CD860311

EST Overview


Name	CD860311
Unique Name	CD860311
Type	EST
Organism	Pisum sativum (pea)
Sequence length	407

Libraries


Library Name	Type
LIBEST_014003 TE	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of CD860311 vs. TrEMBL
Match: Q9ZP43_PHAVU (Alpha-amylase OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 251.906 bits (642), Expect = 1.530e-65
Identity = 114/135 (84.44%), Postives = 127/135 (94.07%), Query Frame = 2

Query:    2 SLKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLNPQ 406
            SLKN++PD+ANAGITHVWLPPPSQSV P+GYLPGRLYDLDAS+YG+KDELKSLIAA ++KGIK +ADIVINHRTAERKD RGIYCIFEGGTPD++LDWGPSFICK DTTYSDG GN+D+GE Y AAPDIDHLNPQ
Sbjct:   43 SLKNSVPDIANAGITHVWLPPPSQSVSPEGYLPGRLYDLDASRYGSKDELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDARLDWGPSFICKDDTTYSDGTGNNDSGESYDAAPDIDHLNPQ 177

[Close]

BLAST of CD860311 vs. TrEMBL
Match: C6T9Z1_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 1.999e-65
Identity = 117/135 (86.67%), Postives = 124/135 (91.85%), Query Frame = 2

Query:    2 SLKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLNPQ 406
            SLKNTIPDLANAGITHVWLPPPSQSV P+GYLPGRLYDLDASKYGTKD+LKSLIAA +DKGIK +ADIVINHRTAERKD RGIYCIFEGGTPD++LDWGPSFICK D TYSDG GN D+GE Y  APDIDHLNPQ
Sbjct:   43 SLKNTIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDASKYGTKDQLKSLIAAFHDKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDARLDWGPSFICKDDNTYSDGTGNLDSGEPYDPAPDIDHLNPQ 177

[Close]

BLAST of CD860311 vs. TrEMBL
Match: Q7X9T1_PHAAN (Alpha-amylase OS=Phaseolus angularis PE=2 SV=1)

HSP 1 Score: 247.669 bits (631), Expect = 2.886e-64
Identity = 114/135 (84.44%), Postives = 125/135 (92.59%), Query Frame = 2

Query:    2 SLKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLNPQ 406
            SLKN+IPDLANAGITHVWLPPPSQSV P+GYLPGRLYDLDAS+YG+K+ELKSLIAA  +KGIK +ADIVINHRTAERKD RGIYCIFEGGTPDS+LDWGPSFIC+ DT YSDG GN+D+GE Y AAPDIDHLNPQ
Sbjct:   43 SLKNSIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDASRYGSKNELKSLIAAFYEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRLDWGPSFICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQ 177

[Close]

BLAST of CD860311 vs. TrEMBL
Match: B2KIQ7_MANES (Alpha-amylase (Fragment) OS=Manihot esculenta PE=4 SV=1)

HSP 1 Score: 242.276 bits (617), Expect = 1.213e-62
Identity = 110/135 (81.48%), Postives = 122/135 (90.37%), Query Frame = 2

Query:    2 SLKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLNPQ 406
            SLKN IPDLANAG+THVWLPPPSQS  PQGYLPGRLYDL+ASKYGT+DEL SLI + + KGIKS+ADIVINHRTAE+KDDRGIYCIFEGGTPD  LDWGPSFIC+ DT YSDG+GN DTGED++ APDIDHLNP+
Sbjct:   17 SLKNMIPDLANAGVTHVWLPPPSQSAAPQGYLPGRLYDLNASKYGTQDELVSLIDSFHQKGIKSLADIVINHRTAEKKDDRGIYCIFEGGTPDGTLDWGPSFICRDDTAYSDGQGNPDTGEDFKGAPDIDHLNPR 151

[Close]

BLAST of CD860311 vs. TrEMBL
Match: Q8LP27_IPONI (Alpha-amylase OS=Ipomoea nil GN=PnAmy1 PE=2 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 6.228e-59
Identity = 105/135 (77.78%), Postives = 117/135 (86.67%), Query Frame = 2

