FG534540

EST Overview


Name	FG534540
Unique Name	FG534540
Type	EST
Organism	Pisum sativum (pea)
Sequence length	633

Libraries


Library Name	Type
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTs	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of FG534540 vs. TrEMBL
Match: B9HTK5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_1092152 PE=4 SV=1)

HSP 1 Score: 285.034 bits (728), Expect = 3.105e-75
Identity = 136/174 (78.16%), Postives = 152/174 (87.36%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRDTNWGGLKGGQVMAQPQPVFARIENQTEVDD 522
            NAHLANTIGNLLNRTLGLLKKNC+STL VDS  AAEGNA KD + +LV+KARIHYENL+L+SACEAVLEIGN GN+YMDE APW+ FKQGG ASE AAKDLVIILE +RI+AIALSPV PS  WRIY QLGYSK+ F+  TW  T WGGLKGGQVMAQP+PVFARIEN+TE++D
Sbjct:  414 NAHLANTIGNLLNRTLGLLKKNCQSTLAVDSAVAAEGNALKDTIEKLVEKARIHYENLSLSSACEAVLEIGNAGNAYMDENAPWTRFKQGGAASEAAAKDLVIILEAMRIVAIALSPVAPSFCWRIYEQLGYSKDHFNTVTWSGTKWGGLKGGQVMAQPKPVFARIENKTELED 587

[Close]

BLAST of FG534540 vs. TrEMBL
Match: D7TLW0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_19.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00016575001 PE=4 SV=1)

HSP 1 Score: 279.641 bits (714), Expect = 1.304e-73
Identity = 139/174 (79.89%), Postives = 152/174 (87.36%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRDTNWGGLKGGQVMAQPQPVFARIENQTEVDD 522
            NAHLANTIGNLLNRTLGLLKKNC+STL VDST AAEGNAFKD V +LV+KA+  YENL+L+SACEAVLEIGN GN Y+DERAPWSLFKQG  ASE AAKDLVIILE +RIIAIALSPVTP LS RIY QLGY+KEQF+A TW +T WGGLK GQVMAQP+PVFARIE + EV D
Sbjct:  414 NAHLANTIGNLLNRTLGLLKKNCQSTLAVDSTLAAEGNAFKDTVDKLVEKAKFQYENLSLSSACEAVLEIGNAGNLYIDERAPWSLFKQGAAASEAAAKDLVIILEAMRIIAIALSPVTPRLSLRIYEQLGYTKEQFNATTWSETEWGGLKSGQVMAQPKPVFARIEIEAEVGD 587

[Close]

BLAST of FG534540 vs. TrEMBL
Match: O23761_ARATH (MEtRS OS=Arabidopsis thaliana GN=AtcpMetRS PE=2 SV=1)

HSP 1 Score: 264.618 bits (675), Expect = 4.343e-69
Identity = 129/174 (74.14%), Postives = 149/174 (85.63%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRDTNWGGLKGGQVMAQPQPVFARIENQTEVDD 522
            NAHLANTIGNLLNRTLGLLKKNCESTLVVDST AAEG   KD V +LV+KAR +YENL+L+SACEAVLEIGN GN+YMD+RAPW LFKQGG ++E AAKDLVIILE +R+IA+ALSPV P LS R+Y+QLGYS++QF++ TW DT WGGLKGGQVM Q  PVFARIE   E ++
Sbjct:  415 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENLSLSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPVAPCLSLRVYSQLGYSEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIELNPEKEE 588

[Close]

BLAST of FG534540 vs. TrEMBL
Match: Q9M2T9_ARATH (Methionyl-tRNA synthetase (AtcpMetRS) OS=Arabidopsis thaliana GN=At3g55400 PE=4 SV=1)

HSP 1 Score: 263.848 bits (673), Expect = 7.407e-69
Identity = 129/174 (74.14%), Postives = 149/174 (85.63%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRDTNWGGLKGGQVMAQPQPVFARIENQTEVDD 522
            NAHLANTIGNLLNRTLGLLKKNCESTLVVDST AAEG   KD V +LV+KAR +YENL+L+SACEAVLEIGN GN+YMD+RAPW LFKQGG ++E AAKDLVIILE +R+IA+ALSPV P LS RIY+QLGY+++QF++ TW DT WGGLKGGQVM Q  PVFARIE   E ++
Sbjct:  415 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENLSLSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPVAPCLSLRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIELNPEKEE 588

