AJ308135

EST Overview


Name	AJ308135
Unique Name	AJ308135
Type	EST
Organism	Pisum sativum (pea)
Sequence length	305

Libraries


Library Name	Type
LIBEST_009888 SSH	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of AJ308135 vs. TrEMBL
Match: Q700A5_CICAR (Tonoplast intrinsic protein 2 (Fragment) OS=Cicer arietinum GN=Tip2 PE=2 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 3.241e-39
Identity = 79/89 (88.76%), Postives = 87/89 (97.75%), Query Frame = 1

Query:   37 SFVTGKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            ++VT KSVPTHGVAAGL+P+A LVFE+I+TFGLVYTVYATAADPKKGSLGTIAPIAIGF+VGANILVAGPFSGGSMNPARSFGPAVV+G
Sbjct:   45 NYVTSKSVPTHGVAAGLSPIAGLVFEIIVTFGLVYTVYATAADPKKGSLGTIAPIAIGFIVGANILVAGPFSGGSMNPARSFGPAVVSG 133

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BLAST of AJ308135 vs. TrEMBL
Match: A0RZG9_FRAAN (Putative tonoplast intrinsic protein OS=Fragaria ananassa GN=FavRB7 PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 7.471e-36
Identity = 73/89 (82.02%), Postives = 83/89 (93.26%), Query Frame = 1

Query:   37 SFVTGKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            +FVT +SVPTHG+A+G+  +  +VFE+IITFGLVYTVYATAADPKKGSLGTIAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAVV+G
Sbjct:  120 NFVTSESVPTHGLASGVGAIEGIVFEIIITFGLVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVVSG 208

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BLAST of AJ308135 vs. TrEMBL
Match: D7MPT7_ARALY (AtTIP2_3 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_494233 PE=4 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 8.260e-35
Identity = 74/89 (83.15%), Postives = 81/89 (91.01%), Query Frame = 1

Query:   40 FVT-GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            FVT GKSVPTHGVAAGL  +  +V E+++TF LVYTVYATAADPKKGSLGTIAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAVV+G
Sbjct:  121 FVTNGKSVPTHGVAAGLGAVEGIVMEIVVTFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVVSG 209

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BLAST of AJ308135 vs. TrEMBL
Match: D7KQV6_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_475524 PE=4 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 8.260e-35
Identity = 74/89 (83.15%), Postives = 81/89 (91.01%), Query Frame = 1

Query:   40 FVT-GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            FVT GKSVPTHGVAAGL  +  +V E+++TF LVYTVYATAADPKKGSLGTIAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAVV+G
Sbjct:  226 FVTNGKSVPTHGVAAGLGAVEGIVMEIVVTFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVVSG 314

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BLAST of AJ308135 vs. TrEMBL
Match: Q2A9D7_BRAOL (Tonoplast intrinsic protein, putative OS=Brassica oleracea GN=31.t00034 PE=3 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 2.403e-34
Identity = 73/89 (82.02%), Postives = 81/89 (91.01%), Query Frame = 1

Query:   40 FVT-GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            FVT G+SVPTHGVAAGL  +  +V E+++TF LVYTVYATAADPKKGSLGTIAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAVV+G
Sbjct:  121 FVTIGESVPTHGVAAGLGAVEGIVMEIVVTFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVVSG 209

[Close]

BLAST of AJ308135 vs. TrEMBL
Match: D7MC03_ARALY (DELTA-TIP2/TIP2_2 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_493143 PE=4 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 2.403e-34
Identity = 73/89 (82.02%), Postives = 81/89 (91.01%), Query Frame = 1

Query:   40 FVT-GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            FVT G+SVPTHGVAAGL  +  +V E+++TF LVYTVYATAADPKKGSLGTIAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAVV+G
Sbjct:  121 FVTNGESVPTHGVAAGLGAIEGVVMEIVVTFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVVSG 209

