AJ419712

EST Overview


Name	AJ419712
Unique Name	AJ419712
Type	EST
Organism	Pisum sativum (pea)
Sequence length	353

Libraries


Library Name	Type
LIBEST_010632 Pisum sativum arbuscular mycorrhiza	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of AJ419712 vs. TrEMBL
Match: Q9LEN3_CICAR (Putative uncharacterized protein (Fragment) OS=Cicer arietinum PE=2 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 1.239e-27
Identity = 60/76 (78.95%), Postives = 66/76 (86.84%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            NF IIGGTGLADTLEKVSFKSQL +  NGG+IR VHV+YFTKGDY L+EEELKA K KVEGL+K  +GYLLANPDY
Sbjct:   16 NFSIIGGTGLADTLEKVSFKSQLFDDENGGTIRKVHVEYFTKGDYQLTEEELKAGKDKVEGLIKICEGYLLANPDY 91

[Close]

BLAST of AJ419712 vs. TrEMBL
Match: C6T376_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 9.818e-25
Identity = 57/76 (75.00%), Postives = 63/76 (82.89%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            NF I  GT LADTLEKVSF+SQLVE PNGGSIR V V +FTKGD  LSEEEL A+KAK++GLVK V+GYLL NPDY
Sbjct:   82 NFSITEGTALADTLEKVSFESQLVEAPNGGSIRKVSVQFFTKGDATLSEEELTANKAKIQGLVKLVEGYLLVNPDY 157

[Close]

BLAST of AJ419712 vs. TrEMBL
Match: Q94IM3_MEDSA (Class 10 PR protein OS=Medicago sativa GN=pr10.2 PE=3 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.767e-19
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            N+ I+GG  L DT+EK+SF+++L  GPNGGSI  + V YFTKGD   SEEELK+ KAK +GL K ++GY LANPDY
Sbjct:   82 NYSIVGGDSLPDTVEKISFEAKLSAGPNGGSIAKLSVKYFTKGDATPSEEELKSGKAKGDGLFKALEGYCLANPDY 157

[Close]

BLAST of AJ419712 vs. TrEMBL
Match: C6T3L5_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.767e-19
Identity = 45/76 (59.21%), Postives = 60/76 (78.95%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            N+ ++GG GL DT+EK+SF+++LVEG +GGSI  + V Y TKGD N SEEELK+ KAK + L K ++GY+LANPDY
Sbjct:   82 NYSVVGGVGLPDTVEKISFEAKLVEGASGGSIAKITVKYQTKGDVNPSEEELKSGKAKGDALFKALEGYVLANPDY 157

[Close]

BLAST of AJ419712 vs. TrEMBL
Match: C6T206_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 3.614e-19
Identity = 45/76 (59.21%), Postives = 59/76 (77.63%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            N+ ++GG GL DT+EK+SF+++LV  PNGGSI  + V Y TKGD N SEEELK+ KAK + L K ++GY+LANPDY
Sbjct:   82 NYSVVGGVGLPDTVEKISFEAKLVADPNGGSIAKITVKYQTKGDANPSEEELKSGKAKGDALFKALEGYVLANPDY 157

[Close]

BLAST of AJ419712 vs. TrEMBL
Match: Q7FNV7_MEDSA (Class 10 PR protein OS=Medicago sativa PE=3 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 8.050e-19
Identity = 45/76 (59.21%), Postives = 59/76 (77.63%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            N+ I+GG GL DT+EK+SF+S+L  GP+GGSI  + V YFTKGD   SEEE+K  KA+ +GL K ++GY+LANPDY
Sbjct:   82 NYSIVGGGGLPDTVEKISFESKLSAGPDGGSIAKLTVKYFTKGDAAPSEEEIKGGKARGDGLFKALEGYVLANPDY 157

[Close]

BLAST of AJ419712 vs. TrEMBL
Match: P93333_MEDTR (PR10-1 protein OS=Medicago truncatula GN=PR10-1 PE=2 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 8.050e-19
Identity = 45/76 (59.21%), Postives = 59/76 (77.63%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            N+ I+GG GL DT+EK+SF+S+L  GP+GGSI  + V YFTKGD   SEEE+K  KA+ +GL K ++GY+LANPDY
Sbjct:   82 NYSIVGGGGLPDTVEKISFESKLSAGPDGGSIAKLTVKYFTKGDAAPSEEEIKGGKARGDGLFKALEGYVLANPDY 157

