FL512458

EST Overview


Name	FL512458
Unique Name	FL512458
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	437

Libraries


Library Name	Type
LIBEST_023567 Subtracted cDNA library of drought-stressed chickpea cultivar	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FL512458 vs. SwissProt
Match: CUL4_ARATH (Cullin-4 OS=Arabidopsis thaliana GN=CUL4 PE=1 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 4.170e-33
Identity = 61/65 (93.85%), Postives = 64/65 (98.46%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            Q+DAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLER+K NPQ+YNYLA
Sbjct:  728 QIDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLEREKSNPQIYNYLA 792

HSP 2 Score: 33.4982 bits (75), Expect = 4.170e-33
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = -1

Query:  369 VNAIQLKETVEENTSTP*KSF 431
            VNAIQ+KETVEENTST  + F
Sbjct:  702 VNAIQMKETVEENTSTTERVF 722

HSP 3 Score: 23.0978 bits (48), Expect = 4.170e-33
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -2

Query:  353 ERVFQDRQY 379
            ERVFQDRQY
Sbjct:  719 ERVFQDRQY 727

[Close]

BLAST of FL512458 vs. SwissProt
Match: CUL4B_MOUSE (Cullin-4B OS=Mus musculus GN=Cul4b PE=1 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 7.458e-26
Identity = 49/65 (75.38%), Postives = 58/65 (89.23%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            Q+DAAIVRIMK RK LSH LL++E++ QLKFP+KPADLKKRIESLIDR+Y+ERDK NP  YNY+A
Sbjct:  906 QIDAAIVRIMKMRKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYNYIA 970

HSP 2 Score: 26.9498 bits (58), Expect = 7.458e-26
Identity = 12/21 (57.14%), Postives = 15/21 (71.43%), Query Frame = -1

Query:  369 VNAIQLKETVEENTSTP*KSF 431
            +N IQ+KETVEE  ST  + F
Sbjct:  880 INQIQMKETVEEQASTTERVF 900

HSP 3 Score: 23.0978 bits (48), Expect = 7.458e-26
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -2

Query:  353 ERVFQDRQY 379
            ERVFQDRQY
Sbjct:  897 ERVFQDRQY 905

[Close]

BLAST of FL512458 vs. SwissProt
Match: CUL4B_HUMAN (Cullin-4B OS=Homo sapiens GN=CUL4B PE=1 SV=4)

HSP 1 Score: 106.686 bits (265), Expect = 7.471e-26
Identity = 49/65 (75.38%), Postives = 58/65 (89.23%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            Q+DAAIVRIMK RK LSH LL++E++ QLKFP+KPADLKKRIESLIDR+Y+ERDK NP  YNY+A
Sbjct:  849 QIDAAIVRIMKMRKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYNYIA 913

HSP 2 Score: 26.9498 bits (58), Expect = 7.471e-26
Identity = 12/21 (57.14%), Postives = 15/21 (71.43%), Query Frame = -1

Query:  369 VNAIQLKETVEENTSTP*KSF 431
            +N IQ+KETVEE  ST  + F
Sbjct:  823 INQIQMKETVEEQASTTERVF 843

HSP 3 Score: 23.0978 bits (48), Expect = 7.471e-26
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -2

Query:  353 ERVFQDRQY 379
            ERVFQDRQY
Sbjct:  840 ERVFQDRQY 848

[Close]

BLAST of FL512458 vs. SwissProt
Match: CUL4A_HUMAN (Cullin-4A OS=Homo sapiens GN=CUL4A PE=1 SV=3)

HSP 1 Score: 102.834 bits (255), Expect = 1.014e-24
Identity = 47/65 (72.31%), Postives = 56/65 (86.15%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            Q+DAAIVRIMK RK L H LL++EL+ QLKFP+KP DLKKRIESLIDR+Y+ERDK NP  Y+Y+A
Sbjct:  695 QIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDNPNQYHYVA 759

HSP 2 Score: 26.9498 bits (58), Expect = 1.014e-24
Identity = 12/21 (57.14%), Postives = 15/21 (71.43%), Query Frame = -1

