GH720351

EST Overview


Name	GH720351
Unique Name	GH720351
Type	EST
Organism	Pisum sativum (pea)
Sequence length	207

Libraries


Library Name	Type
LIBEST_024270 552I	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of GH720351 vs. TrEMBL
Match: B9RGY0_RICCO (Lysosomal alpha-mannosidase, putative OS=Ricinus communis GN=RCOM_1445250 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.400e-17
Identity = 44/59 (74.58%), Postives = 48/59 (81.36%), Query Frame = -2

Query:   18 RGTVGQEIYSPLLLAFTEQDEDNWLHFQQPTFSGIDSSYSLPNNTALLTLQDFGNGKYL 194
            R TVGQEIYSP+LLAF EQD  NW++   PTFSGID SYSLPNNTALLTLQ+  NGK L
Sbjct:  856 RRTVGQEIYSPILLAFAEQDGSNWMNSHIPTFSGIDPSYSLPNNTALLTLQELENGKVL 914

[Close]

BLAST of GH720351 vs. TrEMBL
Match: D7SVH6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_68.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00032988001 PE=4 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.866e-15
Identity = 40/59 (67.80%), Postives = 46/59 (77.97%), Query Frame = -2

Query:   18 RGTVGQEIYSPLLLAFTEQDEDNWLHFQQPTFSGIDSSYSLPNNTALLTLQDFGNGKYL 194
            R T GQEIYSPLLLAF EQD +NW+    PTFSG+D SY LPNNTAL+TL++  NGK L
Sbjct:  853 RRTFGQEIYSPLLLAFAEQDGNNWMESHLPTFSGLDPSYVLPNNTALITLEELENGKLL 911

[Close]

BLAST of GH720351 vs. TrEMBL
Match: D7SVH7_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_68.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00032989001 PE=4 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.429e-15
Identity = 39/59 (66.10%), Postives = 46/59 (77.97%), Query Frame = -2

Query:   18 RGTVGQEIYSPLLLAFTEQDEDNWLHFQQPTFSGIDSSYSLPNNTALLTLQDFGNGKYL 194
            R + GQEIYSP LLAFTE+D DNW+    PTFSGID SYSLP+N AL+TLQ+  +GK L
Sbjct:  854 RRSAGQEIYSPFLLAFTEEDGDNWMSSHVPTFSGIDPSYSLPDNVALITLQELDDGKVL 912

[Close]

BLAST of GH720351 vs. TrEMBL
Match: B9GNG2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_816882 PE=4 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 7.090e-15
Identity = 41/61 (67.21%), Postives = 47/61 (77.05%), Query Frame = -2

Query:   18 RGRGTVGQEIYSPLLLAFTEQDEDNWLHFQQPTFSGIDSSYSLPNNTALLTLQDFGNGKYL 200
            R R T GQEIYSP+LLAFTEQD    ++F  PTFSGID SYSLPNN A++TLQ+  NGK L
Sbjct:  857 RWRRTFGQEIYSPVLLAFTEQDGSTEMNFPLPTFSGIDPSYSLPNNVAVITLQELENGKVL 917

[Close]

BLAST of GH720351 vs. TrEMBL
Match: A9PF27_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 7.090e-15
Identity = 41/61 (67.21%), Postives = 47/61 (77.05%), Query Frame = -2

Query:   18 RGRGTVGQEIYSPLLLAFTEQDEDNWLHFQQPTFSGIDSSYSLPNNTALLTLQDFGNGKYL 200
            R R T GQEIYSP+LLAFTEQD    ++F  PTFSGID SYSLPNN A++TLQ+  NGK L
Sbjct:  569 RWRRTFGQEIYSPVLLAFTEQDGSTEMNFPLPTFSGIDPSYSLPNNVAVITLQELENGKVL 629

[Close]

