CD859142

EST Overview


Name	CD859142
Unique Name	CD859142
Type	EST
Organism	Pisum sativum (pea)
Sequence length	630

Libraries


Library Name	Type
LIBEST_013999 CNI	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of CD859142 vs. TrEMBL
Match: C6TGF2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 311.227 bits (796), Expect = 3.837e-89
Identity = 151/185 (81.62%), Postives = 163/185 (88.11%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAGRGEYDAVFMXXXXXXXXXXXXPDPPQFLYDEDEYQDIFLSDAIF 607
            LGGGSSNAATALWAANQFSGCPA+EKELQEWSSE+GSD+PFFFSQGAAYCTGRGE+VQNIPP VS ++PMVLIKP EACSTAEVYK LRLDQTSNVDP  LLE+I   GISQDVCINDLEPPAFEVLPSLKRLKQRI+AAGRGEYDAVFM            PDPPQF+YD+DEY+D+FLSDA F
Sbjct:  185 LGGGSSNAATALWAANQFSGCPASEKELQEWSSEVGSDIPFFFSQGAAYCTGRGEVVQNIPPPVSLDVPMVLIKPPEACSTAEVYKCLRLDQTSNVDPLTLLEKISKIGISQDVCINDLEPPAFEVLPSLKRLKQRISAAGRGEYDAVFMSGSGSTIVGIGSPDPPQFVYDDDEYKDVFLSDAYF 369

HSP 2 Score: 40.0466 bits (92), Expect = 3.837e-89
Identity = 15/18 (83.33%), Postives = 18/18 (100.00%), Query Frame = 3

Query:    3 EKFFWIHLDKKVPTGAGL 56
            +K+FWIHLDK+VPTGAGL
Sbjct:  168 DKYFWIHLDKRVPTGAGL 185

HSP 3 Score: 23.8682 bits (50), Expect = 3.837e-89
Identity = 8/10 (80.00%), Postives = 9/10 (90.00%), Query Frame = 1

Query:  601 YFLTREENEW 630
            YFLTRE N+W
Sbjct:  368 YFLTREANQW 377

[Close]

BLAST of CD859142 vs. TrEMBL
Match: B3GEM3_POPTR (Putative chloroplast 4-(Cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (Fragment) OS=Populus trichocarpa GN=CMK PE=2 SV=1)

HSP 1 Score: 293.508 bits (750), Expect = 1.155e-82
Identity = 144/185 (77.84%), Postives = 157/185 (84.86%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAGRGEYDAVFMXXXXXXXXXXXXPDPPQFLYDEDEYQDIFLSDAIF 607
            LGGGSSNAATALWAANQFSGC ATEKELQEWSSEIGSD+PFFFS GAAYCTGRGEIVQ+IP LV  + PMVLIKP +ACSTAEVYKR +LD+TS  DP  LLE+I  +GISQDVCINDLEPPAFEVLPSLKRLKQRI AAGRG+YDAVFM            PDPPQF+YDEDEYQD+F+S+A F
Sbjct:  129 LGGGSSNAATALWAANQFSGCLATEKELQEWSSEIGSDIPFFFSHGAAYCTGRGEIVQDIPSLVPLDKPMVLIKPPQACSTAEVYKRFQLDKTSQADPLTLLEKISRNGISQDVCINDLEPPAFEVLPSLKRLKQRIAAAGRGQYDAVFMSGSGSTIVGIGSPDPPQFIYDEDEYQDVFVSEANF 313

HSP 2 Score: 38.5058 bits (88), Expect = 1.155e-82
Identity = 15/18 (83.33%), Postives = 16/18 (88.89%), Query Frame = 3

Query:    3 EKFFWIHLDKKVPTGAGL 56
            + FFWIHLDK VPTGAGL
Sbjct:  112 DNFFWIHLDKNVPTGAGL 129

[Close]

BLAST of CD859142 vs. TrEMBL
Match: B9GEN2_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_181114 PE=3 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 1.966e-82
Identity = 143/185 (77.30%), Postives = 157/185 (84.86%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAGRGEYDAVFMXXXXXXXXXXXXPDPPQFLYDEDEYQDIFLSDAIF 607
            LGGGSSNAATALWAANQFSGC ATEKELQEWSSEIGSD+PFFFS GAAYCTGRGEIVQ+IP  VSS IPMVL+KP ++CSTAEVYK  +LD+TS  DP  LLE+I  +GISQDVCINDLEPPAFEVLPSLKRLKQRI AAGRG+YDAVFM            PDPPQF+YDEDEYQD+F+S+A F
Sbjct:  122 LGGGSSNAATALWAANQFSGCLATEKELQEWSSEIGSDIPFFFSHGAAYCTGRGEIVQDIPSPVSSGIPMVLMKPPQSCSTAEVYKCFQLDKTSQADPLTLLEKISRNGISQDVCINDLEPPAFEVLPSLKRLKQRIAAAGRGQYDAVFMSGSGSTIVGIGSPDPPQFIYDEDEYQDVFVSEANF 306

