GR408866

EST Overview


Name	GR408866
Unique Name	GR408866
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	682

Libraries


Library Name	Type
LIBEST_024777 ICC1882 dehydration stressed root cDNA library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of GR408866 vs. SwissProt
Match: MCCB_ARATH (Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial OS=Arabidopsis thaliana GN=MCCB PE=2 SV=1)

HSP 1 Score: 284.263 bits (726), Expect = 4.194e-76
Identity = 134/179 (74.86%), Postives = 153/179 (85.47%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFASLNRAPENTKYGVFRM 680
            AHFIE+C+QR IPLVFLQNITGFMVGSR+EANGIAK GAKMVMAVSCAKVPKITII G SFGAGNYAMCGRAYSP+FMF+WPNARI +MGGAQAAGVL QIE+  KK+QGI+W             +AYERE +PYYSTARLWDDG+IDP DTRK++GLC+ A+LNR  E+T++GVFRM
Sbjct:  409 AHFIELCSQRKIPLVFLQNITGFMVGSRAEANGIAKAGAKMVMAVSCAKVPKITIITGASFGAGNYAMCGRAYSPDFMFIWPNARIGIMGGAQAAGVLTQIERATKKRQGIKWTEEEEEAFKKKTVDAYEREANPYYSTARLWDDGVIDPCDTRKVLGLCLSAALNRPLEDTRFGVFRM 587

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BLAST of GR408866 vs. SwissProt
Match: MCCB_DROME (Probable methylcrotonoyl-CoA carboxylase beta chain, mitochondrial OS=Drosophila melanogaster GN=L(2)04524 PE=2 SV=1)

HSP 1 Score: 246.128 bits (627), Expect = 1.266e-64
Identity = 117/179 (65.36%), Postives = 143/179 (79.89%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFASLNRAPENTKYGVFRM 680
            AHFI++C QR IPLVFLQNITGFMVG  +EANGIAK GAKMV AV+CA VPK T+I+GGS+GAGNY MCGRAYSP F+++WPN+RISVMGG QAA V+AQI +  +K+ G +++            E +E EGSPYYSTARLWDDGIIDPA+TR+I+GL + A+LN A + TK+GVFRM
Sbjct:  400 AHFIQLCAQRKIPLVFLQNITGFMVGRDAEANGIAKNGAKMVTAVACANVPKFTVIIGGSYGAGNYGMCGRAYSPRFLYMWPNSRISVMGGTQAANVMAQITEDQRKRAGKEFSEEEAQKLKAPIVEMFEAEGSPYYSTARLWDDGIIDPANTRQILGLSLKAALNNAGQETKFGVFRM 578

[Close]

BLAST of GR408866 vs. SwissProt
Match: MCCB_RAT (Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial OS=Rattus norvegicus GN=Mccc2 PE=2 SV=1)

HSP 1 Score: 238.81 bits (608), Expect = 2.021e-62
Identity = 112/179 (62.57%), Postives = 139/179 (77.65%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFASLNRAPENTKYGVFRM 680
            AHF+++C QRNIPL+FLQNITGFMVG   EA GIAK GAKMV AVSCAKVPKIT+I+GGS+GAGNY MCGRAYSP F+++WPNARISVMGG QAA VLA + +  + ++G Q++            + +E EG+PYYS+ARLWDDGIIDP DTR ++GL I A+LN   + T +G+FRM
Sbjct:  385 AHFVQLCCQRNIPLLFLQNITGFMVGKDYEAEGIAKDGAKMVAAVSCAKVPKITVIIGGSYGAGNYGMCGRAYSPRFLYMWPNARISVMGGEQAATVLATVARDQRAREGKQFSSAEEAALKEPIIKRFEEEGNPYYSSARLWDDGIIDPVDTRLVLGLSISAALNAPIQRTDFGIFRM 563

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BLAST of GR408866 vs. SwissProt
Match: MCCB_MOUSE (Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial OS=Mus musculus GN=Mccc2 PE=2 SV=1)

