CD859451

EST Overview


Name	CD859451
Unique Name	CD859451
Type	EST
Organism	Pisum sativum (pea)
Sequence length	249

Libraries


Library Name	Type
LIBEST_014000 F15	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of CD859451 vs. TrEMBL
Match: C6SZN6_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 3.148e-26
Identity = 60/82 (73.17%), Postives = 66/82 (80.49%), Query Frame = -2

Query:    3 SDRKIGVALDFSKGSKIALKWTVDNLVRHGDTLYVILINHSKSSESRNHLWLTTGSPLIPLSEFREKNVTHQYEVVPDADVL 248
            SDR IGVALDFSKGSKIALKW +DNL+R+GD LY++ I  S  SE RN LW TTGSPLIPLSEFREK V H YEV  DA+VL
Sbjct:    3 SDRNIGVALDFSKGSKIALKWAIDNLLRNGDILYIVHIKPSGGSEFRNLLWSTTGSPLIPLSEFREKEVMHHYEVDTDAEVL 84

[Close]

BLAST of CD859451 vs. TrEMBL
Match: C6SVX3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 2.040e-25
Identity = 59/82 (71.95%), Postives = 65/82 (79.27%), Query Frame = -2

Query:    3 SDRKIGVALDFSKGSKIALKWTVDNLVRHGDTLYVILINHSKSSESRNHLWLTTGSPLIPLSEFREKNVTHQYEVVPDADVL 248
            SDRKIGVALDFSKGSKIALKW +DNL+ +GDTLY++    S  SES N LW TTGSPLIPLSEFREK V   YEV  DA+VL
Sbjct:    3 SDRKIGVALDFSKGSKIALKWAIDNLISNGDTLYIVHTKPSGGSESGNLLWSTTGSPLIPLSEFREKEVMRHYEVDTDAEVL 84

[Close]

BLAST of CD859451 vs. TrEMBL
Match: B0YQX0_GOSAR (Universal stress protein 1 OS=Gossypium arboreum GN=USP1 PE=2 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.013e-24
Identity = 56/81 (69.14%), Postives = 65/81 (80.25%), Query Frame = -2

Query:    3 DRKIGVALDFSKGSKIALKWTVDNLVRHGDTLYVILINHSKSSESRNHLWLTTGSPLIPLSEFREKNVTHQYEVVPDADVL 245
            DR+IGVA+DFSKGSK ALKWT+DNLV  GDTLY+I +  ++S ESR  LW TTGSPLIPLSEFREK V   YEV PD ++L
Sbjct:    4 DRQIGVAMDFSKGSKAALKWTIDNLVDKGDTLYLIHVKPNQSDESRKLLWSTTGSPLIPLSEFREKEVMKHYEVEPDPEIL 84

[Close]

BLAST of CD859451 vs. TrEMBL
Match: D7SK62_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_4.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00024846001 PE=4 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 5.750e-20
Identity = 48/81 (59.26%), Postives = 60/81 (74.07%), Query Frame = -2

Query:    3 DRKIGVALDFSKGSKIALKWTVDNLVRHGDTLYVILINHSKSSESRNHLWLTTGSPLIPLSEFREKNVTHQYEVVPDADVL 245
            DRKIGVA+DFS+GS IALKW +DNL+  GDTL+ I +  S+  ESRN LW  TGSPLIPL EFR+ +V  +YE+  D + L
Sbjct:    4 DRKIGVAVDFSQGSNIALKWAIDNLLDKGDTLFFIHVKPSQGDESRNLLWSATGSPLIPLEEFRDLDVAQKYEINLDPEFL 84

[Close]

BLAST of CD859451 vs. TrEMBL
Match: B9S3A2_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0731860 PE=4 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 5.750e-20
Identity = 47/81 (58.02%), Postives = 60/81 (74.07%), Query Frame = -2

