GT618925

EST Overview


Name	GT618925
Unique Name	GT618925
Type	EST
Organism	Lens culinaris (lentil)
Sequence length	689

Libraries


Library Name	Type
LIBEST_025390 LCUSE1NG	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Lens culinaris unigene v1	Lens culinaris unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Lens culinaris unigene v1	2010-12-27
Homology Analysis: Lens culinaris unigene v1 vs Trembl	2010-12-28
Homology Analysis: Lens culinaris unigene v1 vs Swissprot	2010-12-28
KEGG analysis for Lens culinaris unigene v1	2011-01-05
Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Lens culinaris unigene v1	2011-01-06

Homology

[Close]

BLAST of GT618925 vs. TrEMBL
Match: D7SNX4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_23.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00018752001 PE=4 SV=1)

HSP 1 Score: 250.366 bits (638), Expect = 1.022e-64
Identity = 124/172 (72.09%), Postives = 142/172 (82.56%), Query Frame = -1

Query:  174 LKVRGNYYISIDNVGAGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKVELKKLFATKTIKELKEVSLSANQEKSEIKKMTWKVEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFLLKF 689
            L VRGNYY+SID +G G++WRRTTGQEIYSPLLLAFTHE  E W +SHLTKGTVM+PNYSLP NVA+ITL+ELD G VLLRLAHLYE  EDA+YSTLAKVEL+K+F  K IKE++E +LS NQEKSE+K + WKVEGD   E   +RGG V  S  VVELGPMEIRTFLL+F
Sbjct:  835 LTVRGNYYMSIDLLGDGAQWRRTTGQEIYSPLLLAFTHEKLETWTASHLTKGTVMEPNYSLPLNVAVITLQELDDGSVLLRLAHLYEAGEDAKYSTLAKVELQKMFRGKKIKEIRETNLSTNQEKSEMKTLKWKVEGDNGDEIAPLRGGPVDNSTLVVELGPMEIRTFLLEF 1006

[Close]

BLAST of GT618925 vs. TrEMBL
Match: B9GYZ5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_757134 PE=4 SV=1)

HSP 1 Score: 250.366 bits (638), Expect = 1.022e-64
Identity = 125/172 (72.67%), Postives = 142/172 (82.56%), Query Frame = -1

Query:  174 LKVRGNYYISIDNVGAGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKVELKKLFATKTIKELKEVSLSANQEKSEIKKMTWKVEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFLLKF 689
            L +RGNYY+SI+ VGAG+ WRRTTGQEIY+PLL AFT E  E  K+SH+  GT MDP YSLP NVALITL+ELD G VLLRLAHLYE  EDA YSTLA VELKK+F+ K IKELKE+SLS+NQEKSE+KKMTWKVEGD  ++P  VRGG V +S  VVELGPMEIRTFLL+F
Sbjct:  840 LTIRGNYYLSINQVGAGAVWRRTTGQEIYTPLLAAFTQEKEETSKASHVATGTAMDPGYSLPLNVALITLQELDDGSVLLRLAHLYEAGEDAAYSTLATVELKKMFSGKAIKELKEMSLSSNQEKSEMKKMTWKVEGDNGEQPSPVRGGPVDSSTLVVELGPMEIRTFLLQF 1011

[Close]

BLAST of GT618925 vs. TrEMBL
Match: A5B9Y9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_040416 PE=4 SV=1)

HSP 1 Score: 250.366 bits (638), Expect = 1.022e-64
Identity = 124/172 (72.09%), Postives = 142/172 (82.56%), Query Frame = -1

Query:  174 LKVRGNYYISIDNVGAGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKVELKKLFATKTIKELKEVSLSANQEKSEIKKMTWKVEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFLLKF 689
            L VRGNYY+SID +G G++WRRTTGQEIYSPLLLAFTHE  E W +SHLTKGTVM+PNYSLP NVA+ITL+ELD G VLLRLAHLYE  EDA+YSTLAKVEL+K+F  K IKE++E +LS NQEKSE+K + WKVEGD   E   +RGG V  S  VVELGPMEIRTFLL+F
Sbjct: 2082 LTVRGNYYMSIDLLGDGAQWRRTTGQEIYSPLLLAFTHEKLETWTASHLTKGTVMEPNYSLPLNVAVITLQELDDGSVLLRLAHLYEAGEDAKYSTLAKVELQKMFRGKKIKEIRETNLSTNQEKSEMKTLKWKVEGDNGDEIAPLRGGPVDNSTLVVELGPMEIRTFLLEF 2253

