HO068041

EST Overview


Name	HO068041
Unique Name	HO068041
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	629

Libraries


Library Name	Type
LIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of HO068041 vs. SwissProt
Match: M3K4_HUMAN (Mitogen-activated protein kinase kinase kinase 4 OS=Homo sapiens GN=MAP3K4 PE=1 SV=2)

HSP 1 Score: 85.5001 bits (210), Expect = 2.464e-16
Identity = 40/80 (50.00%), Postives = 54/80 (67.50%), Query Frame = -2

Query:  386 ADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFVE 625
            ADIW+LGC V+EMVTGK  W+    +  + ++ ++G G + P IPE LS EGKDF++ C   DP  RWTA  LL+H FV+
Sbjct: 1526 ADIWSLGCVVIEMVTGKRPWHEYEHN--FQIMYKVGMGHKPP-IPERLSPEGKDFLSHCLESDPKMRWTASQLLDHSFVK 1602

[Close]

BLAST of HO068041 vs. SwissProt
Match: M3K3_ARATH (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana GN=ANP3 PE=1 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 9.364e-16
Identity = 40/79 (50.63%), Postives = 53/79 (67.09%), Query Frame = -2

Query:  389 ADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV 625
            ADIW++GC V+EM TGK  W+ +     ++ +L IG+ +  P IPEDLS E KDF+ KC  K+PS R +A  LL HPFV
Sbjct:  254 ADIWSVGCTVIEMATGKPPWSEQYQQ--FAAVLHIGRTKAHPPIPEDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFV 330

[Close]

BLAST of HO068041 vs. SwissProt
Match: M3K4_MOUSE (Mitogen-activated protein kinase kinase kinase 4 OS=Mus musculus GN=Map3k4 PE=1 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 3.558e-15
Identity = 39/80 (48.75%), Postives = 53/80 (66.25%), Query Frame = -2

Query:  386 ADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFVE 625
            ADIW+LGC V+EMVTGK  W+    +  + ++ ++G G + P IPE LS EGK F++ C   DP  RWTA  LL+H FV+
Sbjct: 1515 ADIWSLGCVVIEMVTGKRPWHEYEHN--FQIMYKVGMGHKPP-IPERLSPEGKAFLSHCLESDPKIRWTASQLLDHAFVK 1591

[Close]

BLAST of HO068041 vs. SwissProt
Match: M3K2_ARATH (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana GN=ANP2 PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 2.550e-13
Identity = 34/79 (43.04%), Postives = 51/79 (64.56%), Query Frame = -2

Query:  389 ADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV 625
            ADIW++GC V+EMVTGK+ W+ +      + +  IG  +  P IP+++S +  DF+ KC  ++P+ R TA  LL HPFV
Sbjct:  254 ADIWSVGCTVIEMVTGKAPWSQQYKEI--AAIFHIGTTKSHPPIPDNISSDANDFLLKCLQQEPNLRPTASELLKHPFV 330

[Close]

BLAST of HO068041 vs. SwissProt
Match: ANP1_ARATH (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana GN=ANP1 PE=1 SV=2)

HSP 1 Score: 74.3294 bits (181), Expect = 5.681e-13
Identity = 36/79 (45.57%), Postives = 50/79 (63.29%), Query Frame = -2

Query:  389 ADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV 625
            ADIW++GC V+EMVTGK+ W+ +      + +  IG  +  P IP+ LS + KDF+ KC  + P+ R TA  LL HPFV
Sbjct:  255 ADIWSVGCTVIEMVTGKAPWSQQYKEV--AAIFFIGTTKSHPPIPDTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFV 331

[Close]

BLAST of HO068041 vs. SwissProt
Match: BCK1_YEAST (Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS=Saccharomyces cerevisiae GN=BCK1 PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.820e-12
Identity = 39/84 (46.43%), Postives = 54/84 (64.29%), Query Frame = -2

Query:  386 DIWALGCAVVEMVTGKSAW-NLENDSCLWSLLLRIGKGEESPVIPED----LSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFVE 622
            DIW+LGC V+EM  GK  W NLE    + + + +IGK + +P IPED    +S+ G++F+  CF  +P KR TA  LL+HPF E
Sbjct: 1362 DIWSLGCIVLEMFAGKRPWSNLE----VVAAMFKIGKSKSAPPIPEDTLPLISQIGRNFLDACFEINPEKRPTANELLSHPFSE 1441

[Close]

BLAST of HO068041 vs. SwissProt
Match: WRK19_ARATH (Probable WRKY transcription factor 19 OS=Arabidopsis thaliana GN=WRKY19 PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.828e-11
Identity = 37/80 (46.25%), Postives = 52/80 (65.00%), Query Frame = -2

