FG529292

EST Overview


Name	FG529292
Unique Name	FG529292
Type	EST
Organism	Pisum sativum (pea)
Sequence length	323

Libraries


Library Name	Type
LIBEST_023217 MeriP Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of FG529292 vs. TrEMBL
Match: B0BLA8_LOTJA (CM0545.360.nc protein OS=Lotus japonicus GN=CM0545.360.nc PE=4 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 1.991e-25
Identity = 55/82 (67.07%), Postives = 65/82 (79.27%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFKXXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSGSL 246
            PAF  HPLQEAM+KDTLER +EP  N KEFLQ+AYIHPVFK         MSQE++++P++VQTKRQSR+NTP PSKHS SL
Sbjct:  674 PAFVQHPLQEAMMKDTLERAREPQLNYKEFLQNAYIHPVFKSDEDSDSDVMSQEFEDEPMLVQTKRQSRKNTPLPSKHSSSL 755

[Close]

BLAST of FG529292 vs. TrEMBL
Match: B9IAI0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_807630 PE=4 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 1.427e-23
Identity = 54/91 (59.34%), Postives = 66/91 (72.53%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFKXXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSGSLLSSVHDTAD 273
            PAF  +PLQEAM+KDTLER +EPN NLK FLQ+AY HPVFK           +E++++P +V TKRQSRRNTP PSKHSGS+ SS  +  D
Sbjct:  674 PAFVRYPLQEAMMKDTLERAREPNLNLKSFLQNAYSHPVFKGEDDSDSDEAPEEFEKEPDLVPTKRQSRRNTPLPSKHSGSVPSSQREAQD 764

[Close]

BLAST of FG529292 vs. TrEMBL
Match: B9GTV5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_755876 PE=4 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 1.577e-22
Identity = 55/91 (60.44%), Postives = 65/91 (71.43%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFKXXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSGSLLSSVHDTAD 273
            PAF  +PLQEAM+KDTLER KEPN NLK FLQ+AYIHPVFK           +E++++P +V TKRQSRRNTP PSKH GS  SS  +  D
Sbjct:  670 PAFVRYPLQEAMMKDTLERAKEPNLNLKSFLQNAYIHPVFKGEDDSDSDEAPEEFEKEPDLVPTKRQSRRNTPLPSKH-GSAASSQPEAQD 759

[Close]

BLAST of FG529292 vs. TrEMBL
Match: B9HZA2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_833957 PE=4 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 4.296e-20
Identity = 49/87 (56.32%), Postives = 64/87 (73.56%), Query Frame = 1

Query:    4 AFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFKXXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSGSLLSSVHD 264
            AF  +PLQEAM+KDTLER ++PNFNLK +LQ AYIHPVFK         +S++ + + V+V TKRQSR+NTPAPSK SG+   S+ +
Sbjct:  679 AFVKYPLQEAMMKDTLERARDPNFNLKAYLQDAYIHPVFKGGDDDEDDDLSKKLETESVLVPTKRQSRKNTPAPSKISGASSPSLFE 765

[Close]

BLAST of FG529292 vs. TrEMBL
Match: A9PJ83_9ROSI (Putative uncharacterized protein OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 4.296e-20
Identity = 49/87 (56.32%), Postives = 64/87 (73.56%), Query Frame = 1

Query:    4 AFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFKXXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSGSLLSSVHD 264
            AF  +PLQEAM+KDTLER ++PNFNLK +LQ AYIHPVFK         +S++ + + V+V TKRQSR+NTPAPSK SG+   S+ +
Sbjct:  159 AFVKYPLQEAMMKDTLERARDPNFNLKAYLQDAYIHPVFKGGDGDEDDDLSKKLETESVLVPTKRQSRKNTPAPSKISGASSPSLFE 245

[Close]

BLAST of FG529292 vs. TrEMBL
Match: Q9XEA1_ARATH (Putative uncharacterized protein AT4g04340 OS=Arabidopsis thaliana GN=AT4g04340 PE=1 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.250e-19
Identity = 48/80 (60.00%), Postives = 58/80 (72.50%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFKXXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSG 240
            PAF  +PLQEAM+KDTLER +EPN NLK +LQ AYIHPVFK         M  + + + ++V TKRQSRRNTPAPS+ SG
Sbjct:  679 PAFVRYPLQEAMMKDTLERAREPNLNLKGYLQDAYIHPVFKGGDNDDDGDMIGKLENEVIIVPTKRQSRRNTPAPSRISG 758

[Close]

