AA980044

EST Overview


Name	AA980044
Unique Name	AA980044
Type	EST
Organism	Pisum sativum (pea)
Sequence length	317

Libraries


Library Name	Type
LIBEST_001307 Pea mRNA/differential display	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of AA980044 vs. TrEMBL
Match: C0JJI2_SOYBN (Copia-type polyprotein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 3.655e-25
Identity = 49/67 (73.13%), Postives = 54/67 (80.60%), Query Frame = 2

Query:   77 HAQGXIERYKARLVAKGY*QXAGVDYDEVFAPXARMETIRLFISQAAQFKWPIFQMDVXTAFLNGVL 277
            +A+G +ER+KARLVAKGY Q   VDYDEVFAP ARMETIRL IS AAQ KW IFQ DV +AFLNG L
Sbjct:  580 NAKGEVERHKARLVAKGYKQQYEVDYDEVFAPVARMETIRLLISLAAQMKWRIFQFDVKSAFLNGYL 646

HSP 2 Score: 42.3578 bits (98), Expect = 3.655e-25
Identity = 18/24 (75.00%), Postives = 19/24 (79.17%), Query Frame = 1

Query:    1 NTWELVELPKGSQPIGVKWVFKKK 72
            NTWEL  LPKG + IGVKWVFK K
Sbjct:  555 NTWELSSLPKGHEAIGVKWVFKIK 578

[Close]

BLAST of AA980044 vs. TrEMBL
Match: Q9C739_ARATH (Copia-type polyprotein, putative OS=Arabidopsis thaliana GN=F11I4_21 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.360e-24
Identity = 48/67 (71.64%), Postives = 56/67 (83.58%), Query Frame = 2

Query:   77 HAQGXIERYKARLVAKGY*QXAGVDYDEVFAPXARMETIRLFISQAAQFKWPIFQMDVXTAFLNGVL 277
            +++G +ERYKARLVAKGY Q AG+DYDEVFAP AR+ET+RL IS AAQ KW I QMDV +AFLNG L
Sbjct:  890 NSKGEVERYKARLVAKGYIQRAGIDYDEVFAPVARLETVRLIISLAAQNKWKIHQMDVKSAFLNGDL 956

HSP 2 Score: 38.5058 bits (88), Expect = 1.360e-24
Identity = 15/24 (62.50%), Postives = 18/24 (75.00%), Query Frame = 1

Query:    1 NTWELVELPKGSQPIGVKWVFKKK 72
            +TWEL  LP G + IGVKWV+K K
Sbjct:  865 DTWELTSLPNGHKTIGVKWVYKAK 888

[Close]

BLAST of AA980044 vs. TrEMBL
Match: Q9C536_ARATH (Copia-type polyprotein, putative OS=Arabidopsis thaliana GN=T18I24.5 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.360e-24
Identity = 48/67 (71.64%), Postives = 56/67 (83.58%), Query Frame = 2

Query:   77 HAQGXIERYKARLVAKGY*QXAGVDYDEVFAPXARMETIRLFISQAAQFKWPIFQMDVXTAFLNGVL 277
            +++G +ERYKARLVAKGY Q AG+DYDEVFAP AR+ET+RL IS AAQ KW I QMDV +AFLNG L
Sbjct:  858 NSKGEVERYKARLVAKGYSQRAGIDYDEVFAPVARLETVRLIISLAAQNKWKIHQMDVKSAFLNGDL 924

HSP 2 Score: 38.5058 bits (88), Expect = 1.360e-24
Identity = 15/24 (62.50%), Postives = 18/24 (75.00%), Query Frame = 1

Query:    1 NTWELVELPKGSQPIGVKWVFKKK 72
            +TWEL  LP G + IGVKWV+K K
Sbjct:  833 DTWELTSLPNGHKAIGVKWVYKAK 856

[Close]

BLAST of AA980044 vs. TrEMBL
Match: Q9M197_ARATH (Copia-type reverse transcriptase-like protein OS=Arabidopsis thaliana GN=T16L24.270 PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 1.771e-24
Identity = 47/67 (70.15%), Postives = 56/67 (83.58%), Query Frame = 2