BLAST of GH720351 vs. TrEMBL
Match: B9RGY1_RICCO (Lysosomal alpha-mannosidase, putative OS=Ricinus communis GN=RCOM_1445260 PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.337e-13
Identity = 37/59 (62.71%), Postives = 45/59 (76.27%), Query Frame = -2

Query:   18 RGTVGQEIYSPLLLAFTEQDEDNWLHFQQPTFSGIDSSYSLPNNTALLTLQDFGNGKYL 194
            R + GQEIYSPLLLAF E+DEDNW+     TFSGID SY LP+N A++TLQ+  +GK L
Sbjct:  815 RRSFGQEIYSPLLLAFAEEDEDNWMKSHITTFSGIDPSYRLPDNVAVITLQELDDGKVL 873

[Close]

BLAST of GH720351 vs. TrEMBL
Match: B9IA60_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_572859 PE=4 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.746e-13
Identity = 37/59 (62.71%), Postives = 46/59 (77.97%), Query Frame = -2

Query:   18 RGTVGQEIYSPLLLAFTEQDEDNWLHFQQPTFSGIDSSYSLPNNTALLTLQDFGNGKYL 194
            R + GQEIYSPLLLAF+E+D DNW++    TFSG DSSY LP+N A+LTLQ+  +GK L
Sbjct:  847 RRSFGQEIYSPLLLAFSEEDGDNWMNSHVTTFSGFDSSYILPDNVAVLTLQELDDGKVL 905

[Close]

BLAST of GH720351 vs. TrEMBL
Match: A5AHH3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_032907 PE=4 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 2.979e-13
Identity = 36/59 (61.02%), Postives = 45/59 (76.27%), Query Frame = -2

Query:   18 RGTVGQEIYSPLLLAFTEQDEDNWLHFQQPTFSGIDSSYSLPNNTALLTLQDFGNGKYL 194
            R + GQEIYSPLLLAF+EQD DNW +   P+FSG+D SY LP+N A++TLQ+   GK L
Sbjct:  720 RRSYGQEIYSPLLLAFSEQDGDNWANSHLPSFSGMDPSYMLPDNIAMITLQELDEGKVL 778

[Close]

BLAST of GH720351 vs. TrEMBL
Match: P94078_ARATH (AT3g26720/MLJ15_12 OS=Arabidopsis thaliana GN=At3g26720 PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 5.081e-13
Identity = 36/59 (61.02%), Postives = 45/59 (76.27%), Query Frame = -2

Query:   18 RGTVGQEIYSPLLLAFTEQDEDNWLHFQQPTFSGIDSSYSLPNNTALLTLQDFGNGKYL 194
            R T GQEIYSPLL+AFTEQ+ D+W++  + TFS  + SYSLP N ALLTLQ+  NG+ L
Sbjct:  852 RRTFGQEIYSPLLIAFTEQEGDSWINSHKTTFSAFEPSYSLPKNVALLTLQELENGEVL 910

[Close]

BLAST of GH720351 vs. TrEMBL
Match: D7LR85_ARALY (Glycosyl hydrolase family 38 protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_484445 PE=4 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 5.081e-13
Identity = 37/59 (62.71%), Postives = 44/59 (74.58%), Query Frame = -2

Query:   18 RGTVGQEIYSPLLLAFTEQDEDNWLHFQQPTFSGIDSSYSLPNNTALLTLQDFGNGKYL 194
            R T GQEIYSPLLLAFTEQ+ D+W+   + TFS  + SYSLP N ALLTLQ+  NG+ L
Sbjct:  852 RRTFGQEIYSPLLLAFTEQEGDSWISSHKTTFSAFEPSYSLPKNVALLTLQELENGEVL 910

[Close]

BLAST of GH720351 vs. TAIR peptide
Match: AT3G26720.2 (| Symbols: | Glycosyl hydrolase family 38 protein | chr3:9816707-9822958 FORWARD LENGTH=943)

HSP 1 Score: 77.411 bits (189), Expect = 2.023e-15
Identity = 36/59 (61.02%), Postives = 45/59 (76.27%), Query Frame = -2

