GH720834

EST Overview


Name	GH720834
Unique Name	GH720834
Type	EST
Organism	Pisum sativum (pea)
Sequence length	565

Libraries


Library Name	Type
LIBEST_024273 BACNI	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of GH720834 vs. TrEMBL
Match: D7KIB3_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_472261 PE=4 SV=1)

HSP 1 Score: 268.47 bits (685), Expect = 2.241e-70
Identity = 123/144 (85.42%), Postives = 138/144 (95.83%), Query Frame = -1

Query:  116 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWWSRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGE 547
            YSIEATCHAPKLEEVYAEI+RVLKPG++YVSYEWVTTEKF +E+ EHVE+IQGIERGDALPGLR+Y DIAE AKKVGFE+VKE+DLA PPA+PWW+RLKMGRLAYWRNH+VV +LSA+G+APKGTVDVHEMLFKTADYLTRGGE
Sbjct:  196 YSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDDEHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAKPWWTRLKMGRLAYWRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGE 339

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BLAST of GH720834 vs. TrEMBL
Match: D2D5G3_SOYBN (Sterol 24-C methyltransferase 2-1 OS=Glycine max GN=SMT2-1 PE=2 SV=1)

HSP 1 Score: 268.47 bits (685), Expect = 2.241e-70
Identity = 125/144 (86.81%), Postives = 138/144 (95.83%), Query Frame = -1

Query:  116 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWWSRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGE 547
            YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTT+K+  ++ EHVE+IQGIERGDALPGLR+Y DIAE A+KVGF VVKERDLA+PPA+PWWSRLKMGR+AYWRNH+VVTVL+ALGIAPKGTVDVHEMLFKTADYLTRGG+
Sbjct:  196 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTDKYRGDDPEHVEVIQGIERGDALPGLRNYTDIAETARKVGFAVVKERDLAKPPAQPWWSRLKMGRIAYWRNHIVVTVLAALGIAPKGTVDVHEMLFKTADYLTRGGD 339

[Close]

BLAST of GH720834 vs. TrEMBL
Match: D2D5G4_SOYBN (Sterol 24-C methyltransferase 2-2 OS=Glycine max GN=SMT2-2 PE=2 SV=1)

HSP 1 Score: 268.085 bits (684), Expect = 2.927e-70
Identity = 126/144 (87.50%), Postives = 138/144 (95.83%), Query Frame = -1

Query:  116 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWWSRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGE 547
            YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTT+K+  ++ EHVE+IQGIERGDALPGLRSYA+IAE A+KVGF VVKERDLA+PPA PWWSRLKMGR+AYWRNH+VVTVL+ALGIAPKGTVDVHEMLFKTADYLTRGG+
Sbjct:  196 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTDKYSGDDPEHVEVIQGIERGDALPGLRSYAEIAETARKVGFAVVKERDLAKPPALPWWSRLKMGRIAYWRNHIVVTVLAALGIAPKGTVDVHEMLFKTADYLTRGGD 339

[Close]

BLAST of GH720834 vs. TrEMBL
Match: B9R8F7_RICCO (S-adenosyl-l-methionine:delta24-sterol-C-methyltransferase, putative OS=Ricinus communis GN=RCOM_1599550 PE=4 SV=1)

HSP 1 Score: 268.085 bits (684), Expect = 2.927e-70
Identity = 124/144 (86.11%), Postives = 140/144 (97.22%), Query Frame = -1

Query:  116 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWWSRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGE 547
            YSIEATCHAPKLEEVYAEIFRVLKPG+LYVSYEWVTT+K+ + +SEHVEIIQGIERGDALPGLR+Y DIAE A+KVGFEVVKE+DLA+PPA+PWW+RLKMGR+AYWRNH++VT+LSA+GIAPKGTVDVHEMLFKTADYLTRGGE
Sbjct:  196 YSIEATCHAPKLEEVYAEIFRVLKPGSLYVSYEWVTTDKYRASDSEHVEIIQGIERGDALPGLRNYIDIAETARKVGFEVVKEKDLAKPPAQPWWTRLKMGRIAYWRNHILVTMLSAIGIAPKGTVDVHEMLFKTADYLTRGGE 339