Query:  389 PADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV 628
            PADIW+LGC V+EM+TG+  ++   D  + + L  IG G+  P IP+ LS + +DF+  C   +P +R TA  LLNHPFV
Sbjct: 1802 PADIWSLGCTVLEMLTGQIPYS---DLEIGTALYNIGTGK-LPKIPDILSLDARDFILTCLKVNPEERPTAAELLNHPFV 1877

[Close]

BLAST of HO068041 vs. SwissProt
Match: MKKA_DICDI (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum GN=mkkA PE=1 SV=2)

HSP 1 Score: 68.5514 bits (166), Expect = 3.117e-11
Identity = 32/79 (40.51%), Postives = 47/79 (59.49%), Query Frame = -2

Query:  389 ADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV 625
            +DIW+LGC +VEM T +  W+  N + L +++  I      P IP  +S+E  DF+  CF +DP +R  A  LL HPF+
Sbjct:  353 SDIWSLGCVIVEMATAQPPWS--NITELAAVMYHIASSNSIPNIPSHMSQEAFDFLNLCFKRDPKERPDANQLLKHPFI 429

[Close]

BLAST of HO068041 vs. SwissProt
Match: M3K1_ARATH (Mitogen-activated protein kinase kinase kinase 1 OS=Arabidopsis thaliana GN=MEKK1 PE=1 SV=2)

HSP 1 Score: 67.3958 bits (163), Expect = 6.945e-11
Identity = 37/80 (46.25%), Postives = 50/80 (62.50%), Query Frame = -2

Query:  389 PADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV 628
            PADIW+LGC V+EM TG+  ++   D      L RIG+G   P +P+ LS + + F+ KC   +P +R TA  LLNHPFV
Sbjct:  512 PADIWSLGCTVLEMCTGQIPYS---DLEPVQALFRIGRGT-LPEVPDTLSLDARLFILKCLKVNPEERPTAAELLNHPFV 587

[Close]

BLAST of HO068041 vs. SwissProt
Match: SSK22_YEAST (Serine/threonine-protein kinase SSK22 OS=Saccharomyces cerevisiae GN=SSK22 PE=1 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 1.547e-10
Identity = 35/98 (35.71%), Postives = 61/98 (62.24%), Query Frame = -2

Query:  344 DIWALGCAVVEMVTGKSAW-NLENDSCLWSLLLRIGKGEESPVIP--EDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFVEEI--VSMKNVNEDESP 622
            D+WALGC V+EM TG+  W NL+N+   W+++  +  G   P +P  ++++  G+ F+ +C V+DP+ R TA  LL  P++ +I  ++  N  +D+ P
Sbjct: 1234 DVWALGCVVLEMATGRRPWSNLDNE---WAIMYHVAAG-RIPQLPNRDEMTAAGRAFLERCLVQDPTMRATAVELLIDPWMIQIREIAFGNSEKDQVP 1327

[Close]

BLAST of HO068041 vs. TrEMBL
Match: Q9FHA5_ARATH (Protein kinase-like protein OS=Arabidopsis thaliana GN=At5g67080 PE=4 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 8.340e-33
Identity = 78/166 (46.99%), Postives = 100/166 (60.24%), Query Frame = -2

Query:  164 DIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV-------------EEIVSMKNVNEDESPRTHFDFNNWXXXXXXXXXXXXXSDELCPWDIDSVADRLRQLVTIDGPVSWSESDGWTNVR 622
            D+WALGC VVEM +GK+AW+L+  S   SLLLRIG G+E P+IPE+LSE+G+DF++KCFVKDP KRWTAEMLLNHPFV             + +V+MK  +   SPR  F+F +W                     IDS  +R+  LVT D    WS ++ W  VR
Sbjct:  192 DVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIPEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFVVNMKTEDVSTSPRCPFEFPDWVSVSSGSQ------------TIDSPDERVASLVT-DMIPDWSVTNSWVTVR 344

[Close]

BLAST of HO068041 vs. TrEMBL
Match: D7MKT8_ARALY (MAPKKK19 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_920005 PE=4 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 3.169e-32
Identity = 77/166 (46.39%), Postives = 99/166 (59.64%), Query Frame = -2

