CD859518

EST Overview


Name	CD859518
Unique Name	CD859518
Type	EST
Organism	Pisum sativum (pea)
Sequence length	357

Libraries


Library Name	Type
LIBEST_014000 F15	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of CD859518 vs. TrEMBL
Match: Q45NM6_MEDSA (Cystathionine-gamma-synthase (Fragment) OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 219.164 bits (557), Expect = 1.108e-55
Identity = 108/118 (91.53%), Postives = 115/118 (97.46%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LEEK+SELEGAEST+L+ASGMCASIV+LMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGI  SV+DPADVGAL+SALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHK+GALL ID
Sbjct:    3 LEEKISELEGAESTILMASGMCASIVLLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGIKTSVVDPADVGALQSALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKNGALLVID 120

[Close]

BLAST of CD859518 vs. TrEMBL
Match: Q0GC25_MEDSA (Cystathionine gamma-synthase OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 1.225e-54
Identity = 105/118 (88.98%), Postives = 112/118 (94.92%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LEEK+SELEGAEST+L+ASGMCAS+V+ MALVPAGGHLVTTTDCYRKTRIFIET LPKMGIT +VIDPADVGALESALEQ+ VSLFFTESPTNPFLRCVDIKLVSELCHK G LLCID
Sbjct:  210 LEEKISELEGAESTILMASGMCASVVLFMALVPAGGHLVTTTDCYRKTRIFIETFLPKMGITTTVIDPADVGALESALEQHNVSLFFTESPTNPFLRCVDIKLVSELCHKKGTLLCID 327

[Close]

BLAST of CD859518 vs. TrEMBL
Match: Q9XFG0_SOYBN (Cystathionine-gamma-synthase OS=Glycine max GN=cys PE=2 SV=1)

HSP 1 Score: 213.001 bits (541), Expect = 7.941e-54
Identity = 104/118 (88.14%), Postives = 111/118 (94.07%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LEEK+S LEGAESTV++ASGMCAS+V+ MALVPAGGHLVTTTDCYRKTRIFIET LPKMGIT +VIDPADVGALESALEQ+ VSLFFTESPTNPFLRCVDIKLVSELCHK G LLCID
Sbjct:  210 LEEKISALEGAESTVIMASGMCASVVLFMALVPAGGHLVTTTDCYRKTRIFIETFLPKMGITTTVIDPADVGALESALEQHNVSLFFTESPTNPFLRCVDIKLVSELCHKKGTLLCID 327

[Close]

BLAST of CD859518 vs. TrEMBL
Match: O23944_FRAVE (Cystathionine gamma synthase OS=Fragaria vesca GN=CGS PE=2 SV=2)

HSP 1 Score: 207.223 bits (526), Expect = 4.357e-52
Identity = 99/118 (83.90%), Postives = 112/118 (94.92%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LEEK+S LEGAEST+LLASGMCAS V+LMALVPAGGH+VTTTDCYRKTRIFIET+LPKMGITA++IDPADVGALE+AL +NKVSLFFTESPTNPFLRCVDI LVS+LCH  GA++CI+
Sbjct:  219 LEEKISALEGAESTLLLASGMCASTVLLMALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATIIDPADVGALETALNENKVSLFFTESPTNPFLRCVDINLVSDLCHSKGAVVCIN 336

[Close]

BLAST of CD859518 vs. TrEMBL
Match: Q9ZPL5_TOBAC (Cystathionine gamma-synthase OS=Nicotiana tabacum GN=metB PE=1 SV=1)

HSP 1 Score: 206.838 bits (525), Expect = 5.691e-52
Identity = 100/118 (84.75%), Postives = 112/118 (94.92%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LEEK+S LEGAEST+L+ASGMCAS VML+ALVPAGGH+VTTTDCYRKTRIFIET+LPKMGITA+VIDPADVGALE AL Q KV+LFFTESPTNPFLRCVDI+LVS+LCH+ GAL+CID
Sbjct:  119 LEEKISALEGAESTLLMASGMCASTVMLLALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATVIDPADVGALELALNQKKVNLFFTESPTNPFLRCVDIELVSKLCHEKGALVCID 236

