DR749500

EST Overview


Name	DR749500
Unique Name	DR749500
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	369

Libraries


Library Name	Type
LIBEST_017640 cDNA-RAPD Chickpea roots challenged by Fusarium oxysporum f. sp. ciceri Race 1	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of DR749500 vs. SwissProt
Match: GSH1_MEDTR (Glutamate--cysteine ligase, chloroplastic OS=Medicago truncatula GN=GSH1 PE=2 SV=1)

HSP 1 Score: 238.039 bits (606), Expect = 1.103e-62
Identity = 109/113 (96.46%), Postives = 112/113 (99.12%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PFTDGKPNGFVSMRSHIWTDTDK+RTGMLPFVFDDSFGFEQYVD+ALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGE+PTLNDWENHLTTIFPEV
Sbjct:  260 ALQPIATALFANSPFTDGKPNGFVSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDFALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGELPTLNDWENHLTTIFPEV 372

[Close]

BLAST of DR749500 vs. SwissProt
Match: GSH1_ARATH (Glutamate--cysteine ligase, chloroplastic OS=Arabidopsis thaliana GN=GSH1 PE=1 SV=2)

HSP 1 Score: 226.868 bits (577), Expect = 2.543e-59
Identity = 102/113 (90.27%), Postives = 108/113 (95.58%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PFT+GKPNGF+SMRSHIWTDTDK+RTGMLPFVFDDSFGFEQYVDYALDVPMYF YRK KYIDCTGMTFR FLAGKLPC+PGE+P+ NDWENHLTTIFPEV
Sbjct:  274 ALQPIATALFANSPFTEGKPNGFLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRKNKYIDCTGMTFRQFLAGKLPCLPGELPSYNDWENHLTTIFPEV 386

[Close]

BLAST of DR749500 vs. SwissProt
Match: GSH1_BRAJU (Glutamate--cysteine ligase, chloroplastic OS=Brassica juncea GN=GSH1 PE=1 SV=1)

HSP 1 Score: 225.328 bits (573), Expect = 7.398e-59
Identity = 101/113 (89.38%), Postives = 107/113 (94.69%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PFT+GKPNGF+SMRSHIWTDTDK+RTGMLPFVFDDSFGFEQYVDYALDVPMYF YR  KY+DCTGMTFR FLAGKLPC+PGE+PT NDWENHLTTIFPEV
Sbjct:  266 ALQPIATALFANSPFTEGKPNGFLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRNGKYVDCTGMTFRQFLAGKLPCLPGELPTYNDWENHLTTIFPEV 378

[Close]

BLAST of DR749500 vs. SwissProt
Match: GSH1_SOLLC (Glutamate--cysteine ligase, chloroplastic OS=Solanum lycopersicum GN=GSH1 PE=2 SV=1)

HSP 1 Score: 219.55 bits (558), Expect = 4.060e-57
Identity = 99/113 (87.61%), Postives = 106/113 (93.81%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PFT+GKPNG++S RSHIWTDTD NR GMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKY+DCTG++FRDF+ GKLP IPGE PTLNDWENHLTTIFPEV
Sbjct:  275 ALQPIATALFANSPFTEGKPNGYLSKRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYVDCTGLSFRDFMNGKLPPIPGEYPTLNDWENHLTTIFPEV 387

[Close]

BLAST of DR749500 vs. SwissProt
Match: GSH1_TOBAC (Glutamate--cysteine ligase, chloroplastic OS=Nicotiana tabacum GN=GSH1 PE=2 SV=2)

HSP 1 Score: 217.238 bits (552), Expect = 2.015e-56
Identity = 99/113 (87.61%), Postives = 105/113 (92.92%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PFT+GKPNG++SMRSHIWTDTD NR GMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDC GM+FRDF+ GKL  IPG+ PTLNDWENHLTTIFPEV
Sbjct:  274 ALQPIATALFANSPFTEGKPNGYLSMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDFMNGKLSPIPGDYPTLNDWENHLTTIFPEV 386

[Close]

BLAST of DR749500 vs. SwissProt
Match: GSH1B_ORYSJ (Glutamate--cysteine ligase B, chloroplastic OS=Oryza sativa subsp. japonica GN=GSH1-2 PE=3 SV=1)