Query:  164 DIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV-------------EEIVSMKNVNEDESPRTHFDFNNWXXXXXXXXXXXXXSDELCPWDIDSVADRLRQLVTIDGPVSWSESDGWTNVR 622
            D+WALGCAVVEM +GK+AW+L+  S   SLL+RIG G+E P+IPE+LSE+GKDF++KCFVKDP KRWTAEMLL+HPFV             + +V +K      SPR  F+F +W                     IDS  +R+  LVT D    WS ++ W  VR
Sbjct:  192 DVWALGCAVVEMFSGKTAWSLKEGSNFMSLLIRIGVGDELPMIPEELSEQGKDFLSKCFVKDPKKRWTAEMLLHHPFVAVDLDYDGLVEEEDFVVKLKTEEVSTSPRCPFEFPDWVSVSSGSQM------------IDSPEERVTSLVT-DLIPDWSVTNSWVTVR 344

[Close]

BLAST of HO068041 vs. TrEMBL
Match: B9H6H0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_558873 PE=4 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 3.169e-32
Identity = 78/167 (46.71%), Postives = 104/167 (62.28%), Query Frame = -2

Query:  164 DIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFVEE----IVSMKNVNE--DESPRTHFDFNNWXXXXXXXXXXXXXSDE--------LCPWDIDSVADRLRQLVTIDGPVSWSESDGWTNVR 622
            DIWALGCAV+EM+TGK AW+ +  + ++ LL+RIGKG+E P IPE+LS++GKDF++KCFVKDP +RWTA+MLL HPFV +     V ++   E    SPR HFDF +W             SD+        L      S +DR+RQL + D   +WS+S  W  VR
Sbjct:  182 DIWALGCAVIEMLTGKPAWSCKPGTNMFVLLIRIGKGDELPTIPEELSQQGKDFLSKCFVKDPKRRWTADMLLEHPFVADQGKGTVPLREEIEVSSTSPRCHFDFPDWVSIQSPSPRSEFLSDDGVGLVFPSLNSSSWISASDRIRQLAS-DQCCNWSDSGFWVAVR 347

[Close]

BLAST of HO068041 vs. TrEMBL
Match: Q9SND6_ARATH (Protein kinase-like protein OS=Arabidopsis thaliana GN=F11C1_150 PE=1 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 2.271e-30
Identity = 78/165 (47.27%), Postives = 98/165 (59.39%), Query Frame = -2

Query:  164 ADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV--------EEIVSMKNVNEDE---SPRTHFDFNNWXXXXXXXXXXXXXSDELCPWDIDSVADRLRQLVTIDGPVSWSESDGWTNVR 625
            AD+WALGCAVVEM +GK+AW+++  S   SLL+RIG G+E P IPE LSEEGKDF++KCFVKDP+KRWTAEMLLNH FV         E   +K  +ED+   SP+  F+F++W                      DS  +RL  LV+   P  WS    W  VR
Sbjct:  190 ADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCPFEFDDWDSFTLDSNP-----------SFDSPVERLGSLVSGSIP-DWSVGGSWLTVR 342

[Close]

BLAST of HO068041 vs. TrEMBL
Match: Q8LCP0_ARATH (Protein kinase-like protein OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 2.271e-30
Identity = 77/165 (46.67%), Postives = 100/165 (60.61%), Query Frame = -2

Query:  164 ADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV--------EEIVSMKNVNEDE---SPRTHFDFNNWXXXXXXXXXXXXXSDELCPWDIDSVADRLRQLVTIDGPVSWSESDGWTNVR 625
            AD+WALGCAVVEM++GK+AW+++  S   SLL+RIG G+E P IPE LSEEGKDF++KCFVKD +KRWTAEMLLNH FV         E   +K   ED+   SP+  F+F++W                     +DS  +RL  LV+   P  WS  + W +VR
Sbjct:  190 ADVWALGCAVVEMLSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEEGKDFLSKCFVKDQAKRWTAEMLLNHSFVAIDLEDDHREDFVVKVKEEDKVLMSPKCPFEFDDWDSFTLDSNP-----------SLDSPVERLGSLVSGSIP-DWSVGESWVSVR 342

[Close]

BLAST of HO068041 vs. TrEMBL
Match: B9SC81_RICCO (ATP binding protein, putative OS=Ricinus communis GN=RCOM_1409160 PE=4 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 2.271e-30
Identity = 76/177 (42.94%), Postives = 98/177 (55.37%), Query Frame = -2

Query:  164 PADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFVEE----------IVSMKNVNE--DESPRTHFDFNNWXXXXXXXXXXXXXSDELCPWDID----------SVADRLRQLVTIDGPVSWSESDGWTNVR 628
            P DIWALGCAVVEM+TGK AWN   D+ + +LL++IG  +E P IP++LS EGKDF++KCFVKDP +RWTA+MLL+H FV +           V+ KN  E    SPR  F+F  W             S       ID          S  DR++QL +     +W+ SD W  VR
Sbjct:  182 PCDIWALGCAVVEMITGKPAWNCRADANIAALLIKIGVSDELPEIPQELSNEGKDFLSKCFVKDPQRRWTADMLLDHSFVADHYDDEFNSTTTVTFKNKKEVPSSSPRCPFEFQEWISIPSSSPNSEVWSHRELESRIDWSSLSSTPSSSPVDRIQQLTSDHQGCNWAFSDSWITVR 358

