

BLAST of HO064832 vs. SwissProt
Match: F3PH_ARATH (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 3.336e-14
Identity = 37/94 (39.36%), Postives = 56/94 (59.57%), Query Frame = -1

Query:    2 TDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 283
            TD  I  L+              ++W+++ L+ HP+++ K ++ELD  VG+DR V+ESD+  L Y+Q VI E  RL+ P PL +PH AS+ C I
Sbjct:  288 TDTEIKALLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKENFRLHPPTPLSLPHIASESCEI 381

[Close]

BLAST of HO064832 vs. SwissProt
Match: C98A1_SORBI (Cytochrome P450 98A1 OS=Sorghum bicolor GN=CYP98A1 PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 9.706e-14
Identity = 33/65 (50.77%), Postives = 50/65 (76.92%), Query Frame = -1

Query:   17 EWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSAS 211
            EW+M+ L+ +P V KK+++ELD  VG+DR++ E+D  NL Y+Q V+ E+LRL+ P PL++PH AS
Sbjct:  312 EWAMAELVRNPRVQKKLQEELDRVVGRDRVMLETDFQNLPYLQAVVKESLRLHPPTPLMLPHKAS 376

[Close]

BLAST of HO064832 vs. SwissProt
Match: C93A1_SOYBN (Cytochrome P450 93A1 OS=Glycine max GN=CYP93A1 PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.817e-13
Identity = 31/64 (48.44%), Postives = 49/64 (76.56%), Query Frame = -1

Query:   20 EWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSA 211
            EW+M+ L+N+P+VL+K R E+D  VG+ R+V+ESD+ NL Y+Q ++ ETLRL+   PL++  S+
Sbjct:  316 EWAMAELINNPDVLEKARQEIDAVVGKSRMVEESDIANLPYLQAIVRETLRLHPGGPLVVRESS 379

[Close]

BLAST of HO064832 vs. SwissProt
Match: C79A2_ARATH (Phenylalanine N-hydroxylase OS=Arabidopsis thaliana GN=CYP79A2 PE=1 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.817e-13
Identity = 30/70 (42.86%), Postives = 48/70 (68.57%), Query Frame = -1

Query:    2 EWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 211
            EW M+ ++N P +++K  +E+D  VG+DRLV ESDLPNL Y++  + E  RL+  AP  +PH ++ D ++
Sbjct:  336 EWGMAEMINEPSIMQKAVEEIDRVVGKDRLVIESDLPNLNYVKACVKEAFRLHPVAPFNLPHMSTTDTVV 405

[Close]

BLAST of HO064832 vs. SwissProt
Match: C93A3_SOYBN (Cytochrome P450 93A3 OS=Glycine max GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 6.292e-13
Identity = 32/64 (50.00%), Postives = 47/64 (73.44%), Query Frame = -1

Query:   20 EWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSA 211
            EW+M+ L+N+P VL+K R E+D  VG+ R+V+ESD+ NL Y+Q ++ ETLRL+   PLL   S+
Sbjct:  317 EWAMAELINNPGVLEKARQEMDAVVGKSRIVEESDIANLPYLQGIVRETLRLHPAGPLLFRESS 380

[Close]

BLAST of HO064832 vs. TrEMBL
Match: Q9XFX0_CICAR (Cytochrome P450 monooxygenase OS=Cicer arietinum GN=cyp81E3v2 PE=2 SV=1)

HSP 1 Score: 146.362 bits (368), Expect = 8.952e-34
Identity = 73/96 (76.04%), Postives = 76/96 (79.17%), Query Frame = -1

Query:    2 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 289
            YYTDQII GL               LEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLP LTY++NVIYETLRL  PAPLL+PHS SDDCII
Sbjct:  286 YYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPKLTYLKNVIYETLRLCTPAPLLLPHSTSDDCII 381

[Close]

BLAST of HO064832 vs. TrEMBL
Match: Q9ZRW6_CICAR (Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 1.293e-32
Identity = 70/96 (72.92%), Postives = 75/96 (78.12%), Query Frame = -1

