GH720473

EST Overview
NameGH720473
Unique NameGH720473
TypeEST
OrganismPisum sativum (pea)
Sequence length584
Libraries
Library NameType
LIBEST_024271 552NIcdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v2Pisum sativum unigene v2EST
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of GH720473 vs. TrEMBL
Match: A2Q144_MEDTR (Protein NAP1 , putative OS=Medicago truncatula GN=MtrDRAFT_AC147481g25v2 PE=4 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 3.270e-83
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 1 
Query:  307 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLY 567
            EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLY
Sbjct:  362 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLY 448

HSP 2 Score: 157.147 bits (396), Expect = 3.270e-83
Identity = 81/99 (81.82%), Postives = 81/99 (81.82%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVGVASSKSKT RVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIA IRG         AG IRFLLGTP MVALDID SLKGLL QIVHHL  L K
Sbjct:  268 YFQHVGVASSKSKTTRVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAAIRGYSLSYLSSCAGRIRFLLGTPGMVALDIDASLKGLLQQIVHHLEHLPK 366
[Close]
BLAST of GH720473 vs. TrEMBL
Match: B7ZGK5_LOTJA (Nck-associated protein 1 OS=Lotus japonicus GN=nap PE=2 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 2.507e-80
Identity = 82/92 (89.13%), Postives = 87/92 (94.57%), Query Frame = 1 
Query:  307 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            E+LPKPQ EN+SAITCDLSDFRKDWLSILL+VTSSRSSINIRHLEKATVSTGKEGLLSEGN+AYNWSRCVDELES+LSKHGSLRKLY    H
Sbjct:  571 ENLPKPQGENVSAITCDLSDFRKDWLSILLIVTSSRSSINIRHLEKATVSTGKEGLLSEGNSAYNWSRCVDELESVLSKHGSLRKLYFYHQH 662

HSP 2 Score: 154.066 bits (388), Expect = 2.507e-80
Identity = 79/99 (79.80%), Postives = 80/99 (80.81%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            +FQHVGVASSKSKT RVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIA IRG         AG IRFLLGTP MVALDID  LKGLL QIVHHL  L K
Sbjct:  477 FFQHVGVASSKSKTTRVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAAIRGYSLSYLSSCAGRIRFLLGTPGMVALDIDAFLKGLLQQIVHHLENLPK 575
[Close]
BLAST of GH720473 vs. TrEMBL
Match: B7ZGK2_LOTJA (Nck-associated protein 1 OS=Lotus japonicus GN=nap PE=4 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 2.507e-80
Identity = 82/92 (89.13%), Postives = 87/92 (94.57%), Query Frame = 1 
Query:  307 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            E+LPKPQ EN+SAITCDLSDFRKDWLSILL+VTSSRSSINIRHLEKATVSTGKEGLLSEGN+AYNWSRCVDELES+LSKHGSLRKLY    H
Sbjct:  571 ENLPKPQGENVSAITCDLSDFRKDWLSILLIVTSSRSSINIRHLEKATVSTGKEGLLSEGNSAYNWSRCVDELESVLSKHGSLRKLYFYHQH 662

HSP 2 Score: 154.066 bits (388), Expect = 2.507e-80
Identity = 79/99 (79.80%), Postives = 80/99 (80.81%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            +FQHVGVASSKSKT RVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIA IRG         AG IRFLLGTP MVALDID  LKGLL QIVHHL  L K
Sbjct:  477 FFQHVGVASSKSKTTRVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAAIRGYSLSYLSSCAGRIRFLLGTPGMVALDIDAFLKGLLQQIVHHLENLPK 575
[Close]
BLAST of GH720473 vs. TrEMBL
Match: B9SCC0_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0890990 PE=4 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 2.105e-76
Identity = 78/92 (84.78%), Postives = 86/92 (93.48%), Query Frame = 1 
Query:  307 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            E++PKPQSEN+SAITCDLS+FRKDWLSIL++VTS+RSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELE  LS+HGSLRKLY    H
Sbjct:  568 ENIPKPQSENVSAITCDLSEFRKDWLSILMIVTSARSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELEFQLSRHGSLRKLYFYHQH 659

HSP 2 Score: 147.132 bits (370), Expect = 2.105e-76
Identity = 74/99 (74.75%), Postives = 79/99 (79.80%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVG+ASSKSK AR+VPVDIDPNDPTIGFLLDGMD LCCLVRKYIA IRG         AG IRFLLGTP M+ALD+D SLKGLL QIV HL  + K
Sbjct:  474 YFQHVGIASSKSKAARMVPVDIDPNDPTIGFLLDGMDRLCCLVRKYIAAIRGYALSYLSSCAGRIRFLLGTPGMLALDLDASLKGLLQQIVQHLENIPK 572
[Close]
BLAST of GH720473 vs. TrEMBL
Match: D7LHS3_ARALY (GRL/NAP1 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_902606 PE=4 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 5.810e-40
Identity = 82/103 (79.61%), Postives = 89/103 (86.41%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F R+    E++PK Q EN+SAITCDLSDFRKDWLSIL++VTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELES LSKHGSL+KLY    H
Sbjct:  584 KGLFQRIVQHLENIPKAQGENVSAITCDLSDFRKDWLSILMIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESQLSKHGSLKKLYFYHQH 686

HSP 2 Score: 138.658 bits (348), Expect = 2.890e-31
Identity = 66/99 (66.67%), Postives = 77/99 (77.78%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQH G+ASS+SK ARV+PVDIDPNDPTIGFLLDGMD LCCLVRKYI+  RG         AG IR+L+GTP +VALD+DP+LKGL  +IV HL  + K
Sbjct:  501 YFQHAGIASSRSKAARVIPVDIDPNDPTIGFLLDGMDRLCCLVRKYISAARGYALSYLSSSAGRIRYLMGTPGIVALDLDPTLKGLFQRIVQHLENIPK 599
[Close]
BLAST of GH720473 vs. TrEMBL
Match: Q0WMA5_ARATH (Putative uncharacterized protein At2g35110 OS=Arabidopsis thaliana GN=At2g35110 PE=2 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 9.910e-40
Identity = 82/103 (79.61%), Postives = 88/103 (85.44%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F R+    E +PK Q EN+SAITCDLSDFRKDWLSIL++VTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELES LSKHGSL+KLY    H
Sbjct:  146 KGLFQRIVQHLESIPKAQGENVSAITCDLSDFRKDWLSILMIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESQLSKHGSLKKLYFYHQH 248

