GT618730

EST Overview


Name	GT618730
Unique Name	GT618730
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	697

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
KEGG analysis for Lens culinaris unigene v1	2011-01-05
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

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BLAST of GT618730 vs. TrEMBL
Match: B9T7U4_RICCO (Protein-s isoprenylcysteine O-methyltransferase, putative OS=Ricinus communis GN=RCOM_0408950 PE=4 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 3.156e-45
Identity = 84/129 (65.12%), Postives = 107/129 (82.95%), Query Frame = 3

Query:  309 MTEILSYTACRQLSQMFIAVIFFHVSEYFLAAFIHGKSSVTLKSLLISKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            MTEI SYTACRQLSQMF ++ FFH SEY LA  IHGKS+VTLKSLL+SKHYLLAM+FSL+EYL+E + FP LKEYW +SN         E+IRKMAI+TAG++FTH+I+++ +E H+L+THG+Y+F+RH
Sbjct:    1 MTEIFSYTACRQLSQMFFSISFFHGSEYLLAISIHGKSTVTLKSLLVSKHYLLAMLFSLVEYLIEFSLFPGLKEYWWVSNLGLVLVVIGEVIRKMAIITAGRAFTHLIKIYHEEHHKLITHGVYRFVRH 129

[Close]

BLAST of GT618730 vs. TrEMBL
Match: A9PB59_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 178.718 bits (452), Expect = 3.858e-43
Identity = 84/129 (65.12%), Postives = 103/129 (79.84%), Query Frame = 3

Query:  309 MTEILSYTACRQLSQMFIAVIFFHVSEYFLAAFIHGKSSVTLKSLLISKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            MTEI  YTACRQLSQMF+AV+FFH SEY L A IHG+SSV L SLLISK Y+ AM+F+LLEY+VE+  FP LKEYW +SN         EI RK+AIVTAGQSFTH+I+V+ +E+H L+THG+Y+F+RH
Sbjct:    1 MTEIFGYTACRQLSQMFLAVLFFHSSEYVLVAAIHGRSSVNLSSLLISKAYVFAMMFALLEYVVEIALFPGLKEYWWVSNLGLVMVIIGEITRKLAIVTAGQSFTHLIKVNHEEQHNLITHGVYRFVRH 129

[Close]

BLAST of GT618730 vs. TrEMBL
Match: B9HGE7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_802771 PE=4 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 1.915e-42
Identity = 83/129 (64.34%), Postives = 102/129 (79.07%), Query Frame = 3

Query:  309 MTEILSYTACRQLSQMFIAVIFFHVSEYFLAAFIHGKSSVTLKSLLISKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            MTEI  YTACRQL QMF+AV+FFH SEY L A IHG+SSV L SLLISK Y+ AM+F+LLEY+VE+  FP LKEYW +SN         EI RK+AIVTAGQSFTH+I+V+ +E+H L+THG+Y+F+RH
Sbjct:    1 MTEIFGYTACRQLFQMFLAVLFFHSSEYVLVAAIHGRSSVNLSSLLISKAYVFAMMFALLEYVVEIALFPGLKEYWWVSNLGLVMVIIGEITRKLAIVTAGQSFTHLIKVNHEEQHNLITHGVYRFVRH 129

[Close]

BLAST of GT618730 vs. TrEMBL
Match: B9H5P5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_801032 PE=4 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 1.241e-41
Identity = 83/129 (64.34%), Postives = 100/129 (77.52%), Query Frame = 3

Query:  309 MTEILSYTACRQLSQMFIAVIFFHVSEYFLAAFIHGKSSVTLKSLLISKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            MTEI  YTACRQLSQM +AV FFH SEY LAA IHG+S V+L SLLISK Y+ AM+FSLLEY+VE+  FP LKEYW +SN         EIIRK+AI+TAG +FTH+I+V+ +E H L+THGIY+F RH
Sbjct:    1 MTEIFGYTACRQLSQMILAVFFFHGSEYVLAAAIHGRSKVSLSSLLISKAYVFAMMFSLLEYVVEIALFPGLKEYWWVSNLGLVMVTIGEIIRKLAIITAGLAFTHLIKVYHEEHHNLITHGIYRFFRH 129