HSP 1 Score: 237.654 bits (605), Expect = 4.501e-62
Identity = 111/179 (62.01%), Postives = 139/179 (77.65%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFASLNRAPENTKYGVFRM 680
            AHF+++C QRNIPL+FLQNITGFMVG   EA GIAK GAKMV AV+CAKVPKIT+I+GGS+GAGNY MCGRAYSP F+++WPNARISVMGG QAA VLA + +  K ++G Q++            + +E EG+PYYS+ARLWDDGIIDP DTR ++GL + A+LN   + T +G+FRM
Sbjct:  385 AHFVQLCCQRNIPLLFLQNITGFMVGRDYEAEGIAKDGAKMVAAVACAKVPKITVIIGGSYGAGNYGMCGRAYSPRFLYMWPNARISVMGGEQAATVLATVARDQKAREGKQFSSAEEAALKEPIIKRFEEEGNPYYSSARLWDDGIIDPVDTRLVLGLSLSAALNAPIQRTDFGIFRM 563

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BLAST of GR408866 vs. SwissProt
Match: MCCB_HUMAN (Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial OS=Homo sapiens GN=MCCC2 PE=1 SV=1)

HSP 1 Score: 236.884 bits (603), Expect = 7.678e-62
Identity = 111/178 (62.36%), Postives = 138/178 (77.53%), Query Frame = -3

Query:  144 HFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFASLNRAPENTKYGVFRM 677
            HF+++C QRNIPL+FLQNITGFMVG   EA GIAK GAKMV AV+CA+VPKIT+I+GGS+GAGNY MCGRAYSP F+++WPNARISVMGG QAA VLA I K  + ++G Q++            + +E EG+PYYS+AR+WDDGIIDPADTR ++GL   A+LN   E T +G+FRM
Sbjct:  386 HFVQLCCQRNIPLLFLQNITGFMVGREYEAEGIAKDGAKMVAAVACAQVPKITLIIGGSYGAGNYGMCGRAYSPRFLYIWPNARISVMGGEQAANVLATITKDQRAREGKQFSSADEAALKEPIIKKFEEEGNPYYSSARVWDDGIIDPADTRLVLGLSFSAALNAPIEKTDFGIFRM 563

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BLAST of GR408866 vs. SwissProt
Match: MCCB_CAEEL (Probable methylcrotonoyl-CoA carboxylase beta chain, mitochondrial OS=Caenorhabditis elegans GN=F02A9.4 PE=2 SV=1)

HSP 1 Score: 236.884 bits (603), Expect = 7.678e-62
Identity = 108/179 (60.34%), Postives = 138/179 (77.09%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFASLNRAPENTKYGVFRM 680
            +HFIE+C QR IPL+FLQNITGFMVG  +EA GIAK GAK+V AV+CAKVPKIT++VGGS+GAGNY MCGR YSP ++F+WPN+RISVMGG QAA VL+ ++K  KK++G  W             E +E+EG PY+++ARLWDDG+IDP DTRK++GL   ++L +    TK+GVFRM
Sbjct:  430 SHFIELCCQRKIPLLFLQNITGFMVGRDAEAGGIAKHGAKLVTAVACAKVPKITVLVGGSYGAGNYGMCGRGYSPRYVFMWPNSRISVMGGEQAANVLSTVQKEKKKREGADWTDQQDLELRKPVEEKFEKEGHPYFASARLWDDGVIDPKDTRKVLGLAFQSTLQKPIPETKFGVFRM 608

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BLAST of GR408866 vs. SwissProt
Match: MCCB_DICDI (Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial OS=Dictyostelium discoideum GN=mccb PE=3 SV=2)

HSP 1 Score: 232.261 bits (591), Expect = 1.891e-60
Identity = 112/181 (61.88%), Postives = 137/181 (75.69%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFASLNRA--PENTKYGVFRM 680
            AHFIE+C QR IPLVFLQNITGFMVG   E+ GIAK GAKMVMAV+ AKVPKIT+I+GGSFGAGNY MCGR+YSP F+++WPNA+ISVMGG QAA VLAQI+K N  K+  QW+            + +E EGS YYS+AR WDDG+IDP D+RK++ L + A +N+   P +  +GVFRM
Sbjct:  408 AHFIELCNQRGIPLVFLQNITGFMVGKTYESKGIAKDGAKMVMAVATAKVPKITMIIGGSFGAGNYGMCGRSYSPRFLYMWPNAKISVMGGEQAASVLAQIQKDNMAKENKQWSPEEENTFKKPISDKFEEEGSIYYSSARCWDDGVIDPQDSRKVIALSLSACMNQPINPPSDGFGVFRM 588