[Close]

BLAST of HO068041 vs. TrEMBL
Match: D7LT46_ARALY (MAPKKK20 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_906354 PE=4 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 2.966e-30
Identity = 77/165 (46.67%), Postives = 96/165 (58.18%), Query Frame = -2

Query:  164 ADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV--------EEIVSMKNVNEDE---SPRTHFDFNNWXXXXXXXXXXXXXSDELCPWDIDSVADRLRQLVTIDGPVSWSESDGWTNVR 625
            AD+WALGCAVVEM +GK+AW+++  S   SLL+RIG G+E P IPE LSEEGKDF+ KCFVKDP KRWTAEMLLNH FV         E   +K   E++   SP+  F+F++W                     +DS  +RL  LV+   P  WS    W  VR
Sbjct:  190 ADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEEGKDFLLKCFVKDPEKRWTAEMLLNHSFVAVDLEDYHREDFVVKFKEEEKVLMSPKCPFEFHDWESFTSDSHT-----------SLDSPVERLGSLVSGSSP-DWSVGGSWVTVR 342

[Close]

BLAST of HO068041 vs. TrEMBL
Match: B9H6G9_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_208594 PE=4 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 5.060e-30
Identity = 63/109 (57.80%), Postives = 86/109 (78.90%), Query Frame = -2

Query:  317 ADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV----EEIVSMKNVNED--ESPRTHFDFNNW 625
            ADIWALGCA+VEMVTGK AWN +  + ++ LL+RIG+G+E P+IPE+LS++GKDF++K FVKDP++RWTA+MLL HPFV    +E V +K  +++   SPR HF+F  W
Sbjct:  186 ADIWALGCAIVEMVTGKPAWNCKPGTNMFVLLIRIGEGDELPIIPEELSQQGKDFLSKIFVKDPTQRWTADMLLKHPFVADNFQENVPLKEESKELSTSPRCHFNFPEW 294

[Close]

BLAST of HO068041 vs. TrEMBL
Match: D7SVW7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_31.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00022117001 PE=4 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 1.127e-29
Identity = 80/170 (47.06%), Postives = 99/170 (58.24%), Query Frame = -2

Query:  155 ADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV---EEIVSMKNVNE-DESPRTHFDFNNW---XXXXXXXXXXXXXSDELCPW------DIDSVADRLRQLVTIDGPVSWSESDGWTNVR*NW 625
            ADIWALGCAVVEM +GK AW  +  +   +LL RI  G+ SP  P +LSEEGKDF+ KCF++DP KRWTAEMLL+HPFV   +  V +K  +E   SPR+ FDF  W                  +L  W         S ADR+RQLVT   P +WS S+ W  VR N+
Sbjct:  188 ADIWALGCAVVEMASGKPAWQCQPGTDANALLFRIWGGKASPETPSELSEEGKDFLEKCFLRDPRKRWTAEMLLDHPFVAGTDVTVPLKQSDEASTSPRSPFDFPEWASDHTPSPTPDSEACFDRDLNSWLNSSSHSWTSPADRIRQLVTDQTP-NWSVSESWFAVRNNF 356

[Close]

BLAST of HO068041 vs. TrEMBL
Match: D7MAZ7_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_328051 PE=4 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 2.511e-29
Identity = 77/166 (46.39%), Postives = 101/166 (60.84%), Query Frame = -2

Query:  167 PADIWALGCAVVEMVTGKSAWNLEND--SCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV----EEIVSMKNVNEDE------SPRTHFDFNNWXXXXXXXXXXXXXSDELCPWDIDSVADRLRQLVTIDGPVSWSESDGWTNV 628
            PADIWALGC+VVEM +GK+AW LE+   + + SL++RIG G+E P IP +LSEEGKDFV KCFVK+ ++RWTAEMLL+HPF+    ++  S++   EDE      SPR  FDF  W                +C     S  +R+  LV+   P  WSES  W N+
Sbjct:  185 PADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLMVRIGSGDEVPRIPMELSEEGKDFVRKCFVKNAAERWTAEMLLDHPFLAVDDDKSGSLRCGEEDEACSVSVSPRNPFDFPGWNSVQSPVNESVTFGSLVC-----SPEERISGLVSEKVP-DWSESGDWVNI 344

