GH720742

EST Overview


Name	GH720742
Unique Name	GH720742
Type	EST
Organism	Pisum sativum (pea)
Sequence length	503

Libraries


Library Name	Type
LIBEST_024272 BACI	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of GH720742 vs. TrEMBL
Match: B7FHP1_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 3.791e-48
Identity = 98/115 (85.22%), Postives = 103/115 (89.57%), Query Frame = -1

Query:    6 LGLCRVDF*RFYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 350
            LG   + F  F+TLFLE+LEDVIQGKETSISHEELVLRSR QSVSDYVVMFFRFVT   IQ RSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHI ALSDA+G+PIRVVYL
Sbjct:  131 LGYAELTFEDFFTLFLEQLEDVIQGKETSISHEELVLRSRGQSVSDYVVMFFRFVTSAEIQKRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHITALSDALGIPIRVVYL 245

HSP 2 Score: 114.005 bits (284), Expect = 4.985e-24
Identity = 61/101 (60.40%), Postives = 70/101 (69.31%), Query Frame = -2

Query:  197 RGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDFIRYSLRSWKM*FKGKRLP*VMKSLCLEA---EISLYLIMLLCF 490
            R TRGDGNCFFRGFMFSYLEH+LEAQD AEIDRIK N+ERSR ALQTLGYAELTFEDF    L   +   +GK      + L L +    +S Y++M   F
Sbjct:   84 RRTRGDGNCFFRGFMFSYLEHILEAQDSAEIDRIKANVERSRKALQTLGYAELTFEDFFTLFLEQLEDVIQGKETSISHEELVLRSRGQSVSDYVVMFFRF 184

[Close]

BLAST of GH720742 vs. TrEMBL
Match: C6TEY7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 7.149e-47
Identity = 94/115 (81.74%), Postives = 103/115 (89.57%), Query Frame = -1

Query:    6 LGLCRVDF*RFYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 350
            LG   + F  F+ LFLE+LE VIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVT  AIQ R+EFFEPFI+GLTNTTVEQFCKS+VEPMGEESDHVHI ALSDA+G+P+RVVYL
Sbjct:  134 LGYADLTFEDFFALFLEQLESVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTSAAIQKRTEFFEPFILGLTNTTVEQFCKSSVEPMGEESDHVHITALSDALGIPVRVVYL 248

HSP 2 Score: 104.375 bits (259), Expect = 3.950e-21
Identity = 53/101 (52.48%), Postives = 69/101 (68.32%), Query Frame = -2

Query:  197 RGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDFIRYSLRSWKM*FKGKRLP*VMKSLCL---EAEISLYLIMLLCF 490
            R TRGDGNCFFR FMFSYLEH+++ QD+AE+DRI+ N+E+SR ALQTLGYA+LTFEDF    L   +   +GK      + L L   +  +S Y++M   F
Sbjct:   87 RRTRGDGNCFFRSFMFSYLEHVMKCQDQAEVDRIQANVEKSRKALQTLGYADLTFEDFFALFLEQLESVIQGKETSISHEELVLRSRDQSVSDYVVMFFRF 187

[Close]

BLAST of GH720742 vs. TrEMBL
Match: B9GZ22_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_830917 PE=4 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 9.662e-44
Identity = 90/115 (78.26%), Postives = 99/115 (86.09%), Query Frame = -1

Query:    6 LGLCRVDF*RFYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 350
            LG     F  F+ LFLE+L+DV+QG ETSISHEEL+ RSRDQSVSDYVVMFFRFVT   I+ RSEFFEPF+ GLTNTTVEQFCKS+VEPMGEESDHVHI ALSDA+GVPIRVVYL
Sbjct:  125 LGYVDFTFEDFFALFLEQLDDVLQGNETSISHEELLNRSRDQSVSDYVVMFFRFVTSGEIRRRSEFFEPFVFGLTNTTVEQFCKSSVEPMGEESDHVHITALSDALGVPIRVVYL 239

HSP 2 Score: 94.7449 bits (234), Expect = 3.130e-18
Identity = 42/58 (72.41%), Postives = 48/58 (82.76%), Query Frame = -2