Query:  369 VNAIQLKETVEENTSTP*KSF 431
            +N IQ+KETVEE  ST  + F
Sbjct:  669 INQIQMKETVEEQVSTTERVF 689

HSP 3 Score: 23.0978 bits (48), Expect = 1.014e-24
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -2

Query:  353 ERVFQDRQY 379
            ERVFQDRQY
Sbjct:  686 ERVFQDRQY 694

[Close]

BLAST of FL512458 vs. SwissProt
Match: CUL4A_MOUSE (Cullin-4A OS=Mus musculus GN=Cul4a PE=1 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 3.724e-24
Identity = 46/65 (70.77%), Postives = 56/65 (86.15%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            Q+DAAIVRIMK RK L H LL++EL+ QLKFP+KP DLKKRIESLIDR+Y+ERDK +P  Y+Y+A
Sbjct:  695 QIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDSPNQYHYVA 759

HSP 2 Score: 26.9498 bits (58), Expect = 3.724e-24
Identity = 12/21 (57.14%), Postives = 15/21 (71.43%), Query Frame = -1

Query:  369 VNAIQLKETVEENTSTP*KSF 431
            +N IQ+KETVEE  ST  + F
Sbjct:  669 INQIQMKETVEEQVSTTERVF 689

HSP 3 Score: 23.0978 bits (48), Expect = 3.724e-24
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -2

Query:  353 ERVFQDRQY 379
            ERVFQDRQY
Sbjct:  686 ERVFQDRQY 694

[Close]

BLAST of FL512458 vs. SwissProt
Match: CUL4_DICDI (Cullin-4 OS=Dictyostelium discoideum GN=culD PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.846e-19
Identity = 45/65 (69.23%), Postives = 51/65 (78.46%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            QVDAAIVRIMKTRK L+H LLI+EL   LKF  KP DLKKRIE LI++EYL RD  N  +YNY+A
Sbjct:  738 QVDAAIVRIMKTRKTLAHNLLISELVSLLKFQPKPVDLKKRIEILIEKEYLCRDPENAMIYNYMA 802

HSP 2 Score: 26.5646 bits (57), Expect = 1.846e-19
Identity = 11/16 (68.75%), Postives = 14/16 (87.50%), Query Frame = -1

Query:  384 VNAIQLKETVEENTST 431
            VN+IQ++ETVEEN  T
Sbjct:  712 VNSIQIQETVEENQKT 727

[Close]

BLAST of FL512458 vs. SwissProt
Match: CUL4_CAEEL (Cullin-4 OS=Caenorhabditis elegans GN=cul-4 PE=1 SV=3)

HSP 1 Score: 87.0409 bits (214), Expect = 3.490e-17
Identity = 42/80 (52.50%), Postives = 56/80 (70.00%), Query Frame = -3

Query:  157 EHKHPLKEFSKIGNIQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 396
            E K   +E +     ++DAA+VRIMK RK L+H  L+TEL QQL+FP+  AD+KKR+ESLI+REY+ RD      YNY+A
Sbjct:  761 ETKEVKEEVNSDRQYKIDAAVVRIMKARKQLNHQTLMTELLQQLRFPVSTADIKKRLESLIEREYISRDPEEASSYNYVA 840

[Close]

BLAST of FL512458 vs. SwissProt
Match: CUL1_ARATH (Cullin-1 OS=Arabidopsis thaliana GN=CUL1 PE=1 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 3.612e-14
Identity = 41/82 (50.00%), Postives = 54/82 (65.85%), Query Frame = -3

Query:  157 EHKHPLKEFSKIGNIQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKP--ADLKKRIESLIDREYLERDKGNPQVYNYLA 396
            E K  +++  K     +DAAIVRIMK+RKVL H  L++E  +QL    KP    +KKR+E LI R+YLERDK NP ++ YLA
Sbjct:  657 ERKKVVEDVDKDRRYAIDAAIVRIMKSRKVLGHQQLVSECVEQLSRMFKPDIKAIKKRMEDLITRDYLERDKENPNMFRYLA 738

[Close]

