FL512446

EST Overview


Name	FL512446
Unique Name	FL512446
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	513

Libraries


Library Name	Type
LIBEST_023567 Subtracted cDNA library of drought-stressed chickpea cultivar	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of FL512446 vs. SwissProt
Match: LEUC_ARATH (3-isopropylmalate dehydratase OS=Arabidopsis thaliana GN=IIL1 PE=2 SV=1)

HSP 1 Score: 178.333 bits (451), Expect = 1.808e-44
Identity = 86/108 (79.63%), Postives = 98/108 (90.74%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            AFGQFATGIGNTDAGFVLG GK+LLKVPPT+RF++DGEMP+YL AKDLILQIIGEISVAGA YK+MEF G+T+ESL+MEERMTL NMVVEAGG NG IPP++ T  Y+
Sbjct:  207 AFGQFATGIGNTDAGFVLGTGKILLKVPPTMRFILDGEMPSYLQAKDLILQIIGEISVAGATYKTMEFSGTTIESLSMEERMTLCNMVVEAGGKNGVIPPDATTLNYV 314

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BLAST of FL512446 vs. SwissProt
Match: LEUC_HALOH (3-isopropylmalate dehydratase large subunit OS=Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) GN=leuC PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 8.437e-26
Identity = 54/108 (50.00%), Postives = 75/108 (69.44%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            A G FATG+G+TD    +  G    KVP T++FV +GE+  ++  KDLIL  IG+I V GA+Y++MEF G T+E+L+M+ RMT+ NM +EAGG  G I P+  T+KYL
Sbjct:  131 ALGAFATGVGSTDMAAAMATGYTWFKVPSTIQFVYNGELKPWVSGKDLILYTIGDIGVDGALYRAMEFTGETIENLSMDNRMTISNMAIEAGGKCGLIAPDEKTFKYL 238

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BLAST of FL512446 vs. SwissProt
Match: LEUC_SYNAS (3-isopropylmalate dehydratase large subunit OS=Syntrophus aciditrophicus (strain SB) GN=leuC PE=3 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 9.328e-25
Identity = 53/108 (49.07%), Postives = 73/108 (67.59%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            A G FATG+G+TD    +  G++  KVP ++RFV+ G +  ++  KDLIL IIG+I V GA+YK+MEF G T+ SL M +R T+ NM +EAGG NG   P+ IT  Y+
Sbjct:  131 ALGAFATGVGSTDLAAAMLTGEVWFKVPESIRFVLSGTLNPWVSGKDLILYIIGKIGVDGALYKAMEFTGDTIASLTMADRFTIANMAIEAGGKNGIFTPDEITEAYV 238

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BLAST of FL512446 vs. SwissProt
Match: LEUC_WOLSU (3-isopropylmalate dehydratase large subunit OS=Wolinella succinogenes GN=leuC PE=3 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.218e-24
Identity = 53/111 (47.75%), Postives = 78/111 (70.27%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYLAGQ 336
            A G FATG+G+TD  + +  GK   KVPP++R V  G++  ++  KDLIL+II +I V GA+YK++EF G T+E L+M++R +L NM +EAG  NG I  +SIT ++LA +
Sbjct:  130 ALGAFATGMGSTDLAYAMITGKNWFKVPPSIRVVFKGKLQEHVYGKDLILEIIRQIGVDGALYKALEFQGDTIEQLSMDDRFSLCNMAIEAGAKNGIIAADSITKEFLASR 240

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BLAST of FL512446 vs. SwissProt
Match: LEUC1_PYRAB (3-isopropylmalate dehydratase large subunit 1 OS=Pyrococcus abyssi GN=leuC1 PE=3 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.218e-24
Identity = 51/108 (47.22%), Postives = 72/108 (66.67%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            A G FATG+G+TD    +  G+   +VP T++F+ +GE+  Y+  KDLIL  IG+I V GA+YK MEF GS +E L++E+RMT+ NM +EAG   G I P+  T  Y+
Sbjct:  131 ALGAFATGVGSTDLAVAMATGEAWFRVPETMKFIYEGELQPYVTGKDLILHTIGDIGVNGALYKVMEFSGSVIEELSVEQRMTMSNMAIEAGAKTGIIEPDKKTLDYV 238

