HO063057

EST Overview


Name	HO063057
Unique Name	HO063057
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	680

Libraries


Library Name	Type
LIBEST_026409 Chickpea drought stressed cDNA SSH library AB2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO063057 vs. SwissProt
Match: CHS3_CAMSI (Chalcone synthase 3 OS=Camellia sinensis GN=CHS3 PE=2 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 2.206e-48
Identity = 72/83 (86.75%), Postives = 78/83 (93.98%), Query Frame = -3

Query:   94 GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSV 342
            GGPAILDQVE KL LK EK+RATR VLSEYGNMSS CVLFILDEMR+KSA++GLKTTGEGLEWGVLFGFGPGLT+ETVVLHS+
Sbjct:  305 GGPAILDQVELKLGLKEEKLRATRHVLSEYGNMSSACVLFILDEMRKKSAEEGLKTTGEGLEWGVLFGFGPGLTVETVVLHSL 387

HSP 2 Score: 62.3882 bits (150), Expect = 2.206e-48
Identity = 28/48 (58.33%), Postives = 37/48 (77.08%), Query Frame = -2

Query:  341 LGKAGLTFPLFKDVPGIVSKNIDKALIEAFHPLNIFVYNSIFGIAHPG 484
            L + GLTF L KDVP ++S N++K+L+EAF PL I  +NS+F IAHPG
Sbjct:  258 LREVGLTFHLLKDVPRLISMNVEKSLVEAFQPLGISDWNSLFWIAHPG 305

HSP 3 Score: 23.0978 bits (48), Expect = 2.206e-48
Identity = 15/38 (39.47%), Postives = 18/38 (47.37%), Query Frame = -1

Query:  555 PRETHLDSLVEQXXXXXXXXXLNGGF*SQFPEIEKTKF 668
            P +THLDSLV Q         +  G     PE+EK  F
Sbjct:  201 PSDTHLDSLVGQSLFGDGAAAIIIGS-DPIPEVEKPLF 237

[Close]

BLAST of HO063057 vs. SwissProt
Match: CHS1_CICAR (Chalcone synthase 1 OS=Cicer arietinum GN=CHS1 PE=2 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 1.043e-42
Identity = 106/166 (63.86%), Postives = 116/166 (69.88%), Query Frame = -3

Query:   88 PIPGN*ENQIFEDGLGRHKPIAPR*GKGAIDGHLWGKQDSHFPFLKMFRGLSQRTLIKL*LKLSTH*TYLFTIQYLGLHIQGGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 585
            PIP   E  IFE  +   + IAP   +GAIDGHL  +    F  LK   G+  + + K  ++               +   GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSS CVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI
Sbjct:  228 PIP-EIEKPIFEM-VWTAQTIAPD-SEGAIDGHL-REAGLTFHLLKDVPGIVSKNIDKALIEAFQPLNISDYNSIFWIAHPGGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSACVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 389

[Close]

BLAST of HO063057 vs. SwissProt
Match: CHSF_PETHY (Chalcone synthase F OS=Petunia hybrida GN=CHSF PE=2 SV=2)

HSP 1 Score: 126.716 bits (317), Expect = 1.667e-42
Identity = 61/85 (71.76%), Postives = 69/85 (81.18%), Query Frame = -3

Query:   88 GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 342
            GG  ILDQVE KL LKPEK+  TR V+SEYGNM+S CVLF+LDEMR+ S K+G  T  EGLEWGVL  FGPGLTIET+VLHSV+I
Sbjct:  305 GGREILDQVELKLGLKPEKLNVTRHVMSEYGNMASACVLFVLDEMRKTSTKEGFGTNVEGLEWGVLCSFGPGLTIETIVLHSVSI 389

HSP 2 Score: 67.3958 bits (163), Expect = 1.667e-42
Identity = 39/87 (44.83%), Postives = 51/87 (58.62%), Query Frame = -2

Query:  341 MVGFDPNSRKLRKPNF*RWFGXAQTNCSQIGERCY*WSPLGKAGLTFPLFKDVPGIVSKNIDKALIEAFHPLNIFVYNSIFGIAHPG 601
            ++G DPN  ++ KP F      AQT     G + Y        GLTF L KDVP +VS+NI+K+L+E F PL IF +NSIF + H G
Sbjct:  223 IIGSDPNL-EVEKPLF-ELVSAAQTLVPDCGHKIY--GKTSDVGLTFHLHKDVPRLVSQNIEKSLVEVFQPLGIFDWNSIFWVGHAG 305

[Close]

BLAST of HO063057 vs. SwissProt
Match: CHSG_PETHY (Chalcone synthase G OS=Petunia hybrida GN=CHSG PE=2 SV=2)

HSP 1 Score: 139.428 bits (350), Expect = 3.687e-42
Identity = 66/85 (77.65%), Postives = 76/85 (89.41%), Query Frame = -3