Query:   18 RGTVGQEIYSPLLLAFTEQDEDNWLHFQQPTFSGIDSSYSLPNNTALLTLQDFGNGKYL 194
            R T GQEIYSPLL+AFTEQ+ D+W++  + TFS  + SYSLP N ALLTLQ+  NG+ L
Sbjct:  852 RRTFGQEIYSPLLIAFTEQEGDSWINSHKTTFSAFEPSYSLPKNVALLTLQELENGEVL 910

[Close]

BLAST of GH720351 vs. TAIR peptide
Match: AT3G26720.1 (| Symbols: | Glycosyl hydrolase family 38 protein | chr3:9816707-9823056 FORWARD LENGTH=1019)

HSP 1 Score: 77.411 bits (189), Expect = 2.023e-15
Identity = 36/59 (61.02%), Postives = 45/59 (76.27%), Query Frame = -2

Query:   18 RGTVGQEIYSPLLLAFTEQDEDNWLHFQQPTFSGIDSSYSLPNNTALLTLQDFGNGKYL 194
            R T GQEIYSPLL+AFTEQ+ D+W++  + TFS  + SYSLP N ALLTLQ+  NG+ L
Sbjct:  852 RRTFGQEIYSPLLIAFTEQEGDSWINSHKTTFSAFEPSYSLPKNVALLTLQELENGEVL 910

[Close]

BLAST of GH720351 vs. TAIR peptide
Match: AT5G13980.3 (| Symbols: | Glycosyl hydrolase family 38 protein | chr5:4508626-4513928 FORWARD LENGTH=921)

HSP 1 Score: 72.4034 bits (176), Expect = 6.509e-14
Identity = 36/59 (61.02%), Postives = 43/59 (72.88%), Query Frame = -2

Query:   18 RGTVGQEIYSPLLLAFTEQDEDNWLHFQQPTFSGIDSSYSLPNNTALLTLQDFGNGKYL 194
            R T GQEIYSPLLLAF +QD+   + F   +FSGID SYSLP+N ALLTLQ+  +G  L
Sbjct:  855 RRTFGQEIYSPLLLAFAQQDDGKPMSFGAASFSGIDPSYSLPDNVALLTLQELDDGNVL 913

[Close]

BLAST of GH720351 vs. TAIR peptide
Match: AT5G13980.2 (| Symbols: | Glycosyl hydrolase family 38 protein | chr5:4508626-4514334 FORWARD LENGTH=1024)

HSP 1 Score: 72.4034 bits (176), Expect = 6.509e-14
Identity = 36/59 (61.02%), Postives = 43/59 (72.88%), Query Frame = -2

Query:   18 RGTVGQEIYSPLLLAFTEQDEDNWLHFQQPTFSGIDSSYSLPNNTALLTLQDFGNGKYL 194
            R T GQEIYSPLLLAF +QD+   + F   +FSGID SYSLP+N ALLTLQ+  +G  L
Sbjct:  855 RRTFGQEIYSPLLLAFAQQDDGKPMSFGAASFSGIDPSYSLPDNVALLTLQELDDGNVL 913

[Close]

BLAST of GH720351 vs. TAIR peptide
Match: AT5G13980.1 (| Symbols: | Glycosyl hydrolase family 38 protein | chr5:4508626-4514334 FORWARD LENGTH=1024)

HSP 1 Score: 72.4034 bits (176), Expect = 6.509e-14
Identity = 36/59 (61.02%), Postives = 43/59 (72.88%), Query Frame = -2

Query:   18 RGTVGQEIYSPLLLAFTEQDEDNWLHFQQPTFSGIDSSYSLPNNTALLTLQDFGNGKYL 194
            R T GQEIYSPLLLAF +QD+   + F   +FSGID SYSLP+N ALLTLQ+  +G  L
Sbjct:  855 RRTFGQEIYSPLLLAFAQQDDGKPMSFGAASFSGIDPSYSLPDNVALLTLQELDDGNVL 913

