GH719623

EST Overview


Name	GH719623
Unique Name	GH719623
Type	EST
Organism	Pisum sativum (pea)
Sequence length	472

Libraries


Library Name	Type
LIBEST_024268 TI	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of GH719623 vs. TrEMBL
Match: D7MAZ7_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_328051 PE=4 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 4.601e-59
Identity = 89/126 (70.63%), Postives = 108/126 (85.71%), Query Frame = -3

Query:    3 KVKGLPFNRYSWLTSHNSFALAGARSATGSIIIAPMNQDDTIADQLKNGVRGFMLDMYDFQNAIWLCHSTQNQCFNFTSFQPAINALKDMRSFLDTNPSEIITIFIEDYVKSPSGLTKLFQASGIN 380
            KV GLPFN+YSWLT+HNS+A+ GA SATGS +I+P NQ+D+I +QLKNGVRG MLD YDFQN IWLCHST   CFNFT+FQPAINALK++  FL++N SEI+TI +EDYVKSP GLTK+F ASG++
Sbjct:  384 KVNGLPFNKYSWLTTHNSYAITGANSATGSFLISPKNQEDSITNQLKNGVRGIMLDTYDFQNDIWLCHSTGGTCFNFTAFQPAINALKEINDFLESNLSEIVTIILEDYVKSPMGLTKVFNASGLS 509

HSP 2 Score: 55.0694 bits (131), Expect = 4.601e-59
Identity = 22/31 (70.97%), Postives = 26/31 (83.87%), Query Frame = -2

Query:  379 CDVGLTCQTCPANGNTRPRCSRIQTLNPTTK 471
            CD GL+CQ+CPANGNT   C+RIQ LNPT+K
Sbjct:  354 CDAGLSCQSCPANGNTGSTCTRIQPLNPTSK 384

[Close]

BLAST of GH719623 vs. TrEMBL
Match: B9N4Z2_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_744317 PE=4 SV=1)

HSP 1 Score: 197.978 bits (502), Expect = 5.043e-58
Identity = 90/125 (72.00%), Postives = 106/125 (84.80%), Query Frame = -3

Query:    6 KVKGLPFNRYSWLTSHNSFALAGARSATGSIIIAPMNQDDTIADQLKNGVRGFMLDMYDFQNAIWLCHSTQNQCFNFTSFQPAINALKDMRSFLDTNPSEIITIFIEDYVKSPSGLTKLFQASGI 380
            K KGLPFN+Y+WLT+HNSFA  G RSATGSII+AP NQ DT+  QL NG+RGFMLDMYDFQN IWLCHS    C+NFT+FQPAIN LK++++FL+ NPSEIITIFIEDYV SP GLTK+F A+G+
Sbjct:   67 KEKGLPFNQYTWLTTHNSFAKLGDRSATGSIILAPTNQQDTVTSQLNNGIRGFMLDMYDFQNDIWLCHSFGGNCYNFTAFQPAINVLKEIQAFLEANPSEIITIFIEDYVTSPRGLTKVFDAAGL 191

HSP 2 Score: 50.8322 bits (120), Expect = 5.043e-58
Identity = 21/31 (67.74%), Postives = 25/31 (80.65%), Query Frame = -2

Query:  379 CDVGLTCQTCPANGNTRPRCSRIQTLNPTTK 471
            C+ GL C+TC ANGN RPRC+RIQ L PT+K
Sbjct:   37 CNSGLHCETCVANGNVRPRCTRIQPLIPTSK 67

[Close]

BLAST of GH719623 vs. TrEMBL
Match: O23190_ARATH (MAP3K-like protein kinase OS=Arabidopsis thaliana GN=C7A10.410 PE=4 SV=2)

HSP 1 Score: 191.815 bits (486), Expect = 1.887e-57
Identity = 86/126 (68.25%), Postives = 106/126 (84.13%), Query Frame = -3