Query:   88 GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 342
            GGPAIL+Q+E KL LKPEK+RA R VLSEYGNMSS CVLF+LDEMR+ + + G+ TTGEGLEWG+LFGFGPGLTIETVVLHSV+I
Sbjct:  308 GGPAILNQIELKLGLKPEKLRAARHVLSEYGNMSSACVLFVLDEMRKGTIEKGMGTTGEGLEWGLLFGFGPGLTIETVVLHSVSI 392

HSP 2 Score: 53.5286 bits (127), Expect = 3.687e-42
Identity = 25/48 (52.08%), Postives = 34/48 (70.83%), Query Frame = -2

Query:  341 LGKAGLTFPLFKDVPGIVSKNIDKALIEAFHPLNIFVYNSIFGIAHPG 484
            L +AGL   L KD P ++S+NI++ L+E F PL I  +NSIF +AHPG
Sbjct:  261 LTEAGLIPHLLKDAPIVISQNIERRLVEVFKPLGISDWNSIFWVAHPG 308

[Close]

BLAST of HO063057 vs. SwissProt
Match: CHS3_TRISU (Chalcone synthase 3 OS=Trifolium subterraneum GN=CHS3 PE=2 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 8.158e-42
Identity = 94/146 (64.38%), Postives = 106/146 (72.60%), Query Frame = -3

Query:   88 IAPR*GKGAIDGHLWGKQDSHFPFLKMFRGLSQRTLIKL*LKLSTH*TYLFTIQYLGLHIQGGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 525
            IAP   +GAIDGHL  +    F  LK   G+  + + K  ++               +   GGPAILDQVE+KLALKPEKM+ATREVLSEYGNMSS CVLFILDEMR+KS KDGLKTTGEGL+WGVLFGFGPGLTIETVVLHSVAI
Sbjct:  246 IAPD-SEGAIDGHL-REAGLTFHLLKDVPGIVSKNIDKALVEAFQPLGISDYNSIFWIAHPGGPAILDQVEQKLALKPEKMKATREVLSEYGNMSSACVLFILDEMRKKSTKDGLKTTGEGLDWGVLFGFGPGLTIETVVLHSVAI 389

HSP 2 Score: 26.5646 bits (57), Expect = 8.158e-42
Identity = 20/43 (46.51%), Postives = 22/43 (51.16%), Query Frame = -1

Query:  540 PRETHLDSLVEQXXXXXXXXXLNGGF*SQFPEIEKTKFLKMVW 668
            P +THLDSLV Q         L  G     PEIEK  F+ MVW
Sbjct:  201 PSDTHLDSLVGQALFGDGAAALIVGS-DPVPEIEKPIFV-MVW 241

[Close]

BLAST of HO063057 vs. SwissProt
Match: CHSY_EQUAR (Chalcone synthase OS=Equisetum arvense GN=CHS PE=2 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 1.193e-41
Identity = 65/85 (76.47%), Postives = 76/85 (89.41%), Query Frame = -3

Query:   88 GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 342
            GGPAILDQVEEK+ L PEKMRATR+VLSEYGNMSS CVLFI+D MRR SA++ L+TTGEGL+WGVL GFGPGLT+ETV+L S+ +
Sbjct:  319 GGPAILDQVEEKMKLAPEKMRATRDVLSEYGNMSSACVLFIMDHMRRMSAQNKLQTTGEGLDWGVLLGFGPGLTVETVLLKSIRL 403

HSP 2 Score: 48.1358 bits (113), Expect = 1.193e-41
Identity = 29/60 (48.33%), Postives = 34/60 (56.67%), Query Frame = -2

Query:  341 LGKAGLTFPLFKDVPGIVSKNIDKALIEAF------------HPLNIFVYNSIFGIAHPG 484
            L + GLTF L KDVPGI+SKNI K L +AF             P ++   N IF IAHPG
Sbjct:  263 LREVGLTFHLMKDVPGIISKNIGKVLNDAFRSAFDESGNAEDRPASV---NDIFWIAHPG 319

HSP 3 Score: 25.7942 bits (55), Expect = 1.193e-41
Identity = 19/52 (36.54%), Postives = 25/52 (48.08%), Query Frame = -1

Query:  513 PRETHLDSLVEQXXXXXXXXXLNGGF*SQFPEIEKTKFLKMVWXGTNQLLPD 668
            P ETHLDSLV Q         +  G     P  E+    ++ W G+N +LPD
Sbjct:  207 PSETHLDSLVGQALFGDGAAAVILG---SDPLPEENPCFELHWSGSN-ILPD 254

[Close]

BLAST of HO063057 vs. SwissProt
Match: CHS6_PEA (Chalcone synthase 6 OS=Pisum sativum GN=CHS6 PE=2 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 3.992e-41
Identity = 93/146 (63.70%), Postives = 105/146 (71.92%), Query Frame = -3