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BLAST of FL512446 vs. SwissProt
Match: LEUC_AQUAE (3-isopropylmalate dehydratase large subunit OS=Aquifex aeolicus GN=leuC PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.591e-24
Identity = 48/108 (44.44%), Postives = 78/108 (72.22%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            A G FATG+G+TD  + +  G++ L+VP +++F+  G++  +++ KDLIL  IG+I V GA+Y++MEF G T+ +L+ME+R T+ NM +EAGG +G I P+  T +Y+
Sbjct:  130 ALGAFATGVGSTDIAYAMATGEVWLRVPESMKFIFYGKLKPWVMGKDLILYTIGQIGVDGALYRAMEFDGETIRNLSMEQRFTIANMAIEAGGKSGIISPDEKTIEYV 237

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BLAST of FL512446 vs. SwissProt
Match: LEUC1_PYRFU (3-isopropylmalate dehydratase large subunit 1 OS=Pyrococcus furiosus GN=leuC1 PE=3 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 4.629e-24
Identity = 50/111 (45.05%), Postives = 75/111 (67.57%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYLAGQ 336
            A G FATG+G+TD    +  G+   +VP T++FV +G++  ++ +KDLIL  IG+I V GA+YK MEF G  +E L++E+RMT+ NM +EAG   G I P+  T +Y+ G+
Sbjct:  132 ALGAFATGVGSTDLAVAMATGEAWFRVPETIKFVYEGDLQPWVTSKDLILYTIGDIGVNGALYKVMEFSGEVIEKLSVEQRMTMTNMAIEAGAKTGIIEPDKKTIEYVKGR 242

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BLAST of FL512446 vs. SwissProt
Match: LEUC_CLOPH (3-isopropylmalate dehydratase large subunit OS=Clostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) GN=leuC PE=3 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 6.046e-24
Identity = 50/108 (46.30%), Postives = 73/108 (67.59%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            A G F+TG+G+TD G  +  GK   KVP  ++F++ GE   ++  KD+IL IIG I V GA+YKSMEFVG+ +++L M++R T+ NM +EAG  NG  P + +T  Y+
Sbjct:  131 ALGAFSTGVGSTDMGAGMITGKAWFKVPAAIKFILTGEPKEWVSGKDVILHIIGMIGVDGALYKSMEFVGAGIKNLTMDDRFTIANMAIEAGAKNGIFPVDDLTISYM 238

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BLAST of FL512446 vs. SwissProt
Match: LEUC_METJA (Isopropylmalate/citramalate isomerase large subunit OS=Methanocaldococcus jannaschii GN=leuC PE=1 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 1.347e-23
Identity = 52/108 (48.15%), Postives = 72/108 (66.67%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            AFG FATGIG+TD   V   GKL  KVP T+ F + G++  Y+ +KD+IL IIGE+ V GA YK+ +F G TV+ +++  RMT+ NM +E GG  G I P+  T +Y+
Sbjct:  130 AFGAFATGIGSTDMAHVFATGKLWFKVPETIYFNITGDLQPYVTSKDVILSIIGEVGVDGATYKACQFGGETVKKMSIASRMTMTNMAIEMGGKTGIIEPDEKTIQYV 237

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BLAST of FL512446 vs. SwissProt
Match: LEUC_HELMI (3-isopropylmalate dehydratase large subunit OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) GN=leuC PE=3 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 1.347e-23
Identity = 51/108 (47.22%), Postives = 72/108 (66.67%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            A G FATG+G+TD    +  G+   KVP  ++F  +G++P  +  KDLIL  IG+I V GA+Y+SMEF GS ++ L+M+ RMT+ NM +EAGG NG I P+  T  Y+
Sbjct:  131 ALGAFATGVGSTDLAAGIALGEAWFKVPEAIKFEYEGKLPEDVTGKDLILHTIGDIGVDGALYQSMEFTGSAIDDLSMDGRMTMCNMAIEAGGKNGIIAPDKKTIAYV 238

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BLAST of FL512446 vs. TrEMBL
Match: B9SQS5_RICCO (3-isopropylmalate dehydratase, putative OS=Ricinus communis GN=RCOM_1217310 PE=4 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 3.426e-47
Identity = 96/108 (88.89%), Postives = 101/108 (93.52%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            AFGQFATGIGNTDAGFVLG GKLLLKVPPTLRFVMDGEMP+YLLAKDLILQIIGEISV+GA YKSMEFVG+TVESLNMEERMTL NMVVEAGG NG IP +S T+KYL
Sbjct:  208 AFGQFATGIGNTDAGFVLGTGKLLLKVPPTLRFVMDGEMPDYLLAKDLILQIIGEISVSGATYKSMEFVGTTVESLNMEERMTLCNMVVEAGGKNGVIPSDSTTFKYL 315