Query:  317 RGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDF 490
            R TRGDGNCFFR FMFSYLEH+LE QD+AE+DRIK N+E  R  LQ+LGY + TFEDF
Sbjct:   78 RRTRGDGNCFFRSFMFSYLEHILEKQDRAEVDRIKANVEECRKTLQSLGYVDFTFEDF 135

[Close]

BLAST of GH720742 vs. TrEMBL
Match: A7YFC1_POPTR (Otubain-like cysteine protease (Fragment) OS=Populus trichocarpa GN=OTU1 PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 9.662e-44
Identity = 90/115 (78.26%), Postives = 99/115 (86.09%), Query Frame = -1

Query:    6 LGLCRVDF*RFYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 350
            LG     F  F+ LFLE+L+DV+QG ETSISHEEL+ RSRDQSVSDYVVMFFRFVT   I+ RSEFFEPF+ GLTNTTVEQFCKS+VEPMGEESDHVHI ALSDA+GVPIRVVYL
Sbjct:   92 LGYVDFTFEDFFALFLEQLDDVLQGNETSISHEELLNRSRDQSVSDYVVMFFRFVTSGEIRRRSEFFEPFVFGLTNTTVEQFCKSSVEPMGEESDHVHITALSDALGVPIRVVYL 206

HSP 2 Score: 94.7449 bits (234), Expect = 3.130e-18
Identity = 42/58 (72.41%), Postives = 48/58 (82.76%), Query Frame = -2

Query:  317 RGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDF 490
            R TRGDGNCFFR FMFSYLEH+LE QD+AE+DRIK N+E  R  LQ+LGY + TFEDF
Sbjct:   45 RRTRGDGNCFFRSFMFSYLEHILEKQDRAEVDRIKANVEECRKTLQSLGYVDFTFEDF 102

[Close]

BLAST of GH720742 vs. TrEMBL
Match: D7TFI5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_150.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00001087001 PE=4 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 3.672e-43
Identity = 89/117 (76.07%), Postives = 101/117 (86.32%), Query Frame = -1

Query:    6 LGLCRVDF*RFYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTN--TTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 350
            LG     F  F+ LFLE+LE V+QGKETSISH+ELV+RSRDQS+SDY+VMFFRFVT   I+ RSEFFEPFI+G+TN   TVEQFCKS+VEPMGEESDHVHIIALSDA+GVPIRVVYL
Sbjct:  135 LGYADFTFEDFFALFLEQLESVVQGKETSISHDELVIRSRDQSISDYIVMFFRFVTSGEIKRRSEFFEPFILGMTNATATVEQFCKSSVEPMGEESDHVHIIALSDALGVPIRVVYL 251

HSP 2 Score: 100.908 bits (250), Expect = 4.367e-20
Identity = 52/104 (50.00%), Postives = 66/104 (63.46%), Query Frame = -2

Query:  197 GHGRGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDFIRYSLRSWKM*FKGKRLP*VMKSLCL---EAEISLYLIMLLCF 499
            G  R TRGDGNCFFRGFMFSYLEH+LE+QD+AE+DR+  N+E+ R  LQ LGYA+ TFEDF    L   +   +GK        L +   +  IS Y++M   F
Sbjct:   85 GSIRRTRGDGNCFFRGFMFSYLEHILESQDQAEVDRVTTNVEKCRETLQALGYADFTFEDFFALFLEQLESVVQGKETSISHDELVIRSRDQSISDYIVMFFRF 188

[Close]

BLAST of GH720742 vs. TrEMBL
Match: B9GMT7_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_843967 PE=4 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 1.395e-42
Identity = 88/115 (76.52%), Postives = 98/115 (85.22%), Query Frame = -1

Query:    6 LGLCRVDF*RFYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 350
            LG     F  F+ LFLE+L+DV+QG E SISHEEL+ RSRDQSVSDYVVMFFRFVT   I+ RS+FFEPFI GLTNTTVEQFCKS+VEPMGEESDH+HI ALSDA+GVPIRVVYL
Sbjct:   80 LGYVDFTFEDFFALFLEQLDDVLQGNENSISHEELLNRSRDQSVSDYVVMFFRFVTTGEIRKRSQFFEPFIFGLTNTTVEQFCKSSVEPMGEESDHMHITALSDALGVPIRVVYL 194