Query:   77 HAQGXIERYKARLVAKGY*QXAGVDYDEVFAPXARMETIRLFISQAAQFKWPIFQMDVXTAFLNGVL 277
            +++G +ERYKARLVAKGY Q AG+DYDE+FAP AR+ET+RL IS AAQ KW I QMDV +AFLNG L
Sbjct:  890 NSKGEVERYKARLVAKGYSQRAGIDYDEIFAPVARLETVRLIISLAAQNKWKIHQMDVKSAFLNGDL 956

HSP 2 Score: 38.5058 bits (88), Expect = 1.771e-24
Identity = 15/24 (62.50%), Postives = 18/24 (75.00%), Query Frame = 1

Query:    1 NTWELVELPKGSQPIGVKWVFKKK 72
            +TWEL  LP G + IGVKWV+K K
Sbjct:  865 DTWELTSLPNGHKAIGVKWVYKAK 888

[Close]

BLAST of AA980044 vs. TrEMBL
Match: Q9SXB2_ARATH (T28P6.8 protein OS=Arabidopsis thaliana GN=T28P6.8 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 3.900e-24
Identity = 47/67 (70.15%), Postives = 55/67 (82.09%), Query Frame = 2

Query:   77 HAQGXIERYKARLVAKGY*QXAGVDYDEVFAPXARMETIRLFISQAAQFKWPIFQMDVXTAFLNGVL 277
            +++G +ERYKARLVAKGY Q  G+DYDEVFAP AR+ET+RL IS AAQ KW I QMDV +AFLNG L
Sbjct:  890 NSKGEVERYKARLVAKGYSQRVGIDYDEVFAPVARLETVRLIISLAAQNKWKIHQMDVKSAFLNGDL 956

HSP 2 Score: 38.5058 bits (88), Expect = 3.900e-24
Identity = 15/24 (62.50%), Postives = 18/24 (75.00%), Query Frame = 1

Query:    1 NTWELVELPKGSQPIGVKWVFKKK 72
            +TWEL  LP G + IGVKWV+K K
Sbjct:  865 DTWELTSLPNGHKAIGVKWVYKAK 888

[Close]

BLAST of AA980044 vs. TrEMBL
Match: Q9M2D1_ARATH (Copia-type polyprotein OS=Arabidopsis thaliana GN=T20K12.230 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 3.900e-24
Identity = 47/67 (70.15%), Postives = 55/67 (82.09%), Query Frame = 2

Query:   77 HAQGXIERYKARLVAKGY*QXAGVDYDEVFAPXARMETIRLFISQAAQFKWPIFQMDVXTAFLNGVL 277
            +++G +ERYKARLVAKGY Q  G+DYDEVFAP AR+ET+RL IS AAQ KW I QMDV +AFLNG L
Sbjct:  890 NSKGEVERYKARLVAKGYSQRVGIDYDEVFAPVARLETVRLIISLAAQNKWKIHQMDVKSAFLNGDL 956

HSP 2 Score: 38.5058 bits (88), Expect = 3.900e-24
Identity = 15/24 (62.50%), Postives = 18/24 (75.00%), Query Frame = 1

Query:    1 NTWELVELPKGSQPIGVKWVFKKK 72
            +TWEL  LP G + IGVKWV+K K
Sbjct:  865 DTWELTSLPNGHKAIGVKWVYKAK 888

[Close]

BLAST of AA980044 vs. TrEMBL
Match: Q9SFE1_ARATH (T26F17.17 OS=Arabidopsis thaliana PE=4 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.119e-23
Identity = 46/65 (70.77%), Postives = 53/65 (81.54%), Query Frame = 2

Query:   77 HAQGXIERYKARLVAKGY*QXAGVDYDEVFAPXARMETIRLFISQAAQFKWPIFQMDVXTAFLNG 271
            +++G +ERYKARLVAKGY Q AG+DYDEVFAP AR+ET+RL IS AAQ KW I QMD   AFLNG
Sbjct:  829 NSKGEVERYKARLVAKGYSQRAGIDYDEVFAPVARLETVRLIISLAAQNKWKIHQMDFKLAFLNG 893

HSP 2 Score: 38.5058 bits (88), Expect = 1.119e-23
Identity = 15/24 (62.50%), Postives = 18/24 (75.00%), Query Frame = 1

Query:    1 NTWELVELPKGSQPIGVKWVFKKK 72
            +TWEL  LP G + IGVKWV+K K
Sbjct:  804 DTWELTSLPNGHKAIGVKWVYKAK 827

[Close]