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BLAST of AJ308135 vs. TrEMBL
Match: Q107R6_PHAVU (Aquaporin OS=Phaseolus vulgaris GN=Aqp1 PE=2 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 3.139e-34
Identity = 72/89 (80.90%), Postives = 79/89 (88.76%), Query Frame = 1

Query:   37 SFVTGKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            + VT KS+PTHG A G+N    +V E+IITFGLVYTVYATAADPKKGSLGTIAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAVV+G
Sbjct:  120 NLVTAKSIPTHGPAYGVNAFQGVVCEIIITFGLVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVVSG 208

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BLAST of AJ308135 vs. TrEMBL
Match: C8YXI4_DAUCA (Tonoplast aquaporin 2;1 OS=Daucus carota GN=TIP2;1 PE=2 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 3.139e-34
Identity = 74/89 (83.15%), Postives = 80/89 (89.89%), Query Frame = 1

Query:   40 FVTG-KSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            FVTG K+VPTHGV AGL+    +VFE+IITF LVYTVYATAADPKKGSLGTIAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAV +G
Sbjct:  121 FVTGGKAVPTHGVGAGLDAAEGVVFEIIITFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVASG 209

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BLAST of AJ308135 vs. TrEMBL
Match: C5IW58_LOLPR (Tonoplast intrinsic protein 2;1 OS=Lolium perenne GN=TIP2;1 PE=2 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 3.139e-34
Identity = 71/88 (80.68%), Postives = 79/88 (89.77%), Query Frame = 1

Query:   40 FVTGKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            F TG + PTHG++AG+  L  +V EVI+TFGLVYTVYATAADPKKGSLGTIAPIAIGF+VGANILVAGPFSGGSMNPARSFGPAV +G
Sbjct:  122 FCTGMATPTHGLSAGVGALEGVVMEVIVTFGLVYTVYATAADPKKGSLGTIAPIAIGFIVGANILVAGPFSGGSMNPARSFGPAVASG 209

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BLAST of AJ308135 vs. TrEMBL
Match: C6T404_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 1.193e-33
Identity = 69/89 (77.53%), Postives = 78/89 (87.64%), Query Frame = 1

Query:   37 SFVTGKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            +F+T KS+P+H  A G+N    +VFE++ITFGLVYTVYATAADPKKGSLG IAPIAIGFVVGANIL AGPFSGGSMNPARSFGPA V+G
Sbjct:  120 NFITAKSIPSHAPATGVNDFQAVVFEIVITFGLVYTVYATAADPKKGSLGIIAPIAIGFVVGANILAAGPFSGGSMNPARSFGPAAVSG 208

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BLAST of AJ308135 vs. SwissProt
Match: TIP23_ARATH (Aquaporin TIP2-3 OS=Arabidopsis thaliana GN=TIP2-3 PE=1 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 1.162e-35
Identity = 73/89 (82.02%), Postives = 81/89 (91.01%), Query Frame = 1

Query:   40 FVT-GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            FVT GKSVPTHGV+AGL  +  +V E+++TF LVYTVYATAADPKKGSLGTIAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAVV+G
Sbjct:  121 FVTNGKSVPTHGVSAGLGAVEGVVMEIVVTFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVVSG 209

[Close]

BLAST of AJ308135 vs. SwissProt
Match: TIP22_ARATH (Probable aquaporin TIP2-2 OS=Arabidopsis thaliana GN=TIP2-2 PE=1 SV=2)

HSP 1 Score: 148.288 bits (373), Expect = 1.162e-35
Identity = 73/89 (82.02%), Postives = 81/89 (91.01%), Query Frame = 1

Query:   40 FVT-GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            FVT G+SVPTHGVAAGL  +  +V E+++TF LVYTVYATAADPKKGSLGTIAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAVV+G
Sbjct:  121 FVTNGESVPTHGVAAGLGAIEGVVMEIVVTFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVVSG 209

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BLAST of AJ308135 vs. SwissProt
Match: TIP1_TOBAC (Probable aquaporin TIP-type RB7-5A OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 2.588e-35
Identity = 73/85 (85.88%), Postives = 77/85 (90.59%), Query Frame = 1