HSP 2 Score: 95.5153 bits (236), Expect = 1.835e-18
Identity = 42/58 (72.41%), Postives = 49/58 (84.48%), Query Frame = -2

Query:  317 RGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDF 490
            R TRGDGNCFFR FMFSYLEH+LE QD+AE+DRIK+N+E  R  LQ+LGY + TFEDF
Sbjct:   33 RRTRGDGNCFFRSFMFSYLEHILETQDRAEVDRIKLNVEECRKTLQSLGYVDFTFEDF 90

[Close]

BLAST of GH720742 vs. TrEMBL
Match: B9T922_RICCO (Putative uncharacterized protein (Fragment) OS=Ricinus communis GN=RCOM_2004830 PE=4 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 5.302e-42
Identity = 87/115 (75.65%), Postives = 97/115 (84.35%), Query Frame = -1

Query:    6 LGLCRVDF*RFYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 350
            LG     F  F+ LFLE+L+ V+QG ETSISH+EL+ RSRDQS+SDYVVMFFRFVT   I+ RSEFFEPFI+GLTN TVEQFCKSAVEPMGEESDHVHI ALSDA+GV IRVVYL
Sbjct:  136 LGYVDFTFEDFFALFLEQLDSVLQGNETSISHDELINRSRDQSISDYVVMFFRFVTSGEIRKRSEFFEPFILGLTNATVEQFCKSAVEPMGEESDHVHITALSDALGVAIRVVYL 250

HSP 2 Score: 97.0561 bits (240), Expect = 6.307e-19
Identity = 43/58 (74.14%), Postives = 50/58 (86.21%), Query Frame = -2

Query:  317 RGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDF 490
            R TRGDGNCFFR FMFSYLEH+LE+QDK+E+DRIKVN+E  R  LQ+LGY + TFEDF
Sbjct:   89 RRTRGDGNCFFRSFMFSYLEHILESQDKSEVDRIKVNVEECRKTLQSLGYVDFTFEDF 146

[Close]

BLAST of GH720742 vs. TrEMBL
Match: D7KCS8_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_473019 PE=4 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 1.886e-39
Identity = 81/116 (69.83%), Postives = 98/116 (84.48%), Query Frame = -1

Query:    6 DLGLCRVDF*RFYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 353
            +LG     F  F+ LFLE+L+D++QG E SIS++ELV RSRDQSVSDY+VMFFRFVT   I+ R++FFEPFI GL+NTTV+QFCKS+VEPMGEESDH+HI ALSDA+GV IRVVYL
Sbjct:  132 NLGYTDFTFEDFFALFLEQLDDILQGTEESISYDELVNRSRDQSVSDYIVMFFRFVTAGDIRTRADFFEPFITGLSNTTVDQFCKSSVEPMGEESDHIHITALSDALGVAIRVVYL 247

HSP 2 Score: 97.4413 bits (241), Expect = 4.829e-19
Identity = 43/58 (74.14%), Postives = 50/58 (86.21%), Query Frame = -2

Query:  317 RGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDF 490
            R TRGDGNCFFR FMFSYLEH+LE+QD+AE+DRIKVN+E+ R  LQ LGY + TFEDF
Sbjct:   86 RRTRGDGNCFFRSFMFSYLEHILESQDRAEVDRIKVNVEKCRKTLQNLGYTDFTFEDF 143

[Close]

BLAST of GH720742 vs. TrEMBL
Match: Q6ZD38_ORYSJ (Putative ubiquitin-specific protease otubain 1 OS=Oryza sativa subsp. japonica GN=P0665C04.29 PE=4 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 2.463e-39
Identity = 81/117 (69.23%), Postives = 98/117 (83.76%), Query Frame = -1

Query:    6 SDLGLCRVDF*RFYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 356
            +DLG     F  F+++F+++LE V+QG E+SI  EEL+ R+RDQ VSDYVVMFFRFVT   IQ R+EFFEPFI GLTN+TV QFCK++VEPMGEESDHVHIIALSDA+GVPIRV+YL
Sbjct:   32 ADLGYIEFTFEDFFSIFIDQLESVLQGHESSIGAEELLERTRDQMVSDYVVMFFRFVTSGEIQRRAEFFEPFISGLTNSTVVQFCKASVEPMGEESDHVHIIALSDALGVPIRVMYL 148