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BLAST of GH720834 vs. TrEMBL
Match: A9P9Q4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_559888 PE=2 SV=1)

HSP 1 Score: 266.544 bits (680), Expect = 8.516e-70
Identity = 122/144 (84.72%), Postives = 140/144 (97.22%), Query Frame = -1

Query:  116 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWWSRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGE 547
            YSIEATCHAPKLEEVYAEIFRVLKPG+LYVSYEWVTT+K+ S + EHVE+IQGIERGDALPGLR+Y+DIAE A+KVGFEVVKE+DLA+PPA+PWW+RL+MGR+AYWRNH+VVT+LSALGIAPKGTVDVHEMLFKTADYLT+GG+
Sbjct:  196 YSIEATCHAPKLEEVYAEIFRVLKPGSLYVSYEWVTTDKYKSSDPEHVEVIQGIERGDALPGLRNYSDIAETARKVGFEVVKEKDLAKPPAQPWWTRLQMGRIAYWRNHIVVTILSALGIAPKGTVDVHEMLFKTADYLTKGGD 339

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BLAST of GH720834 vs. TrEMBL
Match: A9PE86_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_815989 PE=2 SV=1)

HSP 1 Score: 265.388 bits (677), Expect = 1.897e-69
Identity = 122/144 (84.72%), Postives = 138/144 (95.83%), Query Frame = -1

Query:  116 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWWSRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGE 547
            YSIEATCHAPKLEEVYAEIFRVLKPG+LYVSYEWVTT+K+   + EHVE+IQGIERGDALPGLR+Y DIAE A+KVGFEVVKE+DLA+PPA+PWW+RLKMGR+AYWRNH+VVTVLSA+GIAPKGTVDVHEMLFKTADYLT+GG+
Sbjct:  196 YSIEATCHAPKLEEVYAEIFRVLKPGSLYVSYEWVTTDKYKDSDPEHVEVIQGIERGDALPGLRNYTDIAETARKVGFEVVKEKDLAKPPAQPWWTRLKMGRIAYWRNHIVVTVLSAVGIAPKGTVDVHEMLFKTADYLTKGGD 339

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BLAST of GH720834 vs. TrEMBL
Match: B0FDL8_GOSHI (24-sterol C-methyltransferase OS=Gossypium hirsutum GN=SMT2-2 PE=2 SV=1)

HSP 1 Score: 265.003 bits (676), Expect = 2.478e-69
Identity = 120/144 (83.33%), Postives = 139/144 (96.53%), Query Frame = -1

Query:  116 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWWSRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGE 547
            YSIEATCHAPKLEEVYAE+FRVLKPG+LYVSYEWVTT+++  +N EHVE+IQGIERGDALPGLR+Y DIAE+AKKVGFEVVKE+DLA+PPA PWW+RLKMGR+AYWRNH++VTVL+++GIAPKGTVDVHEMLFKTADYLTRGG+
Sbjct:  196 YSIEATCHAPKLEEVYAEVFRVLKPGSLYVSYEWVTTDRYRGDNPEHVEVIQGIERGDALPGLRNYKDIAEVAKKVGFEVVKEKDLAKPPALPWWTRLKMGRIAYWRNHILVTVLASIGIAPKGTVDVHEMLFKTADYLTRGGD 339

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BLAST of GH720834 vs. TrEMBL
Match: B0FDL7_GOSHI (24-sterol C-methyltransferase OS=Gossypium hirsutum GN=SMT2-1 PE=2 SV=1)

HSP 1 Score: 263.462 bits (672), Expect = 7.209e-69
Identity = 120/144 (83.33%), Postives = 139/144 (96.53%), Query Frame = -1