BLAST of FL512458 vs. SwissProt
Match: CUL3_DICDI (Cullin-3 OS=Dictyostelium discoideum GN=culC PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.793e-13
Identity = 37/67 (55.22%), Postives = 52/67 (77.61%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQL--KFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            Q++A+IVRIMK RK L H+ L++E+ +QL  +F   P  +KKRIESLI+REYLER K + ++YNY+A
Sbjct:  703 QIEASIVRIMKARKTLEHSNLVSEVIKQLQSRFVPNPVIVKKRIESLIEREYLERSKQDRKIYNYMA 769

[Close]

BLAST of FL512458 vs. SwissProt
Match: CUL3A_ARATH (Cullin-3A OS=Arabidopsis thaliana GN=CUL3A PE=1 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 3.993e-13
Identity = 36/67 (53.73%), Postives = 52/67 (77.61%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQL--KFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            Q++AAIVRIMK+RK+L H  +I E+ +QL  +F   P ++KKRIESLI+R++LERD  + ++Y YLA
Sbjct:  666 QIEAAIVRIMKSRKILDHNNIIAEVTKQLQPRFLANPTEIKKRIESLIERDFLERDSTDRKLYRYLA 732

[Close]

BLAST of FL512458 vs. TrEMBL
Match: A9TCM3_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_193769 PE=3 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 1.602e-32
Identity = 62/65 (95.38%), Postives = 64/65 (98.46%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            Q+DAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDK NPQ+YNYLA
Sbjct:  704 QIDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKANPQIYNYLA 768

HSP 2 Score: 34.2686 bits (77), Expect = 1.602e-32
Identity = 17/21 (80.95%), Postives = 18/21 (85.71%), Query Frame = -1

Query:  369 VNAIQLKETVEENTSTP*KSF 431
            VNAIQLKETVEENTST  + F
Sbjct:  678 VNAIQLKETVEENTSTTERVF 698

HSP 3 Score: 23.0978 bits (48), Expect = 1.602e-32
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -2

Query:  353 ERVFQDRQY 379
            ERVFQDRQY
Sbjct:  695 ERVFQDRQY 703

[Close]

BLAST of FL512458 vs. TrEMBL
Match: B9N9Q9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_828634 PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 2.078e-32
Identity = 63/65 (96.92%), Postives = 64/65 (98.46%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDK NPQ+YNYLA
Sbjct:  747 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 811

HSP 2 Score: 33.4982 bits (75), Expect = 2.078e-32
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = -1

Query:  369 VNAIQLKETVEENTSTP*KSF 431
            VNAIQ+KETVEENTST  + F
Sbjct:  721 VNAIQMKETVEENTSTTERVF 741

HSP 3 Score: 23.0978 bits (48), Expect = 2.078e-32
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -2

Query:  353 ERVFQDRQY 379
            ERVFQDRQY
Sbjct:  738 ERVFQDRQY 746

[Close]

BLAST of FL512458 vs. TrEMBL
Match: A5AQK8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_010491 PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 2.079e-32
Identity = 63/65 (96.92%), Postives = 64/65 (98.46%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDK NPQ+YNYLA
Sbjct:  742 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 806

HSP 2 Score: 33.4982 bits (75), Expect = 2.079e-32
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = -1

Query:  369 VNAIQLKETVEENTSTP*KSF 431
            VNAIQ+KETVEENTST  + F
Sbjct:  716 VNAIQMKETVEENTSTTERVF 736

HSP 3 Score: 23.0978 bits (48), Expect = 2.079e-32
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -2

Query:  353 ERVFQDRQY 379
            ERVFQDRQY
Sbjct:  733 ERVFQDRQY 741

[Close]

BLAST of FL512458 vs. TrEMBL
Match: D7TK89_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_3.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00021502001 PE=4 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 2.079e-32
Identity = 63/65 (96.92%), Postives = 64/65 (98.46%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDK NPQ+YNYLA
Sbjct:  738 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 802

HSP 2 Score: 33.4982 bits (75), Expect = 2.079e-32
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = -1

Query:  369 VNAIQLKETVEENTSTP*KSF 431
            VNAIQ+KETVEENTST  + F
Sbjct:  712 VNAIQMKETVEENTSTTERVF 732

HSP 3 Score: 23.0978 bits (48), Expect = 2.079e-32
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -2