[Close]

BLAST of GT618730 vs. TrEMBL
Match: D7TR53_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_99.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00038782001 PE=4 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 3.611e-41
Identity = 87/135 (64.44%), Postives = 106/135 (78.52%), Query Frame = 3

Query:  294 LYLAKM-TEILSYTACRQLSQMFIAVIFFHVSEYFLAAFIHGKSSVTLKSLLISKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            LY +K+ +E  SYTACRQLSQMF+A+IFFH SEY +A  IHG+S+VTLKSLLISK YLLAM+ SLLEY  E+  FP LKE W ISN         EIIRK AI+TAG+SFTH+I+V+ +E H+LVTHGIY++IRH
Sbjct:   20 LYSSKVFSENFSYTACRQLSQMFLAIIFFHSSEYVIAVNIHGRSNVTLKSLLISKAYLLAMVCSLLEYFAEIVLFPGLKEIWWISNSGLAMVVIGEIIRKTAILTAGRSFTHLIKVYHEEDHKLVTHGIYRYIRH 154

[Close]

BLAST of GT618730 vs. TrEMBL
Match: D7TES1_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_42.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00026135001 PE=4 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 3.611e-41
Identity = 82/129 (63.57%), Postives = 102/129 (79.07%), Query Frame = 3

Query:  309 MTEILSYTACRQLSQMFIAVIFFHVSEYFLAAFIHGKSSVTLKSLLISKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            MTEI S+T+CRQLSQM + ++ FH SEY LA  IHGKS+VT   LLISKHYLLA+I SLLEYL+E+  FP LKE+W ISN         EIIRK+AI+TA Q+FTHMIR+H DE+H+LVT+G+Y+F+RH
Sbjct:    1 MTEIFSHTSCRQLSQMLMTILIFHSSEYILAVAIHGKSNVTRGDLLISKHYLLALICSLLEYLLEIFLFPGLKEFWWISNLGLVMVVIGEIIRKIAILTASQAFTHMIRIHRDEQHKLVTYGVYRFVRH 129

[Close]

BLAST of GT618730 vs. TrEMBL
Match: B9T024_RICCO (Protein-s isoprenylcysteine O-methyltransferase, putative OS=Ricinus communis GN=RCOM_0037200 PE=4 SV=1)

HSP 1 Score: 171.785 bits (434), Expect = 4.716e-41
Identity = 80/129 (62.02%), Postives = 102/129 (79.07%), Query Frame = 3

Query:  309 MTEILSYTACRQLSQMFIAVIFFHVSEYFLAAFIHGKSSVTLKSLLISKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            MTE   YTACRQLSQMF A+IFFH+SEY LA  IHGKS+V+LKSLL+SK YLLAMI SLLEY +E+  FP LKE W +SN         E+IRKMAI+TAG++FTH+I+++ +E H+L+T G+Y+F+RH
Sbjct:    1 MTESFGYTACRQLSQMFSAIIFFHISEYLLAVGIHGKSNVSLKSLLVSKKYLLAMICSLLEYFIEIYLFPGLKEDWWVSNLGLALVIIGELIRKMAIITAGRAFTHLIKIYHEEHHKLITSGVYRFVRH 129

[Close]

BLAST of GT618730 vs. TrEMBL
Match: D7M1B0_ARALY (Prenylcysteine alpha-carboxyl methyltransferase OS=Arabidopsis lyrata subsp. lyrata GN=ATSTE14B PE=4 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 7.529e-39
Identity = 78/129 (60.47%), Postives = 98/129 (75.97%), Query Frame = 3