[Close]

BLAST of GT618925 vs. TrEMBL
Match: Q9FKW9_ARATH (Alpha-mannosidase OS=Arabidopsis thaliana GN=At5g66150 PE=4 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 5.433e-58
Identity = 115/173 (66.47%), Postives = 135/173 (78.03%), Query Frame = -1

Query:  174 LKVRGNYYISIDNVGAGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKVELKKLFATKTIKELKEVSLSANQEKSEIK-KMTWKVEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFLLKF 689
            L +RGNYY+SI+ VG G RWRR TGQEIYSPLL+AF HEN E WK+S+  KG  MD  Y+LP N+ALITLEELD G VLLRLAHLYE  ED+ YS +AKVELKKLF+ K IKE+ E+SLSANQEK ++K KM WKVEG+  Q    +RGG V  S  VVELGPMEIRTF+++F
Sbjct:  863 LTIRGNYYVSINKVGEGGRWRRETGQEIYSPLLMAFAHENKEKWKASNTVKGYAMDHLYTLPQNIALITLEELDLGNVLLRLAHLYEAGEDSDYSKIAKVELKKLFSGKMIKEVTEMSLSANQEKVKMKEKMKWKVEGEAEQPSSPLRGGPVDKSTLVVELGPMEIRTFVVQF 1035

[Close]

BLAST of GT618925 vs. TrEMBL
Match: D7MN08_ARALY (Glycosyl hydrolase family 38 protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_497001 PE=4 SV=1)

HSP 1 Score: 222.246 bits (565), Expect = 2.981e-56
Identity = 110/173 (63.58%), Postives = 133/173 (76.88%), Query Frame = -1

Query:  174 LKVRGNYYISIDNVGAGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKVELKKLFATKTIKELKEVSLSANQEKSEIK-KMTWKVEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFLLKF 689
            L VRGNYY+SI+ +G G RWRR TGQE+YSPLL+AF HEN E WK+S+  KG  MDP Y+LP N ALITLE +D G VL+RLAHLYE  ED+ YS  AKVELKKLF+ K IKE+ E+SLS NQEK+++K KM WKVEG+  +     RGG+V  S  VVELGPMEIRTF+++F
Sbjct:  862 LTVRGNYYVSINRIGEGGRWRRETGQELYSPLLMAFAHENKEKWKASNTVKGYAMDPLYTLPQNTALITLEGMDFGNVLVRLAHLYEAGEDSDYSKTAKVELKKLFSGKMIKEVTEMSLSGNQEKAKMKEKMKWKVEGEAEEASSPQRGGAVDKSTLVVELGPMEIRTFVVEF 1034

[Close]

BLAST of GT618925 vs. TrEMBL
Match: B4FGT7_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 218.009 bits (554), Expect = 5.622e-55
Identity = 102/172 (59.30%), Postives = 133/172 (77.33%), Query Frame = -1

Query:  174 LKVRGNYYISIDNVGAGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKVELKKLFATKTIKELKEVSLSANQEKSEIKKMTWKVEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFLLKF 689
            L VRG YY+ +D +G G+ WRRT GQ++YSP LLAFTHE+  +WKS ++ K + MD NYSLP NVA+ITL+ LD    LLRLAHL++  EDAQYS +AKVEL+++F  +TIKEL E +LSANQ+KSE+KK+ W+V G+ + +P  ++GG V +   VVELGPMEIRTFLLKF
Sbjct:  240 LTVRGTYYVKVDKIGHGAHWRRTYGQQVYSPYLLAFTHEDETSWKSYNVAKQSTMDENYSLPDNVAIITLQNLDDDTTLLRLAHLFQDLEDAQYSVIAKVELRRVFGKRTIKELAETNLSANQKKSEMKKLNWRVLGNDKSDPIPLKGGPVDSQALVVELGPMEIRTFLLKF 411

[Close]

BLAST of GT618925 vs. TrEMBL
Match: C5WP50_SORBI (Putative uncharacterized protein Sb01g026405 OS=Sorghum bicolor GN=Sb01g026405 PE=4 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 2.612e-52
Identity = 101/172 (58.72%), Postives = 130/172 (75.58%), Query Frame = -1

