GH720524

EST Overview


Name	GH720524
Unique Name	GH720524
Type	EST
Organism	Pisum sativum (pea)
Sequence length	444

Libraries


Library Name	Type
LIBEST_024271 552NI	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of GH720524 vs. TrEMBL
Match: B9HZ01_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_568293 PE=4 SV=1)

HSP 1 Score: 206.453 bits (524), Expect = 7.399e-52
Identity = 95/143 (66.43%), Postives = 113/143 (79.02%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPAPGFESYGESTSYPAKSTIKGDSSDPSEGAHVNLQTLYYQGPEINLENMNWRVINGPFISVWLHNVP 429
            AV+AI+RGH   LDVATI QG+T+FFS+LM AWGL+ADIDIESEKYRWMGSARLDFY L R+  LRQY+GR+SFVPAPGFE+YGE T Y  + T    S +P +  HV  +   YQGP+++L N+ WR INGPFISVWLHNVP
Sbjct:  233 AVVAIIRGHKCSLDVATILQGDTKFFSVLMFAWGLVADIDIESEKYRWMGSARLDFYGLQRMLCLRQYSGRISFVPAPGFEAYGEPTRYNGEFTSTQSSINPGQEQHVKAEQYSYQGPDVDLTNLEWRTINGPFISVWLHNVP 375

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BLAST of GH720524 vs. TrEMBL
Match: B9SFA5_RICCO (Sphingosine kinase, putative OS=Ricinus communis GN=RCOM_1095760 PE=4 SV=1)

HSP 1 Score: 199.904 bits (507), Expect = 6.926e-50
Identity = 95/143 (66.43%), Postives = 116/143 (81.12%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPAPGFESYGESTSYPAKSTIKGDSSDPSEGAHVNLQTLYYQGPEINLENMNWRVINGPFISVWLHNVP 429
            AVLAI+RGH   LDVATI QGET+FFS+LML+WGL+ADIDIESEKYRWMGSAR+DFYA+ R+F+LR YNG +SFVPAPGFE+YG  TSY A+ST K     P +  H       YQGP++NL N++WR+I+GPF+S+WLHNVP
Sbjct:  228 AVLAIIRGHKCSLDVATILQGETKFFSVLMLSWGLVADIDIESEKYRWMGSARIDFYAVQRIFHLRHYNGCISFVPAPGFETYGVPTSYNAESTSK--QEQPLKTQH------GYQGPDVNLVNLDWRMISGPFVSIWLHNVP 362

[Close]

BLAST of GH720524 vs. TrEMBL
Match: Q8L7L1_ARATH (At4g21540 OS=Arabidopsis thaliana GN=At4g21540 PE=2 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 1.265e-43
Identity = 85/143 (59.44%), Postives = 104/143 (72.73%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPAPGFESYGESTSYPAKSTIKGDSSDPSEGAHVNLQTLYYQGPEINLENMNWRVINGPFISVWLHNVP 429
            A ++I+RG +R LDVATI+QG T+FFS+LMLAWGL+ADIDIESEK+RWMGSAR D Y L R+  LRQY+GR+ FVPAPGFESYG+  S        G             +TL YQGP+  LEN++WR + GPF+SVWLHNVP
Sbjct:  237 ATISIIRGRTRSLDVATISQGTTKFFSVLMLAWGLVADIDIESEKFRWMGSARFDIYGLQRIICLRQYHGRILFVPAPGFESYGQRASCSIDKEPSGSD-----------KTLVYQGPDSKLENLDWREMKGPFVSVWLHNVP 368

[Close]

BLAST of GH720524 vs. TrEMBL
Match: Q2V3G3_ARATH (Putative uncharacterized protein At4g21540.4 OS=Arabidopsis thaliana GN=At4g21540 PE=4 SV=1)