Query:    2 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 289
            YYTDQII GL               LEWSMSNLLNHPE+LKKV+DELD HVGQDRLVDESDLP LTY++NVIYETLRL  PAPLL+PHS SDDCII
Sbjct:  286 YYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEILKKVKDELDIHVGQDRLVDESDLPKLTYLKNVIYETLRLCTPAPLLLPHSTSDDCII 381

[Close]

BLAST of HO064832 vs. TrEMBL
Match: Q9ZWF2_GLYEC (Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-31 PE=2 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 1.094e-31
Identity = 69/96 (71.88%), Postives = 75/96 (78.12%), Query Frame = -1

Query:    2 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 289
            YYTDQII GL               LEWSMSNLLNHPEVLKKV+DELDTHVGQDRLVDESDLP L+Y++NVI ETLRLY PAPLL+PHS SD+C I
Sbjct:  286 YYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLSYLKNVINETLRLYTPAPLLLPHSTSDECNI 381

[Close]

BLAST of HO064832 vs. TrEMBL
Match: Q6WNR0_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 3.184e-31
Identity = 66/96 (68.75%), Postives = 76/96 (79.17%), Query Frame = -1

Query:    2 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 289
            YYTD II GL               LEW+MSN+LN+PEVLKKVRDE+DTHVGQDRLVDESDLP LTY++NVIYETLRLY PAPLL+PHS +D+CI+
Sbjct:  286 YYTDTIIKGLCLAMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLRLYTPAPLLLPHSTADECIM 381

[Close]

BLAST of HO064832 vs. TrEMBL
Match: Q9MBE4_LOTJA (Cytochrome P450 OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 6.005e-30
Identity = 65/96 (67.71%), Postives = 74/96 (77.08%), Query Frame = -1

Query:    2 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 289
            YYTDQII GL               LEWSM N+LN+PEVL+K++ ELDTHVGQDRLVDESD+P LTY++NVI ETLRLY PAPLL+PHSASDDC I
Sbjct:  287 YYTDQIIKGLALAMLLAGTDSSAVTLEWSMCNVLNYPEVLEKIKAELDTHVGQDRLVDESDIPKLTYLKNVINETLRLYTPAPLLLPHSASDDCTI 382

[Close]

BLAST of HO064832 vs. TrEMBL
Match: Q2LAK9_SOYBN (Cytochrome P450 monooxygenase CYP81E10 OS=Glycine max GN=CYP82E13 PE=2 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 4.758e-27
Identity = 61/97 (62.89%), Postives = 71/97 (73.20%), Query Frame = -1

Query:    2 RYYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 292
            +YYTDQII GL               LEWS+SNLLNHPEVLKK RDELDT VGQDRL++ESDLP L Y++ +I ETLRLY PAP+LIPH +S+D  I
Sbjct:  289 QYYTDQIIKGLALAMLFGGTDSSTGTLEWSLSNLLNHPEVLKKARDELDTQVGQDRLLNESDLPKLPYLRKIILETLRLYPPAPILIPHVSSEDITI 385

[Close]

BLAST of HO064832 vs. TrEMBL
Match: Q6WNQ8_MEDTR (CYP81E8 OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 1.692e-24
Identity = 51/93 (54.84%), Postives = 68/93 (73.12%), Query Frame = -1

Query:   11 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDD 289
            YYTDQII GL+              +EW+MSNLLNHPE++KK ++ELDTH+G DR VDE D+  L Y+Q+++YETLRL+  APLL+PH +S+D
Sbjct:  286 YYTDQIIKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLHAAAPLLVPHLSSED 378

[Close]

BLAST of HO064832 vs. TrEMBL
Match: D7U6Y4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_705.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00002378001 PE=4 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 1.692e-24
Identity = 55/96 (57.29%), Postives = 69/96 (71.88%), Query Frame = -1