[Close]

BLAST of CD859518 vs. TrEMBL
Match: B9IK07_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_835709 PE=3 SV=1)

HSP 1 Score: 206.838 bits (525), Expect = 5.691e-52
Identity = 101/118 (85.59%), Postives = 112/118 (94.92%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LE+K+SELEGAEST++LASGMCAS V+L+ALVPAGGHLVTTTDCYRKTRIF+ET LPKMGIT +VIDPADV ALESALE NKVSLFFTESPTNPFLRCVDI+LVSELCH+ GAL+CID
Sbjct:  203 LEDKISELEGAESTLILASGMCASTVLLLALVPAGGHLVTTTDCYRKTRIFMETFLPKMGITVTVIDPADVKALESALEINKVSLFFTESPTNPFLRCVDIELVSELCHRKGALVCID 320

[Close]

BLAST of CD859518 vs. TrEMBL
Match: O81334_MESCR (Cystathionine gamma-synthase OS=Mesembryanthemum crystallinum PE=2 SV=1)

HSP 1 Score: 206.453 bits (524), Expect = 7.432e-52
Identity = 99/118 (83.90%), Postives = 112/118 (94.92%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LE+K+S LEGAEST+LLASGMCASIV+++ALVPAGGH+VTTTDCYRKTRIFIET LPKMGITA+VIDPADV  L+SAL++N V+LFFTESPTNPFLRCVDIKLVSELCHK GAL+CID
Sbjct:  222 LEDKISALEGAESTILLASGMCASIVLMLALVPAGGHIVTTTDCYRKTRIFIETFLPKMGITATVIDPADVEGLKSALDENNVTLFFTESPTNPFLRCVDIKLVSELCHKKGALVCID 339

[Close]

BLAST of CD859518 vs. TrEMBL
Match: B9SVH8_RICCO (Cystathionine gamma-synthase, putative (Fragment) OS=Ricinus communis GN=RCOM_0537490 PE=3 SV=1)

HSP 1 Score: 206.068 bits (523), Expect = 9.707e-52
Identity = 99/117 (84.62%), Postives = 111/117 (94.87%), Query Frame = -3

Query:    2 EEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 352
            EEK+S LEGAEST+++ASGMCAS VML+ALVPAGGH+VTTTDCYRKTRIFIETVLPKMGITA+VIDPADV  LE+ALE+N VSLFFTESPTNPFLRCVDIKLVSELCH+ GA++CID
Sbjct:  100 EEKISALEGAESTLIMASGMCASTVMLLALVPAGGHIVTTTDCYRKTRIFIETVLPKMGITATVIDPADVEGLEAALEKNNVSLFFTESPTNPFLRCVDIKLVSELCHRKGAIVCID 216

[Close]

BLAST of CD859518 vs. TrEMBL
Match: Q9MT29_SOLTU (Cystathionine gamma-synthase isoform 2 OS=Solanum tuberosum GN=CgS2 PE=2 SV=1)

HSP 1 Score: 202.216 bits (513), Expect = 1.402e-50
Identity = 95/117 (81.20%), Postives = 111/117 (94.87%), Query Frame = -3

Query:    2 EEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 352
            EEK+S LEGA++T+L+ASGMCAS V+L+ALVPAGGH+VTTTDCYRKTRIFIET+LPKMGITA+VIDPAD+GALES L Q KV+LFFTESPTNPFLRCVDI+LVS+LCH+ GAL+CID
Sbjct:  215 EEKISALEGADATLLMASGMCASTVLLLALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATVIDPADIGALESVLNQKKVTLFFTESPTNPFLRCVDIELVSKLCHEKGALVCID 331

[Close]