Query:  116 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWWSRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGE 547
            YSIEATCHAPKLE+VYAE+FRVLKPG+LYVSYEWVTTEK+ ++N EHVE+IQGIERGDALPGLRSY+DIAE AKKVGFEVVKE+DLA+PP+ PWW+RLKMGR+AYWRNH++V VL+A+GIAPKGTVDVH+MLFKTADYLTRGG+
Sbjct:  196 YSIEATCHAPKLEDVYAEVFRVLKPGSLYVSYEWVTTEKYRADNPEHVEVIQGIERGDALPGLRSYSDIAEAAKKVGFEVVKEKDLAKPPSLPWWTRLKMGRIAYWRNHILVIVLAAIGIAPKGTVDVHDMLFKTADYLTRGGD 339

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BLAST of GH720834 vs. TrEMBL
Match: D7KT96_ARALY (S-adenosyl-methionine-sterol-C-methyltransferase 3 OS=Arabidopsis lyrata subsp. lyrata GN=SMT3 PE=4 SV=1)

HSP 1 Score: 259.61 bits (662), Expect = 1.041e-67
Identity = 119/144 (82.64%), Postives = 136/144 (94.44%), Query Frame = -1

Query:  116 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWWSRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGE 547
            YSIEATCHAPKLEEVY+EIFRV+KPG+L+VSYEWVTTEK+  E+ EH ++IQGIERGDALPGLRSY DIA  AKKVGFEVVKE+DLA+PP++PWW+RLKMGRLAYWRNHVVV +LSA+G+APKGTVDVH+MLFKTADYLTRGGE
Sbjct:  196 YSIEATCHAPKLEEVYSEIFRVMKPGSLFVSYEWVTTEKYRDEDEEHKDVIQGIERGDALPGLRSYIDIAATAKKVGFEVVKEKDLAKPPSKPWWNRLKMGRLAYWRNHVVVVILSAIGVAPKGTVDVHKMLFKTADYLTRGGE 339

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BLAST of GH720834 vs. TrEMBL
Match: A5BH10_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_041951 PE=4 SV=1)

HSP 1 Score: 255.373 bits (651), Expect = 1.963e-66
Identity = 116/144 (80.56%), Postives = 135/144 (93.75%), Query Frame = -1

Query:  116 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWWSRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGE 547
            YSIEATCHAP+LEEVYAEIFRVLKPG++YVSYEWVTT+KF +E+ EHV++IQGIERGDALPGLR Y+DIA  A+KVGFEVV E+DLA+PPA PWW+RLKMGR+AYWRNH+++ + +ALGIAPKGTVDVHEMLFKTADYLTRGGE
Sbjct:  196 YSIEATCHAPELEEVYAEIFRVLKPGSMYVSYEWVTTDKFKAEDPEHVDVIQGIERGDALPGLRPYSDIAATARKVGFEVVXEKDLAKPPAMPWWTRLKMGRIAYWRNHILIMIFAALGIAPKGTVDVHEMLFKTADYLTRGGE 339

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BLAST of GH720834 vs. SwissProt
Match: SMT2_ARATH (24-methylenesterol C-methyltransferase 2 OS=Arabidopsis thaliana GN=SMT2 PE=1 SV=2)

HSP 1 Score: 270.011 bits (689), Expect = 5.720e-72
Identity = 124/144 (86.11%), Postives = 138/144 (95.83%), Query Frame = -1

Query:  116 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWWSRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGE 547
            YSIEATCHAPKLEEVYAEI+RVLKPG++YVSYEWVTTEKF +E+ EHVE+IQGIERGDALPGLR+Y DIAE AKKVGFE+VKE+DLA PPAEPWW+RLKMGRLAYWRNH+VV +LSA+G+APKGTVDVHEMLFKTADYLTRGGE
Sbjct:  196 YSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDDEHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAYWRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGE 339