Query:    3 DRKIGVALDFSKGSKIALKWTVDNLVRHGDTLYVILINHSKSSESRNHLWLTTGSPLIPLSEFREKNVTHQYEVVPDADVL 245
            DR IGVA+DFSKGSK+AL W + NL+ +GDTLY+I +   +  ESR  LW  TGSPLIPL EFRE+ V ++YE+  D +VL
Sbjct:    4 DRNIGVAMDFSKGSKLALNWAITNLIDNGDTLYIIHVKPQQGDESRLLLWSATGSPLIPLVEFREQEVANKYEIKLDPEVL 84

[Close]

BLAST of CD859451 vs. TrEMBL
Match: C6T3L1_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 7.509e-20
Identity = 48/81 (59.26%), Postives = 60/81 (74.07%), Query Frame = -2

Query:    3 DRKIGVALDFSKGSKIALKWTVDNLVRHGDTLYVILINHSKSSESRNHLWLTTGSPLIPLSEFREKNVTHQYEVVPDADVL 245
            DR +GVALDFSK SKIALKW ++NL   G TLY+I +N   SS+ RN LW+ +GSPL+PL+EFR+  VT  Y V  DA+VL
Sbjct:    4 DRNVGVALDFSKSSKIALKWAIENLADKGQTLYIIHVN-PNSSDDRNQLWVKSGSPLVPLTEFRDAEVTKHYGVQTDAEVL 83

[Close]

BLAST of CD859451 vs. TrEMBL
Match: C6SZP4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 2.853e-19
Identity = 49/81 (60.49%), Postives = 58/81 (71.60%), Query Frame = -2

Query:    3 DRKIGVALDFSKGSKIALKWTVDNLVRHGDTLYVILINHSKSSESRNHLWLTTGSPLIPLSEFREKNVTHQYEVVPDADVL 245
            DR IGVALDFSK SK ALKW ++NL   GDT+YVI IN +   ESRN LW  +GSPLIPL EFRE  +  +Y+V  D +VL
Sbjct:    4 DRTIGVALDFSKSSKNALKWALENLADKGDTIYVIHINSNSLDESRNKLWAGSGSPLIPLVEFREPEIMKKYDVQIDIEVL 84

[Close]

BLAST of CD859451 vs. TrEMBL
Match: C6SYI8_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.867e-19
Identity = 49/81 (60.49%), Postives = 57/81 (70.37%), Query Frame = -2

Query:    3 DRKIGVALDFSKGSKIALKWTVDNLVRHGDTLYVILINHSKSSESRNHLWLTTGSPLIPLSEFREKNVTHQYEVVPDADVL 245
            DR IGVALDFSK SK ALKW  +NL   GDT+YVI IN +   ESRN LW  +GSPLIPL EFRE  +  +Y+V  D +VL
Sbjct:    4 DRTIGVALDFSKSSKNALKWAFENLADKGDTIYVIHINPNSLDESRNKLWAKSGSPLIPLVEFREPEIMKKYDVEIDIEVL 84

[Close]

BLAST of CD859451 vs. TrEMBL
Match: B0YQX1_GOSAR (Universal stress protein 2 OS=Gossypium arboreum GN=USP2 PE=2 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 6.357e-19
Identity = 56/115 (48.70%), Postives = 65/115 (56.52%), Query Frame = -2

Query:    3 DRKIGVALDFSKGSKIALKWTVDNLVRHGDTLYVILINHSKSSESRNHLWLTTGS----------------------------------PLIPLSEFREKNVTHQYEVVPDADVL 245
            DR+IGVA+DFSKGSK ALKWT+DNLV  GDTLY+I +  ++S ESRN LW T+GS                                   LIPLSEFREK V   YEV PD +VL
Sbjct:    4 DRQIGVAMDFSKGSKAALKWTIDNLVDKGDTLYLIHVKPNQSDESRNLLWSTSGSRKSLPYSTSVFDQVLKLWVIGILTIFDLGDLLFVALIPLSEFREKEVMKHYEVEPDPEVL 118

[Close]

BLAST of CD859451 vs. TrEMBL
Match: B9T763_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0358800 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 8.302e-19
Identity = 46/81 (56.79%), Postives = 57/81 (70.37%), Query Frame = -2