[Close]

BLAST of FG534540 vs. TrEMBL
Match: Q9ASP8_ARATH (AT3g55400/T22E16_60 OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 263.848 bits (673), Expect = 7.407e-69
Identity = 129/174 (74.14%), Postives = 149/174 (85.63%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRDTNWGGLKGGQVMAQPQPVFARIENQTEVDD 522
            NAHLANTIGNLLNRTLGLLKKNCESTLVVDST AAEG   KD V +LV+KAR +YENL+L+SACEAVLEIGN GN+YMD+RAPW LFKQGG ++E AAKDLVIILE +R+IA+ALSPV P LS RIY+QLGY+++QF++ TW DT WGGLKGGQVM Q  PVFARIE   E ++
Sbjct:  415 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENLSLSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPVAPCLSLRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIELNPEKEE 588

[Close]

BLAST of FG534540 vs. TrEMBL
Match: D7LV39_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_906959 PE=4 SV=1)

HSP 1 Score: 259.61 bits (662), Expect = 1.397e-67
Identity = 127/174 (72.99%), Postives = 147/174 (84.48%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRDTNWGGLKGGQVMAQPQPVFARIENQTEVDD 522
            NAHLANTIGNLLNRTLGLLKKNCESTLV DST AAEG   KD V +LV+KA+ +YENL+L+SACEAVLEIGN GN+YMD+RAPW LFKQGG ++E AAKDLVIILE +R+IA+ALSP+ P LS RIY+QLGYS +QF++ TW DT WGGLKGGQVM Q  PVFARIE   E ++
Sbjct:  414 NAHLANTIGNLLNRTLGLLKKNCESTLVEDSTVAAEGVPLKDTVEKLVEKAQTNYENLSLSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPIAPCLSLRIYSQLGYSVDQFNSITWSDTKWGGLKGGQVMEQASPVFARIELNPEKEE 587

[Close]

BLAST of FG534540 vs. TrEMBL
Match: C5WTP7_SORBI (Putative uncharacterized protein Sb01g043330 OS=Sorghum bicolor GN=Sb01g043330 PE=4 SV=1)

HSP 1 Score: 257.299 bits (656), Expect = 6.933e-67
Identity = 123/173 (71.10%), Postives = 145/173 (83.82%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRDTNWGGLKGGQVMAQPQPVFARIENQTEVD 519
            NAHLANTIGNLLNRTLGLLKKNC+S L  DS  AA+G  F+DN+  LVDKA+ HYENL L+SACE VLEIGN+GN Y+DE+APWS FKQGG ++E AAKDLV+ILET+RIIAIALSP+TPSLS RIY QLG++++QF    W DT WGGLK GQVM++P+PVFARIE + E D
Sbjct:  405 NAHLANTIGNLLNRTLGLLKKNCKSKLAFDSIAAADGGLFRDNIENLVDKAKHHYENLLLSSACETVLEIGNLGNLYIDEQAPWSCFKQGGESAEKAAKDLVLILETMRIIAIALSPITPSLSLRIYTQLGFTEDQFRLLRWEDTKWGGLKAGQVMSEPKPVFARIETEAEED 577

[Close]

BLAST of FG534540 vs. TrEMBL
Match: Q8H054_ORYSJ (Putative methionyl-tRNA synthetase OS=Oryza sativa subsp. japonica GN=OSJNBa0014O06.18 PE=4 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.708e-65
Identity = 122/174 (70.11%), Postives = 145/174 (83.33%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRDTNWGGLKGGQVMAQPQPVFARIENQTEVDD 522
            NAHLANTIGNLLNRTLGLLKKNC+STL  DS  AA+G + KDNV  LVDKA+  +ENL L+SACE ++EIGN+GN Y+DE+APWS FKQGG ++E AAKDLVIILET+RIIAIALSP+TPSLS RIY QLG++++QF    W DT WGGLK GQVM +P+PVFARIE +T+  D
Sbjct:  384 NAHLANTIGNLLNRTLGLLKKNCKSTLAFDSIAAADGISLKDNVENLVDKAKDQFENLLLSSACETLMEIGNLGNLYIDEQAPWSCFKQGGESAEKAAKDLVIILETMRIIAIALSPITPSLSLRIYTQLGFTEDQFRTLRWEDTKWGGLKAGQVMMEPKPVFARIETETDEKD 557