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BLAST of FL512446 vs. TrEMBL
Match: B9HKV5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_820830 PE=4 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 9.969e-47
Identity = 93/108 (86.11%), Postives = 100/108 (92.59%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            AFGQFATGIGNTDAGFVLG GKLLLKVPPTL+FVMDGEMP+YLLAKDLILQIIGEISVAGA YKSMEF G+TVESL MEERMTL NMV+EAGG NG +PP+S T+KYL
Sbjct:  141 AFGQFATGIGNTDAGFVLGTGKLLLKVPPTLKFVMDGEMPDYLLAKDLILQIIGEISVAGATYKSMEFAGTTVESLTMEERMTLCNMVIEAGGKNGVVPPDSTTFKYL 248

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BLAST of FL512446 vs. TrEMBL
Match: D7TL65_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_56.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00029978001 PE=4 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 2.221e-46
Identity = 94/108 (87.04%), Postives = 100/108 (92.59%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            AFGQFATGIGNTDAGFVLG GK+LLKVPPTLRFVMDGEMP+YLLAKDLILQIIGEISV+GA YKSMEFVGSTVESL MEERMTL NMVVEAGG NG +P +S T+KYL
Sbjct:  206 AFGQFATGIGNTDAGFVLGTGKILLKVPPTLRFVMDGEMPDYLLAKDLILQIIGEISVSGATYKSMEFVGSTVESLTMEERMTLCNMVVEAGGKNGVVPADSTTHKYL 313

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BLAST of FL512446 vs. TrEMBL
Match: B9HSU8_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_821804 PE=4 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 2.221e-46
Identity = 95/108 (87.96%), Postives = 99/108 (91.67%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            AFGQFATGIGNTDAGFVLG GKLLLKVPPTLRFVMDGEMP+YL AKDLILQIIGEISVAGA YKSMEFVGSTVESL MEERMTL NMVVEAGG NG +P +S T+KYL
Sbjct:  160 AFGQFATGIGNTDAGFVLGTGKLLLKVPPTLRFVMDGEMPDYLQAKDLILQIIGEISVAGATYKSMEFVGSTVESLTMEERMTLCNMVVEAGGKNGVVPADSTTFKYL 267

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BLAST of FL512446 vs. TrEMBL
Match: D7SZP6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_49.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00027922001 PE=4 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 8.439e-46
Identity = 92/108 (85.19%), Postives = 99/108 (91.67%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            AFGQFATGIGNTDA FVLG GK+LLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISV+GA YKSMEFVG+TVESL MEERMTL NMV+EAGG NG +P +S T+KYL
Sbjct:  206 AFGQFATGIGNTDAAFVLGTGKILLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVSGATYKSMEFVGTTVESLTMEERMTLCNMVIEAGGKNGVVPADSTTHKYL 313

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BLAST of FL512446 vs. TrEMBL
Match: C5XT62_SORBI (Putative uncharacterized protein Sb04g002120 OS=Sorghum bicolor GN=Sb04g002120 PE=4 SV=1)

HSP 1 Score: 184.882 bits (468), Expect = 2.455e-45
Identity = 91/111 (81.98%), Postives = 99/111 (89.19%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYLAGQ 336
            AFGQFATGIGNTDAGFVLG GK LLKVPPT+RFV+DGEMP YLLAKDLILQIIGEISV+GA YKSMEFVGSTVESL MEERMTL NMV+EAGG NG +P +  T+KYL G+
Sbjct:  205 AFGQFATGIGNTDAGFVLGTGKALLKVPPTIRFVLDGEMPPYLLAKDLILQIIGEISVSGATYKSMEFVGSTVESLTMEERMTLCNMVIEAGGKNGVVPADETTFKYLEGK 315