BLAST of AA980044 vs. TrEMBL
Match: A5B902_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_032980 PE=4 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 2.027e-22
Identity = 44/64 (68.75%), Postives = 52/64 (81.25%), Query Frame = 2

Query:   86 GXIERYKARLVAKGY*QXAGVDYDEVFAPXARMETIRLFISQAAQFKWPIFQMDVXTAFLNGVL 277
            G +++YKARLVAKGY Q  GVDY EVFAP AR +TIRL I+ AAQ  WPIFQ+DVX+AFL+G L
Sbjct:  837 GEVDKYKARLVAKGYKQEFGVDYKEVFAPVARHDTIRLVIALAAQNSWPIFQLDVXSAFLHGNL 900

HSP 2 Score: 42.3578 bits (98), Expect = 2.027e-22
Identity = 17/25 (68.00%), Postives = 21/25 (84.00%), Query Frame = 1

Query:    1 NTWELVELPKGSQPIGVKWVFKKKM 75
            +TWEL ELPKG + IGVKWV+K K+
Sbjct:  809 DTWELSELPKGHKTIGVKWVYKTKL 833

[Close]

BLAST of AA980044 vs. TrEMBL
Match: A5B3I2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_004365 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 2.027e-22
Identity = 43/71 (60.56%), Postives = 55/71 (77.46%), Query Frame = 2

Query:   65 RRRCHAQGXIERYKARLVAKGY*QXAGVDYDEVFAPXARMETIRLFISQAAQFKWPIFQMDVXTAFLNGVL 277
            + + +  G +++YKARLVAKGY Q  GVDY EVFAP ARMETIRL ++ AAQ KW I+Q+DV +AFL+G L
Sbjct:  830 KTKFNENGEVDKYKARLVAKGYTQQHGVDYTEVFAPVARMETIRLVVALAAQRKWTIYQLDVKSAFLHGEL 900

HSP 2 Score: 40.4318 bits (93), Expect = 2.027e-22
Identity = 15/23 (65.22%), Postives = 19/23 (82.61%), Query Frame = 1

Query:    4 TWELVELPKGSQPIGVKWVFKKK 72
            TWEL ELP+G + IGVKW++K K
Sbjct:  810 TWELTELPEGGKKIGVKWIYKTK 832

[Close]

BLAST of AA980044 vs. TrEMBL
Match: Q19PL0_POPTR (NBS type disease resistance protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 2.035e-22
Identity = 45/67 (67.16%), Postives = 55/67 (82.09%), Query Frame = 2

Query:   77 HAQGXIERYKARLVAKGY*QXAGVDYDEVFAPXARMETIRLFISQAAQFKWPIFQMDVXTAFLNGVL 277
            +A+G ++RYKARLVAKGY Q  G+DY EVFAP AR+ETIRL IS AAQ +W I+Q+DV +AFLNG L
Sbjct:   37 NAKGEVQRYKARLVAKGYKQREGIDYGEVFAPVARLETIRLMISLAAQHRWKIYQLDVKSAFLNGFL 103

HSP 2 Score: 34.6538 bits (78), Expect = 2.035e-22
Identity = 13/24 (54.17%), Postives = 18/24 (75.00%), Query Frame = 1

Query:    1 NTWELVELPKGSQPIGVKWVFKKK 72
            +TW+L  LP+  + IGVKWV+K K
Sbjct:   12 DTWKLTYLPENKKAIGVKWVYKTK 35

[Close]

BLAST of AA980044 vs. SwissProt
Match: POLX_TOBAC (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 9.448e-16
Identity = 31/60 (51.67%), Postives = 42/60 (70.00%), Query Frame = 2

Query:   98 RYKARLVAKGY*QXAGVDYDEVFAPXARMETIRLFISQAAQFKWPIFQMDVXTAFLNGVL 277
            RYKARLV KG+ Q  G+D+DE+F+P  +M +IR  +S AA     + Q+DV TAFL+G L
Sbjct:  873 RYKARLVVKGFEQKKGIDFDEIFSPVVKMTSIRTILSLAASLDLEVEQLDVKTAFLHGDL 932

HSP 2 Score: 36.965 bits (84), Expect = 9.448e-16
Identity = 15/23 (65.22%), Postives = 19/23 (82.61%), Query Frame = 1

Query:    4 TWELVELPKGSQPIGVKWVFKKK 72
            T++LVELPKG +P+  KWVFK K
Sbjct:  842 TYKLVELPKGKRPLKCKWVFKLK 864

[Close]