[Close]

BLAST of AJ419712 vs. TrEMBL
Match: Q9SAT3_PEA (Root hair protein RH2 (Fragment) OS=Pisum sativum GN=rh2 PE=2 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 1.373e-18
Identity = 44/76 (57.89%), Postives = 58/76 (76.32%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            N+ I+GG GL DT+EK+SF+++L  GPNGGSI  + V Y+TKGD   SEEE+K  KAK EG+ K ++GY +ANPDY
Sbjct:   79 NYSIVGGVGLPDTVEKISFEAKLSAGPNGGSIAKLSVKYYTKGDAIPSEEEIKNGKAKGEGIFKALEGYCVANPDY 154

[Close]

BLAST of AJ419712 vs. TrEMBL
Match: O24253_PEA (Pathogenesis-related protein PR10 (Fragment) OS=Pisum sativum subsp. elatius GN=Ypr10.Pi.ela.3 PE=3 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 1.373e-18
Identity = 44/76 (57.89%), Postives = 58/76 (76.32%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            N+ I+GG GL DT+EK+SF+++L  GPNGGSI  + V Y+TKGD   SEEE+K  KAK EG+ K ++GY +ANPDY
Sbjct:   35 NYSIVGGVGLPDTVEKISFEAKLSAGPNGGSIAKLSVKYYTKGDAIPSEEEIKNGKAKGEGIFKALEGYCVANPDY 110

[Close]

BLAST of AJ419712 vs. TrEMBL
Match: Q40320_MEDSA (Srg1 OS=Medicago sativa GN=SRG1 PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.059e-18
Identity = 44/76 (57.89%), Postives = 58/76 (76.32%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            N+ I+GG GL DT+EK+SF+S+L  GP+GGS   + V YFTKGD   SEEE+K  KA+ +GL K ++GY+LANPDY
Sbjct:   82 NYSIVGGGGLPDTVEKISFESKLSAGPDGGSTAKLTVKYFTKGDAAPSEEEIKGGKARGDGLFKALEGYVLANPDY 157

[Close]

BLAST of AJ419712 vs. SwissProt
Match: DRR4_PEA (Disease resistance response protein DRRG49-C OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 6.924e-20
Identity = 44/76 (57.89%), Postives = 58/76 (76.32%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            N+ I+GG GL DT+EK+SF+++L  GPNGGSI  + V Y+TKGD   SEEE+K  KAK EG+ K ++GY +ANPDY
Sbjct:   82 NYSIVGGVGLPDTVEKISFEAKLSAGPNGGSIAKLSVKYYTKGDAIPSEEEIKNGKAKGEGIFKALEGYCVANPDY 157

[Close]

BLAST of AJ419712 vs. SwissProt
Match: DRR3_PEA (Disease resistance response protein Pi49 OS=Pisum sativum GN=DRR49A PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.181e-19
Identity = 44/76 (57.89%), Postives = 57/76 (75.00%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            N+ I+GG G  DT+EK+SF+++L  GPNGGSI  + V YFTKGD   SEE+LK  KAK +GL K ++GY LA+PDY
Sbjct:   82 NYSIVGGVGFPDTVEKISFEAKLSAGPNGGSIAKLSVKYFTKGDAAPSEEQLKTDKAKGDGLFKALEGYCLAHPDY 157

[Close]

BLAST of AJ419712 vs. SwissProt
Match: PR1_MEDSA (Class-10 pathogenesis-related protein 1 OS=Medicago sativa GN=MSPR10-1 PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.543e-19
Identity = 44/76 (57.89%), Postives = 58/76 (76.32%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            N+ I+GG GL DT+EK+SF+S+L  GP+GGS   + V YFTKGD   SEEE+K  KA+ +GL K ++GY+LANPDY
Sbjct:   82 NYSIVGGGGLPDTVEKISFESKLSAGPDGGSTAKLTVKYFTKGDAAPSEEEIKGGKARGDGLFKALEGYVLANPDY 157

[Close]