BLAST of FG529292 vs. TrEMBL
Match: Q93ZR7_ARATH (Putative uncharacterized protein At4g04340 OS=Arabidopsis thaliana GN=At4g04340 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 1.250e-19
Identity = 48/80 (60.00%), Postives = 58/80 (72.50%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFKXXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSG 240
            PAF  +PLQEAM+KDTLER +EPN NLK +LQ AYIHPVFK         M  + + + ++V TKRQSRRNTPAPS+ SG
Sbjct:  679 PAFVRYPLQEAMMKDTLERAREPNLNLKGYLQDAYIHPVFKGGDNDDDGDMIGKLENEVIIVPTKRQSRRNTPAPSRISG 758

[Close]

BLAST of FG529292 vs. TrEMBL
Match: B9N8E7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_811713 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.132e-19
Identity = 51/90 (56.67%), Postives = 64/90 (71.11%), Query Frame = 1

Query:    4 AFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFK--XXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSGSLLSSVHDT 267
            AF  +PLQEAM+KDTLER ++PNFNLK +LQSAY+HPVFK           +S + + + V+V TKRQSRRNTPAPSK SG    S+ +T
Sbjct:  710 AFVKYPLQEAMMKDTLERARDPNFNLKAYLQSAYVHPVFKGGDDDIDEDDLLSGKMETESVLVPTKRQSRRNTPAPSKISGGSSPSLPET 799

[Close]

BLAST of FG529292 vs. TrEMBL
Match: Q5JLH8_ORYSJ (Os01g0534900 protein OS=Oryza sativa subsp. japonica GN=OJ1029_F04.29 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.784e-19
Identity = 47/81 (58.02%), Postives = 60/81 (74.07%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFKXXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSGS 243
            PAF  +PLQ+AM KDTLER +EP F+LK +L +AYIHPVFK        ++S E + + V+V TKRQSRRNTP PSK++GS
Sbjct:  674 PAFVEYPLQDAMRKDTLERAREPGFDLKGYLMNAYIHPVFKGDEDDEKFSISDEPEAEQVLVATKRQSRRNTPVPSKYNGS 754

[Close]

BLAST of FG529292 vs. TrEMBL
Match: B8A9P0_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_02298 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.784e-19
Identity = 47/81 (58.02%), Postives = 60/81 (74.07%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFKXXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSGS 243
            PAF  +PLQ+AM KDTLER +EP F+LK +L +AYIHPVFK        ++S E + + V+V TKRQSRRNTP PSK++GS
Sbjct:  674 PAFVEYPLQDAMRKDTLERAREPGFDLKGYLMNAYIHPVFKGDEDDEKFSISDEPEAEQVLVATKRQSRRNTPVPSKYNGS 754

[Close]

BLAST of FG529292 vs. TAIR peptide
Match: AT4G04340.1 (| Symbols: | ERD (early-responsive to dehydration stress) family protein | chr4:2123235-2126624 FORWARD LENGTH=772)

HSP 1 Score: 99.3673 bits (246), Expect = 4.968e-22
Identity = 48/80 (60.00%), Postives = 58/80 (72.50%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFKXXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSG 240
            PAF  +PLQEAM+KDTLER +EPN NLK +LQ AYIHPVFK         M  + + + ++V TKRQSRRNTPAPS+ SG
Sbjct:  679 PAFVRYPLQEAMMKDTLERAREPNLNLKGYLQDAYIHPVFKGGDNDDDGDMIGKLENEVIIVPTKRQSRRNTPAPSRISG 758

[Close]

BLAST of FG529292 vs. TAIR peptide
Match: AT4G04340.3 (| Symbols: | ERD (early-responsive to dehydration stress) family protein | chr4:2123235-2126624 FORWARD LENGTH=772)

HSP 1 Score: 99.3673 bits (246), Expect = 4.968e-22
Identity = 48/80 (60.00%), Postives = 58/80 (72.50%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFKXXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSG 240
            PAF  +PLQEAM+KDTLER +EPN NLK +LQ AYIHPVFK         M  + + + ++V TKRQSRRNTPAPS+ SG
Sbjct:  679 PAFVRYPLQEAMMKDTLERAREPNLNLKGYLQDAYIHPVFKGGDNDDDGDMIGKLENEVIIVPTKRQSRRNTPAPSRISG 758

[Close]

BLAST of FG529292 vs. TAIR peptide
Match: AT4G04340.2 (| Symbols: | ERD (early-responsive to dehydration stress) family protein | chr4:2123235-2126624 FORWARD LENGTH=772)

HSP 1 Score: 99.3673 bits (246), Expect = 4.968e-22
Identity = 48/80 (60.00%), Postives = 58/80 (72.50%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFKXXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSG 240
            PAF  +PLQEAM+KDTLER +EPN NLK +LQ AYIHPVFK         M  + + + ++V TKRQSRRNTPAPS+ SG
Sbjct:  679 PAFVRYPLQEAMMKDTLERAREPNLNLKGYLQDAYIHPVFKGGDNDDDGDMIGKLENEVIIVPTKRQSRRNTPAPSRISG 758