HSP 2 Score: 59.6918 bits (143), Expect = 1.116e-7
Identity = 28/44 (63.64%), Postives = 33/44 (75.00%), Query Frame = -2

Query:  317 MFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDF 448
            MFSYLEH+LE QDKAE++RI   IE+ +  L  LGY E TFEDF
Sbjct:    1 MFSYLEHILETQDKAEVERILKKIEQCKKTLADLGYIEFTFEDF 44

[Close]

BLAST of GH720742 vs. TrEMBL
Match: Q0J434_ORYSJ (Os08g0537800 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os08g0537800 PE=4 SV=1)

HSP 1 Score: 164.851 bits (416), Expect = 2.463e-39
Identity = 81/117 (69.23%), Postives = 98/117 (83.76%), Query Frame = -1

Query:    6 SDLGLCRVDF*RFYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 356
            +DLG     F  F+++F+++LE V+QG E+SI  EEL+ R+RDQ VSDYVVMFFRFVT   IQ R+EFFEPFI GLTN+TV QFCK++VEPMGEESDHVHIIALSDA+GVPIRV+YL
Sbjct:   50 ADLGYIEFTFEDFFSIFIDQLESVLQGHESSIGAEELLERTRDQMVSDYVVMFFRFVTSGEIQRRAEFFEPFISGLTNSTVVQFCKASVEPMGEESDHVHIIALSDALGVPIRVMYL 166

HSP 2 Score: 85.1149 bits (209), Expect = 2.480e-15
Identity = 39/58 (67.24%), Postives = 45/58 (77.59%), Query Frame = -2

Query:  317 RGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDF 490
            R TRGDGNCF+R FMFSYLEH+LE QDKAE++RI   IE+ +  L  LGY E TFEDF
Sbjct:    5 RRTRGDGNCFYRSFMFSYLEHILETQDKAEVERILKKIEQCKKTLADLGYIEFTFEDF 62

[Close]

BLAST of GH720742 vs. SwissProt
Match: OTUBL_ARATH (Ubiquitin thioesterase otubain-like OS=Arabidopsis thaliana GN=At1g28120 PE=2 SV=2)

HSP 1 Score: 163.31 bits (412), Expect = 5.750e-40
Identity = 80/116 (68.97%), Postives = 97/116 (83.62%), Query Frame = -1

Query:    6 DLGLCRVDF*RFYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 353
            +LG     F  F+ LFLE+L+D++QG E SIS++ELV RSRDQSVSDY+VMFFRFVT   I+ R++FFEPFI GL+N TV+QFCKS+VEPMGEESDH+HI ALSDA+GV IRVVYL
Sbjct:  130 NLGYTDFTFEDFFALFLEQLDDILQGTEESISYDELVNRSRDQSVSDYIVMFFRFVTAGDIRTRADFFEPFITGLSNATVDQFCKSSVEPMGEESDHIHITALSDALGVAIRVVYL 245

HSP 2 Score: 97.4413 bits (241), Expect = 3.875e-20
Identity = 43/58 (74.14%), Postives = 50/58 (86.21%), Query Frame = -2

Query:  317 RGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDF 490
            R TRGDGNCFFR FMFSYLEH+LE+QD+AE+DRIKVN+E+ R  LQ LGY + TFEDF
Sbjct:   84 RRTRGDGNCFFRSFMFSYLEHILESQDRAEVDRIKVNVEKCRKTLQNLGYTDFTFEDF 141

[Close]

BLAST of GH720742 vs. SwissProt
Match: OTUB1_RAT (Ubiquitin thioesterase OTUB1 OS=Rattus norvegicus GN=Otub1 PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 8.036e-27
Identity = 47/105 (44.76%), Postives = 71/105 (67.62%), Query Frame = -1