Query:    2 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 289
            YYTD II GLV              +EW+M+ LLNHP+VLKK + ELD HVG+DRL++ESDLP L Y+Q++I ETLRL+  APLL+PH +SDDC I
Sbjct:  102 YYTDDIIKGLVLVLILAGTDTSAATVEWAMTLLLNHPDVLKKAKAELDIHVGKDRLIEESDLPKLRYLQSIISETLRLFPVAPLLVPHMSSDDCQI 197

[Close]

BLAST of HO064832 vs. TrEMBL
Match: D7TTD5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_129.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00000200001 PE=4 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 2.210e-24
Identity = 52/94 (55.32%), Postives = 68/94 (72.34%), Query Frame = -1

Query:    8 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDC 289
            YYTD+II GL+              +EW+MS LLNHPEVLKK RDELDTH+G D L+DE+DLP L Y+Q++I E+LRL+   PLL+PH +++DC
Sbjct:  771 YYTDEIIKGLIVAMQVGGADTTAVTIEWAMSLLLNHPEVLKKARDELDTHIGHDCLIDETDLPKLQYLQSIISESLRLFPSTPLLVPHFSTEDC 864

HSP 2 Score: 105.145 bits (261), Expect = 2.287e-21
Identity = 51/94 (54.26%), Postives = 65/94 (69.15%), Query Frame = -1

Query:    8 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDC 289
            YYTDQII G+               +EW+MS LLNHP+VLKK + ELDT VGQ+RL++E+DLP L Y+QN+I ET RL  PAPL +PH +S +C
Sbjct:  287 YYTDQIIKGVTMNLVFAGTDTAAVTMEWAMSLLLNHPDVLKKAKVELDTCVGQERLLEEADLPKLHYLQNIISETFRLCPPAPLWLPHMSSANC 380

[Close]

BLAST of HO064832 vs. TrEMBL
Match: D7ST38_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_790.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00002607001 PE=4 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 2.210e-24
Identity = 55/96 (57.29%), Postives = 68/96 (70.83%), Query Frame = -1

Query:    2 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 289
            YYTD II GLV              +EW+M+ LLNHP+VLKK + ELD HVG+DRL++ESDLP L Y+Q +I ETLRL+  APLL+PH +SDDC I
Sbjct:  271 YYTDDIIKGLVLILILAGTDTSAATMEWAMTLLLNHPDVLKKAKAELDIHVGKDRLIEESDLPELRYLQRIISETLRLFPVAPLLVPHMSSDDCQI 366

[Close]

BLAST of HO064832 vs. TAIR peptide
Match: AT4G37330.1 (| Symbols: CYP81D4 | cytochrome P450, family 81, subfamily D, polypeptide 4 | chr4:17562547-17564569 REVERSE LENGTH=492)

HSP 1 Score: 112.079 bits (279), Expect = 7.362e-26
Identity = 52/94 (55.32%), Postives = 67/94 (71.28%), Query Frame = -1

Query:    8 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDC 289
            YYTD II G++              LEW+MSNLLNHPE+LKK R E+D  VG DRLVDESD+ NL+Y+Q+++ ETLR+Y   PLL+PH +S+DC
Sbjct:  281 YYTDVIIKGIILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLRMYPAVPLLLPHLSSEDC 374

[Close]

BLAST of HO064832 vs. TAIR peptide
Match: AT1G66540.2 (| Symbols: | Cytochrome P450 superfamily protein | chr1:24825446-24826502 FORWARD LENGTH=327)

HSP 1 Score: 111.694 bits (278), Expect = 9.615e-26
Identity = 51/94 (54.26%), Postives = 67/94 (71.28%), Query Frame = -1

Query:    8 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDC 289
            YYTD  I G +              LEW++S+LLN+PEVLKKVRDE+D  +G DRL++ESD+PNL Y+QN++ ETLRLY   PLL+PH +S+DC
Sbjct:  112 YYTDHTIKGTMLSLILAGTDTSAVTLEWALSSLLNNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVSETLRLYPAGPLLVPHISSEDC 205

[Close]

