AM162076

EST Overview
NameAM162076
Unique NameAM162076
TypeEST
OrganismPisum sativum (pea)
Sequence length240
Libraries
Library NameType
LIBEST_019360 Pisum sativum leaf vegetativecdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of AM162076 vs. TrEMBL
Match: Q8L5Q6_CICAR (Glutathione peroxidase OS=Cicer arietinum GN=phgpx PE=2 SV=1)

HSP 1 Score: 48.9062 bits (115), Expect = 1.400e-11
Identity = 23/25 (92.00%), Postives = 25/25 (100.00%), Query Frame = 1 
Query:    1 AEKSSKSIYDFTVKDIRGNDVSLSE 75
            AE++SKSIYDFTVKDIRGNDVSLSE
Sbjct:    2 AEQASKSIYDFTVKDIRGNDVSLSE 26

HSP 2 Score: 43.8986 bits (102), Expect = 1.400e-11
Identity = 19/24 (79.17%), Postives = 21/24 (87.50%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELN 155
            G+   +VNVASQCGLTQTNYKELN
Sbjct:   29 GKVLLIVNVASQCGLTQTNYKELN 52
[Close]
BLAST of AM162076 vs. TrEMBL
Match: C6T3W7_SOYBN (Glutathione peroxidase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 44.669 bits (104), Expect = 2.465e-10
Identity = 21/24 (87.50%), Postives = 23/24 (95.83%), Query Frame = 1 
Query:    1 AEKSSKSIYDFTVKDIRGNDVSLS 72
            AE+SSKSIYDFTVKDI GNDVSL+
Sbjct:   35 AEQSSKSIYDFTVKDISGNDVSLN 58

HSP 2 Score: 43.8986 bits (102), Expect = 2.465e-10
Identity = 19/24 (79.17%), Postives = 21/24 (87.50%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELN 155
            G+   +VNVASQCGLTQTNYKELN
Sbjct:   62 GKVLLIVNVASQCGLTQTNYKELN 85
[Close]
BLAST of AM162076 vs. TrEMBL
Match: C6T020_SOYBN (Glutathione peroxidase OS=Glycine max PE=2 SV=1)

HSP 1 Score: 43.8986 bits (102), Expect = 5.443e-10
Identity = 19/24 (79.17%), Postives = 21/24 (87.50%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELN 155
            G+   +VNVASQCGLTQTNYKELN
Sbjct:   29 GKVLLIVNVASQCGLTQTNYKELN 52

HSP 2 Score: 43.5134 bits (101), Expect = 5.443e-10
Identity = 20/25 (80.00%), Postives = 23/25 (92.00%), Query Frame = 1 
Query:    1 AEKSSKSIYDFTVKDIRGNDVSLSE 75
            AE+SS SIYDFTVKDI GNDVSL++
Sbjct:    2 AEQSSNSIYDFTVKDISGNDVSLND 26
[Close]
BLAST of AM162076 vs. TrEMBL
Match: A5AU08_VITVI (Glutathione peroxidase OS=Vitis vinifera GN=VITISV_034775 PE=3 SV=1)

HSP 1 Score: 45.0542 bits (105), Expect = 5.668e-9
Identity = 21/25 (84.00%), Postives = 23/25 (92.00%), Query Frame = 1 
Query:    1 AEKSSKSIYDFTVKDIRGNDVSLSE 75
            AE + KSIYDFTVKDIRGNDVSLS+
Sbjct:    2 AEAAPKSIYDFTVKDIRGNDVSLSD 26

HSP 2 Score: 38.891 bits (89), Expect = 5.668e-9
Identity = 16/24 (66.67%), Postives = 20/24 (83.33%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELN 155
            G+   +VNVAS+CGLT +NYKELN
Sbjct:   29 GKVLLIVNVASKCGLTHSNYKELN 52
[Close]
BLAST of AM162076 vs. TrEMBL
Match: D0PWB5_THEHA (Glutathione peroxidase OS=Thellungiella halophila GN=GPX2 PE=2 SV=1)

