GH719236

EST Overview


Name	GH719236
Unique Name	GH719236
Type	EST
Organism	Pisum sativum (pea)
Sequence length	247

Libraries


Library Name	Type
LIBEST_014003 TE	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of GH719236 vs. TrEMBL
Match: Q39409_BRACM (ATP synthase (Fragment) OS=Brassica campestris PE=2 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 3.056e-13
Identity = 36/47 (76.60%), Postives = 46/47 (97.87%), Query Frame = -1

Query:  104 KTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQIDLGDIQLA 244
            KT+LDPSLVAGFT+RYG++GSK IDMSVK++LE+IAAQ++LG+IQLA
Sbjct:   87 KTVLDPSLVAGFTIRYGDSGSKLIDMSVKKQLEDIAAQLELGEIQLA 133

[Close]

BLAST of GH719236 vs. TrEMBL
Match: B9MW43_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_740620 PE=3 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 3.056e-13
Identity = 37/47 (78.72%), Postives = 44/47 (93.62%), Query Frame = -1

Query:  104 KTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQIDLGDIQLA 244
            K ++DPSLVAGFTVRYGN+GSK IDMSVK++LEEIAAQ+DL DI+LA
Sbjct:  203 KPVIDPSLVAGFTVRYGNSGSKLIDMSVKKQLEEIAAQLDLSDIELA 249

[Close]

BLAST of GH719236 vs. TrEMBL
Match: B9IQM7_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_574320 PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 6.807e-13
Identity = 36/47 (76.60%), Postives = 44/47 (93.62%), Query Frame = -1

Query:  104 KTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQIDLGDIQLA 244
            KT++DPSLVAGFTVRYG++GSK IDMSVK++LEEI AQ+DL DI+LA
Sbjct:  205 KTVIDPSLVAGFTVRYGSSGSKMIDMSVKKQLEEITAQLDLSDIELA 251

[Close]

BLAST of GH719236 vs. TrEMBL
Match: D7LXC1_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_489817 PE=4 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 1.981e-12
Identity = 34/47 (72.34%), Postives = 45/47 (95.74%), Query Frame = -1

Query:  104 KTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQIDLGDIQLA 244
            KT++DPSLVAGFT+RYG++GSK IDMSVK++LE+IA Q++LG+IQLA
Sbjct:  186 KTVIDPSLVAGFTIRYGDSGSKLIDMSVKKQLEDIAQQLELGEIQLA 232

[Close]

BLAST of GH719236 vs. TrEMBL
Match: Q9SSS9_ARATH (H+-transporting ATP synthase-like protein OS=Arabidopsis thaliana GN=AT4g09650 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.284e-11
Identity = 33/47 (70.21%), Postives = 44/47 (93.62%), Query Frame = -1

Query:  104 KTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQIDLGDIQLA 244
            KT++D SLVAGFT+RYG +GSK IDMSVK++LE+IA+Q++LG+IQLA
Sbjct:  187 KTVIDASLVAGFTIRYGESGSKLIDMSVKKQLEDIASQLELGEIQLA 233

[Close]

BLAST of GH719236 vs. TrEMBL
Match: Q8RY87_ARATH (Putative H+-transporting ATP synthase (Fragment) OS=Arabidopsis thaliana GN=At4g09650 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.284e-11
Identity = 33/47 (70.21%), Postives = 44/47 (93.62%), Query Frame = -1

Query:  104 KTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQIDLGDIQLA 244
            KT++D SLVAGFT+RYG +GSK IDMSVK++LE+IA+Q++LG+IQLA
Sbjct:  203 KTVIDASLVAGFTIRYGESGSKLIDMSVKKQLEDIASQLELGEIQLA 249

[Close]

BLAST of GH719236 vs. TrEMBL
Match: C5XYR1_SORBI (Putative uncharacterized protein Sb04g027810 OS=Sorghum bicolor GN=Sb04g027810 PE=3 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 5.049e-8
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = -1

Query:  101 KTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQIDLGDIQLAV 244
            KT LDP L+AGFTV+YG  GS  IDMSV++++EEI ++ +L D+ L V
Sbjct:  200 KTQLDPELIAGFTVQYGRDGSSLIDMSVRKQIEEITSEFELPDVPLEV 247