BLAST of AJ419712 vs. SwissProt
Match: DRR1_PEA (Disease resistance response protein Pi176 OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 5.486e-17
Identity = 41/77 (53.25%), Postives = 57/77 (74.03%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNL-SEEELKASKAKVEGLVKFVDGYLLANPDY 230
            N+ I+GG G  DT+EK+SF+++L  GPNGGSI  + V Y+TKGD    +EE+LK+ KAK +GL K ++ Y LA+PDY
Sbjct:   82 NYSIVGGVGFPDTVEKISFEAKLSAGPNGGSIAKLSVKYYTKGDAAAPTEEQLKSDKAKGDGLFKALERYCLAHPDY 158

[Close]

BLAST of AJ419712 vs. SwissProt
Match: ABR18_PEA (ABA-responsive protein ABR18 OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.596e-16
Identity = 37/76 (48.68%), Postives = 56/76 (73.68%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            N+ I+GG G++D +EK+SF+++L EGPNGGS+  + V Y TKGD    E+E++  KAK + L K ++ Y+LANP+Y
Sbjct:   82 NYSIVGGVGISDIVEKISFEAKLFEGPNGGSVGKMIVKYHTKGDAKPIEKEVEEGKAKGDALFKAIEAYVLANPNY 157

[Close]

BLAST of AJ419712 vs. SwissProt
Match: SAM22_SOYBN (Stress-induced protein SAM22 OS=Glycine max PE=1 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 2.085e-16
Identity = 38/76 (50.00%), Postives = 53/76 (69.74%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            ++ ++GG  L DT EK++F S+LV GPNGGS   + V Y TKGD   +++ELK  KAK + L K ++ YLLA+PDY
Sbjct:   82 SYSVVGGAALPDTAEKITFDSKLVAGPNGGSAGKLTVKYETKGDAEPNQDELKTGKAKADALFKAIEAYLLAHPDY 157

[Close]

BLAST of AJ419712 vs. SwissProt
Match: L18B_LUPLU (Protein LlR18B OS=Lupinus luteus GN=LLR18B PE=1 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.035e-15
Identity = 39/76 (51.32%), Postives = 58/76 (76.32%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            N+ I+GGTGL ++LEK++F+S+L+ GP+GGSI  + V + TKGD  LS+   + +KA+  GL K V+GY+LANP+Y
Sbjct:   82 NYSIVGGTGLDESLEKITFESKLLSGPDGGSIGKIKVKFHTKGDV-LSDAVREEAKARGTGLFKAVEGYVLANPNY 156

[Close]

BLAST of AJ419712 vs. SwissProt
Match: PR2_PHAVU (Pathogenesis-related protein 2 OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 1.765e-15
Identity = 37/74 (50.00%), Postives = 52/74 (70.27%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANP 224
            ++ I+GG  L DT EK+S  S+L +GPNGGS+  + + Y +KGD   +E+ELKA KAK + L K ++ YLLANP
Sbjct:   82 SYSIVGGAALPDTAEKISIDSKLSDGPNGGSVVKLSIKYHSKGDAPPNEDELKAGKAKSDALFKVIEAYLLANP 155

[Close]

BLAST of AJ419712 vs. SwissProt
Match: PR1_PHAVU (Pathogenesis-related protein 1 OS=Phaseolus vulgaris PE=1 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 3.010e-15
Identity = 37/74 (50.00%), Postives = 53/74 (71.62%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANP 224
            ++ I+GG  L +T EK++F S+L +GPNGGS+  + + Y +KGD   +E+ELKA KAK + L K V+ YLLANP
Sbjct:   83 SYSIVGGVALPETAEKITFDSKLSDGPNGGSLIKLSITYHSKGDAPPNEDELKAGKAKSDSLFKAVEAYLLANP 156

[Close]

BLAST of AJ419712 vs. SwissProt
Match: L18A_LUPLU (Protein LlR18A OS=Lupinus luteus GN=LLR18A PE=1 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.951e-14
Identity = 38/76 (50.00%), Postives = 56/76 (73.68%), Query Frame = 3

Query:    3 NFGIIGGTGLADTLEKVSFKSQLVEGPNGGSIRNVHVDYFTKGDYNLSEEELKASKAKVEGLVKFVDGYLLANPDY 230
            N+ IIGG GL ++LEK+S++S+++ GP+GGSI  ++V + TKGD  LSE     +K K  GL K ++GY+LA+PDY
Sbjct:   82 NYSIIGGEGLDESLEKISYESKILPGPDGGSIGKINVKFHTKGDV-LSETVRDQAKFKGLGLFKAIEGYVLAHPDY 156