HSP 1 Score: 45.4394 bits (106), Expect = 7.350e-9
Identity = 24/34 (70.59%), Postives = 28/34 (82.35%), Query Frame = 1 
Query:    1 AEKSSKSIYDFTVKDIRGNDVSLSESQWEGSTAL 102
            A++S KSIYDFTVKDI GNDVSL  SQ++G T L
Sbjct:    2 ADESPKSIYDFTVKDIGGNDVSL--SQFKGKTLL 33

HSP 2 Score: 38.1206 bits (87), Expect = 7.350e-9
Identity = 16/24 (66.67%), Postives = 19/24 (79.17%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELN 155
            G+   +VNVAS+CGLT  NYKELN
Sbjct:   29 GKTLLIVNVASKCGLTDANYKELN 52
[Close]
BLAST of AM162076 vs. TrEMBL
Match: B9HH74_POPTR (Glutathione peroxidase OS=Populus trichocarpa GN=PtrcGpx2_1 PE=3 SV=1)

HSP 1 Score: 44.669 bits (104), Expect = 7.355e-9
Identity = 21/25 (84.00%), Postives = 22/25 (88.00%), Query Frame = 1 
Query:    1 AEKSSKSIYDFTVKDIRGNDVSLSE 75
            AE+S KSIYDFTVKDI GND SLSE
Sbjct:    2 AEESPKSIYDFTVKDIHGNDTSLSE 26

HSP 2 Score: 38.891 bits (89), Expect = 7.355e-9
Identity = 16/24 (66.67%), Postives = 20/24 (83.33%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELN 155
            G+   +VNVAS+CGLT +NYKELN
Sbjct:   29 GKVLLIVNVASKCGLTHSNYKELN 52
[Close]
BLAST of AM162076 vs. TrEMBL
Match: Q8W259_MOMCH (Glutathione peroxidase OS=Momordica charantia GN=PHGPX PE=2 SV=1)

HSP 1 Score: 45.4394 bits (106), Expect = 1.238e-8
Identity = 24/34 (70.59%), Postives = 26/34 (76.47%), Query Frame = 1 
Query:    1 AEKSSKSIYDFTVKDIRGNDVSLSESQWEGSTAL 102
            AE+S KSIYDFTVKDIRGNDV L  SQ+ G   L
Sbjct:    2 AEESPKSIYDFTVKDIRGNDVCL--SQYSGKVLL 33

HSP 2 Score: 37.3502 bits (85), Expect = 1.238e-8
Identity = 15/24 (62.50%), Postives = 19/24 (79.17%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELN 155
            G+   +VNVAS+CG T +NYKELN
Sbjct:   29 GKVLLIVNVASKCGFTDSNYKELN 52
[Close]
BLAST of AM162076 vs. TrEMBL
Match: D7RV80_BRANA (Glutathione peroxidase 2 OS=Brassica napus GN=GPX2 PE=4 SV=1)

HSP 1 Score: 43.5134 bits (101), Expect = 2.703e-8
Identity = 23/34 (67.65%), Postives = 27/34 (79.41%), Query Frame = 1 
Query:    1 AEKSSKSIYDFTVKDIRGNDVSLSESQWEGSTAL 102
            AE+S +SIYDFTVKDI G DVSL  SQ++G T L
Sbjct:    2 AEESPQSIYDFTVKDIEGKDVSL--SQFKGKTLL 33

HSP 2 Score: 38.1206 bits (87), Expect = 2.703e-8
Identity = 16/24 (66.67%), Postives = 19/24 (79.17%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELN 155
            G+   +VNVAS+CGLT  NYKELN
Sbjct:   29 GKTLLIVNVASKCGLTDANYKELN 52
[Close]
BLAST of AM162076 vs. TrEMBL
Match: D7RV79_BRANA (Glutathione peroxidase 2 OS=Brassica napus GN=GPX2 PE=2 SV=1)

HSP 1 Score: 43.5134 bits (101), Expect = 2.703e-8
Identity = 23/34 (67.65%), Postives = 27/34 (79.41%), Query Frame = 1 
Query:    1 AEKSSKSIYDFTVKDIRGNDVSLSESQWEGSTAL 102
            AE+S +SIYDFTVKDI G DVSL  SQ++G T L
Sbjct:    2 AEESPQSIYDFTVKDIEGKDVSL--SQFKGKTLL 33