Query:   88 IAPR*GKGAIDGHLWGKQDSHFPFLKMFRGLSQRTLIKL*LKLSTH*TYLFTIQYLGLHIQGGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 525
            IAP   +GAIDGHL  +    F  LK   G+  + + K  ++               +   GGPAILDQVEEKL LKPEKM+ATREVLSEYGNMSS CVLFILDEMR+KSA+ GLKTTGEGL+WGVLFGFGPGLTIETVVLHSVAI
Sbjct:  246 IAPD-SEGAIDGHL-REAGLTFHLLKDVPGIVSKNINKALVEAFQPLNIDDYNSIFWIAHPGGPAILDQVEEKLGLKPEKMKATREVLSEYGNMSSACVLFILDEMRKKSAQQGLKTTGEGLDWGVLFGFGPGLTIETVVLHSVAI 389

HSP 2 Score: 26.9498 bits (58), Expect = 3.992e-41
Identity = 20/43 (46.51%), Postives = 22/43 (51.16%), Query Frame = -1

Query:  540 PRETHLDSLVEQXXXXXXXXXLNGGF*SQFPEIEKTKFLKMVW 668
            P +THLDSLV Q         L  G     PEIEK  F +MVW
Sbjct:  201 PSDTHLDSLVGQALFGDGAAALIVGS-DPLPEIEKPIF-EMVW 241

[Close]

BLAST of HO063057 vs. SwissProt
Match: CHSD_PETHY (Chalcone synthase D OS=Petunia hybrida GN=CHSD PE=3 SV=2)

HSP 1 Score: 127.872 bits (320), Expect = 1.498e-40
Identity = 60/84 (71.43%), Postives = 70/84 (83.33%), Query Frame = -3

Query:   91 GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVA 342
            GGPAIL+QVE KL L PEK+ ATR VLSEYGNMSS  +LF+LDEMR+ S + G  TTGEGL+WGVL GFGPG+T ET+VLHSV+
Sbjct:  305 GGPAILNQVELKLGLNPEKLGATRHVLSEYGNMSSASILFVLDEMRKSSTQKGFDTTGEGLKWGVLIGFGPGITFETIVLHSVS 388

HSP 2 Score: 59.6918 bits (143), Expect = 1.498e-40
Identity = 27/48 (56.25%), Postives = 35/48 (72.92%), Query Frame = -2

Query:  341 LGKAGLTFPLFKDVPGIVSKNIDKALIEAFHPLNIFVYNSIFGIAHPG 484
            L + GLTF L KDV  ++S NI+K+L+E F PL I  +NSIF +AHPG
Sbjct:  258 LREIGLTFHLLKDVAELISNNIEKSLVEVFQPLGISAWNSIFWVAHPG 305

[Close]

BLAST of HO063057 vs. SwissProt
Match: CHS2_TRISU (Chalcone synthase 2 OS=Trifolium subterraneum GN=CHS2 PE=3 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 2.174e-40
Identity = 100/166 (60.24%), Postives = 116/166 (69.88%), Query Frame = -3

Query:   88 PIPGN*ENQIFEDGLGRHKPIAPR*GKGAIDGHLWGKQDSHFPFLKMFRGLSQRTLIKL*LKLSTH*TYLFTIQYLGLHIQGGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 585
            P+P   E  IFE  +   + IAP   +GAIDGHL  +    F  LK   G+  + + K  ++               +   GGPAILDQVE+KLALKPEKM+ATREVLSEYGNMSS CVLFILDEMR+KSA++GLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI
Sbjct:  228 PVP-EIEKPIFEM-VWTAQTIAPD-SEGAIDGHL-REAGLTFHLLKDVPGIVSKNIDKALVEAFQPLNISDYNSIFWIAHPGGPAILDQVEQKLALKPEKMKATREVLSEYGNMSSACVLFILDEMRKKSAQNGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 389

[Close]

BLAST of HO063057 vs. SwissProt
Match: CHS4_PEA (Chalcone synthase 4 OS=Pisum sativum GN=CHS4 PE=3 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 2.545e-40
Identity = 92/146 (63.01%), Postives = 104/146 (71.23%), Query Frame = -3

Query:   88 IAPR*GKGAIDGHLWGKQDSHFPFLKMFRGLSQRTLIKL*LKLSTH*TYLFTIQYLGLHIQGGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 525
            IAP   +GAIDGHL  +    F  LK    +  + + K  ++               +   GGPAILDQVE+KLALKPEKM+ATREVLSEYGNMSS CVLFILDEMRRKS ++GLKTTGEGLEWGVLFGFGPGLTIETVVLHSV I
Sbjct:  246 IAPD-SEGAIDGHL-REAGLTFHLLKDVPAIVSKNIDKALVEAFQPLGISDYNSIFWIAHPGGPAILDQVEQKLALKPEKMKATREVLSEYGNMSSACVLFILDEMRRKSIQNGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVVI 389

HSP 2 Score: 26.9498 bits (58), Expect = 2.545e-40
Identity = 20/43 (46.51%), Postives = 22/43 (51.16%), Query Frame = -1

Query:  540 PRETHLDSLVEQXXXXXXXXXLNGGF*SQFPEIEKTKFLKMVW 668
            P +THLDSLV Q         L  G     PEIEK  F +MVW
Sbjct:  201 PSDTHLDSLVGQALFGDGAAALIVGS-DPLPEIEKPIF-EMVW 241

[Close]