Query:    2 SLKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLNPQ 406
            SL N+IPDLANAGITHVWLPPPS SV PQGY+PGRLYDLDASKYG K +L++L+AAL+DKGIK+VADIVINHR A+ KD RGIYCIFEGGTPD +LDWGP  ICK DT YSDG GN DTG D+  APDIDHLNP+
Sbjct:   43 SLINSIPDLANAGITHVWLPPPSHSVAPQGYMPGRLYDLDASKYGNKQQLQALVAALHDKGIKAVADIVINHRCADYKDSRGIYCIFEGGTPDDRLDWGPGLICKDDTQYSDGTGNADTGMDFGGAPDIDHLNPR 177

[Close]

BLAST of CD860311 vs. TrEMBL
Match: B9R7C6_RICCO (Pentatricopeptide repeat-containing protein, putative OS=Ricinus communis GN=RCOM_1590660 PE=3 SV=1)

HSP 1 Score: 229.565 bits (584), Expect = 8.134e-59
Identity = 105/134 (78.36%), Postives = 119/134 (88.81%), Query Frame = 2

Query:    5 LKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLNPQ 406
            LKN+I D+ANAGITHVWLPPPSQSV PQGY+PGRLYDL+AS+YGT+DELKSLI A + KGIK +ADIVINHRTAE+KD+RGI+CIFEGGT D +LDWGPSFIC  DT +SDG GN DTGEDY  APDIDHLNP+
Sbjct:   47 LKNSILDIANAGITHVWLPPPSQSVSPQGYMPGRLYDLNASRYGTQDELKSLIRAFHHKGIKCLADIVINHRTAEKKDERGIWCIFEGGTDDDRLDWGPSFICCDDTVFSDGTGNPDTGEDYPPAPDIDHLNPR 180

[Close]

BLAST of CD860311 vs. TrEMBL
Match: B9R7C5_RICCO (Alpha-amylase, putative OS=Ricinus communis GN=RCOM_1590650 PE=3 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 1.387e-58
Identity = 103/135 (76.30%), Postives = 119/135 (88.15%), Query Frame = 2

Query:    2 SLKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLNPQ 406
            SLKN +PD+A+AGITHVWLPP SQSV PQGY+PGRLYDL  SKYG +DELKSLI AL+ KGIK +ADIVINHRTAE++D+RGI+CIFEGGT D +LDWGPSFIC+ DT YSDGKGN DTG D++ APDIDHLNP+
Sbjct:   40 SLKNFVPDIASAGITHVWLPPSSQSVAPQGYMPGRLYDLSVSKYGNQDELKSLIQALHQKGIKCLADIVINHRTAEKQDERGIWCIFEGGTADDRLDWGPSFICRDDTAYSDGKGNPDTGGDFEPAPDIDHLNPR 174

[Close]

BLAST of CD860311 vs. TrEMBL
Match: B9GNW3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_644125 PE=3 SV=1)

HSP 1 Score: 224.557 bits (571), Expect = 2.617e-57
Identity = 103/135 (76.30%), Postives = 115/135 (85.19%), Query Frame = 2

Query:    2 SLKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLNPQ 406
            SLKN +PDLANAGITHVWLPP SQSV PQGY+PGRLYDL ASKYG++ ELKSLI A + KGIK +ADIVINHRTAE++D RGI+CIFEGGT D +LDWGPS IC  DT YSDGKGN DTG D+  APDIDHLNP+
Sbjct:   24 SLKNLVPDLANAGITHVWLPPASQSVAPQGYMPGRLYDLSASKYGSQGELKSLIGAFHQKGIKCLADIVINHRTAEKQDGRGIWCIFEGGTSDDRLDWGPSLICSDDTAYSDGKGNPDTGGDFGGAPDIDHLNPR 158

[Close]

BLAST of CD860311 vs. TrEMBL
Match: B9MVQ5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_918430 PE=3 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 2.215e-56
Identity = 101/136 (74.26%), Postives = 119/136 (87.50%), Query Frame = 2