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BLAST of GH720834 vs. SwissProt
Match: SMT3B_ARATH (24-methylenesterol C-methyltransferase 3 OS=Arabidopsis thaliana GN=SMT3 PE=2 SV=1)

HSP 1 Score: 259.225 bits (661), Expect = 1.010e-68
Identity = 118/144 (81.94%), Postives = 137/144 (95.14%), Query Frame = -1

Query:  116 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWWSRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGE 547
            YSIEATCHAPKLEEVY+EIFRV+KPG+L+VSYEWVTTEK+  ++ EH ++IQGIERGDALPGLRSYADIA  AKKVGFEVVKE+DLA+PP++PWW+RLKMGR+AYWRNHVVV +LSA+G+APKGTVDVH+MLFKTADYLTRGGE
Sbjct:  196 YSIEATCHAPKLEEVYSEIFRVMKPGSLFVSYEWVTTEKYRDDDEEHKDVIQGIERGDALPGLRSYADIAVTAKKVGFEVVKEKDLAKPPSKPWWNRLKMGRIAYWRNHVVVVILSAIGVAPKGTVDVHKMLFKTADYLTRGGE 339

[Close]

BLAST of GH720834 vs. SwissProt
Match: SMT2_ORYSJ (24-methylenesterol C-methyltransferase 2 OS=Oryza sativa subsp. japonica GN=Smt2-1 PE=2 SV=2)

HSP 1 Score: 221.861 bits (564), Expect = 1.786e-57
Identity = 104/144 (72.22%), Postives = 122/144 (84.72%), Query Frame = -1

Query:  116 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWWSRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGE 547
            YSIEATCHAP+L++VY E+FRVLKPG LYVSYEWVTT  + ++N EHVE I GIERGDALPGLR   +IA IAK+VGFEV+KE DLA PPA PWW+RLKMGR+AYWRN +VV VL+ L IAPKG  +VHEML++TA +LTRGGE
Sbjct:  201 YSIEATCHAPRLQDVYGEVFRVLKPGGLYVSYEWVTTSLYRADNPEHVEAIHGIERGDALPGLRRQDEIASIAKEVGFEVLKELDLALPPALPWWTRLKMGRIAYWRNSLVVRVLTMLRIAPKGVCEVHEMLYETAQHLTRGGE 344

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BLAST of GH720834 vs. SwissProt
Match: ERG6_KLULA (Sterol 24-C-methyltransferase OS=Kluyveromyces lactis GN=ERG6 PE=3 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 8.093e-26
Identity = 68/164 (41.46%), Postives = 88/164 (53.66%), Query Frame = -1

Query:  110 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAE-PWWSRL-----------------KMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGEHK 547
            Y+IEATCHAP  E VY EI++VLKPG  +  YEWV T+K+   N EH +I   IE GD +P + S     +   KVGFE+++E DLA    E PW++ L                 +  RL       +VTV   LG+APKG+V V   L + A  L  GGE K
Sbjct:  192 YAIEATCHAPTFEGVYGEIYKVLKPGGTFAVYEWVMTDKYDETNPEHRKIAYEIELGDGIPKMYSVDAARDALSKVGFEILEENDLADNDDEIPWYAPLTGEWKYVNSVADLATFFRTSRLGRAFTTSMVTVFEKLGLAPKGSVSVTNALEEAAVGLVAGGEKK 355

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BLAST of GH720834 vs. SwissProt
Match: ERG6_PNECA (Sterol 24-C-methyltransferase OS=Pneumocystis carinii GN=erg6 PE=2 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 2.604e-24
Identity = 62/166 (37.35%), Postives = 90/166 (54.22%), Query Frame = -1