[Close]

BLAST of HO068041 vs. TAIR peptide
Match: AT5G67080.1 (| Symbols: MAPKKK19 | mitogen-activated protein kinase kinase kinase 19 | chr5:26772726-26773760 FORWARD LENGTH=344)

HSP 1 Score: 144.05 bits (362), Expect = 6.027e-35
Identity = 78/166 (46.99%), Postives = 100/166 (60.24%), Query Frame = -2

Query:  164 DIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV-------------EEIVSMKNVNEDESPRTHFDFNNWXXXXXXXXXXXXXSDELCPWDIDSVADRLRQLVTIDGPVSWSESDGWTNVR 622
            D+WALGC VVEM +GK+AW+L+  S   SLLLRIG G+E P+IPE+LSE+G+DF++KCFVKDP KRWTAEMLLNHPFV             + +V+MK  +   SPR  F+F +W                     IDS  +R+  LVT D    WS ++ W  VR
Sbjct:  192 DVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIPEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFVVNMKTEDVSTSPRCPFEFPDWVSVSSGSQ------------TIDSPDERVASLVT-DMIPDWSVTNSWVTVR 344

[Close]

BLAST of HO068041 vs. TAIR peptide
Match: AT3G50310.1 (| Symbols: MAPKKK20 | mitogen-activated protein kinase kinase kinase 20 | chr3:18648296-18649324 REVERSE LENGTH=342)

HSP 1 Score: 135.961 bits (341), Expect = 1.641e-32
Identity = 78/165 (47.27%), Postives = 98/165 (59.39%), Query Frame = -2

Query:  164 ADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV--------EEIVSMKNVNEDE---SPRTHFDFNNWXXXXXXXXXXXXXSDELCPWDIDSVADRLRQLVTIDGPVSWSESDGWTNVR 625
            AD+WALGCAVVEM +GK+AW+++  S   SLL+RIG G+E P IPE LSEEGKDF++KCFVKDP+KRWTAEMLLNH FV         E   +K  +ED+   SP+  F+F++W                      DS  +RL  LV+   P  WS    W  VR
Sbjct:  190 ADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCPFEFDDWDSFTLDSNP-----------SFDSPVERLGSLVSGSIP-DWSVGGSWLTVR 342

[Close]

BLAST of HO068041 vs. TAIR peptide
Match: AT4G36950.1 (| Symbols: MAPKKK21 | mitogen-activated protein kinase kinase kinase 21 | chr4:17422834-17423844 REVERSE LENGTH=336)

HSP 1 Score: 130.954 bits (328), Expect = 5.280e-31
Identity = 78/161 (48.45%), Postives = 99/161 (61.49%), Query Frame = -2

Query:  164 PADIWALGCAVVEMVTGKSAWNLEND--SCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFVEEIVSMKNVNEDE----SPRTHFDFNNWXXXXXXXXXXXXXSDELCPWDIDSVADRLRQLVTIDGPVSWSESDGWTNVR 628
            PADIWALGC+VVEM +GK+AW LE+   + + SLL+RIG G+E P IP +LSEEGKDFV+KCFVK+ ++RWTAEMLL+HPF+   V  ++  EDE    SPR  FDF  W                     + S  +R+  LV+   P  WS S  W NVR
Sbjct:  184 PADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRIPVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFL--AVDDESGEEDEACSVSPRNPFDFPGWNSVQSPVNDSVMFGSL-----VGSPEERISGLVSEKVP-DWSVSCDWVNVR 336

[Close]

BLAST of HO068041 vs. TAIR peptide
Match: AT5G55090.1 (| Symbols: MAPKKK15 | mitogen-activated protein kinase kinase kinase 15 | chr5:22356852-22358198 REVERSE LENGTH=448)

HSP 1 Score: 103.605 bits (257), Expect = 9.027e-23
Identity = 67/169 (39.64%), Postives = 85/169 (50.30%), Query Frame = -2

Query:  164 PADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFVEE----IVSMKNVNEDESPRTHFDFNNWXXXXXXXXXXXXXSDE-------LCPWDIDSV-ADRLRQLV--TIDGPVSWSESDGWTNVR 628
            PAD+WALGC V+EM TG S W   ND  + + + +IG   ESPVIP  LSE+G+DF+ KC  KDP +RWT E LL HPF++E         N     SP T  D   W               E          WD DS+  DR+++L      G   W E++GW  VR
Sbjct:  179 PADVWALGCTVIEMATGSSPWPELND--VVAAIYKIGFTGESPVIPVWLSEKGQDFLRKCLRKDPKQRWTVEELLQHPFLDEEDNDSDQTGNCLNSSSPSTVLDQRFWDLCETSRSRFIKEDHEDPFANSTNFLWDDDSLPGDRIKKLAGDESSGEPDW-ETNGWIEVR 344