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BLAST of FL512446 vs. TrEMBL
Match: Q6Z702_ORYSJ (Os02g0125100 protein OS=Oryza sativa subsp. japonica GN=P0575F10.36 PE=2 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 3.207e-45
Identity = 90/111 (81.08%), Postives = 99/111 (89.19%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYLAGQ 336
            AFGQFATGIGNTDAGFV+G GK LLKVPPT+RFV+DGEMP YLLAKDLILQIIGEISV+GA YKSMEFVGSTVESLNMEERMTL NMV+EAGG NG +P +  T+ YL G+
Sbjct:  212 AFGQFATGIGNTDAGFVMGTGKALLKVPPTIRFVLDGEMPPYLLAKDLILQIIGEISVSGATYKSMEFVGSTVESLNMEERMTLCNMVIEAGGKNGVVPADQTTFNYLEGK 322

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BLAST of FL512446 vs. TrEMBL
Match: C4JBG7_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 3.207e-45
Identity = 91/111 (81.98%), Postives = 99/111 (89.19%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYLAGQ 336
            AFGQFATGIGNTDAGFV+G GK LLKVPPT+RFV+DGEMP YLLAKDLILQIIGEISV+GA YKSMEFVGSTVESL MEERMTL NMVVEAGG NG +P +  T+KYL G+
Sbjct:  203 AFGQFATGIGNTDAGFVMGTGKALLKVPPTIRFVLDGEMPPYLLAKDLILQIIGEISVSGATYKSMEFVGSTVESLTMEERMTLCNMVVEAGGKNGVVPADETTFKYLEGR 313

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BLAST of FL512446 vs. TrEMBL
Match: C4IZQ5_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 3.207e-45
Identity = 91/111 (81.98%), Postives = 99/111 (89.19%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYLAGQ 336
            AFGQFATGIGNTDAGFV+G GK LLKVPPT+RFV+DGEMP YLLAKDLILQIIGEISV+GA YKSMEFVGSTVESL MEERMTL NMVVEAGG NG +P +  T+KYL G+
Sbjct:  203 AFGQFATGIGNTDAGFVMGTGKALLKVPPTIRFVLDGEMPPYLLAKDLILQIIGEISVSGATYKSMEFVGSTVESLTMEERMTLCNMVVEAGGKNGVVPADETTFKYLEGR 313

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BLAST of FL512446 vs. TrEMBL
Match: B8AGY8_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_05669 PE=4 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 3.207e-45
Identity = 90/111 (81.08%), Postives = 99/111 (89.19%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYLAGQ 336
            AFGQFATGIGNTDAGFV+G GK LLKVPPT+RFV+DGEMP YLLAKDLILQIIGEISV+GA YKSMEFVGSTVESLNMEERMTL NMV+EAGG NG +P +  T+ YL G+
Sbjct:  212 AFGQFATGIGNTDAGFVMGTGKALLKVPPTIRFVLDGEMPPYLLAKDLILQIIGEISVSGATYKSMEFVGSTVESLNMEERMTLCNMVIEAGGKNGVVPADQTTFNYLEGK 322

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BLAST of FL512446 vs. TAIR peptide
Match: AT4G13430.1 (| Symbols: IIL1, ATLEUC1 | isopropyl malate isomerase large subunit 1 | chr4:7804194-7807789 REVERSE LENGTH=509)

HSP 1 Score: 178.333 bits (451), Expect = 2.010e-45
Identity = 86/108 (79.63%), Postives = 98/108 (90.74%), Query Frame = 1

Query:    4 AFGQFATGIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            AFGQFATGIGNTDAGFVLG GK+LLKVPPT+RF++DGEMP+YL AKDLILQIIGEISVAGA YK+MEF G+T+ESL+MEERMTL NMVVEAGG NG IPP++ T  Y+
Sbjct:  207 AFGQFATGIGNTDAGFVLGTGKILLKVPPTMRFILDGEMPSYLQAKDLILQIIGEISVAGATYKTMEFSGTTIESLSMEERMTLCNMVVEAGGKNGVIPPDATTLNYV 314

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BLAST of FL512446 vs. TAIR peptide
Match: AT2G05710.1 (| Symbols: ACO3 | aconitase 3 | chr2:2141591-2146350 FORWARD LENGTH=990)

HSP 1 Score: 56.225 bits (134), Expect = 1.152e-8
Identity = 29/101 (28.71%), Postives = 53/101 (52.48%), Query Frame = 1