HSP 2 Score: 139.043 bits (349), Expect = 2.213e-31
Identity = 66/99 (66.67%), Postives = 78/99 (78.79%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQH G+ASS+SK ARV+PVDIDPNDPTIGFLLDGMD LCCLVRKYI+  RG         AG IR+L+GTP +VALD+DP+LKGL  +IV HL ++ K
Sbjct:   63 YFQHAGIASSRSKAARVIPVDIDPNDPTIGFLLDGMDRLCCLVRKYISAARGYALSYLSSSAGRIRYLMGTPGIVALDLDPTLKGLFQRIVQHLESIPK 161
[Close]
BLAST of GH720473 vs. TrEMBL
Match: D7T9L3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_11.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00012103001 PE=4 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 6.424e-39
Identity = 80/103 (77.67%), Postives = 89/103 (86.41%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F ++    E++PKPQ ENISAITC+LS+ RKDWLSIL++VTS+RSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELES LSKHGSLRKLY    H
Sbjct:  563 KGLFQKIVQHLENIPKPQGENISAITCNLSELRKDWLSILMIVTSARSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESQLSKHGSLRKLYFYHQH 665

HSP 2 Score: 147.132 bits (370), Expect = 8.126e-34
Identity = 73/99 (73.74%), Postives = 80/99 (80.81%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVG+ASSKSKTAR+VPVDIDP+DPTIGFLLDGMDHLCCLVRKYIA IRG         AG IRFLLGTP MVALD+D +LKGL  +IV HL  + K
Sbjct:  480 YFQHVGIASSKSKTARMVPVDIDPSDPTIGFLLDGMDHLCCLVRKYIAAIRGYALSFLSSCAGRIRFLLGTPGMVALDLDANLKGLFQKIVQHLENIPK 578
[Close]
BLAST of GH720473 vs. TrEMBL
Match: Q0J3Z5_ORYSJ (Os08g0544500 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os08g0544500 PE=4 SV=2)

HSP 1 Score: 157.532 bits (397), Expect = 6.012e-37
Identity = 75/103 (72.82%), Postives = 86/103 (83.50%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F ++    E++PKPQ EN+ AITCDL+D RK WLSIL++VTSSRSS+NIRHLEKATVSTGKEGL+SEGNAAYNWSRCVDELE  LSKHGSL+KLY    H
Sbjct:  546 KGLFQQVLHCLENIPKPQGENVPAITCDLTDLRKHWLSILMIVTSSRSSVNIRHLEKATVSTGKEGLVSEGNAAYNWSRCVDELEGQLSKHGSLKKLYFYHQH 648

HSP 2 Score: 119.013 bits (297), Expect = 2.370e-25
Identity = 63/99 (63.64%), Postives = 70/99 (70.71%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVG+ASSKS   R V  DID  DPTIGFLLDGM  LCCLVRKYIA I+G         AG IRFLLGTP MVALD+D +LKGL  Q++H L  + K
Sbjct:  465 YFQHVGIASSKSSRGRTV--DIDAADPTIGFLLDGMGKLCCLVRKYIAAIKGYALSYLSSCAGRIRFLLGTPGMVALDLDATLKGLFQQVLHCLENIPK 561
[Close]
BLAST of GH720473 vs. TrEMBL
Match: B9ICQ5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_575639 PE=4 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 6.012e-37
Identity = 77/103 (74.76%), Postives = 87/103 (84.47%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F ++    E +PK Q ENISAI CDLS+FRKDWLSIL++VTS+RSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVD+LES LSKHG+L+KLY    H
Sbjct:  389 KGLFQQIVQHLESIPKLQGENISAIMCDLSEFRKDWLSILMIVTSARSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDDLESQLSKHGTLKKLYFYHQH 491

HSP 2 Score: 149.443 bits (376), Expect = 1.637e-34
Identity = 74/99 (74.75%), Postives = 79/99 (79.80%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVG+ASSKSK AR +PVDIDPNDPTIGFLLDGMDHLCCLVRKYIA IRG         AG IRFLLGTP MVALD+D SLKGL  QIV HL ++ K
Sbjct:  306 YFQHVGIASSKSKAARAIPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAAIRGYALSYLSSCAGRIRFLLGTPGMVALDLDASLKGLFQQIVQHLESIPK 404
[Close]
BLAST of GH720473 vs. TrEMBL
Match: B9FYE3_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_28157 PE=4 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 6.012e-37
Identity = 75/103 (72.82%), Postives = 86/103 (83.50%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F ++    E++PKPQ EN+ AITCDL+D RK WLSIL++VTSSRSS+NIRHLEKATVSTGKEGL+SEGNAAYNWSRCVDELE  LSKHGSL+KLY    H
Sbjct:  540 KGLFQQVLHCLENIPKPQGENVPAITCDLTDLRKHWLSILMIVTSSRSSVNIRHLEKATVSTGKEGLVSEGNAAYNWSRCVDELEGQLSKHGSLKKLYFYHQH 642

HSP 2 Score: 119.013 bits (297), Expect = 2.370e-25
Identity = 63/99 (63.64%), Postives = 70/99 (70.71%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVG+ASSKS   R V  DID  DPTIGFLLDGM  LCCLVRKYIA I+G         AG IRFLLGTP MVALD+D +LKGL  Q++H L  + K
Sbjct:  459 YFQHVGIASSKSSRGRTV--DIDAADPTIGFLLDGMGKLCCLVRKYIAAIKGYALSYLSSCAGRIRFLLGTPGMVALDLDATLKGLFQQVLHCLENIPK 555
[Close]
BLAST of GH720473 vs. SwissProt
Match: NCKP1_ARATH (Protein NAP1 OS=Arabidopsis thaliana GN=NAP1 PE=1 SV=2)