BLAST of AA980044 vs. SwissProt
Match: COPIA_DROME (Copia protein OS=Drosophila melanogaster GN=GIP PE=1 SV=3)

HSP 1 Score: 67.781 bits (164), Expect = 6.514e-13
Identity = 32/60 (53.33%), Postives = 41/60 (68.33%), Query Frame = 2

Query:   98 RYKARLVAKGY*QXAGVDYDEVFAPXARMETIRLFISQAAQFKWPIFQMDVXTAFLNGVL 277
            RYKARLVA+G+ Q   +DY+E FAP AR+ + R  +S   Q+   + QMDV TAFLNG L
Sbjct:  953 RYKARLVARGFTQKYQIDYEETFAPVARISSFRFILSLVIQYNLKVHQMDVKTAFLNGTL 1012

HSP 2 Score: 25.0238 bits (53), Expect = 6.514e-13
Identity = 8/24 (33.33%), Postives = 13/24 (54.17%), Query Frame = 1

Query:    1 NTWELVELPKGSQPIGVKWVFKKK 72
            NTW + + P+    +  +WVF  K
Sbjct:  921 NTWTITKRPENKNIVDSRWVFSVK 944

[Close]

BLAST of AA980044 vs. SwissProt
Match: M820_ARATH (Uncharacterized mitochondrial protein AtMg00820 OS=Arabidopsis thaliana GN=AtMg00820 PE=4 SV=1)

HSP 1 Score: 46.9802 bits (110), Expect = 6.578e-8
Identity = 21/54 (38.89%), Postives = 34/54 (62.96%), Query Frame = 2

Query:   65 RRRCHAQGXIERYKARLVAKGY*QXAGVDYDEVFAPXARMETIRLFISQAAQFK 226
            + + H+ G ++R KARLVAKG+ Q  G+ + E ++P  R  TIR  ++ A Q +
Sbjct:   76 KTKLHSDGTLDRLKARLVAKGFHQEEGIYFVETYSPVVRTATIRTILNVAQQLE 129

HSP 2 Score: 28.8758 bits (63), Expect = 6.578e-8
Identity = 12/24 (50.00%), Postives = 14/24 (58.33%), Query Frame = 1

Query:    4 TWELVELPKGSQPIGVKWVFKKKM 75
            TW LV  P     +G KWVFK K+
Sbjct:   56 TWILVPPPVNQNILGCKWVFKTKL 79

[Close]

BLAST of AA980044 vs. TAIR peptide
Match: AT4G23160.1 (| Symbols: CRK8 | cysteine-rich RLK (RECEPTOR-like protein kinase) 8 | chr4:12129485-12134086 FORWARD LENGTH=1262)

HSP 1 Score: 68.1662 bits (165), Expect = 4.430e-17
Identity = 30/67 (44.78%), Postives = 48/67 (71.64%), Query Frame = 2

Query:   77 HAQGXIERYKARLVAKGY*QXAGVDYDEVFAPXARMETIRLFISQAAQFKWPIFQMDVXTAFLNGVL 277
            ++ G IERYKARLVAKGY Q  G+D+ E F+P  ++ +++L ++ +A + + + Q+D+  AFLNG L
Sbjct:  138 NSDGTIERYKARLVAKGYTQQEGIDFIETFSPVCKLTSVKLILAISAIYNFTLHQLDISNAFLNGDL 204

HSP 2 Score: 35.039 bits (79), Expect = 4.430e-17
Identity = 13/24 (54.17%), Postives = 17/24 (70.83%), Query Frame = 1

Query:    1 NTWELVELPKGSQPIGVKWVFKKK 72
            +TWE+  LP   +PIG KWV+K K
Sbjct:  113 HTWEICTLPPNKKPIGCKWVYKIK 136

[Close]

BLAST of AA980044 vs. TAIR peptide
Match: ATMG00820.1 (| Symbols: ORF170 | Reverse transcriptase (RNA-dependent DNA polymerase) | chrM:228573-229085 REVERSE LENGTH=170)

HSP 1 Score: 46.9802 bits (110), Expect = 5.205e-9
Identity = 21/54 (38.89%), Postives = 34/54 (62.96%), Query Frame = 2