Query:  309 MTEILSYTACRQLSQMFIAVIFFHVSEYFLAAFIHGKSSVTLKSLLISKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            MTEI S T  RQL+QMF+A+IFFH SEY LA  IHG S VTL SLLISKHY LAM+ S+LEY+ E+ FFP LK++W ISN         EIIRK+AI+TAG+SFTH+I++  +E H+LVT G+Y+ +RH
Sbjct:    1 MTEIFSDTGFRQLTQMFLAIIFFHTSEYILAIAIHGASKVTLSSLLISKHYALAMLISVLEYITEIVFFPRLKQHWWISNFGLIMIILGEIIRKIAIITAGRSFTHLIKIRREEHHRLVTEGVYQIMRH 129

[Close]

BLAST of GT618730 vs. TrEMBL
Match: A5BNM5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_038323 PE=4 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 7.529e-39
Identity = 82/129 (63.57%), Postives = 99/129 (76.74%), Query Frame = 3

Query:  309 MTEILSYTACRQLSQMFIAVIFFHVSEYFLAAFIHGKSSVTLKSLLISKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            MTE  SYTACRQLSQMF+A++FFH SEY +A  IHG+S+VT KS+LISK YLL M+ SLLEY VE+  FP LKE W ISN         EIIRK AI+TAG++FT +IRVH +E H+LVTHGIY ++RH
Sbjct:    1 MTENFSYTACRQLSQMFLAILFFHSSEYVIAVTIHGRSNVTRKSILISKVYLLEMVCSLLEYFVEIVLFPGLKEIWWISNSGLAMVVIGEIIRKTAILTAGRAFTLVIRVHYEEDHKLVTHGIYGYMRH 129

[Close]

BLAST of GT618730 vs. TrEMBL
Match: D7M1Q6_ARALY (Prenylcysteine alpha-carboxyl methyltransferase 14A OS=Arabidopsis lyrata subsp. lyrata GN=ATSTE14 PE=4 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 3.737e-38
Identity = 78/129 (60.47%), Postives = 97/129 (75.19%), Query Frame = 3

Query:  309 MTEILSYTACRQLSQMFIAVIFFHVSEYFLAAFIHGKSSVTLKSLLISKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            MTEI S T  RQLSQM +++IFFH SEY LA  IHG S+VTL SLLISKHY LAM+ SLLEYL E+  FP LK++W +SN         EIIRK AI+TAG+SFTH+I+++ +E H LVTHG+Y+ +RH
Sbjct:    1 MTEIFSDTGIRQLSQMLLSLIFFHTSEYILAIAIHGASNVTLSSLLISKHYALAMLVSLLEYLTEIILFPGLKQHWWVSNFGLIMIIVGEIIRKAAIITAGRSFTHLIKINHEEHHALVTHGVYRLMRH 129

[Close]

BLAST of GT618730 vs. SwissProt
Match: ICMTB_ARATH (Protein-S-isoprenylcysteine O-methyltransferase B OS=Arabidopsis thaliana GN=ICMTB PE=1 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 2.510e-39
Identity = 77/129 (59.69%), Postives = 97/129 (75.19%), Query Frame = 3

Query:  309 MTEILSYTACRQLSQMFIAVIFFHVSEYFLAAFIHGKSSVTLKSLLISKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            MTEI S T  RQL+QMF+A+IFFH SEY LA  IHG S VTL SLLISKHY LAM+ S+LEY+ E+ FFP LK++W ISN         EI+RK AI+TAG+SFTH+I++  +E H+LVT G+Y+ +RH
Sbjct:    1 MTEIFSDTGFRQLTQMFLAIIFFHTSEYILAIAIHGASKVTLSSLLISKHYALAMLISVLEYIAEIVFFPGLKQHWWISNFGLTMIILGEILRKTAIITAGRSFTHLIKIRREEHHKLVTEGVYQIMRH 129

[Close]

BLAST of GT618730 vs. SwissProt
Match: ICMTA_ARATH (Protein-S-isoprenylcysteine O-methyltransferase A OS=Arabidopsis thaliana GN=ICMTA PE=1 SV=1)

HSP 1 Score: 162.155 bits (409), Expect = 2.510e-39
Identity = 77/129 (59.69%), Postives = 99/129 (76.74%), Query Frame = 3