Query:   49 GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            G +VPTHGVAAGLN L  +V E+IITF LVYTVYATAADPKKGSLGTIAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAVV G
Sbjct:  125 GLAVPTHGVAAGLNGLQGVVMEIIITFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVVAG 209

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BLAST of AJ308135 vs. SwissProt
Match: TIP2_TOBAC (Probable aquaporin TIP-type RB7-18C OS=Nicotiana tabacum PE=2 SV=2)

HSP 1 Score: 145.591 bits (366), Expect = 7.531e-35
Identity = 72/85 (84.71%), Postives = 76/85 (89.41%), Query Frame = 1

Query:   49 GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            G +VPTHGVAAGLN    +V E+IITF LVYTVYATAADPKKGSLGTIAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAVV G
Sbjct:  125 GLAVPTHGVAAGLNGFQGVVMEIIITFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVVAG 209

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BLAST of AJ308135 vs. SwissProt
Match: TIP_ANTMA (Probable aquaporin TIP-type OS=Antirrhinum majus GN=DIP PE=2 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 2.191e-34
Identity = 72/89 (80.90%), Postives = 79/89 (88.76%), Query Frame = 1

Query:   40 FVT-GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            FVT G SVPTHGVAAG++ +  +V E+IITF LVYTVYATAADPKKGSLG IAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAV +G
Sbjct:  121 FVTNGLSVPTHGVAAGMDAIQGVVMEIIITFALVYTVYATAADPKKGSLGVIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVASG 209

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BLAST of AJ308135 vs. SwissProt
Match: TIP22_MAIZE (Aquaporin TIP2-2 OS=Zea mays GN=TIP2-2 PE=2 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 4.881e-34
Identity = 70/86 (81.40%), Postives = 77/86 (89.53%), Query Frame = 1

Query:   40 FVT-GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAV 294
            FVT GK++PTHGV+ G   L  +VFE++ITF LVYTVYATAADPKKGSLGTIAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAV
Sbjct:  121 FVTHGKAIPTHGVSGGTTELEGVVFEIVITFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAV 206

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BLAST of AJ308135 vs. SwissProt
Match: TIP21_MAIZE (Aquaporin TIP2-1 OS=Zea mays GN=TIP2-1 PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 2.423e-33
Identity = 72/89 (80.90%), Postives = 79/89 (88.76%), Query Frame = 1

Query:   40 FVT-GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            FVT GK++PTH VA G++ L  +VFEV+ITF LVYTVYATAADPKKGSLGTIAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAV  G
Sbjct:  121 FVTHGKAIPTHAVA-GISELEGVVFEVVITFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVAAG 208

[Close]

BLAST of AJ308135 vs. SwissProt
Match: TIP21_ORYSJ (Probable aquaporin TIP2-1 OS=Oryza sativa subsp. japonica GN=TIP2-1 PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 3.164e-33
Identity = 71/89 (79.78%), Postives = 79/89 (88.76%), Query Frame = 1

Query:   40 FVT-GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            FVT GK++PTHGVA G++ L  +V E++ITF LVYTVYATAADPKKGSLGTIAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAV  G
Sbjct:  121 FVTHGKAIPTHGVA-GISELEGVVMEIVITFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVAAG 208

[Close]

BLAST of AJ308135 vs. SwissProt
Match: TIP22_ORYSJ (Probable aquaporin TIP2-2 OS=Oryza sativa subsp. japonica GN=TIP2-2 PE=2 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 5.397e-33
Identity = 68/88 (77.27%), Postives = 77/88 (87.50%), Query Frame = 1

Query:   40 FVTGKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            F TG + PTHG++ G+     +V E+I+TFGLVYTVYATAADPKKGSLGTIAPIAIGF+VGANILVAGPFSGGSMNPARSFGPAV +G
Sbjct:  122 FCTGVATPTHGLS-GVGAFEGVVMEIIVTFGLVYTVYATAADPKKGSLGTIAPIAIGFIVGANILVAGPFSGGSMNPARSFGPAVASG 208

