CD860256

EST Overview


Name	CD860256
Unique Name	CD860256
Type	EST
Organism	Pisum sativum (pea)
Sequence length	439

Libraries


Library Name	Type
LIBEST_014002 PW	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of CD860256 vs. TrEMBL
Match: Q42810_SOYBN (GmCK2p OS=Glycine max PE=2 SV=1)

HSP 1 Score: 214.927 bits (546), Expect = 2.035e-54
Identity = 101/123 (82.11%), Postives = 110/123 (89.43%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSEGKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLLDTPTIVSQNETVNGSLP 369
            DLANHF EM ADYH+DTPHVLDY+KYP LEERQRFIR YLSSEG KP NAKVNQ+  A EKYTLANHLFWGLWGLISSYVNKI+FDYKEYARQRFQQY +RK TLLD+P+IVS +ETVNG +P
Sbjct:  237 DLANHFCEMVADYHSDTPHVLDYTKYPGLEERQRFIRNYLSSEGNKPSNAKVNQLAKAAEKYTLANHLFWGLWGLISSYVNKIDFDYKEYARQRFQQYWIRKPTLLDSPSIVSLDETVNGLMP 359

[Close]

BLAST of CD860256 vs. TrEMBL
Match: C6TJK8_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 209.92 bits (533), Expect = 6.547e-53
Identity = 100/122 (81.97%), Postives = 107/122 (87.70%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSEGKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLLDTPTIVSQNETVNGSL 366
            DLANHF EM ADYH+DTPHVLDY KYP LEERQRFIR YLSSE  KP NAKVNQ+V A EKYTLANHLFWGLWGLISSYVNKI+FDYKEY RQRFQQY +RK TLLD+P+IVS +ETVNG L
Sbjct:  237 DLANHFCEMVADYHSDTPHVLDYKKYPGLEERQRFIRNYLSSEDNKPSNAKVNQLVKAAEKYTLANHLFWGLWGLISSYVNKIDFDYKEYGRQRFQQYWIRKPTLLDSPSIVSLDETVNGLL 358

[Close]

BLAST of CD860256 vs. TrEMBL
Match: Q42809_SOYBN (GmCK1p OS=Glycine max PE=2 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 2.179e-48
Identity = 91/120 (75.83%), Postives = 104/120 (86.67%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSEGKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLLDTPTIVSQNETVNG 360
            DLANHF EM A+YH+D PHVLDYSKYP LEERQRF+  YLSSEGKKP N++V+Q+V   EKYTLANHLFWGLWGLISS+VN I+FDYKEYARQRFQQY L+K TLLD+P+IVSQ+   NG
Sbjct:  237 DLANHFCEMVANYHSDEPHVLDYSKYPGLEERQRFVYNYLSSEGKKPSNSEVDQLVNLAEKYTLANHLFWGLWGLISSHVNTIDFDYKEYARQRFQQYWLKKPTLLDSPSIVSQDGIANG 356

[Close]

BLAST of CD860256 vs. TrEMBL
Match: B7FI98_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 8.571e-45
Identity = 84/106 (79.25%), Postives = 95/106 (89.62%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSEGKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLL 318
            DLANHF EMAA+YHTDTPHVLDYSKYP+LEER+RFI  YLSSEG+KP  A+V+Q+   VEKYTLANHLFWGLWGLISSYVN I+FDYKEY+RQRF+QY L+K TLL
Sbjct:  237 DLANHFCEMAANYHTDTPHVLDYSKYPDLEERRRFIYTYLSSEGEKPSGAQVDQLANLVEKYTLANHLFWGLWGLISSYVNTIDFDYKEYSRQRFKQYHLKKPTLL 342

[Close]