BLAST of HO064832 vs. TAIR peptide
Match: AT1G66540.1 (| Symbols: | Cytochrome P450 superfamily protein | chr1:24824837-24826502 FORWARD LENGTH=386)

HSP 1 Score: 111.694 bits (278), Expect = 9.615e-26
Identity = 51/94 (54.26%), Postives = 67/94 (71.28%), Query Frame = -1

Query:    8 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDC 289
            YYTD  I G +              LEW++S+LLN+PEVLKKVRDE+D  +G DRL++ESD+PNL Y+QN++ ETLRLY   PLL+PH +S+DC
Sbjct:  171 YYTDHTIKGTMLSLILAGTDTSAVTLEWALSSLLNNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVSETLRLYPAGPLLVPHISSEDC 264

[Close]

BLAST of HO064832 vs. TAIR peptide
Match: AT5G36220.1 (| Symbols: CYP81D1, CYP91A1 | cytochrome p450 81d1 | chr5:14253827-14256015 REVERSE LENGTH=502)

HSP 1 Score: 110.923 bits (276), Expect = 1.640e-25
Identity = 50/96 (52.08%), Postives = 67/96 (69.79%), Query Frame = -1

Query:    2 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 289
            YYTDQII G++              LEW++SNLLNHP+V+ K RDE+D  VG DRL++E+DL  L Y++N++ ETLRL+   PLL+PH AS+DC I
Sbjct:  290 YYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKI 385

[Close]

BLAST of HO064832 vs. TAIR peptide
Match: AT4G37320.1 (| Symbols: CYP81D5 | cytochrome P450, family 81, subfamily D, polypeptide 5 | chr4:17559742-17561690 REVERSE LENGTH=495)

HSP 1 Score: 110.923 bits (276), Expect = 1.640e-25
Identity = 51/94 (54.26%), Postives = 66/94 (70.21%), Query Frame = -1

Query:    8 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDC 289
            YYTD II G++              LEW+MSNLLNHPE+L+K R E+D  +G DRLV+ESD+ NL Y+QN++ ETLRLY   PLL+PH +SD+C
Sbjct:  284 YYTDVIIKGIILALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLRLYPAVPLLLPHFSSDEC 377

[Close]

BLAST of HO064832 vs. TAIR peptide
Match: AT4G37370.1 (| Symbols: CYP81D8 | cytochrome P450, family 81, subfamily D, polypeptide 8 | chr4:17569971-17571678 REVERSE LENGTH=497)

HSP 1 Score: 109.768 bits (273), Expect = 3.654e-25
Identity = 50/94 (53.19%), Postives = 67/94 (71.28%), Query Frame = -1

Query:    8 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDC 289
            Y+TD+II G +              LEW++SN+LNHP+VL K RDE+D  +G DRL+DESD+ NL Y+QN++ ETLRLY  AP+L+PH AS+DC
Sbjct:  283 YFTDRIIKGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPAAPMLLPHVASEDC 376

[Close]

BLAST of HO064832 vs. TAIR peptide
Match: AT3G28740.1 (| Symbols: CYP81D1 | Cytochrome P450 superfamily protein | chr3:10788764-10790552 REVERSE LENGTH=509)

HSP 1 Score: 109.383 bits (272), Expect = 4.772e-25
Identity = 50/96 (52.08%), Postives = 67/96 (69.79%), Query Frame = -1

Query:    2 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 289
            YYTD II G++              LEW+MSNLLNHPEVL+K + E+D  +G DRLV+E D+  L Y+Q+++ ETLRLY  AP+L+PH AS+DCI+
Sbjct:  292 YYTDVIIKGIILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPVAPMLLPHLASEDCIV 387

[Close]

BLAST of HO064832 vs. TAIR peptide
Match: AT4G37360.1 (| Symbols: CYP81D2 | cytochrome P450, family 81, subfamily D, polypeptide 2 | chr4:17567124-17568858 REVERSE LENGTH=499)

HSP 1 Score: 107.457 bits (267), Expect = 1.813e-24
Identity = 50/94 (53.19%), Postives = 66/94 (70.21%), Query Frame = -1