HSP 1 Score: 179.104 bits (453), Expect = 1.265e-43
Identity = 85/143 (59.44%), Postives = 104/143 (72.73%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPAPGFESYGESTSYPAKSTIKGDSSDPSEGAHVNLQTLYYQGPEINLENMNWRVINGPFISVWLHNVP 429
            A ++I+RG +R LDVATI+QG T+FFS+LMLAWGL+ADIDIESEK+RWMGSAR D Y L R+  LRQY+GR+ FVPAPGFESYG+  S        G             +TL YQGP+  LEN++WR + GPF+SVWLHNVP
Sbjct:   27 ATISIIRGRTRSLDVATISQGTTKFFSVLMLAWGLVADIDIESEKFRWMGSARFDIYGLQRIICLRQYHGRILFVPAPGFESYGQRASCSIDKEPSGSD-----------KTLVYQGPDSKLENLDWREMKGPFVSVWLHNVP 158

[Close]

BLAST of GH720524 vs. TrEMBL
Match: C5XBK4_SORBI (Putative uncharacterized protein Sb02g036410 OS=Sorghum bicolor GN=Sb02g036410 PE=4 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 3.681e-43
Identity = 84/145 (57.93%), Postives = 103/145 (71.03%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPAPGFESYGESTSYPAKSTIKGDSSDPSE--GAHVNLQTLYYQGPEINLENMNWRVINGPFISVWLHNVP 429
            AV AI+RGH R LDV ++ QG+TRFFS+LML WGL+AD+DIESEKYRWMGSARLDFY LLR+ NLR+YNGR+ FVPAPG+E  G+       S   G S+   E      N +T  Y GP +   ++ WR +NGPF+SVWL NVP
Sbjct:  238 AVFAIIRGHKRALDVTSVVQGKTRFFSVLMLTWGLVADVDIESEKYRWMGSARLDFYLLLRVLNLRRYNGRILFVPAPGYEEVGDPVDQTTGSETNGFSTGIQEDVATDSNGETRGYVGPSVKESDLRWRSLNGPFVSVWLGNVP 382

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BLAST of GH720524 vs. TrEMBL
Match: C0PEN7_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 3.681e-43
Identity = 83/143 (58.04%), Postives = 104/143 (72.73%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPAPGFESYGESTSYPAKSTIKGDSSDPSEGAHVNLQTLYYQGPEINLENMNWRVINGPFISVWLHNVP 429
            AV AI+RGH R LDV ++ QG TRFFS+LML WGL+AD+DIESEKYRWMGSARL+FY LLR+ NLR+YNGR+ FVPAPG+E  G+    P + T   +++  S     N +T  Y GP I   ++ WR +NGPF+SVWL NVP
Sbjct:   83 AVFAIIRGHKRALDVTSVVQGNTRFFSVLMLTWGLVADVDIESEKYRWMGSARLEFYLLLRMLNLRRYNGRILFVPAPGYEEVGD----PVEQTTGHETNGVSGATDKNGETCGYVGPSIKEADLRWRSLNGPFVSVWLGNVP 221

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BLAST of GH720524 vs. TrEMBL
Match: D7MES6_ARALY (D-erythro-sphingosine kinase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_492730 PE=4 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 1.827e-42
Identity = 82/143 (57.34%), Postives = 102/143 (71.33%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPAPGFESYGESTSYPAKSTIKGDSSDPSEGAHVNLQTLYYQGPEINLENMNWRVINGPFISVWLHNVP 429
            A ++I+RG +R LDVATI+QG T+FFS+LMLAWGL+ADIDIESEK+RWMG AR D Y L R+  LRQY GR+ FVPAPGFESYG+  S      + G             + L YQGP+  LE+++WR + GPF+SVWLHNVP
Sbjct: 1025 ATISIIRGRTRSLDVATISQGTTKFFSVLMLAWGLVADIDIESEKFRWMGGARFDIYGLQRIICLRQYYGRILFVPAPGFESYGQRASCSVDKELSGSD-----------KALVYQGPDTKLEDLDWREMKGPFVSVWLHNVP 1156

