FL512447

EST Overview


Name	FL512447
Unique Name	FL512447
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	515

Libraries


Library Name	Type
LIBEST_023567 Subtracted cDNA library of drought-stressed chickpea cultivar	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of FL512447 vs. SwissProt
Match: RFS_PEA (Galactinol--sucrose galactosyltransferase OS=Pisum sativum GN=RFS PE=1 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 1.252e-45
Identity = 85/147 (57.82%), Postives = 116/147 (78.91%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKP 442
            GVLG+FNCQGGGW  + R NK ASEFS  V+   + +DIEW  GK+P+ I+GV +FA+YF + KKL L    D  E+SLEPF+FEL+T+SP+ +F K+  +FAPIGLVNMLN+GGA+QS +FD++ +LV++GVRG GE++V+AS+KP
Sbjct:  614 GVLGLFNCQGGGWCPETRRNKSASEFSHAVTCYASPEDIEWCNGKTPMDIKGVDVFAVYFFKEKKLSLMKCSDRLEVSLEPFSFELMTVSPLKVFSKRLIQFAPIGLVNMLNSGGAVQSLEFDDSASLVKIGVRGCGELSVFASEKP 760

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BLAST of FL512447 vs. SwissProt
Match: RFS5_ARATH (Probable galactinol--sucrose galactosyltransferase 5 OS=Arabidopsis thaliana GN=RFS5 PE=1 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 5.095e-39
Identity = 81/147 (55.10%), Postives = 103/147 (70.07%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKP 442
            GV+G FNCQGGGW R+ R N+C SE    ++   + KD+EW+ G SPISI  V+ FAL+ +Q+KKL L   +D  E++LEPF FELIT+SPV      S +FAPIGLVNMLN  GAI+S  +++    VEVGV G+GE  VYASKKP
Sbjct:  601 GVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGSSPISIANVEEFALFLSQSKKLLLSGLNDDLELTLEPFKFELITVSPVVTIEGNSVRFAPIGLVNMLNTSGAIRSLVYNDES--VEVGVFGAGEFRVYASKKP 745

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BLAST of FL512447 vs. SwissProt
Match: RFS_ORYSJ (Galactinol--sucrose galactosyltransferase OS=Oryza sativa subsp. japonica GN=RFS PE=1 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 1.486e-30
Identity = 69/151 (45.70%), Postives = 96/151 (63.58%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTEEISLEPFNFELITISPV--TLFPKKSFKFAPIGLVNMLNNGGAIQSFDF--DEAQNLVEVGVRGSGEMTVYASKKP 442
            GVLG FNCQGGGW R+ R N CA+ FS  V+ + +  D+EWS G       G   FA+YF +A+KL+L    ++ E++LEPF +EL+ ++PV   + P+    FAPIGL NMLN GGA+Q F+    +     EV V+G+GEM  Y+S +P
Sbjct:  599 GVLGAFNCQGGGWSREARRNMCAAGFSVPVTARASPADVEWSHGGG-----GGDRFAVYFVEARKLQLLRRDESVELTLEPFTYELLVVAPVRAIVSPELGIGFAPIGLANMLNAGGAVQGFEAARKDGDVAAEVAVKGAGEMVAYSSARP 744

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BLAST of FL512447 vs. SwissProt
Match: STSYN_PEA (Stachyose synthase OS=Pisum sativum GN=STS1 PE=1 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 3.213e-17
Identity = 47/150 (31.33%), Postives = 80/150 (53.33%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFS-PHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKPTK 448
            GV+G FNCQG GW   ++  +   E  + +   +++ ++EW   +    +   + + +Y NQA++L L +   +  + +++P  FEL +  PVT       KFAPIGL NM N+GG +   D +   N  ++ V+G G    Y+S+ P K
Sbjct:  669 GVIGAFNCQGAGWDPIMQKFRGFPECYKPIPGTVHVTEVEWDQKEETSHLGKAEEYVVYLNQAEELSLMTLKSEPIQFTIQPSTFELYSFVPVTKL-CGGIKFAPIGLTNMFNSGGTV--IDLEYVGNGAKIKVKGGGSFLAYSSESPKK 815