HSP 1 Score: 216.083 bits (549), Expect = 4.488e-56
Identity = 96/113 (84.96%), Postives = 106/113 (93.81%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATA+FAN PF +GKPNG++S+RSHIWTDTD NR+GMLPFVFDDSFGFE+YVDYALDVPMYFVYR KKYIDCTGM+FRDF+ GKLP  PGE+PTLNDWENHLTTIFPEV
Sbjct:  248 ALQPIATAIFANSPFKEGKPNGYLSLRSHIWTDTDNNRSGMLPFVFDDSFGFERYVDYALDVPMYFVYRNKKYIDCTGMSFRDFMVGKLPQAPGELPTLNDWENHLTTIFPEV 360

[Close]

BLAST of DR749500 vs. SwissProt
Match: GSH1B_ORYSI (Glutamate--cysteine ligase B, chloroplastic OS=Oryza sativa subsp. indica GN=GSH1-2 PE=3 SV=2)

HSP 1 Score: 216.083 bits (549), Expect = 4.488e-56
Identity = 96/113 (84.96%), Postives = 106/113 (93.81%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATA+FAN PF +GKPNG++S+RSHIWTDTD NR+GMLPFVFDDSFGFE+YVDYALDVPMYFVYR KKYIDCTGM+FRDF+ GKLP  PGE+PTLNDWENHLTTIFPEV
Sbjct:  248 ALQPIATAIFANSPFKEGKPNGYLSLRSHIWTDTDNNRSGMLPFVFDDSFGFERYVDYALDVPMYFVYRNKKYIDCTGMSFRDFMVGKLPQAPGELPTLNDWENHLTTIFPEV 360

[Close]

BLAST of DR749500 vs. SwissProt
Match: GSH1A_ORYSI (Glutamate--cysteine ligase A, chloroplastic OS=Oryza sativa subsp. indica GN=GSH1-1 PE=2 SV=2)

HSP 1 Score: 216.083 bits (549), Expect = 4.488e-56
Identity = 96/113 (84.96%), Postives = 106/113 (93.81%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATA+FAN PF +GKPNG++S+RSHIWTDTD NR+GMLPFVFDDSFGFE+YVDYALDVPMYFVYR KKYIDCTGM+FRDF+ GKLP  PGE+PTLNDWENHLTTIFPEV
Sbjct:  244 ALQPIATAIFANSPFKEGKPNGYLSLRSHIWTDTDNNRSGMLPFVFDDSFGFERYVDYALDVPMYFVYRNKKYIDCTGMSFRDFMVGKLPQAPGELPTLNDWENHLTTIFPEV 356

[Close]

BLAST of DR749500 vs. SwissProt
Match: GSH1A_ORYSJ (Glutamate--cysteine ligase A, chloroplastic OS=Oryza sativa subsp. japonica GN=GSH1-1 PE=2 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 5.862e-56
Identity = 95/113 (84.07%), Postives = 106/113 (93.81%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATA+FAN PF +GKPNG++S+RSHIWTDTD NR+GMLPFVFDDSFGFE+YVDYALD+PMYFVYR KKYIDCTGM+FRDF+ GKLP  PGE+PTLNDWENHLTTIFPEV
Sbjct:  244 ALQPIATAIFANSPFKEGKPNGYLSLRSHIWTDTDNNRSGMLPFVFDDSFGFERYVDYALDIPMYFVYRNKKYIDCTGMSFRDFMVGKLPQAPGELPTLNDWENHLTTIFPEV 356

[Close]

BLAST of DR749500 vs. TrEMBL
Match: Q9SEH0_PEA (Gamma-glutamylcysteine synthetase OS=Pisum sativum GN=gsh1 PE=2 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 8.575e-61
Identity = 107/113 (94.69%), Postives = 111/113 (98.23%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PFT+GKPNGFVSMRSHIWTDTDK+RTGMLPFVFDDSFGFEQYVDYALDVPMYF YRKKKY+DCTGMTFRDFLAGKLPCIPGE+PTLNDWENHLTTIFPEV
Sbjct:  251 ALQPIATALFANSPFTEGKPNGFVSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRKKKYVDCTGMTFRDFLAGKLPCIPGELPTLNDWENHLTTIFPEV 363

[Close]

BLAST of DR749500 vs. TrEMBL
Match: A6XHG9_FAGSY (Putative gamma-glutamylcysteine synthetase (Fragment) OS=Fagus sylvatica GN=ECS PE=2 SV=1)

HSP 1 Score: 233.802 bits (595), Expect = 4.256e-60
Identity = 106/113 (93.81%), Postives = 111/113 (98.23%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PFT+GKPNG++S RSHIWTDTDKNR+GMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGE+PTLNDWENHLTTIFPEV
Sbjct:   57 ALQPIATALFANSPFTEGKPNGYLSKRSHIWTDTDKNRSGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGELPTLNDWENHLTTIFPEV 169

[Close]

BLAST of DR749500 vs. TrEMBL
Match: B9RCB6_RICCO (Glutamate--cysteine ligase, chloroplast, putative OS=Ricinus communis GN=RCOM_1686670 PE=4 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 9.481e-60
Identity = 106/113 (93.81%), Postives = 111/113 (98.23%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PFT+GKPNG++SMRS IWTDTDK+RTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGE+PTLNDWENHLTTIFPEV
Sbjct:  278 ALQPIATALFANSPFTEGKPNGYLSMRSQIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGELPTLNDWENHLTTIFPEV 390

[Close]

BLAST of DR749500 vs. TrEMBL
Match: Q6XXZ2_LOTJA (Gamma-glutamylcysteine synthetase OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 230.72 bits (587), Expect = 3.603e-59
Identity = 105/113 (92.92%), Postives = 110/113 (97.35%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PF +GKPNG+VSMRSHIWTDTDK+R+GMLPFVFDDSFGFEQYVDYALDVPMYFVYRK KYIDCTGMTFRDFLAGKLPCIPGE+PTLNDWENHLTTIFPEV
Sbjct:  247 ALQPIATALFANSPFKEGKPNGYVSMRSHIWTDTDKDRSGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKHKYIDCTGMTFRDFLAGKLPCIPGELPTLNDWENHLTTIFPEV 359

[Close]

BLAST of DR749500 vs. TrEMBL
Match: Q9SEH1_PHAVU (Gamma-glutamylcysteine synthetase OS=Phaseolus vulgaris GN=gsh1 PE=2 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 1.369e-58
Identity = 104/113 (92.04%), Postives = 109/113 (96.46%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PF +GKPNGFVSMRSHIWTDTDK+RTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRK +YIDCTG TFRDFLAG+LPCIPGE+PTLNDWENHLTTIFPEV
Sbjct:  260 ALQPIATALFANSPFKEGKPNGFVSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKHRYIDCTGKTFRDFLAGRLPCIPGELPTLNDWENHLTTIFPEV 372

[Close]

BLAST of DR749500 vs. TrEMBL
Match: Q7Y0R3_BRAJU (Glutamate-cysteine ligase OS=Brassica juncea GN=gsh1 PE=2 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 2.335e-58
Identity = 102/113 (90.27%), Postives = 108/113 (95.58%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PFT+GKPNGF+SMRSHIWTDTDK+RTGMLPFVFDDSFGFEQYVDYALDVPMYF YR KKY+DCTGMTFR FLAGKLPC+PGE+PT NDWENHLTTIFPEV
Sbjct:  267 ALQPIATALFANSPFTEGKPNGFLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRNKKYVDCTGMTFRQFLAGKLPCLPGELPTYNDWENHLTTIFPEV 379

[Close]

BLAST of DR749500 vs. TrEMBL
Match: B9GKI0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_709341 PE=4 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 2.335e-58
Identity = 102/113 (90.27%), Postives = 110/113 (97.35%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PFT+GKPNG++S+RSHIWTDTD NR GMLPFVF+D+FGFEQYVDYALDVPMYFVYRKKKYIDCTGM+FRDFLAGKLPCIPGE+PTLNDWENHLTTIFPEV
Sbjct:  278 ALQPIATALFANSPFTEGKPNGYLSLRSHIWTDTDNNRAGMLPFVFNDTFGFEQYVDYALDVPMYFVYRKKKYIDCTGMSFRDFLAGKLPCIPGELPTLNDWENHLTTIFPEV 390

[Close]

BLAST of DR749500 vs. TrEMBL
Match: B6EU03_BRANA (Putative glutamyl cysteine ligase OS=Brassica napus GN=ecs2 PE=4 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 2.335e-58
Identity = 102/113 (90.27%), Postives = 108/113 (95.58%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PFT+GKPNGF+SMRSHIWTDTDK+RTGMLPFVFDDSFGFEQYVDYALDVPMYF YR KKY+DCTGMTFR FLAGKLPC+PGE+PT NDWENHLTTIFPEV
Sbjct:  255 ALQPIATALFANSPFTEGKPNGFLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRNKKYVDCTGMTFRQFLAGKLPCLPGELPTYNDWENHLTTIFPEV 367

[Close]