Query:  309 MTEILSYTACRQLSQMFIAVIFFHVSEYFLAAFIHGKSSVTLKSLLISKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            MTEI S T+ RQLSQM +++IFFH+SEY LA  IHG S+VTL SLLI+KHY LAM+ SLLEYL E+  FP LK++W +SN         EIIRK AI+TAG+SFTH+I+++ +E H LVTHG+Y+ +RH
Sbjct:    1 MTEIFSDTSIRQLSQMLLSLIFFHISEYILAITIHGASNVTLSSLLITKHYALAMLLSLLEYLTEIILFPGLKQHWWVSNFGLIMIIVGEIIRKAAIITAGRSFTHLIKINYEEHHGLVTHGVYRLMRH 129

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BLAST of GT618730 vs. SwissProt
Match: ICMT_ORYSJ (Probable protein-S-isoprenylcysteine O-methyltransferase OS=Oryza sativa subsp. japonica GN=ICMT PE=2 SV=2)

HSP 1 Score: 136.346 bits (342), Expect = 1.475e-31
Identity = 61/111 (54.95%), Postives = 84/111 (75.68%), Query Frame = 3

Query:  363 AVIFFHVSEYFLAAFIHGKSSVTLKSLLISKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            A++ FH SEY LAA  HG+ +VT  SLLISK Y+LAM F++LE+L E   FPELKEYW +S          E+IRK+A+VTAG+SFTH+IR+H +++H+L+THG+Y+ +RH
Sbjct:   13 ALVIFHGSEYVLAAAFHGRRNVTATSLLISKQYVLAMSFAMLEHLTEALLFPELKEYWFVSYVGLVMVIIGEVIRKLAVVTAGRSFTHVIRIHYEDQHKLITHGVYRLMRH 123

[Close]

BLAST of GT618730 vs. SwissProt
Match: ICMT_ORYSI (Probable protein-S-isoprenylcysteine O-methyltransferase OS=Oryza sativa subsp. indica GN=ICMT PE=3 SV=2)

HSP 1 Score: 136.346 bits (342), Expect = 1.475e-31
Identity = 61/111 (54.95%), Postives = 84/111 (75.68%), Query Frame = 3

Query:  363 AVIFFHVSEYFLAAFIHGKSSVTLKSLLISKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            A++ FH SEY LAA  HG+ +VT  SLLISK Y+LAM F++LE+L E   FPELKEYW +S          E+IRK+A+VTAG+SFTH+IR+H +++H+L+THG+Y+ +RH
Sbjct:   13 ALVIFHGSEYVLAAAFHGRRNVTATSLLISKQYVLAMSFAMLEHLTEALLFPELKEYWFVSYVGLVMVIIGEVIRKLAVVTAGRSFTHVIRIHYEDQHKLITHGVYRLMRH 123

[Close]

BLAST of GT618730 vs. SwissProt
Match: ICMT_XENLA (Protein-S-isoprenylcysteine O-methyltransferase OS=Xenopus laevis GN=icmt PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.387e-13
Identity = 39/110 (35.45%), Postives = 65/110 (59.09%), Query Frame = 3

Query:  372 FFHVSEYFLAAFIHGKSSVTLKSLLI--SKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            FFH SEY + A ++   S+++ S L+  S  Y LA + S +E+ +E T +P+LK+   +S          E++RK A++TAG +F H+++    + H LVT G+Y + RH
Sbjct:  106 FFHYSEYLVTA-MNNPRSLSIDSFLLNHSLEYTLAALSSWVEFTIETTIYPDLKQITWLSVIGLIMVLFGEVLRKCAMLTAGSNFNHIVQNEKSDSHTLVTSGVYSWFRH 214

[Close]

BLAST of GT618730 vs. SwissProt
Match: ICMT_HUMAN (Protein-S-isoprenylcysteine O-methyltransferase OS=Homo sapiens GN=ICMT PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 3.416e-12
Identity = 40/109 (36.70%), Postives = 62/109 (56.88%), Query Frame = 3