[Close]

BLAST of FG529292 vs. TAIR peptide
Match: AT4G22120.6 (| Symbols: | ERD (early-responsive to dehydration stress) family protein | chr4:11715976-11719144 REVERSE LENGTH=771)

HSP 1 Score: 90.5077 bits (223), Expect = 2.308e-19
Identity = 45/81 (55.56%), Postives = 58/81 (71.60%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFK-XXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSG 240
            PAF  +PLQEAM+KDTLE  +EPN NLK +LQ+AY+HPVFK             +++++ ++V TKRQSRRNTPAPS  SG
Sbjct:  678 PAFIRYPLQEAMMKDTLETAREPNLNLKGYLQNAYVHPVFKGDEDDYDIDDKLGKFEDEAIIVPTKRQSRRNTPAPSIISG 758

[Close]

BLAST of FG529292 vs. TAIR peptide
Match: AT4G22120.5 (| Symbols: | ERD (early-responsive to dehydration stress) family protein | chr4:11715976-11719144 REVERSE LENGTH=771)

HSP 1 Score: 90.5077 bits (223), Expect = 2.308e-19
Identity = 45/81 (55.56%), Postives = 58/81 (71.60%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFK-XXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSG 240
            PAF  +PLQEAM+KDTLE  +EPN NLK +LQ+AY+HPVFK             +++++ ++V TKRQSRRNTPAPS  SG
Sbjct:  678 PAFIRYPLQEAMMKDTLETAREPNLNLKGYLQNAYVHPVFKGDEDDYDIDDKLGKFEDEAIIVPTKRQSRRNTPAPSIISG 758

[Close]

BLAST of FG529292 vs. TAIR peptide
Match: AT4G22120.4 (| Symbols: | ERD (early-responsive to dehydration stress) family protein | chr4:11715976-11719144 REVERSE LENGTH=771)

HSP 1 Score: 90.5077 bits (223), Expect = 2.308e-19
Identity = 45/81 (55.56%), Postives = 58/81 (71.60%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFK-XXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSG 240
            PAF  +PLQEAM+KDTLE  +EPN NLK +LQ+AY+HPVFK             +++++ ++V TKRQSRRNTPAPS  SG
Sbjct:  678 PAFIRYPLQEAMMKDTLETAREPNLNLKGYLQNAYVHPVFKGDEDDYDIDDKLGKFEDEAIIVPTKRQSRRNTPAPSIISG 758

[Close]

BLAST of FG529292 vs. TAIR peptide
Match: AT4G22120.3 (| Symbols: | ERD (early-responsive to dehydration stress) family protein | chr4:11715976-11719144 REVERSE LENGTH=771)

HSP 1 Score: 90.5077 bits (223), Expect = 2.308e-19
Identity = 45/81 (55.56%), Postives = 58/81 (71.60%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFK-XXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSG 240
            PAF  +PLQEAM+KDTLE  +EPN NLK +LQ+AY+HPVFK             +++++ ++V TKRQSRRNTPAPS  SG
Sbjct:  678 PAFIRYPLQEAMMKDTLETAREPNLNLKGYLQNAYVHPVFKGDEDDYDIDDKLGKFEDEAIIVPTKRQSRRNTPAPSIISG 758

[Close]

BLAST of FG529292 vs. TAIR peptide
Match: AT4G22120.2 (| Symbols: | ERD (early-responsive to dehydration stress) family protein | chr4:11715976-11719144 REVERSE LENGTH=771)

HSP 1 Score: 90.5077 bits (223), Expect = 2.308e-19
Identity = 45/81 (55.56%), Postives = 58/81 (71.60%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFK-XXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSG 240
            PAF  +PLQEAM+KDTLE  +EPN NLK +LQ+AY+HPVFK             +++++ ++V TKRQSRRNTPAPS  SG
Sbjct:  678 PAFIRYPLQEAMMKDTLETAREPNLNLKGYLQNAYVHPVFKGDEDDYDIDDKLGKFEDEAIIVPTKRQSRRNTPAPSIISG 758

[Close]

BLAST of FG529292 vs. TAIR peptide
Match: AT4G22120.1 (| Symbols: | ERD (early-responsive to dehydration stress) family protein | chr4:11715976-11719144 REVERSE LENGTH=771)