BLAST of DR749500 vs. TrEMBL
Match: B9GWI2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_830837 PE=4 SV=1)

HSP 1 Score: 227.639 bits (579), Expect = 3.050e-58
Identity = 102/113 (90.27%), Postives = 109/113 (96.46%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PFTDGKPNG++S RSHIWTDTD NR GMLPFVFDD+FGFEQYVDYALDVPMYFVYRK+KYIDCTGM+FRDFLAG+LPCIPGE+PTLNDWENHLTTIFPEV
Sbjct:  278 ALQPIATALFANSPFTDGKPNGYLSKRSHIWTDTDNNRAGMLPFVFDDNFGFEQYVDYALDVPMYFVYRKEKYIDCTGMSFRDFLAGRLPCIPGELPTLNDWENHLTTIFPEV 390

[Close]

BLAST of DR749500 vs. TrEMBL
Match: Q2V3F8_ARATH (Putative uncharacterized protein At4g23100.2 OS=Arabidopsis thaliana GN=At4g23100 PE=4 SV=1)

HSP 1 Score: 226.868 bits (577), Expect = 5.202e-58
Identity = 102/113 (90.27%), Postives = 108/113 (95.58%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PFT+GKPNGF+SMRSHIWTDTDK+RTGMLPFVFDDSFGFEQYVDYALDVPMYF YRK KYIDCTGMTFR FLAGKLPC+PGE+P+ NDWENHLTTIFPEV
Sbjct:  274 ALQPIATALFANSPFTEGKPNGFLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRKNKYIDCTGMTFRQFLAGKLPCLPGELPSYNDWENHLTTIFPEV 386

[Close]

BLAST of DR749500 vs. TAIR peptide
Match: AT4G23100.3 (| Symbols: GSH1 | glutamate-cysteine ligase | chr4:12103458-12106751 REVERSE LENGTH=522)

HSP 1 Score: 226.868 bits (577), Expect = 2.246e-60
Identity = 102/113 (90.27%), Postives = 108/113 (95.58%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PFT+GKPNGF+SMRSHIWTDTDK+RTGMLPFVFDDSFGFEQYVDYALDVPMYF YRK KYIDCTGMTFR FLAGKLPC+PGE+P+ NDWENHLTTIFPEV
Sbjct:  274 ALQPIATALFANSPFTEGKPNGFLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRKNKYIDCTGMTFRQFLAGKLPCLPGELPSYNDWENHLTTIFPEV 386

[Close]

BLAST of DR749500 vs. TAIR peptide
Match: AT4G23100.2 (| Symbols: RML1, PAD2, GSH1, CAD2 | glutamate-cysteine ligase | chr4:12103779-12106751 REVERSE LENGTH=477)

HSP 1 Score: 226.868 bits (577), Expect = 2.246e-60
Identity = 102/113 (90.27%), Postives = 108/113 (95.58%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PFT+GKPNGF+SMRSHIWTDTDK+RTGMLPFVFDDSFGFEQYVDYALDVPMYF YRK KYIDCTGMTFR FLAGKLPC+PGE+P+ NDWENHLTTIFPEV
Sbjct:  274 ALQPIATALFANSPFTEGKPNGFLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRKNKYIDCTGMTFRQFLAGKLPCLPGELPSYNDWENHLTTIFPEV 386

[Close]

BLAST of DR749500 vs. TAIR peptide
Match: AT4G23100.1 (| Symbols: RML1, PAD2, GSH1, CAD2, ATECS1, GSHA | glutamate-cysteine ligase | chr4:12103458-12106751 REVERSE LENGTH=522)

HSP 1 Score: 226.868 bits (577), Expect = 2.246e-60
Identity = 102/113 (90.27%), Postives = 108/113 (95.58%), Query Frame = -1

Query:   31 ALQPIATALFANPPFTDGKPNGFVSMRSHIWTDTDKNRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGEIPTLNDWENHLTTIFPEV 369
            ALQPIATALFAN PFT+GKPNGF+SMRSHIWTDTDK+RTGMLPFVFDDSFGFEQYVDYALDVPMYF YRK KYIDCTGMTFR FLAGKLPC+PGE+P+ NDWENHLTTIFPEV
Sbjct:  274 ALQPIATALFANSPFTEGKPNGFLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRKNKYIDCTGMTFRQFLAGKLPCLPGELPSYNDWENHLTTIFPEV 386

The following BLAST results are available for this feature:

BLAST of DR749500 vs. SwissProt
BLAST of DR749500 vs. TrEMBL
BLAST of DR749500 vs. TAIR peptide