[Close]

BLAST of FL512447 vs. SwissProt
Match: RFS4_ARATH (Probable galactinol--sucrose galactosyltransferase 4 OS=Arabidopsis thaliana GN=RFS4 PE=2 SV=2)

HSP 1 Score: 84.7297 bits (208), Expect = 2.720e-16
Identity = 51/154 (33.12%), Postives = 76/154 (49.35%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWS----IGKSPISIEGVKLFALYFNQAKKLKLF-SPHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKPTK 448
            GV+G FNCQG GW  +    K   E    VS  +++ DIEW        S ++  G  L  +Y  Q++++    S  +  +I+LEP  F+L++  PVT       +F P+GL+NM N  G +Q        N + V V+G G    Y+S  P K
Sbjct:  687 GVIGTFNCQGAGWSPEEHRFKGYKECYTTVSGTVHVSDIEWDQNPEAAGSQVTYTGDYL--VYKQQSEEILFMNSKSEAMKITLEPSAFDLLSFVPVTELVSSGVRFVPLGLINMFNCVGTVQDMKV-TGDNSIRVDVKGEGRFMAYSSSAPVK 837

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BLAST of FL512447 vs. SwissProt
Match: RFS1_ARATH (Probable galactinol--sucrose galactosyltransferase 1 OS=Arabidopsis thaliana GN=RFS1 PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 2.155e-13
Identity = 47/147 (31.97%), Postives = 80/147 (54.42%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTE-EISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDE--AQNLVEVGVRGSGEMTVYAS 433
            GV+GVFNCQG GW ++ +      +    +S  +   D+ +    +     G    ++ ++  +   ++ P DT   ++L P  +E+ T+ PV  F   S KFAP+GL+ M N+GGAI S  +D+   + +V + +RGSG + VY+S
Sbjct:  568 GVIGVFNCQGAGWCKNEKRYLIHDQEPGTISGCVRTNDVHYLHKVAAFEWTGD---SIVYSHLRGELVYLPKDTSLPVTLMPREYEVFTVVPVKEFSDGS-KFAPVGLMEMFNSGGAIVSLRYDDEGTKFVVRMKLRGSGLVGVYSS 710

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BLAST of FL512447 vs. SwissProt
Match: RFS6_ARATH (Probable galactinol--sucrose galactosyltransferase 6 OS=Arabidopsis thaliana GN=RFS6 PE=2 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 5.309e-12
Identity = 46/150 (30.67%), Postives = 77/150 (51.33%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTE-EISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKPTK 448
            GVLGV+NCQG  W    R N      +  ++  I  +D+  SI ++             ++Q++   +  P++    +SL+    E+ T+SP++        FAPIGLVNM N+GGAI+   ++  +  V + V+G G+   Y+S KP +
Sbjct:  562 GVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVH-SISEASTDPTTWNGDCAVYSQSRGELIVMPYNVSLPVSLKIREHEIFTVSPISHLVD-GVSFAPIGLVNMYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKR 709

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BLAST of FL512447 vs. SwissProt
Match: RFS2_ARATH (Probable galactinol--sucrose galactosyltransferase 2 OS=Arabidopsis thaliana GN=RFS2 PE=2 SV=2)