BLAST of CD859518 vs. TrEMBL
Match: Q6R8F6_SOLLC (Cystathionine gamma synthase OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 199.134 bits (505), Expect = 1.187e-49
Identity = 95/118 (80.51%), Postives = 109/118 (92.37%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LEEK+S LEGAEST+++ASGMCAS VM +ALVPAGGH+VTTTDCYRKTR+FIET+LPKMGITA+VIDPAD+GALE AL Q KV LFFTESPTNPFLRCVDI+LVS+LC + GAL+CID
Sbjct:  214 LEEKISALEGAESTLIVASGMCASTVMFLALVPAGGHIVTTTDCYRKTRVFIETILPKMGITATVIDPADMGALELALNQKKVDLFFTESPTNPFLRCVDIELVSKLCREKGALVCID 331

[Close]

BLAST of CD859518 vs. SwissProt
Match: METB_ARATH (Cystathionine gamma-synthase, chloroplastic OS=Arabidopsis thaliana GN=CGS1 PE=2 SV=3)

HSP 1 Score: 191.43 bits (485), Expect = 1.206e-48
Identity = 89/118 (75.42%), Postives = 107/118 (90.68%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LE+K+S LEGAEST+++ASGMCAS VML+ALVPAGGH+VTTTDCYRKTRIF+E  LPK+GIT +VIDPAD+  LE+A+ + KVSLFFTESPTNPFLRCVDI+LVS++CHK G L+CID
Sbjct:  237 LEDKISALEGAESTLVMASGMCASTVMLLALVPAGGHIVTTTDCYRKTRIFMENFLPKLGITVTVIDPADIAGLEAAVNEFKVSLFFTESPTNPFLRCVDIELVSKICHKRGTLVCID 354

[Close]

BLAST of CD859518 vs. SwissProt
Match: METZ_PSEAE (O-succinylhomoserine sulfhydrylase OS=Pseudomonas aeruginosa GN=metZ PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 7.893e-16
Identity = 43/117 (36.75%), Postives = 66/117 (56.41%), Query Frame = -3

Query:    2 EEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 352
            EE+++ LEGAE  V  ASGM A + ++M+L  +G H++ +   +  T    +    + GI       +D+ A E+A + N   LFF ESP+NP    VDI  ++E+ H  GALL +D
Sbjct:   79 EERIAALEGAEQAVATASGMSAILALVMSLCSSGDHVLVSRSVFGSTISLFDKYFKRFGIQVDYPPLSDLAAWEAACKPN-TKLFFVESPSNPLAELVDIAALAEIAHAKGALLAVD 194

[Close]

BLAST of CD859518 vs. SwissProt
Match: MCCB_BACSU (Cystathionine gamma-lyase OS=Bacillus subtilis GN=mccB PE=1 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.788e-13
Identity = 41/118 (34.75%), Postives = 69/118 (58.47%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LE  ++ELE  E+    +SGM A+I  +M L  +G H+V T D Y  T   +  VL ++GI ++ +D +    +E A+  N  +++  E+PTNP L+  D+ L++++  K G LL +D
Sbjct:   55 LEALVTELESGEAGYAFSSGM-AAITAVMMLFNSGDHVVLTDDVYGGTYRVMTKVLNRLGIESTFVDTSSREEVEKAIRPNTKAIYI-ETPTNPLLKITDLTLMADIAKKAGVLLIVD 170

[Close]

BLAST of CD859518 vs. SwissProt
Match: METC_LACLM (Cystathionine beta-lyase OS=Lactococcus lactis subsp. cremoris (strain MG1363) GN=metC PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.393e-12
Identity = 39/118 (33.05%), Postives = 70/118 (59.32%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LEE +++LEG       +SG+ A I  +++L  AG H++   D Y  T   ++ VL K GI   ++D +++  L++A ++   +++F E+P+NP L+ +DIK +S +   H AL  +D
Sbjct:   56 LEELIADLEGGVQGFAFSSGL-AGIHAVLSLFSAGDHIILADDVYGGTFRLMDKVLTKTGIIYDLVDLSNLDDLKAAFKEETKAIYF-ETPSNPLLKVLDIKEISAIAKAHDALTLVD 171

[Close]

