AJ308146

EST Overview
NameAJ308146
Unique NameAJ308146
TypeEST
OrganismPisum sativum (pea)
Sequence length259
Libraries
Library NameType
LIBEST_009888 SSHcdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v2Pisum sativum unigene v2EST
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of AJ308146 vs. TrEMBL
Match: Q2PP76_MEDTR (Lectin-like protein OS=Medicago truncatula GN=Lec7 PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.916e-8
Identity = 28/31 (90.32%), Postives = 29/31 (93.55%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSSF 94
            LPEW RIGFSGATGQLVELHKILSW+F SSF
Sbjct:  236 LPEWGRIGFSGATGQLVELHKILSWTFKSSF 266
[Close]
BLAST of AJ308146 vs. TrEMBL
Match: Q2PP76_MEDTR (Lectin-like protein OS=Medicago truncatula GN=Lec7 PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 3.646e-8
Identity = 28/31 (90.32%), Postives = 29/31 (93.55%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSSF 94
            LPEW RIGFSGATGQLVELHKILSW+F SSF
Sbjct:  236 LPEWGRIGFSGATGQLVELHKILSWTFKSSF 266
[Close]
BLAST of AJ308146 vs. Lotus protein
Match: chr2.CM0177.180.r2.m (- phase: 0 /pseudo/partial)

HSP 1 Score: 57.7658 bits (138), Expect = 1.122e-9
Identity = 25/30 (83.33%), Postives = 27/30 (90.00%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSS 91
            LPEWVR+GFSGATGQLVE HKI SW+F SS
Sbjct:  255 LPEWVRVGFSGATGQLVEQHKIFSWNFSSS 284
[Close]
BLAST of AJ308146 vs. Lotus protein
Match: chr2.CM0177.210.r2.m (- phase: 0 )

HSP 1 Score: 55.0694 bits (131), Expect = 7.272e-9
Identity = 24/31 (77.42%), Postives = 26/31 (83.87%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSSF 94
            LPEWVR+GFSGATGQLVE H+I  W F SSF
Sbjct:  234 LPEWVRVGFSGATGQLVEQHRIHVWDFKSSF 264
[Close]
BLAST of AJ308146 vs. Lotus protein
Match: chr2.CM0177.200.r2.m (- phase: 2 /partial)

HSP 1 Score: 53.9138 bits (128), Expect = 1.620e-8
Identity = 23/31 (74.19%), Postives = 26/31 (83.87%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSSF 94
            LPEWVR+GFSGATG LVE H+I +W F SSF
Sbjct:  243 LPEWVRVGFSGATGLLVENHRIYAWDFKSSF 273
[Close]
BLAST of AJ308146 vs. Lotus protein
Match: LjSGA_017217.1 (+ phase: 0 )

HSP 1 Score: 51.2174 bits (121), Expect = 1.050e-7
Identity = 23/30 (76.67%), Postives = 25/30 (83.33%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSS 91
            LPEWV +GFSGAT +LVE H ILSWSF SS
Sbjct:  173 LPEWVSVGFSGATRRLVEEHNILSWSFSSS 202
[Close]
BLAST of AJ308146 vs. Lotus protein
Match: LjSGA_016434.1 (+ phase: 0 /partial)

HSP 1 Score: 50.0618 bits (118), Expect = 2.339e-7
Identity = 26/44 (59.09%), Postives = 29/44 (65.91%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSSFY*YGWL*GNKTSV 133
            LPE+VRIGFS ATGQ +ELH I SWSF SS    G    NK  +
Sbjct:  227 LPEFVRIGFSAATGQWIELHNIRSWSFNSSLDNNGGGKNNKVEI 270
[Close]
BLAST of AJ308146 vs. Lotus protein
Match: chr2.CM0177.220.r2.m (- phase: 0 )

HSP 1 Score: 49.6766 bits (117), Expect = 3.055e-7
Identity = 20/27 (74.07%), Postives = 23/27 (85.19%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSF 82
            LPEWVR+GFS +TG+LVE H ILSW F
Sbjct:  261 LPEWVRVGFSASTGELVETHDILSWGF 287
[Close]
BLAST of AJ308146 vs. Lotus protein
Match: chr2.CM0177.190.r2.m (- phase: 0 )

HSP 1 Score: 48.1358 bits (113), Expect = 8.890e-7
Identity = 20/27 (74.07%), Postives = 22/27 (81.48%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSF 82
            LPEWVR+GFSGATG LVE H I +W F
Sbjct:  261 LPEWVRVGFSGATGLLVEQHTIYAWDF 287
[Close]
BLAST of AJ308146 vs. Soybean peptides
Match: Glyma08g37340.1|PACid:16273058 ()