HSP 1 Score: 65.4698 bits (158), Expect = 1.708e-10
Identity = 46/174 (26.44%), Postives = 78/174 (44.83%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQN-------------------------LVEVGVRGSGEMTVYASKKPTK 448
            G++GVFNCQG GW ++ + N+        ++  I   D +     +     G  +  +Y  ++ ++       +  ++L+   +EL  ISP+     ++  FAPIGLV+M N+ GAI+S D +   +                         LV V VRG G    Y+S++P K
Sbjct:  564 GIVGVFNCQGAGWCKETKKNQIHDTSPGTLTGSIRADDADLISQVAGEDWSGDSI--VYAYRSGEVVRLPKGASIPLTLKVLEYELFHISPLKEI-TENISFAPIGLVDMFNSSGAIESIDINHVTDKNPEFFDGEISSASPALSDNRSPTALVSVSVRGCGRFGAYSSQRPLK 734

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BLAST of FL512447 vs. TrEMBL
Match: B2ZF65_SOYBN (Raffionse synthase 3 OS=Glycine max GN=RS3 PE=4 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 9.288e-61
Identity = 117/148 (79.05%), Postives = 130/148 (87.84%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHD-TEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKP 442
            GVLGVFNCQGGGWFR+IRSNKCA+EFS  VSTK NIKDIEW  GK+PISIEGV+LFA YF+QAKKL L +P D +EEISLEPFNFELIT+SPVT+ P KS KFAPIGLVNMLN GGA+QS  FDE QNLVEVG+RG+GEM VYAS+KP
Sbjct:  573 GVLGVFNCQGGGWFREIRSNKCAAEFSHRVSTKTNIKDIEWDSGKNPISIEGVQLFASYFSQAKKLILSAPSDDSEEISLEPFNFELITVSPVTVLPGKSVKFAPIGLVNMLNTGGAVQSLAFDEGQNLVEVGLRGTGEMRVYASEKP 720

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BLAST of FL512447 vs. TrEMBL
Match: D2DW73_PHAVU (Putative raffinose synthase protein OS=Phaseolus vulgaris PE=4 SV=1)

HSP 1 Score: 231.876 bits (590), Expect = 1.752e-59
Identity = 113/147 (76.87%), Postives = 127/147 (86.39%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKP 442
            GVLGVFNCQGGGWFR+ RSNKCA+EFS  VSTK N KDIEW  G +PISIEGV+LFALYF+Q+KKL L +P D+EEISLEPFNFELIT+SPVT+   KS KFAPIGLVNMLN G A+QS  FDEAQNLVEVGVRG+GEM VYAS++P
Sbjct:  580 GVLGVFNCQGGGWFRETRSNKCAAEFSHKVSTKTNPKDIEWDSGNNPISIEGVELFALYFSQSKKLVLSAPSDSEEISLEPFNFELITVSPVTVLSGKSVKFAPIGLVNMLNTGAALQSLTFDEAQNLVEVGVRGTGEMRVYASERP 726

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BLAST of FL512447 vs. TrEMBL
Match: B9MZ18_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_595025 PE=4 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 4.324e-50
Identity = 94/147 (63.95%), Postives = 119/147 (80.95%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKP 442
            GV+G FNCQGGGW R+ R NKCAS+FS LV+ K N  DIEW+ GK+PISIEGV++FA+YF+Q+K L L  P+D  E++LEPFNFELIT+SPVT    KS +FAPIGLVNMLN GGAIQS  +++A + V++GV G+GEM V+AS+KP
Sbjct:  591 GVIGAFNCQGGGWCRETRRNKCASQFSHLVTAKTNPNDIEWNSGKNPISIEGVQVFAMYFSQSKMLVLCKPYDNIEMALEPFNFELITVSPVTALAGKSVQFAPIGLVNMLNTGGAIQSLAYNDANSSVQIGVTGTGEMRVFASEKP 737

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BLAST of FL512447 vs. TrEMBL
Match: B9SF88_RICCO (Stachyose synthase, putative OS=Ricinus communis GN=RCOM_1095590 PE=4 SV=1)