BLAST of CD860256 vs. TrEMBL
Match: Q9AVR9_PEA (Choline kinase OS=Pisum sativum GN=ck1 PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 9.476e-44
Identity = 82/106 (77.36%), Postives = 93/106 (87.74%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSEGKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLL 318
            DLANHF EMAADYH+DTPHVLDY+KYP+LEER+RFI  YLSSEG+KP   +V Q+V  VEKYTLANHLFWGLWGLIS+YVN I+FDYKEY+RQRFQQY L+K  LL
Sbjct:  237 DLANHFCEMAADYHSDTPHVLDYTKYPDLEERRRFIHTYLSSEGEKPSEGEVEQLVNVVEKYTLANHLFWGLWGLISNYVNTIDFDYKEYSRQRFQQYWLKKVILL 342

[Close]

BLAST of CD860256 vs. TrEMBL
Match: A9PF05_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 3.726e-40
Identity = 78/117 (66.67%), Postives = 94/117 (80.34%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSEGKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLL----DTPTIVS 339
            D+ANHF EM ADYHTDTPH+LDYSKYP L+ERQRF+R YLSS G +P + +V Q++  VEKY LA+HLFWGLWG+IS +VN+I+FDY EYARQRF+QY LRK  LL     TP +VS
Sbjct:  236 DIANHFCEMTADYHTDTPHILDYSKYPGLDERQRFLRVYLSSSGGQPSDNEVEQLLENVEKYKLASHLFWGLWGIISEHVNEIDFDYMEYARQRFEQYWLRKPALLGSLGTTPDVVS 352

[Close]

BLAST of CD860256 vs. TrEMBL
Match: Q42811_SOYBN (GmCK3p (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 1.084e-39
Identity = 75/128 (58.59%), Postives = 99/128 (77.34%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSEGKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLLDTPTIVSQNETVNGSLPLASCV 384
            D+ANHF EMAA+YHT+ PH+LDY+KYP+ EERQRF++AYLS+ G++P +++V Q++  +EKYTLANHLFWG+WG+IS+ VN I+FDYKEYA+QRFQ+Y  RK  LL      S      G+  LAS V
Sbjct:  344 DIANHFCEMAANYHTEEPHILDYNKYPDFEERQRFVQAYLSTSGEQPSDSEVEQLLQEIEKYTLANHLFWGVWGIISAQVNTIDFDYKEYAKQRFQEYWARKPYLLINSEAPSPYNVPEGTGELASAV 471

[Close]

BLAST of CD860256 vs. TrEMBL
Match: B9SED6_RICCO (Choline/ethanolamine kinase, putative OS=Ricinus communis GN=RCOM_0962250 PE=4 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 1.084e-39
Identity = 74/121 (61.16%), Postives = 95/121 (78.51%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSEGKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLLDTPTIVSQNETVNGS 363
            D+ANHF EMAA+YH++TPHVLDYSKYP LEER+RF+  YL+S GKKP   KV ++    EKYTLANHLFWGLWG+IS YVNK++FDY EYARQRF+QY +RK+ +L +  + +    V+ +
Sbjct:  236 DIANHFCEMAANYHSETPHVLDYSKYPGLEERRRFVHVYLNSGGKKPSEDKVERLTDDAEKYTLANHLFWGLWGIISGYVNKLDFDYMEYARQRFEQYWVRKAEVLGSSGVHANGYVVDAT 356

[Close]

BLAST of CD860256 vs. TrEMBL
Match: B9GX91_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_413422 PE=4 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 3.154e-39
Identity = 74/106 (69.81%), Postives = 88/106 (83.02%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSEGKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLL 318
            D+ANHF EM ADYHTDTPH+LDYSKYP L+ERQRF+R YLSS G +P + +V Q++  VEKY LA+HLFWGLWG+IS +VN+I+FDY EYARQRF QY LRK  LL
Sbjct:  236 DIANHFCEMTADYHTDTPHILDYSKYPGLDERQRFLRVYLSSSGGQPSDNEVEQLIENVEKYKLASHLFWGLWGIISEHVNEIDFDYMEYARQRFGQYWLRKPALL 341