HSP 1 Score: 166.777 bits (421), Expect = 7.227e-41
Identity = 82/103 (79.61%), Postives = 88/103 (85.44%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F R+    E +PK Q EN+SAITCDLSDFRKDWLSIL++VTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELES LSKHGSL+KLY    H
Sbjct:  582 KGLFQRIVQHLESIPKAQGENVSAITCDLSDFRKDWLSILMIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESQLSKHGSLKKLYFYHQH 684

HSP 2 Score: 139.043 bits (349), Expect = 1.614e-32
Identity = 66/99 (66.67%), Postives = 78/99 (78.79%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQH G+ASS+SK ARV+PVDIDPNDPTIGFLLDGMD LCCLVRKYI+  RG         AG IR+L+GTP +VALD+DP+LKGL  +IV HL ++ K
Sbjct:  499 YFQHAGIASSRSKAARVIPVDIDPNDPTIGFLLDGMDRLCCLVRKYISAARGYALSYLSSSAGRIRYLMGTPGIVALDLDPTLKGLFQRIVQHLESIPK 597
[Close]
BLAST of GH720473 vs. SwissProt
Match: NCKP1_ORYSJ (Probable protein NAP1 OS=Oryza sativa subsp. japonica GN=NAP1 PE=2 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 4.384e-38
Identity = 75/103 (72.82%), Postives = 86/103 (83.50%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F ++    E++PKPQ EN+ AITCDL+D RK WLSIL++VTSSRSS+NIRHLEKATVSTGKEGL+SEGNAAYNWSRCVDELE  LSKHGSL+KLY    H
Sbjct:  540 KGLFQQVLHCLENIPKPQGENVPAITCDLTDLRKHWLSILMIVTSSRSSVNIRHLEKATVSTGKEGLVSEGNAAYNWSRCVDELEGQLSKHGSLKKLYFYHQH 642

HSP 2 Score: 119.013 bits (297), Expect = 1.728e-26
Identity = 63/99 (63.64%), Postives = 70/99 (70.71%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVG+ASSKS   R V  DID  DPTIGFLLDGM  LCCLVRKYIA I+G         AG IRFLLGTP MVALD+D +LKGL  Q++H L  + K
Sbjct:  459 YFQHVGIASSKSSRGRTV--DIDAADPTIGFLLDGMGKLCCLVRKYIAAIKGYALSYLSSCAGRIRFLLGTPGMVALDLDATLKGLFQQVLHCLENIPK 555
[Close]
BLAST of GH720473 vs. TAIR peptide
Match: AT2G35110.2 (| Symbols: GRL, NAP1, NAPP | transcription activators | chr2:14795970-14803525 REVERSE LENGTH=1396)

HSP 1 Score: 166.777 bits (421), Expect = 7.631e-42
Identity = 82/103 (79.61%), Postives = 88/103 (85.44%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F R+    E +PK Q EN+SAITCDLSDFRKDWLSIL++VTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELES LSKHGSL+KLY    H
Sbjct:  553 KGLFQRIVQHLESIPKAQGENVSAITCDLSDFRKDWLSILMIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESQLSKHGSLKKLYFYHQH 655

HSP 2 Score: 139.043 bits (349), Expect = 1.704e-33
Identity = 66/99 (66.67%), Postives = 78/99 (78.79%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQH G+ASS+SK ARV+PVDIDPNDPTIGFLLDGMD LCCLVRKYI+  RG         AG IR+L+GTP +VALD+DP+LKGL  +IV HL ++ K
Sbjct:  470 YFQHAGIASSRSKAARVIPVDIDPNDPTIGFLLDGMDRLCCLVRKYISAARGYALSYLSSSAGRIRYLMGTPGIVALDLDPTLKGLFQRIVQHLESIPK 568
[Close]
BLAST of GH720473 vs. TAIR peptide
Match: AT2G35110.1 (| Symbols: GRL, NAP1, NAPP | transcription activators | chr2:14795970-14803525 REVERSE LENGTH=1425)

HSP 1 Score: 166.777 bits (421), Expect = 7.631e-42
Identity = 82/103 (79.61%), Postives = 88/103 (85.44%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F R+    E +PK Q EN+SAITCDLSDFRKDWLSIL++VTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELES LSKHGSL+KLY    H
Sbjct:  582 KGLFQRIVQHLESIPKAQGENVSAITCDLSDFRKDWLSILMIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESQLSKHGSLKKLYFYHQH 684

HSP 2 Score: 139.043 bits (349), Expect = 1.704e-33
Identity = 66/99 (66.67%), Postives = 78/99 (78.79%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQH G+ASS+SK ARV+PVDIDPNDPTIGFLLDGMD LCCLVRKYI+  RG         AG IR+L+GTP +VALD+DP+LKGL  +IV HL ++ K
Sbjct:  499 YFQHAGIASSRSKAARVIPVDIDPNDPTIGFLLDGMDRLCCLVRKYISAARGYALSYLSSSAGRIRYLMGTPGIVALDLDPTLKGLFQRIVQHLESIPK 597
[Close]
BLAST of GH720473 vs. TAIR peptide
Match: AT2G35110.2 (| Symbols: GRL, NAP1, NAPP | transcription activators | chr2:14795970-14803525 REVERSE LENGTH=1396)

HSP 1 Score: 166.777 bits (421), Expect = 7.631e-42
Identity = 82/103 (79.61%), Postives = 88/103 (85.44%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F R+    E +PK Q EN+SAITCDLSDFRKDWLSIL++VTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELES LSKHGSL+KLY    H
Sbjct:  553 KGLFQRIVQHLESIPKAQGENVSAITCDLSDFRKDWLSILMIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESQLSKHGSLKKLYFYHQH 655

HSP 2 Score: 139.043 bits (349), Expect = 1.704e-33
Identity = 66/99 (66.67%), Postives = 78/99 (78.79%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQH G+ASS+SK ARV+PVDIDPNDPTIGFLLDGMD LCCLVRKYI+  RG         AG IR+L+GTP +VALD+DP+LKGL  +IV HL ++ K
Sbjct:  470 YFQHAGIASSRSKAARVIPVDIDPNDPTIGFLLDGMDRLCCLVRKYISAARGYALSYLSSSAGRIRYLMGTPGIVALDLDPTLKGLFQRIVQHLESIPK 568
[Close]
BLAST of GH720473 vs. TAIR peptide
Match: AT2G35110.1 (| Symbols: GRL, NAP1, NAPP | transcription activators | chr2:14795970-14803525 REVERSE LENGTH=1425)

HSP 1 Score: 166.777 bits (421), Expect = 7.631e-42
Identity = 82/103 (79.61%), Postives = 88/103 (85.44%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F R+    E +PK Q EN+SAITCDLSDFRKDWLSIL++VTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELES LSKHGSL+KLY    H
Sbjct:  582 KGLFQRIVQHLESIPKAQGENVSAITCDLSDFRKDWLSILMIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESQLSKHGSLKKLYFYHQH 684

HSP 2 Score: 139.043 bits (349), Expect = 1.704e-33
Identity = 66/99 (66.67%), Postives = 78/99 (78.79%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQH G+ASS+SK ARV+PVDIDPNDPTIGFLLDGMD LCCLVRKYI+  RG         AG IR+L+GTP +VALD+DP+LKGL  +IV HL ++ K
Sbjct:  499 YFQHAGIASSRSKAARVIPVDIDPNDPTIGFLLDGMDRLCCLVRKYISAARGYALSYLSSSAGRIRYLMGTPGIVALDLDPTLKGLFQRIVQHLESIPK 597
[Close]
BLAST of GH720473 vs. TrEMBL
Match: E3UV65_MEDTR (NAP1 OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 4.001e-83
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 1 
Query:  307 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLY 567
            EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLY
Sbjct:  572 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLY 658

HSP 2 Score: 157.147 bits (396), Expect = 4.001e-83
Identity = 81/99 (81.82%), Postives = 81/99 (81.82%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVGVASSKSKT RVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIA IRG         AG IRFLLGTP MVALDID SLKGLL QIVHHL  L K
Sbjct:  478 YFQHVGVASSKSKTTRVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAAIRGYSLSYLSSCAGRIRFLLGTPGMVALDIDASLKGLLQQIVHHLEHLPK 576
[Close]
BLAST of GH720473 vs. TrEMBL
Match: A2Q144_MEDTR (Protein NAP1 , putative OS=Medicago truncatula GN=MtrDRAFT_AC147481g25v2 PE=4 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 4.004e-83
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 1 
Query:  307 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLY 567
            EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLY
Sbjct:  362 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLY 448

HSP 2 Score: 157.147 bits (396), Expect = 4.004e-83
Identity = 81/99 (81.82%), Postives = 81/99 (81.82%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVGVASSKSKT RVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIA IRG         AG IRFLLGTP MVALDID SLKGLL QIVHHL  L K
Sbjct:  268 YFQHVGVASSKSKTTRVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAAIRGYSLSYLSSCAGRIRFLLGTPGMVALDIDASLKGLLQQIVHHLEHLPK 366
[Close]
BLAST of GH720473 vs. TrEMBL
Match: B7ZGK5_LOTJA (Nck-associated protein 1 OS=Lotus japonicus GN=nap PE=2 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 3.070e-80
Identity = 82/92 (89.13%), Postives = 87/92 (94.57%), Query Frame = 1 
Query:  307 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            E+LPKPQ EN+SAITCDLSDFRKDWLSILL+VTSSRSSINIRHLEKATVSTGKEGLLSEGN+AYNWSRCVDELES+LSKHGSLRKLY    H
Sbjct:  571 ENLPKPQGENVSAITCDLSDFRKDWLSILLIVTSSRSSINIRHLEKATVSTGKEGLLSEGNSAYNWSRCVDELESVLSKHGSLRKLYFYHQH 662

HSP 2 Score: 154.066 bits (388), Expect = 3.070e-80
Identity = 79/99 (79.80%), Postives = 80/99 (80.81%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            +FQHVGVASSKSKT RVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIA IRG         AG IRFLLGTP MVALDID  LKGLL QIVHHL  L K
Sbjct:  477 FFQHVGVASSKSKTTRVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAAIRGYSLSYLSSCAGRIRFLLGTPGMVALDIDAFLKGLLQQIVHHLENLPK 575
[Close]
BLAST of GH720473 vs. TrEMBL
Match: B7ZGK2_LOTJA (Nck-associated protein 1 OS=Lotus japonicus GN=nap PE=4 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 3.070e-80
Identity = 82/92 (89.13%), Postives = 87/92 (94.57%), Query Frame = 1 
Query:  307 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            E+LPKPQ EN+SAITCDLSDFRKDWLSILL+VTSSRSSINIRHLEKATVSTGKEGLLSEGN+AYNWSRCVDELES+LSKHGSLRKLY    H
Sbjct:  571 ENLPKPQGENVSAITCDLSDFRKDWLSILLIVTSSRSSINIRHLEKATVSTGKEGLLSEGNSAYNWSRCVDELESVLSKHGSLRKLYFYHQH 662