Query:    3 DRKIGVALDFSKGSKIALKWTVDNLVRHGDTLYVILINHSKSSESRNHLWLTTGSPLIPLSEFREKNVTHQYEVVPDADVL 245
            DR  GVA+DFSK SK ALKW +DNL   GDTLY+I ++ +   ESRN LW  +GSPLIPL++FRE  V   Y+V  D +VL
Sbjct:    4 DRNFGVAMDFSKSSKSALKWAIDNLADRGDTLYLIHVSPNSLDESRNQLWAKSGSPLIPLAQFREPEVMRGYDVKIDIEVL 84

[Close]

BLAST of CD859451 vs. TAIR peptide
Match: AT3G53990.1 (| Symbols: | Adenine nucleotide alpha hydrolases-like superfamily protein | chr3:19989658-19991019 REVERSE LENGTH=160)

HSP 1 Score: 88.1965 bits (217), Expect = 1.145e-18
Identity = 41/77 (53.25%), Postives = 52/77 (67.53%), Query Frame = -2

Query:   15 DRKIGVALDFSKGSKIALKWTVDNLVRHGDTLYVILINHSKSSESRNHLWLTTGSPLIPLSEFREKNVTHQYEVVPD 245
            DR IG+A+DFS+ SK ALKW ++NL   GDT+Y+I        ESRN LW  +GSPLIPL+EFRE  +  +Y V  D
Sbjct:    4 DRNIGIAMDFSESSKNALKWAIENLADKGDTIYIIHTLPLSGDESRNSLWFKSGSPLIPLAEFREPEIMEKYGVKTD 80

[Close]

BLAST of CD859451 vs. TAIR peptide
Match: AT3G53990.2 (| Symbols: | Adenine nucleotide alpha hydrolases-like superfamily protein | chr3:19990558-19991019 REVERSE LENGTH=126)

HSP 1 Score: 88.1965 bits (217), Expect = 1.145e-18
Identity = 41/77 (53.25%), Postives = 52/77 (67.53%), Query Frame = -2

Query:   15 DRKIGVALDFSKGSKIALKWTVDNLVRHGDTLYVILINHSKSSESRNHLWLTTGSPLIPLSEFREKNVTHQYEVVPD 245
            DR IG+A+DFS+ SK ALKW ++NL   GDT+Y+I        ESRN LW  +GSPLIPL+EFRE  +  +Y V  D
Sbjct:    4 DRNIGIAMDFSESSKNALKWAIENLADKGDTIYIIHTLPLSGDESRNSLWFKSGSPLIPLAEFREPEIMEKYGVKTD 80

[Close]

BLAST of CD859451 vs. TAIR peptide
Match: AT3G17020.1 (| Symbols: | Adenine nucleotide alpha hydrolases-like superfamily protein | chr3:5802728-5804063 REVERSE LENGTH=163)

HSP 1 Score: 80.1073 bits (196), Expect = 3.119e-16
Identity = 41/81 (50.62%), Postives = 52/81 (64.20%), Query Frame = -2

Query:    3 RKIGVALDFSKGSKIALKWTVDNLVRHGDTLYVILINHSKS-SESRNHLWLTTGSPLIPLSEFREKNVTHQYEVVPDADVL 242
            R+IGVA+DFS  SK AL W +DN+VR GD L +I I H  +  E    LW T GSP IP+SEF +  V  +Y + PDA+ L
Sbjct:    7 RRIGVAVDFSDCSKKALSWAIDNVVRDGDHLILITIAHDMNYEEGEMQLWETVGSPFIPMSEFSDAAVMKKYALKPDAETL 87

[Close]

BLAST of CD859451 vs. TAIR peptide
Match: AT3G03270.2 (| Symbols: | Adenine nucleotide alpha hydrolases-like superfamily protein | chr3:762136-763075 REVERSE LENGTH=159)

HSP 1 Score: 77.0258 bits (188), Expect = 2.641e-15
Identity = 36/80 (45.00%), Postives = 53/80 (66.25%), Query Frame = -2