Query:    2 SLKNTIPDLANAGITHVWLPPPSQSVGPQG-YLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLNPQ 406
            SLKN++PDLANAGITHVWLPP SQSV PQG Y+PGRLYDL AS+YG++DELKSLI   N KGIK +ADIVINHRTAE++D+RGI+ +FEGGT D +LDWGPSFIC  DT YSDGKGN D+G+D++ APDIDHLNP+
Sbjct:   43 SLKNSVPDLANAGITHVWLPPSSQSVAPQGRYMPGRLYDLSASRYGSQDELKSLIGDFNQKGIKCLADIVINHRTAEKQDERGIWSVFEGGTSDDRLDWGPSFICGDDTAYSDGKGNPDSGDDFEPAPDIDHLNPR 178

[Close]

BLAST of CD860311 vs. TrEMBL
Match: Q42678_CUSRE (Alpha amylase (Fragment) OS=Cuscuta reflexa GN=CUS AMY2 PE=2 SV=1)

HSP 1 Score: 218.779 bits (556), Expect = 1.436e-55
Identity = 102/133 (76.69%), Postives = 112/133 (84.21%), Query Frame = 2

Query:    2 SLKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLN 400
            SL N IPDLANAGITHVWLPP S SV PQGY+PGRLYDLDASKYG K +L+SL+ AL+ KGIK+VADIVINHR A+ KD RGIYC+FEGGT D +LDWGPS ICK DT YSDG GN DTG D+ AAPDIDHLN
Sbjct:   40 SLINLIPDLANAGITHVWLPPSSHSVSPQGYMPGRLYDLDASKYGNKQQLQSLVQALHAKGIKAVADIVINHRCADYKDSRGIYCLFEGGTSDGRLDWGPSHICKDDTQYSDGTGNPDTGMDFAAAPDIDHLN 172

[Close]

BLAST of CD860311 vs. SwissProt
Match: AMYA_VIGMU (Alpha-amylase OS=Vigna mungo GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 247.669 bits (631), Expect = 1.420e-65
Identity = 114/135 (84.44%), Postives = 125/135 (92.59%), Query Frame = 2

Query:    2 SLKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLNPQ 406
            SLKN+IPDLANAGITHVWLPPPSQSV P+GYLPGRLYDLDASKYG+K+ELKSLIAA ++KGIK +ADIVINHRTAERKD RGIYCIFEGGTPDS+ DWGPSFIC+ DT YSDG GN+D+GE Y AAPDIDHLNPQ
Sbjct:   43 SLKNSIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDASKYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRQDWGPSFICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQ 177

[Close]

BLAST of CD860311 vs. SwissProt
Match: AMY3D_ORYSJ (Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica GN=AMY1.3 PE=2 SV=2)

HSP 1 Score: 216.083 bits (549), Expect = 4.579e-56
Identity = 96/134 (71.64%), Postives = 111/134 (82.84%), Query Frame = 2

Query:    5 LKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLNPQ 406
            LK  + D+A AG+THVWLPPPS SV PQGY+PGRLYDLDASKYGT  ELKSLIAA + KG++ VAD+VINHR AE+KD RG+YC+FEGGTPD +LDWGP  IC  DT YSDG G+ DTGE + AAPDIDHLNP+
Sbjct:   47 LKGQVDDIAKAGVTHVWLPPPSHSVAPQGYMPGRLYDLDASKYGTAAELKSLIAAFHGKGVQCVADVVINHRCAEKKDARGVYCVFEGGTPDDRLDWGPGMICSDDTQYSDGTGHRDTGEGFGAAPDIDHLNPR 180

[Close]

BLAST of CD860311 vs. SwissProt
Match: AMY2_HORVU (Alpha-amylase type B isozyme OS=Hordeum vulgare GN=AMY1.2 PE=1 SV=3)

HSP 1 Score: 210.305 bits (534), Expect = 2.513e-54
Identity = 96/132 (72.73%), Postives = 109/132 (82.58%), Query Frame = 2