Query:  375 FHVSEYFLAAFIHGKSSVTLKSLLI--SKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            FH SEY + A ++   S++L S L+  S  Y +A + S LE+ +E  F+PELK+   +S          E +RK A+ TAG +F H+++    + H LVT G+Y + RH
Sbjct:  103 FHYSEYLVTA-VNNPKSLSLDSFLLNHSLEYTVAALSSWLEFTLENIFWPELKQITWLSVTGLLMVVFGECLRKAAMFTAGSNFNHVVQNEKSDTHTLVTSGVYAWFRH 210

[Close]

BLAST of GT618730 vs. SwissProt
Match: ICMT_MOUSE (Protein-S-isoprenylcysteine O-methyltransferase OS=Mus musculus GN=Icmt PE=2 SV=2)

HSP 1 Score: 71.633 bits (174), Expect = 4.461e-12
Identity = 39/109 (35.78%), Postives = 62/109 (56.88%), Query Frame = 3

Query:  375 FHVSEYFLAAFIHGKSSVTLKSLLI--SKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            FH SEY + A ++   S++L S L+  S  Y +A + S +E+ +E  F+PELK+   +S          E +RK A+ TAG +F H+++    + H LVT G+Y + RH
Sbjct:  102 FHYSEYLVTA-VNNPKSLSLDSFLLNHSLEYTVAALSSWIEFTLENIFWPELKQITWLSATGLLMVVFGECLRKAAMFTAGSNFNHVVQSEKSDTHTLVTSGVYAWCRH 209

[Close]

BLAST of GT618730 vs. SwissProt
Match: ICMT_RAT (Protein-S-isoprenylcysteine O-methyltransferase (Fragment) OS=Rattus norvegicus GN=Icmt PE=1 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 5.826e-12
Identity = 39/109 (35.78%), Postives = 62/109 (56.88%), Query Frame = 3

Query:  375 FHVSEYFLAAFIHGKSSVTLKSLLI--SKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            FH SEY L   ++   S++L S L+  S  Y +A + S +E+ +E  F+PELK+   +S          E +RK+A+ TAG +F H+++    + H LVT G+Y + RH
Sbjct:   51 FHYSEY-LVTTVNNPKSLSLDSFLLNHSLEYTVAALSSWIEFTLENIFWPELKQITWLSAAGLLMVIFGECLRKVAMFTAGSNFNHVVQSEKSDTHTLVTSGVYAWCRH 158

[Close]

BLAST of GT618730 vs. SwissProt
Match: ICMT_DICDI (Protein-S-isoprenylcysteine O-methyltransferase OS=Dictyostelium discoideum GN=icmt-1 PE=2 SV=2)

HSP 1 Score: 67.0106 bits (162), Expect = 1.099e-10
Identity = 38/112 (33.93%), Postives = 63/112 (56.25%), Query Frame = 3

Query:  375 FHVSEYFLAAFIHGKSSVTLKSLLI--SKHYLLAMIFSLLEYLVEVTFFPELKEY---WVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            FH+ EY      H    ++ KS L+  S  + +A++ S +E+ +E  FFP LK +   WV            +I+R +A+ TAG +FTH+++    + H LVT+GIY+++RH
Sbjct:   63 FHMWEYIWVTMYH-PDKLSSKSFLLNHSPQFNMALLISFIEFWIEWYFFPSLKTFSLWWV----GAICMVFGQIVRSVAMDTAGSNFTHLVQEEKRDDHVLVTNGIYQYMRH 169

[Close]

BLAST of GT618730 vs. SwissProt
Match: STE14_YEAST (Protein-S-isoprenylcysteine O-methyltransferase OS=Saccharomyces cerevisiae GN=STE14 PE=1 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 3.908e-8
Identity = 40/127 (31.50%), Postives = 65/127 (51.18%), Query Frame = 3

