GH720227

EST Overview


Name	GH720227
Unique Name	GH720227
Type	EST
Organism	Pisum sativum (pea)
Sequence length	426

Libraries


Library Name	Type
LIBEST_014001 G15	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of GH720227 vs. TrEMBL
Match: Q43671_VICFA (Storage protein OS=Vicia faba var. minor PE=4 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 3.823e-32
Identity = 69/76 (90.79%), Postives = 74/76 (97.37%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQSQSH 230
            QNFVV EQAGEEEGLEYVVFKTNDRAAVSHV QVFRATP++VLANAFGLRQRQVTELKLSGNRGPL+HP+SQSQS+
Sbjct:  233 QNFVVEEQAGEEEGLEYVVFKTNDRAAVSHVHQVFRATPADVLANAFGLRQRQVTELKLSGNRGPLIHPQSQSQSN 308

[Close]

BLAST of GH720227 vs. TrEMBL
Match: Q41703_VICSA (Legumin B OS=Vicia sativa PE=2 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 2.739e-30
Identity = 68/75 (90.67%), Postives = 71/75 (94.67%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQSQS 227
            QNFVVAEQAGE EGLEYVVFKTNDRAAVSHVQQVFRATP++VLANAFGLRQRQVTELKLSGN GPLVH + QSQS
Sbjct:  410 QNFVVAEQAGENEGLEYVVFKTNDRAAVSHVQQVFRATPADVLANAFGLRQRQVTELKLSGNDGPLVHRQPQSQS 484

[Close]

BLAST of GH720227 vs. TrEMBL
Match: O24294_PEA (Legumin (Minor small) OS=Pisum sativum GN=legS PE=2 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 1.191e-25
Identity = 58/72 (80.56%), Postives = 63/72 (87.50%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQ 218
            QNFVVA+QAG EEG EYVVFKTNDRAAVSHV QVFRATP EVLANAFGLR  QV ++K +GNRGPLV P+SQ
Sbjct:  495 QNFVVAQQAGNEEGFEYVVFKTNDRAAVSHVNQVFRATPGEVLANAFGLRHSQVAQIKSNGNRGPLVQPQSQ 566

[Close]

BLAST of GH720227 vs. TrEMBL
Match: Q43673_VICFA (Legumin; legumin-related high molecular weight polypeptide OS=Vicia faba var. minor GN=LelB3 PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 3.465e-25
Identity = 57/75 (76.00%), Postives = 64/75 (85.33%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQSQS 227
            QNFVVA+QAG EE  EYVVFKTNDRAAVSHV QVFRATP+EVLAN FGLR  +V ++K +GNRGPLV P+SQ QS
Sbjct:  489 QNFVVAQQAGNEEAFEYVVFKTNDRAAVSHVNQVFRATPAEVLANVFGLRHSEVAQIKSNGNRGPLVQPQSQPQS 563

[Close]

BLAST of GH720227 vs. TrEMBL
Match: B5U8K5_LOTJA (Legumin storage protein 3 (Fragment) OS=Lotus japonicus GN=llp3 PE=2 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 4.684e-22
Identity = 55/76 (72.37%), Postives = 68/76 (89.47%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHP-RSQSQS 227
            QNFVVA+QA ++EG EYVVFKTN RAAVSHV+QVFRATP++VLANAFG+RQR V++LK SGN GPL++P  +QS+S
Sbjct:  415 QNFVVAQQA-QDEGFEYVVFKTNARAAVSHVKQVFRATPAQVLANAFGIRQRDVSDLKFSGNWGPLINPDNTQSRS 489

[Close]

BLAST of GH720227 vs. TrEMBL
Match: B5U8K2_LOTJA (Legumin storage protein 3 OS=Lotus japonicus GN=llp3 PE=3 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 4.684e-22
Identity = 55/76 (72.37%), Postives = 68/76 (89.47%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHP-RSQSQS 227
            QNFVVA+QA ++EG EYVVFKTN RAAVSHV+QVFRATP++VLANAFG+RQR V++LK SGN GPL++P  +QS+S
Sbjct:  531 QNFVVAQQA-QDEGFEYVVFKTNARAAVSHVKQVFRATPAQVLANAFGIRQRDVSDLKFSGNWGPLINPDNTQSRS 605

[Close]