Query:  174 LKVRGNYYISIDNVGAGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKVELKKLFATKTIKELKEVSLSANQEKSEIKKMTWKVEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFLLKF 689
            L  RG YYI+++ +G G+ WRRT GQ++YSP LLAFTHE+A + KS ++ K T+MD NYSLP NVA+ITL+ LD G  LLRLAHL++  ED +YS +AKV+LKK+F  +TIKEL E +LSANQ+KSE+KK+ W+V GD      +V+GG V     VV LGPMEIRTFLL+F
Sbjct:  703 LTARGTYYINVEKLGHGAHWRRTYGQQVYSPFLLAFTHEDATSAKSYNVAKATMMDVNYSLPDNVAIITLQNLDDGTTLLRLAHLFQAGEDPKYSVVAKVDLKKVFGRRTIKELTETNLSANQKKSEMKKLKWRVIGDMENGTTSVKGGPVDCRALVVALGPMEIRTFLLRF 874

[Close]

BLAST of GT618925 vs. TrEMBL
Match: Q10A56_ORYSJ (Glycosyl hydrolase family 38 protein, putative, expressed OS=Oryza sativa subsp. japonica GN=Os10g0140200 PE=4 SV=1)

HSP 1 Score: 208.379 bits (529), Expect = 4.455e-52
Identity = 96/172 (55.81%), Postives = 129/172 (75.00%), Query Frame = -1

Query:  174 LKVRGNYYISIDNVGAGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKVELKKLFATKTIKELKEVSLSANQEKSEIKKMTWKVEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFLLKF 689
            L  R  YYI+++  G G+ WRRT  Q++YSP L+AF HE+  +WKS+++ K + ++ NYSLP NVA+ITL+ LD G  LLRLAHL++  ED QYS +AKVEL+KLF  + IK+L E SLSANQ+KSE+KK+ W+V G+ + +P  ++GG V +   VVELGPMEIRTFLLKF
Sbjct:  833 LVARATYYINVNKKGHGAHWRRTYSQQVYSPFLVAFAHEDERSWKSNNIAKASTVEGNYSLPDNVAIITLQSLDDGTTLLRLAHLFQAQEDTQYSVMAKVELRKLFGKRIIKDLTETSLSANQKKSEMKKLNWRVTGESKTDPAPLKGGPVDSHALVVELGPMEIRTFLLKF 1004

[Close]

BLAST of GT618925 vs. TrEMBL
Match: Q10A55_ORYSJ (Glycosyl hydrolase family 38 protein, putative, expressed OS=Oryza sativa subsp. japonica GN=LOC_Os10g05069 PE=4 SV=1)

HSP 1 Score: 208.379 bits (529), Expect = 4.455e-52
Identity = 96/172 (55.81%), Postives = 129/172 (75.00%), Query Frame = -1

Query:  174 LKVRGNYYISIDNVGAGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKVELKKLFATKTIKELKEVSLSANQEKSEIKKMTWKVEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFLLKF 689
            L  R  YYI+++  G G+ WRRT  Q++YSP L+AF HE+  +WKS+++ K + ++ NYSLP NVA+ITL+ LD G  LLRLAHL++  ED QYS +AKVEL+KLF  + IK+L E SLSANQ+KSE+KK+ W+V G+ + +P  ++GG V +   VVELGPMEIRTFLLKF
Sbjct:  702 LVARATYYINVNKKGHGAHWRRTYSQQVYSPFLVAFAHEDERSWKSNNIAKASTVEGNYSLPDNVAIITLQSLDDGTTLLRLAHLFQAQEDTQYSVMAKVELRKLFGKRIIKDLTETSLSANQKKSEMKKLNWRVTGESKTDPAPLKGGPVDSHALVVELGPMEIRTFLLKF 873

[Close]

BLAST of GT618925 vs. TrEMBL
Match: Q10A54_ORYSJ (Glycosyl hydrolase family 38 protein, putative, expressed OS=Oryza sativa subsp. japonica GN=LOC_Os10g05069 PE=4 SV=1)

HSP 1 Score: 208.379 bits (529), Expect = 4.455e-52
Identity = 96/172 (55.81%), Postives = 129/172 (75.00%), Query Frame = -1

Query:  174 LKVRGNYYISIDNVGAGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKVELKKLFATKTIKELKEVSLSANQEKSEIKKMTWKVEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFLLKF 689
            L  R  YYI+++  G G+ WRRT  Q++YSP L+AF HE+  +WKS+++ K + ++ NYSLP NVA+ITL+ LD G  LLRLAHL++  ED QYS +AKVEL+KLF  + IK+L E SLSANQ+KSE+KK+ W+V G+ + +P  ++GG V +   VVELGPMEIRTFLLKF
Sbjct:  644 LVARATYYINVNKKGHGAHWRRTYSQQVYSPFLVAFAHEDERSWKSNNIAKASTVEGNYSLPDNVAIITLQSLDDGTTLLRLAHLFQAQEDTQYSVMAKVELRKLFGKRIIKDLTETSLSANQKKSEMKKLNWRVTGESKTDPAPLKGGPVDSHALVVELGPMEIRTFLLKF 815