[Close]

BLAST of FG534540 vs. TrEMBL
Match: Q10Q53_ORYSJ (Methionyl-tRNA synthetase, putative, expressed OS=Oryza sativa subsp. japonica GN=Os03g0209600 PE=2 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.708e-65
Identity = 122/174 (70.11%), Postives = 145/174 (83.33%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRDTNWGGLKGGQVMAQPQPVFARIENQTEVDD 522
            NAHLANTIGNLLNRTLGLLKKNC+STL  DS  AA+G + KDNV  LVDKA+  +ENL L+SACE ++EIGN+GN Y+DE+APWS FKQGG ++E AAKDLVIILET+RIIAIALSP+TPSLS RIY QLG++++QF    W DT WGGLK GQVM +P+PVFARIE +T+  D
Sbjct:  407 NAHLANTIGNLLNRTLGLLKKNCKSTLAFDSIAAADGISLKDNVENLVDKAKDQFENLLLSSACETLMEIGNLGNLYIDEQAPWSCFKQGGESAEKAAKDLVIILETMRIIAIALSPITPSLSLRIYTQLGFTEDQFRTLRWEDTKWGGLKAGQVMMEPKPVFARIETETDEKD 580

[Close]

BLAST of FG534540 vs. TrEMBL
Match: B8AQH8_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_10474 PE=4 SV=1)

HSP 1 Score: 252.677 bits (644), Expect = 1.708e-65
Identity = 122/174 (70.11%), Postives = 145/174 (83.33%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRDTNWGGLKGGQVMAQPQPVFARIENQTEVDD 522
            NAHLANTIGNLLNRTLGLLKKNC+STL  DS  AA+G + KDNV  LVDKA+  +ENL L+SACE ++EIGN+GN Y+DE+APWS FKQGG ++E AAKDLVIILET+RIIAIALSP+TPSLS RIY QLG++++QF    W DT WGGLK GQVM +P+PVFARIE +T+  D
Sbjct:  407 NAHLANTIGNLLNRTLGLLKKNCKSTLAFDSIAAADGISLKDNVENLVDKAKDQFENLLLSSACETLMEIGNLGNLYIDEQAPWSCFKQGGESAEKAAKDLVIILETMRIIAIALSPITPSLSLRIYTQLGFTEDQFRTLRWEDTKWGGLKAGQVMMEPKPVFARIETETDEKD 580

[Close]

BLAST of FG534540 vs. SwissProt
Match: SYM_SYNY3 (Methionyl-tRNA synthetase OS=Synechocystis sp. (strain PCC 6803) GN=metG PE=3 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 7.055e-27
Identity = 73/184 (39.67%), Postives = 101/184 (54.89%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCEST-LVVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKD-LVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRD-----------TNWGGLKGGQVMAQPQPVFARIENQTE 513
            NA LAN +GNLLNRTLG++KK C+     V +T  A  N  K     L ++    YE L+ T ACEA+  +   GN Y+D+ APW LFKQG   S+   +D L  +LE++R+ A  LSP+ P LS +IY QLG++   +D   WR             +WG L   Q +   QP+F ++E   E
Sbjct:  354 NADLANDLGNLLNRTLGMVKKYCQGQGPQVMATDLAPDNPLKALGSHLGEEVSSAYERLSFTDACEAIFTLVRAGNKYIDDMAPWKLFKQG---SQKEVEDVLYSVLESIRLSAYLLSPIVPRLSTKIYQQLGFT---WDFDQWRSPLEQAEEFNRHQSWGQLGANQNLPPAQPIFTKLELPAE 531

[Close]

BLAST of FG534540 vs. SwissProt
Match: SYM_ANASP (Methionyl-tRNA synthetase OS=Anabaena sp. (strain PCC 7120) GN=metG PE=3 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 1.019e-25
Identity = 72/177 (40.68%), Postives = 95/177 (53.67%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCES-TLVVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYS---KEQFDAATWR-DTNWGGLKGGQVMAQPQPVFARIENQTEV 516
            NA LAN +GNLLNRTL ++KK C +  L + +      N  K   V L  K +  YE LA   AC  VL +    N ++D++APWSL+KQ     +     L  +LE+VR+ A  LSPV P++S  IY QLG+     EQ D   +     WG L   Q + QPQP+F RIE    V
Sbjct:  356 NADLANDLGNLLNRTLNMVKKYCANYALSITNEDIPAENTLKALGVDLGAKVKQAYEVLAFNQACGVVLSLVQASNKFIDDQAPWSLYKQ--ERQQEVETVLYTVLESVRLAAYLLSPVIPNISSNIYQQLGFGINFNEQTDITPFAIHAQWGLLSNKQQLGQPQPIFKRIEQTKNV 530