BLAST of CD859518 vs. SwissProt
Match: METC_LACLA (Cystathionine beta-lyase OS=Lactococcus lactis subsp. lactis GN=metC PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.393e-12
Identity = 39/118 (33.05%), Postives = 70/118 (59.32%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LEE +++LEG       +SG+ A I  +++L  AG H++   D Y  T   ++ VL K GI   ++D +++  L++A ++   +++F E+P+NP L+ +DIK +S +   H AL  +D
Sbjct:   56 LEELIADLEGGVQGFAFSSGL-AGIHAVLSLFSAGDHIILADDVYGGTFRLMDKVLTKTGIIYDLVDLSNLDDLKAAFKEETKAIYF-ETPSNPLLKVLDIKEISAIAKAHDALTLVD 171

[Close]

BLAST of CD859518 vs. SwissProt
Match: METC_LACLC (Cystathionine beta-lyase OS=Lactococcus lactis subsp. cremoris GN=metC PE=1 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.820e-12
Identity = 39/118 (33.05%), Postives = 69/118 (58.47%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LEE +++LEG       +SG+ A I  +++L  AG H++   D Y  T   ++ VL K GI   ++D +++  L++A +    +++F E+P+NP L+ +DIK +S +   H AL  +D
Sbjct:   56 LEELIADLEGGVQGFAFSSGL-AGIHAVLSLFSAGDHIILADDVYGGTFRLVDKVLTKTGIIYDLVDLSNLEDLKAAFKAETKAVYF-ETPSNPLLKVLDIKEISSIAKAHNALTLVD 171

[Close]

BLAST of CD859518 vs. SwissProt
Match: METC_RHIL3 (Putative cystathionine beta-lyase OS=Rhizobium leguminosarum bv. viciae (strain 3841) GN=metC PE=3 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 3.104e-12
Identity = 36/116 (31.03%), Postives = 61/116 (52.59%), Query Frame = -3

Query:    2 EKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 349
            E +  LEG+  T+L+ SG+ A  +  +  V AG H +     Y  TR F +T+L ++G+      P ++GA    L ++   L  TE+P +      DI  +S + H+HGA++ +D
Sbjct:   71 EAIDALEGSAGTILVPSGLAAVTIPFLGFVAAGDHALVVDSVYGPTRHFCDTMLKRLGVEVEYYHP-EIGAGIETLFRSNTKLVHTEAPGSNTFEMQDIPAISAVAHRHGAVVMMD 185

[Close]

BLAST of CD859518 vs. SwissProt
Match: CGL_PIG (Cystathionine gamma-lyase OS=Sus scrofa GN=CTH PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 9.031e-12
Identity = 35/117 (29.91%), Postives = 69/117 (58.97%), Query Frame = -3

Query:    5 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCI 355
            LE+ ++ L+GA+ ++  ASG+ A++  +  L+ AG  +++  D Y  T  +   V  + G+  S +D +    LE+A+   +  L + E+PTNP L+ +DI+  +++ HKHG ++ +
Sbjct:   71 LEKAVAVLDGAKYSLAFASGLAATVT-ITHLLKAGXQIISMDDVYGGTNRYFRQVAAEFGLKISFVDCSKSKLLEAAITP-ETKLVWIETPTNPILKMIDIEACAQIVHKHGDIILV 185

[Close]

BLAST of CD859518 vs. SwissProt
Match: METB_HELPY (Cystathionine gamma-synthase OS=Helicobacter pylori GN=metB PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.179e-11
Identity = 37/118 (31.36%), Postives = 66/118 (55.93%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LEE +++LEG       ASG+ A I  + +L+ +G H++   D Y  T      VL K G++ ++ID +D+  ++ A++ N  +L+  E+P+NP L+  D+   + +   HG L  +D
Sbjct:   55 LEELIADLEGGVKGFAFASGL-AGIHAVFSLLQSGDHVLLGDDVYGGTFRLFNQVLVKNGLSCTIIDTSDISQIKKAIKPNTKALYL-ETPSNPLLKITDLAQCASVAKDHGLLTIVD 170

[Close]