[Close]

BLAST of GT618925 vs. SwissProt
Match: MA2B1_MOUSE (Lysosomal alpha-mannosidase OS=Mus musculus GN=Man2b1 PE=2 SV=3)

HSP 1 Score: 68.5514 bits (166), Expect = 3.713e-11
Identity = 54/171 (31.58%), Postives = 81/171 (47.37%), Query Frame = -1

Query:  183 KVRGNYYISIDNVG-AGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKVEL--KKLFATKTIKELKEVSLSANQEKSEIKKMTWKVEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFL 686
            KVRG + + + +V  A +R R    QE+ +P ++  +   +  + S    K         LPP V L+TL      ++LLRL H +   ED+  +  + V L  + LF T TI  L+E +L+ANQ  S   ++ W         P+  +    S     V L PMEIRTFL
Sbjct:  838 KVRGRHLVLLSSVSDAAARHRLLAEQEVLAPQVV-LSLGGSSPYHSRATPKTQFSGLRQELPPQVHLLTLARWGPKMLLLRLEHQFALKEDSDRNLSSPVTLNVQNLFQTFTINYLQETTLAANQPLSRASRLKWMTNTGPTSFPEPSKLDPTS-----VTLKPMEIRTFL 1002

[Close]

BLAST of GT618925 vs. SwissProt
Match: MA2B1_CAVPO (Lysosomal alpha-mannosidase OS=Cavia porcellus GN=MAN2B1 PE=1 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 2.406e-10
Identity = 51/172 (29.65%), Postives = 80/172 (46.51%), Query Frame = -1

Query:  183 VRGNYYISIDNVG-AGSRWRRTTGQEIYSPLLLAFTHENAENWKSSH--LTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQ--YSTLAKVELKKLFATKTIKELKEVSLSANQEKSEIKKMTWKVEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFL 683
            VRG + + +D    A +  R    +E+ +P L+    +        H  + +         LPP+V L+TL       +LLRL H +   ED+    S    ++L+ LF+T TI  L+E +L+ANQ ++   ++ W  E D    P   R    S     + L PMEIRTF+
Sbjct:  832 VRGRHLLLLDTAREAAAEHRLLAEKELLAPQLVLAPGQGPSYHHDHHEAVPRKQFSGLRRQLPPSVRLLTLARWGPDTLLLRLEHQFALGEDSSRNLSLPVTLDLQDLFSTFTITRLQETTLAANQLRASASRLKWTTEIDPISRPAVPRLDPSS-----ITLQPMEIRTFV 998

[Close]

BLAST of GT618925 vs. SwissProt
Match: MA2B1_BOVIN (Lysosomal alpha-mannosidase OS=Bos taurus GN=MAN2B1 PE=1 SV=3)

HSP 1 Score: 63.1586 bits (152), Expect = 1.560e-9
Identity = 49/172 (28.49%), Postives = 78/172 (45.35%), Query Frame = -1

Query:  183 LKVRGNYYISIDNVG-AGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDA--QYSTLAKVELKKLFATKTIKELKEVSLSANQEKSEIKKMTWKVEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFL 689
            L VRG + + +D    A +R R     E+ +P ++         ++     +         LPP+V L+TL       +LLRL H +   ED+    S+   ++L  LF+  TI  L+E +L+ANQ  +   ++ W  +      P   R  S +     + L PMEIRTFL
Sbjct:  825 LWVRGRHLVLLDKKETAAARHRLQAEMEVLAPQVV-LAQGGGARYRLEKAPRTQFSGLRRELPPSVRLLTLARWGPETLLLRLEHQFAVGEDSGRNLSSPVTLDLTNLFSAFTITNLRETTLAANQLLAYASRLQWTTDTGPTPHPSPSRPVSAT-----ITLQPMEIRTFL 990

[Close]

BLAST of GT618925 vs. SwissProt
Match: MANA_DICDI (Lysosomal alpha-mannosidase OS=Dictyostelium discoideum GN=manA PE=1 SV=2)

HSP 1 Score: 62.3882 bits (150), Expect = 2.661e-9
Identity = 42/155 (27.10%), Postives = 78/155 (50.32%), Query Frame = -1