The following BLAST results are available for this feature:

BLAST of AJ419712 vs. TrEMBL
BLAST of AJ419712 vs. SwissProt

BLAST of AJ419712 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9LEN3_CICAR	1.239e-27	78.95	Putative uncharacterized protein (Fragment) OS=Cic...	[more]
C6T376_SOYBN	9.818e-25	75.00	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q94IM3_MEDSA	2.767e-19	60.53	Class 10 PR protein OS=Medicago sativa GN=pr10.2 P...	[more]
C6T3L5_SOYBN	2.767e-19	59.21	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6T206_SOYBN	3.614e-19	59.21	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q7FNV7_MEDSA	8.050e-19	59.21	Class 10 PR protein OS=Medicago sativa PE=3 SV=1	[more]
P93333_MEDTR	8.050e-19	59.21	PR10-1 protein OS=Medicago truncatula GN=PR10-1 PE...	[more]
Q9SAT3_PEA	1.373e-18	57.89	Root hair protein RH2 (Fragment) OS=Pisum sativum ...	[more]
O24253_PEA	1.373e-18	57.89	Pathogenesis-related protein PR10 (Fragment) OS=Pi...	[more]
Q40320_MEDSA	3.059e-18	57.89	Srg1 OS=Medicago sativa GN=SRG1 PE=2 SV=1	[more]

BLAST of AJ419712 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
DRR4_PEA	6.924e-20	57.89	Disease resistance response protein DRRG49-C OS=Pi...	[more]
DRR3_PEA	1.181e-19	57.89	Disease resistance response protein Pi49 OS=Pisum ...	[more]
PR1_MEDSA	1.543e-19	57.89	Class-10 pathogenesis-related protein 1 OS=Medicag...	[more]
DRR1_PEA	5.486e-17	53.25	Disease resistance response protein Pi176 OS=Pisum...	[more]
ABR18_PEA	1.596e-16	48.68	ABA-responsive protein ABR18 OS=Pisum sativum PE=2...	[more]
SAM22_SOYBN	2.085e-16	50.00	Stress-induced protein SAM22 OS=Glycine max PE=1 S...	[more]
L18B_LUPLU	1.035e-15	51.32	Protein LlR18B OS=Lupinus luteus GN=LLR18B PE=1 SV...	[more]
PR2_PHAVU	1.765e-15	50.00	Pathogenesis-related protein 2 OS=Phaseolus vulgar...	[more]
PR1_PHAVU	3.010e-15	50.00	Pathogenesis-related protein 1 OS=Phaseolus vulgar...	[more]
L18A_LUPLU	1.951e-14	50.00	Protein LlR18A OS=Lupinus luteus GN=LLR18A PE=1 SV...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000916	Bet v I allergen	PRINTS	PR00634	BETALLERGEN	coord: 3..16 score: 2.5e-11coord: 28..44 score: 2.5e-11coord: 62..72 score: 2.5
IPR000916	Bet v I allergen	PFAM	PF00407	Bet_v_1	coord: 2..73 score: 3.2
None	No IPR available	GENE3D	3.30.530.20	no description	coord: 2..76 score: 1.4
None	No IPR available	SUPERFAMILY	55961	Bet v1-like	coord: 4..76 score: 6.8

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AJ419712 ID=AJ419712; Name=AJ419712; organism=Pisum sativum; type=EST; length=353bp
ACAACTTTGGCATTATTGGTGGCACTGGCTTAGCTGACACATTGGAGAAA
GTCTCATTCAAGAGCCAATTGGTGGAAGGTCCAAATGGAGGATCCATTAG
GAATGTGCATGTTGATTATTTTACCAAAGGTGATTACAATCTTAGTGAGG
AGGAGCTGAAAGCTAGTAAAGCTAAGGTTGAAGGACTTGTTAAGTTTGTT
GATGGTTACCTTTTGGCAAATCCTGATTATTGAATGTCTTTWATTAGGGT
TAATAAAAWCTTAGCTATGGTGAAAGTTAATAATAGCCTAAGTTGAAGAT
TTGGTCGTAAGATGAWGCATAAGTTTGAGTGATGAGATTGTTTTTGTATT
TGC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006952	defense response
GO:0009607	response to biotic stimulus

Vocabulary: **INTERPRO**
Term	Definition
IPR000916	Bet_v_I/MLP

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