Query:   25 GIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            G+G  +A   +    + + +P  + F + G+M N + A DL+L +   +   G + K +EF G+ +  L++ +R T+ NM  E G   G  P + +T +YL
Sbjct:  316 GVGGIEAEATMLGQPMSMVLPGVVGFKLAGKMRNGVTATDLVLTVTQMLRKHGVVGKFVEFYGNGMSGLSLADRATIANMSPEYGATMGFFPVDHVTLQYL 416

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BLAST of FL512446 vs. TAIR peptide
Match: AT4G26970.1 (| Symbols: ACO2 | aconitase 2 | chr4:13543077-13548427 FORWARD LENGTH=995)

HSP 1 Score: 55.4546 bits (132), Expect = 1.965e-8
Identity = 28/101 (27.72%), Postives = 52/101 (51.49%), Query Frame = 1

Query:   25 GIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            G+G  +A   +    + + +P  + F +DG++   + A DL+L +   +   G + K +EF G  +  L++ +R T+ NM  E G   G  P + +T +YL
Sbjct:  321 GVGGIEAEAAMLGQPMSMVLPGVVGFKLDGKLKEGVTATDLVLTVTQILRKHGVVGKFVEFYGEGMSELSLADRATIANMSPEYGATMGFFPVDHVTLEYL 421

[Close]

BLAST of FL512446 vs. TAIR peptide
Match: AT4G35830.2 (| Symbols: ACO1 | aconitase 1 | chr4:16973007-16977278 REVERSE LENGTH=795)

HSP 1 Score: 52.7582 bits (125), Expect = 1.273e-7
Identity = 27/101 (26.73%), Postives = 52/101 (51.49%), Query Frame = 1

Query:   25 GIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            G+G  +A   +    + + +P  + F + G++ + + A DL+L +   +   G + K +EF G  +  L++ +R T+ NM  E G   G  P + +T +YL
Sbjct:  121 GVGGIEAEATMLGQPMSMVLPGVVGFKLTGKLRDGMTATDLVLTVTQMLRKHGVVGKFVEFHGEGMRELSLADRATIANMSPEYGATMGFFPVDHVTLQYL 221

[Close]

BLAST of FL512446 vs. TAIR peptide
Match: AT4G35830.1 (| Symbols: ACO1 | aconitase 1 | chr4:16973007-16977949 REVERSE LENGTH=898)

HSP 1 Score: 52.7582 bits (125), Expect = 1.273e-7
Identity = 27/101 (26.73%), Postives = 52/101 (51.49%), Query Frame = 1

Query:   25 GIGNTDAGFVLGAGKLLLKVPPTLRFVMDGEMPNYLLAKDLILQIIGEISVAGAIYKSMEFVGSTVESLNMEERMTLGNMVVEAGGPNGAIPPESITYKYL 327
            G+G  +A   +    + + +P  + F + G++ + + A DL+L +   +   G + K +EF G  +  L++ +R T+ NM  E G   G  P + +T +YL
Sbjct:  224 GVGGIEAEATMLGQPMSMVLPGVVGFKLTGKLRDGMTATDLVLTVTQMLRKHGVVGKFVEFHGEGMRELSLADRATIANMSPEYGATMGFFPVDHVTLQYL 324

The following BLAST results are available for this feature:

BLAST of FL512446 vs. SwissProt
BLAST of FL512446 vs. TrEMBL
BLAST of FL512446 vs. TAIR peptide

BLAST of FL512446 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
LEUC_ARATH	1.808e-44	79.63	3-isopropylmalate dehydratase OS=Arabidopsis thali...	[more]
LEUC_HALOH	8.437e-26	50.00	3-isopropylmalate dehydratase large subunit OS=Hal...	[more]
LEUC_SYNAS	9.328e-25	49.07	3-isopropylmalate dehydratase large subunit OS=Syn...	[more]
LEUC_WOLSU	1.218e-24	47.75	3-isopropylmalate dehydratase large subunit OS=Wol...	[more]
LEUC1_PYRAB	1.218e-24	47.22	3-isopropylmalate dehydratase large subunit 1 OS=P...	[more]
LEUC_AQUAE	1.591e-24	44.44	3-isopropylmalate dehydratase large subunit OS=Aqu...	[more]
LEUC1_PYRFU	4.629e-24	45.05	3-isopropylmalate dehydratase large subunit 1 OS=P...	[more]
LEUC_CLOPH	6.046e-24	46.30	3-isopropylmalate dehydratase large subunit OS=Clo...	[more]
LEUC_METJA	1.347e-23	48.15	Isopropylmalate/citramalate isomerase large subuni...	[more]
LEUC_HELMI	1.347e-23	47.22	3-isopropylmalate dehydratase large subunit OS=Hel...	[more]