[Close]

BLAST of AJ308135 vs. SwissProt
Match: TIP21_ARATH (Aquaporin TIP2-1 OS=Arabidopsis thaliana GN=TIP2-1 PE=1 SV=2)

HSP 1 Score: 137.502 bits (345), Expect = 2.051e-32
Identity = 67/85 (78.82%), Postives = 73/85 (85.88%), Query Frame = 1

Query:   49 GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            G +VPTH VAAGL  +  +V E+IITF LVYTVYATAADPKKGSLGTIAP+AIG +VGANIL AGPFSGGSMNPARSFGPAV  G
Sbjct:  125 GLAVPTHSVAAGLGSIEGVVMEIIITFALVYTVYATAADPKKGSLGTIAPLAIGLIVGANILAAGPFSGGSMNPARSFGPAVAAG 209

[Close]

BLAST of AJ308135 vs. TAIR peptide
Match: AT5G47450.1 (| Symbols: ATTIP2;3, TIP2;3, DELTA-TIP3 | tonoplast intrinsic protein 2;3 | chr5:19248509-19249466 REVERSE LENGTH=250)

HSP 1 Score: 148.288 bits (373), Expect = 9.161e-37
Identity = 73/89 (82.02%), Postives = 81/89 (91.01%), Query Frame = 1

Query:   40 FVT-GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            FVT GKSVPTHGV+AGL  +  +V E+++TF LVYTVYATAADPKKGSLGTIAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAVV+G
Sbjct:  121 FVTNGKSVPTHGVSAGLGAVEGVVMEIVVTFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVVSG 209

[Close]

BLAST of AJ308135 vs. TAIR peptide
Match: AT4G17340.1 (| Symbols: TIP2;2, DELTA-TIP2 | tonoplast intrinsic protein 2;2 | chr4:9699318-9700250 FORWARD LENGTH=250)

HSP 1 Score: 148.288 bits (373), Expect = 9.161e-37
Identity = 73/89 (82.02%), Postives = 81/89 (91.01%), Query Frame = 1

Query:   40 FVT-GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            FVT G+SVPTHGVAAGL  +  +V E+++TF LVYTVYATAADPKKGSLGTIAPIAIGF+VGANIL AGPFSGGSMNPARSFGPAVV+G
Sbjct:  121 FVTNGESVPTHGVAAGLGAIEGVVMEIVVTFALVYTVYATAADPKKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVVSG 209

[Close]

BLAST of AJ308135 vs. TAIR peptide
Match: AT3G16240.1 (| Symbols: DELTA-TIP, TIP2;1, DELTA-TIP1, AQP1, ATTIP2;1 | delta tonoplast integral protein | chr3:5505534-5506788 FORWARD LENGTH=250)

HSP 1 Score: 137.502 bits (345), Expect = 1.617e-33
Identity = 67/85 (78.82%), Postives = 73/85 (85.88%), Query Frame = 1

Query:   49 GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            G +VPTH VAAGL  +  +V E+IITF LVYTVYATAADPKKGSLGTIAP+AIG +VGANIL AGPFSGGSMNPARSFGPAV  G
Sbjct:  125 GLAVPTHSVAAGLGSIEGVVMEIIITFALVYTVYATAADPKKGSLGTIAPLAIGLIVGANILAAGPFSGGSMNPARSFGPAVAAG 209

[Close]

BLAST of AJ308135 vs. TAIR peptide
Match: AT3G26520.1 (| Symbols: TIP2, SITIP, GAMMA-TIP2, TIP1;2 | tonoplast intrinsic protein 2 | chr3:9722770-9723703 REVERSE LENGTH=253)

HSP 1 Score: 125.561 bits (314), Expect = 6.359e-30
Identity = 62/89 (69.66%), Postives = 73/89 (82.02%), Query Frame = 1