Query:    8 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDC 289
            YY D+II G +              LEW++S+LLN+PEVL K RDE+D  +G DRL++ESD+PNL Y+QN++ ETLRLY  AP+L+PH AS DC
Sbjct:  284 YYMDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLYPAAPMLLPHVASKDC 377

[Close]

BLAST of HO064832 vs. TAIR peptide
Match: AT4G37340.1 (| Symbols: CYP81D3 | cytochrome P450, family 81, subfamily D, polypeptide 3 | chr4:17564953-17566706 REVERSE LENGTH=500)

HSP 1 Score: 106.301 bits (264), Expect = 4.040e-24
Identity = 49/94 (52.13%), Postives = 66/94 (70.21%), Query Frame = -1

Query:    8 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDC 289
            YYTD II G++              LEW++S LLNHP++L K RDE+D  VG +RLV+ESDL +L Y+QN++ E+LRLY  +PLL+PH AS+DC
Sbjct:  284 YYTDNIIKGIMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRLYPASPLLVPHVASEDC 377

[Close]

BLAST of HO064832 vs. TAIR peptide
Match: AT2G23190.1 (| Symbols: CYP81D7 | cytochrome P450, family 81, subfamily D, polypeptide 7 | chr2:9877058-9879007 FORWARD LENGTH=543)

HSP 1 Score: 105.145 bits (261), Expect = 8.999e-24
Identity = 51/96 (53.12%), Postives = 63/96 (65.62%), Query Frame = -1

Query:    2 YYTDQIINGLVXXXXXXXXXXXXXXLEWSMSNLLNHPEVLKKVRDELDTHVGQDRLVDESDLPNLTYIQNVIYETLRLYGPAPLLIPHSASDDCII 289
            YYTD  + G++              LEW+M NLLNHPEVL+K R E+DT VG DRL+DE+D  NL Y+Q ++ ETLRLY  AP  IPH  SDDCI+
Sbjct:  328 YYTDVTLKGIIIVMILAGTETLAGTLEWAMLNLLNHPEVLEKARTEIDTEVGFDRLMDEADTKNLPYLQWIVLETLRLYPVAPTNIPHMTSDDCIL 423

The following BLAST results are available for this feature:

BLAST of HO064832 vs. SwissProt
BLAST of HO064832 vs. TrEMBL
BLAST of HO064832 vs. TAIR peptide

BLAST of HO064832 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
C81E1_GLYEC	1.881e-33	72.92	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata ...	[more]
C81D1_ARATH	2.085e-24	52.08	Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CY...	[more]
C81F1_ARATH	1.826e-20	51.61	Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CY...	[more]
C82A2_SOYBN	1.147e-14	53.62	Cytochrome P450 82A2 OS=Glycine max GN=CYP82A2 PE=...	[more]
C82A1_PEA	1.147e-14	49.28	Cytochrome P450 82A1 (Fragment) OS=Pisum sativum G...	[more]
F3PH_ARATH	3.336e-14	39.36	Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana...	[more]
C98A1_SORBI	9.706e-14	50.77	Cytochrome P450 98A1 OS=Sorghum bicolor GN=CYP98A1...	[more]
C93A1_SOYBN	4.817e-13	48.44	Cytochrome P450 93A1 OS=Glycine max GN=CYP93A1 PE=...	[more]
C79A2_ARATH	4.817e-13	42.86	Phenylalanine N-hydroxylase OS=Arabidopsis thalian...	[more]
C93A3_SOYBN	6.292e-13	50.00	Cytochrome P450 93A3 OS=Glycine max GN=CYP93A3 PE=...	[more]