BLAST of CD859518 vs. SwissProt
Match: MEGL_PSEPU (Methionine gamma-lyase OS=Pseudomonas putida GN=mdeA PE=1 SV=2)

HSP 1 Score: 67.3958 bits (163), Expect = 2.627e-11
Identity = 40/118 (33.90%), Postives = 64/118 (54.24%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LE +++ LEG E+ + LASGM A    L  L+  G  ++     Y  T  F+   + + G+    +D AD+ ALE+A+      ++F ESP NP +   DI  V+++  KHGA + +D
Sbjct:   70 LEARMASLEGGEAGLALASGMGAITSTLWTLLRPGDEVLLGNTLYGCTFAFLHHGIGEFGVKLRHVDMADLQALEAAMTPATRVIYF-ESPANPNMHMADIAGVAKIARKHGATVVVD 186

[Close]

BLAST of CD859518 vs. TAIR peptide
Match: AT3G01120.1 (| Symbols: MTO1, CGS, ATCYS1, CGS1 | Pyridoxal phosphate (PLP)-dependent transferases superfamily protein | chr3:39234-41865 REVERSE LENGTH=563)

HSP 1 Score: 191.43 bits (485), Expect = 9.290e-50
Identity = 89/118 (75.42%), Postives = 107/118 (90.68%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LE+K+S LEGAEST+++ASGMCAS VML+ALVPAGGH+VTTTDCYRKTRIF+E  LPK+GIT +VIDPAD+  LE+A+ + KVSLFFTESPTNPFLRCVDI+LVS++CHK G L+CID
Sbjct:  237 LEDKISALEGAESTLVMASGMCASTVMLLALVPAGGHIVTTTDCYRKTRIFMENFLPKLGITVTVIDPADIAGLEAAVNEFKVSLFFTESPTNPFLRCVDIELVSKICHKRGTLVCID 354

[Close]

BLAST of CD859518 vs. TAIR peptide
Match: AT1G33320.1 (| Symbols: | Pyridoxal phosphate (PLP)-dependent transferases superfamily protein | chr1:12081009-12083462 FORWARD LENGTH=412)

HSP 1 Score: 162.54 bits (410), Expect = 4.621e-41
Identity = 76/118 (64.41%), Postives = 97/118 (82.20%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LEEK+S LEGAEST+++ASGM AS VML+ALVP  GH+V T DCY++TRIF+E  L K+GIT + ID  D+  L++ +  ++VSLFFTESPTNPFLRCVDIKLVS++CH+ G L+CID
Sbjct:   87 LEEKISVLEGAESTLVMASGMYASNVMLLALVPTNGHIVATKDCYKETRIFMENFLTKLGITVTFIDSDDIAGLQTLVNNHEVSLFFTESPTNPFLRCVDIKLVSKICHRRGTLVCID 204

[Close]

BLAST of CD859518 vs. TAIR peptide
Match: AT1G64660.1 (| Symbols: ATMGL, MGL | methionine gamma-lyase | chr1:24028977-24030537 FORWARD LENGTH=441)

HSP 1 Score: 69.707 bits (169), Expect = 4.077e-13
Identity = 36/119 (30.25%), Postives = 63/119 (52.94%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPK-MGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            L  +++ LEG ++    +SGM A   +++ L  +GGH+V  +  Y  T   +   LP+   IT S +D  D GA+ +A+ + +  + + ES  NP L   DI  +S + H+ G  + +D
Sbjct:  106 LSRQMAALEGTQAAYCTSSGMSAISSVMLQLCSSGGHVVAASTLYGGTHALLSHFLPRTCNITTSFVDITDHGAVANAIVEGRTQVLYFESVANPTLTVADIPELSRMAHEKGVTVVVD 224

[Close]

BLAST of CD859518 vs. TAIR peptide
Match: AT3G57050.2 (| Symbols: CBL | cystathionine beta-lyase | chr3:21111939-21114521 REVERSE LENGTH=449)