BLAST of FL512446 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SQS5_RICCO	3.426e-47	88.89	3-isopropylmalate dehydratase, putative OS=Ricinus...	[more]
B9HKV5_POPTR	9.969e-47	86.11	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7TL65_VITVI	2.221e-46	87.04	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9HSU8_POPTR	2.221e-46	87.96	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7SZP6_VITVI	8.439e-46	85.19	Whole genome shotgun sequence of line PN40024, sca...	[more]
C5XT62_SORBI	2.455e-45	81.98	Putative uncharacterized protein Sb04g002120 OS=So...	[more]
Q6Z702_ORYSJ	3.207e-45	81.08	Os02g0125100 protein OS=Oryza sativa subsp. japoni...	[more]
C4JBG7_MAIZE	3.207e-45	81.98	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
C4IZQ5_MAIZE	3.207e-45	81.98	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B8AGY8_ORYSI	3.207e-45	81.08	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of FL512446 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT4G13430.1	2.010e-45	79.63	\| Symbols: IIL1, ATLEUC1 \| isopropyl malate isomer...	[more]
AT2G05710.1	1.152e-8	28.71	\| Symbols: ACO3 \| aconitase 3 \| chr2:2141591-21463...	[more]
AT4G26970.1	1.965e-8	27.72	\| Symbols: ACO2 \| aconitase 2 \| chr4:13543077-1354...	[more]
AT4G35830.2	1.273e-7	26.73	\| Symbols: ACO1 \| aconitase 1 \| chr4:16973007-1697...	[more]
AT4G35830.1	1.273e-7	26.73	\| Symbols: ACO1 \| aconitase 1 \| chr4:16973007-1697...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001030	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha	PFAM	PF00330	Aconitase	coord: 2..113 score: 3.3
IPR001030	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha	SUPERFAMILY	53732	Aconitase iron-sulfur domain	coord: 2..113 score: 8.2
IPR015932	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 2	GENE3D	3.40.1060.10	no description	coord: 33..113 score: 5.6
IPR015936	Homoaconitase/3-isopropylmalate dehydratase, small/large subunit	PANTHER	PTHR11670:SF6	3-ISOPROPYLMALATE DEHYDRATASE	coord: 2..115 score: 2.9
IPR015937	Aconitase/isopropylmalate dehydratase	PANTHER	PTHR11670	ACONITASE	coord: 2..115 score: 2.9

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FL512446 ID=FL512446; Name=FL512446; organism=Cicer arietinum; type=EST; length=513bp
GCTGCATTTGGTCAATTTGCAACAGGAATCGGAAATACTGATGCCGGTTT
TGTGTTGGGAGCAGGGAAGCTTTTGCTAAAGGTGCCTCCAACTTTGAGAT
TTGTAATGGATGGGGAAATGCCCAATTATCTGCTTGCAAAGGATTTGATT
CTGCAAATTATCGGTGAAATATCCGTGGCTGGGGCGATTTACAAATCTAT
GGAGTTTGTTGGTTCAACTGTTGAAAGTTTAAATATGGAAGAACGGATGA
CATTGGGCAACATGGTTGTTGAAGCTGGAGGACCAAATGGCGCTATTCCT
CCTGAAAGCATTACATATAAATATCTCGCAGGGCAAAACATCCGGTGCCA
TCATGAAACCAGAATTATAGTGCCCGGCCCAAGCCAATATCTCCTTCTCT
GAAAGATCAAACTCTTGATCGCCTCACACAAATCGTACCCCCTGGGTTTG
CCGAAAACCTCTTTCCACCAATAACACCCGGTGTTTTTCGCCGAAGACAA
GGGCACCGGAATG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process
GO:0008652	amino acid biosynthetic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016836	hydro-lyase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR001030	Acoase/IPM_deHydtase_lsu_aba
IPR015932	Aconitase_dom2
IPR015936	Homoaconitase/3-isopropylmalate dehydratase, small/large subunit
IPR015937	Acoase/IPM_deHydtase

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