BLAST of HO064832 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9XFX0_CICAR	8.952e-34	76.04	Cytochrome P450 monooxygenase OS=Cicer arietinum G...	[more]
Q9ZRW6_CICAR	1.293e-32	72.92	Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2...	[more]
Q9ZWF2_GLYEC	1.094e-31	71.88	Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-...	[more]
Q6WNR0_MEDTR	3.184e-31	68.75	Isoflavone 2'-hydroxylase OS=Medicago truncatula P...	[more]
Q9MBE4_LOTJA	6.005e-30	67.71	Cytochrome P450 OS=Lotus japonicus PE=2 SV=1	[more]
Q2LAK9_SOYBN	4.758e-27	62.89	Cytochrome P450 monooxygenase CYP81E10 OS=Glycine ...	[more]
Q6WNQ8_MEDTR	1.692e-24	54.84	CYP81E8 OS=Medicago truncatula PE=2 SV=1	[more]
D7U6Y4_VITVI	1.692e-24	57.29	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7TTD5_VITVI	2.210e-24	55.32	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7ST38_VITVI	2.210e-24	57.29	Whole genome shotgun sequence of line PN40024, sca...	[more]

BLAST of HO064832 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT4G37330.1	7.362e-26	55.32	\| Symbols: CYP81D4 \| cytochrome P450, family 81, s...	[more]
AT1G66540.2	9.615e-26	54.26	\| Symbols: \| Cytochrome P450 superfamily protein ...	[more]
AT1G66540.1	9.615e-26	54.26	\| Symbols: \| Cytochrome P450 superfamily protein ...	[more]
AT5G36220.1	1.640e-25	52.08	\| Symbols: CYP81D1, CYP91A1 \| cytochrome p450 81d1...	[more]
AT4G37320.1	1.640e-25	54.26	\| Symbols: CYP81D5 \| cytochrome P450, family 81, s...	[more]
AT4G37370.1	3.654e-25	53.19	\| Symbols: CYP81D8 \| cytochrome P450, family 81, s...	[more]
AT3G28740.1	4.772e-25	52.08	\| Symbols: CYP81D1 \| Cytochrome P450 superfamily p...	[more]
AT4G37360.1	1.813e-24	53.19	\| Symbols: CYP81D2 \| cytochrome P450, family 81, s...	[more]
AT4G37340.1	4.040e-24	52.13	\| Symbols: CYP81D3 \| cytochrome P450, family 81, s...	[more]
AT2G23190.1	8.999e-24	53.13	\| Symbols: CYP81D7 \| cytochrome P450, family 81, s...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 62..72 score
None	No IPR available	PANTHER	PTHR19383:SF42	CYTOCHROME P450 SUBFAMILY 81D	coord: 2..97 score: 3.9
None	No IPR available	SEG	seg	seg	coord: 13..27 score
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 18..35 score: 6.5e-06coord: 71..82 score: 6.5
IPR001128	Cytochrome P450	GENE3D	1.10.630.10	no description	coord: 3..97 score: 5.6
IPR001128	Cytochrome P450	PANTHER	PTHR19383	CYTOCHROME P450	coord: 2..97 score: 3.9
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 3..96 score: 1.8
IPR001128	Cytochrome P450	SUPERFAMILY	48264	Cytochrome P450	coord: 3..97 score: 1.6
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 7..24 score: 3.6e-16coord: 27..53 score: 3.6e-16coord: 70..88 score: 3.6

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO064832 ID=HO064832; Name=HO064832; organism=Cicer arietinum; type=EST; length=292bp
CAATAATGCAATCATCTGAAGCTGAGTGTGGTATTAATAAAGGGGCTGGA
CCATACAACCGAAGCGTCTCGTAGATCACATTTTGAATGTAAGTCAGATT
TGGAAGGTCTGATTCATCTACCAAACGATCTTGTCCTACATGAGTATCCA
ATTCATCCCTTACCTTCTTCAGTACCTCTGGATGGTTCAACAAATTTGAC
ATTGACCACTCTAAAGTTAAAGCAGTAGTGTCGGTTCCGGCAAGGAGCAT
GGCCAAAACAAGGCCATTGATGATTTGGTCGGTGTAGTACCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004497	monooxygenase activity
GO:0005506	iron ion binding
GO:0009055	electron transfer activity
GO:0020037	heme binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450
IPR002401	Cyt_P450_E_grp-I

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