Query:  353 ERVFQDRQY 379
            ERVFQDRQY
Sbjct:  729 ERVFQDRQY 737

[Close]

BLAST of FL512458 vs. TrEMBL
Match: A9LK40_SOLLC (Cullin 4 OS=Solanum lycopersicum GN=CUL4 PE=2 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 2.080e-32
Identity = 63/65 (96.92%), Postives = 64/65 (98.46%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDK NPQ+YNYLA
Sbjct:  721 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKNNPQIYNYLA 785

HSP 2 Score: 33.4982 bits (75), Expect = 2.080e-32
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = -1

Query:  369 VNAIQLKETVEENTSTP*KSF 431
            VNAIQ+KETVEENTST  + F
Sbjct:  695 VNAIQMKETVEENTSTTERVF 715

HSP 3 Score: 23.0978 bits (48), Expect = 2.080e-32
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -2

Query:  353 ERVFQDRQY 379
            ERVFQDRQY
Sbjct:  712 ERVFQDRQY 720

[Close]

BLAST of FL512458 vs. TrEMBL
Match: A9SEG5_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_128517 PE=3 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 3.514e-32
Identity = 62/65 (95.38%), Postives = 64/65 (98.46%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            Q+DAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDK NPQ+YNYLA
Sbjct:  681 QIDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKANPQIYNYLA 745

HSP 2 Score: 33.113 bits (74), Expect = 3.514e-32
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = -1

Query:  369 VNAIQLKETVEENTSTP*KSF 431
            VNAIQLKETVEENT+T  + F
Sbjct:  655 VNAIQLKETVEENTTTTERVF 675

HSP 3 Score: 23.0978 bits (48), Expect = 3.514e-32
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -2

Query:  353 ERVFQDRQY 379
            ERVFQDRQY
Sbjct:  672 ERVFQDRQY 680

[Close]

BLAST of FL512458 vs. TrEMBL
Match: B9H410_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_714526 PE=3 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 4.559e-32
Identity = 62/65 (95.38%), Postives = 64/65 (98.46%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDR+YLERDK NPQ+YNYLA
Sbjct:  721 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDRDYLERDKSNPQIYNYLA 785

HSP 2 Score: 33.4982 bits (75), Expect = 4.559e-32
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = -1

Query:  369 VNAIQLKETVEENTSTP*KSF 431
            VNAIQ+KETVEENTST  + F
Sbjct:  695 VNAIQMKETVEENTSTTERVF 715

HSP 3 Score: 23.0978 bits (48), Expect = 4.559e-32
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -2

Query:  353 ERVFQDRQY 379
            ERVFQDRQY
Sbjct:  712 ERVFQDRQY 720

[Close]

BLAST of FL512458 vs. TrEMBL
Match: D7MRZ6_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_916814 PE=4 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 7.692e-32
Identity = 61/65 (93.85%), Postives = 64/65 (98.46%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            Q+DAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLER+K NPQ+YNYLA
Sbjct:  727 QIDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLEREKSNPQIYNYLA 791

HSP 2 Score: 33.4982 bits (75), Expect = 7.692e-32
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = -1

Query:  369 VNAIQLKETVEENTSTP*KSF 431
            VNAIQ+KETVEENTST  + F
Sbjct:  701 VNAIQMKETVEENTSTTERVF 721

HSP 3 Score: 23.0978 bits (48), Expect = 7.692e-32
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -2

Query:  353 ERVFQDRQY 379
            ERVFQDRQY
Sbjct:  718 ERVFQDRQY 726

[Close]

BLAST of FL512458 vs. TrEMBL
Match: C5WY66_SORBI (Putative uncharacterized protein Sb01g005920 OS=Sorghum bicolor GN=Sb01g005920 PE=3 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 2.187e-31
Identity = 59/65 (90.77%), Postives = 64/65 (98.46%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            QVDAAIVRIMKTRKVLSHTLLITEL+QQLKFP+KPAD+KKRIESLIDREYLERD+ NPQ+YNYLA
Sbjct:  770 QVDAAIVRIMKTRKVLSHTLLITELYQQLKFPVKPADIKKRIESLIDREYLERDRSNPQIYNYLA 834