[Close]

BLAST of HO068041 vs. TAIR peptide
Match: AT4G26890.1 (| Symbols: MAPKKK16 | mitogen-activated protein kinase kinase kinase 16 | chr4:13512072-13513406 FORWARD LENGTH=444)

HSP 1 Score: 92.8189 bits (229), Expect = 1.593e-19
Identity = 58/168 (34.52%), Postives = 82/168 (48.81%), Query Frame = -2

Query:  164 PADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFVEEIVSMKN----VNEDESPRTHFDFNNWXXXXXXXXXXXXXSDELCPW-----DIDSVADRLRQLV--TIDGPVSWSESD--GWTNVR 628
            PAD+WALGC ++EM+TG S W   ND  + + + +IG   ESP IP  +S++ KDF+  C  +D  +RWT E LL HPF+++    +      N+  SP T  D   W              D   P+      +DS ADR+ +L        + W   D  GW  VR
Sbjct:  176 PADVWALGCTMIEMMTGSSPWPELND--VVAAMYKIGFSGESPAIPAWISDKAKDFLKNCLKEDQKQRWTVEELLKHPFLDDDEESQTSDCLKNKTSSPSTVLDQRFW-DSCESSKSHLVSIDHEDPFAEYSESLDSPADRIEKLAGDEFSSLLDWDTEDDGGWIQVR 340

[Close]

BLAST of HO068041 vs. TAIR peptide
Match: AT2G30040.1 (| Symbols: MAPKKK14 | mitogen-activated protein kinase kinase kinase 14 | chr2:12821747-12823138 FORWARD LENGTH=463)

HSP 1 Score: 92.4337 bits (228), Expect = 2.081e-19
Identity = 59/154 (38.31%), Postives = 82/154 (53.25%), Query Frame = -2

Query:  164 ADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFVEEIVSMKNVNEDESPRTHFDFNNWXXXXXXXXXXXXXSDELCPWDIDSVADRLRQLVTIDGPVSWSESDGWTNVR 625
            +D+W+LGC V+EM+TGK AW        +  L RIG   + P IP  LSE G+DF+ KC  +D S+RW+ + LL HPF+ +     +   + SPR   D+ N              SDE  P  + S   R+ +L  I G  +W ES+GWT VR
Sbjct:  196 SDVWSLGCTVIEMLTGKPAWEDHG----FDSLSRIGFSNDLPFIPVGLSELGRDFLEKCLKRDRSQRWSCDQLLQHPFLCQ-DHHDSFFTESSPRCVLDWVN------SEFDEEEESDEWRPESMVSAMARISKL-AITGGANW-ESNGWTEVR 336

[Close]

BLAST of HO068041 vs. TAIR peptide
Match: AT1G07150.2 (| Symbols: MAPKKK13 | mitogen-activated protein kinase kinase kinase 13 | chr1:2193983-2195736 REVERSE LENGTH=493)

HSP 1 Score: 92.0485 bits (227), Expect = 2.718e-19
Identity = 55/155 (35.48%), Postives = 79/155 (50.97%), Query Frame = -2

Query:  164 ADIWALGCAVVEMVTGKSAWNLEN-DSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFVEEIVSMKNVNEDESPRTHFDFNNWXXXXXXXXXXXXXSDELCPWDIDSVADRLRQLVTIDGPVSWSESDGWTNVR 625
            +D+W+LGC ++EM TGK AW     DS     L RI   +E PV P  LSE G+DF+ KC  +DP++RW+ + LL HPF+ +  +      + SPR   D+ N               +E+   + +  A  +   +   G V W ESDGW  VR
Sbjct:  191 SDVWSLGCTIIEMFTGKPAWEDHGIDS-----LSRISFSDELPVFPSKLSEIGRDFLEKCLKRDPNQRWSCDQLLQHPFLSQCHNSSPT--ESSPRCVLDWVN------SGFDLEEEEEEVGRSEFEDAAKAIICNLATTGGVIW-ESDGWVEVR 331

[Close]

BLAST of HO068041 vs. TAIR peptide
Match: AT1G07150.1 (| Symbols: MAPKKK13 | mitogen-activated protein kinase kinase kinase 13 | chr1:2194279-2195778 REVERSE LENGTH=499)

HSP 1 Score: 92.0485 bits (227), Expect = 2.718e-19
Identity = 55/155 (35.48%), Postives = 79/155 (50.97%), Query Frame = -2