HSP 2 Score: 40.4318 bits (93), Expect = 1.966e-82
Identity = 16/18 (88.89%), Postives = 17/18 (94.44%), Query Frame = 3

Query:    3 EKFFWIHLDKKVPTGAGL 56
            + FFWIHLDKKVPTGAGL
Sbjct:  105 DNFFWIHLDKKVPTGAGL 122

[Close]

BLAST of CD859142 vs. TrEMBL
Match: A5A5H5_SALMI (4-diphosphocytidyl-2-C-methyl-D-erythritol kinase OS=Salvia miltiorrhiza PE=2 SV=1)

HSP 1 Score: 291.197 bits (744), Expect = 3.341e-82
Identity = 137/185 (74.05%), Postives = 158/185 (85.41%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAGRGEYDAVFMXXXXXXXXXXXXPDPPQFLYDEDEYQDIFLSDAIF 607
            LGGGSSNAATALWAANQFSGC A+EK+LQEWS EIGSD+PFFFS GAAYCTGRGE+V++IPP V  ++PMVLIKPQEAC T EVYKRLR++QTS +DP +LLE+I   GISQDVC+NDLEPPAFEV+PSLKRLKQRI AAGRG+YDAVFM            PDPPQF+YD+DEY+++FLSDA F
Sbjct:  189 LGGGSSNAATALWAANQFSGCVASEKDLQEWSGEIGSDIPFFFSHGAAYCTGRGEVVEDIPPPVPLDLPMVLIKPQEACPTGEVYKRLRMNQTSQIDPLVLLEKISKGGISQDVCVNDLEPPAFEVVPSLKRLKQRIAAAGRGQYDAVFMSGSGSTIVGVGSPDPPQFVYDDDEYKNVFLSDAKF 373

HSP 2 Score: 39.2762 bits (90), Expect = 3.341e-82
Identity = 15/18 (83.33%), Postives = 17/18 (94.44%), Query Frame = 3

Query:    3 EKFFWIHLDKKVPTGAGL 56
            + +FWIHLDKKVPTGAGL
Sbjct:  172 DNYFWIHLDKKVPTGAGL 189

[Close]

BLAST of CD859142 vs. TrEMBL
Match: B9HPT7_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_650358 PE=3 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 7.423e-82
Identity = 142/185 (76.76%), Postives = 157/185 (84.86%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAGRGEYDAVFMXXXXXXXXXXXXPDPPQFLYDEDEYQDIFLSDAIF 607
            LGGGSSNAATALWAANQFSGC ATEKELQEWSSEIGSD+PFFFS GAAYCTGRGEIVQ+IP LV  + PMVLIKP +ACSTAEVYKR +LD+TS  DP  LL++I  +GISQDVCINDLEPPAFE+LPSLKRLKQRI AAGRG+YDAVFM            PDPPQF+YDEDEYQD+F+S+A F
Sbjct:  122 LGGGSSNAATALWAANQFSGCLATEKELQEWSSEIGSDIPFFFSHGAAYCTGRGEIVQDIPSLVPLDKPMVLIKPPQACSTAEVYKRFQLDKTSQADPLTLLKKISRNGISQDVCINDLEPPAFELLPSLKRLKQRIAAAGRGQYDAVFMSGSGSTIVGIGSPDPPQFIYDEDEYQDVFVSEANF 306

HSP 2 Score: 38.5058 bits (88), Expect = 7.423e-82
Identity = 15/18 (83.33%), Postives = 16/18 (88.89%), Query Frame = 3

Query:    3 EKFFWIHLDKKVPTGAGL 56
            + FFWIHLDK VPTGAGL
Sbjct:  105 DNFFWIHLDKNVPTGAGL 122

[Close]

BLAST of CD859142 vs. TrEMBL
Match: A2TGW3_9LAMI (4-diphosphocytidyl-2-C-methyl-D-erythritol kinase OS=Picrorhiza kurrooa GN=IspE PE=2 SV=1)

HSP 1 Score: 287.73 bits (735), Expect = 3.651e-81
Identity = 138/185 (74.59%), Postives = 155/185 (83.78%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAGRGEYDAVFMXXXXXXXXXXXXPDPPQFLYDEDEYQDIFLSDAIF 607
            LGGGSSNAATALWAANQFSGC A+EKELQEWS EIGSD+PFFFS GAAYCTGRGE+++NIPP    ++PMVLIKP EAC+TAEVYK LRLD  S +DP  LLE++LS+GISQDVC+NDLE PAFEVLPSLKRLKQRI AAGRG+YDAVFM            PDPP FLYDEDEY+D+FLS+A F
Sbjct:  193 LGGGSSNAATALWAANQFSGCIASEKELQEWSGEIGSDIPFFFSHGAAYCTGRGEVIENIPPPTPFDVPMVLIKPPEACATAEVYKCLRLDLMSKIDPLTLLEKVLSNGISQDVCVNDLEIPAFEVLPSLKRLKQRIAAAGRGQYDAVFMSGSGSTIVGVGSPDPPHFLYDEDEYKDVFLSEASF 377