HSP 2 Score: 33.4982 bits (75), Expect = 2.187e-31
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = -1

Query:  369 VNAIQLKETVEENTSTP*KSF 431
            VNAIQ+KETVEENTST  + F
Sbjct:  744 VNAIQMKETVEENTSTTERVF 764

HSP 3 Score: 23.0978 bits (48), Expect = 2.187e-31
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -2

Query:  353 ERVFQDRQY 379
            ERVFQDRQY
Sbjct:  761 ERVFQDRQY 769

[Close]

BLAST of FL512458 vs. TrEMBL
Match: B4FCU9_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 2.307e-31
Identity = 59/65 (90.77%), Postives = 64/65 (98.46%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            QVDAAIVRIMKTRKVLSHTLLITEL+QQLKFP+KPAD+KKRIESLIDREYLERD+ NPQ+YNYLA
Sbjct:   96 QVDAAIVRIMKTRKVLSHTLLITELYQQLKFPVKPADIKKRIESLIDREYLERDRSNPQIYNYLA 160

HSP 2 Score: 33.4982 bits (75), Expect = 2.307e-31
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = -1

Query:  369 VNAIQLKETVEENTSTP*KSF 431
            VNAIQ+KETVEENTST  + F
Sbjct:   70 VNAIQMKETVEENTSTTERVF 90

HSP 3 Score: 23.0978 bits (48), Expect = 2.307e-31
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -2

Query:  353 ERVFQDRQY 379
            ERVFQDRQY
Sbjct:   87 ERVFQDRQY 95

[Close]

BLAST of FL512458 vs. TAIR peptide
Match: AT5G46210.1 (| Symbols: CUL4, ATCUL4 | cullin4 | chr5:18731569-18736653 REVERSE LENGTH=792)

HSP 1 Score: 124.79 bits (312), Expect = 5.036e-34
Identity = 61/65 (93.85%), Postives = 64/65 (98.46%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            Q+DAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLER+K NPQ+YNYLA
Sbjct:  728 QIDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLKKRIESLIDREYLEREKSNPQIYNYLA 792

HSP 2 Score: 33.4982 bits (75), Expect = 5.036e-34
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = -1

Query:  369 VNAIQLKETVEENTSTP*KSF 431
            VNAIQ+KETVEENTST  + F
Sbjct:  702 VNAIQMKETVEENTSTTERVF 722

HSP 3 Score: 23.0978 bits (48), Expect = 5.036e-34
Identity = 9/9 (100.00%), Postives = 9/9 (100.00%), Query Frame = -2

Query:  353 ERVFQDRQY 379
            ERVFQDRQY
Sbjct:  719 ERVFQDRQY 727

[Close]

BLAST of FL512458 vs. TAIR peptide
Match: AT4G02570.4 (| Symbols: CUL1 | cullin 1 | chr4:1129315-1133435 FORWARD LENGTH=738)

HSP 1 Score: 77.0258 bits (188), Expect = 4.393e-15
Identity = 41/82 (50.00%), Postives = 54/82 (65.85%), Query Frame = -3

Query:  157 EHKHPLKEFSKIGNIQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKP--ADLKKRIESLIDREYLERDKGNPQVYNYLA 396
            E K  +++  K     +DAAIVRIMK+RKVL H  L++E  +QL    KP    +KKR+E LI R+YLERDK NP ++ YLA
Sbjct:  657 ERKKVVEDVDKDRRYAIDAAIVRIMKSRKVLGHQQLVSECVEQLSRMFKPDIKAIKKRMEDLITRDYLERDKENPNMFRYLA 738

[Close]

BLAST of FL512458 vs. TAIR peptide
Match: AT4G02570.3 (| Symbols: ATCUL1, CUL1, AXR6 | cullin 1 | chr4:1129315-1133435 FORWARD LENGTH=738)

HSP 1 Score: 77.0258 bits (188), Expect = 4.393e-15
Identity = 41/82 (50.00%), Postives = 54/82 (65.85%), Query Frame = -3