HSP 2 Score: 154.066 bits (388), Expect = 3.070e-80
Identity = 79/99 (79.80%), Postives = 80/99 (80.81%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            +FQHVGVASSKSKT RVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIA IRG         AG IRFLLGTP MVALDID  LKGLL QIVHHL  L K
Sbjct:  477 FFQHVGVASSKSKTTRVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAAIRGYSLSYLSSCAGRIRFLLGTPGMVALDIDAFLKGLLQQIVHHLENLPK 575
[Close]
BLAST of GH720473 vs. TrEMBL
Match: B9SCC0_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0890990 PE=4 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 2.578e-76
Identity = 78/92 (84.78%), Postives = 86/92 (93.48%), Query Frame = 1 
Query:  307 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            E++PKPQSEN+SAITCDLS+FRKDWLSIL++VTS+RSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELE  LS+HGSLRKLY    H
Sbjct:  568 ENIPKPQSENVSAITCDLSEFRKDWLSILMIVTSARSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELEFQLSRHGSLRKLYFYHQH 659

HSP 2 Score: 147.132 bits (370), Expect = 2.578e-76
Identity = 74/99 (74.75%), Postives = 79/99 (79.80%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVG+ASSKSK AR+VPVDIDPNDPTIGFLLDGMD LCCLVRKYIA IRG         AG IRFLLGTP M+ALD+D SLKGLL QIV HL  + K
Sbjct:  474 YFQHVGIASSKSKAARMVPVDIDPNDPTIGFLLDGMDRLCCLVRKYIAAIRGYALSYLSSCAGRIRFLLGTPGMLALDLDASLKGLLQQIVQHLENIPK 572
[Close]
BLAST of GH720473 vs. TrEMBL
Match: D7LHS3_ARALY (GRL/NAP1 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_902606 PE=4 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 7.114e-40
Identity = 82/103 (79.61%), Postives = 89/103 (86.41%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F R+    E++PK Q EN+SAITCDLSDFRKDWLSIL++VTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELES LSKHGSL+KLY    H
Sbjct:  584 KGLFQRIVQHLENIPKAQGENVSAITCDLSDFRKDWLSILMIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESQLSKHGSLKKLYFYHQH 686

HSP 2 Score: 138.658 bits (348), Expect = 3.539e-31
Identity = 66/99 (66.67%), Postives = 77/99 (77.78%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQH G+ASS+SK ARV+PVDIDPNDPTIGFLLDGMD LCCLVRKYI+  RG         AG IR+L+GTP +VALD+DP+LKGL  +IV HL  + K
Sbjct:  501 YFQHAGIASSRSKAARVIPVDIDPNDPTIGFLLDGMDRLCCLVRKYISAARGYALSYLSSSAGRIRYLMGTPGIVALDLDPTLKGLFQRIVQHLENIPK 599
[Close]
BLAST of GH720473 vs. TrEMBL
Match: Q0WMA5_ARATH (Putative uncharacterized protein At2g35110 OS=Arabidopsis thaliana GN=At2g35110 PE=2 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 1.213e-39
Identity = 82/103 (79.61%), Postives = 88/103 (85.44%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F R+    E +PK Q EN+SAITCDLSDFRKDWLSIL++VTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELES LSKHGSL+KLY    H
Sbjct:  146 KGLFQRIVQHLESIPKAQGENVSAITCDLSDFRKDWLSILMIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESQLSKHGSLKKLYFYHQH 248

HSP 2 Score: 139.043 bits (349), Expect = 2.710e-31
Identity = 66/99 (66.67%), Postives = 78/99 (78.79%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQH G+ASS+SK ARV+PVDIDPNDPTIGFLLDGMD LCCLVRKYI+  RG         AG IR+L+GTP +VALD+DP+LKGL  +IV HL ++ K
Sbjct:   63 YFQHAGIASSRSKAARVIPVDIDPNDPTIGFLLDGMDRLCCLVRKYISAARGYALSYLSSSAGRIRYLMGTPGIVALDLDPTLKGLFQRIVQHLESIPK 161
[Close]
BLAST of GH720473 vs. TrEMBL
Match: D7T9L3_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_11.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00012103001 PE=4 SV=1)

HSP 1 Score: 164.081 bits (414), Expect = 7.865e-39
Identity = 80/103 (77.67%), Postives = 89/103 (86.41%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F ++    E++PKPQ ENISAITC+LS+ RKDWLSIL++VTS+RSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELES LSKHGSLRKLY    H
Sbjct:  563 KGLFQKIVQHLENIPKPQGENISAITCNLSELRKDWLSILMIVTSARSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESQLSKHGSLRKLYFYHQH 665

HSP 2 Score: 147.132 bits (370), Expect = 9.950e-34
Identity = 73/99 (73.74%), Postives = 80/99 (80.81%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVG+ASSKSKTAR+VPVDIDP+DPTIGFLLDGMDHLCCLVRKYIA IRG         AG IRFLLGTP MVALD+D +LKGL  +IV HL  + K
Sbjct:  480 YFQHVGIASSKSKTARMVPVDIDPSDPTIGFLLDGMDHLCCLVRKYIAAIRGYALSFLSSCAGRIRFLLGTPGMVALDLDANLKGLFQKIVQHLENIPK 578
[Close]
BLAST of GH720473 vs. TrEMBL
Match: Q0J3Z5_ORYSJ (Os08g0544500 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os08g0544500 PE=4 SV=2)

HSP 1 Score: 157.532 bits (397), Expect = 7.362e-37
Identity = 75/103 (72.82%), Postives = 86/103 (83.50%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F ++    E++PKPQ EN+ AITCDL+D RK WLSIL++VTSSRSS+NIRHLEKATVSTGKEGL+SEGNAAYNWSRCVDELE  LSKHGSL+KLY    H
Sbjct:  546 KGLFQQVLHCLENIPKPQGENVPAITCDLTDLRKHWLSILMIVTSSRSSVNIRHLEKATVSTGKEGLVSEGNAAYNWSRCVDELEGQLSKHGSLKKLYFYHQH 648