HSP 2 Score: 173.326 bits (438), Expect = 6.942e-42
Identity = 87/149 (58.39%), Postives = 109/149 (73.15%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFY------ALLRLFNLRQYNGRVSFVPAPGFESYGESTSYPAKSTIKGDSSDPSEGAHVNLQTLYYQGPEINLENMNWRVINGPFISVWLHNVP 429
            A ++I+RGH R +DVATI+QG T+FFS+LMLAWGLIADIDIESEK+RWMGSAR+DFY      AL R+  LRQYNGR+ F+PAPGFESYG+    PA  ++  +         V+ + L YQGPE   E++ WR I GPF++VWLHNVP
Sbjct:  506 ATISIIRGHKRFVDVATISQGNTKFFSVLMLAWGLIADIDIESEKFRWMGSARIDFYVLVKLLALQRIICLRQYNGRILFLPAPGFESYGQ----PASCSLYKEPP-------VSDKELGYQGPETKFEDLEWREIKGPFVTVWLHNVP 643

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BLAST of GH720524 vs. TrEMBL
Match: B6TYH8_MAIZE (D-erythro-sphingosine kinase/ diacylglycerol kinase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 7.675e-41
Identity = 84/148 (56.76%), Postives = 103/148 (69.59%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPAPGFESYG----ESTSYPAKSTIKGDSSDPSEGAHVNLQTLYYQGP-EINLENMNWRVINGPFISVWLHNVP 429
            AV AI+RGH R LDV ++ QG+ RFFS+LML WGL+AD+DIESEKYRWMGSARLDFY LLR+ NLR+YNGR+ FVPAPG+E  G    ++T Y       G   D   G+  N +T  Y GP  I    + WR ++GPF+SVWL NVP
Sbjct:  237 AVFAIIRGHKRALDVTSVVQGKARFFSVLMLTWGLVADVDIESEKYRWMGSARLDFYLLLRILNLRRYNGRILFVPAPGYEEVGDPVDQATGYETNGFSTGFQDDGRTGS--NGETCGYVGPSSIKEAGLRWRSLDGPFVSVWLGNVP 382

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BLAST of GH720524 vs. TrEMBL
Match: O65419_ARATH (Putative uncharacterized protein AT4g21540 OS=Arabidopsis thaliana GN=F18E5.160 PE=4 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 4.975e-40
Identity = 83/161 (51.55%), Postives = 108/161 (67.08%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFY------------------ALLRLFNLRQYNGRVSFVPAPGFESYGESTSYPAKSTIKGDSSDPSEGAHVNLQTLYYQGPEINLENMNWRVINGPFISVWLHNVP 429
            A ++I+RGH R +DVATI QG T+FFS+LMLAWGLIADIDIESEK+RWMGSAR+DFY                  AL R+  LR+YNGR+ F+PAPGFE YG+    PA  ++        +  HV+ + + YQGPE   E++ WR + GPF+++WLHNVP
Sbjct:  496 ATISIIRGHKRSVDVATIAQGNTKFFSVLMLAWGLIADIDIESEKFRWMGSARIDFYVCLVDKFDNYCIAVVKLLALQRIICLRRYNGRILFLPAPGFEGYGQ----PASCSL-------YQEPHVSDKEVGYQGPETKFEDLEWREMKGPFVTIWLHNVP 645

HSP 2 Score: 154.451 bits (389), Expect = 3.337e-36
Identity = 82/172 (47.67%), Postives = 98/172 (56.98%), Query Frame = 1

Query:   22 GHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALL------------------------------------RLFNLRQYNGRVSFVPAPGFESYGESTSYPAKSTIKGDSSDPSEGAHVNLQTLYYQGPEINLENMNWRVINGPFISVWLHNVP 429
            G +R LDVATI+QG T+FFS+LMLAWGL+ADIDIESEK+RWMGSAR D Y  L                                    R+  LRQY+GR+ FVPAPGFESYG+  S        G             +TL YQGP+  LEN++WR + GPF+SVWLHNVP
Sbjct: 1004 GRTRSLDVATISQGTTKFFSVLMLAWGLVADIDIESEKFRWMGSARFDIYVCLVEHLKIFCNACFNMRYSTKLLVEVEQKKKKKEIGLQRIICLRQYHGRILFVPAPGFESYGQRASCSIDKEPSGSD-----------KTLVYQGPDSKLENLDWREMKGPFVSVWLHNVP 1164