Query:  164 ADIWALGCAVVEMVTGKSAWNLEN-DSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFVEEIVSMKNVNEDESPRTHFDFNNWXXXXXXXXXXXXXSDELCPWDIDSVADRLRQLVTIDGPVSWSESDGWTNVR 625
            +D+W+LGC ++EM TGK AW     DS     L RI   +E PV P  LSE G+DF+ KC  +DP++RW+ + LL HPF+ +  +      + SPR   D+ N               +E+   + +  A  +   +   G V W ESDGW  VR
Sbjct:  205 SDVWSLGCTIIEMFTGKPAWEDHGIDS-----LSRISFSDELPVFPSKLSEIGRDFLEKCLKRDPNQRWSCDQLLQHPFLSQCHNSSPT--ESSPRCVLDWVN------SGFDLEEEEEEVGRSEFEDAAKAIICNLATTGGVIW-ESDGWVEVR 345

[Close]

BLAST of HO068041 vs. TAIR peptide
Match: AT3G06030.1 (| Symbols: ANP3, MAPKKK12, NP3 | NPK1-related protein kinase 3 | chr3:1818895-1822705 REVERSE LENGTH=651)

HSP 1 Score: 83.5741 bits (205), Expect = 9.667e-17
Identity = 40/79 (50.63%), Postives = 53/79 (67.09%), Query Frame = -2

Query:  389 ADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV 625
            ADIW++GC V+EM TGK  W+ +     ++ +L IG+ +  P IPEDLS E KDF+ KC  K+PS R +A  LL HPFV
Sbjct:  254 ADIWSVGCTVIEMATGKPPWSEQYQQ--FAAVLHIGRTKAHPPIPEDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFV 330

[Close]

BLAST of HO068041 vs. TAIR peptide
Match: AT1G05100.1 (| Symbols: MAPKKK18 | mitogen-activated protein kinase kinase kinase 18 | chr1:1469679-1470698 FORWARD LENGTH=339)

HSP 1 Score: 83.1889 bits (204), Expect = 1.263e-16
Identity = 38/79 (48.10%), Postives = 53/79 (67.09%), Query Frame = -2

Query:  389 ADIWALGCAVVEMVTGKSAWNLENDSCLWSLLLRIGKGEESPVIPEDLSEEGKDFVAKCFVKDPSKRWTAEMLLNHPFV 625
            +DIWA+GC V+EMVTG   W   + +   S+L R+G   E P +P  L+E+ KDF+ KC  K+ ++RWTA  LLNHPF+
Sbjct:  185 SDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAKDFLGKCLKKEATERWTASQLLNHPFL 263

The following BLAST results are available for this feature:

BLAST of HO068041 vs. SwissProt
BLAST of HO068041 vs. TrEMBL
BLAST of HO068041 vs. TAIR peptide

BLAST of HO068041 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
M3K4_HUMAN	2.464e-16	50.00	Mitogen-activated protein kinase kinase kinase 4 O...	[more]
M3K3_ARATH	9.364e-16	50.63	Mitogen-activated protein kinase kinase kinase 3 O...	[more]
M3K4_MOUSE	3.558e-15	48.75	Mitogen-activated protein kinase kinase kinase 4 O...	[more]
M3K2_ARATH	2.550e-13	43.04	Mitogen-activated protein kinase kinase kinase 2 O...	[more]
ANP1_ARATH	5.681e-13	45.57	Mitogen-activated protein kinase kinase kinase ANP...	[more]
BCK1_YEAST	2.820e-12	46.43	Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS...	[more]
WRK19_ARATH	1.828e-11	46.25	Probable WRKY transcription factor 19 OS=Arabidops...	[more]
MKKA_DICDI	3.117e-11	40.51	Mitogen-activated protein kinase kinase kinase A O...	[more]
M3K1_ARATH	6.945e-11	46.25	Mitogen-activated protein kinase kinase kinase 1 O...	[more]
SSK22_YEAST	1.547e-10	35.71	Serine/threonine-protein kinase SSK22 OS=Saccharom...	[more]