Query:  180 RTTGQEIYSPLLLAFT--HENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDG--GIVLLRLAHLYE-PSEDAQYSTLAKVELKKLFATKTIKELKEVSLSANQEKSEIKKMTWK-VEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFLL 626
            R     +  PLL  FT   +++ +W S +    + +     LP  + + TL+ LD     +LLR+ ++Y+   +D+Q      ++L  +F+T TI    E++L+  Q+ S + ++ WK V+G       +      S++ FV    PM+IRTF++
Sbjct:  853 RPAALSLSHPLLPMFTTTQQSSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLSTIFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSNGFVFTFSPMQIRTFII 1007

[Close]

BLAST of GT618925 vs. SwissProt
Match: MA2B1_HUMAN (Lysosomal alpha-mannosidase OS=Homo sapiens GN=MAN2B1 PE=1 SV=3)

HSP 1 Score: 61.6178 bits (148), Expect = 4.538e-9
Identity = 55/173 (31.79%), Postives = 84/173 (48.55%), Query Frame = -1

Query:  183 VRGNYYISIDNVGAGSRWRRTTG-QEIYSP-LLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKV--ELKKLFATKTIKELKEVSLSANQEKSEIKKMTWKVE--GDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFL 683
            VRG + + +D   A +   R    QE+ +P ++LA     A N  +   T+ + +     LPP+V L+TL      +VLLRL H +   ED+  +  A V   L+ LF+T TI  L+E +L ANQ +    ++ W         Q P  +   +++       L PMEIRTFL
Sbjct:  838 VRGRHLVLLDTAQAAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGL--RRDLPPSVHLLTLASWGPEMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQLREAASRLKWTTNTGPTPHQTPYQLDPANIT-------LEPMEIRTFL 1001

[Close]

BLAST of GT618925 vs. SwissProt
Match: MA2B1_MACFA (Lysosomal alpha-mannosidase OS=Macaca fascicularis GN=MAN2B1 PE=2 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.011e-8
Identity = 54/173 (31.21%), Postives = 84/173 (48.55%), Query Frame = -1

Query:  183 VRGNYYISIDNVGAGSRWRRTTG-QEIYSP-LLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKV--ELKKLFATKTIKELKEVSLSANQEKSEIKKMTWKVE--GDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFL 683
            VRG + + +D   A +   R    QE+ +P ++LA     A N  +   T+ + +     LPP+V L+TL      ++LLRL H +   ED+  +  A V   L+ LF+T TI  L+E +L ANQ +    ++ W         Q P  +   +++       L PMEIRTFL
Sbjct:  839 VRGRHLVLLDTAQAAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGL--RRELPPSVHLLTLASWGPEMLLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQLREAASRLKWTTNTGPTPHQTPYQLDPANIT-------LEPMEIRTFL 1002

[Close]

BLAST of GT618925 vs. SwissProt
Match: MA2B1_FELCA (Lysosomal alpha-mannosidase OS=Felis catus GN=MAN2B1 PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 3.842e-8
Identity = 48/169 (28.40%), Postives = 75/169 (44.38%), Query Frame = -1

Query:  183 VRGNYYISIDNVGAGSRWRRTTGQ-EIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDA-QYSTLAKVELKKLFATKTIKELKEVSLSANQEKSEIKKMTWKVEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFL 683
            VRG + + +D V   +   R   + E+ +P ++      A  +      +         LPP+V L+TL   D   +LLRL H +   ED+   S+   ++L  LF+  TI  L+E +L ANQ ++   ++ W         P   R          + L PMEIRTFL
Sbjct:  836 VRGRHLVLLDKVRTAATGHRLQAEKEVLTPQVVLAPGGGAP-YHLKVAPRKQFSGLRRELPPSVHLLTLARWDQKTLLLRLEHQFAVGEDSGNLSSPVTLDLTDLFSAFTITYLQETTLVANQLRASASRLKWTPNTGPTPLPSPSR-----LDPATITLQPMEIRTFL 998

[Close]

BLAST of GT618925 vs. TAIR peptide
Match: AT5G66150.1 (| Symbols: | Glycosyl hydrolase family 38 protein | chr5:26439013-26444434 REVERSE LENGTH=1047)

HSP 1 Score: 228.024 bits (580), Expect = 3.695e-60
Identity = 115/173 (66.47%), Postives = 135/173 (78.03%), Query Frame = -1