HSP 2 Score: 39.2762 bits (90), Expect = 3.651e-81
Identity = 15/18 (83.33%), Postives = 17/18 (94.44%), Query Frame = 3

Query:    3 EKFFWIHLDKKVPTGAGL 56
            + +FWIHLDKKVPTGAGL
Sbjct:  176 DNYFWIHLDKKVPTGAGL 193

[Close]

BLAST of CD859142 vs. TrEMBL
Match: A4USB3_NICBE (4-diphosphocytidyl-2-C-methyl-D-erythritol kinase OS=Nicotiana benthamiana PE=2 SV=1)

HSP 1 Score: 285.034 bits (728), Expect = 6.211e-81
Identity = 138/185 (74.59%), Postives = 155/185 (83.78%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAGRGEYDAVFMXXXXXXXXXXXXPDPPQFLYDEDEYQDIFLSDAIF 607
            LGGGSSNAATALWAANQFSGC ATEKELQEWS EIGSD+ FFFS GAAYCTGRGE+VQ+IP  +  +IPMVLIKPQ+ CSTAEVYKR  LDQTSNVDP  LLE+I +SGISQDVC+NDLEPPAFEVLPSLKRLKQR+ AA RG+YDAVF             PDPPQF+YD++EY+D+FLS+A F
Sbjct:  194 LGGGSSNAATALWAANQFSGCLATEKELQEWSGEIGSDISFFFSHGAAYCTGRGEVVQDIPSPIPFDIPMVLIKPQQECSTAEVYKRFLLDQTSNVDPLSLLEKIKTSGISQDVCVNDLEPPAFEVLPSLKRLKQRVIAAXRGQYDAVFXSGSGSTIVGVGSPDPPQFVYDDEEYKDVFLSEASF 378

HSP 2 Score: 41.2022 bits (95), Expect = 6.211e-81
Identity = 16/18 (88.89%), Postives = 18/18 (100.00%), Query Frame = 3

Query:    3 EKFFWIHLDKKVPTGAGL 56
            +K+FWIHLDKKVPTGAGL
Sbjct:  177 DKYFWIHLDKKVPTGAGL 194

[Close]

BLAST of CD859142 vs. TrEMBL
Match: D7T1C6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_9.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00037382001 PE=4 SV=1)

HSP 1 Score: 283.878 bits (725), Expect = 6.790e-80
Identity = 138/185 (74.59%), Postives = 155/185 (83.78%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAGRGEYDAVFMXXXXXXXXXXXXPDPPQFLYDEDEYQDIFLSDAIF 607
            LGGGSSNAATALWAANQFSGC ATEKELQ+WSSEIGSD+PFFFS GAA+CTGRGE+V++I   +  +IPMVL+KP +ACSTAEVYK LRLDQTS VDP  LLE+I  + ISQDVCINDLEPPAFEVLPSLKRLKQRI AA RG+YDAVFM            PDPPQF+YD+DEYQD+FLS+A F
Sbjct:  183 LGGGSSNAATALWAANQFSGCLATEKELQQWSSEIGSDIPFFFSNGAAFCTGRGEVVEDISLPIPLDIPMVLVKPPQACSTAEVYKHLRLDQTSKVDPITLLEKISKNRISQDVCINDLEPPAFEVLPSLKRLKQRIIAASRGQYDAVFMSGSGSTIVGIGSPDPPQFIYDDDEYQDVFLSEASF 367

HSP 2 Score: 38.891 bits (89), Expect = 6.790e-80
Identity = 14/18 (77.78%), Postives = 17/18 (94.44%), Query Frame = 3

Query:    3 EKFFWIHLDKKVPTGAGL 56
            + +FW+HLDKKVPTGAGL
Sbjct:  166 DNYFWVHLDKKVPTGAGL 183

[Close]

BLAST of CD859142 vs. TrEMBL
Match: A9ZN11_HEVBR (4-(Cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase OS=Hevea brasiliensis GN=HbCMK PE=2 SV=1)