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BLAST of FG534540 vs. SwissProt
Match: SYM_THEEB (Methionyl-tRNA synthetase OS=Thermosynechococcus elongatus (strain BP-1) GN=metG PE=3 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 7.555e-21
Identity = 60/172 (34.88%), Postives = 85/172 (49.42%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLV-VDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYS-----KEQFDAATWRDTNWGGLKGGQVMAQPQPVFARI 498
            NA LAN +GNLLNRTL +  K  +  +  V        +  +     L       Y  LA    C+  L +   GN ++DE APW  ++ G TA+   A+ L  +LE+VR++A  L+P+ P LS  IY QLGYS         D    R   WG L   Q +A P+P+F ++
Sbjct:  350 NADLANDLGNLLNRTLKMAWKYTDGKVPNVQGAAIPREHPLRQLAEHLSQTYGQGYRQLAFHEVCQQALTLARAGNKFLDEEAPWKRYRAGETAA--VAEILYCVLESVRLVAYVLAPIIPQLSEAIYGQLGYSIRFNGSIAPDLLGDRQAQWGVLPASQPLANPEPIFQKL 519

[Close]

BLAST of FG534540 vs. SwissProt
Match: SYM_FUSNN (Methionyl-tRNA synthetase OS=Fusobacterium nucleatum subsp. nucleatum GN=metG PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 6.195e-15
Identity = 57/174 (32.76%), Postives = 87/174 (50.00%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNAFK---DNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRDTNWGGLKGGQVMAQPQPVFARIENQTE 513
            N+ LAN +GNLLNRTLG+ KK    T+V  ST     +  K   ++VV+ V+K    +E    + A E + +  +  N Y+DE  PW+L K     +  AA  + I+ E +  IA  ++P  P  + +I  QLG  K+  +        W   K G  + +  P+F RIE + E
Sbjct:  347 NSDLANDLGNLLNRTLGMYKKYFNGTIVSSSTVEPIDDEIKSMFNDVVKDVEKYMYLFE---FSRALETIWKFISRLNKYIDETMPWTLAKNEAKKARLAAV-MNILCEGLYKIAFLIAPYMPESAQKISNQLGIDKDITNLKFDDIKEWSIFKEGHKLGEASPIFPRIEIEKE 516

[Close]

BLAST of FG534540 vs. SwissProt
Match: SYM_CHLFF (Methionyl-tRNA synthetase OS=Chlamydophila felis (strain Fe/C-56) GN=metG PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 3.075e-14
Identity = 41/131 (31.30%), Postives = 67/131 (51.15%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNA-FKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQL 390
            N+ L    GN +NR L   +KN    LV   T   + +  F D+  R+V +AR HY   +L  AC A++E+  +GN Y++++APW L K+G   +      L       +++A+   P+ P  +W I+  L
Sbjct:  379 NSELVGKFGNFINRVLAFAEKNGFKELVYSETLLEDKDKKFLDDAQRIVQEAREHYSKYSLRKACSAIMELAALGNIYVNDQAPWKLLKEG--LAHRVEAVLFCACYCQKLLALISYPILPGTAWEIWNML 507

[Close]

BLAST of FG534540 vs. SwissProt
Match: SYM_PYRAR (Methionyl-tRNA synthetase OS=Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321) GN=metG PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.168e-13
Identity = 49/169 (28.99%), Postives = 77/169 (45.56%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRDTNWGGLKGGQVMAQPQPVFARIENQ 507
            N  L + +GN  NR L  +K      +      + E   F   V +L  KA  HY+ + L  A   V+EI   GN Y++ RAPW L K+    +E A   L     +++ +A  L+PV P  +  ++A +G S       TW +       G Q + + +P+F +I  Q
Sbjct:  382 NKVLNDDVGNYANRVLSFIKSRMGGAVPPPGKPSPEDEEFISKVAQLFQKAEAHYDAIELKEAVHTVVEIAREGNKYLNARAPWELAKKD---AEAANAVLYRAFWSLKYLAAGLAPVVPRSAETLWAMMGIS----TPLTWEEAKKPPTPGAQ-LGEVKPLFRKITEQ 542