Query:   37 SFVTG-KSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVN 300
            SF TG + +P  G++AG+  L  LVFE+++TFGLVYTVYATA DPK GSLGTIAPIAIGF+VGANIL  G FSG SMNPA +FGPAVV+
Sbjct:  123 SFATGGEPIPAFGLSAGVGSLNALVFEIVMTFGLVYTVYATAVDPKNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVS 211

[Close]

BLAST of AJ308135 vs. TAIR peptide
Match: AT2G36830.1 (| Symbols: GAMMA-TIP, TIP1;1, GAMMA-TIP1 | gamma tonoplast intrinsic protein | chr2:15445490-15446336 FORWARD LENGTH=251)

HSP 1 Score: 122.094 bits (305), Expect = 7.031e-29
Identity = 59/84 (70.24%), Postives = 69/84 (82.14%), Query Frame = 1

Query:   49 GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVN 300
            G +VP  G++AG+  L   VFE+++TFGLVYTVYATA DPK GSLGTIAPIAIGF+VGANIL  G FSG SMNPA +FGPAVV+
Sbjct:  127 GLAVPAFGLSAGVGVLNAFVFEIVMTFGLVYTVYATAIDPKNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVS 210

[Close]

BLAST of AJ308135 vs. TAIR peptide
Match: AT4G01470.1 (| Symbols: GAMMA-TIP3, TIP1;3, ATTIP1.3 | tonoplast intrinsic protein 1;3 | chr4:625092-625850 REVERSE LENGTH=252)

HSP 1 Score: 110.153 bits (274), Expect = 2.765e-25
Identity = 52/84 (61.90%), Postives = 63/84 (75.00%), Query Frame = 1

Query:   49 GKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVN 300
            G       ++ G+ P   +VFE+++TFGLVYTVYATA DPKKG +G IAP+AIG +VGANILV G F G SMNPA SFGPAVV+
Sbjct:  127 GMETAAFSLSYGVTPWNAVVFEIVMTFGLVYTVYATAVDPKKGDIGIIAPLAIGLIVGANILVGGAFDGASMNPAVSFGPAVVS 210

[Close]

BLAST of AJ308135 vs. TAIR peptide
Match: AT1G73190.1 (| Symbols: ALPHA-TIP, TIP3;1 | Aquaporin-like superfamily protein | chr1:27522155-27523171 FORWARD LENGTH=268)

HSP 1 Score: 109.768 bits (273), Expect = 3.611e-25
Identity = 51/75 (68.00%), Postives = 63/75 (84.00%), Query Frame = 1

Query:   73 VAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVV 297
            +A+G+  +  LV E+I+TFGLVY VY+T  DPK+GSLG IAP+AIG +VGANILV GPFSG SMNPAR+FGPA+V
Sbjct:  143 LASGVGAVNGLVLEIILTFGLVYVVYSTLIDPKRGSLGIIAPLAIGLIVGANILVGGPFSGASMNPARAFGPALV 217

[Close]

BLAST of AJ308135 vs. TAIR peptide
Match: AT1G17810.1 (| Symbols: BETA-TIP | beta-tonoplast intrinsic protein | chr1:6130209-6131442 FORWARD LENGTH=267)

HSP 1 Score: 108.612 bits (270), Expect = 8.044e-25
Identity = 50/75 (66.67%), Postives = 63/75 (84.00%), Query Frame = 1

Query:   73 VAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVV 297
            VA+G++ L  L+ E+I+TF LVY VY+TA DPK+GS+G IAP+AIG +VGANILV GPF G SMNPAR+FGPA+V
Sbjct:  143 VASGVSELHGLLMEIILTFALVYVVYSTAIDPKRGSIGIIAPLAIGLIVGANILVGGPFDGASMNPARAFGPALV 217

[Close]

BLAST of AJ308135 vs. TAIR peptide
Match: AT2G25810.1 (| Symbols: TIP4;1 | tonoplast intrinsic protein 4;1 | chr2:11012658-11013906 FORWARD LENGTH=249)

HSP 1 Score: 107.071 bits (266), Expect = 2.341e-24
Identity = 51/90 (56.67%), Postives = 67/90 (74.44%), Query Frame = 1