HSP 2 Score: 38.1206 bits (87), Expect = 2.703e-8
Identity = 16/24 (66.67%), Postives = 19/24 (79.17%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELN 155
            G+   +VNVAS+CGLT  NYKELN
Sbjct:   29 GKTLLIVNVASKCGLTDANYKELN 52
[Close]
BLAST of AM162076 vs. TrEMBL
Match: A9NQB1_PICSI (Glutathione peroxidase OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 40.817 bits (94), Expect = 3.463e-8
Identity = 18/24 (75.00%), Postives = 20/24 (83.33%), Query Frame = 1 
Query:    1 AEKSSKSIYDFTVKDIRGNDVSLS 72
            + K S S+YDFTVKDIRGNDV LS
Sbjct:   81 SSKQSTSVYDFTVKDIRGNDVDLS 104

HSP 2 Score: 40.4318 bits (93), Expect = 3.463e-8
Identity = 17/25 (68.00%), Postives = 20/25 (80.00%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELNE 158
            G+   +VNVASQCGLT +NY ELNE
Sbjct:  108 GKVLLIVNVASQCGLTNSNYNELNE 132
[Close]
BLAST of AM162076 vs. SwissProt
Match: GPX2_ARATH (Probable glutathione peroxidase 2 OS=Arabidopsis thaliana GN=GPX2 PE=1 SV=1)

HSP 1 Score: 44.2838 bits (103), Expect = 6.154e-10
Identity = 23/34 (67.65%), Postives = 27/34 (79.41%), Query Frame = 1 
Query:    1 AEKSSKSIYDFTVKDIRGNDVSLSESQWEGSTAL 102
            A++S KSIYDFTVKDI GNDVSL   Q++G T L
Sbjct:    2 ADESPKSIYDFTVKDIGGNDVSL--DQYKGKTLL 33

HSP 2 Score: 38.5058 bits (88), Expect = 6.154e-10
Identity = 17/24 (70.83%), Postives = 19/24 (79.17%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELN 155
            G+   VVNVAS+CGLT  NYKELN
Sbjct:   29 GKTLLVVNVASKCGLTDANYKELN 52
[Close]
BLAST of AM162076 vs. SwissProt
Match: GPX4_GOSHI (Probable phospholipid hydroperoxide glutathione peroxidase OS=Gossypium hirsutum PE=2 SV=1)

HSP 1 Score: 37.3502 bits (85), Expect = 2.435e-7
Identity = 15/29 (51.72%), Postives = 21/29 (72.41%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELNEFIRE 170
            G+   +VNVASQCGLT +NY +L E  ++
Sbjct:   31 GKVLIIVNVASQCGLTNSNYTDLTEIYKK 59

HSP 2 Score: 36.5798 bits (83), Expect = 2.435e-7
Identity = 19/26 (73.08%), Postives = 21/26 (80.77%), Query Frame = 1 
Query:    1 AEKSSK--SIYDFTVKDIRGNDVSLS 72
            A +SSK  SIYDFTVKD +GNDV LS
Sbjct:    2 ASQSSKPQSIYDFTVKDAKGNDVDLS 27
[Close]
BLAST of AM162076 vs. SwissProt
Match: GPX4_NICSY (Probable phospholipid hydroperoxide glutathione peroxidase OS=Nicotiana sylvestris PE=2 SV=1)

HSP 1 Score: 37.3502 bits (85), Expect = 2.435e-7
Identity = 15/29 (51.72%), Postives = 21/29 (72.41%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELNEFIRE 170
            G+   +VNVASQCGLT +NY +L E  ++
Sbjct:   31 GKVLIIVNVASQCGLTNSNYTDLTEIYKK 59

HSP 2 Score: 36.5798 bits (83), Expect = 2.435e-7
Identity = 19/26 (73.08%), Postives = 21/26 (80.77%), Query Frame = 1 
Query:    1 AEKSSK--SIYDFTVKDIRGNDVSLS 72
            A +SSK  SIYDFTVKD +GNDV LS
Sbjct:    2 ASQSSKPQSIYDFTVKDAKGNDVDLS 27
[Close]
BLAST of AM162076 vs. SwissProt
Match: GPX4_TOBAC (Probable phospholipid hydroperoxide glutathione peroxidase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 36.5798 bits (83), Expect = 4.077e-7
Identity = 19/26 (73.08%), Postives = 21/26 (80.77%), Query Frame = 1 
Query:    1 AEKSSK--SIYDFTVKDIRGNDVSLS 72
            A +SSK  SIYDFTVKD +GNDV LS
Sbjct:    2 ASQSSKPQSIYDFTVKDAKGNDVDLS 27