[Close]

BLAST of CD860256 vs. TrEMBL
Match: D7SXZ1_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_157.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00001426001 PE=4 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 4.120e-39
Identity = 74/106 (69.81%), Postives = 87/106 (82.08%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSEGKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLL 318
            DLANHF EM A+YHTDTPH+LDYSKYP+ EER RFIR YLSS G +P +++V ++    EKYTLANHLFWG+WG+IS +VN IEF+Y EYARQRFQQY LRK  LL
Sbjct:  234 DLANHFCEMVANYHTDTPHILDYSKYPDQEERHRFIRTYLSSAGNQPSDSEVEKLACDAEKYTLANHLFWGIWGIISGHVNTIEFNYMEYARQRFQQYWLRKPLLL 339

[Close]

BLAST of CD860256 vs. SwissProt
Match: EKI_NEMVE (Probable ethanolamine kinase OS=Nematostella vectensis GN=etnk PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 2.565e-15
Identity = 40/113 (35.40%), Postives = 66/113 (58.41%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSS----EGKK---PKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLL 318
            D+ANHF E A          +DYS YP+ + + +F+ +YL      +G+K   P + ++ ++   V ++ LA H FWG+WGL+ ++ ++I+FD+ EYA  R  +Y LRK   L
Sbjct:  238 DIANHFCEYAG------VDEVDYSLYPQKDHQVKFLESYLKRAMELQGEKDVNPSSREIEKLYVHVNQFALAAHFFWGVWGLVQAHYSEIDFDFLEYAITRLNEYYLRKEKFL 344

[Close]

BLAST of CD860256 vs. SwissProt
Match: EKI2_MOUSE (Ethanolamine kinase 2 OS=Mus musculus GN=Etnk2 PE=1 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 8.253e-14
Identity = 38/99 (38.38%), Postives = 57/99 (57.58%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSE-GKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQY 294
            D+ NHF+E A        +V+DYS+YP  E + +++R YL ++ G      +V ++   V K+ LA+H FW LW LI +  + I FD+  YA  RF QY
Sbjct:  279 DIGNHFNEFAG------VNVVDYSRYPARETQVQWLRYYLEAQKGTAASPREVERLYAQVNKFALASHFFWALWALIQNQYSTISFDFLRYAVIRFNQY 371

[Close]

BLAST of CD860256 vs. SwissProt
Match: CHKB_MOUSE (Choline/ethanolamine kinase OS=Mus musculus GN=Chkb PE=1 SV=3)

HSP 1 Score: 75.8702 bits (185), Expect = 8.253e-14
Identity = 40/118 (33.90%), Postives = 66/118 (55.93%), Query Frame = 1

Query:    1 DLANHFSEMAADY-HTDTP-HVLDYSKYPELEERQRFIRAYLSSEGK------KPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLLDTPT 330
            D+ NHF E   DY + + P +    + YP  E++  FIR YL+   K      + +  +  +++  + +Y+LA+H FWGLW  + + ++ IEF Y EYA+ RFQ Y  +K  L  +P+
Sbjct:  276 DIGNHFCEWVYDYTYEEWPFYKARPTDYPTREQQLHFIRHYLAEVQKGEILSEEEQKKREEELLLEISRYSLASHFFWGLWSTLQASMSTIEFGYLEYAQSRFQFYFQQKGQLTSSPS 393

[Close]

BLAST of CD860256 vs. SwissProt
Match: CHKB_HUMAN (Choline/ethanolamine kinase OS=Homo sapiens GN=CHKB PE=1 SV=3)

HSP 1 Score: 74.3294 bits (181), Expect = 2.401e-13
Identity = 41/113 (36.28%), Postives = 62/113 (54.87%), Query Frame = 1