HSP 1 Score: 193.356 bits (490), Expect = 6.904e-48
Identity = 93/147 (63.27%), Postives = 116/147 (78.91%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKP 442
            GV+GVFNCQGGGW R+ R NKCAS+FS LV+ K N KDIEW  G +P SIEGV++FA+Y  +AKKL L  P++  EI+LEPFNFELIT+SPV    +KS +FAPIGLVNMLN GGA+QS  ++ A + +E+GVRG GEM V+AS+KP
Sbjct:  602 GVIGVFNCQGGGWCRETRRNKCASQFSHLVTAKTNAKDIEWKNGTNPNSIEGVQVFAMYLFKAKKLLLSKPYENIEIALEPFNFELITVSPVVTLSEKSIQFAPIGLVNMLNTGGAMQSLSYN-ADSSIEIGVRGEGEMRVFASEKP 747

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BLAST of FL512447 vs. TrEMBL
Match: B9MXY7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_594470 PE=4 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 9.969e-47
Identity = 89/148 (60.14%), Postives = 118/148 (79.73%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDF-DEAQNLVEVGVRGSGEMTVYASKKP 442
            GV+G FNCQGGGW R+ R NKCA++FS  V+ K N +DIEW+ GK+PISIEGV++FA+Y +++KKL L   H+  EI+LEPFNFELIT+SPVT    K  +FAPIGLVNMLN GGAIQS  + +++ + V++G++GSGEM V+AS+KP
Sbjct:  598 GVIGTFNCQGGGWCRETRRNKCAAQFSHSVTAKTNPRDIEWNSGKNPISIEGVQIFAMYLSKSKKLVLSKAHENIEIALEPFNFELITVSPVTTLAGKPAQFAPIGLVNMLNTGGAIQSLAYTNDSNSSVQIGIKGSGEMRVFASEKP 745

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BLAST of FL512447 vs. TrEMBL
Match: Q9ZT62_CUCSA (Raffinose synthase OS=Cucumis sativus GN=Rfs PE=2 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 3.788e-46
Identity = 91/147 (61.90%), Postives = 112/147 (76.19%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKP 442
            GV+G FNCQGGGW R+ R N+C S++S+ V++K N KDIEW  G++PISIEGVK FALY  QAKKL L  P    +I+L+PF FELIT+SPVT   + S  FAPIGLVNMLN  GAIQS D+D+  + VE+GV+G GEM V+ASKKP
Sbjct:  596 GVIGAFNCQGGGWCRETRRNQCFSQYSKRVTSKTNPKDIEWHSGENPISIEGVKTFALYLYQAKKLILSKPSQDLDIALDPFEFELITVSPVTKLIQTSLHFAPIGLVNMLNTSGAIQSVDYDDDLSSVEIGVKGCGEMRVFASKKP 742

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BLAST of FL512447 vs. TrEMBL
Match: D7TWK5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_66.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00032425001 PE=4 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 3.788e-46
Identity = 92/149 (61.74%), Postives = 114/149 (76.51%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKS--PISIEGVKLFALYFNQAKKLKLFSPHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKP 442
            GVLG FNCQGGGW R+ R NKCAS+FS  V++  + KDIEW  G S  PISIEGV+LFA+Y  + KKL L  P    EISL+PF+FELIT+SPVT  P KS +FAPIGLVNMLN+GGAI+S  FD+ +N V +GV+G+GEM  +A++KP
Sbjct:  589 GVLGAFNCQGGGWCREARRNKCASQFSHAVTSVASPKDIEWRNGNSSTPISIEGVQLFAMYMFRTKKLVLSKPSQNIEISLDPFDFELITVSPVTTLPGKSVQFAPIGLVNMLNSGGAIESLAFDDEENSVRIGVKGTGEMRAFAAEKP 737