Query:  157 EHKHPLKEFSKIGNIQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKP--ADLKKRIESLIDREYLERDKGNPQVYNYLA 396
            E K  +++  K     +DAAIVRIMK+RKVL H  L++E  +QL    KP    +KKR+E LI R+YLERDK NP ++ YLA
Sbjct:  657 ERKKVVEDVDKDRRYAIDAAIVRIMKSRKVLGHQQLVSECVEQLSRMFKPDIKAIKKRMEDLITRDYLERDKENPNMFRYLA 738

[Close]

BLAST of FL512458 vs. TAIR peptide
Match: AT4G02570.2 (| Symbols: ATCUL1, CUL1, AXR6 | cullin 1 | chr4:1129315-1133435 FORWARD LENGTH=738)

HSP 1 Score: 77.0258 bits (188), Expect = 4.393e-15
Identity = 41/82 (50.00%), Postives = 54/82 (65.85%), Query Frame = -3

Query:  157 EHKHPLKEFSKIGNIQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKP--ADLKKRIESLIDREYLERDKGNPQVYNYLA 396
            E K  +++  K     +DAAIVRIMK+RKVL H  L++E  +QL    KP    +KKR+E LI R+YLERDK NP ++ YLA
Sbjct:  657 ERKKVVEDVDKDRRYAIDAAIVRIMKSRKVLGHQQLVSECVEQLSRMFKPDIKAIKKRMEDLITRDYLERDKENPNMFRYLA 738

[Close]

BLAST of FL512458 vs. TAIR peptide
Match: AT4G02570.1 (| Symbols: ATCUL1, CUL1, AXR6 | cullin 1 | chr4:1129315-1133435 FORWARD LENGTH=738)

HSP 1 Score: 77.0258 bits (188), Expect = 4.393e-15
Identity = 41/82 (50.00%), Postives = 54/82 (65.85%), Query Frame = -3

Query:  157 EHKHPLKEFSKIGNIQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKP--ADLKKRIESLIDREYLERDKGNPQVYNYLA 396
            E K  +++  K     +DAAIVRIMK+RKVL H  L++E  +QL    KP    +KKR+E LI R+YLERDK NP ++ YLA
Sbjct:  657 ERKKVVEDVDKDRRYAIDAAIVRIMKSRKVLGHQQLVSECVEQLSRMFKPDIKAIKKRMEDLITRDYLERDKENPNMFRYLA 738

[Close]

BLAST of FL512458 vs. TAIR peptide
Match: AT1G26830.1 (| Symbols: CUL3A, ATCUL3A, ATCUL3, CUL3 | cullin 3 | chr1:9296063-9298374 FORWARD LENGTH=732)

HSP 1 Score: 73.559 bits (179), Expect = 4.857e-14
Identity = 36/67 (53.73%), Postives = 52/67 (77.61%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQL--KFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            Q++AAIVRIMK+RK+L H  +I E+ +QL  +F   P ++KKRIESLI+R++LERD  + ++Y YLA
Sbjct:  666 QIEAAIVRIMKSRKILDHNNIIAEVTKQLQPRFLANPTEIKKRIESLIERDFLERDSTDRKLYRYLA 732

[Close]

BLAST of FL512458 vs. TAIR peptide
Match: AT1G69670.1 (| Symbols: CUL3B, ATCUL3B | cullin 3B | chr1:26202169-26204442 REVERSE LENGTH=732)

HSP 1 Score: 72.7886 bits (177), Expect = 8.284e-14
Identity = 36/67 (53.73%), Postives = 52/67 (77.61%), Query Frame = -3

Query:  157 QVDAAIVRIMKTRKVLSHTLLITELFQQL--KFPIKPADLKKRIESLIDREYLERDKGNPQVYNYLA 351
            Q++AAIVRIMK+R+VL H  +I E+ +QL  +F   P ++KKRIESLI+R++LERD  + ++Y YLA
Sbjct:  666 QIEAAIVRIMKSRRVLDHNNIIAEVTKQLQTRFLANPTEIKKRIESLIERDFLERDNTDRKLYRYLA 732

[Close]

BLAST of FL512458 vs. TAIR peptide
Match: AT1G02980.1 (| Symbols: CUL2, ATCUL2 | cullin 2 | chr1:677869-681212 FORWARD LENGTH=742)