Query:    1 DLANHFSEMAADY-HTDTP-HVLDYSKYPELEERQRFIRAYLSSEGKKPKNAKVNQ------VVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTL 315
            D+ NHF E   DY H + P +    + YP  E++  FIR YL+   K    ++  Q      ++  V +Y LA+H FWGLW ++ + ++ IEF Y +YA+ RFQ Y  +K  L
Sbjct:  276 DIGNHFCEWVYDYTHEEWPFYKARPTDYPTQEQQLHFIRHYLAEAKKGETLSQEEQRKLEEDLLVEVSRYALASHFFWGLWSILQASMSTIEFGYLDYAQSRFQFYFQQKGQL 388

[Close]

BLAST of CD860256 vs. SwissProt
Match: EKI1_HUMAN (Ethanolamine kinase 1 OS=Homo sapiens GN=ETNK1 PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.556e-12
Identity = 39/104 (37.50%), Postives = 57/104 (54.81%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSE------GKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQY 294
            D+ NHF+E A          +DYS YP+ E + +++RAYL +       G +    +V  +   V ++ LA+H FWGLW LI +  + IEFD+  YA  RF QY
Sbjct:  341 DIGNHFNEFAGVSD------VDYSLYPDRELQSQWLRAYLEAYKEFKGFGTEVTEKEVEILFIQVNQFALASHFFWGLWALIQAKYSTIEFDFLGYAIVRFNQY 438

[Close]

BLAST of CD860256 vs. SwissProt
Match: EKI2_RAT (Ethanolamine kinase 2 OS=Rattus norvegicus GN=Etnk2 PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 2.655e-12
Identity = 36/99 (36.36%), Postives = 55/99 (55.56%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSE-GKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQY 294
            D+ NHF+E A        + +DY +YP  E + +++R YL ++ G      +V ++   V K+ LA+H FW LW LI +  + I FD+  YA  RF QY
Sbjct:  279 DIGNHFNEFAG------VNEVDYCRYPAREIQLQWLRYYLEAQKGTAASPREVERLYAQVNKFALASHFFWALWALIQNQYSTINFDFLRYAVIRFNQY 371

[Close]

BLAST of CD860256 vs. SwissProt
Match: CHKB_RAT (Choline/ethanolamine kinase OS=Rattus norvegicus GN=Chkb PE=1 SV=3)

HSP 1 Score: 70.4774 bits (171), Expect = 3.467e-12
Identity = 39/113 (34.51%), Postives = 61/113 (53.98%), Query Frame = 1

Query:    1 DLANHFSEMAADY-HTDTP-HVLDYSKYPELEERQRFIRAYLSSEGK------KPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTL 315
            D+ NHF E   DY + + P +    + YP  E++  FIR YL+   K      + +  +   ++  + +Y LA+H FWGLW  + + ++ IEF Y EYA+ RFQ Y  +K  L
Sbjct:  276 DIGNHFCEWVYDYTYEEWPFYKARPADYPTREQQLLFIRHYLAEVQKGEVLSEEEQKKQEEDLLIEISRYALASHFFWGLWSTLQASMSTIEFGYLEYAQSRFQFYFQQKGQL 388

[Close]

BLAST of CD860256 vs. SwissProt
Match: EAS_DROME (Ethanolamine kinase OS=Drosophila melanogaster GN=eas PE=1 SV=2)

HSP 1 Score: 70.0922 bits (170), Expect = 4.528e-12
Identity = 36/108 (33.33%), Postives = 60/108 (55.56%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSS--EGKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLL 318
            D+ NHF+EM           +DYS+YP+ E + +++R YL    +    +N +V  +   V ++ LA+H+FW +W L+ +  + I+FDY  YA  R+ +Y  RK   L
Sbjct:  408 DIGNHFAEMCG------VDEVDYSRYPKREFQLQWLRVYLEEYLQRSNIQNDEVELLYVQVNQFALASHIFWTVWSLLQAEHSTIDFDYVGYAFLRYNEYLARKVEFL 509

[Close]