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BLAST of GR408866 vs. SwissProt
Match: 12S_PROFR (Methylmalonyl-CoA carboxyltransferase 12S subunit OS=Propionibacterium freudenreichii subsp. shermanii PE=1 SV=3)

HSP 1 Score: 95.1301 bits (235), Expect = 3.608e-19
Identity = 57/155 (36.77%), Postives = 81/155 (52.26%), Query Frame = -3

Query:  216 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRK 680
            A F+  C   NIPLV L ++ GF+ G + E  GI + GAKM+ A S A VPKIT+++  ++G    AMC R    + ++ WP+A I+VMG   AA V+ + E        I+              E      +PY + AR   D +IDPADTR+
Sbjct:  352 AEFVNFCDSFNIPLVQLVDVPGFLPGVQQEYGGIIRHGAKMLYAYSEATVPKITVVLRKAYGGSYLAMCNRDLGADAVYAWPSAEIAVMGAEGAANVIFRKE--------IKAADDPDAMRAEKIEEYQNAFNTPYVAAARGQVDDVIDPADTRR 498

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BLAST of GR408866 vs. SwissProt
Match: YNGE_BACSU (Uncharacterized carboxylase yngE OS=Bacillus subtilis GN=yngE PE=3 SV=2)

HSP 1 Score: 90.1225 bits (222), Expect = 1.161e-17
Identity = 45/98 (45.92%), Postives = 65/98 (66.33%), Query Frame = -3

Query:  387 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVL 680
            A FI +C   +IPL+FL +I GFM+G++ E  GI + GAKM+ A+S A VPK+++IV  ++GAG YAM G A+ P+     P A+I+VMG   A   +
Sbjct:  338 AKFITLCDAFHIPLLFLADIPGFMIGTKVEQAGIIRHGAKMISAMSEATVPKLSVIVRKAYGAGLYAMAGPAFEPDCCLALPTAQIAVMGPEAAVNAV 435

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BLAST of GR408866 vs. SwissProt
Match: PCCB_BACSU (Putative propionyl-CoA carboxylase beta chain OS=Bacillus subtilis GN=yqjD PE=3 SV=2)

HSP 1 Score: 85.1149 bits (209), Expect = 3.734e-16
Identity = 41/102 (40.20%), Postives = 64/102 (62.75%), Query Frame = -3

Query:  375 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIE 680
            A FI  C   +IPL+ ++++ GF+ G + E NGI + GAK++ A + A VPK+T+I+  ++G    AM  +A   + +F WPNA I+VMG   AA +L + E
Sbjct:  335 ARFIRFCDAFDIPLLTVEDVPGFLPGIQQEHNGIIRHGAKLLFAYAEATVPKVTLIIRKAYGGAYVAMNSKAIGADLVFAWPNAEIAVMGPEGAASILYEKE 436

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BLAST of GR408866 vs. TrEMBL
Match: D7TPH5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_63.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00031728001 PE=4 SV=1)

HSP 1 Score: 308.531 bits (789), Expect = 3.065e-82
Identity = 155/180 (86.11%), Postives = 161/180 (89.44%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFA-SLNRAPENTKYGVFRM 680
            AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAK GAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVL+QIE+GNKKKQGIQW             EAYE+EGS YYSTARLWDDGIIDPADTRKI+GLCI A + N APE+TKYGVFRM
Sbjct:  397 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKSGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLSQIERGNKKKQGIQWPKDEEEAFKAKVVEAYEKEGSSYYSTARLWDDGIIDPADTRKIIGLCISATTTNHAPEDTKYGVFRM 576

[Close]

BLAST of GR408866 vs. TrEMBL
Match: A5AX02_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_001775 PE=4 SV=1)

HSP 1 Score: 308.531 bits (789), Expect = 3.065e-82
Identity = 155/180 (86.11%), Postives = 161/180 (89.44%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFA-SLNRAPENTKYGVFRM 680
            AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAK GAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVL+QIE+GNKKKQGIQW             EAYE+EGS YYSTARLWDDGIIDPADTRKI+GLCI A + N APE+TKYGVFRM
Sbjct:  391 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKSGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLSQIERGNKKKQGIQWPKEEEEAFKAKVVEAYEKEGSSYYSTARLWDDGIIDPADTRKIIGLCISATTTNHAPEDTKYGVFRM 570