BLAST of HO063057 vs. TrEMBL
Match: Q7Y1X9_LUPLU (Chalcone synthase OS=Lupinus luteus GN=CHS PE=2 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 5.531e-51
Identity = 76/83 (91.57%), Postives = 77/83 (92.77%), Query Frame = -3

Query:   91 GPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVA 339
            GPAILDQVE KLALKPEKMRATR VLSEYGNMSS CVL ILDEMRRKS +DGLKTTGEGLEWGV FGFGPGLTIETVVLHSVA
Sbjct:  305 GPAILDQVEAKLALKPEKMRATRHVLSEYGNMSSACVLIILDEMRRKSKEDGLKTTGEGLEWGVFFGFGPGLTIETVVLHSVA 387

HSP 2 Score: 68.5514 bits (166), Expect = 5.531e-51
Identity = 36/47 (76.60%), Postives = 39/47 (82.98%), Query Frame = -2

Query:  344 LGKAGLTFPLFKDVPGIVSKNIDKALIEAFHPLNIFVYNSIFGIAHP 484
            L + GLTF L  DVPGIVSKNIDKAL+EAF+PLNI  YNSIF IAHP
Sbjct:  258 LREVGLTFHLL-DVPGIVSKNIDKALVEAFNPLNISDYNSIFWIAHP 303

HSP 3 Score: 25.409 bits (54), Expect = 5.531e-51
Identity = 19/43 (44.19%), Postives = 22/43 (51.16%), Query Frame = -1

Query:  540 PRETHLDSLVEQXXXXXXXXXLNGGF*SQFPEIEKTKFLKMVW 668
            P +THLDSLV Q         L  G     PEIEK  F ++VW
Sbjct:  201 PSDTHLDSLVGQALFGDGAAALIVGS-DPVPEIEKPIF-ELVW 241

[Close]

BLAST of HO063057 vs. TrEMBL
Match: Q460Q1_ARAGE (Chalcone synthase family protein OS=Arabis gemmifera GN=CHS5 PE=2 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 2.353e-48
Identity = 75/85 (88.24%), Postives = 78/85 (91.76%), Query Frame = -3

Query:   88 GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 342
            GGPAILDQVE KL LK EKMRATR VLSEYGNMSS CVLFILDEMRRKSAKDG+ TTGEGLEWGVLFGFGPGLT+ETVVLHSV +
Sbjct:  312 GGPAILDQVEIKLGLKEEKMRATRHVLSEYGNMSSACVLFILDEMRRKSAKDGVATTGEGLEWGVLFGFGPGLTVETVVLHSVPL 396

HSP 2 Score: 63.5438 bits (153), Expect = 2.353e-48
Identity = 41/87 (47.13%), Postives = 50/87 (57.47%), Query Frame = -2

Query:  341 MVGFDPNSRKLRKPNF*RWFGXAQTNCSQIGERCY*WSPLGKAGLTFPLFKDVPGIVSKNIDKALIEAFHPLNIFVYNSIFGIAHPG 601
            +VG DP++    KP F      AQT              L + GLTF LFKDVPG++SKNI K+L EAF P+ I  +NS F IAHPG
Sbjct:  229 IVGSDPDTSVGEKPIF-EMVSAAQTILPDSDGAID--GHLREVGLTFHLFKDVPGLISKNIVKSLDEAFKPMGISDWNSPFWIAHPG 312

HSP 3 Score: 22.3274 bits (46), Expect = 2.353e-48
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = -1

Query:  633 PRETHLDSLVEQ 668
            P +THLDSLV Q
Sbjct:  207 PSDTHLDSLVGQ 218

[Close]

BLAST of HO063057 vs. TrEMBL
Match: Q690N1_CAMSI (Chalcone synthase OS=Camellia sinensis GN=CHS PE=2 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 3.269e-47
Identity = 72/83 (86.75%), Postives = 78/83 (93.98%), Query Frame = -3

Query:   94 GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSV 342
            GGPAILDQVE KL LK EK+RATR VLSEYGNMSS CVLFILDEMR+KSA++GLKTTGEGLEWGVLFGFGPGLT+ETVVLHS+
Sbjct:  305 GGPAILDQVELKLGLKEEKLRATRHVLSEYGNMSSACVLFILDEMRKKSAEEGLKTTGEGLEWGVLFGFGPGLTVETVVLHSL 387

HSP 2 Score: 62.3882 bits (150), Expect = 3.269e-47
Identity = 28/48 (58.33%), Postives = 37/48 (77.08%), Query Frame = -2

Query:  341 LGKAGLTFPLFKDVPGIVSKNIDKALIEAFHPLNIFVYNSIFGIAHPG 484
            L + GLTF L KDVP ++S N++K+L+EAF PL I  +NS+F IAHPG
Sbjct:  258 LREVGLTFHLLKDVPRLISMNVEKSLVEAFQPLGISDWNSLFWIAHPG 305

HSP 3 Score: 23.0978 bits (48), Expect = 3.269e-47
Identity = 15/38 (39.47%), Postives = 18/38 (47.37%), Query Frame = -1