Query:    5 LKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLN 400
            L   + D+A AGITHVWLPP SQSV  QGY+PGRLYDLDASKYG K +LKSLI AL+ KG+K++ADIVINHRTAE KD RGIYCIFEGGTPD++LDWGP  IC+ D  Y+DG GN DTG D+ AAPDIDHLN
Sbjct:   46 LMGKVDDIAAAGITHVWLPPASQSVAEQGYMPGRLYDLDASKYGNKAQLKSLIGALHGKGVKAIADIVINHRTAEHKDGRGIYCIFEGGTPDARLDWGPHMICRDDRPYADGTGNPDTGADFGAAPDIDHLN 177

[Close]

BLAST of CD860311 vs. SwissProt
Match: AMY3B_ORYSJ (Alpha-amylase isozyme 3B OS=Oryza sativa subsp. japonica GN=AMY1.6 PE=2 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 5.598e-54
Identity = 96/132 (72.73%), Postives = 108/132 (81.82%), Query Frame = 2

Query:    5 LKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLN 400
            L   + D+A  G+THVWLPPPS SV PQGY+PGRLYDLDASKYGT  EL+SLIAA + KGIK VADIVINHR A+ KD RGIYCIFEGGTPDS+LDWGP  IC  DT YS+G+G+ DTG D+ AAPDIDHLN
Sbjct:   48 LHGHVDDIAATGVTHVWLPPPSHSVAPQGYMPGRLYDLDASKYGTGAELRSLIAAFHSKGIKCVADIVINHRCADYKDSRGIYCIFEGGTPDSRLDWGPDMICSDDTQYSNGRGHRDTGADFGAAPDIDHLN 179

[Close]

BLAST of CD860311 vs. SwissProt
Match: AMY3C_ORYSJ (Alpha-amylase isozyme 3C OS=Oryza sativa subsp. japonica GN=AMY1.7 PE=2 SV=2)

HSP 1 Score: 207.223 bits (526), Expect = 2.127e-53
Identity = 95/132 (71.97%), Postives = 108/132 (81.82%), Query Frame = 2

Query:    5 LKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLN 400
            L + + D+A  G+THVWLPPPS SV PQGY+PGRLYDLDASKYGT  EL+SLIAA + K IK VADIVINHR A+ KD RGIYCIFEGGTPDS+LDWGP  IC  DT YS+G+G+ DTG D+ AAPDIDHLN
Sbjct:   48 LHSHVDDIAATGVTHVWLPPPSHSVAPQGYMPGRLYDLDASKYGTGAELRSLIAAFHSKSIKCVADIVINHRCADYKDSRGIYCIFEGGTPDSRLDWGPDMICSDDTQYSNGRGHRDTGADFGAAPDIDHLN 179

[Close]

BLAST of CD860311 vs. SwissProt
Match: AMY3_HORVU (Alpha-amylase type B isozyme (Fragment) OS=Hordeum vulgare GN=AMY1.3 PE=2 SV=1)

HSP 1 Score: 206.838 bits (525), Expect = 2.778e-53
Identity = 95/132 (71.97%), Postives = 108/132 (81.82%), Query Frame = 2

Query:    5 LKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLN 400
            L   + D+A AGITHVWLPP SQSV  QGY+PGRLYDLDASKYG K +LKSLI AL+ KG+K++ADIVINHRTAE KD RGIYCIFEG TPD++LDWGP  IC+ D  Y+DG GN DTG D+ AAPDIDHLN
Sbjct:   46 LMGKVDDIAAAGITHVWLPPASQSVAEQGYMPGRLYDLDASKYGNKAQLKSLIGALHGKGVKAIADIVINHRTAEHKDGRGIYCIFEGVTPDARLDWGPHMICRDDRPYADGTGNPDTGADFGAAPDIDHLN 177

[Close]

BLAST of CD860311 vs. SwissProt
Match: AMY3A_ORYSJ (Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica GN=AMY1.2 PE=2 SV=2)