HSP 2 Score: 119.013 bits (297), Expect = 2.902e-25
Identity = 63/99 (63.64%), Postives = 70/99 (70.71%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVG+ASSKS   R V  DID  DPTIGFLLDGM  LCCLVRKYIA I+G         AG IRFLLGTP MVALD+D +LKGL  Q++H L  + K
Sbjct:  465 YFQHVGIASSKSSRGRTV--DIDAADPTIGFLLDGMGKLCCLVRKYIAAIKGYALSYLSSCAGRIRFLLGTPGMVALDLDATLKGLFQQVLHCLENIPK 561
[Close]
BLAST of GH720473 vs. TrEMBL
Match: B9ICQ5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_575639 PE=4 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 7.362e-37
Identity = 77/103 (74.76%), Postives = 87/103 (84.47%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F ++    E +PK Q ENISAI CDLS+FRKDWLSIL++VTS+RSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVD+LES LSKHG+L+KLY    H
Sbjct:  389 KGLFQQIVQHLESIPKLQGENISAIMCDLSEFRKDWLSILMIVTSARSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDDLESQLSKHGTLKKLYFYHQH 491

HSP 2 Score: 149.443 bits (376), Expect = 2.005e-34
Identity = 74/99 (74.75%), Postives = 79/99 (79.80%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVG+ASSKSK AR +PVDIDPNDPTIGFLLDGMDHLCCLVRKYIA IRG         AG IRFLLGTP MVALD+D SLKGL  QIV HL ++ K
Sbjct:  306 YFQHVGIASSKSKAARAIPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAAIRGYALSYLSSCAGRIRFLLGTPGMVALDLDASLKGLFQQIVQHLESIPK 404
[Close]
BLAST of GH720473 vs. Lotus protein
Match: chr4.CM2115.90.r2.m (+ phase: 0 )

HSP 1 Score: 169.859 bits (429), Expect = 1.140e-82
Identity = 82/92 (89.13%), Postives = 87/92 (94.57%), Query Frame = 1 
Query:  307 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            E+LPKPQ EN+SAITCDLSDFRKDWLSILL+VTSSRSSINIRHLEKATVSTGKEGLLSEGN+AYNWSRCVDELES+LSKHGSLRKLY    H
Sbjct:  608 ENLPKPQGENVSAITCDLSDFRKDWLSILLIVTSSRSSINIRHLEKATVSTGKEGLLSEGNSAYNWSRCVDELESVLSKHGSLRKLYFYHQH 699

HSP 2 Score: 154.066 bits (388), Expect = 1.140e-82
Identity = 79/99 (79.80%), Postives = 80/99 (80.81%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            +FQHVGVASSKSKT RVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIA IRG         AG IRFLLGTP MVALDID  LKGLL QIVHHL  L K
Sbjct:  514 FFQHVGVASSKSKTTRVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAAIRGYSLSYLSSCAGRIRFLLGTPGMVALDIDAFLKGLLQQIVHHLENLPK 612
[Close]
BLAST of GH720473 vs. Soybean peptides
Match: Glyma20g02370.1|PACid:16315257 ()

HSP 1 Score: 169.474 bits (428), Expect = 2.220e-82
Identity = 82/92 (89.13%), Postives = 87/92 (94.57%), Query Frame = 1 
Query:  307 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            E+LPKPQ ENISAITCD+SDFRKDWLSILL+VTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELES+LSKHGSLR+LY    H
Sbjct:  675 ENLPKPQGENISAITCDMSDFRKDWLSILLIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESVLSKHGSLRRLYFYHQH 766

HSP 2 Score: 154.836 bits (390), Expect = 2.220e-82
Identity = 78/99 (78.79%), Postives = 81/99 (81.82%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVG+ASS+SKT RVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIA IRG         AG IRFLLGTP MVALDI+ SLKGLL QIVHHL  L K
Sbjct:  581 YFQHVGIASSRSKTTRVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAAIRGYSLSYLSSCAGRIRFLLGTPGMVALDIEASLKGLLQQIVHHLENLPK 679
[Close]
BLAST of GH720473 vs. Soybean peptides
Match: Glyma20g02370.2|PACid:16315258 ()

HSP 1 Score: 169.474 bits (428), Expect = 2.222e-82
Identity = 82/92 (89.13%), Postives = 87/92 (94.57%), Query Frame = 1 
Query:  307 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            E+LPKPQ ENISAITCD+SDFRKDWLSILL+VTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELES+LSKHGSLR+LY    H
Sbjct:  675 ENLPKPQGENISAITCDMSDFRKDWLSILLIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESVLSKHGSLRRLYFYHQH 766

HSP 2 Score: 154.836 bits (390), Expect = 2.222e-82
Identity = 78/99 (78.79%), Postives = 81/99 (81.82%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVG+ASS+SKT RVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIA IRG         AG IRFLLGTP MVALDI+ SLKGLL QIVHHL  L K
Sbjct:  581 YFQHVGIASSRSKTTRVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAAIRGYSLSYLSSCAGRIRFLLGTPGMVALDIEASLKGLLQQIVHHLENLPK 679
[Close]
BLAST of GH720473 vs. Soybean peptides
Match: Glyma20g02370.3|PACid:16315259 ()

HSP 1 Score: 130.568 bits (327), Expect = 5.266e-63
Identity = 63/67 (94.03%), Postives = 66/67 (98.51%), Query Frame = 1 
Query:  307 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWS 507
            E+LPKPQ ENISAITCD+SDFRKDWLSILL+VTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWS
Sbjct:  521 ENLPKPQGENISAITCDMSDFRKDWLSILLIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWS 587

HSP 2 Score: 129.028 bits (323), Expect = 5.266e-63
Identity = 68/99 (68.69%), Postives = 71/99 (71.72%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVG+ASS+SKT RVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIA                     GTP MVALDI+ SLKGLL QIVHHL  L K
Sbjct:  448 YFQHVGIASSRSKTTRVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIA---------------------GTPGMVALDIEASLKGLLQQIVHHLENLPK 525
[Close]
BLAST of GH720473 vs. SwissProt
Match: NCKP1_ARATH (Protein NAP1 OS=Arabidopsis thaliana GN=NAP1 PE=1 SV=2)