Query:  174 LKVRGNYYISIDNVGAGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKVELKKLFATKTIKELKEVSLSANQEKSEIK-KMTWKVEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFLLKF 689
            L +RGNYY+SI+ VG G RWRR TGQEIYSPLL+AF HEN E WK+S+  KG  MD  Y+LP N+ALITLEELD G VLLRLAHLYE  ED+ YS +AKVELKKLF+ K IKE+ E+SLSANQEK ++K KM WKVEG+  Q    +RGG V  S  VVELGPMEIRTF+++F
Sbjct:  863 LTIRGNYYVSINKVGEGGRWRRETGQEIYSPLLMAFAHENKEKWKASNTVKGYAMDHLYTLPQNIALITLEELDLGNVLLRLAHLYEAGEDSDYSKIAKVELKKLFSGKMIKEVTEMSLSANQEKVKMKEKMKWKVEGEAEQPSSPLRGGPVDKSTLVVELGPMEIRTFVVQF 1035

[Close]

BLAST of GT618925 vs. TAIR peptide
Match: AT3G26720.1 (| Symbols: | Glycosyl hydrolase family 38 protein | chr3:9816707-9823056 FORWARD LENGTH=1019)

HSP 1 Score: 197.978 bits (502), Expect = 4.095e-51
Identity = 99/174 (56.90%), Postives = 130/174 (74.71%), Query Frame = -1

Query:  174 LKVRGNYYISIDNVGAGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKVELKKLFATKTIKELKEVSLSANQEKSEIKK--MTWKVEGDKRQEPQAVRGGSVSTSDFVVELGPMEIRTFLLKF 689
            L ++G +Y+ ID  G G++WRRT GQEIYSPLL+AFT +  ++W +SH T  +  +P+YSLP NVAL+TL+EL+ G VLLRLAHL+E  ED++YS +AKVELKKLF  K I+E+KE SLS NQEK+E++K  + WKVEG   +E +  RG +V     VVEL PMEIRT L+KF
Sbjct:  832 LTIQGKFYVQIDKPGDGAKWRRTFGQEIYSPLLIAFTEQEGDSWINSHKTTFSAFEPSYSLPKNVALLTLQELENGEVLLRLAHLFEVGEDSEYSVMAKVELKKLFHNKKIREVKETSLSGNQEKAEMEKRRLIWKVEGSAGEEVK--RGEAVDAEKLVVELVPMEIRTLLIKF 1003

[Close]

BLAST of GT618925 vs. TAIR peptide
Match: AT5G13980.2 (| Symbols: | Glycosyl hydrolase family 38 protein | chr5:4508626-4514334 FORWARD LENGTH=1024)

HSP 1 Score: 171.4 bits (433), Expect = 4.105e-43
Identity = 98/189 (51.85%), Postives = 123/189 (65.08%), Query Frame = -1

Query:  135 LKVRGNYYISIDNVGAGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKVELKKLFATKTIKELKEVSLSANQEKS--EIKKMTWKVEGDKR--QEPQAVRGGSVSTSDFVVELGPMEIRTFLLKF*EVESQILVNVFD 689
            L ++G YY  ID  G G++WRRT GQEIYSPLLLAF  ++     S      + +DP+YSLP NVAL+TL+ELD G VLLRLAHLYE  ED + S +A VELKKLF  K I +L E+SLSANQE+S  E K++ WKVEG+    +E +A RG  +      +EL PMEIRT L+      S   +N FD
Sbjct:  835 LTIQGKYYYRIDPYGEGAKWRRTFGQEIYSPLLLAFAQQDDGKPMSFGAASFSGIDPSYSLPDNVALLTLQELDDGNVLLRLAHLYEVEEDKELSGVASVELKKLFPGKKIGKLTEMSLSANQERSTMEKKRLVWKVEGEGSYGEEKKAKRGREIDPRKLEMELYPMEIRTVLIHLELPSSHSRINRFD 1023

[Close]

BLAST of GT618925 vs. TAIR peptide
Match: AT5G13980.1 (| Symbols: | Glycosyl hydrolase family 38 protein | chr5:4508626-4514334 FORWARD LENGTH=1024)

HSP 1 Score: 171.4 bits (433), Expect = 4.105e-43
Identity = 98/189 (51.85%), Postives = 123/189 (65.08%), Query Frame = -1