Query:    3 KVKGLPFNRYSWLTSHNSFALAGARSATGSIIIAPMNQDDTIADQLKNGVRGFMLDMYDFQNAIWLCHSTQNQCFNFTSFQPAINALKDMRSFLDTNPSEIITIFIEDYVKSPSGLTKLFQASGIN 380
            KV GLPFN+YSWLT+HNS+A+ GA SATGS +++P NQ+D+I +QLKNGVRG MLD YDFQN IWLCHST   CFNFT+FQPAINALK++  FL++N SEI+TI +EDYVKS  GLT +F ASG++
Sbjct:  379 KVNGLPFNKYSWLTTHNSYAITGANSATGSFLVSPKNQEDSITNQLKNGVRGIMLDTYDFQNDIWLCHSTGGTCFNFTAFQPAINALKEINDFLESNLSEIVTIILEDYVKSQMGLTNVFNASGLS 504

HSP 2 Score: 55.0694 bits (131), Expect = 1.887e-57
Identity = 22/31 (70.97%), Postives = 26/31 (83.87%), Query Frame = -2

Query:  379 CDVGLTCQTCPANGNTRPRCSRIQTLNPTTK 471
            CD GL+CQ+CPANGNT   C+RIQ LNPT+K
Sbjct:  349 CDAGLSCQSCPANGNTGSTCTRIQPLNPTSK 379

[Close]

BLAST of GH719623 vs. TrEMBL
Match: B9H6H1_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_208603 PE=4 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 2.069e-56
Identity = 87/125 (69.60%), Postives = 102/125 (81.60%), Query Frame = -3

Query:    6 KVKGLPFNRYSWLTSHNSFALAGARSATGSIIIAPMNQDDTIADQLKNGVRGFMLDMYDFQNAIWLCHSTQNQCFNFTSFQPAINALKDMRSFLDTNPSEIITIFIEDYVKSPSGLTKLFQASGI 380
            KVKGL FN+YSWLT+HNSFAL  A+S TGS +IA  NQ+DT+  QLKNGVRG MLDMYDF N IWLCHS    C+NFT+FQPAI+ LK++ +FL  NPSEI+TIFIEDYV SP GLTK+F ASG+
Sbjct:   40 KVKGLAFNKYSWLTTHNSFALTNAQSDTGSALIATKNQEDTVTSQLKNGVRGLMLDMYDFMNDIWLCHSFNGNCYNFTAFQPAIDVLKEIETFLAANPSEIVTIFIEDYVTSPQGLTKVFNASGL 164

HSP 2 Score: 53.9138 bits (128), Expect = 2.069e-56
Identity = 21/31 (67.74%), Postives = 27/31 (87.10%), Query Frame = -2

Query:  379 CDVGLTCQTCPANGNTRPRCSRIQTLNPTTK 471
            CD GL+CQ+C ANGNTR RC++IQ L+PT+K
Sbjct:   10 CDAGLSCQSCSANGNTRQRCTKIQPLSPTSK 40

[Close]

BLAST of GH719623 vs. TrEMBL
Match: C6TH52_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 3.857e-55
Identity = 82/113 (72.57%), Postives = 96/113 (84.96%), Query Frame = -3

Query:   42 KVKGLPFNRYSWLTSHNSFALAGARSATGSIIIAPMNQDDTIADQLKNGVRGFMLDMYDFQNAIWLCHSTQNQCFNFTSFQPAINALKDMRSFLDTNPSEIITIFIEDYVKSP 380
            K+KGLPFNRYSWLT+HNSFA+ G +S TGS+I++P NQ DTI  QL NGVRG MLDMYDFQN IWLCHS   QC+N+T+FQPAIN LK+++ FLD NPSEI+TIFIEDYV SP
Sbjct:   67 KIKGLPFNRYSWLTTHNSFAILGKKSMTGSVILSPTNQQDTITSQLNNGVRGLMLDMYDFQNDIWLCHSFGGQCYNYTAFQPAINVLKEIQVFLDANPSEIVTIFIEDYVTSP 179

HSP 2 Score: 55.0694 bits (131), Expect = 3.857e-55
Identity = 21/31 (67.74%), Postives = 26/31 (83.87%), Query Frame = -2