[Close]

BLAST of GH720524 vs. TrEMBL
Match: B8AAF6_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_03990 PE=4 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 1.225e-38
Identity = 72/142 (50.70%), Postives = 97/142 (68.31%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPAPGFESYGESTSYPAKSTIKGDSSDPSEGAHVNLQTLYYQGPEINLENMNWRVINGPFISVWLHNV 426
            AV AI+RGHS+ LDV TI QG  +FFS+L + WGL+ADIDIESEKYRWMGSAR DFYAL+R+ NLR+Y G + +VPAPG+E+YG+        T++           +      YQGP +  +   WR ++GPF+S+W++NV
Sbjct:  307 AVFAIIRGHSQSLDVCTIVQGRKKFFSVLNMTWGLVADIDIESEKYRWMGSARFDFYALVRIMNLRKYYGSIQYVPAPGYEAYGDVVKQVENCTVECQE-------QIGKSLCSYQGPSVEFQGSEWRSLDGPFVSIWINNV 441

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BLAST of GH720524 vs. SwissProt
Match: SPHKA_DICDI (Sphingosine kinase A OS=Dictyostelium discoideum GN=sgkA PE=2 SV=2)

HSP 1 Score: 84.7297 bits (208), Expect = 1.865e-16
Identity = 38/75 (50.67%), Postives = 56/75 (74.67%), Query Frame = 1

Query:    7 LAILRGHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPA 231
            LA++RG ++ LDV+ I QG+ ++ SIL L WG+++D+DIESEKYR +G  RL   A LR+ NLR Y G++ ++PA
Sbjct:  295 LAVIRGFTKPLDVSVIQQGDKKWCSILSLTWGIVSDVDIESEKYRALGDVRLILGAALRILNLRIYRGKIWYLPA 369

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BLAST of GH720524 vs. SwissProt
Match: SPHK2_MOUSE (Sphingosine kinase 2 OS=Mus musculus GN=Sphk2 PE=1 SV=2)

HSP 1 Score: 68.5514 bits (166), Expect = 1.383e-11
Identity = 34/76 (44.74%), Postives = 51/76 (67.11%), Query Frame = 1

Query:    7 LAILRGHSRQLDVATITQGE-TRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPA 231
            L + RG S  LD+ ++T    +R FS L +AWG ++D+DI SE++R +GSAR    A+L L +L  Y GR+S++PA
Sbjct:  271 LLLCRGGSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLASLHTYRGRLSYLPA 346

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BLAST of GH720524 vs. SwissProt
Match: SPHKB_DICDI (Sphingosine kinase B OS=Dictyostelium discoideum GN=sgkB PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 5.257e-11
Identity = 31/55 (56.36%), Postives = 41/55 (74.55%), Query Frame = 1

Query:   67 TRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPA 231
            T++ SIL L WGL++D+DIESEKYR +G  RL   A +R+ NLR Y G+V F+PA
Sbjct:  444 TKWCSILSLTWGLVSDVDIESEKYRSLGDLRLIIGAAVRILNLRIYRGKVYFLPA 498

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BLAST of GH720524 vs. SwissProt
Match: SPHK2_HUMAN (Sphingosine kinase 2 OS=Homo sapiens GN=SPHK2 PE=1 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 6.866e-11
Identity = 32/76 (42.11%), Postives = 49/76 (64.47%), Query Frame = 1

Query:    7 LAILRGHSRQLDVATITQGE-TRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPA 231
            L + RG    LD+ ++T    +R FS L +AWG ++D+DI+SE++R +GSAR     +L L  L  Y GR+S++PA
Sbjct:  306 LLLCRGGGHPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERFRALGSARFTLGTVLGLATLHTYRGRLSYLPA 381

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BLAST of GH720524 vs. SwissProt
Match: SPHK1_HUMAN (Sphingosine kinase 1 OS=Homo sapiens GN=SPHK1 PE=1 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.998e-10
Identity = 30/58 (51.72%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   55 TQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVP 228
            T    R FS+L LAWG IAD+D+ESEKYR +G  R      LRL  LR Y GR++++P
Sbjct:  157 TASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLP 214