[Close]

BLAST of FG534540 vs. SwissProt
Match: SYM_CHLCV (Methionyl-tRNA synthetase OS=Chlamydophila caviae GN=metG PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 4.440e-13
Identity = 38/130 (29.23%), Postives = 63/130 (48.46%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQL 390
            N+ L    GN +NR L   +KN    L        +   F D+  R+V +A+ +Y   +L  AC A++E+  +GN Y +++APW L K+G      A   L       +++A+   P+ P  +W I+  L
Sbjct:  379 NSELVGKFGNFINRVLAFAEKNGFRELTCPEALEDKDRQFLDDCQRIVQEAQAYYSKYSLRKACSAIMELAALGNVYFNDQAPWKLLKEGFAHRVEAI--LFCACYCQKLLALIAYPILPGTAWEIWNML 506

[Close]

BLAST of FG534540 vs. SwissProt
Match: SYM_SULAC (Methionyl-tRNA synthetase OS=Sulfolobus acidocaldarius GN=metG PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 7.573e-13
Identity = 53/175 (30.29%), Postives = 85/175 (48.57%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNAFKDNVVRLVDKARIHYENL----ALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRDTNWGGLKGGQVMAQPQPVFARIENQTE 513
            N  L + IGN  NR L ++K+  +   VV S   A  N    N++ L+ ++      L     + +  E +L++   GN Y++ RAPWSL K   T  E A   L I + ++R +AI L P+ P+ S  +Y QLG S    ++ TW       +  G  + + + +F +IE   E
Sbjct:  382 NTELNDDIGNYANRVLSMVKRYYDG--VVPSPKEAIFNDEDKNLITLIKESPKRMGELFELGKIKAGSEEILKLARSGNLYLNNRAPWSLVK---TNKEEANNVLYISVNSLRTLAIMLYPIMPTYSSNLYQQLGLS--NLESETWDSAGSLKIMPGHKIGEIRSLFKKIEMSPE 549

[Close]

BLAST of FG534540 vs. SwissProt
Match: SYM_STRP3 (Methionyl-tRNA synthetase OS=Streptococcus pyogenes serotype M3 GN=metG PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 7.573e-13
Identity = 55/175 (31.43%), Postives = 92/175 (52.57%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTL--VVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRDTNWGGLKGGQVMAQPQPVFARIENQTEVD 519
            N  LAN +GNLLNRT+ ++ K  + T+   VD+ TA + +  +    +L D  + H E +    A EAV  I    N Y+DE APW L K+ G  ++ A+  +  +  ++R++A  + P     S  I AQLG +    D +T    ++      +V+A+  P+F R++ + E+D
Sbjct:  357 NYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQVIDAQLADYHK-HMEAVDYPRALEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASV-MAHLAASLRVVAHVIQPFMMETSAAIMAQLGLAPVS-DLSTLALADFPA--NTKVVAKGTPIFPRLDMEAEID 526

[Close]

BLAST of FG534540 vs. SwissProt
Match: SYM_STRP8 (Methionyl-tRNA synthetase OS=Streptococcus pyogenes serotype M18 GN=metG PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 9.891e-13
Identity = 55/175 (31.43%), Postives = 92/175 (52.57%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTL--VVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRDTNWGGLKGGQVMAQPQPVFARIENQTEVD 519
            N  LAN +GNLLNRT+ ++ K  + T+   VD+ TA + +  +    +L D  + H E +    A EAV  I    N Y+DE APW L K+ G  ++ A+  +  +  ++R++A  + P     S  I AQLG +    D +T    ++      +V+A+  P+F R++ + E+D
Sbjct:  357 NYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQLIDAQLADYHK-HMEAVDYPRALEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASV-MAHLAASLRVVAHVIQPFMMETSAAIMAQLGLAPVS-DLSTLALADFPA--NTKVVAKGTPIFPRLDMEAEID 526

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BLAST of FG534540 vs. TAIR peptide
Match: AT3G55400.1 (| Symbols: OVA1 | methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) | chr3:20535809-20538999 REVERSE LENGTH=616)