[Close]

BLAST of GH719236 vs. TrEMBL
Match: A9NTG2_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 5.049e-8
Identity = 27/39 (69.23%), Postives = 35/39 (89.74%), Query Frame = -1

Query:  128 KTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQI 244
            KT++DPSL+AGFT+RYG TGS  IDMSVK +LE++A+QI
Sbjct:  209 KTVIDPSLIAGFTIRYGPTGSNIIDMSVKTELEKLASQI 247

[Close]

BLAST of GH719236 vs. TrEMBL
Match: Q07299_MAIZE (H(+)-transporting ATP synthase (Fragment) OS=Zea mays GN=ATP-D PE=2 SV=2)

HSP 1 Score: 59.6918 bits (143), Expect = 1.125e-7
Identity = 27/48 (56.25%), Postives = 38/48 (79.17%), Query Frame = -1

Query:  101 KTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQIDLGDIQLAV 244
            KT LDP+L+AGFTV+YG  GS  IDMSV++++EEIA++ +L  + L V
Sbjct:  138 KTQLDPALIAGFTVQYGRDGSNLIDMSVRKQIEEIASEFELPAVPLEV 185

[Close]

BLAST of GH719236 vs. TrEMBL
Match: B6TQG0_MAIZE (ATP synthase delta chain OS=Zea mays PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.125e-7
Identity = 27/48 (56.25%), Postives = 38/48 (79.17%), Query Frame = -1

Query:  101 KTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQIDLGDIQLAV 244
            KT LDP+L+AGFTV+YG  GS  IDMSV++++EEIA++ +L  + L V
Sbjct:  201 KTQLDPALIAGFTVQYGRDGSNLIDMSVRKQIEEIASEFELPAVPLEV 248

[Close]

BLAST of GH719236 vs. SwissProt
Match: ATPD_PEA (ATP synthase delta chain, chloroplastic OS=Pisum sativum GN=ATPD PE=1 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 3.979e-20
Identity = 49/49 (100.00%), Postives = 49/49 (100.00%), Query Frame = -1

Query:  101 TKTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQIDLGDIQLAV 247
            TKTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQIDLGDIQLAV
Sbjct:  203 TKTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQIDLGDIQLAV 251

[Close]

BLAST of GH719236 vs. SwissProt
Match: ATPD_TOBAC (ATP synthase delta chain, chloroplastic OS=Nicotiana tabacum GN=ATPD PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.121e-14
Identity = 36/48 (75.00%), Postives = 46/48 (95.83%), Query Frame = -1

Query:  101 KTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQIDLGDIQLAV 244
            KT++D SLVAGFT+RYGN+GSK IDMSVK++LE+IAAQ+++GDIQLAV
Sbjct:  201 KTVIDESLVAGFTIRYGNSGSKLIDMSVKKQLEDIAAQLEIGDIQLAV 248

[Close]

BLAST of GH719236 vs. SwissProt
Match: ATPD_SPIOL (ATP synthase delta chain, chloroplastic OS=Spinacia oleracea GN=ATPD PE=1 SV=2)

HSP 1 Score: 76.2554 bits (186), Expect = 5.565e-14
Identity = 34/48 (70.83%), Postives = 44/48 (91.67%), Query Frame = -1

Query:  101 KTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQIDLGDIQLAV 244
            KT++DPSLVAGFT+RYGN GSK +DMSVK++LEEIAAQ+++ D+ LAV
Sbjct:  210 KTVIDPSLVAGFTIRYGNEGSKLVDMSVKKQLEEIAAQLEMDDVTLAV 257

[Close]

BLAST of GH719236 vs. SwissProt
Match: ATPD_SORBI (ATP synthase delta chain, chloroplastic OS=Sorghum bicolor GN=ATPD PE=2 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 2.419e-9
Identity = 27/48 (56.25%), Postives = 37/48 (77.08%), Query Frame = -1

Query:  101 KTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQIDLGDIQLAV 244
            KT LDP L+AGFTV+YG  GS  IDMSV++++EEI ++ +L D+ L V
Sbjct:  200 KTQLDPELIAGFTVQYGRDGSSLIDMSVRKQIEEITSEFELPDVPLEV 247