HSP 1 Score: 206.838 bits (525), Expect = 2.778e-53
Identity = 95/133 (71.43%), Postives = 110/133 (82.71%), Query Frame = 2

Query:    5 LKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLNP 403
            LK+ + D+A+AG+THVWLPPP+ SV PQGY+PGRLYDL+ASKYGTK ELKSLIAA + KGIK VADIV+NHR A+ KD RG+YCIF+GG P   LDWGPS IC  DT YSDG G+ DTG D+ AAPDIDHLNP
Sbjct:   50 LKDQVGDIASAGVTHVWLPPPTHSVSPQGYMPGRLYDLNASKYGTKAELKSLIAAFHAKGIKCVADIVVNHRCADDKDGRGVYCIFKGGGPRGCLDWGPSMICCDDTQYSDGTGHRDTGADFAAAPDIDHLNP 182

[Close]

BLAST of CD860311 vs. SwissProt
Match: AMY2A_ORYSJ (Alpha-amylase isozyme 2A OS=Oryza sativa subsp. japonica GN=AMY2A PE=2 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 1.056e-52
Identity = 92/132 (69.70%), Postives = 108/132 (81.82%), Query Frame = 2

Query:    5 LKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLN 400
            L   + D+  AG+THVWLPPPS SV  QGY+PGRLYDLDAS+YGT  ELKSLI+AL+ KGI+++AD+VINHR A+ KD RGIYCIFEGGTPD +LDWGP  IC+ DT +SDG GN DTG D+ AAPDIDHLN
Sbjct:   44 LMGKVDDIVAAGVTHVWLPPPSHSVSTQGYMPGRLYDLDASRYGTSMELKSLISALHGKGIQAIADVVINHRCADYKDSRGIYCIFEGGTPDGRLDWGPHMICRDDTQFSDGTGNLDTGADFAAAPDIDHLN 175

[Close]

BLAST of CD860311 vs. SwissProt
Match: AMY2A_ORYSI (Alpha-amylase isozyme 2A OS=Oryza sativa subsp. indica GN=AMYC2 PE=2 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 1.056e-52
Identity = 92/132 (69.70%), Postives = 108/132 (81.82%), Query Frame = 2

Query:    5 LKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLN 400
            L   + D+  AG+THVWLPPPS SV  QGY+PGRLYDLDAS+YGT  ELKSLI+AL+ KGI+++AD+VINHR A+ KD RGIYCIFEGGTPD +LDWGP  IC+ DT +SDG GN DTG D+ AAPDIDHLN
Sbjct:   44 LMGKVDDIVAAGVTHVWLPPPSHSVSTQGYMPGRLYDLDASRYGTSMELKSLISALHGKGIQAIADVVINHRCADYKDSRGIYCIFEGGTPDGRLDWGPHMICRDDTQFSDGTGNLDTGADFAAAPDIDHLN 175

[Close]

BLAST of CD860311 vs. SwissProt
Match: AMY1_ORYSJ (Alpha-amylase OS=Oryza sativa subsp. japonica GN=AMY1.1 PE=2 SV=2)

HSP 1 Score: 203.756 bits (517), Expect = 2.352e-52
Identity = 94/132 (71.21%), Postives = 107/132 (81.06%), Query Frame = 2

Query:    5 LKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLN 400
            L   + D+A AGITHVWLPPPS SVG QGY+PGRLYDLDASKYG + +LKSLI A + KG++ +ADIVINHRTAE KD RGIYC+FEGGTPDS+LDWGP  IC+ D  Y DG GN DTG D+ AAPDIDHLN
Sbjct:   53 LMGKVDDIAAAGITHVWLPPPSHSVGEQGYMPGRLYDLDASKYGNEAQLKSLIEAFHGKGVQVIADIVINHRTAEHKDGRGIYCLFEGGTPDSRLDWGPHMICR-DDPYGDGTGNPDTGADFAAAPDIDHLN 183

[Close]