BLAST of CD860256 vs. SwissProt
Match: EKI1_MOUSE (Ethanolamine kinase 1 (Fragment) OS=Mus musculus GN=Etnk1 PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 7.724e-12
Identity = 38/104 (36.54%), Postives = 55/104 (52.88%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSE------GKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQY 294
            D+ NHF+E A          +DYS YP+ E + + +R+YL +       G      +V  +   V ++ LA+H FWGLW LI +  + IEFD+  YA  RF QY
Sbjct:  301 DIGNHFNEFAGVSD------VDYSLYPDRELQGQCVRSYLEAYKEYKGFGSDVTEKEVETLFIQVNQFALASHFFWGLWALIQAKYSTIEFDFLGYAVVRFNQY 398

[Close]

BLAST of CD860256 vs. SwissProt
Match: EKI2_HUMAN (Ethanolamine kinase 2 OS=Homo sapiens GN=ETNK2 PE=2 SV=3)

HSP 1 Score: 68.1662 bits (165), Expect = 1.721e-11
Identity = 35/99 (35.35%), Postives = 54/99 (54.55%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSE-GKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQY 294
            D+ NHF+E A        + +DY  YP  E + +++  YL ++ G      +V ++   V K+ LA+H FW LW LI +  + I+FD+  YA  RF QY
Sbjct:  280 DIGNHFNEFAG------VNEVDYCLYPARETQLQWLHYYLQAQKGMAVTPREVQRLYVQVNKFALASHFFWALWALIQNQYSTIDFDFLRYAVIRFNQY 372

[Close]

BLAST of CD860256 vs. TAIR peptide
Match: AT1G74320.1 (| Symbols: | Protein kinase superfamily protein | chr1:27941192-27942903 FORWARD LENGTH=350)

HSP 1 Score: 155.992 bits (393), Expect = 7.579e-39
Identity = 68/106 (64.15%), Postives = 87/106 (82.08%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSEGKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLL 318
            D+ANHF EMAADYHT+TPH++DYSKYP +EERQRF++ Y+S   +KP +  V +++  VEKYTLA+HL WGLWG+IS +VN+I+FDY EYARQRF+QY L K  LL
Sbjct:  239 DIANHFCEMAADYHTETPHIMDYSKYPGVEERQRFLKTYMSYSDEKPSDTMVKKLLEDVEKYTLASHLIWGLWGIISEHVNEIDFDYMEYARQRFEQYWLTKPRLL 344

[Close]

BLAST of CD860256 vs. TAIR peptide
Match: AT4G09760.1 (| Symbols: | Protein kinase superfamily protein | chr4:6148955-6151150 REVERSE LENGTH=346)

HSP 1 Score: 153.68 bits (387), Expect = 3.761e-38
Identity = 69/106 (65.09%), Postives = 87/106 (82.08%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSEGKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLL 318
            D+ANHF EMAADYH++TPH+LDY+ YP  EER+RFI  YL+S G++ +   + Q++  +EKYTLA+HLFWGLWG+IS YVNKIEFDY EY+RQRF+QY LRK  LL
Sbjct:  236 DIANHFCEMAADYHSNTPHILDYTLYPGEEERRRFICNYLTSSGEEAREEDIEQLLDDIEKYTLASHLFWGLWGIISGYVNKIEFDYIEYSRQRFKQYWLRKPKLL 341

[Close]

BLAST of CD860256 vs. TAIR peptide
Match: AT4G09760.2 (| Symbols: | Protein kinase superfamily protein | chr4:6148955-6151150 REVERSE LENGTH=346)

HSP 1 Score: 153.68 bits (387), Expect = 3.761e-38
Identity = 69/106 (65.09%), Postives = 87/106 (82.08%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSEGKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLL 318
            D+ANHF EMAADYH++TPH+LDY+ YP  EER+RFI  YL+S G++ +   + Q++  +EKYTLA+HLFWGLWG+IS YVNKIEFDY EY+RQRF+QY LRK  LL
Sbjct:  236 DIANHFCEMAADYHSNTPHILDYTLYPGEEERRRFICNYLTSSGEEAREEDIEQLLDDIEKYTLASHLFWGLWGIISGYVNKIEFDYIEYSRQRFKQYWLRKPKLL 341