HSP 1 Score: 72.7886 bits (177), Expect = 8.284e-14
Identity = 39/82 (47.56%), Postives = 51/82 (62.20%), Query Frame = -3

Query:  157 EHKHPLKEFSKIGNIQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKP--ADLKKRIESLIDREYLERDKGNPQVYNYLA 396
            E K  +++  K     +DAA+VRIMK+RKVL H  L++E  + L    KP    +KKRIE LI R+YLERD  NP  + YLA
Sbjct:  661 ERKKIVEDVDKDRRYAIDAALVRIMKSRKVLGHQQLVSECVEHLSKMFKPDIKMIKKRIEDLISRDYLERDTDNPNTFKYLA 742

[Close]

BLAST of FL512458 vs. TAIR peptide
Match: AT1G43140.1 (| Symbols: | Cullin family protein | chr1:16232785-16236109 FORWARD LENGTH=721)

HSP 1 Score: 69.3218 bits (168), Expect = 9.159e-13
Identity = 37/82 (45.12%), Postives = 52/82 (63.41%), Query Frame = -3

Query:  157 EHKHPLKEFSKIGNIQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKP--ADLKKRIESLIDREYLERDKGNPQVYNYLA 396
            E K  +++  K     +DAA+VRIMK+RKVL+H  L++E  + L    KP    +KKRIE LI+R+YLERD  N   + Y+A
Sbjct:  640 ERKKVVEDVDKDRRYAIDAALVRIMKSRKVLAHQQLVSECVEHLSKMFKPDIKMIKKRIEDLINRDYLERDTENANTFKYVA 721

The following BLAST results are available for this feature:

BLAST of FL512458 vs. SwissProt
BLAST of FL512458 vs. TrEMBL
BLAST of FL512458 vs. TAIR peptide

BLAST of FL512458 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
CUL4_ARATH	4.170e-33	93.85	Cullin-4 OS=Arabidopsis thaliana GN=CUL4 PE=1 SV=1	[more]
CUL4B_MOUSE	7.458e-26	75.38	Cullin-4B OS=Mus musculus GN=Cul4b PE=1 SV=1	[more]
CUL4B_HUMAN	7.471e-26	75.38	Cullin-4B OS=Homo sapiens GN=CUL4B PE=1 SV=4	[more]
CUL4A_HUMAN	1.014e-24	72.31	Cullin-4A OS=Homo sapiens GN=CUL4A PE=1 SV=3	[more]
CUL4A_MOUSE	3.724e-24	70.77	Cullin-4A OS=Mus musculus GN=Cul4a PE=1 SV=1	[more]
CUL4_DICDI	1.846e-19	69.23	Cullin-4 OS=Dictyostelium discoideum GN=culD PE=3 ...	[more]
CUL4_CAEEL	3.490e-17	52.50	Cullin-4 OS=Caenorhabditis elegans GN=cul-4 PE=1 S...	[more]
CUL1_ARATH	3.612e-14	50.00	Cullin-1 OS=Arabidopsis thaliana GN=CUL1 PE=1 SV=1	[more]
CUL3_DICDI	1.793e-13	55.22	Cullin-3 OS=Dictyostelium discoideum GN=culC PE=3 ...	[more]
CUL3A_ARATH	3.993e-13	53.73	Cullin-3A OS=Arabidopsis thaliana GN=CUL3A PE=1 SV...	[more]

BLAST of FL512458 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A9TCM3_PHYPA	1.602e-32	95.38	Predicted protein OS=Physcomitrella patens subsp. ...	[more]
B9N9Q9_POPTR	2.078e-32	96.92	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A5AQK8_VITVI	2.079e-32	96.92	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7TK89_VITVI	2.079e-32	96.92	Whole genome shotgun sequence of line PN40024, sca...	[more]
A9LK40_SOLLC	2.080e-32	96.92	Cullin 4 OS=Solanum lycopersicum GN=CUL4 PE=2 SV=1	[more]
A9SEG5_PHYPA	3.514e-32	95.38	Predicted protein OS=Physcomitrella patens subsp. ...	[more]
B9H410_POPTR	4.559e-32	95.38	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7MRZ6_ARALY	7.692e-32	93.85	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
C5WY66_SORBI	2.187e-31	90.77	Putative uncharacterized protein Sb01g005920 OS=So...	[more]
B4FCU9_MAIZE	2.307e-31	90.77	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]