Query:  104 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAE-PWW-----------------SRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGEHKKE 547
            YSIEAT HAP LE VY+EI+RVLKPG LY SYEWV   K+   + EH +I+ GIE GD++P +    +      KVGFE++   +L+   +  PW+                 S  +M  +  W     + ++  +G+ PKG+  V+++L   AD L + G  KKE
Sbjct:  199 YSIEATIHAPSLEGVYSEIYRVLKPGGLYASYEWVMLNKYDENDPEHQQIVYGIEIGDSIPKISKIGEAEAALIKVGFEIIHSEELSTKNSPLPWYYYLDGDLRKVRSFRDFISIARMTTIGKWLISSFIGLMEFIGLLPKGSKKVNDILLVAADSLVKAG--KKE 362

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BLAST of GH720834 vs. SwissProt
Match: ERG6_YEAST (Sterol 24-C-methyltransferase OS=Saccharomyces cerevisiae GN=ERG6 PE=1 SV=4)

HSP 1 Score: 108.997 bits (271), Expect = 1.688e-23
Identity = 69/166 (41.57%), Postives = 91/166 (54.82%), Query Frame = -1

Query:  110 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIA-KKVGFEVVKERDLAQPPAE-PWWSRLKMGRLAYWRN------------------HVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGEHK 547
            Y+IEATCHAPKLE VY+EI++VLKPG  +  YEWV T+K+   N EH +I   IE GD +P +  + D+A  A K  GFEV+   DLA    E PW+  L  G   Y +N                    +VTV+  LG+AP+G+ +V   L   A  L  GG+ K
Sbjct:  192 YAIEATCHAPKLEGVYSEIYKVLKPGGTFAVYEWVMTDKYDENNPEHRKIAYEIELGDGIPKM-FHVDVARKALKNCGFEVLVSEDLADNDDEIPWYYPL-TGEWKYVQNLANLATFFRTSYLGRQFTTAMVTVMEKLGLAPEGSKEVTAALENAAVGLVAGGKSK 355

[Close]

BLAST of GH720834 vs. SwissProt
Match: ERG6_CANGA (Sterol 24-C-methyltransferase OS=Candida glabrata GN=ERG6 PE=3 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 1.688e-23
Identity = 64/164 (39.02%), Postives = 88/164 (53.66%), Query Frame = -1

Query:  110 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAE-PWWSRL--------------KMGRLAYWR---NHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGEHK 547
            Y+IEATCHAPKLE VY EI++VLKPG  +  YEWV TE +   N EH +I   IE GD +P + +     +  K VGFEV   +DLA    E PW+  L                 R +Y+     + +V+++  +G+AP+G+V+V   L   A  L  GG  K
Sbjct:  192 YAIEATCHAPKLEGVYGEIYKVLKPGGTFAVYEWVMTENYDENNEEHRKIAYEIELGDGIPKMFTVDVAKQALKNVGFEVEVSKDLADEDDEIPWYYPLTGEWKYVQSLSDIATFFRTSYFGRKFTNCMVSIMEKVGVAPQGSVEVTAALENAAVGLVEGGRKK 355

[Close]

BLAST of GH720834 vs. SwissProt
Match: SMT1_ORYSJ (Cycloartenol-C-24-methyltransferase 1 OS=Oryza sativa subsp. japonica GN=Smt1-1 PE=2 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 4.910e-23
Identity = 63/167 (37.72%), Postives = 90/167 (53.89%), Query Frame = -1

Query:   77 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWWSRLKMGR--LAYWR--------NHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGEHKKED*WEPIHHF 547
            Y+IEATCHAP     Y EI+RVLKPG  +  YEW  T+ +   N+ H  I   IE G+ LP +RS     + AK  GFEV+ ++DLA+    PW+  L   R  L+ +R           +V  L  +G+AP+G+  V   L K A+ L  GG   K++ + P++ F
Sbjct:  173 YAIEATCHAPDPVGCYKEIYRVLKPGQCFAVYEWCITDHYEPNNATHKRIKDEIELGNGLPDIRSTQQCLQAAKDAGFEVIWDKDLAEDSPVPWYLPLDPSRFSLSSFRLTTVGRAITRTMVKALEYVGLAPQGSERVSNFLEKAAEGLVEGG---KKEIFTPMYFF 336