BLAST of GH720227 vs. TrEMBL
Match: B5U8K1_LOTJA (Legumin storage protein 2 OS=Lotus japonicus GN=llp2 PE=3 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 4.684e-22
Identity = 55/76 (72.37%), Postives = 68/76 (89.47%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHP-RSQSQS 227
            QNFVVA+QA ++EG EYVVFKTN RAAVSHV+QVFRATP+EVL+NAFG+RQR +++LK SGN GPLV+P  +QS+S
Sbjct:  500 QNFVVAQQA-QDEGFEYVVFKTNARAAVSHVKQVFRATPAEVLSNAFGIRQRDISDLKFSGNWGPLVNPDNTQSRS 574

[Close]

BLAST of GH720227 vs. TrEMBL
Match: Q9SB11_SOYBN (Glycinin OS=Glycine max GN=A5A4B3 PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 4.103e-18
Identity = 48/75 (64.00%), Postives = 59/75 (78.67%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQSQS 227
            QNFVVAEQAGE+ G EY+VFKT+  A  S+++ VFRA PSEVLA+++ LRQ QV+ELK  GN GPLV+P SQ  S
Sbjct:  484 QNFVVAEQAGEQ-GFEYIVFKTHHNAVTSYLKDVFRAIPSEVLAHSYNLRQSQVSELKYEGNWGPLVNPESQQGS 557

[Close]

BLAST of GH720227 vs. TrEMBL
Match: Q43452_SOYBN (Glycinin OS=Glycine max GN=Gy4 PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 4.103e-18
Identity = 48/75 (64.00%), Postives = 59/75 (78.67%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQSQS 227
            QNFVVAEQAGE+ G EY+VFKT+  A  S+++ VFRA PSEVLA+++ LRQ QV+ELK  GN GPLV+P SQ  S
Sbjct:  483 QNFVVAEQAGEQ-GFEYIVFKTHHNAVTSYLKDVFRAIPSEVLAHSYNLRQSQVSELKYEGNWGPLVNPESQQGS 556

[Close]

BLAST of GH720227 vs. TrEMBL
Match: Q39921_GLYSO (A5A4B3 subunit OS=Glycine soja GN=glycinin Gy4 PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 4.103e-18
Identity = 48/75 (64.00%), Postives = 59/75 (78.67%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQSQS 227
            QNFVVAEQAGE+ G EY+VFKT+  A  S+++ VFRA PSEVLA+++ LRQ QV+ELK  GN GPLV+P SQ  S
Sbjct:  484 QNFVVAEQAGEQ-GFEYIVFKTHHNAVTSYLKDVFRAIPSEVLAHSYNLRQSQVSELKYEGNWGPLVNPESQQGS 557

[Close]

BLAST of GH720227 vs. SwissProt
Match: LEGJ_PEA (Legumin J OS=Pisum sativum GN=LEGJ PE=1 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 1.069e-36
Identity = 76/76 (100.00%), Postives = 76/76 (100.00%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQSQSH 230
            QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQSQSH
Sbjct:  428 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQSQSH 503

[Close]

BLAST of GH720227 vs. SwissProt
Match: LEGK_PEA (Legumin K (Fragment) OS=Pisum sativum GN=LEGK PE=3 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 3.440e-35
Identity = 73/76 (96.05%), Postives = 75/76 (98.68%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQSQSH 230
            QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQV RATP+EVLANAFGLRQRQVTELKLSGNRGPLVHP+SQSQSH
Sbjct:  275 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVLRATPAEVLANAFGLRQRQVTELKLSGNRGPLVHPQSQSQSH 350

[Close]

BLAST of GH720227 vs. SwissProt
Match: LEGB6_VICFA (Legumin type B (Fragment) OS=Vicia faba GN=LEB6 PE=3 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 2.230e-34
Identity = 71/76 (93.42%), Postives = 76/76 (100.00%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQSQSH 230
            QNFVVAEQAGEEEGLEY+VFKTNDRAAVSHVQQVFRATP++VLANAFGLRQRQVTELKLSGNRGPLVHP+SQSQS+
Sbjct:  254 QNFVVAEQAGEEEGLEYLVFKTNDRAAVSHVQQVFRATPADVLANAFGLRQRQVTELKLSGNRGPLVHPQSQSQSN 329

[Close]

BLAST of GH720227 vs. SwissProt
Match: LEGB4_VICFA (Legumin type B OS=Vicia faba GN=LEB4 PE=3 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 2.230e-34
Identity = 71/76 (93.42%), Postives = 76/76 (100.00%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQSQSH 230
            QNFVVAEQAGEEEGLEY+VFKTNDRAAVSHVQQVFRATP++VLANAFGLRQRQVTELKLSGNRGPLVHP+SQSQS+
Sbjct:  409 QNFVVAEQAGEEEGLEYLVFKTNDRAAVSHVQQVFRATPADVLANAFGLRQRQVTELKLSGNRGPLVHPQSQSQSN 484