HSP 1 Score: 57.7658 bits (138), Expect = 1.603e-9
Identity = 34/118 (28.81%), Postives = 62/118 (52.54%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LE  L++L+ A+      SGM A++  +  L+  G  +V   D Y  +   +  V+P+ G+    ++   +  + +A+   +  L + ESPTNP  +  DI+ +SE+ H  GAL+ +D
Sbjct:  123 LESLLAKLDKADRAFCFTSGM-AALSAVTHLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLDEVAAAIGP-QTKLVWLESPTNPRQQISDIRKISEMAHAQGALVLVD 238

[Close]

BLAST of CD859518 vs. TAIR peptide
Match: AT3G57050.3 (| Symbols: CBL | cystathionine beta-lyase | chr3:21112392-21114521 REVERSE LENGTH=378)

HSP 1 Score: 57.7658 bits (138), Expect = 1.603e-9
Identity = 34/118 (28.81%), Postives = 62/118 (52.54%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LE  L++L+ A+      SGM A++  +  L+  G  +V   D Y  +   +  V+P+ G+    ++   +  + +A+   +  L + ESPTNP  +  DI+ +SE+ H  GAL+ +D
Sbjct:  138 LESLLAKLDKADRAFCFTSGM-AALSAVTHLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLDEVAAAIGP-QTKLVWLESPTNPRQQISDIRKISEMAHAQGALVLVD 253

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BLAST of CD859518 vs. TAIR peptide
Match: AT3G57050.1 (| Symbols: CBL | cystathionine beta-lyase | chr3:21111939-21114521 REVERSE LENGTH=464)

HSP 1 Score: 57.7658 bits (138), Expect = 1.603e-9
Identity = 34/118 (28.81%), Postives = 62/118 (52.54%), Query Frame = -3

Query:    2 LEEKLSELEGAESTVLLASGMCASIVMLMALVPAGGHLVTTTDCYRKTRIFIETVLPKMGITASVIDPADVGALESALEQNKVSLFFTESPTNPFLRCVDIKLVSELCHKHGALLCID 355
            LE  L++L+ A+      SGM A++  +  L+  G  +V   D Y  +   +  V+P+ G+    ++   +  + +A+   +  L + ESPTNP  +  DI+ +SE+ H  GAL+ +D
Sbjct:  138 LESLLAKLDKADRAFCFTSGM-AALSAVTHLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLDEVAAAIGP-QTKLVWLESPTNPRQQISDIRKISEMAHAQGALVLVD 253

The following BLAST results are available for this feature:

BLAST of CD859518 vs. TrEMBL
BLAST of CD859518 vs. SwissProt
BLAST of CD859518 vs. TAIR peptide

BLAST of CD859518 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q45NM6_MEDSA	1.108e-55	91.53	Cystathionine-gamma-synthase (Fragment) OS=Medicag...	[more]
Q0GC25_MEDSA	1.225e-54	88.98	Cystathionine gamma-synthase OS=Medicago sativa PE...	[more]
Q9XFG0_SOYBN	7.941e-54	88.14	Cystathionine-gamma-synthase OS=Glycine max GN=cys...	[more]
O23944_FRAVE	4.357e-52	83.90	Cystathionine gamma synthase OS=Fragaria vesca GN=...	[more]
Q9ZPL5_TOBAC	5.691e-52	84.75	Cystathionine gamma-synthase OS=Nicotiana tabacum ...	[more]
B9IK07_POPTR	5.691e-52	85.59	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
O81334_MESCR	7.432e-52	83.90	Cystathionine gamma-synthase OS=Mesembryanthemum c...	[more]
B9SVH8_RICCO	9.707e-52	84.62	Cystathionine gamma-synthase, putative (Fragment) ...	[more]
Q9MT29_SOLTU	1.402e-50	81.20	Cystathionine gamma-synthase isoform 2 OS=Solanum ...	[more]
Q6R8F6_SOLLC	1.187e-49	80.51	Cystathionine gamma synthase OS=Solanum lycopersic...	[more]