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BLAST of GR408866 vs. TrEMBL
Match: B9HS16_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_557395 PE=4 SV=1)

HSP 1 Score: 301.212 bits (770), Expect = 4.894e-80
Identity = 148/179 (82.68%), Postives = 157/179 (87.71%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFASLNRAPENTKYGVFRM 680
            AHFIE+CTQRNIPLVFLQNITGFMVGSRSEANGIAK GAKMVMAVSCAKVPK+TI+VGGSFGAGNYAMCGRAYSPNF+FLWPNA+ISVMGGAQAA VL+QIE  NKKKQGIQW             EAYEREG+ YYSTARLWDDGIIDPADTRKI+G CI ASLNR  E+TKYGVFRM
Sbjct:  395 AHFIELCTQRNIPLVFLQNITGFMVGSRSEANGIAKSGAKMVMAVSCAKVPKVTIMVGGSFGAGNYAMCGRAYSPNFLFLWPNAKISVMGGAQAASVLSQIETANKKKQGIQWTEEEQENFKSKITEAYEREGNCYYSTARLWDDGIIDPADTRKIIGFCISASLNRPSEDTKYGVFRM 573

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BLAST of GR408866 vs. TrEMBL
Match: C5YL06_SORBI (Putative uncharacterized protein Sb07g020640 OS=Sorghum bicolor GN=Sb07g020640 PE=4 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 1.924e-76
Identity = 137/179 (76.54%), Postives = 155/179 (86.59%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFASLNRAPENTKYGVFRM 680
            +HFIE+C QR+IPL+FLQNITGFMVGS+SEA+GIAK GAKMVMAVSCAKVPKITIIVGGSFGAGNY MCGRAYSPNF+FLWP ARISVMGG QAAGVLAQIEK NKK+QG++W+            EAY+REGSPYYSTARLWDDG+IDPADTR+++ LC+ AS    PE+TKYGVFRM
Sbjct:  407 SHFIELCAQRHIPLIFLQNITGFMVGSKSEASGIAKAGAKMVMAVSCAKVPKITIIVGGSFGAGNYGMCGRAYSPNFLFLWPTARISVMGGIQAAGVLAQIEKNNKKRQGVEWSKDEEEAFKAKVVEAYDREGSPYYSTARLWDDGVIDPADTRRVLSLCLSASAKPVPEDTKYGVFRM 585

[Close]

BLAST of GR408866 vs. TrEMBL
Match: B6SMD3_MAIZE (Propionyl-CoA carboxylase beta chain OS=Zea mays PE=2 SV=1)

HSP 1 Score: 288.115 bits (736), Expect = 4.287e-76
Identity = 136/179 (75.98%), Postives = 155/179 (86.59%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFASLNRAPENTKYGVFRM 680
            +HFIE+C QR+IPL+FLQNITGFMVGS+SEA+GIAK GAKMVMAVSCAKVPKITIIVGGSFGAGNY MCGRAYSPNF+FLWP ARISVMGG QAAGVLAQIEK NKK+QG++W+            EAY+REGSPYYSTARLWDDG+IDPADTR+++ LC+ AS    PE+T+YGVFRM
Sbjct:  397 SHFIELCAQRHIPLIFLQNITGFMVGSKSEASGIAKAGAKMVMAVSCAKVPKITIIVGGSFGAGNYGMCGRAYSPNFLFLWPTARISVMGGIQAAGVLAQIEKNNKKRQGVEWSKDEEEAFKAKVVEAYDREGSPYYSTARLWDDGVIDPADTRRVLSLCLSASAKPVPEDTRYGVFRM 575