Query:  555 PRETHLDSLVEQXXXXXXXXXLNGGF*SQFPEIEKTKF 668
            P +THLDSLV Q         +  G     PE+EK  F
Sbjct:  201 PSDTHLDSLVGQSLFGDGAAAIIIGS-DPIPEVEKPLF 237

[Close]

BLAST of HO063057 vs. TrEMBL
Match: Q2WBN0_9LAMI (Chalcone synthase OS=Misopates orontium GN=chs PE=2 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 9.697e-47
Identity = 69/85 (81.18%), Postives = 77/85 (90.59%), Query Frame = -3

Query:   88 GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 342
            GGPAILDQVEEKL LKPEK+R+TR VLSEYGNMSS CVLFILDEMR+ S K+G+ TTGEG +WGVLFGFGPGLT+ETVVLHSV +
Sbjct:  305 GGPAILDQVEEKLGLKPEKLRSTRHVLSEYGNMSSACVLFILDEMRKSSTKEGMSTTGEGFDWGVLFGFGPGLTVETVVLHSVPL 389

HSP 2 Score: 63.929 bits (154), Expect = 9.697e-47
Identity = 30/48 (62.50%), Postives = 38/48 (79.17%), Query Frame = -2

Query:  341 LGKAGLTFPLFKDVPGIVSKNIDKALIEAFHPLNIFVYNSIFGIAHPG 484
            L + GLTF L KDVPG++SK+I+K+L EAF PL I  +NS+F IAHPG
Sbjct:  258 LREVGLTFHLLKDVPGLISKHIEKSLKEAFDPLGISDWNSVFWIAHPG 305

[Close]

BLAST of HO063057 vs. TrEMBL
Match: Q9FS37_9LAMI (Chalcone synthase OS=Torenia hybrid cultivar PE=2 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 1.647e-46
Identity = 70/82 (85.37%), Postives = 77/82 (93.90%), Query Frame = -3

Query:   97 GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHS 342
            GGPAILDQVEEKL LKP+K+RATR VLSEYGNMSS CVLFILDEMR+ SAK+GL +TGEGL+WGVLFGFGPGLT+ETVVLHS
Sbjct:  304 GGPAILDQVEEKLGLKPDKLRATRTVLSEYGNMSSACVLFILDEMRKASAKEGLGSTGEGLDWGVLFGFGPGLTVETVVLHS 385

HSP 2 Score: 64.3142 bits (155), Expect = 1.647e-46
Identity = 32/48 (66.67%), Postives = 37/48 (77.08%), Query Frame = -2

Query:  341 LGKAGLTFPLFKDVPGIVSKNIDKALIEAFHPLNIFVYNSIFGIAHPG 484
            L + GLTF L KDVPGI+SKNI+K+L EAF PL I  +NSIF  AHPG
Sbjct:  257 LREVGLTFHLLKDVPGIISKNIEKSLKEAFDPLGISDWNSIFWNAHPG 304

[Close]

BLAST of HO063057 vs. TrEMBL
Match: Q6X3Y1_IPOTF (Chalcone synthase (Fragment) OS=Ipomoea trifida GN=CHS-D PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 4.560e-46
Identity = 69/78 (88.46%), Postives = 72/78 (92.31%), Query Frame = -3

Query:  109 GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETV 342
            GGPAILDQVEEKL LKPEK+RATR VLSEYGNMSS CVLFILDEMR+ S+ DGL TTGEGLEWGVLFGFGPGLTIETV
Sbjct:  236 GGPAILDQVEEKLGLKPEKLRATRHVLSEYGNMSSACVLFILDEMRKASSNDGLGTTGEGLEWGVLFGFGPGLTIETV 313

HSP 2 Score: 64.6994 bits (156), Expect = 4.560e-46
Identity = 42/91 (46.15%), Postives = 53/91 (58.24%), Query Frame = -2

Query:  341 MVGFDPNSRKLRKPNF*RWFGXAQT----NCSQIGERCY*WSPLGKAGLTFPLFKDVPGIVSKNIDKALIEAFHPLNIFVYNSIFGIAHPG 601
            +VG DP + +  KP F +    AQT    +C  I         L + GLTF L KDVP +VS NI+K L EAF+PL I  +NS+F IAHPG
Sbjct:  155 IVGADPTTAE--KPLF-QLVSAAQTLAPDSCGAIDGH------LREVGLTFHLLKDVPSVVSNNIEKCLFEAFNPLGISDWNSVFWIAHPG 236

HSP 3 Score: 23.483 bits (49), Expect = 4.560e-46
Identity = 10/12 (83.33%), Postives = 10/12 (83.33%), Query Frame = -1

Query:  633 PRETHLDSLVEQ 668
            P ETHLDSLV Q
Sbjct:  133 PSETHLDSLVGQ 144

[Close]

BLAST of HO063057 vs. TrEMBL
Match: Q6PYC2_CAMSI (Chalcone synthase 1 (Fragment) OS=Camellia sinensis PE=2 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 8.281e-46
Identity = 72/84 (85.71%), Postives = 77/84 (91.67%), Query Frame = -3