HSP 2 Score: 36.5798 bits (83), Expect = 4.077e-7
Identity = 14/29 (48.28%), Postives = 21/29 (72.41%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELNEFIRE 170
            G+   +VNVASQCGLT +NY ++ E  ++
Sbjct:   31 GKVLIIVNVASQCGLTNSNYTDMTEIYKK 59
[Close]
BLAST of AM162076 vs. SwissProt
Match: GPX1_HELAN (Glutathione peroxidase 1 OS=Helianthus annuus GN=GPXHA-1 PE=2 SV=1)

HSP 1 Score: 37.7354 bits (86), Expect = 5.286e-7
Identity = 16/24 (66.67%), Postives = 20/24 (83.33%), Query Frame = 1 
Query:    1 AEKSSKSIYDFTVKDIRGNDVSLS 72
            A +S K++YDFTVKD +GNDV LS
Sbjct:    2 ATQSKKTLYDFTVKDAKGNDVDLS 25

HSP 2 Score: 35.039 bits (79), Expect = 5.286e-7
Identity = 14/25 (56.00%), Postives = 19/25 (76.00%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELNE 158
            G+   +VNVAS+CGLT  +Y ELN+
Sbjct:   29 GKVVLIVNVASKCGLTNNSYDELNQ 53
[Close]
BLAST of AM162076 vs. TAIR peptide
Match: AT2G31570.1 (| Symbols: ATGPX2, GPX2 | glutathione peroxidase 2 | chr2:13438211-13439775 REVERSE LENGTH=169)

HSP 1 Score: 44.2838 bits (103), Expect = 4.797e-11
Identity = 23/34 (67.65%), Postives = 27/34 (79.41%), Query Frame = 1 
Query:    1 AEKSSKSIYDFTVKDIRGNDVSLSESQWEGSTAL 102
            A++S KSIYDFTVKDI GNDVSL   Q++G T L
Sbjct:    2 ADESPKSIYDFTVKDIGGNDVSL--DQYKGKTLL 33

HSP 2 Score: 38.5058 bits (88), Expect = 4.797e-11
Identity = 17/24 (70.83%), Postives = 19/24 (79.17%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELN 155
            G+   VVNVAS+CGLT  NYKELN
Sbjct:   29 GKTLLVVNVASKCGLTDANYKELN 52
[Close]
BLAST of AM162076 vs. TAIR peptide
Match: AT2G43350.2 (| Symbols: GPX3 | glutathione peroxidase 3 | chr2:18009195-18010533 REVERSE LENGTH=206)

HSP 1 Score: 36.965 bits (84), Expect = 8.907e-8
Identity = 15/24 (62.50%), Postives = 19/24 (79.17%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELN 155
            G+   +VNVAS+CGLT  NYKE+N
Sbjct:   68 GKVLLIVNVASKCGLTHGNYKEMN 91

HSP 2 Score: 34.6538 bits (78), Expect = 8.907e-8
Identity = 16/24 (66.67%), Postives = 20/24 (83.33%), Query Frame = 1 
Query:    4 EKSSKSIYDFTVKDIRGNDVSLSE 75
            E+SS SIY+ +VKDI G DVSLS+
Sbjct:   42 EQSSTSIYNISVKDIEGKDVSLSK 65
[Close]
BLAST of AM162076 vs. TAIR peptide
Match: AT2G43350.1 (| Symbols: ATGPX3, GPX3 | glutathione peroxidase 3 | chr2:18009195-18010533 REVERSE LENGTH=206)

HSP 1 Score: 36.965 bits (84), Expect = 8.907e-8
Identity = 15/24 (62.50%), Postives = 19/24 (79.17%), Query Frame = 3 
Query:   84 GRFYCVVNVASQCGLTQTNYKELN 155
            G+   +VNVAS+CGLT  NYKE+N
Sbjct:   68 GKVLLIVNVASKCGLTHGNYKEMN 91