[Close]

BLAST of GH719236 vs. TAIR peptide
Match: AT4G09650.1 (| Symbols: ATPD | ATP synthase delta-subunit gene | chr4:6100799-6101503 FORWARD LENGTH=234)

HSP 1 Score: 72.7886 bits (177), Expect = 4.980e-14
Identity = 33/47 (70.21%), Postives = 44/47 (93.62%), Query Frame = -1

Query:  104 KTLLDPSLVAGFTVRYGNTGSKFIDMSVKRKLEEIAAQIDLGDIQLA 244
            KT++D SLVAGFT+RYG +GSK IDMSVK++LE+IA+Q++LG+IQLA
Sbjct:  187 KTVIDASLVAGFTIRYGESGSKLIDMSVKKQLEDIASQLELGEIQLA 233

The following BLAST results are available for this feature:

BLAST of GH719236 vs. TrEMBL
BLAST of GH719236 vs. SwissProt
BLAST of GH719236 vs. TAIR peptide

BLAST of GH719236 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q39409_BRACM	3.056e-13	76.60	ATP synthase (Fragment) OS=Brassica campestris PE=...	[more]
B9MW43_POPTR	3.056e-13	78.72	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9IQM7_POPTR	6.807e-13	76.60	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7LXC1_ARALY	1.981e-12	72.34	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q9SSS9_ARATH	1.284e-11	70.21	H+-transporting ATP synthase-like protein OS=Arabi...	[more]
Q8RY87_ARATH	1.284e-11	70.21	Putative H+-transporting ATP synthase (Fragment) O...	[more]
C5XYR1_SORBI	5.049e-8	56.25	Putative uncharacterized protein Sb04g027810 OS=So...	[more]
A9NTG2_PICSI	5.049e-8	69.23	Putative uncharacterized protein OS=Picea sitchens...	[more]
Q07299_MAIZE	1.125e-7	56.25	H(+)-transporting ATP synthase (Fragment) OS=Zea m...	[more]
B6TQG0_MAIZE	1.125e-7	56.25	ATP synthase delta chain OS=Zea mays PE=2 SV=1	[more]

BLAST of GH719236 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 4


Match Name	E-value	Identity	Description
ATPD_PEA	3.979e-20	100.00	ATP synthase delta chain, chloroplastic OS=Pisum s...	[more]
ATPD_TOBAC	1.121e-14	75.00	ATP synthase delta chain, chloroplastic OS=Nicotia...	[more]
ATPD_SPIOL	5.565e-14	70.83	ATP synthase delta chain, chloroplastic OS=Spinaci...	[more]
ATPD_SORBI	2.419e-9	56.25	ATP synthase delta chain, chloroplastic OS=Sorghum...	[more]

BLAST of GH719236 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G09650.1	4.980e-14	70.21	\| Symbols: ATPD \| ATP synthase delta-subunit gene ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000711	ATPase, F1 complex, OSCP/delta subunit	PANTHER	PTHR11910	ATP SYNTHASE DELTA CHAIN	coord: 6..46 score: 2
IPR000711	ATPase, F1 complex, OSCP/delta subunit	PFAM	PF00213	OSCP	coord: 3..39 score: 9.8

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH719236 ID=GH719236; Name=GH719236; organism=Pisum sativum; type=EST; length=247bp
CACTTGAGGATTCATACATAGATACATAAATATACTTATAATCCAAAAAA
AAATTACAATTTGAGTAAACCAAATTGAAAGGAAGCACAAAGAATGCTTA
TACAGCAAGTTGGATATCACCCAAATCAATTTGAGCAGCAATTTCCTCGA
GTTTCCTCTTAACACTCATATCAATAAACTTAGAGCCAGTATTCCCATAC
CTGACAGTAAAACCAGCCACCAAACTTGGATCAAGAAGTGTTTTAGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005886	plasma membrane

Vocabulary: **Biological Process**
Term	Definition
GO:0015986	proton motive force-driven ATP synthesis

Vocabulary: **INTERPRO**
Term	Definition
IPR000711	ATPase_OSCP/dsu

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