HSP 1 Score: 282.337 bits (721), Expect = 1.507e-79
Identity = 139/185 (75.14%), Postives = 156/185 (84.32%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAGRGEYDAVFMXXXXXXXXXXXXPDPPQFLYDEDEYQDIFLSDAIF 607
            LGGGSSNAATALWAANQFSG  ATEKEL EWSSEIGSD+ FFFS+GAAYCTGRGEIVQ+IP  V  ++PMVLIKPQEACSTAEVYKR RLD+TS +DP  LLE+I  +GISQDVCINDLEPPAFEVLPSLKRLKQRI AA RG+YDAVFM            PDPPQF+YD+D+Y+D+F+S+A F
Sbjct:  175 LGGGSSNAATALWAANQFSGGLATEKELLEWSSEIGSDISFFFSRGAAYCTGRGEIVQDIPTPVPLDLPMVLIKPQEACSTAEVYKRFRLDKTSQIDPLTLLEKISRNGISQDVCINDLEPPAFEVLPSLKRLKQRIIAASRGQYDAVFMSGSGSTIVGIGSPDPPQFIYDDDDYKDVFVSEANF 359

HSP 2 Score: 39.2762 bits (90), Expect = 1.507e-79
Identity = 15/18 (83.33%), Postives = 17/18 (94.44%), Query Frame = 3

Query:    3 EKFFWIHLDKKVPTGAGL 56
            + FFWIHLDK+VPTGAGL
Sbjct:  158 DNFFWIHLDKRVPTGAGL 175

[Close]

BLAST of CD859142 vs. TrEMBL
Match: B9SB47_RICCO (4-diphosphocytidyl-2-C-methyl-d-erythritol kinase, putative OS=Ricinus communis GN=RCOM_0784670 PE=3 SV=1)

HSP 1 Score: 279.256 bits (713), Expect = 2.925e-79
Identity = 138/185 (74.59%), Postives = 154/185 (83.24%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAGRGEYDAVFMXXXXXXXXXXXXPDPPQFLYDEDEYQDIFLSDAIF 607
            LGGGSSNAATALWAANQFSG  ATEK+L EWSSEIGSD+ FFFS+GAAYCTGRGEIVQ+IP  V  +IPMVLIKPQEAC T EVYKR RLDQTS VDP  LL++I  SGISQDVCINDLEPPAFEVLPSLKRLKQRI AA RG+Y+AVFM            PDPPQF+YD+D+Y+D+F+S+A F
Sbjct:  175 LGGGSSNAATALWAANQFSGGLATEKDLLEWSSEIGSDISFFFSRGAAYCTGRGEIVQDIPSPVPLDIPMVLIKPQEACPTGEVYKRFRLDQTSQVDPLTLLDKISKSGISQDVCINDLEPPAFEVLPSLKRLKQRIVAASRGQYNAVFMSGSGSTIVGIGSPDPPQFIYDDDDYKDVFVSEANF 359

HSP 2 Score: 40.4318 bits (93), Expect = 2.925e-79
Identity = 16/18 (88.89%), Postives = 17/18 (94.44%), Query Frame = 3

Query:    3 EKFFWIHLDKKVPTGAGL 56
            + FFWIHLDKKVPTGAGL
Sbjct:  158 DNFFWIHLDKKVPTGAGL 175

HSP 3 Score: 22.3274 bits (46), Expect = 2.925e-79
Identity = 8/9 (88.89%), Postives = 8/9 (88.89%), Query Frame = 1

Query:  604 FLTREENEW 630
            FLTRE NEW
Sbjct:  359 FLTREANEW 367

[Close]

BLAST of CD859142 vs. SwissProt
Match: ISPE_SOLLC (4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic/chromoplastic (Fragment) OS=Solanum lycopersicum GN=ISPE PE=1 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.500e-82
Identity = 138/185 (74.59%), Postives = 157/185 (84.86%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAGRGEYDAVFMXXXXXXXXXXXXPDPPQFLYDEDEYQDIFLSDAIF 607
            LGGGSSNAAT LWAANQFSGC ATEKELQEWS EIGSD+PFFFS GAAYCTGRGE+VQ+IP  +  +IPMVLIKPQ+ACSTAEVYKR +LD +S VDP  LLE+I +SGISQDVC+NDLEPPAFEVLPSLKRLKQR+ AAGRG+YDAVFM            PDPPQF+YD++EY+D+FLS+A F
Sbjct:  186 LGGGSSNAATTLWAANQFSGCVATEKELQEWSGEIGSDIPFFFSHGAAYCTGRGEVVQDIPSPIPFDIPMVLIKPQQACSTAEVYKRFQLDLSSKVDPLSLLEKISTSGISQDVCVNDLEPPAFEVLPSLKRLKQRVIAAGRGQYDAVFMSGSGSTIVGVGSPDPPQFVYDDEEYKDVFLSEASF 370

HSP 2 Score: 39.2762 bits (90), Expect = 1.500e-82
Identity = 15/18 (83.33%), Postives = 17/18 (94.44%), Query Frame = 3