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BLAST of GR408866 vs. TrEMBL
Match: C0P9G2_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 286.96 bits (733), Expect = 9.550e-76
Identity = 135/179 (75.42%), Postives = 155/179 (86.59%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFASLNRAPENTKYGVFRM 680
            +HFIE+C QR+IPL+FLQNITGFMVGS+SEA+GIAK GAKMVMAVSCAKVPKITIIVGGSFGAGNY MCGRAYSPNF+FLWP ARISVMGG QAAGVLAQIEK NK++QG++W+            EAY+REGSPYYSTARLWDDG+IDPADTR+++ LC+ AS    PE+T+YGVFRM
Sbjct:  257 SHFIELCAQRHIPLIFLQNITGFMVGSKSEASGIAKAGAKMVMAVSCAKVPKITIIVGGSFGAGNYGMCGRAYSPNFLFLWPTARISVMGGIQAAGVLAQIEKNNKRRQGVEWSKDEEEAFKAKVVEAYDREGSPYYSTARLWDDGVIDPADTRRVLSLCLSASAKPVPEDTRYGVFRM 435

[Close]

BLAST of GR408866 vs. TrEMBL
Match: B6SWZ4_MAIZE (Propionyl-CoA carboxylase beta chain OS=Zea mays PE=2 SV=1)

HSP 1 Score: 286.96 bits (733), Expect = 9.550e-76
Identity = 135/179 (75.42%), Postives = 155/179 (86.59%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFASLNRAPENTKYGVFRM 680
            +HFIE+C QR+IPL+FLQNITGFMVGS+SEA+GIAK GAKMVMAVSCAKVPKITIIVGGSFGAGNY MCGRAYSPNF+FLWP ARISVMGG QAAGVLAQIEK NK++QG++W+            EAY+REGSPYYSTARLWDDG+IDPADTR+++ LC+ AS    PE+T+YGVFRM
Sbjct:  400 SHFIELCAQRHIPLIFLQNITGFMVGSKSEASGIAKAGAKMVMAVSCAKVPKITIIVGGSFGAGNYGMCGRAYSPNFLFLWPTARISVMGGIQAAGVLAQIEKNNKRRQGVEWSKDEEEAFKAKVVEAYDREGSPYYSTARLWDDGVIDPADTRRVLSLCLSASAKPVPEDTRYGVFRM 578

[Close]

BLAST of GR408866 vs. TrEMBL
Match: Q6YZ75_ORYSJ (Putative methylcrotonyl-CoA carboxylase beta chain, mitochondrial OS=Oryza sativa subsp. japonica GN=OSJNBa0056L09.27 PE=4 SV=1)

HSP 1 Score: 285.804 bits (730), Expect = 2.128e-75
Identity = 134/179 (74.86%), Postives = 154/179 (86.03%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFASLNRAPENTKYGVFRM 680
            +HFIE+C QRNIPL+FLQNITGFMVGS+SEA+GIAK GAKMVMAVSC+KVPKITIIVGGSFGAGNY MCGRAYSPNF+F+WP ARISVMGG QAAGVLAQIEK N+K+QG++W             EAY++EGSPYYSTARLWDDGIIDPADTR+++ LC+ A+    PE+TKYGVFRM
Sbjct:  394 SHFIELCAQRNIPLIFLQNITGFMVGSKSEASGIAKAGAKMVMAVSCSKVPKITIIVGGSFGAGNYGMCGRAYSPNFLFMWPTARISVMGGIQAAGVLAQIEKNNRKRQGVEWTKDEEEAFKAKVVEAYDKEGSPYYSTARLWDDGIIDPADTRRVLSLCLSAAAKPVPEDTKYGVFRM 572

[Close]

BLAST of GR408866 vs. TrEMBL
Match: Q0J5L9_ORYSJ (Os08g0424200 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os08g0424200 PE=4 SV=1)

HSP 1 Score: 285.804 bits (730), Expect = 2.128e-75
Identity = 134/179 (74.86%), Postives = 154/179 (86.03%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFASLNRAPENTKYGVFRM 680
            +HFIE+C QRNIPL+FLQNITGFMVGS+SEA+GIAK GAKMVMAVSC+KVPKITIIVGGSFGAGNY MCGRAYSPNF+F+WP ARISVMGG QAAGVLAQIEK N+K+QG++W             EAY++EGSPYYSTARLWDDGIIDPADTR+++ LC+ A+    PE+TKYGVFRM
Sbjct:  132 SHFIELCAQRNIPLIFLQNITGFMVGSKSEASGIAKAGAKMVMAVSCSKVPKITIIVGGSFGAGNYGMCGRAYSPNFLFMWPTARISVMGGIQAAGVLAQIEKNNRKRQGVEWTKDEEEAFKAKVVEAYDKEGSPYYSTARLWDDGIIDPADTRRVLSLCLSAAAKPVPEDTKYGVFRM 310

