BU964317

EST Overview


Name	BU964317
Unique Name	BU964317
Type	EST
Organism	Pisum sativum (pea)
Sequence length	509

Libraries


Library Name	Type
LIBEST_012000 Pea suppression subtractive hybridisation library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v2	Pisum sativum unigene v2	EST
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Pisum sativum unigene v2	2011-03-01
Homology Analysis: Pisum sativum unigene v2 vs Trembl	2011-04-27
Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins	2011-04-21
Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides	2011-04-21
InterProScan analysis for Pisum sativum unigene v2	2011-04-27
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of BU964317 vs. TrEMBL
Match: Q2PER5_TRIPR (Putative early nodulin-like 2 predicted GPI-anchored protein (Fragment) OS=Trifolium pratense PE=2 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 6.238e-7
Identity = 24/39 (61.54%), Postives = 27/39 (69.23%), Query Frame = 3

Query:    6 KGSDSVLEVNKEAYDKCNXXNXXNKXXDGXTEXTLDRSG 122
            KGSDS+LEV KE Y+KCN  N   K  DG TE T D+SG
Sbjct:   47 KGSDSLLEVKKEDYEKCNKTNPIKKFEDGETEFTFDKSG 85

HSP 2 Score: 24.6386 bits (52), Expect = 6.238e-7
Identity = 11/17 (64.71%), Postives = 12/17 (70.59%), Query Frame = 2

Query:  164 KKGXKXTLVXISPXGHT 214
            +KG K TLV ISP  HT
Sbjct:   98 EKGQKLTLVVISPRKHT 114

[Close]

BLAST of BU964317 vs. TrEMBL
Match: Q2PER5_TRIPR (Putative early nodulin-like 2 predicted GPI-anchored protein (Fragment) OS=Trifolium pratense PE=2 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 7.582e-7
Identity = 24/39 (61.54%), Postives = 27/39 (69.23%), Query Frame = 3

Query:    6 KGSDSVLEVNKEAYDKCNXXNXXNKXXDGXTEXTLDRSG 122
            KGSDS+LEV KE Y+KCN  N   K  DG TE T D+SG
Sbjct:   47 KGSDSLLEVKKEDYEKCNKTNPIKKFEDGETEFTFDKSG 85

HSP 2 Score: 24.6386 bits (52), Expect = 7.582e-7
Identity = 11/17 (64.71%), Postives = 12/17 (70.59%), Query Frame = 2

Query:  164 KKGXKXTLVXISPXGHT 214
            +KG K TLV ISP  HT
Sbjct:   98 EKGQKLTLVVISPRKHT 114

[Close]

BLAST of BU964317 vs. Lotus protein
Match: chr3.CM0282.1000.r2.m (- phase: 0 )

HSP 1 Score: 52.7582 bits (125), Expect = 1.550e-8
Identity = 25/39 (64.10%), Postives = 26/39 (66.67%), Query Frame = 3

Query:    6 KGSDSVLEVNKEAYDKCNXXNXXNKXXDGXTEXTLDRSG 122
            KGSDSVLEV KE +D CN  N   K  DG TE T DRSG
Sbjct:   62 KGSDSVLEVKKEDFDNCNKANPIKKFEDGDTEFTFDRSG 100

HSP 2 Score: 21.9422 bits (45), Expect = 1.550e-8
Identity = 10/18 (55.56%), Postives = 11/18 (61.11%), Query Frame = 2

Query:  164 KKGXKXTLVXISPXGHTP 217
            +KG K  LV ISP G  P
Sbjct:  113 EKGQKMILVVISPRGTQP 130

[Close]

BLAST of BU964317 vs. Soybean peptides
Match: Glyma06g36590.1|PACid:16264382 ()

HSP 1 Score: 54.299 bits (129), Expect = 6.351e-8
Identity = 26/40 (65.00%), Postives = 27/40 (67.50%), Query Frame = 3

Query:    3 NKGSDSVLEVNKEAYDKCNXXNXXNKXXDGXTEXTLDRSG 122
            NKGSDSVLEV KE YDKCN  N   K  +G TE   DRSG
Sbjct:   61 NKGSDSVLEVKKEDYDKCNKTNPIKKFENGDTEFKFDRSG 100

The following BLAST results are available for this feature:

BLAST of BU964317 vs. TrEMBL
BLAST of BU964317 vs. TrEMBL
BLAST of BU964317 vs. Lotus protein
BLAST of BU964317 vs. Soybean peptides

BLAST of BU964317 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 1


Match Name	E-value	Identity	Description
Q2PER5_TRIPR	6.238e-7	61.54	Putative early nodulin-like 2 predicted GPI-anchor...	[more]