[Close]

BLAST of GH720834 vs. SwissProt
Match: ERG6_YARLI (Sterol 24-C-methyltransferase OS=Yarrowia lipolytica GN=ERG6 PE=3 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 4.910e-23
Identity = 65/164 (39.63%), Postives = 90/164 (54.88%), Query Frame = -1

Query:  110 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAE-PWWSRL--------------KMGRLAYWRNHVVVTVLSAL---GIAPKGTVDVHEMLFKTADYLTRGGEHK 547
            Y+IEAT HAP LE VY+EIFRVLKPG ++  YEWV T+++   N EH +I  GIE+GD +P +       +  + VGF++  + DLA   AE PW+  L               +GR +     V + V+ AL   G+AP+G+  V E L   A  L  GG+ K
Sbjct:  201 YAIEATVHAPVLEGVYSEIFRVLKPGGVFGVYEWVMTDEYDESNPEHRDICYGIEKGDGIPKMYKREVAVKALENVGFDIEYQEDLAADDAEVPWYYPLAGEWKYVQSLNDIVTIGRTSRLGRMVTMNVIGALEKIGLAPQGSRQVTEALEDAAVNLVAGGKKK 364

[Close]

BLAST of GH720834 vs. SwissProt
Match: SMT1_ARATH (Cycloartenol-C-24-methyltransferase OS=Arabidopsis thaliana GN=SMT1 PE=2 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 8.375e-23
Identity = 58/156 (37.18%), Postives = 85/156 (54.49%), Query Frame = -1

Query:  110 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWW----------SRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGEHK 547
            Y+IEATCHAP     Y EI+RVLKPG  + +YEW  T+ F  +N+EH +I   IE GD LP +R      E  K+ GFEV+ E+DLA+    PW+          S  ++  +  +    +V +L  + +AP+G+  V   L + A+ L  GG  +
Sbjct:  167 YAIEATCHAPDAYGCYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRRE 322

[Close]

BLAST of GH720834 vs. TAIR peptide
Match: AT1G20330.1 (| Symbols: SMT2, CVP1, FRL1 | sterol methyltransferase 2 | chr1:7038968-7040053 REVERSE LENGTH=361)

HSP 1 Score: 270.011 bits (689), Expect = 6.097e-73
Identity = 124/144 (86.11%), Postives = 138/144 (95.83%), Query Frame = -1

Query:  116 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWWSRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGE 547
            YSIEATCHAPKLEEVYAEI+RVLKPG++YVSYEWVTTEKF +E+ EHVE+IQGIERGDALPGLR+Y DIAE AKKVGFE+VKE+DLA PPAEPWW+RLKMGRLAYWRNH+VV +LSA+G+APKGTVDVHEMLFKTADYLTRGGE
Sbjct:  196 YSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDDEHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAYWRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGE 339

[Close]

BLAST of GH720834 vs. TAIR peptide
Match: AT1G76090.1 (| Symbols: SMT3 | sterol methyltransferase 3 | chr1:28550592-28551671 REVERSE LENGTH=359)

HSP 1 Score: 259.225 bits (661), Expect = 1.076e-69
Identity = 118/144 (81.94%), Postives = 137/144 (95.14%), Query Frame = -1

Query:  116 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWWSRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGE 547
            YSIEATCHAPKLEEVY+EIFRV+KPG+L+VSYEWVTTEK+  ++ EH ++IQGIERGDALPGLRSYADIA  AKKVGFEVVKE+DLA+PP++PWW+RLKMGR+AYWRNHVVV +LSA+G+APKGTVDVH+MLFKTADYLTRGGE
Sbjct:  196 YSIEATCHAPKLEEVYSEIFRVMKPGSLFVSYEWVTTEKYRDDDEEHKDVIQGIERGDALPGLRSYADIAVTAKKVGFEVVKEKDLAKPPSKPWWNRLKMGRIAYWRNHVVVVILSAIGVAPKGTVDVHKMLFKTADYLTRGGE 339