Query:  135 LKVRGNYYISIDNVGAGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKVELKKLFATKTIKELKEVSLSANQEKS--EIKKMTWKVEGDKR--QEPQAVRGGSVSTSDFVVELGPMEIRTFLLKF*EVESQILVNVFD 689
            L ++G YY  ID  G G++WRRT GQEIYSPLLLAF  ++     S      + +DP+YSLP NVAL+TL+ELD G VLLRLAHLYE  ED + S +A VELKKLF  K I +L E+SLSANQE+S  E K++ WKVEG+    +E +A RG  +      +EL PMEIRT L+      S   +N FD
Sbjct:  835 LTIQGKYYYRIDPYGEGAKWRRTFGQEIYSPLLLAFAQQDDGKPMSFGAASFSGIDPSYSLPDNVALLTLQELDDGNVLLRLAHLYEVEEDKELSGVASVELKKLFPGKKIGKLTEMSLSANQERSTMEKKRLVWKVEGEGSYGEEKKAKRGREIDPRKLEMELYPMEIRTVLIHLELPSSHSRINRFD 1023

[Close]

BLAST of GT618925 vs. TAIR peptide
Match: AT3G26720.2 (| Symbols: | Glycosyl hydrolase family 38 protein | chr3:9816707-9822958 FORWARD LENGTH=943)

HSP 1 Score: 139.813 bits (351), Expect = 1.323e-33
Identity = 64/109 (58.72%), Postives = 85/109 (77.98%), Query Frame = -1

Query:  363 LKVRGNYYISIDNVGAGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYEPSEDAQYSTLAKVELKKLFATK 689
            L ++G +Y+ ID  G G++WRRT GQEIYSPLL+AFT +  ++W +SH T  +  +P+YSLP NVAL+TL+EL+ G VLLRLAHL+E  ED++YS +AKVELKKLF  K
Sbjct:  832 LTIQGKFYVQIDKPGDGAKWRRTFGQEIYSPLLIAFTEQEGDSWINSHKTTFSAFEPSYSLPKNVALLTLQELENGEVLLRLAHLFEVGEDSEYSVMAKVELKKLFHNK 940

[Close]

BLAST of GT618925 vs. TAIR peptide
Match: AT5G13980.3 (| Symbols: | Glycosyl hydrolase family 38 protein | chr5:4508626-4513928 FORWARD LENGTH=921)

HSP 1 Score: 101.293 bits (251), Expect = 5.217e-22
Identity = 51/87 (58.62%), Postives = 62/87 (71.26%), Query Frame = -1

Query:  429 LKVRGNYYISIDNVGAGSRWRRTTGQEIYSPLLLAFTHENAENWKSSHLTKGTVMDPNYSLPPNVALITLEELDGGIVLLRLAHLYE 689
            L ++G YY  ID  G G++WRRT GQEIYSPLLLAF  ++     S      + +DP+YSLP NVAL+TL+ELD G VLLRLAHLYE
Sbjct:  835 LTIQGKYYYRIDPYGEGAKWRRTFGQEIYSPLLLAFAQQDDGKPMSFGAASFSGIDPSYSLPDNVALLTLQELDDGNVLLRLAHLYE 921

The following BLAST results are available for this feature:

BLAST of GT618925 vs. TrEMBL
BLAST of GT618925 vs. SwissProt
BLAST of GT618925 vs. TAIR peptide

BLAST of GT618925 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7SNX4_VITVI	1.022e-64	72.09	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9GYZ5_POPTR	1.022e-64	72.67	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A5B9Y9_VITVI	1.022e-64	72.09	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q9FKW9_ARATH	5.433e-58	66.47	Alpha-mannosidase OS=Arabidopsis thaliana GN=At5g6...	[more]
D7MN08_ARALY	2.981e-56	63.58	Glycosyl hydrolase family 38 protein OS=Arabidopsi...	[more]
B4FGT7_MAIZE	5.622e-55	59.30	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
C5WP50_SORBI	2.612e-52	58.72	Putative uncharacterized protein Sb01g026405 OS=So...	[more]
Q10A56_ORYSJ	4.455e-52	55.81	Glycosyl hydrolase family 38 protein, putative, ex...	[more]
Q10A55_ORYSJ	4.455e-52	55.81	Glycosyl hydrolase family 38 protein, putative, ex...	[more]
Q10A54_ORYSJ	4.455e-52	55.81	Glycosyl hydrolase family 38 protein, putative, ex...	[more]

BLAST of GT618925 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Lens culinaris unigene v1 vs Swissprot)
Total hits: 7