HSP 1 Score: 263.848 bits (673), Expect = 5.311e-71
Identity = 129/174 (74.14%), Postives = 149/174 (85.63%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWRDTNWGGLKGGQVMAQPQPVFARIENQTEVDD 522
            NAHLANTIGNLLNRTLGLLKKNCESTLVVDST AAEG   KD V +LV+KAR +YENL+L+SACEAVLEIGN GN+YMD+RAPW LFKQGG ++E AAKDLVIILE +R+IA+ALSPV P LS RIY+QLGY+++QF++ TW DT WGGLKGGQVM Q  PVFARIE   E ++
Sbjct:  415 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENLSLSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPVAPCLSLRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIELNPEKEE 588

[Close]

BLAST of FG534540 vs. TAIR peptide
Match: AT3G55400.2 (| Symbols: OVA1 | methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) | chr3:20536263-20538999 REVERSE LENGTH=533)

HSP 1 Score: 161.77 bits (408), Expect = 2.843e-40
Identity = 80/101 (79.21%), Postives = 89/101 (88.12%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTTAAEGNAFKDNVVRLVDKARIHYENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDL 303
            NAHLANTIGNLLNRTLGLLKKNCESTLVVDST AAEG   KD V +LV+KAR +YENL+L+SACEAVLEIGN GN+YMD+RAPW LFKQGG ++E AAK L
Sbjct:  415 NAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENLSLSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKVL 515

[Close]

BLAST of FG534540 vs. TAIR peptide
Match: AT4G13780.1 (| Symbols: | methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS, putative | chr4:7993366-7998433 REVERSE LENGTH=797)

HSP 1 Score: 52.7582 bits (125), Expect = 1.860e-7
Identity = 44/180 (24.44%), Postives = 71/180 (39.44%), Query Frame = 1

Query:    1 NAHLANTIGNLLNRTLGLLKK--NCESTLVVDSTTAAEGNAFKDNVVRLVDKARIHY----ENLALTSACEAVLEIGNVGNSYMDERAPWSLFKQGGTASETAAKDLVIILETVRIIAIALSPVTPSLSWRIYAQLGYSKEQFDAATWR------DTNWGGLKGGQVMAQPQPVFARIEN 504
            N  L + +GN +NR L  + K  N     V+     AE ++   ++   V+K    Y    E + L    +  + I + GN Y+     W L+K+         +    +   V ++A  L P  PS S  ++ QL     QF  +  R         W  L     +  PQP+F  +EN
Sbjct:  398 NGELLSNLGNFVNRVLSFIAKPDNAGYGSVIPDAHDAESHSLTKSLAEKVEKFVAEYVEAMEKVKLKQGLKTAMLISSEGNYYLQASQFWKLYKEDKPLCAVVIRTAAGL---VHLLAQLLEPFMPSFSCEVFKQLNL-PPQFSLSDERGEVLLASRPWDILPPSHRIGTPQPLFKELEN 573

The following BLAST results are available for this feature:

BLAST of FG534540 vs. TrEMBL
BLAST of FG534540 vs. SwissProt
BLAST of FG534540 vs. TAIR peptide