HSP 1 Score: 54.6842 bits (130), Expect = 2.174e-8
Identity = 24/29 (82.76%), Postives = 25/29 (86.21%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMS 88
            LPEWVRIGFSGATG +VE H ILSWSF S
Sbjct:  251 LPEWVRIGFSGATGDMVETHDILSWSFTS 279
[Close]
BLAST of AJ308146 vs. Soybean peptides
Match: Glyma18g27690.1|PACid:16309368 ()

HSP 1 Score: 54.299 bits (129), Expect = 2.840e-8
Identity = 22/27 (81.48%), Postives = 25/27 (92.59%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSF 82
            LPEWV +GFSGATGQLVE+HKI SW+F
Sbjct:  234 LPEWVTVGFSGATGQLVEIHKIFSWTF 260
[Close]
BLAST of AJ308146 vs. Soybean peptides
Match: Glyma08g37320.1|PACid:16273056 ()

HSP 1 Score: 53.9138 bits (128), Expect = 3.708e-8
Identity = 24/29 (82.76%), Postives = 25/29 (86.21%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMS 88
            LPEWVRIGFSGATG+ VE H ILSWSF S
Sbjct:  247 LPEWVRIGFSGATGEKVETHDILSWSFTS 275
[Close]
BLAST of AJ308146 vs. Soybean peptides
Match: Glyma08g37330.1|PACid:16273057 ()

HSP 1 Score: 50.0618 bits (118), Expect = 5.355e-7
Identity = 22/31 (70.97%), Postives = 27/31 (87.10%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSSF 94
            LPE+V +GFSGATG L+E H+ILSW+F SSF
Sbjct:  225 LPEFVSVGFSGATGVLIEDHEILSWTFSSSF 255
[Close]
BLAST of AJ308146 vs. Soybean peptides
Match: Glyma18g27290.1|PACid:16309366 ()

HSP 1 Score: 49.6766 bits (117), Expect = 6.994e-7
Identity = 22/30 (73.33%), Postives = 25/30 (83.33%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSS 91
            LPE+VRIGFS ATG  +E+H ILSWSF SS
Sbjct:  208 LPEFVRIGFSAATGSWIEIHNILSWSFSSS 237
[Close]
BLAST of AJ308146 vs. Soybean peptides
Match: Glyma09g33440.1|PACid:16277047 ()

HSP 1 Score: 49.2914 bits (116), Expect = 9.134e-7
Identity = 23/29 (79.31%), Postives = 23/29 (79.31%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMS 88
            L EWV IGFSGATG LVE H ILSWSF S
Sbjct:  241 LSEWVLIGFSGATGDLVETHDILSWSFNS 269
[Close]
BLAST of AJ308146 vs. Medicago proteins
Match: IMGA|Medtr8g068040.1 (Lectin alpha chain (AHRD V1 *-*- P81637); contains Interpro domain(s) IPR016363 L-type lectin, plant chr08_pseudomolecule_IMGAG_V3.5 17436664-17437452 H EGN_Mt100125 20100825)

HSP 1 Score: 64.3142 bits (155), Expect = 1.717e-11
Identity = 29/31 (93.55%), Postives = 30/31 (96.77%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSSF 94
            LPEWVRIGFSGATGQLVELHKILSW+F SSF
Sbjct:  231 LPEWVRIGFSGATGQLVELHKILSWTFKSSF 261
[Close]
BLAST of AJ308146 vs. Medicago proteins
Match: IMGA|Medtr8g068030.1 (Lectin alpha chain (AHRD V1 *-*- P81637); contains Interpro domain(s) IPR016363 L-type lectin, plant chr08_pseudomolecule_IMGAG_V3.5 17433416-17434441 E EGN_Mt100125 20100825)

HSP 1 Score: 64.3142 bits (155), Expect = 1.717e-11
Identity = 29/31 (93.55%), Postives = 30/31 (96.77%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSSF 94
            LPEWVRIGFSGATGQLVELHKILSW+F SSF
Sbjct:  235 LPEWVRIGFSGATGQLVELHKILSWTFKSSF 265
[Close]
BLAST of AJ308146 vs. Medicago proteins
Match: IMGA|Medtr8g067820.1 (Lectin alpha chain (AHRD V1 *-*- P81637); contains Interpro domain(s) IPR016363 L-type lectin, plant chr08_pseudomolecule_IMGAG_V3.5 17368523-17367735 H EGN_Mt100125 20100825)