[Close]

BLAST of GH720834 vs. TAIR peptide
Match: AT5G13710.2 (| Symbols: SMT1, CPH | sterol methyltransferase 1 | chr5:4424048-4426866 REVERSE LENGTH=336)

HSP 1 Score: 106.686 bits (265), Expect = 8.927e-24
Identity = 58/156 (37.18%), Postives = 85/156 (54.49%), Query Frame = -1

Query:  110 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWW----------SRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGEHK 547
            Y+IEATCHAP     Y EI+RVLKPG  + +YEW  T+ F  +N+EH +I   IE GD LP +R      E  K+ GFEV+ E+DLA+    PW+          S  ++  +  +    +V +L  + +AP+G+  V   L + A+ L  GG  +
Sbjct:  167 YAIEATCHAPDAYGCYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRRE 322

[Close]

BLAST of GH720834 vs. TAIR peptide
Match: AT5G13710.1 (| Symbols: SMT1, CPH | sterol methyltransferase 1 | chr5:4424048-4426866 REVERSE LENGTH=336)

HSP 1 Score: 106.686 bits (265), Expect = 8.927e-24
Identity = 58/156 (37.18%), Postives = 85/156 (54.49%), Query Frame = -1

Query:  110 YSIEATCHAPKLEEVYAEIFRVLKPGALYVSYEWVTTEKFISENSEHVEIIQGIERGDALPGLRSYADIAEIAKKVGFEVVKERDLAQPPAEPWW----------SRLKMGRLAYWRNHVVVTVLSALGIAPKGTVDVHEMLFKTADYLTRGGEHK 547
            Y+IEATCHAP     Y EI+RVLKPG  + +YEW  T+ F  +N+EH +I   IE GD LP +R      E  K+ GFEV+ E+DLA+    PW+          S  ++  +  +    +V +L  + +AP+G+  V   L + A+ L  GG  +
Sbjct:  167 YAIEATCHAPDAYGCYKEIYRVLKPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSFRLTAVGRFITKNMVKILEYIRLAPQGSQRVSNFLEQAAEGLVDGGRRE 322

The following BLAST results are available for this feature:

BLAST of GH720834 vs. TrEMBL
BLAST of GH720834 vs. SwissProt
BLAST of GH720834 vs. TAIR peptide

BLAST of GH720834 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7KIB3_ARALY	2.241e-70	85.42	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
D2D5G3_SOYBN	2.241e-70	86.81	Sterol 24-C methyltransferase 2-1 OS=Glycine max G...	[more]
D2D5G4_SOYBN	2.927e-70	87.50	Sterol 24-C methyltransferase 2-2 OS=Glycine max G...	[more]
B9R8F7_RICCO	2.927e-70	86.11	S-adenosyl-l-methionine:delta24-sterol-C-methyltra...	[more]
A9P9Q4_POPTR	8.516e-70	84.72	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PE86_POPTR	1.897e-69	84.72	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B0FDL8_GOSHI	2.478e-69	83.33	24-sterol C-methyltransferase OS=Gossypium hirsutu...	[more]
B0FDL7_GOSHI	7.209e-69	83.33	24-sterol C-methyltransferase OS=Gossypium hirsutu...	[more]
D7KT96_ARALY	1.041e-67	82.64	S-adenosyl-methionine-sterol-C-methyltransferase 3...	[more]
A5BH10_VITVI	1.963e-66	80.56	Putative uncharacterized protein OS=Vitis vinifera...	[more]