[Close]

BLAST of GH720351 vs. TAIR peptide
Match: AT5G66150.1 (| Symbols: | Glycosyl hydrolase family 38 protein | chr5:26439013-26444434 REVERSE LENGTH=1047)

HSP 1 Score: 53.5286 bits (127), Expect = 3.129e-8
Identity = 26/62 (41.94%), Postives = 36/62 (58.06%), Query Frame = -2

Query:   18 GRGRGTVGQEIYSPLLLAFTEQDEDNWLHFQQPTFSGIDSSYSLPNNTALLTLQDFGNGKYL 203
            GR R   GQEIYSPLL+AF  ++++ W          +D  Y+LP N AL+TL++   G  L
Sbjct:  880 GRWRRETGQEIYSPLLMAFAHENKEKWKASNTVKGYAMDHLYTLPQNIALITLEELDLGNVL 941

The following BLAST results are available for this feature:

BLAST of GH720351 vs. TrEMBL
BLAST of GH720351 vs. TAIR peptide

BLAST of GH720351 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9RGY0_RICCO	3.400e-17	74.58	Lysosomal alpha-mannosidase, putative OS=Ricinus c...	[more]
D7SVH6_VITVI	1.866e-15	67.80	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7SVH7_VITVI	5.429e-15	66.10	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9GNG2_POPTR	7.090e-15	67.21	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PF27_POPTR	7.090e-15	67.21	Putative uncharacterized protein OS=Populus tricho...	[more]
B9RGY1_RICCO	1.337e-13	62.71	Lysosomal alpha-mannosidase, putative OS=Ricinus c...	[more]
B9IA60_POPTR	1.746e-13	62.71	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A5AHH3_VITVI	2.979e-13	61.02	Putative uncharacterized protein OS=Vitis vinifera...	[more]
P94078_ARATH	5.081e-13	61.02	AT3g26720/MLJ15_12 OS=Arabidopsis thaliana GN=At3g...	[more]
D7LR85_ARALY	5.081e-13	62.71	Glycosyl hydrolase family 38 protein OS=Arabidopsi...	[more]

BLAST of GH720351 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT3G26720.2	2.023e-15	61.02	\| Symbols: \| Glycosyl hydrolase family 38 protein...	[more]
AT3G26720.1	2.023e-15	61.02	\| Symbols: \| Glycosyl hydrolase family 38 protein...	[more]
AT5G13980.3	6.509e-14	61.02	\| Symbols: \| Glycosyl hydrolase family 38 protein...	[more]
AT5G13980.2	6.509e-14	61.02	\| Symbols: \| Glycosyl hydrolase family 38 protein...	[more]
AT5G13980.1	6.509e-14	61.02	\| Symbols: \| Glycosyl hydrolase family 38 protein...	[more]
AT5G66150.1	3.129e-8	41.94	\| Symbols: \| Glycosyl hydrolase family 38 protein...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR11607	ALPHA-MANNOSIDASE	coord: 7..60 score: 1.7
None	No IPR available	PANTHER	PTHR11607:SF3	LYSOSOMAL ALPHA-MANNOSIDASE (MANNOSIDASE ALPHA CLASS 2B MEMBER 1)	coord: 7..60 score: 1.7

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720351 ID=GH720351; Name=GH720351; organism=Pisum sativum; type=EST; length=207bp
TTCGAGCGGCCGCCGGGCAGGTATTTTCCGTTTCCAAAATCTTGGAGAGT
TAGAAGTGCGGTGTTGTTTGGTAAACTGTAGGAAGAGTCTATGCCAGAGA
ATGTTGGTTGTTGAAAATGCAACCAATTATCCTCATCCTGCTCTGTGAAG
GCCAACAACAATGGTGAATATATTTCCTGGCCAACTGTACCTCGGCCGCG
ACCACGC

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