HSP 1 Score: 166.777 bits (421), Expect = 7.358e-41
Identity = 82/103 (79.61%), Postives = 88/103 (85.44%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F R+    E +PK Q EN+SAITCDLSDFRKDWLSIL++VTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELES LSKHGSL+KLY    H
Sbjct:  582 KGLFQRIVQHLESIPKAQGENVSAITCDLSDFRKDWLSILMIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESQLSKHGSLKKLYFYHQH 684

HSP 2 Score: 139.043 bits (349), Expect = 1.643e-32
Identity = 66/99 (66.67%), Postives = 78/99 (78.79%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQH G+ASS+SK ARV+PVDIDPNDPTIGFLLDGMD LCCLVRKYI+  RG         AG IR+L+GTP +VALD+DP+LKGL  +IV HL ++ K
Sbjct:  499 YFQHAGIASSRSKAARVIPVDIDPNDPTIGFLLDGMDRLCCLVRKYISAARGYALSYLSSSAGRIRYLMGTPGIVALDLDPTLKGLFQRIVQHLESIPK 597
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BLAST of GH720473 vs. SwissProt
Match: NCKP1_ORYSJ (Probable protein NAP1 OS=Oryza sativa subsp. japonica GN=NAP1 PE=2 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 4.464e-38
Identity = 75/103 (72.82%), Postives = 86/103 (83.50%), Query Frame = 1 
Query:  274 KDFFTRLSTTSEHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLYLGRDH 582
            K  F ++    E++PKPQ EN+ AITCDL+D RK WLSIL++VTSSRSS+NIRHLEKATVSTGKEGL+SEGNAAYNWSRCVDELE  LSKHGSL+KLY    H
Sbjct:  540 KGLFQQVLHCLENIPKPQGENVPAITCDLTDLRKHWLSILMIVTSSRSSVNIRHLEKATVSTGKEGLVSEGNAAYNWSRCVDELEGQLSKHGSLKKLYFYHQH 642

HSP 2 Score: 119.013 bits (297), Expect = 1.760e-26
Identity = 63/99 (63.64%), Postives = 70/99 (70.71%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVG+ASSKS   R V  DID  DPTIGFLLDGM  LCCLVRKYIA I+G         AG IRFLLGTP MVALD+D +LKGL  Q++H L  + K
Sbjct:  459 YFQHVGIASSKSSRGRTV--DIDAADPTIGFLLDGMGKLCCLVRKYIAAIKGYALSYLSSCAGRIRFLLGTPGMVALDLDATLKGLFQQVLHCLENIPK 555
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BLAST of GH720473 vs. Medicago proteins
Match: IMGA|Medtr4g084140.1 (Nck-associated protein 1 (AHRD V1 **-- B7ZGK5_LOTJA) chr04_pseudomolecule_IMGAG_V3.5 28873693-28890115 E EGN_Mt100125 20100825)

HSP 1 Score: 176.407 bits (446), Expect = 2.183e-85
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 1 
Query:  307 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLY 567
            EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLY
Sbjct:  625 EHLPKPQSENISAITCDLSDFRKDWLSILLMVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESLLSKHGSLRKLY 711

HSP 2 Score: 157.147 bits (396), Expect = 2.183e-85
Identity = 81/99 (81.82%), Postives = 81/99 (81.82%), Query Frame = 3 
Query:   24 YFQHVGVASSKSKTARVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAPIRGXXXXXXXXXAGIIRFLLGTPVMVALDIDPSLKGLLHQIVHHL*TLAK 320
            YFQHVGVASSKSKT RVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIA IRG         AG IRFLLGTP MVALDID SLKGLL QIVHHL  L K
Sbjct:  531 YFQHVGVASSKSKTTRVVPVDIDPNDPTIGFLLDGMDHLCCLVRKYIAAIRGYSLSYLSSCAGRIRFLLGTPGMVALDIDASLKGLLQQIVHHLEHLPK 629
The following BLAST results are available for this feature:
BLAST of GH720473 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
A2Q144_MEDTR3.270e-83100.00Protein NAP1 , putative OS=Medicago truncatula GN=... [more]
B7ZGK5_LOTJA2.507e-8089.13Nck-associated protein 1 OS=Lotus japonicus GN=nap... [more]
B7ZGK2_LOTJA2.507e-8089.13Nck-associated protein 1 OS=Lotus japonicus GN=nap... [more]
B9SCC0_RICCO2.105e-7684.78Putative uncharacterized protein OS=Ricinus commun... [more]
D7LHS3_ARALY5.810e-4079.61GRL/NAP1 OS=Arabidopsis lyrata subsp. lyrata GN=AR... [more]
Q0WMA5_ARATH9.910e-4079.61Putative uncharacterized protein At2g35110 OS=Arab... [more]
D7T9L3_VITVI6.424e-3977.67Whole genome shotgun sequence of line PN40024, sca... [more]
Q0J3Z5_ORYSJ6.012e-3772.82Os08g0544500 protein (Fragment) OS=Oryza sativa su... [more]
B9ICQ5_POPTR6.012e-3774.76Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9FYE3_ORYSJ6.012e-3772.82Putative uncharacterized protein OS=Oryza sativa s... [more]
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BLAST of GH720473 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 2
Match NameE-valueIdentityDescription
NCKP1_ARATH7.227e-4179.61Protein NAP1 OS=Arabidopsis thaliana GN=NAP1 PE=1 ... [more]
NCKP1_ORYSJ4.384e-3872.82Probable protein NAP1 OS=Oryza sativa subsp. japon... [more]
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BLAST of GH720473 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT2G35110.27.631e-4279.61| Symbols: GRL, NAP1, NAPP | transcription activat... [more]
AT2G35110.17.631e-4279.61| Symbols: GRL, NAP1, NAPP | transcription activat... [more]
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BLAST of GH720473 vs. TAIR peptide
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT2G35110.27.631e-4279.61| Symbols: GRL, NAP1, NAPP | transcription activat... [more]
AT2G35110.17.631e-4279.61| Symbols: GRL, NAP1, NAPP | transcription activat... [more]
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BLAST of GH720473 vs. TrEMBL
Analysis Date: 2011-04-27 (Homology Analysis: Pisum sativum unigene v2 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
E3UV65_MEDTR4.001e-83100.00NAP1 OS=Medicago truncatula PE=2 SV=1[more]
A2Q144_MEDTR4.004e-83100.00Protein NAP1 , putative OS=Medicago truncatula GN=... [more]
B7ZGK5_LOTJA3.070e-8089.13Nck-associated protein 1 OS=Lotus japonicus GN=nap... [more]
B7ZGK2_LOTJA3.070e-8089.13Nck-associated protein 1 OS=Lotus japonicus GN=nap... [more]
B9SCC0_RICCO2.578e-7684.78Putative uncharacterized protein OS=Ricinus commun... [more]
D7LHS3_ARALY7.114e-4079.61GRL/NAP1 OS=Arabidopsis lyrata subsp. lyrata GN=AR... [more]
Q0WMA5_ARATH1.213e-3979.61Putative uncharacterized protein At2g35110 OS=Arab... [more]
D7T9L3_VITVI7.865e-3977.67Whole genome shotgun sequence of line PN40024, sca... [more]
Q0J3Z5_ORYSJ7.362e-3772.82Os08g0544500 protein (Fragment) OS=Oryza sativa su... [more]
B9ICQ5_POPTR7.362e-3774.76Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
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BLAST of GH720473 vs. Lotus protein
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins)
Total hits: 1
Match NameE-valueIdentityDescription
chr4.CM2115.90.r2.m1.140e-8289.13+ phase: 0 [more]
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BLAST of GH720473 vs. Soybean peptides
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides)
Total hits: 3
Match NameE-valueIdentityDescription
Glyma20g02370.1|PACid:163152572.220e-8289.13[more]
Glyma20g02370.2|PACid:163152582.222e-8289.13[more]
Glyma20g02370.3|PACid:163152595.266e-6394.03[more]
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BLAST of GH720473 vs. SwissProt
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Swissprot)
Total hits: 2
Match NameE-valueIdentityDescription
NCKP1_ARATH7.358e-4179.61Protein NAP1 OS=Arabidopsis thaliana GN=NAP1 PE=1 ... [more]
NCKP1_ORYSJ4.464e-3872.82Probable protein NAP1 OS=Oryza sativa subsp. japon... [more]
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BLAST of GH720473 vs. Medicago proteins
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Medicago proteins)
Total hits: 1
Match NameE-valueIdentityDescription
IMGA|Medtr4g084140.12.183e-85100.00Nck-associated protein 1 (AHRD V1 **-- B7ZGK5_LOTJ... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v2
Date Performed: 2011-04-27
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019137Nck-associated protein 1PFAMPF09735Nckap1coord: 7..99
score: 6.6
IPR019137Nck-associated protein 1PFAMPF09735Nckap1coord: 8..97
score: 1.1
NoneNo IPR availablePANTHERPTHR12093FAMILY NOT NAMEDcoord: 1..103
score: 3.3
NoneNo IPR availablePANTHERPTHR12093:SF1SUBFAMILY NOT NAMEDcoord: 1..103
score: 3.3
NoneNo IPR availablePANTHERPTHR12093FAMILY NOT NAMEDcoord: 8..101
score: 9.8
NoneNo IPR availablePANTHERPTHR12093:SF1SUBFAMILY NOT NAMEDcoord: 8..101
score: 9.8
NoneNo IPR availableSEGsegsegcoord: 60..68
score

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019137Nck-associated protein 1PFAMPF09735Nckap1coord: 7..99
score: 6.6
IPR019137Nck-associated protein 1PFAMPF09735Nckap1coord: 8..97
score: 1.1
NoneNo IPR availablePANTHERPTHR12093FAMILY NOT NAMEDcoord: 1..103
score: 3.3
NoneNo IPR availablePANTHERPTHR12093:SF1SUBFAMILY NOT NAMEDcoord: 1..103
score: 3.3
NoneNo IPR availablePANTHERPTHR12093FAMILY NOT NAMEDcoord: 8..101
score: 9.8
NoneNo IPR availablePANTHERPTHR12093:SF1SUBFAMILY NOT NAMEDcoord: 8..101
score: 9.8
NoneNo IPR availableSEGsegsegcoord: 60..68
score

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v2
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>GH720473 ID=GH720473; Name=GH720473; organism=Pisum sativum; type=EST; length=584bp
TTCGAGCGGCCGCCCGNGGCAGGTACTTCCAACATGTTGGGGTTGCATCA
TCAAAATCTAAAACTGCTCGTGTGGTGCCAGTGGACATTGACCCAAATGA
CCCAACCATTGGGTTTCTGTTGGATGGAATGGACCATTTGTGTTGCTTGG
TCCGGAAATACATTGCACCGATTCGAGGTTACTCATTGTCTTATCTTTCA
TCCAGTGCCGGAATAATCCGATTTTTGCTGGGAACCCCTGTAATGGTGGC
TCTTGACATAGATCCTAGCTTGAAAGGACTTCTTCACCAGATTGTCCACC
ACCTCTGAACACTTGCCAAAACCTCAGAGTGAAAATATATCTGCTATCAC
TTGTGATCTGTCGGATTTTCGAAAGGACTGGCTATCAATTTTGCTGATGG
TCACGTCTTCGCGTTCATCTATAAACATTAGGCACTTGGAGAAAGCTACA
GTTTCCACCGGCAAAGAAGGCTTACTATCGGAGGGGAATGCTGCTTACAA
TTGGTCCAGATGCGTGGATGAATTGGAATCCCTACTTTCAAAGCATGGTA
GTCTTAGGAAGCTGTACCTCGGCCGCGACCACGC
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: INTERPRO
TermDefinition
IPR019137Nck-associated_protein-1