[Close]

BLAST of CD860256 vs. TAIR peptide
Match: AT1G71697.1 (| Symbols: ATCK1, CK, CK1 | choline kinase 1 | chr1:26971537-26973177 FORWARD LENGTH=346)

HSP 1 Score: 149.443 bits (376), Expect = 7.093e-37
Identity = 68/107 (63.55%), Postives = 83/107 (77.57%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSEGKKPKNAKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRKSTLLD 321
            D+ANHF EMAA+YH+DTPHVLDY+ YP   ER+RFI  YL S G    + +V +++   E YTLANH+FWGLWG+IS +VNKIEFDY EYARQRF+QY LRK  LL+
Sbjct:  239 DIANHFCEMAANYHSDTPHVLDYTLYPGEGERRRFISTYLGSTGNATSDKEVERLLKDAESYTLANHIFWGLWGIISGHVNKIEFDYMEYARQRFEQYWLRKPLLLE 345

[Close]

BLAST of CD860256 vs. TAIR peptide
Match: AT2G26830.1 (| Symbols: emb1187 | Protein kinase superfamily protein | chr2:11443599-11446958 FORWARD LENGTH=374)

HSP 1 Score: 72.0182 bits (175), Expect = 1.439e-13
Identity = 38/105 (36.19%), Postives = 58/105 (55.24%), Query Frame = 1

Query:    1 DLANHFSEMAADYHTDTPHVLDYSKYPELEERQRFIRAYLSSEGKKPKN---AKVNQVVTAVEKYTLANHLFWGLWGLISSYVNKIEFDYKEYARQRFQQYRLRK 306
            D+ NHF+E A        +  DYS YP  EE+  FI+ YL  +  KP     A+V  V    + Y LA+HL+W +W +I + ++ IEF+Y  Y   R+ +Y+ +K
Sbjct:  264 DIGNHFNEYAG-------YDCDYSLYPSKEEQYHFIKHYLQPD--KPDEVSIAEVESVFVETDAYKLASHLYWAIWAIIQARMSPIEFEYLGYFFLRYNEYKKQK 359

The following BLAST results are available for this feature:

BLAST of CD860256 vs. TrEMBL
BLAST of CD860256 vs. SwissProt
BLAST of CD860256 vs. TAIR peptide

BLAST of CD860256 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q42810_SOYBN	2.035e-54	82.11	GmCK2p OS=Glycine max PE=2 SV=1	[more]
C6TJK8_SOYBN	6.547e-53	81.97	Putative uncharacterized protein OS=Glycine max PE...	[more]
Q42809_SOYBN	2.179e-48	75.83	GmCK1p OS=Glycine max PE=2 SV=1	[more]
B7FI98_MEDTR	8.571e-45	79.25	Putative uncharacterized protein OS=Medicago trunc...	[more]
Q9AVR9_PEA	9.476e-44	77.36	Choline kinase OS=Pisum sativum GN=ck1 PE=2 SV=1	[more]
A9PF05_POPTR	3.726e-40	66.67	Putative uncharacterized protein OS=Populus tricho...	[more]
Q42811_SOYBN	1.084e-39	58.59	GmCK3p (Fragment) OS=Glycine max PE=2 SV=1	[more]
B9SED6_RICCO	1.084e-39	61.16	Choline/ethanolamine kinase, putative OS=Ricinus c...	[more]
B9GX91_POPTR	3.154e-39	69.81	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
D7SXZ1_VITVI	4.120e-39	69.81	Whole genome shotgun sequence of line PN40024, sca...	[more]