Match Name	E-value	Identity	Description
MA2B1_MOUSE	3.713e-11	31.58	Lysosomal alpha-mannosidase OS=Mus musculus GN=Man...	[more]
MA2B1_CAVPO	2.406e-10	29.65	Lysosomal alpha-mannosidase OS=Cavia porcellus GN=...	[more]
MA2B1_BOVIN	1.560e-9	28.49	Lysosomal alpha-mannosidase OS=Bos taurus GN=MAN2B...	[more]
MANA_DICDI	2.661e-9	27.10	Lysosomal alpha-mannosidase OS=Dictyostelium disco...	[more]
MA2B1_HUMAN	4.538e-9	31.79	Lysosomal alpha-mannosidase OS=Homo sapiens GN=MAN...	[more]
MA2B1_MACFA	1.011e-8	31.21	Lysosomal alpha-mannosidase OS=Macaca fascicularis...	[more]
MA2B1_FELCA	3.842e-8	28.40	Lysosomal alpha-mannosidase OS=Felis catus GN=MAN2...	[more]

BLAST of GT618925 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Lens culinaris unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT5G66150.1	3.695e-60	66.47	\| Symbols: \| Glycosyl hydrolase family 38 protein...	[more]
AT3G26720.1	4.095e-51	56.90	\| Symbols: \| Glycosyl hydrolase family 38 protein...	[more]
AT5G13980.2	4.105e-43	51.85	\| Symbols: \| Glycosyl hydrolase family 38 protein...	[more]
AT5G13980.1	4.105e-43	51.85	\| Symbols: \| Glycosyl hydrolase family 38 protein...	[more]
AT3G26720.2	1.323e-33	58.72	\| Symbols: \| Glycosyl hydrolase family 38 protein...	[more]
AT5G13980.3	5.217e-22	58.62	\| Symbols: \| Glycosyl hydrolase family 38 protein...	[more]

InterPro

Analysis Name: InterProScan analysis for Lens culinaris unigene v1
Date Performed: 2011-01-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011013	Glycoside hydrolase-type carbohydrate-binding	SUPERFAMILY	74650	Galactose mutarotase-like	coord: 5..172 score: 3.2
IPR011682	Glycosyl hydrolases 38, C-terminal	PFAM	PF07748	Glyco_hydro_38C	coord: 3..169 score: 3.8
None	No IPR available	GENE3D	2.60.40.1360	no description	coord: 54..172 score: 8.7
None	No IPR available	PANTHER	PTHR11607	ALPHA-MANNOSIDASE	coord: 1..172 score: 1.2
None	No IPR available	PANTHER	PTHR11607:SF3	LYSOSOMAL ALPHA-MANNOSIDASE (MANNOSIDASE ALPHA CLASS 2B MEMBER 1)	coord: 1..172 score: 1.2

Properties


Property Name	Value
Singlet in analysis	Lens culinaris unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GT618925 ID=GT618925; Name=GT618925; organism=Lens culinaris; type=EST; length=689bp
GAATACAAAATCAAATAGTCTGTTTATTGATATTATGGGACACTTTATTT
GTAGAAGTCACATATTATTGTGCTCTTTGCAATCAGATGTAGAACACACC
AATTTAATAGAGAGACACATGTTTATTTCTTAAAATCAAAAACATTAACG
AGGATCTGAGACTCTACTTCTTAAAACTTCAACAGGAAAGTACGAATTTC
CATAGGACCAAGTTCGACAACAAAATCTGAAGTGCTGACAGAACCTCCCC
TAACTGCTTGAGGTTCTTGTCTTTTGTCCCCTTCAACCTTCCATGTCATC
TTCTTTATTTCTGACTTCTCTTGGTTAGCTGATAAACTTACCTCTTTCAA
CTCCTTTATCGTTTTTGTGGCAAACAACTTTTTCAGCTCAACTTTGGCTA
GAGTTGAATATTGAGCATCTTCACTTGGCTCATATAGATGTGCCAAACGG
AGAAGAACAATTCCACCATCCAACTCCTCGAGAGTTATCAATGCAACATT
AGGAGGCAAGCTGTAATTTGGATCCATGACAGTTCCTTTGGTCAAATGAG
ACGACTTCCAATTTTCTGCATTCTCATGCGTGAAAGCCAACAAAAGCGGT
GAGTAAATTTCTTGACCAGTTGTACGACGCCAGCGTGAACCAGCTCCGAC
ATTGTCGATGCTGATATAGTAATTTCCTCTTACTTTTAG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0030246	carbohydrate binding
GO:0015923	mannosidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process
GO:0006013	mannose metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR011013	Gal_mutarotase_sf_dom
IPR011682	Glyco_hydro_38_C

Search form