Query:    3 RKIGVALDFSKGSKIALKWTVDNLVRHGDTLYVILINHSKSSESRNHLWLTTGSPLIPLSEFREKNVTHQYEVVPDADVL 242
            R +GV +D+S  SK+AL+W  +NL+  GDT+ +I +    +  +R  L+  TGSPLIPL EFRE N++ QY +  D +VL
Sbjct:    5 RTVGVGMDYSPTSKLALRWAAENLLEDGDTVILIHVQPQNADHTRKILFEETGSPLIPLEEFREVNLSKQYGLAYDPEVL 84

[Close]

BLAST of CD859451 vs. TAIR peptide
Match: AT3G03270.1 (| Symbols: | Adenine nucleotide alpha hydrolases-like superfamily protein | chr3:761383-763075 REVERSE LENGTH=201)

HSP 1 Score: 77.0258 bits (188), Expect = 2.641e-15
Identity = 36/80 (45.00%), Postives = 53/80 (66.25%), Query Frame = -2

Query:    3 RKIGVALDFSKGSKIALKWTVDNLVRHGDTLYVILINHSKSSESRNHLWLTTGSPLIPLSEFREKNVTHQYEVVPDADVL 242
            R +GV +D+S  SK+AL+W  +NL+  GDT+ +I +    +  +R  L+  TGSPLIPL EFRE N++ QY +  D +VL
Sbjct:    5 RTVGVGMDYSPTSKLALRWAAENLLEDGDTVILIHVQPQNADHTRKILFEETGSPLIPLEEFREVNLSKQYGLAYDPEVL 84

The following BLAST results are available for this feature:

BLAST of CD859451 vs. TrEMBL
BLAST of CD859451 vs. TAIR peptide

BLAST of CD859451 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6SZN6_SOYBN	3.148e-26	73.17	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6SVX3_SOYBN	2.040e-25	71.95	Putative uncharacterized protein OS=Glycine max PE...	[more]
B0YQX0_GOSAR	1.013e-24	69.14	Universal stress protein 1 OS=Gossypium arboreum G...	[more]
D7SK62_VITVI	5.750e-20	59.26	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9S3A2_RICCO	5.750e-20	58.02	Putative uncharacterized protein OS=Ricinus commun...	[more]
C6T3L1_SOYBN	7.509e-20	59.26	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6SZP4_SOYBN	2.853e-19	60.49	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6SYI8_SOYBN	4.867e-19	60.49	Putative uncharacterized protein OS=Glycine max PE...	[more]
B0YQX1_GOSAR	6.357e-19	48.70	Universal stress protein 2 OS=Gossypium arboreum G...	[more]
B9T763_RICCO	8.302e-19	56.79	Putative uncharacterized protein OS=Ricinus commun...	[more]

BLAST of CD859451 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT3G53990.1	1.145e-18	53.25	\| Symbols: \| Adenine nucleotide alpha hydrolases-...	[more]
AT3G53990.2	1.145e-18	53.25	\| Symbols: \| Adenine nucleotide alpha hydrolases-...	[more]
AT3G17020.1	3.119e-16	50.62	\| Symbols: \| Adenine nucleotide alpha hydrolases-...	[more]
AT3G03270.2	2.641e-15	45.00	\| Symbols: \| Adenine nucleotide alpha hydrolases-...	[more]
AT3G03270.1	2.641e-15	45.00	\| Symbols: \| Adenine nucleotide alpha hydrolases-...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006016	UspA	PFAM	PF00582	Usp	coord: 3..65 score: 1.3
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	no description	coord: 3..51 score: 3.6

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD859451 ID=CD859451; Name=CD859451; organism=Pisum sativum; type=EST; length=249bp
TCCAAAACGTCAGCATCAGGCACCACCTCATACTGGTGAGTCACGTTCTT
CTCACGAAACTCGGACAACGGGATAAGAGGAGAACCGGTGGTTAACCATA
GATGGTTACGAGATTCAGAGGATTTGGAATGATTGATGAGAATAACATAG
AGAGTGTCACCGTGGCGGACAAGATTATCAACAGTCCATTTGAGAGCGAT
CTTGCTTCCCTTGGAAAAATCTAAAGCTACCCCGATTTTCCTATCGCTC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006950	response to stress

Vocabulary: **INTERPRO**
Term	Definition
IPR006016	UspA
IPR014729	Rossmann-like_a/b/a_fold

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