BLAST of CD860256 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
EKI_NEMVE	2.565e-15	35.40	Probable ethanolamine kinase OS=Nematostella vecte...	[more]
EKI2_MOUSE	8.253e-14	38.38	Ethanolamine kinase 2 OS=Mus musculus GN=Etnk2 PE=...	[more]
CHKB_MOUSE	8.253e-14	33.90	Choline/ethanolamine kinase OS=Mus musculus GN=Chk...	[more]
CHKB_HUMAN	2.401e-13	36.28	Choline/ethanolamine kinase OS=Homo sapiens GN=CHK...	[more]
EKI1_HUMAN	1.556e-12	37.50	Ethanolamine kinase 1 OS=Homo sapiens GN=ETNK1 PE=...	[more]
EKI2_RAT	2.655e-12	36.36	Ethanolamine kinase 2 OS=Rattus norvegicus GN=Etnk...	[more]
CHKB_RAT	3.467e-12	34.51	Choline/ethanolamine kinase OS=Rattus norvegicus G...	[more]
EAS_DROME	4.528e-12	33.33	Ethanolamine kinase OS=Drosophila melanogaster GN=...	[more]
EKI1_MOUSE	7.724e-12	36.54	Ethanolamine kinase 1 (Fragment) OS=Mus musculus G...	[more]
EKI2_HUMAN	1.721e-11	35.35	Ethanolamine kinase 2 OS=Homo sapiens GN=ETNK2 PE=...	[more]

BLAST of CD860256 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT1G74320.1	7.579e-39	64.15	\| Symbols: \| Protein kinase superfamily protein \|...	[more]
AT4G09760.1	3.761e-38	65.09	\| Symbols: \| Protein kinase superfamily protein \|...	[more]
AT4G09760.2	3.761e-38	65.09	\| Symbols: \| Protein kinase superfamily protein \|...	[more]
AT1G71697.1	7.093e-37	63.55	\| Symbols: ATCK1, CK, CK1 \| choline kinase 1 \| chr...	[more]
AT2G26830.1	1.439e-13	36.19	\| Symbols: emb1187 \| Protein kinase superfamily pr...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002573	Choline/ethanolamine kinase	PANTHER	PTHR22603	CHOLINE/ETHANOALAMINE KINASE	coord: 1..115 score: 1.1
IPR002573	Choline/ethanolamine kinase	PFAM	PF01633	Choline_kinase	coord: 1..30 score: 1.2
IPR011009	Protein kinase-like domain	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 1..109 score: 2.2
None	No IPR available	GENE3D	3.90.1200.10	no description	coord: 1..105 score: 3.5
None	No IPR available	PANTHER	PTHR22603:SF7	CHOLINE/ETHANOLAMINE KINASE	coord: 1..115 score: 1.1

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD860256 ID=CD860256; Name=CD860256; organism=Pisum sativum; type=EST; length=439bp
GATCTCGCAAATCATTTCAGTGAAATGGCGGCCGATTACCATACCGATAC
ACCTCATGTTCTTGACTATAGTAAATATCCTGAACTCGAGGAGCGTCAAA
GGTTTATTCGCGCCTATCTTAGTTCTGAAGGTAAGAAACCGAAGAATGCT
AAAGTGAACCAAGTTGTGACTGCTGTGGAAAAATACACTCTTGCAAACCA
TCTATTTTGGGGCTTATGGGGACTTATTTCGAGTTATGTGAACAAAATTG
AGTTTGATTACAAGGAATATGCAAGGCAAAGGTTTCAGCAATACCGGTTA
AGGAAATCTACTTTATTGGACACTCCAACCATTGTTTCACAAAATGAAAC
TGTAAATGGTTCTCTACCACTAGCATCTTGTGTGTGATTTCTCTTATGCC
AAATTCCCATGTTTTAGTGAAGTTAATCTTAGTTTTTTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR002573	Choline/ethanolamine kinase
IPR011009	Kinase-like_dom_sf

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