Query:    6 FYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 320
            F+  F++ +E V   K+TS++  +L+    DQS SDY+V++ R +T   +Q  S+FFE FI G    TV++FC+  VEPM +ESDH+HIIAL+ A+ V I+V Y+
Sbjct:  138 FHNTFMDLIEQV--EKQTSVA--DLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEG--GRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYM 236

HSP 2 Score: 53.1434 bits (126), Expect = 8.036e-27
Identity = 28/58 (48.28%), Postives = 36/58 (62.07%), Query Frame = -2

Query:  317 RGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDF 490
            R TR DGNCF+R F FS+LE LL+  D  E+ R K    +S+  L + G+ E T EDF
Sbjct:   83 RKTRPDGNCFYRAFGFSHLEALLD--DSKELQRFKAVSAKSKEDLVSQGFTEFTIEDF 138

[Close]

BLAST of GH720742 vs. SwissProt
Match: OTUB1_MOUSE (Ubiquitin thioesterase OTUB1 OS=Mus musculus GN=Otub1 PE=1 SV=2)

HSP 1 Score: 87.4261 bits (215), Expect = 8.036e-27
Identity = 47/105 (44.76%), Postives = 71/105 (67.62%), Query Frame = -1

Query:    6 FYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 320
            F+  F++ +E V   K+TS++  +L+    DQS SDY+V++ R +T   +Q  S+FFE FI G    TV++FC+  VEPM +ESDH+HIIAL+ A+ V I+V Y+
Sbjct:  138 FHNTFMDLIEQV--EKQTSVA--DLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEG--GRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYM 236

HSP 2 Score: 53.1434 bits (126), Expect = 8.036e-27
Identity = 28/58 (48.28%), Postives = 36/58 (62.07%), Query Frame = -2

Query:  317 RGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDF 490
            R TR DGNCF+R F FS+LE LL+  D  E+ R K    +S+  L + G+ E T EDF
Sbjct:   83 RKTRPDGNCFYRAFGFSHLEALLD--DSKELQRFKAVSAKSKEDLVSQGFTEFTIEDF 138

[Close]

BLAST of GH720742 vs. SwissProt
Match: OTUB1_HUMAN (Ubiquitin thioesterase OTUB1 OS=Homo sapiens GN=OTUB1 PE=1 SV=2)

HSP 1 Score: 87.4261 bits (215), Expect = 8.036e-27
Identity = 47/105 (44.76%), Postives = 71/105 (67.62%), Query Frame = -1

Query:    6 FYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 320
            F+  F++ +E V   K+TS++  +L+    DQS SDY+V++ R +T   +Q  S+FFE FI G    TV++FC+  VEPM +ESDH+HIIAL+ A+ V I+V Y+
Sbjct:  138 FHNTFMDLIEQV--EKQTSVA--DLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEG--GRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYM 236

HSP 2 Score: 53.1434 bits (126), Expect = 8.036e-27
Identity = 28/58 (48.28%), Postives = 36/58 (62.07%), Query Frame = -2

Query:  317 RGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDF 490
            R TR DGNCF+R F FS+LE LL+  D  E+ R K    +S+  L + G+ E T EDF
Sbjct:   83 RKTRPDGNCFYRAFGFSHLEALLD--DSKELQRFKAVSAKSKEDLVSQGFTEFTIEDF 138

[Close]

BLAST of GH720742 vs. SwissProt
Match: OTUBL_DROME (Ubiquitin thioesterase otubain-like OS=Drosophila melanogaster GN=CG4968 PE=1 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.485e-21
Identity = 42/107 (39.25%), Postives = 62/107 (57.94%), Query Frame = -1

Query:    6 FYTLFLEKLEDVIQGKETSIS--HEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 320
            F+  F+E ++ V        S   +EL     +Q  SDYVV++ R +T   +Q  ++F++ FI G  + T+E F    VEPM +ESDH+HIIAL  A+G  +RV YL
Sbjct:  122 FHETFMEVIQRVSPDNAGGHSTVQDELHKIFNEQGYSDYVVVYLRLITSGKLQEEADFYQNFIEG--DLTIEAFRHLEVEPMYKESDHIHIIALCTALGAGVRVEYL 226

HSP 2 Score: 49.6766 bits (117), Expect = 2.485e-21
Identity = 26/58 (44.83%), Postives = 33/58 (56.90%), Query Frame = -2