BLAST of DR749500 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 9


Match Name	E-value	Identity	Description
GSH1_MEDTR	1.103e-62	96.46	Glutamate--cysteine ligase, chloroplastic OS=Medic...	[more]
GSH1_ARATH	2.543e-59	90.27	Glutamate--cysteine ligase, chloroplastic OS=Arabi...	[more]
GSH1_BRAJU	7.398e-59	89.38	Glutamate--cysteine ligase, chloroplastic OS=Brass...	[more]
GSH1_SOLLC	4.060e-57	87.61	Glutamate--cysteine ligase, chloroplastic OS=Solan...	[more]
GSH1_TOBAC	2.015e-56	87.61	Glutamate--cysteine ligase, chloroplastic OS=Nicot...	[more]
GSH1B_ORYSJ	4.488e-56	84.96	Glutamate--cysteine ligase B, chloroplastic OS=Ory...	[more]
GSH1B_ORYSI	4.488e-56	84.96	Glutamate--cysteine ligase B, chloroplastic OS=Ory...	[more]
GSH1A_ORYSI	4.488e-56	84.96	Glutamate--cysteine ligase A, chloroplastic OS=Ory...	[more]
GSH1A_ORYSJ	5.862e-56	84.07	Glutamate--cysteine ligase A, chloroplastic OS=Ory...	[more]

BLAST of DR749500 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9SEH0_PEA	8.575e-61	94.69	Gamma-glutamylcysteine synthetase OS=Pisum sativum...	[more]
A6XHG9_FAGSY	4.256e-60	93.81	Putative gamma-glutamylcysteine synthetase (Fragme...	[more]
B9RCB6_RICCO	9.481e-60	93.81	Glutamate--cysteine ligase, chloroplast, putative ...	[more]
Q6XXZ2_LOTJA	3.603e-59	92.92	Gamma-glutamylcysteine synthetase OS=Lotus japonic...	[more]
Q9SEH1_PHAVU	1.369e-58	92.04	Gamma-glutamylcysteine synthetase OS=Phaseolus vul...	[more]
Q7Y0R3_BRAJU	2.335e-58	90.27	Glutamate-cysteine ligase OS=Brassica juncea GN=gs...	[more]
B9GKI0_POPTR	2.335e-58	90.27	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B6EU03_BRANA	2.335e-58	90.27	Putative glutamyl cysteine ligase OS=Brassica napu...	[more]
B9GWI2_POPTR	3.050e-58	90.27	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q2V3F8_ARATH	5.202e-58	90.27	Putative uncharacterized protein At4g23100.2 OS=Ar...	[more]

BLAST of DR749500 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G23100.3	2.246e-60	90.27	\| Symbols: GSH1 \| glutamate-cysteine ligase \| chr4...	[more]
AT4G23100.2	2.246e-60	90.27	\| Symbols: RML1, PAD2, GSH1, CAD2 \| glutamate-cyst...	[more]
AT4G23100.1	2.246e-60	90.27	\| Symbols: RML1, PAD2, GSH1, CAD2, ATECS1, GSHA \| ...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006336	Glutamate--cysteine ligase, GCS2	PFAM	PF04107	GCS2	coord: 1..116 score: 3.3
None	No IPR available	SUPERFAMILY	55931	Glutamine synthetase/guanido kinase	coord: 1..113 score: 4.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>DR749500 ID=DR749500; Name=DR749500; organism=Cicer arietinum; type=EST; length=369bp
CGCCGCATGTTTGCGCGGGAATCGATTTGTGACCTCAGGAAATATAGTGG
TCAAATGATTCTCCCAATCATTGAGAGTTGGTATTTCACCAGGAATACAA
GGAAGCTTTCCTGCCAAGAAGTCCCTGAAGGTCATTCCAGTACAGTCGAT
GTACTTCTTTTTCCGATAGACAAAATACATTGGAACATCAAGAGCATAAT
CAACGTACTGCTCAAACCCGAAGGAGTCATCAAAAACAAAAGGCAGCATG
CCTGTGCGGTTCTTATCAGTGTCAGTCCAAATATGACTTCTCATACTGAC
AAATCCATTGGGCTTTCCATCTGTGAAGGGTGGATTTGCAAAAAGAGCTG
TTGCAATAGGTTGCAAAGC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004357	glutamate-cysteine ligase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006750	glutathione biosynthetic process

Vocabulary: **INTERPRO**
Term	Definition
IPR006336	GCS2

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