[Close]

BLAST of GR408866 vs. TrEMBL
Match: B9G0Z1_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_27365 PE=4 SV=1)

HSP 1 Score: 285.804 bits (730), Expect = 2.128e-75
Identity = 134/179 (74.86%), Postives = 154/179 (86.03%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFASLNRAPENTKYGVFRM 680
            +HFIE+C QRNIPL+FLQNITGFMVGS+SEA+GIAK GAKMVMAVSC+KVPKITIIVGGSFGAGNY MCGRAYSPNF+F+WP ARISVMGG QAAGVLAQIEK N+K+QG++W             EAY++EGSPYYSTARLWDDGIIDPADTR+++ LC+ A+    PE+TKYGVFRM
Sbjct:  378 SHFIELCAQRNIPLIFLQNITGFMVGSKSEASGIAKAGAKMVMAVSCSKVPKITIIVGGSFGAGNYGMCGRAYSPNFLFMWPTARISVMGGIQAAGVLAQIEKNNRKRQGVEWTKDEEEAFKAKVVEAYDKEGSPYYSTARLWDDGIIDPADTRRVLSLCLSAAAKPVPEDTKYGVFRM 556

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BLAST of GR408866 vs. TAIR peptide
Match: AT4G34030.1 (| Symbols: MCCB | 3-methylcrotonyl-CoA carboxylase | chr4:16301298-16303949 FORWARD LENGTH=587)

HSP 1 Score: 284.263 bits (726), Expect = 4.246e-77
Identity = 134/179 (74.86%), Postives = 153/179 (85.47%), Query Frame = -3

Query:  144 AHFIEICTQRNIPLVFLQNITGFMVGSRSEANGIAKFGAKMVMAVSCAKVPKITIIVGGSFGAGNYAMCGRAYSPNFMFLWPNARISVMGGAQAAGVLAQIEKGNKKKQGIQWNXXXXXXXXXXXXEAYEREGSPYYSTARLWDDGIIDPADTRKIVGLCIFASLNRAPENTKYGVFRM 680
            AHFIE+C+QR IPLVFLQNITGFMVGSR+EANGIAK GAKMVMAVSCAKVPKITII G SFGAGNYAMCGRAYSP+FMF+WPNARI +MGGAQAAGVL QIE+  KK+QGI+W             +AYERE +PYYSTARLWDDG+IDP DTRK++GLC+ A+LNR  E+T++GVFRM
Sbjct:  409 AHFIELCSQRKIPLVFLQNITGFMVGSRAEANGIAKAGAKMVMAVSCAKVPKITIITGASFGAGNYAMCGRAYSPDFMFIWPNARIGIMGGAQAAGVLTQIERATKKRQGIKWTEEEEEAFKKKTVDAYEREANPYYSTARLWDDGVIDPCDTRKVLGLCLSAALNRPLEDTRFGVFRM 587

The following BLAST results are available for this feature:

BLAST of GR408866 vs. SwissProt
BLAST of GR408866 vs. TrEMBL
BLAST of GR408866 vs. TAIR peptide

BLAST of GR408866 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
MCCB_ARATH	4.194e-76	74.86	Methylcrotonoyl-CoA carboxylase beta chain, mitoch...	[more]
MCCB_DROME	1.266e-64	65.36	Probable methylcrotonoyl-CoA carboxylase beta chai...	[more]
MCCB_RAT	2.021e-62	62.57	Methylcrotonoyl-CoA carboxylase beta chain, mitoch...	[more]
MCCB_MOUSE	4.501e-62	62.01	Methylcrotonoyl-CoA carboxylase beta chain, mitoch...	[more]
MCCB_HUMAN	7.678e-62	62.36	Methylcrotonoyl-CoA carboxylase beta chain, mitoch...	[more]
MCCB_CAEEL	7.678e-62	60.34	Probable methylcrotonoyl-CoA carboxylase beta chai...	[more]
MCCB_DICDI	1.891e-60	61.88	Methylcrotonoyl-CoA carboxylase beta chain, mitoch...	[more]
12S_PROFR	3.608e-19	36.77	Methylmalonyl-CoA carboxyltransferase 12S subunit ...	[more]
YNGE_BACSU	1.161e-17	45.92	Uncharacterized carboxylase yngE OS=Bacillus subti...	[more]
PCCB_BACSU	3.734e-16	40.20	Putative propionyl-CoA carboxylase beta chain OS=B...	[more]