BLAST of FG534540 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9HTK5_POPTR	3.105e-75	78.16	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7TLW0_VITVI	1.304e-73	79.89	Whole genome shotgun sequence of line PN40024, sca...	[more]
O23761_ARATH	4.343e-69	74.14	MEtRS OS=Arabidopsis thaliana GN=AtcpMetRS PE=2 SV...	[more]
Q9M2T9_ARATH	7.407e-69	74.14	Methionyl-tRNA synthetase (AtcpMetRS) OS=Arabidops...	[more]
Q9ASP8_ARATH	7.407e-69	74.14	AT3g55400/T22E16_60 OS=Arabidopsis thaliana PE=2 S...	[more]
D7LV39_ARALY	1.397e-67	72.99	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
C5WTP7_SORBI	6.933e-67	71.10	Putative uncharacterized protein Sb01g043330 OS=So...	[more]
Q8H054_ORYSJ	1.708e-65	70.11	Putative methionyl-tRNA synthetase OS=Oryza sativa...	[more]
Q10Q53_ORYSJ	1.708e-65	70.11	Methionyl-tRNA synthetase, putative, expressed OS=...	[more]
B8AQH8_ORYSI	1.708e-65	70.11	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of FG534540 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
SYM_SYNY3	7.055e-27	39.67	Methionyl-tRNA synthetase OS=Synechocystis sp. (st...	[more]
SYM_ANASP	1.019e-25	40.68	Methionyl-tRNA synthetase OS=Anabaena sp. (strain ...	[more]
SYM_THEEB	7.555e-21	34.88	Methionyl-tRNA synthetase OS=Thermosynechococcus e...	[more]
SYM_FUSNN	6.195e-15	32.76	Methionyl-tRNA synthetase OS=Fusobacterium nucleat...	[more]
SYM_CHLFF	3.075e-14	31.30	Methionyl-tRNA synthetase OS=Chlamydophila felis (...	[more]
SYM_PYRAR	1.168e-13	28.99	Methionyl-tRNA synthetase OS=Pyrobaculum arsenatic...	[more]
SYM_CHLCV	4.440e-13	29.23	Methionyl-tRNA synthetase OS=Chlamydophila caviae ...	[more]
SYM_SULAC	7.573e-13	30.29	Methionyl-tRNA synthetase OS=Sulfolobus acidocalda...	[more]
SYM_STRP3	7.573e-13	31.43	Methionyl-tRNA synthetase OS=Streptococcus pyogene...	[more]
SYM_STRP8	9.891e-13	31.43	Methionyl-tRNA synthetase OS=Streptococcus pyogene...	[more]

BLAST of FG534540 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT3G55400.1	5.311e-71	74.14	\| Symbols: OVA1 \| methionyl-tRNA synthetase / meth...	[more]
AT3G55400.2	2.843e-40	79.21	\| Symbols: OVA1 \| methionyl-tRNA synthetase / meth...	[more]
AT4G13780.1	1.860e-7	24.44	\| Symbols: \| methionine--tRNA ligase, putative / ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009080	Aminoacyl-tRNA synthetase, class 1a, anticodon-binding	SUPERFAMILY	47323	Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases	coord: 4..166 score: 9.3
None	No IPR available	GENE3D	1.10.730.10	no description	coord: 1..166 score: 4.8
None	No IPR available	PANTHER	PTHR11946	ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHIONYL-TRNA SYNTHETASES	coord: 1..171 score: 3.8
None	No IPR available	PANTHER	PTHR11946:SF1	METHIONYL-TRNA SYNTHETASE	coord: 1..171 score: 3.8
None	No IPR available	SEG	seg	seg	coord: 175..191 score
None	No IPR available	SEG	seg	seg	coord: 33..44 score
None	No IPR available	SEG	seg	seg	coord: 4..13 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FG534540 ID=FG534540; Name=FG534540; organism=Pisum sativum; type=EST; length=633bp
AATGCACATCTTGCCAATACAATTGGAAATCTTCTTAATCGTACTCTAGG
ACTTCTGAAAAAGAACTGTGAATCAACTCTGGTAGTGGATTCCACTACGG
CTGCAGAAGGAAACGCCTTCAAGGACAATGTAGTGAGACTGGTTGATAAA
GCTCGTATTCACTATGAAAATCTCGCATTGACATCAGCTTGTGAGGCTGT
TCTGGAGATTGGTAATGTTGGTAATTCCTATATGGATGAGCGTGCTCCTT
GGTCTCTCTTTAAACAAGGAGGCACTGCTTCTGAAACGGCCGCCAAGGAC
CTTGTAATTATATTAGAGACTGTGAGAATTATCGCCATTGCACTATCTCC
CGTTACTCCAAGCTTGAGTTGGAGAATATATGCACAACTTGGCTATTCCA
AGGAACAATTTGATGCGGCAACCTGGAGAGACACCAATTGGGGTGGACTT
AAGGGAGGTCAAGTTATGGCTCAACCTCAACCAGTGTTTGCTAGGATTGA
AAACCAAACTGAAGTGGATGACAAACCGGTTGCAGTGAGCAAAAAGTCTC
CGAAAAGTAAAGGGAAGTTGAAACAAGCTCAAGAAGTTACTGGTGCTTAG
ATGCTTTACCCTTTTCTGAATTATTTTTGTTTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0000166	nucleotide binding
GO:0004812	aminoacyl-tRNA ligase activity
GO:0005524	ATP binding

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation
GO:0006418	tRNA aminoacylation for protein translation

Vocabulary: **INTERPRO**
Term	Definition
IPR009080	tRNAsynth_Ia_anticodon-bd

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