BLAST of GH720834 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
SMT2_ARATH	5.720e-72	86.11	24-methylenesterol C-methyltransferase 2 OS=Arabid...	[more]
SMT3B_ARATH	1.010e-68	81.94	24-methylenesterol C-methyltransferase 3 OS=Arabid...	[more]
SMT2_ORYSJ	1.786e-57	72.22	24-methylenesterol C-methyltransferase 2 OS=Oryza ...	[more]
ERG6_KLULA	8.093e-26	41.46	Sterol 24-C-methyltransferase OS=Kluyveromyces lac...	[more]
ERG6_PNECA	2.604e-24	37.35	Sterol 24-C-methyltransferase OS=Pneumocystis cari...	[more]
ERG6_YEAST	1.688e-23	41.57	Sterol 24-C-methyltransferase OS=Saccharomyces cer...	[more]
ERG6_CANGA	1.688e-23	39.02	Sterol 24-C-methyltransferase OS=Candida glabrata ...	[more]
SMT1_ORYSJ	4.910e-23	37.72	Cycloartenol-C-24-methyltransferase 1 OS=Oryza sat...	[more]
ERG6_YARLI	4.910e-23	39.63	Sterol 24-C-methyltransferase OS=Yarrowia lipolyti...	[more]
SMT1_ARATH	8.375e-23	37.18	Cycloartenol-C-24-methyltransferase OS=Arabidopsis...	[more]

BLAST of GH720834 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT1G20330.1	6.097e-73	86.11	\| Symbols: SMT2, CVP1, FRL1 \| sterol methyltransfe...	[more]
AT1G76090.1	1.076e-69	81.94	\| Symbols: SMT3 \| sterol methyltransferase 3 \| chr...	[more]
AT5G13710.2	8.927e-24	37.18	\| Symbols: SMT1, CPH \| sterol methyltransferase 1 ...	[more]
AT5G13710.1	8.927e-24	37.18	\| Symbols: SMT1, CPH \| sterol methyltransferase 1 ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013705	Sterol methyltransferase C-terminal	PFAM	PF08498	Sterol_MT_C	coord: 104..152 score: 3.4
None	No IPR available	GENE3D	3.40.50.150	no description	coord: 7..151 score: 1.8
None	No IPR available	PANTHER	PTHR10108	METHYLTRANSFERASE	coord: 7..130 score: 2.5
None	No IPR available	PANTHER	PTHR10108:SF21	S-ADENOSYL-L-METHIONINE:DELTA24-STEROL-C-METHYLTRANSFERASE	coord: 7..130 score: 2.5
None	No IPR available	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 7..151 score: 1.4

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720834 ID=GH720834; Name=GH720834; organism=Pisum sativum; type=EST; length=565bp
TTCGAGCGGCCGCCCGGGCAGGTACAACCTGACAGGAAGAGAAAGATGCC
ATTTTATATTCTGCGGAGACAGGGTTGAAGTGATGAATTGGCTCCCACTA
ATCTTCTTTCTTATGTTCACCCCCTCTGGTCAAATAGTCAGCAGTCTTGA
ACAGCATCTCATGAACATCAACCGTCCCTTTGGGCGCTATTCCCAATGCA
GACAGAACAGTTACCACAACATGGTTACGCCAATAAGCAAGCCGACCCAT
TTTCAAACGGCTCCACCACGGCTCAGCCGGTGGCTGAGCCAGATCCCGCT
CCTTAACAACCTCAAACCCAACTTTCTTAGCTATTTCAGCTATATCAGCG
TAGCTTCTAAGCCCAGGCAAAGCATCCCCTCTTTCAATTCCTTGAATAAT
CTCAACATGTTCTGAATTTTCAGATATGAATTTCTCTGTTGTGACCCATT
CATATGAAACGTAAAGAGCGCCGGGTTTCAAAACCCTAAAAATTTCAGCG
TAAACTTCTTCAAGCTTAGGCGCGTGGCACGTCGCTTCGATTGAGTACCT
CGGCCGCGACCACGC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006694	steroid biosynthetic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0008168	methyltransferase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR013705	Sterol_MeTrfase_C

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