Query:   91 GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVA 342
            GGPAILDQVE K  LK EK+RATR VLSEYGNMSS CVLFILDEMR+KSA DGLKTTGEGLEWGVLFGFGPGLT+ETVVLHS++
Sbjct:   58 GGPAILDQVELKSGLKEEKLRATRHVLSEYGNMSSACVLFILDEMRKKSAADGLKTTGEGLEWGVLFGFGPGLTVETVVLHSLS 141

HSP 2 Score: 61.2326 bits (147), Expect = 8.281e-46
Identity = 29/48 (60.42%), Postives = 36/48 (75.00%), Query Frame = -2

Query:  341 LGKAGLTFPLFKDVPGIVSKNIDKALIEAFHPLNIFVYNSIFGIAHPG 484
            L +  LTF L KDVPG++SKNI+K+L EAF PL I  +NS+F IA PG
Sbjct:   11 LREVALTFHLLKDVPGLISKNIEKSLAEAFQPLGISDWNSLFWIARPG 58

[Close]

BLAST of HO063057 vs. TrEMBL
Match: Q6PYC1_CAMSI (Chalcone synthase 3 (Fragment) OS=Camellia sinensis PE=2 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 1.970e-44
Identity = 72/83 (86.75%), Postives = 78/83 (93.98%), Query Frame = -3

Query:   94 GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSV 342
            GGPAILDQVE KL LK EK+RATR VLSEYGNMSS CVLFILDEMR+KSA++GLKTTGEGLEWGVLFGFGPGLT+ETVVLHS+
Sbjct:   82 GGPAILDQVELKLGLKEEKLRATRHVLSEYGNMSSACVLFILDEMRKKSAEEGLKTTGEGLEWGVLFGFGPGLTVETVVLHSL 164

HSP 2 Score: 55.4546 bits (132), Expect = 1.970e-44
Identity = 25/48 (52.08%), Postives = 34/48 (70.83%), Query Frame = -2

Query:  341 LGKAGLTFPLFKDVPGIVSKNIDKALIEAFHPLNIFVYNSIFGIAHPG 484
            L +   T  L KDVP ++S N++K+L+EAF PL I  +NS+F IAHPG
Sbjct:   35 LREVACTVHLLKDVPRLISMNVEKSLVEAFQPLGISDWNSLFWIAHPG 82

[Close]

BLAST of HO063057 vs. TrEMBL
Match: B6S394_ROBPS (Chalcone synthase (Fragment) OS=Robinia pseudoacacia PE=2 SV=2)

HSP 1 Score: 167.162 bits (422), Expect = 3.225e-41
Identity = 93/139 (66.91%), Postives = 103/139 (74.10%), Query Frame = -3

Query:   88 GAIDGHLWGKQDSHFPFLKMFRGLSQRTLIKL*LKLSTH*TYLFTIQYLGLHIQGGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 504
            GAIDGHL  +    F  LK   G+  + + K  ++               +   GGPAILDQVE+KL+LKPEKMRATREVLSEYGNMSS CVLFILDEMR+KSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI
Sbjct:  165 GAIDGHL-REVGLTFHLLKDVPGIVSKNVDKALVEAFQPLGISDYNSIFWIAXPGGPAILDQVEQKLSLKPEKMRATREVLSEYGNMSSACVLFILDEMRKKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 302

HSP 2 Score: 26.5646 bits (57), Expect = 3.225e-41
Identity = 19/43 (44.19%), Postives = 22/43 (51.16%), Query Frame = -1

Query:  540 PRETHLDSLVEQXXXXXXXXXLNGGF*SQFPEIEKTKFLKMVW 668
            P ETHLDSLV Q         +  G     PEIEK  F ++VW
Sbjct:  114 PSETHLDSLVGQALFGDGAAAIIVGS-DPVPEIEKPIF-ELVW 154

[Close]

BLAST of HO063057 vs. TrEMBL
Match: Q9ZRV7_CICAR (Chalcone synthase OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 3.428e-41
Identity = 105/166 (63.25%), Postives = 116/166 (69.88%), Query Frame = -3

Query:   88 PIPGN*ENQIFEDGLGRHKPIAPR*GKGAIDGHLWGKQDSHFPFLKMFRGLSQRTLIKL*LKLSTH*TYLFTIQYLGLHIQGGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 585
            PIP   E  IFE  +   + IAP   +GAIDGHL  +    F  LK   G+  + + K  ++               +   GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSS CVLFILDEMR+KSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI
Sbjct:  228 PIP-EIEKPIFEM-VWTAQTIAPD-SEGAIDGHL-REAGLTFHLLKDVPGIVSKNIDKALIEAFQPLNISDYNSIFWIAHPGGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSACVLFILDEMRKKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 389

[Close]

BLAST of HO063057 vs. TAIR peptide
Match: AT5G13930.1 (| Symbols: CHS, TT4, ATCHS | Chalcone and stilbene synthase family protein | chr5:4488762-4490035 FORWARD LENGTH=395)