Query:  339 RQLSQMFIAVIFFHVSEYFLAAFIHGKSSVTLKSLLIS--KHYLLAMIFSLLEYLVEVTFFPELK--EYWVISNXXXXXXXXXEII----RKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            +  +   IA+  FH  EY++ A  +    V  +S L++  K Y+ A  F++LE LVE   FP+LK   Y + +           I+    R +A+ TAG SF+H+++   +  H LV  G+Y + RH
Sbjct:   47 KNFNLFIIALSLFHFLEYYITAK-YNPLKVHSESFLLNNGKSYMAAHSFAILECLVESFLFPDLKIFSYSLATKLCTVLGCLLVILGQYTRTIAMHTAGHSFSHIVKTKKESDHVLVKTGVYSWSRH 172

[Close]

BLAST of GT618730 vs. TAIR peptide
Match: AT5G08335.1 (| Symbols: ATSTE14B, ATICMTB, ICMTB | Isoprenylcysteine carboxyl methyltransferase (ICMT) family | chr5:2683012-2683605 FORWARD LENGTH=197)

HSP 1 Score: 162.155 bits (409), Expect = 2.528e-40
Identity = 77/129 (59.69%), Postives = 97/129 (75.19%), Query Frame = 3

Query:  309 MTEILSYTACRQLSQMFIAVIFFHVSEYFLAAFIHGKSSVTLKSLLISKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            MTEI S T  RQL+QMF+A+IFFH SEY LA  IHG S VTL SLLISKHY LAM+ S+LEY+ E+ FFP LK++W ISN         EI+RK AI+TAG+SFTH+I++  +E H+LVT G+Y+ +RH
Sbjct:    1 MTEIFSDTGFRQLTQMFLAIIFFHTSEYILAIAIHGASKVTLSSLLISKHYALAMLISVLEYIAEIVFFPGLKQHWWISNFGLTMIILGEILRKTAIITAGRSFTHLIKIRREEHHKLVTEGVYQIMRH 129

[Close]

BLAST of GT618730 vs. TAIR peptide
Match: AT5G23320.1 (| Symbols: ATSTE14, STE14, ATSTE14A, ATPCM, ATICMTA, ICMTA, STE14A | homolog of yeast STE14 A | chr5:7853368-7853961 FORWARD LENGTH=197)

HSP 1 Score: 162.155 bits (409), Expect = 2.528e-40
Identity = 77/129 (59.69%), Postives = 99/129 (76.74%), Query Frame = 3

Query:  309 MTEILSYTACRQLSQMFIAVIFFHVSEYFLAAFIHGKSSVTLKSLLISKHYLLAMIFSLLEYLVEVTFFPELKEYWVISNXXXXXXXXXEIIRKMAIVTAGQSFTHMIRVHPDERHQLVTHGIYKFIRH 695
            MTEI S T+ RQLSQM +++IFFH+SEY LA  IHG S+VTL SLLI+KHY LAM+ SLLEYL E+  FP LK++W +SN         EIIRK AI+TAG+SFTH+I+++ +E H LVTHG+Y+ +RH
Sbjct:    1 MTEIFSDTSIRQLSQMLLSLIFFHISEYILAITIHGASNVTLSSLLITKHYALAMLLSLLEYLTEIILFPGLKQHWWVSNFGLIMIIVGEIIRKAAIITAGRSFTHLIKINYEEHHGLVTHGVYRLMRH 129

The following BLAST results are available for this feature:

BLAST of GT618730 vs. TrEMBL
BLAST of GT618730 vs. SwissProt
BLAST of GT618730 vs. TAIR peptide

BLAST of GT618730 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9T7U4_RICCO	3.156e-45	65.12	Protein-s isoprenylcysteine O-methyltransferase, p...	[more]
A9PB59_POPTR	3.858e-43	65.12	Putative uncharacterized protein OS=Populus tricho...	[more]
B9HGE7_POPTR	1.915e-42	64.34	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9H5P5_POPTR	1.241e-41	64.34	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7TR53_VITVI	3.611e-41	64.44	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7TES1_VITVI	3.611e-41	63.57	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9T024_RICCO	4.716e-41	62.02	Protein-s isoprenylcysteine O-methyltransferase, p...	[more]
D7M1B0_ARALY	7.529e-39	60.47	Prenylcysteine alpha-carboxyl methyltransferase OS...	[more]
A5BNM5_VITVI	7.529e-39	63.57	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7M1Q6_ARALY	3.737e-38	60.47	Prenylcysteine alpha-carboxyl methyltransferase 14...	[more]