BLAST of CD859518 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
METB_ARATH	1.206e-48	75.42	Cystathionine gamma-synthase, chloroplastic OS=Ara...	[more]
METZ_PSEAE	7.893e-16	36.75	O-succinylhomoserine sulfhydrylase OS=Pseudomonas ...	[more]
MCCB_BACSU	4.788e-13	34.75	Cystathionine gamma-lyase OS=Bacillus subtilis GN=...	[more]
METC_LACLM	1.393e-12	33.05	Cystathionine beta-lyase OS=Lactococcus lactis sub...	[more]
METC_LACLA	1.393e-12	33.05	Cystathionine beta-lyase OS=Lactococcus lactis sub...	[more]
METC_LACLC	1.820e-12	33.05	Cystathionine beta-lyase OS=Lactococcus lactis sub...	[more]
METC_RHIL3	3.104e-12	31.03	Putative cystathionine beta-lyase OS=Rhizobium leg...	[more]
CGL_PIG	9.031e-12	29.91	Cystathionine gamma-lyase OS=Sus scrofa GN=CTH PE=...	[more]
METB_HELPY	1.179e-11	31.36	Cystathionine gamma-synthase OS=Helicobacter pylor...	[more]
MEGL_PSEPU	2.627e-11	33.90	Methionine gamma-lyase OS=Pseudomonas putida GN=md...	[more]

BLAST of CD859518 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT3G01120.1	9.290e-50	75.42	\| Symbols: MTO1, CGS, ATCYS1, CGS1 \| Pyridoxal pho...	[more]
AT1G33320.1	4.621e-41	64.41	\| Symbols: \| Pyridoxal phosphate (PLP)-dependent ...	[more]
AT1G64660.1	4.077e-13	30.25	\| Symbols: ATMGL, MGL \| methionine gamma-lyase \| c...	[more]
AT3G57050.2	1.603e-9	28.81	\| Symbols: CBL \| cystathionine beta-lyase \| chr3:2...	[more]
AT3G57050.3	1.603e-9	28.81	\| Symbols: CBL \| cystathionine beta-lyase \| chr3:2...	[more]
AT3G57050.1	1.603e-9	28.81	\| Symbols: CBL \| cystathionine beta-lyase \| chr3:2...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PANTHER	PTHR11808	TRANS-SULFURATION ENZYME FAMILY MEMBER	coord: 28..118 score: 1.8
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PFAM	PF01053	Cys_Met_Meta_PP	coord: 1..118 score: 3.5
IPR015421	Pyridoxal phosphate-dependent transferase, major region, subdomain 1	GENE3D	3.40.640.10	no description	coord: 1..118 score: 2.7
IPR015424	Pyridoxal phosphate-dependent transferase, major domain	SUPERFAMILY	53383	PLP-dependent transferases	coord: 1..118 score: 1.3
None	No IPR available	PANTHER	PTHR11808:SF14	CYSTATHIONINE GAMMA-SYNTHASE	coord: 28..118 score: 1.8

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD859518 ID=CD859518; Name=CD859518; organism=Pisum sativum; type=EST; length=357bp
CATCAATGCAGAGCAAAGCCCCATGCTTGTGGCAAAGCTCTGAAACCAGC
TTAATGTCAACACATCTCAGGAATGGATTGGTAGGAGACTCGGTGAAGAA
TAGAGACACCTTGTTCTGCTCCAATGCAGATTCCAAGGCTCCAACATCAG
CAGGATCAATCACAGAGGCCGTGATCCCCATCTTCGGAAGCACAGTCTCT
ATAAATATCCTAGTCTTCCTGTAACAATCAGTTGTAGTCACAAGATGTCC
ACCGGCTGGAACCAGCGCCATGAGCATAACTATGCTAGCACACATTCCAG
ACGCCAATAAGACAGTTGACTCAGCCCCTTCCAGTTCACTTAATTTCTCC
TCTAAAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006520	amino acid metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0030170	pyridoxal phosphate binding
GO:0003824	catalytic activity

Vocabulary: **INTERPRO**
Term	Definition
IPR000277	Cys/Met-Metab_PyrdxlP-dep_enz
IPR015421	PyrdxlP-dep_Trfase_major
IPR015424	PyrdxlP-dep_Trfase

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