Query:   37 SFVTG-KSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            S++TG    P H +A+G++    +++E+I+TF L++TVYAT  DPKKGSL    P+  GFVVGANIL  G FSG SMNPARSFGPA+V+G
Sbjct:  116 SYLTGGMGTPVHTLASGVSYTQGIIWEIILTFSLLFTVYATIVDPKKGSLDGFGPLLTGFVVGANILAGGAFSGASMNPARSFGPALVSG 205

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BLAST of AJ308135 vs. TAIR peptide
Match: AT1G52180.1 (| Symbols: | Aquaporin-like superfamily protein | chr1:19424944-19425928 REVERSE LENGTH=124)

HSP 1 Score: 105.916 bits (263), Expect = 5.214e-24
Identity = 51/86 (59.30%), Postives = 61/86 (70.93%), Query Frame = 1

Query:   46 TGKSVPTHGVAAGLNPLAXLVFEVIITFGLVYTVYATAADPKKGSLGTIAPIAIGFVVGANILVAGPFSGGSMNPARSFGPAVVNG 303
            T  ++P H VA  +     +V E+IITF LVYTVYATA D   G+LGTIAP+AI  +VGANIL AGPFSGG MNP RSFG ++  G
Sbjct:   34 TSNAIPIHSVAVRVGSTQRVVMEIIITFALVYTVYATAIDSNNGTLGTIAPLAIRLIVGANILAAGPFSGGPMNPGRSFGSSLAVG 119

The following BLAST results are available for this feature:

BLAST of AJ308135 vs. TrEMBL
BLAST of AJ308135 vs. SwissProt
BLAST of AJ308135 vs. TAIR peptide

BLAST of AJ308135 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q700A5_CICAR	3.241e-39	88.76	Tonoplast intrinsic protein 2 (Fragment) OS=Cicer ...	[more]
A0RZG9_FRAAN	7.471e-36	82.02	Putative tonoplast intrinsic protein OS=Fragaria a...	[more]
D7MPT7_ARALY	8.260e-35	83.15	AtTIP2_3 OS=Arabidopsis lyrata subsp. lyrata GN=AR...	[more]
D7KQV6_ARALY	8.260e-35	83.15	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
Q2A9D7_BRAOL	2.403e-34	82.02	Tonoplast intrinsic protein, putative OS=Brassica ...	[more]
D7MC03_ARALY	2.403e-34	82.02	DELTA-TIP2/TIP2_2 OS=Arabidopsis lyrata subsp. lyr...	[more]
Q107R6_PHAVU	3.139e-34	80.90	Aquaporin OS=Phaseolus vulgaris GN=Aqp1 PE=2 SV=1	[more]
C8YXI4_DAUCA	3.139e-34	83.15	Tonoplast aquaporin 2;1 OS=Daucus carota GN=TIP2;1...	[more]
C5IW58_LOLPR	3.139e-34	80.68	Tonoplast intrinsic protein 2;1 OS=Lolium perenne ...	[more]
C6T404_SOYBN	1.193e-33	77.53	Putative uncharacterized protein OS=Glycine max PE...	[more]

BLAST of AJ308135 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
TIP23_ARATH	1.162e-35	82.02	Aquaporin TIP2-3 OS=Arabidopsis thaliana GN=TIP2-3...	[more]
TIP22_ARATH	1.162e-35	82.02	Probable aquaporin TIP2-2 OS=Arabidopsis thaliana ...	[more]
TIP1_TOBAC	2.588e-35	85.88	Probable aquaporin TIP-type RB7-5A OS=Nicotiana ta...	[more]
TIP2_TOBAC	7.531e-35	84.71	Probable aquaporin TIP-type RB7-18C OS=Nicotiana t...	[more]
TIP_ANTMA	2.191e-34	80.90	Probable aquaporin TIP-type OS=Antirrhinum majus G...	[more]
TIP22_MAIZE	4.881e-34	81.40	Aquaporin TIP2-2 OS=Zea mays GN=TIP2-2 PE=2 SV=1	[more]
TIP21_MAIZE	2.423e-33	80.90	Aquaporin TIP2-1 OS=Zea mays GN=TIP2-1 PE=2 SV=1	[more]
TIP21_ORYSJ	3.164e-33	79.78	Probable aquaporin TIP2-1 OS=Oryza sativa subsp. j...	[more]
TIP22_ORYSJ	5.397e-33	77.27	Probable aquaporin TIP2-2 OS=Oryza sativa subsp. j...	[more]
TIP21_ARATH	2.051e-32	78.82	Aquaporin TIP2-1 OS=Arabidopsis thaliana GN=TIP2-1...	[more]