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BLAST of FL512447 vs. TrEMBL
Match: B5G4T9_CUCSA (Raffinose synthase OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 3.788e-46
Identity = 91/147 (61.90%), Postives = 112/147 (76.19%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKP 442
            GV+G FNCQGGGW R+ R N+C S++S+ V++K N KDIEW  G++PISIEGVK FALY  QAKKL L  P    +I+L+PF FELIT+SPVT   + S  FAPIGLVNMLN  GAIQS D+D+  + VE+GV+G GEM V+ASKKP
Sbjct:  596 GVIGAFNCQGGGWCRETRRNQCFSQYSKRVTSKTNPKDIEWHSGENPISIEGVKTFALYLYQAKKLILSKPSQDLDIALDPFEFELITVSPVTKLIQTSLHFAPIGLVNMLNTSGAIQSVDYDDDLSSVEIGVKGCGEMRVFASKKP 742

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BLAST of FL512447 vs. TrEMBL
Match: A5AN06_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021070 PE=4 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 3.788e-46
Identity = 92/149 (61.74%), Postives = 114/149 (76.51%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKS--PISIEGVKLFALYFNQAKKLKLFSPHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKP 442
            GVLG FNCQGGGW R+ R NKCAS+FS  V++  + KDIEW+ G S  PISIEGV+LFA+Y  + KKL L  P    EISL+PF+FELIT+SPVT  P KS +FAPIGLVNMLN+GGAI+S  FD+ +N V +GV+G+GEM  +A +KP
Sbjct:  576 GVLGAFNCQGGGWCREARRNKCASQFSHAVTSVASPKDIEWTNGNSSTPISIEGVQLFAMYMFRTKKLVLSKPSQNIEISLDPFDFELITVSPVTTLPGKSVQFAPIGLVNMLNSGGAIESLAFDDEENSVRIGVKGTGEMRAFAXEKP 724

[Close]

BLAST of FL512447 vs. TrEMBL
Match: B9IEW5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_776137 PE=4 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 3.002e-43
Identity = 84/147 (57.14%), Postives = 113/147 (76.87%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKP 442
            GVLG+FNCQGGGW    R NK A++FS+ V+   + KDIEW+ GKSPIS++GV +FA+Y  + KK++L    +  EISLEPFN++L+T+SPVT+ P+KS +FAPIGLVNMLN GGAIQS    + ++L+ +GV+GSGEM V+AS  P
Sbjct:  581 GVLGIFNCQGGGWCPVARRNKSANQFSQSVTCSASPKDIEWNSGKSPISVKGVDVFAVYMFKEKKVRLLKSSEKLEISLEPFNYDLLTVSPVTVLPRKSIQFAPIGLVNMLNTGGAIQSVMVVDDESLIRIGVKGSGEMRVFASGNP 727

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BLAST of FL512447 vs. TAIR peptide
Match: AT5G40390.1 (| Symbols: SIP1 | Raffinose synthase family protein | chr5:16161720-16165085 FORWARD LENGTH=783)

HSP 1 Score: 160.229 bits (404), Expect = 5.663e-40
Identity = 81/147 (55.10%), Postives = 103/147 (70.07%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKP 442
            GV+G FNCQGGGW R+ R N+C SE    ++   + KD+EW+ G SPISI  V+ FAL+ +Q+KKL L   +D  E++LEPF FELIT+SPV      S +FAPIGLVNMLN  GAI+S  +++    VEVGV G+GE  VYASKKP
Sbjct:  601 GVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGSSPISIANVEEFALFLSQSKKLLLSGLNDDLELTLEPFKFELITVSPVVTIEGNSVRFAPIGLVNMLNTSGAIRSLVYNDES--VEVGVFGAGEFRVYASKKP 745

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BLAST of FL512447 vs. TAIR peptide
Match: AT4G01970.1 (| Symbols: AtSTS, STS | stachyose synthase | chr4:854073-856953 REVERSE LENGTH=876)