[Close]

BLAST of GH720524 vs. SwissProt
Match: SPHK1_MOUSE (Sphingosine kinase 1 OS=Mus musculus GN=Sphk1 PE=1 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.096e-8
Identity = 25/58 (43.10%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   55 TQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVP 228
            T    R +S+L L+WG +AD+D+ESEKYR +G  R       RL +LR Y G+++++P
Sbjct:  157 TASGLRLYSVLSLSWGFVADVDLESEKYRRLGEIRFTVGTFFRLASLRIYQGQLAYLP 214

[Close]

BLAST of GH720524 vs. SwissProt
Match: SPHK1_RAT (Sphingosine kinase 1 OS=Rattus norvegicus GN=Sphk1 PE=1 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 3.189e-8
Identity = 24/58 (41.38%), Postives = 38/58 (65.52%), Query Frame = 1

Query:   55 TQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVP 228
            T    + +S+L L+WG +AD+D+ESEKYR +G  R       RL +LR Y G+++++P
Sbjct:  157 TASGRQLYSVLSLSWGFVADVDLESEKYRSLGEIRFTVGTFFRLASLRIYQGQLAYLP 214

[Close]

BLAST of GH720524 vs. SwissProt
Match: CERK1_HUMAN (Ceramide kinase OS=Homo sapiens GN=CERK PE=1 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.067e-7
Identity = 30/78 (38.46%), Postives = 44/78 (56.41%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRF-FSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPA 231
            + L I+ G S  +DV+++    T   +S+ +L +G   DI  +SEK RW+G AR DF  L    +   Y G VSF+PA
Sbjct:  257 SALHIVVGDSLAMDVSSVHHNSTLLRYSVSLLGYGFYGDIIKDSEKKRWLGLARYDFSGLKTFLSHHCYEGTVSFLPA 334

[Close]

BLAST of GH720524 vs. SwissProt
Match: CERK1_MOUSE (Ceramide kinase OS=Mus musculus GN=Cerk PE=2 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 7.856e-7
Identity = 28/78 (35.90%), Postives = 44/78 (56.41%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRF-FSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPA 231
            + L I+ G S  +DV+++    T   +S+ +L +G   D+  +SEK RWMG  R DF  L    + + Y G +SF+PA
Sbjct:  257 SALHIIIGDSLAIDVSSVHYHNTLLRYSVSLLGYGFYGDLIKDSEKKRWMGLVRYDFSGLKTFLSHQYYEGTLSFLPA 334

[Close]

BLAST of GH720524 vs. TAIR peptide
Match: AT4G21540.2 (| Symbols: SPHK1 | sphingosine kinase 1 | chr4:11460703-11462356 FORWARD LENGTH=275)

HSP 1 Score: 179.104 bits (453), Expect = 8.664e-46
Identity = 85/143 (59.44%), Postives = 104/143 (72.73%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPAPGFESYGESTSYPAKSTIKGDSSDPSEGAHVNLQTLYYQGPEINLENMNWRVINGPFISVWLHNVP 429
            A ++I+RG +R LDVATI+QG T+FFS+LMLAWGL+ADIDIESEK+RWMGSAR D Y L R+  LRQY+GR+ FVPAPGFESYG+  S        G             +TL YQGP+  LEN++WR + GPF+SVWLHNVP
Sbjct:   27 ATISIIRGRTRSLDVATISQGTTKFFSVLMLAWGLVADIDIESEKFRWMGSARFDIYGLQRIICLRQYHGRILFVPAPGFESYGQRASCSIDKEPSGSD-----------KTLVYQGPDSKLENLDWREMKGPFVSVWLHNVP 158

[Close]

BLAST of GH720524 vs. TAIR peptide
Match: AT4G21540.1 (| Symbols: SPHK1 | sphingosine kinase 1 | chr4:11459609-11462356 FORWARD LENGTH=485)