[Close]

BLAST of GH720227 vs. SwissProt
Match: LEGB2_VICFA (Legumin type B (Fragment) OS=Vicia faba GN=LEB2 PE=3 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 2.230e-34
Identity = 71/76 (93.42%), Postives = 76/76 (100.00%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQSQSH 230
            QNFVVAEQAGEEEGLEY+VFKTNDRAAVSHVQQVFRATP++VLANAFGLRQRQVTELKLSGNRGPLVHP+SQSQS+
Sbjct:  260 QNFVVAEQAGEEEGLEYLVFKTNDRAAVSHVQQVFRATPADVLANAFGLRQRQVTELKLSGNRGPLVHPQSQSQSN 335

[Close]

BLAST of GH720227 vs. SwissProt
Match: LEGB7_VICFA (Legumin type B (Fragment) OS=Vicia faba GN=LEB7 PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 2.912e-34
Identity = 71/76 (93.42%), Postives = 75/76 (98.68%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQSQSH 230
            QNFVVAEQAGEEEGLEY+VFKTNDRAAVSHVQQVFRATP++VLANAFGLRQRQVTELKLSGNRGPLVHP SQSQS+
Sbjct:  260 QNFVVAEQAGEEEGLEYLVFKTNDRAAVSHVQQVFRATPADVLANAFGLRQRQVTELKLSGNRGPLVHPHSQSQSN 335

[Close]

BLAST of GH720227 vs. SwissProt
Match: LEGB_PEA (Legumin B (Fragment) OS=Pisum sativum GN=LEGB PE=2 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 5.881e-27
Identity = 58/72 (80.56%), Postives = 63/72 (87.50%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQ 218
            QNFVVA+QAG EEG EYVVFKTNDRAAVSHV QVFRATP EVLANAFGLR  QV ++K +GNRGPLV P+SQ
Sbjct:  267 QNFVVAQQAGNEEGFEYVVFKTNDRAAVSHVNQVFRATPGEVLANAFGLRHSQVAQIKSNGNRGPLVQPQSQ 338

[Close]

BLAST of GH720227 vs. SwissProt
Match: GLYG4_SOYBN (Glycinin G4 OS=Glycine max GN=GY4 PE=1 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 2.026e-19
Identity = 48/75 (64.00%), Postives = 59/75 (78.67%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHPRSQSQS 227
            QNFVVAEQAGE+ G EY+VFKT+  A  S+++ VFRA PSEVLA+++ LRQ QV+ELK  GN GPLV+P SQ  S
Sbjct:  483 QNFVVAEQAGEQ-GFEYIVFKTHHNAVTSYLKDVFRAIPSEVLAHSYNLRQSQVSELKYEGNWGPLVNPESQQGS 556

[Close]

BLAST of GH720227 vs. SwissProt
Match: GLYG5_SOYBN (Glycinin OS=Glycine max PE=1 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 3.028e-15
Identity = 41/69 (59.42%), Postives = 51/69 (73.91%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHVQQVFRATPSEVLANAFGLRQRQVTELKLSGNRGPLVHP 209
            QN  VAEQ GE+ GLEYVVFKT+  A  S+++ VFR  PSEVL+N++ L Q QV +LK  GN GPLV+P
Sbjct:  449 QNPAVAEQGGEQ-GLEYVVFKTHHNAVSSYIKDVFRVIPSEVLSNSYNLGQSQVRQLKYQGNSGPLVNP 516

[Close]

BLAST of GH720227 vs. SwissProt
Match: GLUB5_ORYSJ (Glutelin type-B 5 OS=Oryza sativa subsp. japonica GN=GLUB5 PE=2 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 5.176e-7
Identity = 33/82 (40.24%), Postives = 49/82 (59.76%), Query Frame = 3

Query:    3 QNFVVAEQAGEEEGLEYVVFKTNDRAAVSHV---QQVFRATPSEVLANAFGLRQRQVTELKLSGNRGP---LVHPRSQSQSH 230
            Q++VV ++A E EG +Y+ FKTN  + VSH+     +FRA P +V+ANA+ + + Q   LK   NRG       PR Q Q++
Sbjct:  409 QHYVVLKKA-EHEGCQYISFKTNANSMVSHLAGKNSIFRAMPVDVIANAYRISREQARSLK--NNRGEELGAFTPRYQQQTY 487

The following BLAST results are available for this feature:

BLAST of GH720227 vs. TrEMBL
BLAST of GH720227 vs. SwissProt

BLAST of GH720227 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q43671_VICFA	3.823e-32	90.79	Storage protein OS=Vicia faba var. minor PE=4 SV=1	[more]
Q41703_VICSA	2.739e-30	90.67	Legumin B OS=Vicia sativa PE=2 SV=1	[more]
O24294_PEA	1.191e-25	80.56	Legumin (Minor small) OS=Pisum sativum GN=legS PE=...	[more]
Q43673_VICFA	3.465e-25	76.00	Legumin; legumin-related high molecular weight pol...	[more]
B5U8K5_LOTJA	4.684e-22	72.37	Legumin storage protein 3 (Fragment) OS=Lotus japo...	[more]
B5U8K2_LOTJA	4.684e-22	72.37	Legumin storage protein 3 OS=Lotus japonicus GN=ll...	[more]
B5U8K1_LOTJA	4.684e-22	72.37	Legumin storage protein 2 OS=Lotus japonicus GN=ll...	[more]
Q9SB11_SOYBN	4.103e-18	64.00	Glycinin OS=Glycine max GN=A5A4B3 PE=2 SV=1	[more]
Q43452_SOYBN	4.103e-18	64.00	Glycinin OS=Glycine max GN=Gy4 PE=3 SV=1	[more]
Q39921_GLYSO	4.103e-18	64.00	A5A4B3 subunit OS=Glycine soja GN=glycinin Gy4 PE=...	[more]

BLAST of GH720227 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
LEGJ_PEA	1.069e-36	100.00	Legumin J OS=Pisum sativum GN=LEGJ PE=1 SV=1	[more]
LEGK_PEA	3.440e-35	96.05	Legumin K (Fragment) OS=Pisum sativum GN=LEGK PE=3...	[more]
LEGB6_VICFA	2.230e-34	93.42	Legumin type B (Fragment) OS=Vicia faba GN=LEB6 PE...	[more]
LEGB4_VICFA	2.230e-34	93.42	Legumin type B OS=Vicia faba GN=LEB4 PE=3 SV=1	[more]
LEGB2_VICFA	2.230e-34	93.42	Legumin type B (Fragment) OS=Vicia faba GN=LEB2 PE...	[more]
LEGB7_VICFA	2.912e-34	93.42	Legumin type B (Fragment) OS=Vicia faba GN=LEB7 PE...	[more]
LEGB_PEA	5.881e-27	80.56	Legumin B (Fragment) OS=Pisum sativum GN=LEGB PE=2...	[more]
GLYG4_SOYBN	2.026e-19	64.00	Glycinin G4 OS=Glycine max GN=GY4 PE=1 SV=1	[more]
GLYG5_SOYBN	3.028e-15	59.42	Glycinin OS=Glycine max PE=1 SV=1	[more]
GLUB5_ORYSJ	5.176e-7	40.24	Glutelin type-B 5 OS=Oryza sativa subsp. japonica ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006044	11-S seed storage protein, plant	PRINTS	PR00439	11SGLOBULIN	coord: 13..31 score: 1.9e-12coord: 32..49 score: 1.9
IPR011051	Cupin, RmlC-type	SUPERFAMILY	51182	RmlC-like cupins	coord: 1..69 score: 3.2
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	no description	coord: 1..74 score: 9.8
None	No IPR available	SEG	seg	seg	coord: 15..31 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720227 ID=GH720227; Name=GH720227; organism=Pisum sativum; type=EST; length=426bp
CGCAAAACTTTGTGGTGGCGGAACAAGCTGGGGAGGAAGAAGGATTAGAG
TATGTTGTGTTCAAGACAAATGACAGAGCTGCTGTTAGCCACGTACAACA
GGTGTTTAGGGCCACTCCTTCAGAGGTTCTTGCAAATGCTTTTGGTCTTC
GTCAACGCCAAGTCACGGAGTTAAAGCTCAGTGGAAACCGTGGCCCTCTG
GTTCACCCTCGGTCTCAATCTCAATCTCATTGAGATGATGCTATGCATAA
TGCAATACAATAACAAGATGGCCATCTTGTCTTGAATAATAATGATAAAA
TAAATTTTGAATGAACTGTAGTAAAAAATTTCACTTCCTATAATAAAACA
ATAAAGTATCGCCCTACTACCCTGATCTTACTCTGAATTTGTATGCATGT
AAAGAGGGGTGAATAACACTGGTTTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0045735	nutrient reservoir activity

Vocabulary: **INTERPRO**
Term	Definition
IPR006044	11S_seedstore_pln
IPR011051	RmlC_Cupin_sf
IPR014710	RmlC-like_jellyroll

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