Query:    3 EKFFWIHLDKKVPTGAGL 56
            + +FWIHLDKKVPTGAGL
Sbjct:  169 DNYFWIHLDKKVPTGAGL 186

[Close]

BLAST of CD859142 vs. SwissProt
Match: ISPE_MENPI (4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic OS=Mentha piperita GN=ISPE PE=1 SV=1)

HSP 1 Score: 285.034 bits (728), Expect = 1.257e-81
Identity = 136/185 (73.51%), Postives = 155/185 (83.78%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAGRGEYDAVFMXXXXXXXXXXXXPDPPQFLYDEDEYQDIFLSDAIF 607
            LGGGSSNAATALWAANQFSGC ATEK+LQEWS EIGSD+PFFFS GAAYCTGRGE+V++IPP V  ++ MVL+KPQEAC T EVYKRLRLDQTS++DP +LLE+I   GISQDVC+NDLEPPAFEV+PSLKRLKQRI AAGR +YDAVFM            PDPPQF+YD DEY++IF S+A F
Sbjct:  195 LGGGSSNAATALWAANQFSGCIATEKDLQEWSGEIGSDIPFFFSHGAAYCTGRGEVVEDIPPPVPRDLSMVLMKPQEACPTGEVYKRLRLDQTSDIDPLVLLEKISKGGISQDVCVNDLEPPAFEVVPSLKRLKQRIAAAGRSQYDAVFMSGSGSTIVGVGSPDPPQFVYDGDEYKNIFFSEAKF 379

HSP 2 Score: 39.6614 bits (91), Expect = 1.257e-81
Identity = 16/18 (88.89%), Postives = 17/18 (94.44%), Query Frame = 3

Query:    3 EKFFWIHLDKKVPTGAGL 56
            +K FWIHLDKKVPTGAGL
Sbjct:  178 DKHFWIHLDKKVPTGAGL 195

[Close]

BLAST of CD859142 vs. SwissProt
Match: ISPE_ARATH (4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic OS=Arabidopsis thaliana GN=ISPE PE=2 SV=1)

HSP 1 Score: 277.715 bits (709), Expect = 9.981e-80
Identity = 131/185 (70.81%), Postives = 155/185 (83.78%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAGRGEYDAVFMXXXXXXXXXXXXPDPPQFLYDEDEYQDIFLSDAIF 607
            LGGGSSNAATALWAAN+ +G   TE ELQ+WSSEIGSD+PFFFS GAAYCTGRGEIVQ++PP    ++PMVLIKP+EACSTAEVYKRLRLDQTSN++P  LLE + S+G+SQ +C+NDLEPPAF VLPSLKRLKQRI A+GRGEYDAVFM            PDPPQF+YD++EY+++FLS+A F
Sbjct:  170 LGGGSSNAATALWAANELNGGLVTENELQDWSSEIGSDIPFFFSHGAAYCTGRGEIVQDLPPPFPLDLPMVLIKPREACSTAEVYKRLRLDQTSNINPLTLLENVTSNGVSQSICVNDLEPPAFSVLPSLKRLKQRIIASGRGEYDAVFMSGSGSTIIGIGSPDPPQFIYDDEEYKNVFLSEANF 354

HSP 2 Score: 40.4318 bits (93), Expect = 9.981e-80
Identity = 16/17 (94.12%), Postives = 17/17 (100.00%), Query Frame = 3

Query:    6 KFFWIHLDKKVPTGAGL 56
            +FFWIHLDKKVPTGAGL
Sbjct:  154 RFFWIHLDKKVPTGAGL 170

HSP 3 Score: 21.557 bits (44), Expect = 9.981e-80
Identity = 7/9 (77.78%), Postives = 8/9 (88.89%), Query Frame = 1

Query:  604 FLTREENEW 630
            F+TRE NEW
Sbjct:  354 FMTREANEW 362

[Close]

BLAST of CD859142 vs. SwissProt
Match: ISPE_PARUW (4-diphosphocytidyl-2-C-methyl-D-erythritol kinase OS=Protochlamydia amoebophila (strain UWE25) GN=ispE PE=3 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 8.600e-29
Identity = 68/150 (45.33%), Postives = 89/150 (59.33%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAGRGEYDAVFM 502
            LGGGSSNAAT LWA NQ +G   T +EL +W SEIG+D+PFFFS+G A+CTGRGE V ++ PL   +I   ++KP    ST EVYK L   Q +  +          +   +    NDLE  AFE+ P LK LK  + ++G   +D V M
Sbjct:  109 LGGGSSNAATTLWACNQLAGEIVTTEELMQWGSEIGADIPFFFSKGTAHCTGRGECVNSLEPLAHCKI--WIVKPPFGLSTPEVYKHLNFSQPNENNNDY-------ASFKEKPYFNDLEASAFEIKPELKILKNTLLSSG---FDTVLM 246