BLAST of FL512458 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 9


Match Name	E-value	Identity	Description
AT5G46210.1	5.036e-34	93.85	\| Symbols: CUL4, ATCUL4 \| cullin4 \| chr5:18731569-...	[more]
AT4G02570.4	4.393e-15	50.00	\| Symbols: CUL1 \| cullin 1 \| chr4:1129315-1133435 ...	[more]
AT4G02570.3	4.393e-15	50.00	\| Symbols: ATCUL1, CUL1, AXR6 \| cullin 1 \| chr4:11...	[more]
AT4G02570.2	4.393e-15	50.00	\| Symbols: ATCUL1, CUL1, AXR6 \| cullin 1 \| chr4:11...	[more]
AT4G02570.1	4.393e-15	50.00	\| Symbols: ATCUL1, CUL1, AXR6 \| cullin 1 \| chr4:11...	[more]
AT1G26830.1	4.857e-14	53.73	\| Symbols: CUL3A, ATCUL3A, ATCUL3, CUL3 \| cullin 3...	[more]
AT1G69670.1	8.284e-14	53.73	\| Symbols: CUL3B, ATCUL3B \| cullin 3B \| chr1:26202...	[more]
AT1G02980.1	8.284e-14	47.56	\| Symbols: CUL2, ATCUL2 \| cullin 2 \| chr1:677869-6...	[more]
AT1G43140.1	9.159e-13	45.12	\| Symbols: \| Cullin family protein \| chr1:1623278...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011991	Winged helix-turn-helix transcription repressor DNA-binding	GENE3D	1.10.10.10	no description	coord: 16..93 score: 9
IPR016157	Cullin, conserved site	PROSITE	PS01256	CULLIN_1	coord: 66..93 score
IPR019559	Cullin protein, neddylation domain	PFAM	PF10557	Cullin_Nedd8	coord: 28..86 score: 4.1
IPR019559	Cullin protein, neddylation domain	SMART	SM00884	no description	coord: 22..87 score: 7.1
None	No IPR available	PANTHER	PTHR11932	CULLIN	coord: 29..93 score: 1.1
None	No IPR available	PANTHER	PTHR11932:SF22	CULLIN 4	coord: 29..93 score: 1.1
None	No IPR available	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 4..93 score: 1.3
None	No IPR available	SEG	seg	seg	coord: 3..17 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FL512458 ID=FL512458; Name=FL512458; organism=Cicer arietinum; type=EST; length=437bp
TTGTCGATAAATGAAAGGGTAAAAAAGGGGAGGGTGGGATCCATTGTTAC
TGTACATATATATGACTAGGGAATAAACATCAAGCTTTTCCCGTGTCATT
TTAAAATTTAAAAACAAAAAGAAACTTTGAAATGTAAAATGAGGTAAAAT
ATACTATGCCAGATAATTGTATACTTGAGGGTTGCCCTTGTCCCTTTCCA
GATACTCTCTGTCAATCAGGCTTTCAATCCTTTTCTTCAAATCAGCTGGT
TTAATAGGAAATTTGAGCTGTTGGAAGAGTTCAGTAATAAGAAGTGTGTG
ACTAAGCACTTTCCTAGTCTTCATTATCCTCACAATAGCAGCATCAACCT
GAATATTGCCGATCTTGGAAAACTCTTTCAGGGGGTGCTTGTGTTCTCCT
CCACTGTTTCCTTCAACTGAATTGCATTCACCACCTG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006511	ubiquitin-dependent protein catabolic process

Vocabulary: **Cellular Component**
Term	Definition
GO:0031461	cullin-RING ubiquitin ligase complex

Vocabulary: **Molecular Function**
Term	Definition
GO:0031625	ubiquitin protein ligase binding

Vocabulary: **INTERPRO**
Term	Definition
IPR011991	ArsR-like_HTH
IPR016157	Cullin_CS
IPR019559	Cullin_neddylation_domain

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