HSP 1 Score: 179.104 bits (453), Expect = 8.664e-46
Identity = 85/143 (59.44%), Postives = 104/143 (72.73%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPAPGFESYGESTSYPAKSTIKGDSSDPSEGAHVNLQTLYYQGPEINLENMNWRVINGPFISVWLHNVP 429
            A ++I+RG +R LDVATI+QG T+FFS+LMLAWGL+ADIDIESEK+RWMGSAR D Y L R+  LRQY+GR+ FVPAPGFESYG+  S        G             +TL YQGP+  LEN++WR + GPF+SVWLHNVP
Sbjct:  237 ATISIIRGRTRSLDVATISQGTTKFFSVLMLAWGLVADIDIESEKFRWMGSARFDIYGLQRIICLRQYHGRILFVPAPGFESYGQRASCSIDKEPSGSD-----------KTLVYQGPDSKLENLDWREMKGPFVSVWLHNVP 368

[Close]

BLAST of GH720524 vs. TAIR peptide
Match: AT4G21534.1 (| Symbols: | Diacylglycerol kinase family protein | chr4:11456230-11458820 FORWARD LENGTH=481)

HSP 1 Score: 178.333 bits (451), Expect = 1.478e-45
Identity = 83/143 (58.04%), Postives = 108/143 (75.52%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSARLDFYALLRLFNLRQYNGRVSFVPAPGFESYGESTSYPAKSTIKGDSSDPSEGAHVNLQTLYYQGPEINLENMNWRVINGPFISVWLHNVP 429
            A ++I+RGH R +DVATI QG T+FFS+LMLAWGLIADIDIESEK+RWMGSAR+DFYAL R+  LR+YNGR+ F+PAPGFE YG+    PA  ++        +  HV+ + + YQGPE   E++ WR + GPF+++WLHNVP
Sbjct:  232 ATISIIRGHKRSVDVATIAQGNTKFFSVLMLAWGLIADIDIESEKFRWMGSARIDFYALQRIICLRRYNGRILFLPAPGFEGYGQ----PASCSL-------YQEPHVSDKEVGYQGPETKFEDLEWREMKGPFVTIWLHNVP 363

[Close]

BLAST of GH720524 vs. TAIR peptide
Match: AT4G21540.3 (| Symbols: SPHK1 | sphingosine kinase 1 | chr4:11459609-11461654 FORWARD LENGTH=320)

HSP 1 Score: 86.6557 bits (213), Expect = 3.811e-21
Identity = 38/52 (73.08%), Postives = 48/52 (92.31%), Query Frame = 1

Query:    1 AVLAILRGHSRQLDVATITQGETRFFSILMLAWGLIADIDIESEKYRWMGSA 156
            A ++I+RG +R LDVATI+QG T+FFS+LMLAWGL+ADIDIESEK+RWMGS+
Sbjct:  237 ATISIIRGRTRSLDVATISQGTTKFFSVLMLAWGLVADIDIESEKFRWMGSS 288

HSP 2 Score: 31.187 bits (69), Expect = 3.811e-21
Identity = 14/36 (38.89%), Postives = 19/36 (52.78%), Query Frame = 3

Query:  159 FRFLCSP*IIQFEAIQWTRFVCACTWV*VLWGVYQL 266
            FR++ S      + I WT  +CA +WV  LW   QL
Sbjct:  282 FRWMGSSEDNMLKTIPWTNSICASSWVRKLWATSQL 317

The following BLAST results are available for this feature:

BLAST of GH720524 vs. TrEMBL
BLAST of GH720524 vs. SwissProt
BLAST of GH720524 vs. TAIR peptide