Query:  317 RGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDF 490
            R TR DGNCFFR F +SYLE+L+   + +     K   E S+  L  LG+   T EDF
Sbjct:   67 RRTRPDGNCFFRAFAYSYLEYLI--SNTSAYQEFKKLAEESKEKLVQLGFPSFTLEDF 122

[Close]

BLAST of GH720742 vs. SwissProt
Match: OTUB2_HUMAN (Ubiquitin thioesterase OTUB2 OS=Homo sapiens GN=OTUB2 PE=1 SV=2)

HSP 1 Score: 68.1662 bits (165), Expect = 1.452e-17
Identity = 37/101 (36.63%), Postives = 54/101 (53.47%), Query Frame = -1

Query:    6 FLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 308
            F      V++  E   S   L+    DQS SD++V F R +T   I+NR++FF  FI       ++ FC   VEPM  E DH+ I ALS A+ + ++V Y+
Sbjct:   98 FFNAFYSVVELVEKDGSVSSLLKVFNDQSASDHIVQFLRLLTSAFIRNRADFFRHFID--EEMDIKDFCTHEVEPMATECDHIQITALSQALSIALQVEYV 196

HSP 2 Score: 41.2022 bits (95), Expect = 1.452e-17
Identity = 23/58 (39.66%), Postives = 32/58 (55.17%), Query Frame = -2

Query:  317 RGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDF 490
            R T+GDGNCF+R   +SYLE LL      EI + K  + ++   L   G+ E  F +F
Sbjct:   43 RKTKGDGNCFYRALGYSYLESLL--GKSREIFKFKERVLQTPNDLLAAGFEEHKFRNF 98

[Close]

BLAST of GH720742 vs. SwissProt
Match: OTUB2_MOUSE (Ubiquitin thioesterase OTUB2 OS=Mus musculus GN=Otub2 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 4.148e-17
Identity = 42/108 (38.89%), Postives = 60/108 (55.56%), Query Frame = -1

Query:    6 F*RFYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 329
            F  F+  F   +E V   K++S+S   L+    DQS SD +V F R +T   I+NR++FF  FI       ++ FC   VEPM  E DHV I ALS A+ + ++V Y+
Sbjct:   95 FRNFFNAFYSVVELV--EKDSSVS--SLLKVFNDQSSSDRIVQFLRLLTSAFIRNRADFFRHFID--EEMDIKDFCTHEVEPMAMECDHVQITALSQALNIALQVEYV 196

HSP 2 Score: 40.817 bits (94), Expect = 4.148e-17
Identity = 23/58 (39.66%), Postives = 32/58 (55.17%), Query Frame = -2

Query:  317 RGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDF 490
            R T+GDGNCF+R   +SYLE LL      EI + K  + ++   L   G+ E  F +F
Sbjct:   43 RKTKGDGNCFYRALGYSYLESLL--GKSREILKFKERVLQTPNDLLAAGFEEHKFRNF 98

[Close]

BLAST of GH720742 vs. SwissProt
Match: OTUBL_CAEBR (Ubiquitin thioesterase otubain-like OS=Caenorhabditis briggsae GN=otub-1 PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 2.733e-15
Identity = 36/109 (33.03%), Postives = 64/109 (58.72%), Query Frame = -1

Query:    6 DF*RFYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 332
            DF  F+  F+EK+ D I  KE      ++     D + ++Y++MFFR +T   ++  +  +EPF+      ++ Q+C++ +E M +ESDH+ IIAL  A+ + IR+ Y+
Sbjct:  133 DFCEFFIEFIEKVRDGIHQKE------DVFRIFNDDNTANYLLMFFRLITSGYLKEHAAEYEPFLD--EGMSLAQYCETEIEAMWKESDHLGIIALVRALNIRIRIEYM 233

HSP 2 Score: 35.039 bits (79), Expect = 2.733e-15
Identity = 21/63 (33.33%), Postives = 29/63 (46.03%), Query Frame = -2

Query:  308 HGRGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDFIRY 496
            H R  RGDGNCF R      +E LL   DK  + +   + +     L  LG+ + T  DF  +
Sbjct:   77 HIRFIRGDGNCFIRAIQVGLVEILL--NDKERLVKFIASCKEWTERLVKLGFPDWTCTDFCEF 137