Query:  379 CDVGLTCQTCPANGNTRPRCSRIQTLNPTTK 471
            CD GL C+TC ANGN RPRC+R+Q +NPT+K
Sbjct:   37 CDSGLHCETCVANGNVRPRCTRVQPINPTSK 67

[Close]

BLAST of GH719623 vs. TrEMBL
Match: D7KF15_ARALY (Phospholipase C OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_474106 PE=4 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 4.158e-54
Identity = 84/127 (66.14%), Postives = 101/127 (79.53%), Query Frame = -3

Query:    6 LPKVKGLPFNRYSWLTSHNSFALAGARSATGSIIIAPMNQDDTIADQLKNGVRGFMLDMYDFQNAIWLCHSTQNQCFNFTSFQPAINALKDMRSFLDTNPSEIITIFIEDYVKSPSGLTKLFQASGI 386
            + K KGLPFN+YSWLT+HNSFA  G  S TGS+I+AP NQ D+I  QL NGVRGFMLDMYDF+N IWLCHS    CFNFT+FQPAIN L++ + FL+ N  E++TI IEDYVKSP GLTK+F A+G+
Sbjct:   67 ISKAKGLPFNKYSWLTTHNSFARLGEVSRTGSVILAPTNQQDSITSQLNNGVRGFMLDMYDFENDIWLCHSFDGTCFNFTAFQPAINILREFQVFLEKNTEEVVTIIIEDYVKSPKGLTKVFDAAGL 193

HSP 2 Score: 48.9062 bits (115), Expect = 4.158e-54
Identity = 20/33 (60.61%), Postives = 25/33 (75.76%), Query Frame = -2

Query:  373 CDVGLTCQTCPANGNTRPRCSRIQTLNPTTKGE 471
            CDVGL C+TC AN + RPRCSR Q +NP +K +
Sbjct:   39 CDVGLHCETCIANTDFRPRCSRTQPINPISKAK 71

[Close]

BLAST of GH719623 vs. TrEMBL
Match: Q9FXA0_ARATH (F14J22.5 protein OS=Arabidopsis thaliana GN=At1g49740 PE=2 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 5.421e-54
Identity = 85/127 (66.93%), Postives = 100/127 (78.74%), Query Frame = -3

Query:    6 LPKVKGLPFNRYSWLTSHNSFALAGARSATGSIIIAPMNQDDTIADQLKNGVRGFMLDMYDFQNAIWLCHSTQNQCFNFTSFQPAINALKDMRSFLDTNPSEIITIFIEDYVKSPSGLTKLFQASGI 386
            + K KGLPFN+YSWLT+HNSFA  G  S TGS I+AP NQ D+I  QL NGVRGFMLDMYDFQN IWLCHS    CFNFT+FQPAIN L++ + FL+ N  E++TI IEDYVKSP GLTK+F A+G+
Sbjct:   67 ITKAKGLPFNKYSWLTTHNSFARLGEVSRTGSAILAPTNQQDSITSQLNNGVRGFMLDMYDFQNDIWLCHSFDGTCFNFTAFQPAINILREFQVFLEKNKEEVVTIIIEDYVKSPKGLTKVFDAAGL 193

HSP 2 Score: 48.9062 bits (115), Expect = 5.421e-54
Identity = 20/33 (60.61%), Postives = 24/33 (72.73%), Query Frame = -2

Query:  373 CDVGLTCQTCPANGNTRPRCSRIQTLNPTTKGE 471
            CD GL C+TC AN + RPRCSR Q +NP TK +
Sbjct:   39 CDAGLHCETCIANTDFRPRCSRTQPINPITKAK 71

[Close]

BLAST of GH719623 vs. TrEMBL
Match: D7L9X8_ARALY (Phospholipase C OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_479446 PE=4 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 9.200e-54
Identity = 84/125 (67.20%), Postives = 103/125 (82.40%), Query Frame = -3