HSP 2 Score: 34.6538 bits (78), Expect = 8.907e-8
Identity = 16/24 (66.67%), Postives = 20/24 (83.33%), Query Frame = 1 
Query:    4 EKSSKSIYDFTVKDIRGNDVSLSE 75
            E+SS SIY+ +VKDI G DVSLS+
Sbjct:   42 EQSSTSIYNISVKDIEGKDVSLSK 65
The following BLAST results are available for this feature:
BLAST of AM162076 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
Q8L5Q6_CICAR1.400e-1192.00Glutathione peroxidase OS=Cicer arietinum GN=phgpx... [more]
C6T3W7_SOYBN2.465e-1087.50Glutathione peroxidase OS=Glycine max PE=2 SV=1[more]
C6T020_SOYBN5.443e-1079.17Glutathione peroxidase OS=Glycine max PE=2 SV=1[more]
A5AU08_VITVI5.668e-984.00Glutathione peroxidase OS=Vitis vinifera GN=VITISV... [more]
D0PWB5_THEHA7.350e-970.59Glutathione peroxidase OS=Thellungiella halophila ... [more]
B9HH74_POPTR7.355e-984.00Glutathione peroxidase OS=Populus trichocarpa GN=P... [more]
Q8W259_MOMCH1.238e-870.59Glutathione peroxidase OS=Momordica charantia GN=P... [more]
D7RV80_BRANA2.703e-867.65Glutathione peroxidase 2 OS=Brassica napus GN=GPX2... [more]
D7RV79_BRANA2.703e-867.65Glutathione peroxidase 2 OS=Brassica napus GN=GPX2... [more]
A9NQB1_PICSI3.463e-875.00Glutathione peroxidase OS=Picea sitchensis PE=2 SV... [more]
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BLAST of AM162076 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 5
Match NameE-valueIdentityDescription
GPX2_ARATH6.154e-1067.65Probable glutathione peroxidase 2 OS=Arabidopsis t... [more]
GPX4_GOSHI2.435e-751.72Probable phospholipid hydroperoxide glutathione pe... [more]
GPX4_NICSY2.435e-751.72Probable phospholipid hydroperoxide glutathione pe... [more]
GPX4_TOBAC4.077e-773.08Probable phospholipid hydroperoxide glutathione pe... [more]
GPX1_HELAN5.286e-766.67Glutathione peroxidase 1 OS=Helianthus annuus GN=G... [more]
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BLAST of AM162076 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3
Match NameE-valueIdentityDescription
AT2G31570.14.797e-1167.65| Symbols: ATGPX2, GPX2 | glutathione peroxidase 2... [more]
AT2G43350.28.907e-862.50| Symbols: GPX3 | glutathione peroxidase 3 | chr2:... [more]
AT2G43350.18.907e-862.50| Symbols: ATGPX3, GPX3 | glutathione peroxidase 3... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000889Glutathione peroxidasePANTHERPTHR11592GLUTATHIONE PEROXIDASEcoord: 9..32
score: 1
IPR000889Glutathione peroxidasePFAMPF00255GSHPxcoord: 4..34
score: 1.5
IPR012335Thioredoxin foldGENE3D3.40.30.10no descriptioncoord: 4..32
score: 9.2

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>AM162076 ID=AM162076; Name=AM162076; organism=Pisum sativum; type=EST; length=240bp
GCCGAAAAATCCTCCAAATCCATATATGATTTCACTGTCAAGGATATCCG
TGGAAATGATGTGAGTTTGAGTGAATCACAGTGGGAAGGTTCTACTGCGT
TGTCAATGTTGCTTCACAATGTGGTTTGACACAAACGAATTACAAAGAGT
TGAATGAGTTCATACGAGAAGCACAAGCAATAATGGGTATTGCAAATCTT
GGACGTATATCCATGACACAACCAGATTATCGATTCACAG
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004602glutathione peroxidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006979response to oxidative stress
GO:0055114obsolete oxidation-reduction process
Vocabulary: INTERPRO
TermDefinition
IPR000889Glutathione_peroxidase
IPR012335Thioredoxin fold