HSP 1 Score: 86.2705 bits (212), Expect = 1.039e-17
Identity = 52/154 (33.77%), Postives = 77/154 (50.00%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWS----IGKSPISIEGVKLFALYFNQAKKLKLF-SPHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKPTK 448
            GV+G FNCQG GW  +    K   E    VS  +++ DIEW        S ++  G  L  +Y  Q++++    S  +  +I+LEP  F+L++  PVT       +FAP+GL+NM N  G +Q        N + V V+G G    Y+S  P K
Sbjct:  687 GVIGTFNCQGAGWSPEEHRFKGYKECYTTVSGTVHVSDIEWDQNPEAAGSQVTYTGDYL--VYKQQSEEILFMNSKSEAMKITLEPSAFDLLSFVPVTELVSSGVRFAPLGLINMFNCVGTVQDMKV-TGDNSIRVDVKGEGRFMAYSSSAPVK 837

[Close]

BLAST of FL512447 vs. TAIR peptide
Match: AT1G55740.1 (| Symbols: AtSIP1, SIP1 | seed imbibition 1 | chr1:20835507-20838707 REVERSE LENGTH=754)

HSP 1 Score: 75.0998 bits (183), Expect = 2.396e-14
Identity = 47/147 (31.97%), Postives = 80/147 (54.42%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTE-EISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDE--AQNLVEVGVRGSGEMTVYAS 433
            GV+GVFNCQG GW ++ +      +    +S  +   D+ +    +     G    ++ ++  +   ++ P DT   ++L P  +E+ T+ PV  F   S KFAP+GL+ M N+GGAI S  +D+   + +V + +RGSG + VY+S
Sbjct:  568 GVIGVFNCQGAGWCKNEKRYLIHDQEPGTISGCVRTNDVHYLHKVAAFEWTGD---SIVYSHLRGELVYLPKDTSLPVTLMPREYEVFTVVPVKEFSDGS-KFAPVGLMEMFNSGGAIVSLRYDDEGTKFVVRMKLRGSGLVGVYSS 710

[Close]

BLAST of FL512447 vs. TAIR peptide
Match: AT5G20250.4 (| Symbols: DIN10 | Raffinose synthase family protein | chr5:6833730-6836635 FORWARD LENGTH=844)

HSP 1 Score: 70.4774 bits (171), Expect = 5.901e-13
Identity = 46/150 (30.67%), Postives = 77/150 (51.33%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTE-EISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKPTK 448
            GVLGV+NCQG  W    R N      +  ++  I  +D+  SI ++             ++Q++   +  P++    +SL+    E+ T+SP++        FAPIGLVNM N+GGAI+   ++  +  V + V+G G+   Y+S KP +
Sbjct:  657 GVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVH-SISEASTDPTTWNGDCAVYSQSRGELIVMPYNVSLPVSLKIREHEIFTVSPISHLVD-GVSFAPIGLVNMYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKR 804

[Close]

BLAST of FL512447 vs. TAIR peptide
Match: AT5G20250.3 (| Symbols: DIN10 | Raffinose synthase family protein | chr5:6834207-6836635 FORWARD LENGTH=749)

HSP 1 Score: 70.4774 bits (171), Expect = 5.901e-13
Identity = 46/150 (30.67%), Postives = 77/150 (51.33%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTE-EISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKPTK 448
            GVLGV+NCQG  W    R N      +  ++  I  +D+  SI ++             ++Q++   +  P++    +SL+    E+ T+SP++        FAPIGLVNM N+GGAI+   ++  +  V + V+G G+   Y+S KP +
Sbjct:  562 GVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVH-SISEASTDPTTWNGDCAVYSQSRGELIVMPYNVSLPVSLKIREHEIFTVSPISHLVD-GVSFAPIGLVNMYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKR 709

[Close]

BLAST of FL512447 vs. TAIR peptide
Match: AT5G20250.2 (| Symbols: DIN10 | Raffinose synthase family protein | chr5:6834207-6836635 FORWARD LENGTH=749)