BLAST of AJ308135 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT5G47450.1	9.161e-37	82.02	\| Symbols: ATTIP2;3, TIP2;3, DELTA-TIP3 \| tonoplas...	[more]
AT4G17340.1	9.161e-37	82.02	\| Symbols: TIP2;2, DELTA-TIP2 \| tonoplast intrinsi...	[more]
AT3G16240.1	1.617e-33	78.82	\| Symbols: DELTA-TIP, TIP2;1, DELTA-TIP1, AQP1, AT...	[more]
AT3G26520.1	6.359e-30	69.66	\| Symbols: TIP2, SITIP, GAMMA-TIP2, TIP1;2 \| tonop...	[more]
AT2G36830.1	7.031e-29	70.24	\| Symbols: GAMMA-TIP, TIP1;1, GAMMA-TIP1 \| gamma t...	[more]
AT4G01470.1	2.765e-25	61.90	\| Symbols: GAMMA-TIP3, TIP1;3, ATTIP1.3 \| tonoplas...	[more]
AT1G73190.1	3.611e-25	68.00	\| Symbols: ALPHA-TIP, TIP3;1 \| Aquaporin-like supe...	[more]
AT1G17810.1	8.044e-25	66.67	\| Symbols: BETA-TIP \| beta-tonoplast intrinsic pro...	[more]
AT2G25810.1	2.341e-24	56.67	\| Symbols: TIP4;1 \| tonoplast intrinsic protein 4;...	[more]
AT1G52180.1	5.214e-24	59.30	\| Symbols: \| Aquaporin-like superfamily protein \|...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PRINTS	PR00783	MINTRINSICP	coord: 37..55 score: 2.9e-13coord: 70..92 score: 2.9
IPR000425	Major intrinsic protein	GENE3D	1.20.1080.10	no description	coord: 1..100 score: 6
IPR000425	Major intrinsic protein	PANTHER	PTHR19139	AQUAPORIN TRANSPORTER	coord: 1..101 score: 4.5
IPR000425	Major intrinsic protein	PFAM	PF00230	MIP	coord: 1..100 score: 1.2
IPR000425	Major intrinsic protein	SUPERFAMILY	81338	Aquaporin-like	coord: 1..100 score: 1.9
None	No IPR available	PANTHER	PTHR19139:SF30	TONOPLAST INTRINSIC PROTEIN	coord: 1..101 score: 4.5
None	No IPR available	SEG	seg	seg	coord: 2..12 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AJ308135 ID=AJ308135; Name=AJ308135; organism=Pisum sativum; type=EST; length=305bp
CAATTACTCGGCGCAATCCTTGVATCTCTCCTCCTCAGCTTCGTCACCGG
AAAGAGTGTTCCAACACATGGAGTTGCTGCTGGATTGAACCCTCTTGCAA
RATTAGTGTTTGAGGTTATTATTACATTTGGGTTGGTTTACACTGTTTAT
GCCACAGCAGCTGACCCCAAAAAGGGTTCATTAGGAACCATTGCACCAAT
CGCCATTGGGTTTGTTGTGGGTGCCAACATCTTAGTGGCCGGTCCATTCA
GCGGCGGGTCAATGAACCCGGCTCGCTCATTCGGTCCAGCTGTGGTTAAT
GGAAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005215	transporter activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006810	transport

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR000425	MIP

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