HSP 2 Score: 23.8682 bits (50), Expect = 8.600e-29
Identity = 9/13 (69.23%), Postives = 12/13 (92.31%), Query Frame = 3

Query:   18 IHLDKKVPTGAGL 56
            IHLDK++P+ AGL
Sbjct:   97 IHLDKRLPSQAGL 109

[Close]

BLAST of CD859142 vs. SwissProt
Match: ISPE_DEHSC (4-diphosphocytidyl-2-C-methyl-D-erythritol kinase OS=Dehalococcoides sp. (strain CBDB1) GN=ispE PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.648e-15
Identity = 57/145 (39.31%), Postives = 73/145 (50.34%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKP---QEACSTAEVYKRLRLDQ-TSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAG 475
            LGG SS AA  L   N+  GC      L E  +E+GSDVPFF   G A   GRGE V  +P L  +++  VL+ P     A  TA +Y+ LR D  TS      LLE I    +S  +C N  E  AFE+ P L + + +   AG
Sbjct:   95 LGGDSSCAAAVLKGLNKLWGCGYPCWRLMEIGAELGSDVPFFIMGGTAMMEGRGETVTPLPTL--TQMWAVLLVPALDMPADKTAALYRNLRPDSFTSGEISDKLLESICQGKLSLSLCFNAFEKVAFELFPELVKYRWQFLEAG 237

[Close]

BLAST of CD859142 vs. SwissProt
Match: ISPE_DEHSB (4-diphosphocytidyl-2-C-methyl-D-erythritol kinase OS=Dehalococcoides sp. (strain BAV1) GN=ispE PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.648e-15
Identity = 57/145 (39.31%), Postives = 73/145 (50.34%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKP---QEACSTAEVYKRLRLDQ-TSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAG 475
            LGG SS AA  L   N+  GC      L E  +E+GSDVPFF   G A   GRGE V  +P L  +++  VL+ P     A  TA +Y+ LR D  TS      LLE I    +S  +C N  E  AFE+ P L + + +   AG
Sbjct:   95 LGGDSSCAAAVLKGLNKLWGCGYPCWRLMEIGAELGSDVPFFIMGGTAMMEGRGETVTPLPTL--TQMWAVLLVPALDMPADKTAALYRNLRPDSFTSGEISDKLLESICQGKLSLSLCFNAFEKVAFELFPELVKYRWQFLEAG 237

[Close]

BLAST of CD859142 vs. SwissProt
Match: ISPE_DESHY (4-diphosphocytidyl-2-C-methyl-D-erythritol kinase OS=Desulfitobacterium hafniense (strain Y51) GN=ispE PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.352e-14
Identity = 50/141 (35.46%), Postives = 87/141 (61.70%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLD-QTSNVDPSILLERILSSGISQD---VCINDLEPPAFEVLPSLKRLKQRI 463
            LGGGS++AA AL A N+    P TE+EL+ +++++G+DV F    G  + TG GE ++ +P   + +I +VLIKP +  +TAE Y+    + + S++D +   E  L+SG ++    +  NDLEP + ++LP +  +K+ +
Sbjct:   98 LGGGSADAAAALQALNKLFKEPYTEEELKSFAAQLGADVAFCLKGGTQWATGVGEELKGLPH--APKINLVLIKPDQGVNTAEAYRAFDQEGKFSHLDYAGWQE-ALASGRAESLIPLLYNDLEPASMKLLPEIAWVKEEL 235

[Close]

BLAST of CD859142 vs. SwissProt
Match: ISPE_DESHD (4-diphosphocytidyl-2-C-methyl-D-erythritol kinase OS=Desulfitobacterium hafniense (strain DCB-2 / DSM 10664) GN=ispE PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.352e-14
Identity = 50/141 (35.46%), Postives = 87/141 (61.70%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLD-QTSNVDPSILLERILSSGISQD---VCINDLEPPAFEVLPSLKRLKQRI 463
            LGGGS++AA AL A N+    P TE+EL+ +++++G+DV F    G  + TG GE ++ +P   + +I +VLIKP +  +TAE Y+    + + S++D +   E  L+SG ++    +  NDLEP + ++LP +  +K+ +
Sbjct:   98 LGGGSADAAAALQALNKLFKEPYTEEELKSFAAQLGADVAFCLKGGTQWATGVGEELKGLPH--APKINLVLIKPDQGVNTAEAYRAFDQEGKFSHLDYAGWQE-ALASGRAESLIPLLYNDLEPASMKLLPEIAWVKEEL 235

[Close]