BLAST of CD860311 vs. TAIR peptide
Match: AT4G25000.1 (| Symbols: ATAMY1, AMY1 | alpha-amylase-like | chr4:12852109-12853825 REVERSE LENGTH=423)

HSP 1 Score: 204.912 bits (520), Expect = 1.197e-53
Identity = 95/135 (70.37%), Postives = 113/135 (83.70%), Query Frame = 2

Query:    2 SLKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDHLNPQ 406
            SL N+I D+ANAGITH+WLPPPSQSV P+GYLPG+LYDL++SKYG++ ELKSLI ALN KGIK++ADIVINHRTAERKDD+  YC FEGGT D +LDW PSF+C+ D  +  G GN DTG D+  APDIDHLNP+
Sbjct:   46 SLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNSSKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHLNPR 179

[Close]

BLAST of CD860311 vs. TAIR peptide
Match: AT1G69830.1 (| Symbols: ATAMY3, AMY3 | alpha-amylase-like 3 | chr1:26288518-26293003 REVERSE LENGTH=887)

HSP 1 Score: 107.071 bits (266), Expect = 3.398e-24
Identity = 53/130 (40.77%), Postives = 82/130 (63.08%), Query Frame = 2

Query:    5 LKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDH 394
            L+    +LA+ G T +WLPPP++SV P+GY+P  LY+L+ S+YGT DELK  +   +  GIK + D V+NHR A  K+  G++ +F G     +L+W    +   D  +  G+GN  +G+++ AAP+IDH
Sbjct:  516 LQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYNLN-SRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-----RLNWDDRAVV-ADDPHFQGRGNKSSGDNFHAAPNIDH 638

[Close]

BLAST of CD860311 vs. TAIR peptide
Match: AT1G76130.1 (| Symbols: ATAMY2, AMY2 | alpha-amylase-like 2 | chr1:28561647-28563914 FORWARD LENGTH=413)

HSP 1 Score: 101.293 bits (251), Expect = 1.864e-22
Identity = 51/131 (38.93%), Postives = 78/131 (59.54%), Query Frame = 2

Query:    2 SLKNTIPDLANAGITHVWLPPPSQSVGPQGYLPGRLYDLDASKYGTKDELKSLIAALNDKGIKSVADIVINHRTAERKDDRGIYCIFEGGTPDSKLDWGPSFICKGDTTYSDGKGNDDTGEDYQAAPDIDH 394
            +L   +PD+A +G T  WLPPPSQS+ P+GYLP  LY L+ S YG++  LKSL+  +    ++++ADIVINHR    +   G+Y  ++G      L W    +    T+ + G GN  TG+++   P++DH
Sbjct:   44 NLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSLN-SAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDG----ISLPWDEHAV----TSCTGGLGNRSTGDNFNGVPNVDH 165

The following BLAST results are available for this feature:

BLAST of CD860311 vs. TrEMBL
BLAST of CD860311 vs. SwissProt
BLAST of CD860311 vs. TAIR peptide

BLAST of CD860311 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q9ZP43_PHAVU	1.530e-65	84.44	Alpha-amylase OS=Phaseolus vulgaris PE=2 SV=1	[more]
C6T9Z1_SOYBN	1.999e-65	86.67	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q7X9T1_PHAAN	2.886e-64	84.44	Alpha-amylase OS=Phaseolus angularis PE=2 SV=1	[more]
B2KIQ7_MANES	1.213e-62	81.48	Alpha-amylase (Fragment) OS=Manihot esculenta PE=4...	[more]
Q8LP27_IPONI	6.228e-59	77.78	Alpha-amylase OS=Ipomoea nil GN=PnAmy1 PE=2 SV=1	[more]
B9R7C6_RICCO	8.134e-59	78.36	Pentatricopeptide repeat-containing protein, putat...	[more]
B9R7C5_RICCO	1.387e-58	76.30	Alpha-amylase, putative OS=Ricinus communis GN=RCO...	[more]
B9GNW3_POPTR	2.617e-57	76.30	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9MVQ5_POPTR	2.215e-56	74.26	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q42678_CUSRE	1.436e-55	76.69	Alpha amylase (Fragment) OS=Cuscuta reflexa GN=CUS...	[more]