[Close]

BLAST of GH720742 vs. SwissProt
Match: OTUBL_CAEEL (Ubiquitin thioesterase otubain-like OS=Caenorhabditis elegans GN=otub-1 PE=1 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 2.734e-15
Identity = 35/109 (32.11%), Postives = 59/109 (54.13%), Query Frame = -1

Query:    6 DF*RFYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 332
            DF  F+  FLEK+   +       + E +     D   ++Y++MFFR +T   ++  SE + PFI      TV Q+C+  +EPM +++DH+ I +L  A G  +R+ Y+
Sbjct:  134 DFCDFFIEFLEKIHSGVH------TEEAVYTILNDDGSANYILMFFRLITSAFLKQNSEEYAPFID--EGMTVAQYCEQEIEPMWKDADHLAINSLIKAAGTRVRIEYM 234

HSP 2 Score: 36.965 bits (84), Expect = 2.734e-15
Identity = 20/61 (32.79%), Postives = 32/61 (52.46%), Query Frame = -2

Query:  317 GHGRGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDF 499
            G  R  RGDGNCF+R  +   +E +L  +D+A +++   +       L  LG+ + T  DF
Sbjct:   77 GEIRYIRGDGNCFYRAILVGLIEIML--KDRARLEKFIASSRDWTRTLVELGFPDWTCTDF 135

[Close]

BLAST of GH720742 vs. TAIR peptide
Match: AT1G28120.1 (| Symbols: | CONTAINS InterPro DOMAIN/s: Ovarian tumour, otubain (InterPro:IPR003323), Ubiquitin thioesterase Otubain (InterPro:IPR016615), Peptidase C65, otubain (InterPro:IPR019400); Has 413 Blast hits to 411 proteins in 139 species: Archae - 0; Bacteria - 0; Metazoa - 184; Fungi - 83; Plants - 88; Viruses - 0; Other Eukaryotes - 58 (source: NCBI BLink). | chr1:9813219-9815143 REVERSE LENGTH=306)

HSP 1 Score: 163.31 bits (412), Expect = 6.347e-41
Identity = 80/116 (68.97%), Postives = 97/116 (83.62%), Query Frame = -1

Query:    6 DLGLCRVDF*RFYTLFLEKLEDVIQGKETSISHEELVLRSRDQSVSDYVVMFFRFVTYVAIQNRSEFFEPFIMGLTNTTVEQFCKSAVEPMGEESDHVHIIALSDAMGVPIRVVYL 353
            +LG     F  F+ LFLE+L+D++QG E SIS++ELV RSRDQSVSDY+VMFFRFVT   I+ R++FFEPFI GL+N TV+QFCKS+VEPMGEESDH+HI ALSDA+GV IRVVYL
Sbjct:  130 NLGYTDFTFEDFFALFLEQLDDILQGTEESISYDELVNRSRDQSVSDYIVMFFRFVTAGDIRTRADFFEPFITGLSNATVDQFCKSSVEPMGEESDHIHITALSDALGVAIRVVYL 245

HSP 2 Score: 97.4413 bits (241), Expect = 4.277e-21
Identity = 43/58 (74.14%), Postives = 50/58 (86.21%), Query Frame = -2

Query:  317 RGTRGDGNCFFRGFMFSYLEHLLEAQDKAEIDRIKVNIERSR*ALQTLGYAELTFEDF 490
            R TRGDGNCFFR FMFSYLEH+LE+QD+AE+DRIKVN+E+ R  LQ LGY + TFEDF
Sbjct:   84 RRTRGDGNCFFRSFMFSYLEHILESQDRAEVDRIKVNVEKCRKTLQNLGYTDFTFEDF 141

The following BLAST results are available for this feature:

BLAST of GH720742 vs. TrEMBL
BLAST of GH720742 vs. SwissProt
BLAST of GH720742 vs. TAIR peptide