BLAST of CD859142 vs. SwissProt
Match: ISPE_LISMO (4-diphosphocytidyl-2-C-methyl-D-erythritol kinase OS=Listeria monocytogenes GN=ispE PE=3 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 2.307e-14
Identity = 54/143 (37.76%), Postives = 73/143 (51.05%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCI--NDLEPPAFEVLPSLKRLKQRITAAG 475
            L GGSS+AA AL   N       + +EL E SSEIGSD+ F    G A  TGRGE +  +P +    I  VL KP  + ST  +YK L++D   + D   ++E I +  +        N LE    E  P +KR+K R+ A G
Sbjct:   99 LAGGSSDAAAALKGLNVIWELGLSIEELAEISSEIGSDIAFCVYGGTALATGRGEKISALPNIPGCWI--VLAKPSISVSTPTIYKELQVDNVEHPDTQKMIESIKNGDLDGIFAATGNVLESVTLEKNPQVKRIKDRMLAFG 239

[Close]

BLAST of CD859142 vs. SwissProt
Match: ISPE_LISMF (4-diphosphocytidyl-2-C-methyl-D-erythritol kinase OS=Listeria monocytogenes serotype 4b (strain F2365) GN=ispE PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 3.012e-14
Identity = 54/143 (37.76%), Postives = 73/143 (51.05%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCI--NDLEPPAFEVLPSLKRLKQRITAAG 475
            L GGSS+AA AL   N       + +EL E SSEIGSD+ F    G A  TGRGE +  +P +    I  VL KP  + ST  +YK L++D   + D   ++E I +  +        N LE    E  P +KR+K R+ A G
Sbjct:   99 LAGGSSDAAAALKGLNVIWELGLSIEELAEISSEIGSDIAFCVYGGTALATGRGEKISALPNIPGCWI--VLAKPSISVSTPTIYKELQVDNVEHPDTQKMIESIKNGDLDGIFASTGNVLESVTLEKNPQVKRIKDRMLAFG 239

[Close]

BLAST of CD859142 vs. TAIR peptide
Match: AT2G26930.1 (| Symbols: ATCDPMEK, PDE277, ISPE, CDPMEK | 4-(cytidine 5'-phospho)-2-C-methyl-D-erithritol kinase | chr2:11491829-11494229 REVERSE LENGTH=383)

HSP 1 Score: 277.715 bits (709), Expect = 1.028e-80
Identity = 131/185 (70.81%), Postives = 155/185 (83.78%), Query Frame = 2

Query:   53 LGGGSSNAATALWAANQFSGCPATEKELQEWSSEIGSDVPFFFSQGAAYCTGRGEIVQNIPPLVSSEIPMVLIKPQEACSTAEVYKRLRLDQTSNVDPSILLERILSSGISQDVCINDLEPPAFEVLPSLKRLKQRITAAGRGEYDAVFMXXXXXXXXXXXXPDPPQFLYDEDEYQDIFLSDAIF 607
            LGGGSSNAATALWAAN+ +G   TE ELQ+WSSEIGSD+PFFFS GAAYCTGRGEIVQ++PP    ++PMVLIKP+EACSTAEVYKRLRLDQTSN++P  LLE + S+G+SQ +C+NDLEPPAF VLPSLKRLKQRI A+GRGEYDAVFM            PDPPQF+YD++EY+++FLS+A F
Sbjct:  170 LGGGSSNAATALWAANELNGGLVTENELQDWSSEIGSDIPFFFSHGAAYCTGRGEIVQDLPPPFPLDLPMVLIKPREACSTAEVYKRLRLDQTSNINPLTLLENVTSNGVSQSICVNDLEPPAFSVLPSLKRLKQRIIASGRGEYDAVFMSGSGSTIIGIGSPDPPQFIYDDEEYKNVFLSEANF 354

HSP 2 Score: 40.4318 bits (93), Expect = 1.028e-80
Identity = 16/17 (94.12%), Postives = 17/17 (100.00%), Query Frame = 3

Query:    6 KFFWIHLDKKVPTGAGL 56
            +FFWIHLDKKVPTGAGL
Sbjct:  154 RFFWIHLDKKVPTGAGL 170

HSP 3 Score: 21.557 bits (44), Expect = 1.028e-80
Identity = 7/9 (77.78%), Postives = 8/9 (88.89%), Query Frame = 1

Query:  604 FLTREENEW 630
            F+TRE NEW
Sbjct:  354 FMTREANEW 362

The following BLAST results are available for this feature:

BLAST of CD859142 vs. TrEMBL
BLAST of CD859142 vs. SwissProt
BLAST of CD859142 vs. TAIR peptide