HSP 1 Score: 155.992 bits (393), Expect = 1.744e-38
Identity = 93/145 (64.14%), Postives = 102/145 (70.34%), Query Frame = -3

Query:   88 GAIDGHLWGKQDSHFPFLKMFRGLSQRTLIKL*------LKLSTH*TYLFTIQYLGLHIQGGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 504
            GAIDGHL  +    F  LK   GL  + ++K        L +S     LF I +      GGPAILDQVE KL LK EKMRATR VLSEYGNMSS CVLFILDEMRRKSAKDG+ TTGEGLEWGVLFGFGPGLT+ETVVLHSV +
Sbjct:  258 GAIDGHL-REVGLTFHLLKDVPGLISKNIVKSLDEAFKPLGISDW-NSLFWIAH-----PGGPAILDQVEIKLGLKEEKMRATRHVLSEYGNMSSACVLFILDEMRRKSAKDGVATTGEGLEWGVLFGFGPGLTVETVVLHSVPL 395

[Close]

BLAST of HO063057 vs. TAIR peptide
Match: AT4G00040.1 (| Symbols: | Chalcone and stilbene synthase family protein | chr4:14653-15897 FORWARD LENGTH=385)

HSP 1 Score: 77.411 bits (189), Expect = 7.883e-15
Identity = 37/83 (44.58%), Postives = 54/83 (65.06%), Query Frame = -3

Query:   94 GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSV 342
            GGPAIL  +E KL LKPEK+  +R  L +YGN+SS  + +I+D++R +  K G     EG EWG+   FGPG+T E  ++ ++
Sbjct:  307 GGPAILSGLETKLKLKPEKLECSRRALMDYGNVSSNTIFYIMDKVRDELEKKGT----EGEEWGLGLAFGPGITFEGFLMRNL 385

[Close]

BLAST of HO063057 vs. TAIR peptide
Match: AT1G02050.1 (| Symbols: LAP6 | Chalcone and stilbene synthase family protein | chr1:359164-360441 REVERSE LENGTH=395)

HSP 1 Score: 73.1738 bits (178), Expect = 1.487e-13
Identity = 36/84 (42.86%), Postives = 57/84 (67.86%), Query Frame = -3

Query:   94 GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGL-EWGVLFGFGPGLTIETVVLHSV 342
            GGPAIL+++E KL L+ EK+ ++R  L +YGN+SS  +L++++ MR     D LK  G+   EWG+   FGPG+T E +++ S+
Sbjct:  314 GGPAILNRLETKLKLEKEKLESSRRALVDYGNVSSNTILYVMEYMR-----DELKKKGDAAQEWGLGLAFGPGITFEGLLIRSL 392

[Close]

BLAST of HO063057 vs. TAIR peptide
Match: AT4G34850.1 (| Symbols: LAP5 | Chalcone and stilbene synthase family protein | chr4:16608349-16609720 FORWARD LENGTH=392)

HSP 1 Score: 72.7886 bits (177), Expect = 1.942e-13
Identity = 33/85 (38.82%), Postives = 55/85 (64.71%), Query Frame = -3

Query:   88 GGPAILDQVEEKLALKPEKMRATREVLSEYGNMSSGCVLFILDEMRRKSAKDGLKTTGEGLEWGVLFGFGPGLTIETVVLHSVAI 342
            GGPAIL+++E++L L PEK+  +R  L +YGN SS  ++++L+ M  +S K       E  EWG++  FGPG+T E ++  ++ +
Sbjct:  309 GGPAILNRIEKRLNLSPEKLSPSRRALMDYGNASSNSIVYVLEYMLEESKKVRNMNEEEN-EWGLILAFGPGVTFEGIIARNLDV 392

The following BLAST results are available for this feature:

BLAST of HO063057 vs. SwissProt
BLAST of HO063057 vs. TrEMBL
BLAST of HO063057 vs. TAIR peptide

BLAST of HO063057 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
CHS3_CAMSI	2.206e-48	86.75	Chalcone synthase 3 OS=Camellia sinensis GN=CHS3 P...	[more]
CHS1_CICAR	1.043e-42	63.86	Chalcone synthase 1 OS=Cicer arietinum GN=CHS1 PE=...	[more]
CHSF_PETHY	1.667e-42	71.76	Chalcone synthase F OS=Petunia hybrida GN=CHSF PE=...	[more]
CHSG_PETHY	3.687e-42	77.65	Chalcone synthase G OS=Petunia hybrida GN=CHSG PE=...	[more]
CHS3_TRISU	8.158e-42	64.38	Chalcone synthase 3 OS=Trifolium subterraneum GN=C...	[more]
CHSY_EQUAR	1.193e-41	76.47	Chalcone synthase OS=Equisetum arvense GN=CHS PE=2...	[more]
CHS6_PEA	3.992e-41	63.70	Chalcone synthase 6 OS=Pisum sativum GN=CHS6 PE=2 ...	[more]
CHSD_PETHY	1.498e-40	71.43	Chalcone synthase D OS=Petunia hybrida GN=CHSD PE=...	[more]
CHS2_TRISU	2.174e-40	60.24	Chalcone synthase 2 OS=Trifolium subterraneum GN=C...	[more]
CHS4_PEA	2.545e-40	63.01	Chalcone synthase 4 OS=Pisum sativum GN=CHS4 PE=3 ...	[more]