HSP 1 Score: 70.4774 bits (171), Expect = 5.901e-13
Identity = 46/150 (30.67%), Postives = 77/150 (51.33%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTE-EISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKPTK 448
            GVLGV+NCQG  W    R N      +  ++  I  +D+  SI ++             ++Q++   +  P++    +SL+    E+ T+SP++        FAPIGLVNM N+GGAI+   ++  +  V + V+G G+   Y+S KP +
Sbjct:  562 GVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVH-SISEASTDPTTWNGDCAVYSQSRGELIVMPYNVSLPVSLKIREHEIFTVSPISHLVD-GVSFAPIGLVNMYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKR 709

[Close]

BLAST of FL512447 vs. TAIR peptide
Match: AT5G20250.1 (| Symbols: DIN10 | Raffinose synthase family protein | chr5:6834207-6836635 FORWARD LENGTH=749)

HSP 1 Score: 70.4774 bits (171), Expect = 5.901e-13
Identity = 46/150 (30.67%), Postives = 77/150 (51.33%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTE-EISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQNLVEVGVRGSGEMTVYASKKPTK 448
            GVLGV+NCQG  W    R N      +  ++  I  +D+  SI ++             ++Q++   +  P++    +SL+    E+ T+SP++        FAPIGLVNM N+GGAI+   ++  +  V + V+G G+   Y+S KP +
Sbjct:  562 GVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVH-SISEASTDPTTWNGDCAVYSQSRGELIVMPYNVSLPVSLKIREHEIFTVSPISHLVD-GVSFAPIGLVNMYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKR 709

[Close]

BLAST of FL512447 vs. TAIR peptide
Match: AT3G57520.1 (| Symbols: AtSIP2, SIP2 | seed imbibition 2 | chr3:21288982-21292694 REVERSE LENGTH=773)

HSP 1 Score: 65.4698 bits (158), Expect = 1.898e-11
Identity = 46/174 (26.44%), Postives = 78/174 (44.83%), Query Frame = 2

Query:    2 GVLGVFNCQGGGWFRDIRSNKCASEFSRLVSTKINIKDIEWSIGKSPISIEGVKLFALYFNQAKKLKLFSPHDTEEISLEPFNFELITISPVTLFPKKSFKFAPIGLVNMLNNGGAIQSFDFDEAQN-------------------------LVEVGVRGSGEMTVYASKKPTK 448
            G++GVFNCQG GW ++ + N+        ++  I   D +     +     G  +  +Y  ++ ++       +  ++L+   +EL  ISP+     ++  FAPIGLV+M N+ GAI+S D +   +                         LV V VRG G    Y+S++P K
Sbjct:  564 GIVGVFNCQGAGWCKETKKNQIHDTSPGTLTGSIRADDADLISQVAGEDWSGDSI--VYAYRSGEVVRLPKGASIPLTLKVLEYELFHISPLKEI-TENISFAPIGLVDMFNSSGAIESIDINHVTDKNPEFFDGEISSASPALSDNRSPTALVSVSVRGCGRFGAYSSQRPLK 734

The following BLAST results are available for this feature:

BLAST of FL512447 vs. SwissProt
BLAST of FL512447 vs. TrEMBL
BLAST of FL512447 vs. TAIR peptide

BLAST of FL512447 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 8


Match Name	E-value	Identity	Description
RFS_PEA	1.252e-45	57.82	Galactinol--sucrose galactosyltransferase OS=Pisum...	[more]
RFS5_ARATH	5.095e-39	55.10	Probable galactinol--sucrose galactosyltransferase...	[more]
RFS_ORYSJ	1.486e-30	45.70	Galactinol--sucrose galactosyltransferase OS=Oryza...	[more]
STSYN_PEA	3.213e-17	31.33	Stachyose synthase OS=Pisum sativum GN=STS1 PE=1 S...	[more]
RFS4_ARATH	2.720e-16	33.12	Probable galactinol--sucrose galactosyltransferase...	[more]
RFS1_ARATH	2.155e-13	31.97	Probable galactinol--sucrose galactosyltransferase...	[more]
RFS6_ARATH	5.309e-12	30.67	Probable galactinol--sucrose galactosyltransferase...	[more]
RFS2_ARATH	1.708e-10	26.44	Probable galactinol--sucrose galactosyltransferase...	[more]