BLAST of CD859142 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TGF2_SOYBN	3.837e-89	81.62	Putative uncharacterized protein OS=Glycine max PE...	[more]
B3GEM3_POPTR	1.155e-82	77.84	Putative chloroplast 4-(Cytidine 5'-diphospho)-2-C...	[more]
B9GEN2_POPTR	1.966e-82	77.30	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
A5A5H5_SALMI	3.341e-82	74.05	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase ...	[more]
B9HPT7_POPTR	7.423e-82	76.76	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
A2TGW3_9LAMI	3.651e-81	74.59	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase ...	[more]
A4USB3_NICBE	6.211e-81	74.59	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase ...	[more]
D7T1C6_VITVI	6.790e-80	74.59	Whole genome shotgun sequence of line PN40024, sca...	[more]
A9ZN11_HEVBR	1.507e-79	75.14	4-(Cytidine 5'-diphospho)-2-C-methyl-D-erythritol ...	[more]
B9SB47_RICCO	2.925e-79	74.59	4-diphosphocytidyl-2-C-methyl-d-erythritol kinase,...	[more]

BLAST of CD859142 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
ISPE_SOLLC	1.500e-82	74.59	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase,...	[more]
ISPE_MENPI	1.257e-81	73.51	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase,...	[more]
ISPE_ARATH	9.981e-80	70.81	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase,...	[more]
ISPE_PARUW	8.600e-29	45.33	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase ...	[more]
ISPE_DEHSC	4.648e-15	39.31	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase ...	[more]
ISPE_DEHSB	4.648e-15	39.31	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase ...	[more]
ISPE_DESHY	1.352e-14	35.46	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase ...	[more]
ISPE_DESHD	1.352e-14	35.46	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase ...	[more]
ISPE_LISMO	2.307e-14	37.76	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase ...	[more]
ISPE_LISMF	3.012e-14	37.76	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase ...	[more]

BLAST of CD859142 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT2G26930.1	1.028e-80	70.81	\| Symbols: ATCDPMEK, PDE277, ISPE, CDPMEK \| 4-(cyt...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004424	4-diphosphocytidyl-2C-methyl-D-erythritol kinase	PANTHER	PTHR20861:SF2	4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE	coord: 8..176 score: 5.1
IPR013750	GHMP kinase, C-terminal	PFAM	PF08544	GHMP_kinases_C	coord: 128..177 score: 8.9
IPR014721	Ribosomal protein S5 domain 2-type fold, subgroup	GENE3D	3.30.230.10	no description	coord: 8..67 score: 4.2
IPR020568	Ribosomal protein S5 domain 2-type fold	SUPERFAMILY	54211	Ribosomal protein S5 domain 2-like	coord: 8..67 score: 9
None	No IPR available	PANTHER	PTHR20861	HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE	coord: 8..176 score: 5.1
None	No IPR available	SEG	seg	seg	coord: 158..169 score
None	No IPR available	SUPERFAMILY	55060	GHMP Kinase, C-terminal domain	coord: 72..192 score: 6.2
None	No IPR available	SEG	seg	seg	coord: 19..33 score
None	No IPR available	SEG	seg	seg	coord: 30..46 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD859142 ID=CD859142; Name=CD859142; organism=Pisum sativum; type=EST; length=630bp
TTGAAAAGTTCTTTTGGATTCATTTGGATAAAAAGGTTCCCACTGGGGCA
GGCTTGGTGGTGGAAGTAGTAATGCTGCGACTGCACTATGGGCAGCAAAC
CAATTCAGTGGGTGTCCTGCCACAGAGAAAGAACTCCAAGAATGGTCGAG
TGAGATTGGTTCAGATGTTCCCTTCTTTTTCTCTCAGGGAGCAGCCTATT
GCACTGGTCGAGGTGAGATTGTTCAGAATATTCCCCCACTGGTATCTTCG
GAAATTCCAATGGTTCTCATTAAGCCTCAAGAGGCGTGTTCAACTGCAGA
AGTCTACAAGCGTCTACGGTTGGACCAGACTAGTAATGTTGACCCGTCAA
TATTGCTGGAGAGAATCTTAAGCAGTGGTATATCTCAAGATGTTTGCATT
AATGATTTAGAACCTCCGGCATTTGAAGTTCTCCCTTCTCTCAAACGGCT
AAAACAGCGAATTACGGCAGCTGGTCGTGGAGAGTATGATGCTGTCTTCA
TGTCAGGAAGTGGAAGCACTATTGTTGGAATTGGTTCACCTGATCCTCCA
CAATTTCTTTATGACGAGGATGAATACCAAGACATATTCTTGTCAGATGC
TATTTTCTCACCAGGGAGGAAAATGAGTGG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0016114	terpenoid biosynthetic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0050515	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR004424	IspE
IPR013750	GHMP_kinase_C_dom
IPR014721	Ribsml_uS5_D2-typ_fold_subgr
IPR020568	Ribosomal_Su5_D2-typ_SF

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