BLAST of CD860311 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
AMYA_VIGMU	1.420e-65	84.44	Alpha-amylase OS=Vigna mungo GN=AMY1.1 PE=2 SV=1	[more]
AMY3D_ORYSJ	4.579e-56	71.64	Alpha-amylase isozyme 3D OS=Oryza sativa subsp. ja...	[more]
AMY2_HORVU	2.513e-54	72.73	Alpha-amylase type B isozyme OS=Hordeum vulgare GN...	[more]
AMY3B_ORYSJ	5.598e-54	72.73	Alpha-amylase isozyme 3B OS=Oryza sativa subsp. ja...	[more]
AMY3C_ORYSJ	2.127e-53	71.97	Alpha-amylase isozyme 3C OS=Oryza sativa subsp. ja...	[more]
AMY3_HORVU	2.778e-53	71.97	Alpha-amylase type B isozyme (Fragment) OS=Hordeum...	[more]
AMY3A_ORYSJ	2.778e-53	71.43	Alpha-amylase isozyme 3A OS=Oryza sativa subsp. ja...	[more]
AMY2A_ORYSJ	1.056e-52	69.70	Alpha-amylase isozyme 2A OS=Oryza sativa subsp. ja...	[more]
AMY2A_ORYSI	1.056e-52	69.70	Alpha-amylase isozyme 2A OS=Oryza sativa subsp. in...	[more]
AMY1_ORYSJ	2.352e-52	71.21	Alpha-amylase OS=Oryza sativa subsp. japonica GN=A...	[more]

BLAST of CD860311 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G25000.1	1.197e-53	70.37	\| Symbols: ATAMY1, AMY1 \| alpha-amylase-like \| chr...	[more]
AT1G69830.1	3.398e-24	40.77	\| Symbols: ATAMY3, AMY3 \| alpha-amylase-like 3 \| c...	[more]
AT1G76130.1	1.864e-22	38.93	\| Symbols: ATAMY2, AMY2 \| alpha-amylase-like 2 \| c...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	PFAM	PF00128	Alpha-amylase	coord: 4..80 score: 3.4
IPR013781	Glycoside hydrolase, subgroup, catalytic core	GENE3D	3.20.20.80	no description	coord: 3..135 score: 1.9
IPR017853	Glycoside hydrolase, catalytic core	SUPERFAMILY	51445	(Trans)glycosidases	coord: 2..135 score: 5.6
None	No IPR available	PANTHER	PTHR10357	AMYLASE	coord: 1..135 score: 1.1
None	No IPR available	PANTHER	PTHR10357:SF9	ALPHA-AMYLASE	coord: 1..135 score: 1.1

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD860311 ID=CD860311; Name=CD860311; organism=Pisum sativum; type=EST; length=407bp
CTCTTTGAAGAACACCATTCCTGACTTAGCAAACGCCGGAATTACACATG
TTTGGCTCCCTCCTCCTTCTCAAAGTGTTGGACCTCAAGGATATCTTCCT
GGAAGGCTATATGATCTTGATGCATCAAAATATGGAACAAAAGATGAACT
GAAGTCACTAATTGCCGCTCTCAATGATAAAGGAATCAAATCTGTAGCTG
ACATAGTCATCAACCATAGAACAGCCGAGAGAAAAGATGATAGAGGAATT
TATTGCATCTTCGAAGGTGGAACTCCAGATTCAAAGCTTGATTGGGGTCC
ATCTTTCATTTGCAAAGGCGACACCACTTATTCCGATGGCAAAGGAAACG
ATGATACAGGAGAGGATTATCAAGCTGCACCTGACATTGATCATCTCAAT
CCACAAG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0043169	cation binding

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR006047	Glyco_hydro_13_cat_dom
IPR013781	Glyco_hydro_catalytic_dom
IPR017853	Glycoside_hydrolase_SF

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