BLAST of GR408866 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7TPH5_VITVI	3.065e-82	86.11	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5AX02_VITVI	3.065e-82	86.11	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9HS16_POPTR	4.894e-80	82.68	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
C5YL06_SORBI	1.924e-76	76.54	Putative uncharacterized protein Sb07g020640 OS=So...	[more]
B6SMD3_MAIZE	4.287e-76	75.98	Propionyl-CoA carboxylase beta chain OS=Zea mays P...	[more]
C0P9G2_MAIZE	9.550e-76	75.42	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B6SWZ4_MAIZE	9.550e-76	75.42	Propionyl-CoA carboxylase beta chain OS=Zea mays P...	[more]
Q6YZ75_ORYSJ	2.128e-75	74.86	Putative methylcrotonyl-CoA carboxylase beta chain...	[more]
Q0J5L9_ORYSJ	2.128e-75	74.86	Os08g0424200 protein (Fragment) OS=Oryza sativa su...	[more]
B9G0Z1_ORYSJ	2.128e-75	74.86	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of GR408866 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G34030.1	4.246e-77	74.86	\| Symbols: MCCB \| 3-methylcrotonyl-CoA carboxylase...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000022	Carboxyl transferase	PFAM	PF01039	Carboxyl_trans	coord: 1..166 score: 2.9
IPR011763	Acetyl-coenzyme A carboxyltransferase, C-terminal	PROFILE	PS50989	COA_CT_CTER	coord: 1..173 score: 35
None	No IPR available	GENE3D	3.90.226.10	no description	coord: 1..170 score: 1.1
None	No IPR available	PANTHER	PTHR22855	ACETYL, PROPIONYL, PYRUVATE, AND GLUTACONYL CARBOXYLASE-RELATED	coord: 1..179 score: 9.3e
None	No IPR available	PANTHER	PTHR22855:SF13	ACETYL-COA CARBOXYLASE CARBOXYLTRANSFERASE-RELATED	coord: 1..179 score: 9.3e
None	No IPR available	SEG	seg	seg	coord: 115..127 score
None	No IPR available	SUPERFAMILY	52096	ClpP/crotonase	coord: 1..174 score: 1.1

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GR408866 ID=GR408866; Name=GR408866; organism=Cicer arietinum; type=EST; length=682bp
CAGCCAAGAAGCATTTTTATTATAAATGGGGTTGTTACAGGGGGATGAAT
AACGATTTCACTTTTCACTCATTCGCTTTCTTAAATCACTAAAAAATCTA
AATCAAACTTCAAAGGGCACAAAACAACACAGATTCAACATTACATTCTA
AATACACCATATTTGGTATTTTCTGGGGCACGGTTCAGGGAAGCAAAGAT
ACATAGACCAACGATTTTTCTTGTATCAGCTGGATCAATTATTCCATCAT
CCCAAAGCCTTGCTGTTGAGTAATAAGGACTTCCTTCTCTCTCGTAAGCC
TCAACCACTTTTGTTTTGAACTTTTCCTCTTCTTCCTTGTTCCACTGAAT
TCCTTGCTTTTTCTTATTGCCTTTCTCTATTTGAGCCAAAACACCAGCAG
CCTGAGCACCACCCATCACAGATATTCTGGCATTAGGCCAAAGGAACATG
AAGTTCGGACTATAGGCACGACCGCACATTGCATAGTTTCCAGCACCAAA
GCTACCACCAACAATTATAGTGATTTTAGGTACCTTTGCGCAAGATACAG
CCATCACCATTTTTGCACCAAACTTTGCTATGCCATTTGCCTCGGATCTT
GAACCCACCATGAATCCAGTGATGTTTTGAAGGAAGACCAAAGGAATGTT
ACGCTGAGTACATATTTCAATGAAATGGGCCC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016874	ligase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR000022	Carboxyl_trans
IPR011763	COA_CT_C

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