BLAST of HO068041 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9FHA5_ARATH	8.340e-33	46.99	Protein kinase-like protein OS=Arabidopsis thalian...	[more]
D7MKT8_ARALY	3.169e-32	46.39	MAPKKK19 OS=Arabidopsis lyrata subsp. lyrata GN=AR...	[more]
B9H6H0_POPTR	3.169e-32	46.71	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q9SND6_ARATH	2.271e-30	47.27	Protein kinase-like protein OS=Arabidopsis thalian...	[more]
Q8LCP0_ARATH	2.271e-30	46.67	Protein kinase-like protein OS=Arabidopsis thalian...	[more]
B9SC81_RICCO	2.271e-30	42.94	ATP binding protein, putative OS=Ricinus communis ...	[more]
D7LT46_ARALY	2.966e-30	46.67	MAPKKK20 OS=Arabidopsis lyrata subsp. lyrata GN=AR...	[more]
B9H6G9_POPTR	5.060e-30	57.80	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
D7SVW7_VITVI	1.127e-29	47.06	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7MAZ7_ARALY	2.511e-29	46.39	Putative uncharacterized protein OS=Arabidopsis ly...	[more]

BLAST of HO068041 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G67080.1	6.027e-35	46.99	\| Symbols: MAPKKK19 \| mitogen-activated protein ki...	[more]
AT3G50310.1	1.641e-32	47.27	\| Symbols: MAPKKK20 \| mitogen-activated protein ki...	[more]
AT4G36950.1	5.280e-31	48.45	\| Symbols: MAPKKK21 \| mitogen-activated protein ki...	[more]
AT5G55090.1	9.027e-23	39.64	\| Symbols: MAPKKK15 \| mitogen-activated protein ki...	[more]
AT4G26890.1	1.593e-19	34.52	\| Symbols: MAPKKK16 \| mitogen-activated protein ki...	[more]
AT2G30040.1	2.081e-19	38.31	\| Symbols: MAPKKK14 \| mitogen-activated protein ki...	[more]
AT1G07150.2	2.718e-19	35.48	\| Symbols: MAPKKK13 \| mitogen-activated protein ki...	[more]
AT1G07150.1	2.718e-19	35.48	\| Symbols: MAPKKK13 \| mitogen-activated protein ki...	[more]
AT3G06030.1	9.667e-17	50.63	\| Symbols: ANP3, MAPKKK12, NP3 \| NPK1-related prot...	[more]
AT1G05100.1	1.263e-16	48.10	\| Symbols: MAPKKK18 \| mitogen-activated protein ki...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 75..90 score
None	No IPR available	GENE3D	1.10.510.10	no description	coord: 2..98 score: 1.5
None	No IPR available	PANTHER	PTHR22986	MAPKK-RELATED SERINE/THREONINE PROTEIN KINASES	coord: 1..114 score: 1.9
None	No IPR available	PANTHER	PTHR22986:SF58	MITOGEN ACTIVATED PROTEIN KINASE-RELATED	coord: 1..114 score: 1.9
None	No IPR available	SEG	seg	seg	coord: 105..118 score
IPR000719	Protein kinase, catalytic domain	PROFILE	PS50011	PROTEIN_KINASE_DOM	coord: 1..80 score: 13
IPR011009	Protein kinase-like domain	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 1..140 score: 2
IPR017442	Serine/threonine-protein kinase-like domain	PFAM	PF00069	Pkinase	coord: 2..80 score: 5.6

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO068041 ID=HO068041; Name=HO068041; organism=Cicer arietinum; type=EST; length=629bp
CTCTTCCATCAAATAGTATCACAAAAATCATGAATCCTACCAAATTTACA
TTCTAAAAATTAGAATTAGAATTAGAATATACAAAATCGAACCAGAACCA
AATTAATCGCAATGATCCATCTTCAACTCCTAATTACTCTGCCTCAACAA
AATTCCAATTTCATCTAACATTAGTCCAACCATCTGATTCCGACCAACTC
ACCGGTCCATCAATCGTCACAAGCTGCCGGAGCCGATCCGCCACCGAATC
AATATCCCATGGACACAACTCATCCGATACCGGTGAACTTATAACGGAAC
TATTAACACTAGGAACCCAATTGTTGAAATCAAAGTGAGTTCTTGGTGAT
TCATCTTCATTAACATTTTTCATTGAAACAATTTCTTCAACAAAAGGATG
ATTCAAAAGCATCTCAGCCGTCCATCTTTTACTCGGATCTTTCACAAAAC
ACTTTGCAACAAAATCTTTTCCCTCTTCCGATAAATCTTCTGGTATCACC
GGAGATTCTTCACCTTTTCCAATTCGAAGCAACAATGACCACAAACACGA
ATCATTCTCCAAATTCCACGCTGATTTTCCCGTTACCATCTCCACCACCG
CACACCCAAGTGCCCAAATATCCGCCGGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004672	protein kinase activity
GO:0005524	ATP binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006468	protein phosphorylation

Vocabulary: **INTERPRO**
Term	Definition
IPR000719	Prot_kinase_dom
IPR011009	Kinase-like_dom_sf
IPR017442	Serine/threonine-protein kinase-like domain

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