BLAST of GT618730 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ICMTB_ARATH	2.510e-39	59.69	Protein-S-isoprenylcysteine O-methyltransferase B ...	[more]
ICMTA_ARATH	2.510e-39	59.69	Protein-S-isoprenylcysteine O-methyltransferase A ...	[more]
ICMT_ORYSJ	1.475e-31	54.95	Probable protein-S-isoprenylcysteine O-methyltrans...	[more]
ICMT_ORYSI	1.475e-31	54.95	Probable protein-S-isoprenylcysteine O-methyltrans...	[more]
ICMT_XENLA	1.387e-13	35.45	Protein-S-isoprenylcysteine O-methyltransferase OS...	[more]
ICMT_HUMAN	3.416e-12	36.70	Protein-S-isoprenylcysteine O-methyltransferase OS...	[more]
ICMT_MOUSE	4.461e-12	35.78	Protein-S-isoprenylcysteine O-methyltransferase OS...	[more]
ICMT_RAT	5.826e-12	35.78	Protein-S-isoprenylcysteine O-methyltransferase (F...	[more]
ICMT_DICDI	1.099e-10	33.93	Protein-S-isoprenylcysteine O-methyltransferase OS...	[more]
STE14_YEAST	3.908e-8	31.50	Protein-S-isoprenylcysteine O-methyltransferase OS...	[more]

BLAST of GT618730 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G08335.1	2.528e-40	59.69	\| Symbols: ATSTE14B, ATICMTB, ICMTB \| Isoprenylcys...	[more]
AT5G23320.1	2.528e-40	59.69	\| Symbols: ATSTE14, STE14, ATSTE14A, ATPCM, ATICMT...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 27..38 score: NAcoord: 41..51 score
None	No IPR available	PANTHER	PTHR12714	PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE	coord: 92..176 score: 7.4
None	No IPR available	SEG	seg	seg	coord: 128..136 score
None	No IPR available	SEG	seg	seg	coord: 17..27 score: NAcoord: 30..40 score
IPR007269	Isoprenylcysteine carboxyl methyltransferase	PFAM	PF04140	ICMT	coord: 130..176 score: 9.3

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT618730 ID=GT618730; Name=GT618730; organism=Lens culinaris; type=EST; length=697bp
TGGAATCCTATTGAATTGCCGGAAAACGCAACCGGAGTTGATCATCACCT
GCCAGAAACAGTGAGAAATTGCGGGATCTTGCGATTGCGGTTGCTGCTAC
GCGCATTGCGGTTGTGTGGTGTTGTGATTGCGGTCATTGCAGCCATTGTG
GCGTGTGTTTTGATTGAGAAGAGTTTATAATACACGTTATTAAAAAACAC
TTAATGCTAAGATTTTTCGGAGGTCTGGCTTATATCTTAGATGATTTAAG
CAACCAAGTCTTTCTGTGGCTTAAAACAAGAGAAGCTTACTGTCTTTATT
TGGCGAAAATGACGGAAATCCTAAGCTACACTGCTTGCAGACAATTATCT
CAAATGTTCATTGCAGTTATCTTCTTTCACGTTTCAGAATATTTTCTAGC
CGCCTTCATTCATGGAAAATCAAGTGTAACTTTGAAGTCTCTTTTGATTA
GCAAACACTATCTTTTAGCAATGATATTTTCATTGCTAGAATATTTAGTT
GAGGTTACTTTCTTTCCTGAGCTAAAGGAATATTGGGTTATCAGCAATTT
AGGGTTAGTACTGGTTGTTTTAGGTGAAATTATAAGAAAAATGGCAATAG
TAACAGCAGGGCAATCGTTTACTCATATGATAAGGGTTCATCCTGATGAG
CGTCACCAATTGGTTACACATGGTATTTATAAATTTATTCGCCATCC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004671	protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006481	C-terminal protein methylation

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR007269	ICMT_MeTrfase

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