BLAST of FL512447 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B2ZF65_SOYBN	9.288e-61	79.05	Raffionse synthase 3 OS=Glycine max GN=RS3 PE=4 SV...	[more]
D2DW73_PHAVU	1.752e-59	76.87	Putative raffinose synthase protein OS=Phaseolus v...	[more]
B9MZ18_POPTR	4.324e-50	63.95	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SF88_RICCO	6.904e-48	63.27	Stachyose synthase, putative OS=Ricinus communis G...	[more]
B9MXY7_POPTR	9.969e-47	60.14	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q9ZT62_CUCSA	3.788e-46	61.90	Raffinose synthase OS=Cucumis sativus GN=Rfs PE=2 ...	[more]
D7TWK5_VITVI	3.788e-46	61.74	Whole genome shotgun sequence of line PN40024, sca...	[more]
B5G4T9_CUCSA	3.788e-46	61.90	Raffinose synthase OS=Cucumis sativus PE=2 SV=1	[more]
A5AN06_VITVI	3.788e-46	61.74	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9IEW5_POPTR	3.002e-43	57.14	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]

BLAST of FL512447 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 8


Match Name	E-value	Identity	Description
AT5G40390.1	5.663e-40	55.10	\| Symbols: SIP1 \| Raffinose synthase family protei...	[more]
AT4G01970.1	1.039e-17	33.77	\| Symbols: AtSTS, STS \| stachyose synthase \| chr4:...	[more]
AT1G55740.1	2.396e-14	31.97	\| Symbols: AtSIP1, SIP1 \| seed imbibition 1 \| chr1...	[more]
AT5G20250.4	5.901e-13	30.67	\| Symbols: DIN10 \| Raffinose synthase family prote...	[more]
AT5G20250.3	5.901e-13	30.67	\| Symbols: DIN10 \| Raffinose synthase family prote...	[more]
AT5G20250.2	5.901e-13	30.67	\| Symbols: DIN10 \| Raffinose synthase family prote...	[more]
AT5G20250.1	5.901e-13	30.67	\| Symbols: DIN10 \| Raffinose synthase family prote...	[more]
AT3G57520.1	1.898e-11	26.44	\| Symbols: AtSIP2, SIP2 \| seed imbibition 2 \| chr3...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008811	Raffinose synthase	PFAM	PF05691	Raffinose_syn	coord: 1..149 score: 3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FL512447 ID=FL512447; Name=FL512447; organism=Cicer arietinum; type=EST; length=515bp
TGGAGTTCTTGGTGTGTTTAACTGCCAAGGAGGAGGATGGTTTCGTGACA
TAAGATCAAACAAATGTGCTTCTGAGTTTTCTCGTTTGGTATCAACCAAA
ATCAACATCAAAGACATTGAATGGAGCATTGGAAAAAGTCCAATTTCAAT
TGAAGGTGTAAAACTTTTTGCTTTATATTTCAACCAAGCAAAAAAACTCA
AACTTTTCTCTCCACATGACACTGAAGAAATCTCTTTGGAACCATTTAAT
TTTGAGCTAATAACTATTTCACCTGTCACTTTATTTCCTAAAAAGTCCTT
CAAGTTTGCTCCTATTGGTTTGGTTAATATGTTAAACAATGGAGGGGCAA
TTCAGTCATTTGACTTTGATGAGGCTCAAAATTTGGTTGAAGTTGGAGTT
AGAGGTAGTGGGGAAATGACAGTGTATGCATCAAAGAAACCAACCAAATT
GTAGAATTGATGGGCATGGAAGTCGATTTTTAAATAAGAAAAGTTTATGG
TTGTAATTCAAGGTC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR008811	Glycosyl_hydrolases_36

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