BLAST of HO063057 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q7Y1X9_LUPLU	5.531e-51	91.57	Chalcone synthase OS=Lupinus luteus GN=CHS PE=2 SV...	[more]
Q460Q1_ARAGE	2.353e-48	88.24	Chalcone synthase family protein OS=Arabis gemmife...	[more]
Q690N1_CAMSI	3.269e-47	86.75	Chalcone synthase OS=Camellia sinensis GN=CHS PE=2...	[more]
Q2WBN0_9LAMI	9.697e-47	81.18	Chalcone synthase OS=Misopates orontium GN=chs PE=...	[more]
Q9FS37_9LAMI	1.647e-46	85.37	Chalcone synthase OS=Torenia hybrid cultivar PE=2 ...	[more]
Q6X3Y1_IPOTF	4.560e-46	88.46	Chalcone synthase (Fragment) OS=Ipomoea trifida GN...	[more]
Q6PYC2_CAMSI	8.281e-46	85.71	Chalcone synthase 1 (Fragment) OS=Camellia sinensi...	[more]
Q6PYC1_CAMSI	1.970e-44	86.75	Chalcone synthase 3 (Fragment) OS=Camellia sinensi...	[more]
B6S394_ROBPS	3.225e-41	66.91	Chalcone synthase (Fragment) OS=Robinia pseudoacac...	[more]
Q9ZRV7_CICAR	3.428e-41	63.25	Chalcone synthase OS=Cicer arietinum PE=2 SV=1	[more]

BLAST of HO063057 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT5G13930.1	1.744e-38	64.14	\| Symbols: CHS, TT4, ATCHS \| Chalcone and stilbene...	[more]
AT4G00040.1	7.883e-15	44.58	\| Symbols: \| Chalcone and stilbene synthase famil...	[more]
AT1G02050.1	1.487e-13	42.86	\| Symbols: LAP6 \| Chalcone and stilbene synthase f...	[more]
AT4G34850.1	1.942e-13	38.82	\| Symbols: LAP5 \| Chalcone and stilbene synthase f...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 47..62 score
None	No IPR available	SEG	seg	seg	coord: 20..41 score
None	No IPR available	SEG	seg	seg	coord: 40..51 score
None	No IPR available	SEG	seg	seg	coord: 13..24 score
IPR012328	Chalcone/stilbene synthase, C-terminal	PFAM	PF02797	Chal_sti_synt_C	coord: 14..97 score: 3.7
IPR012328	Chalcone/stilbene synthase, C-terminal	PFAM	PF02797	Chal_sti_synt_C	coord: 5..57 score: 3
IPR016038	Thiolase-like, subgroup	GENE3D	3.40.47.10	no description	coord: 14..97 score: 5.4
IPR016038	Thiolase-like, subgroup	GENE3D	3.40.47.10	no description	coord: 5..51 score: 1.6
IPR016039	Thiolase-like	SUPERFAMILY	53901	Thiolase-like	coord: 14..96 score: 7.2

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO063057 ID=HO063057; Name=HO063057; organism=Cicer arietinum; type=EST; length=680bp
CAAGTCCAAATGTTTATTCTTCATAGATGAAAACTCAAGCAAGATTGAAA
GTCATACAATGAAATAAAGTAACAATAAAACATTTCATATAGCCACACTG
TGTAGAACAACAGTTTCAATAGTAAGTCCTGGTCCAAATCCAAACAACAC
ACCCCATTCAAGTCCTTCTCCTGTTGTCTTCAGTCCATCTTTAGCTGATT
TCCTTCTCATTTCATCCAATATAAACAATACACACCCACTTGACATATTT
CCATATTCACTCAACACTTCCCTTGTTGCCCTCATCTTTTCAGGCTTCAA
GGCTAACTTTTCCTCTACTTGATCTAAAATTGCTGGTCCACCCTGGATGT
GCAATCCCAAATATTGAATTGTAAACAAATATGTTCAATGGGTGGAAAGC
TTCAATCAAAGCTTTATCAATGTTCTTTGAGACAATCCCCGGAACATCTT
TAAAAAGGGGAAATGTGAGTCCTGCTTTCCCCAAAGGTGACCATCAATAG
CACCTTTCCCCTATCTGGGAGCAATTGGTTTGTGCCGNCCCAAACCATCT
TCAAAAATTTGGTTTTCTCAATTTCCGGGAATTGGGATCAAAACCCACCA
TTCAATGCAGCAGCCTCCTCCTCCAAAAAAAGTTGTTCAACAAGACTATC
CAAATGAGTTTCCCTTGGAACCACCAAAGG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008415	acyltransferase activity (GO:0008415)
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR012328	Chalcone/stilbene_synt_C
IPR016038	Thiolase-like, subgroup
IPR016039	Thiolase-like

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