BLAST of BU964317 vs. TrEMBL
Analysis Date: 2011-04-27 (Homology Analysis: Pisum sativum unigene v2 vs Trembl)
Total hits: 1


Match Name	E-value	Identity	Description
Q2PER5_TRIPR	7.582e-7	61.54	Putative early nodulin-like 2 predicted GPI-anchor...	[more]

BLAST of BU964317 vs. Lotus protein
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins)
Total hits: 1


Match Name	E-value	Identity	Description
chr3.CM0282.1000.r2.m	1.550e-8	64.10	- phase: 0	[more]

BLAST of BU964317 vs. Soybean peptides
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides)
Total hits: 1


Match Name	E-value	Identity	Description
Glyma06g36590.1\|PACid:16264382	6.351e-8	65.00		[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v2
Date Performed: 2011-04-27


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 11..24 score: NAcoord: 32..43 score: NAcoord: 65..77 score
None	No IPR available	SEG	seg	seg	coord: 3..8 score: NAcoord: 26..43 score: NAcoord: 60..122 score: NAcoord: 134..143 score
None	No IPR available	SEG	seg	seg	coord: 19..25 score: NAcoord: 43..52 score: NAcoord: 91..103 score: NAcoord: 116..134 score
None	No IPR available	SEG	seg	seg	coord: 10..19 score: NAcoord: 43..69 score: NAcoord: 74..93 score: NAcoord: 110..122 score: NAcoord: 145..150 score
None	No IPR available	SEG	seg	seg	coord: 42..51 score: NAcoord: 68..77 score
None	No IPR available	SEG	seg	seg	coord: 10..22 score: NAcoord: 46..55 score: NAcoord: 58..87 score
IPR003245	Plastocyanin-like	PFAM	PF02298	Cu_bind_like	coord: 2..41 score: 5.3
IPR008972	Cupredoxin	GENE3D	2.60.40.420	no description	coord: 2..41 score: 7

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 11..24 score: NAcoord: 32..43 score: NAcoord: 65..77 score
None	No IPR available	SEG	seg	seg	coord: 3..8 score: NAcoord: 26..43 score: NAcoord: 60..122 score: NAcoord: 134..143 score
None	No IPR available	SEG	seg	seg	coord: 19..25 score: NAcoord: 43..52 score: NAcoord: 91..103 score: NAcoord: 116..134 score
None	No IPR available	SEG	seg	seg	coord: 10..19 score: NAcoord: 43..69 score: NAcoord: 74..93 score: NAcoord: 110..122 score: NAcoord: 145..150 score
None	No IPR available	SEG	seg	seg	coord: 42..51 score: NAcoord: 68..77 score
None	No IPR available	SEG	seg	seg	coord: 10..22 score: NAcoord: 46..55 score: NAcoord: 58..87 score
IPR003245	Plastocyanin-like	PFAM	PF02298	Cu_bind_like	coord: 2..41 score: 5.3
IPR008972	Cupredoxin	GENE3D	2.60.40.420	no description	coord: 2..41 score: 7

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v2
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>BU964317 ID=BU964317; Name=BU964317; organism=Pisum sativum; type=EST; length=509bp
ACAACAAGGGTTCAGACTCTGTGTTGGAAGTGAACAAAGAAGCTTATGAT
AAATGCAACRAARCRAACCSRRTSAACAAGTTKGRAGATGGKRASACRGA
GTTWACTTTGGATCGATCTGGAACCATTSKAGWKKMATKAGKGGAGRGRK
GRKRARARTTGKGAAAAAGGGTCRGAAATKAACCCTTGTCGKTATATCCC
CASGAGGCCACACACCRAAATGCGGCTCCCTTCGCCGTCTCCCTCTCTTT
CGGMTTCTNCCCCMTGCAAATCCACCCTCTCTATCACCATCCTTCAGWAK
GCCTCCCGCCGGTGAACCTACGGCTCCACCTCCTACGRCAGGTGGACCAC
CTGYYCCATCACCGATCTCTCCTTCCACGGAAGCTTCACCGCCATCTCCA
TCTCCAACTATTCCAGCAGATGGTGSTGCGGCATCGGGTGCACAGTCTCT
GGCACCTGGAGGAGCAGCGGTTCCACCGGCGTCATCTAATTCAATAGCGC
CTTTGAGGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005507	copper ion binding
GO:0009055	electron transfer activity

Vocabulary: **INTERPRO**
Term	Definition
IPR003245	Phytocyanin_dom
IPR008972	Cupredoxin

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