HSP 1 Score: 64.3142 bits (155), Expect = 1.717e-11
Identity = 29/31 (93.55%), Postives = 30/31 (96.77%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSSF 94
            LPEWVRIGFSGATGQLVELHKILSW+F SSF
Sbjct:  231 LPEWVRIGFSGATGQLVELHKILSWTFKSSF 261
[Close]
BLAST of AJ308146 vs. Medicago proteins
Match: IMGA|Medtr8g067660.1 (Lectin alpha chain (AHRD V1 *-*- P81637); contains Interpro domain(s) IPR016363 L-type lectin, plant chr08_pseudomolecule_IMGAG_V3.5 17310255-17309230 E EGN_Mt100125 20100825)

HSP 1 Score: 64.3142 bits (155), Expect = 1.717e-11
Identity = 29/31 (93.55%), Postives = 30/31 (96.77%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSSF 94
            LPEWVRIGFSGATGQLVELHKILSW+F SSF
Sbjct:  235 LPEWVRIGFSGATGQLVELHKILSWTFKSSF 265
[Close]
BLAST of AJ308146 vs. Medicago proteins
Match: IMGA|Medtr5g031140.1 (Lectin alpha chain (AHRD V1 *-*- P81637); contains Interpro domain(s) IPR000985 Legume lectin, alpha chain, conserved site chr05_pseudomolecule_IMGAG_V3.5 12919376-12920377 E EGN_Mt100125 20100825)

HSP 1 Score: 53.9138 bits (128), Expect = 2.320e-8
Identity = 22/30 (73.33%), Postives = 27/30 (90.00%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSS 91
            LPEWVR+GFS ATG+LVE+H I++WSF SS
Sbjct:  256 LPEWVRVGFSAATGELVEIHDIINWSFESS 285
[Close]
BLAST of AJ308146 vs. Medicago proteins
Match: IMGA|Medtr8g067990.1 (Lectin-like protein (AHRD V1 ***- Q2PP76_MEDTR); contains Interpro domain(s) IPR001220 Legume lectin, beta chain chr08_pseudomolecule_IMGAG_V3.5 17419879-17420713 E EGN_Mt100125 20100825)

HSP 1 Score: 52.7582 bits (125), Expect = 5.168e-8
Identity = 23/34 (67.65%), Postives = 27/34 (79.41%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSSFY*Y 103
            LP+W+ +GFSGATG LVE HKILSW+F S  Y Y
Sbjct:  229 LPDWIIVGFSGATGGLVETHKILSWTFSSYKYSY 262
[Close]
BLAST of AJ308146 vs. Medicago proteins
Match: IMGA|Medtr8g067880.1 (Lectin-like protein (AHRD V1 ***- Q2PP76_MEDTR); contains Interpro domain(s) IPR001220 Legume lectin, beta chain chr08_pseudomolecule_IMGAG_V3.5 17384949-17385783 E EGN_Mt100125 20100825)

HSP 1 Score: 52.7582 bits (125), Expect = 5.168e-8
Identity = 23/34 (67.65%), Postives = 27/34 (79.41%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSSFY*Y 103
            LP+W+ +GFSGATG LVE HKILSW+F S  Y Y
Sbjct:  229 LPDWIIVGFSGATGGLVETHKILSWTFSSYKYSY 262
[Close]
BLAST of AJ308146 vs. Medicago proteins
Match: IMGA|Medtr8g067980.1 (Lectin (AHRD V1 ***- A9YWS5_MEDTR); contains Interpro domain(s) IPR016363 L-type lectin, plant chr08_pseudomolecule_IMGAG_V3.5 17415572-17416354 H EGN_Mt100125 20100825)

HSP 1 Score: 51.9878 bits (123), Expect = 8.816e-8
Identity = 23/30 (76.67%), Postives = 25/30 (83.33%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSS 91
            LPEWV +GFSGATG  VE HKILSW+F SS
Sbjct:  229 LPEWVIVGFSGATGGFVETHKILSWNFNSS 258
[Close]
BLAST of AJ308146 vs. Medicago proteins
Match: IMGA|Medtr8g067810.1 (Lectin (AHRD V1 ***- A9YWS5_MEDTR); contains Interpro domain(s) IPR016363 L-type lectin, plant chr08_pseudomolecule_IMGAG_V3.5 17363350-17364132 H EGN_Mt100125 20100825)

HSP 1 Score: 51.9878 bits (123), Expect = 8.816e-8
Identity = 23/30 (76.67%), Postives = 25/30 (83.33%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSS 91
            LPEWV +GFSGATG  VE HKILSW+F SS
Sbjct:  229 LPEWVIVGFSGATGGFVETHKILSWNFNSS 258
[Close]
BLAST of AJ308146 vs. Medicago proteins
Match: IMGA|Medtr8g068050.1 (Lectin receptor-like kinase Tg-20 (Fragment) (AHRD V1 *-*- A6YFC5_MUSAC); contains Interpro domain(s) IPR002290 Serine/threonine protein kinase chr08_pseudomolecule_IMGAG_V3.5 17443088-17440923 E EGN_Mt100125 20100825)