BLAST of GH720742 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FHP1_MEDTR	3.791e-48	85.22	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6TEY7_SOYBN	7.149e-47	81.74	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9GZ22_POPTR	9.662e-44	78.26	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A7YFC1_POPTR	9.662e-44	78.26	Otubain-like cysteine protease (Fragment) OS=Popul...	[more]
D7TFI5_VITVI	3.672e-43	76.07	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9GMT7_POPTR	1.395e-42	76.52	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
B9T922_RICCO	5.302e-42	75.65	Putative uncharacterized protein (Fragment) OS=Ric...	[more]
D7KCS8_ARALY	1.886e-39	69.83	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q6ZD38_ORYSJ	2.463e-39	69.23	Putative ubiquitin-specific protease otubain 1 OS=...	[more]
Q0J434_ORYSJ	2.463e-39	69.23	Os08g0537800 protein (Fragment) OS=Oryza sativa su...	[more]

BLAST of GH720742 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 9


Match Name	E-value	Identity	Description
OTUBL_ARATH	5.750e-40	68.97	Ubiquitin thioesterase otubain-like OS=Arabidopsis...	[more]
OTUB1_RAT	8.036e-27	44.76	Ubiquitin thioesterase OTUB1 OS=Rattus norvegicus ...	[more]
OTUB1_MOUSE	8.036e-27	44.76	Ubiquitin thioesterase OTUB1 OS=Mus musculus GN=Ot...	[more]
OTUB1_HUMAN	8.036e-27	44.76	Ubiquitin thioesterase OTUB1 OS=Homo sapiens GN=OT...	[more]
OTUBL_DROME	2.485e-21	39.25	Ubiquitin thioesterase otubain-like OS=Drosophila ...	[more]
OTUB2_HUMAN	1.452e-17	36.63	Ubiquitin thioesterase OTUB2 OS=Homo sapiens GN=OT...	[more]
OTUB2_MOUSE	4.148e-17	38.89	Ubiquitin thioesterase OTUB2 OS=Mus musculus GN=Ot...	[more]
OTUBL_CAEBR	2.733e-15	33.03	Ubiquitin thioesterase otubain-like OS=Caenorhabdi...	[more]
OTUBL_CAEEL	2.734e-15	32.11	Ubiquitin thioesterase otubain-like OS=Caenorhabdi...	[more]

BLAST of GH720742 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G28120.1	6.347e-41	68.97	\| Symbols: \| CONTAINS InterPro DOMAIN/s: Ovarian ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019400	Peptidase C65, otubain	PFAM	PF10275	Peptidase_C65	coord: 2..106 score: 8
None	No IPR available	PANTHER	PTHR12931	UBIQUITIN THIOLESTERASE PROTEIN OTUB	coord: 2..106 score: 2.3
None	No IPR available	PANTHER	PTHR12931:SF6	UBIQUITIN THIOLESTERASE PROTEIN OTUB1	coord: 2..106 score: 2.3
None	No IPR available	SUPERFAMILY	54001	Cysteine proteinases	coord: 2..106 score: 9.3

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720742 ID=GH720742; Name=GH720742; organism=Pisum sativum; type=EST; length=503bp
CCGGGCAGGTACACAACACGGATGGGAACGCCCATTGCATCTGAAAGGGC
AATAATGTGCACATGGTCGCTCTCTTCGCCCATTGGTTCAACAGCTGATT
TGCAAAACTGCTCAACTGTTGTATTGGTTAAGCCCATTATGAAAGGTTCA
AAAAATTCTGAGCGGTTTTGTATAGCAACATACGTGACAAATCTGAAAAA
CATAACAACATAATCAGATACAGACTGATCTCGGCTTCTAAGCACAAGCT
CTTCATGACTTATGGAAGTCTCTTTCCCTTGAATTACATCTTCCAGCTTC
TCAAGGAATAGCGTATAAAATCTTCAAAAGTCAACTCTGCATAACCCAAG
GTCTGAAGTGCTTATCTACTTCTCTCAATATTAACCTTGATGCGATCAAT
TTCTGCTTTGTCTTGAGCTTCCAAAAGATGCTCGAGGTATGAAAACATAA
AGCCTCGAAAGAAGCAGTTTCCATCTCCCCGAGTACCTCGGCCGTGACCA
CGC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR019400	Peptidase_C65_otubain

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