HSP 1 Score: 90.5077 bits (223), Expect = 2.308e-19
Identity = 45/81 (55.56%), Postives = 58/81 (71.60%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFK-XXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHSG 240
            PAF  +PLQEAM+KDTLE  +EPN NLK +LQ+AY+HPVFK             +++++ ++V TKRQSRRNTPAPS  SG
Sbjct:  678 PAFIRYPLQEAMMKDTLETAREPNLNLKGYLQNAYVHPVFKGDEDDYDIDDKLGKFEDEAIIVPTKRQSRRNTPAPSIISG 758

[Close]

BLAST of FG529292 vs. TAIR peptide
Match: AT1G11960.1 (| Symbols: | ERD (early-responsive to dehydration stress) family protein | chr1:4039871-4043143 REVERSE LENGTH=771)

HSP 1 Score: 86.6557 bits (213), Expect = 3.333e-18
Identity = 48/85 (56.47%), Postives = 54/85 (63.53%), Query Frame = 1

Query:    1 PAFTTHPLQEAMVKDTLERTKEPNFNLKEFLQSAYIHPVFK------XXXXXXXXAMSQEYKEDPVVVQTKRQSRRNTPAPSKHS 237
            PAF  HPL+EAMVKDTLER +EPNFNLK +LQ AYIHPVFK                 ++  E+ V V TKRQSR NTPA S  S
Sbjct:  677 PAFLRHPLKEAMVKDTLERAREPNFNLKPYLQKAYIHPVFKDNDYEDSRFDEISGYCIEDSDEECVTVPTKRQSRINTPAVSHAS 761

The following BLAST results are available for this feature:

BLAST of FG529292 vs. TrEMBL
BLAST of FG529292 vs. TAIR peptide

BLAST of FG529292 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B0BLA8_LOTJA	1.991e-25	67.07	CM0545.360.nc protein OS=Lotus japonicus GN=CM0545...	[more]
B9IAI0_POPTR	1.427e-23	59.34	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9GTV5_POPTR	1.577e-22	60.44	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9HZA2_POPTR	4.296e-20	56.32	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PJ83_9ROSI	4.296e-20	56.32	Putative uncharacterized protein OS=Populus tricho...	[more]
Q9XEA1_ARATH	1.250e-19	60.00	Putative uncharacterized protein AT4g04340 OS=Arab...	[more]
Q93ZR7_ARATH	1.250e-19	60.00	Putative uncharacterized protein At4g04340 OS=Arab...	[more]
B9N8E7_POPTR	2.132e-19	56.67	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q5JLH8_ORYSJ	2.784e-19	58.02	Os01g0534900 protein OS=Oryza sativa subsp. japoni...	[more]
B8A9P0_ORYSI	2.784e-19	58.02	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of FG529292 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT4G04340.1	4.968e-22	60.00	\| Symbols: \| ERD (early-responsive to dehydration...	[more]
AT4G04340.3	4.968e-22	60.00	\| Symbols: \| ERD (early-responsive to dehydration...	[more]
AT4G04340.2	4.968e-22	60.00	\| Symbols: \| ERD (early-responsive to dehydration...	[more]
AT4G22120.6	2.308e-19	55.56	\| Symbols: \| ERD (early-responsive to dehydration...	[more]
AT4G22120.5	2.308e-19	55.56	\| Symbols: \| ERD (early-responsive to dehydration...	[more]
AT4G22120.4	2.308e-19	55.56	\| Symbols: \| ERD (early-responsive to dehydration...	[more]
AT4G22120.3	2.308e-19	55.56	\| Symbols: \| ERD (early-responsive to dehydration...	[more]
AT4G22120.2	2.308e-19	55.56	\| Symbols: \| ERD (early-responsive to dehydration...	[more]
AT4G22120.1	2.308e-19	55.56	\| Symbols: \| ERD (early-responsive to dehydration...	[more]
AT1G11960.1	3.333e-18	56.47	\| Symbols: \| ERD (early-responsive to dehydration...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 42..49 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG529292 ID=FG529292; Name=FG529292; organism=Pisum sativum; type=EST; length=323bp
CCTGCTTTTACTACTCATCCATTACAGGAAGCAATGGTGAAAGATACATT
GGAGCGTACGAAAGAGCCAAACTTCAATTTGAAAGAGTTCCTCCAAAGTG
CATATATCCATCCCGTTTTCAAAGATGATGATGACTCGGACAGTGATGCG
ATGAGTCAAGAGTATAAGGAAGACCCGGTAGTTGTCCAAACAAAACGCCA
GTCGCGGAGGAACACACCCGCGCCTAGCAAACACAGTGGTTCATTACTGT
CCTCGGTGCATGACACGGCTGATGTATGACGATGAGCATTAACAGCCTTA
GAGTTAATGCAGCATAGTTATAT

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