HSP 1 Score: 50.447 bits (119), Expect = 2.565e-7
Identity = 22/30 (73.33%), Postives = 26/30 (86.67%), Query Frame = 2 
Query:    2 LPEWVRIGFSGATGQLVELHKILSWSFMSS 91
            LPE+VRIGFS ATGQ +E+H IL+WSF SS
Sbjct:  243 LPEYVRIGFSAATGQWIEIHNILTWSFNSS 272
The following BLAST results are available for this feature:
BLAST of AJ308146 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 1
Match NameE-valueIdentityDescription
Q2PP76_MEDTR2.916e-890.32Lectin-like protein OS=Medicago truncatula GN=Lec7... [more]
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BLAST of AJ308146 vs. TrEMBL
Analysis Date: 2011-04-27 (Homology Analysis: Pisum sativum unigene v2 vs Trembl)
Total hits: 1
Match NameE-valueIdentityDescription
Q2PP76_MEDTR3.646e-890.32Lectin-like protein OS=Medicago truncatula GN=Lec7... [more]
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BLAST of AJ308146 vs. Lotus protein
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins)
Total hits: 7
Match NameE-valueIdentityDescription
chr2.CM0177.180.r2.m1.122e-983.33- phase: 0 /pseudo/partial[more]
chr2.CM0177.210.r2.m7.272e-977.42- phase: 0 [more]
chr2.CM0177.200.r2.m1.620e-874.19- phase: 2 /partial[more]
LjSGA_017217.11.050e-776.67+ phase: 0 [more]
LjSGA_016434.12.339e-759.09+ phase: 0 /partial[more]
chr2.CM0177.220.r2.m3.055e-774.07- phase: 0 [more]
chr2.CM0177.190.r2.m8.890e-774.07- phase: 0 [more]
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BLAST of AJ308146 vs. Soybean peptides
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides)
Total hits: 6
Match NameE-valueIdentityDescription
Glyma08g37340.1|PACid:162730582.174e-882.76[more]
Glyma18g27690.1|PACid:163093682.840e-881.48[more]
Glyma08g37320.1|PACid:162730563.708e-882.76[more]
Glyma08g37330.1|PACid:162730575.355e-770.97[more]
Glyma18g27290.1|PACid:163093666.994e-773.33[more]
Glyma09g33440.1|PACid:162770479.134e-779.31[more]
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BLAST of AJ308146 vs. Medicago proteins
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Medicago proteins)
Total hits: 10
Match NameE-valueIdentityDescription
IMGA|Medtr8g068040.11.717e-1193.55Lectin alpha chain (AHRD V1 *-*- P81637); contains... [more]
IMGA|Medtr8g068030.11.717e-1193.55Lectin alpha chain (AHRD V1 *-*- P81637); contains... [more]
IMGA|Medtr8g067820.11.717e-1193.55Lectin alpha chain (AHRD V1 *-*- P81637); contains... [more]
IMGA|Medtr8g067660.11.717e-1193.55Lectin alpha chain (AHRD V1 *-*- P81637); contains... [more]
IMGA|Medtr5g031140.12.320e-873.33Lectin alpha chain (AHRD V1 *-*- P81637); contains... [more]
IMGA|Medtr8g067990.15.168e-867.65Lectin-like protein (AHRD V1 ***- Q2PP76_MEDTR); c... [more]
IMGA|Medtr8g067880.15.168e-867.65Lectin-like protein (AHRD V1 ***- Q2PP76_MEDTR); c... [more]
IMGA|Medtr8g067980.18.816e-876.67Lectin (AHRD V1 ***- A9YWS5_MEDTR); contains Inter... [more]
IMGA|Medtr8g067810.18.816e-876.67Lectin (AHRD V1 ***- A9YWS5_MEDTR); contains Inter... [more]
IMGA|Medtr8g068050.12.565e-773.33Lectin receptor-like kinase Tg-20 (Fragment) (AHRD... [more]
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Properties
Property NameValue
Singlet in analysisPisum sativum unigene v2
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>AJ308146 ID=AJ308146; Name=AJ308146; organism=Pisum sativum; type=EST; length=259bp
GTTACCTGAATGGGTTAGAATTGGATTCTCTGGTGCCACTGGACAGTTAG
TTGAATTGCACAAGATTCTTTCTTGGAGTTTTATGTCAAGCTTCTACTAG
TATGGCTGGCTATAAGGGAACAAGACTTCTGTGTTTCATGTTGCTGATTT
GATGATTTGTGTGTACTAAACTACTACTATATAGTGCTTATTATTGTTAT
ATAGATATCGTGCAATGCAAGGTATTAAATATTAAGATATAAGAGTTGAA
TAAAAAAAA
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