BLAST of GH720524 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9HZ01_POPTR	7.399e-52	66.43	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SFA5_RICCO	6.926e-50	66.43	Sphingosine kinase, putative OS=Ricinus communis G...	[more]
Q8L7L1_ARATH	1.265e-43	59.44	At4g21540 OS=Arabidopsis thaliana GN=At4g21540 PE=...	[more]
Q2V3G3_ARATH	1.265e-43	59.44	Putative uncharacterized protein At4g21540.4 OS=Ar...	[more]
C5XBK4_SORBI	3.681e-43	57.93	Putative uncharacterized protein Sb02g036410 OS=So...	[more]
C0PEN7_MAIZE	3.681e-43	58.04	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
D7MES6_ARALY	1.827e-42	57.34	D-erythro-sphingosine kinase OS=Arabidopsis lyrata...	[more]
B6TYH8_MAIZE	7.675e-41	56.76	D-erythro-sphingosine kinase/ diacylglycerol kinas...	[more]
O65419_ARATH	4.975e-40	51.55	Putative uncharacterized protein AT4g21540 OS=Arab...	[more]
B8AAF6_ORYSI	1.225e-38	50.70	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of GH720524 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 9


Match Name	E-value	Identity	Description
SPHKA_DICDI	1.865e-16	50.67	Sphingosine kinase A OS=Dictyostelium discoideum G...	[more]
SPHK2_MOUSE	1.383e-11	44.74	Sphingosine kinase 2 OS=Mus musculus GN=Sphk2 PE=1...	[more]
SPHKB_DICDI	5.257e-11	56.36	Sphingosine kinase B OS=Dictyostelium discoideum G...	[more]
SPHK2_HUMAN	6.866e-11	42.11	Sphingosine kinase 2 OS=Homo sapiens GN=SPHK2 PE=1...	[more]
SPHK1_HUMAN	1.998e-10	51.72	Sphingosine kinase 1 OS=Homo sapiens GN=SPHK1 PE=1...	[more]
SPHK1_MOUSE	1.096e-8	43.10	Sphingosine kinase 1 OS=Mus musculus GN=Sphk1 PE=1...	[more]
SPHK1_RAT	3.189e-8	41.38	Sphingosine kinase 1 OS=Rattus norvegicus GN=Sphk1...	[more]
CERK1_HUMAN	2.067e-7	38.46	Ceramide kinase OS=Homo sapiens GN=CERK PE=1 SV=1	[more]
CERK1_MOUSE	7.856e-7	35.90	Ceramide kinase OS=Mus musculus GN=Cerk PE=2 SV=1	[more]

BLAST of GH720524 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT4G21540.2	8.664e-46	59.44	\| Symbols: SPHK1 \| sphingosine kinase 1 \| chr4:114...	[more]
AT4G21540.1	8.664e-46	59.44	\| Symbols: SPHK1 \| sphingosine kinase 1 \| chr4:114...	[more]
AT4G21534.1	1.478e-45	58.04	\| Symbols: \| Diacylglycerol kinase family protein...	[more]
AT4G21540.3	3.811e-21	73.08	\| Symbols: SPHK1 \| sphingosine kinase 1 \| chr4:114...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR12358	SPHINGOSINE KINASE	coord: 1..143 score: 3.5
None	No IPR available	PANTHER	PTHR12358:SF10	SPHINGOSINE KINASE-RELATED	coord: 1..143 score: 3.5

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720524 ID=GH720524; Name=GH720524; organism=Pisum sativum; type=EST; length=444bp
GCCGTTCTTGCTATTCTACGAGGCCATTCGCGACAGCTTGATGTGGCTAC
CATCACACAAGGAGAAACCAGATTTTTCAGTATACTAATGCTTGCATGGG
GTCTAATTGCTGATATTGACATCGAGTCTGAAAAGTATCGGTGGATGGGA
AGTGCTCGTTTAGATTTTTATGCTCTCCTTAGATTATTCAATTTGAGGCA
ATACAATGGACGCGTTTCGTTTGTGCCTGCACCTGGGTTTGAGTCTTATG
GGGAGTCTACCAGTTACCCTGCCAAATCCACTATCAAAGGTGACAGCAGT
GATCCAAGTGAAGGAGCACATGTTAATTTGCAAACACTTTATTATCAAGG
ACCTGAAATAAACTTGGAAAATATGAATTGGAGAGTTATAAACGGACCCT
TTATTAGTGTCTGGCTTCACAATGTACCTCGGCCGCGACCACGC

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