Query:   65 RRRCHAQGXIERYKARLVAKGY*QXAGVDYDEVFAPXARMETIRLFISQAAQFK 226
            + + H+ G ++R KARLVAKG+ Q  G+ + E ++P  R  TIR  ++ A Q +
Sbjct:   76 KTKLHSDGTLDRLKARLVAKGFHQEEGIYFVETYSPVVRTATIRTILNVAQQLE 129

HSP 2 Score: 28.8758 bits (63), Expect = 5.205e-9
Identity = 12/24 (50.00%), Postives = 14/24 (58.33%), Query Frame = 1

Query:    4 TWELVELPKGSQPIGVKWVFKKKM 75
            TW LV  P     +G KWVFK K+
Sbjct:   56 TWILVPPPVNQNILGCKWVFKTKL 79

The following BLAST results are available for this feature:

BLAST of AA980044 vs. TrEMBL
BLAST of AA980044 vs. SwissProt
BLAST of AA980044 vs. TAIR peptide

BLAST of AA980044 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C0JJI2_SOYBN	3.655e-25	73.13	Copia-type polyprotein OS=Glycine max PE=4 SV=1	[more]
Q9C739_ARATH	1.360e-24	71.64	Copia-type polyprotein, putative OS=Arabidopsis th...	[more]
Q9C536_ARATH	1.360e-24	71.64	Copia-type polyprotein, putative OS=Arabidopsis th...	[more]
Q9M197_ARATH	1.771e-24	70.15	Copia-type reverse transcriptase-like protein OS=A...	[more]
Q9SXB2_ARATH	3.900e-24	70.15	T28P6.8 protein OS=Arabidopsis thaliana GN=T28P6.8...	[more]
Q9M2D1_ARATH	3.900e-24	70.15	Copia-type polyprotein OS=Arabidopsis thaliana GN=...	[more]
Q9SFE1_ARATH	1.119e-23	70.77	T26F17.17 OS=Arabidopsis thaliana PE=4 SV=1	[more]
A5B902_VITVI	2.027e-22	68.75	Putative uncharacterized protein OS=Vitis vinifera...	[more]
A5B3I2_VITVI	2.027e-22	60.56	Putative uncharacterized protein OS=Vitis vinifera...	[more]
Q19PL0_POPTR	2.035e-22	67.16	NBS type disease resistance protein OS=Populus tri...	[more]

BLAST of AA980044 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 3


Match Name	E-value	Identity	Description
POLX_TOBAC	9.448e-16	51.67	Retrovirus-related Pol polyprotein from transposon...	[more]
COPIA_DROME	6.514e-13	53.33	Copia protein OS=Drosophila melanogaster GN=GIP PE...	[more]
M820_ARATH	6.578e-8	38.89	Uncharacterized mitochondrial protein AtMg00820 OS...	[more]

BLAST of AA980044 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT4G23160.1	4.430e-17	44.78	\| Symbols: CRK8 \| cysteine-rich RLK (RECEPTOR-like...	[more]
ATMG00820.1	5.205e-9	38.89	\| Symbols: ORF170 \| Reverse transcriptase (RNA-dep...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013103	Reverse transcriptase, RNA-dependent DNA polymerase	PFAM	PF07727	RVT_2	coord: 1..35 score: 4.1
IPR013103	Reverse transcriptase, RNA-dependent DNA polymerase	PFAM	PF07727	RVT_2	coord: 1..49 score: 1.4
None	No IPR available	PANTHER	PTHR11439	GAG-POL-RELATED RETROTRANSPOSON	coord: 1..24 score: 0.0
None	No IPR available	PANTHER	PTHR11439	GAG-POL-RELATED RETROTRANSPOSON	coord: 1..48 score: 4

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AA980044 ID=AA980044; Name=AA980044; organism=Pisum sativum; type=EST; length=317bp
AACACTTGGGAGCTAGTNGAATTGCCAAAAGGTAGTCAACCAATTGGTGT
AAAGTGGGTATTCAAGAAGAAGATGCCACGCTCAAGGAGANATAGAACGA
TACAAAGCGAGACTTGTTGCGAAGGGATACTAACAGNAAGCAGGAGTTGA
TTATGACGAAGTATTTGCACCTNTTGCAAGAATGGAGACAATTCGATTAT
TCATATCTCAAGCTGCTCAATTCAAATGGCCAATATTTCAAATGGATGTC
NAAACAGCTTTTCTGAATGGTGTACTAGCCGCATGTAACCAAGGAATCGG
NGCCCCGGTACCGAAAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR013103	RVT_2

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