Query:    6 KVKGLPFNRYSWLTSHNSFALAGARSATGSIIIAPMNQDDTIADQLKNGVRGFMLDMYDFQNAIWLCHSTQNQCFNFTSFQPAINALKDMRSFLDTNPSEIITIFIEDYVKSPSGLTKLFQASGI 380
            KVKGLP+N+YSWLT+HNSFA  GA+S TGS+I+AP NQ D+I  QL NGVRGFMLDMYDFQN IWLCHS    CFN+T+FQPA+N LK+ + FLD N   ++T+ +EDYVKSP+GLTK+F ASG+
Sbjct:   68 KVKGLPYNKYSWLTTHNSFARIGAKSGTGSMILAPSNQQDSITSQLLNGVRGFMLDMYDFQNDIWLCHSYGGNCFNYTAFQPAVNILKEFQVFLDKNKDVVVTLILEDYVKSPNGLTKVFDASGL 192

HSP 2 Score: 45.8246 bits (107), Expect = 9.200e-54
Identity = 18/31 (58.06%), Postives = 25/31 (80.65%), Query Frame = -2

Query:  379 CDVGLTCQTCPANGNTRPRCSRIQTLNPTTK 471
            CD GL C++C A+ + RPRCSR+Q +NPT+K
Sbjct:   38 CDRGLHCESCLASDSFRPRCSRMQPINPTSK 68

[Close]

BLAST of GH719623 vs. TrEMBL
Match: Q9LT86_ARATH (MAP3K protein kinase-like protein OS=Arabidopsis thaliana GN=At3g19310 PE=4 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 1.563e-53
Identity = 82/125 (65.60%), Postives = 103/125 (82.40%), Query Frame = -3

Query:    6 KVKGLPFNRYSWLTSHNSFALAGARSATGSIIIAPMNQDDTIADQLKNGVRGFMLDMYDFQNAIWLCHSTQNQCFNFTSFQPAINALKDMRSFLDTNPSEIITIFIEDYVKSPSGLTKLFQASGI 380
            KVKGLP+N+YSWLT+HNSFA  GA+S TGS+I+AP NQ D+I  QL NGVRGFMLD+YDFQN IWLCHS    CFN+T+FQPA+N LK+ + FLD N   ++T+ +EDYVKSP+GLT++F ASG+
Sbjct:   69 KVKGLPYNKYSWLTTHNSFARMGAKSGTGSMILAPSNQQDSITSQLLNGVRGFMLDLYDFQNDIWLCHSYGGNCFNYTAFQPAVNILKEFQVFLDKNKDVVVTLILEDYVKSPNGLTRVFDASGL 193

HSP 2 Score: 47.3654 bits (111), Expect = 1.563e-53
Identity = 19/31 (61.29%), Postives = 25/31 (80.65%), Query Frame = -2

Query:  379 CDVGLTCQTCPANGNTRPRCSRIQTLNPTTK 471
            CD GL C++C A+ + RPRCSR+Q +NPTTK
Sbjct:   39 CDRGLHCESCLASDSFRPRCSRMQPINPTTK 69

[Close]

BLAST of GH719623 vs. TrEMBL
Match: Q84WM6_ARATH (Putative uncharacterized protein At3g19310 (Fragment) OS=Arabidopsis thaliana GN=At3g19310 PE=2 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 1.564e-53
Identity = 82/125 (65.60%), Postives = 103/125 (82.40%), Query Frame = -3

Query:    6 KVKGLPFNRYSWLTSHNSFALAGARSATGSIIIAPMNQDDTIADQLKNGVRGFMLDMYDFQNAIWLCHSTQNQCFNFTSFQPAINALKDMRSFLDTNPSEIITIFIEDYVKSPSGLTKLFQASGI 380
            KVKGLP+N+YSWLT+HNSFA  GA+S TGS+I+AP NQ D+I  QL NGVRGFMLD+YDFQN IWLCHS    CFN+T+FQPA+N LK+ + FLD N   ++T+ +EDYVKSP+GLT++F ASG+
Sbjct:   53 KVKGLPYNKYSWLTTHNSFARMGAKSGTGSMILAPSNQQDSITSQLLNGVRGFMLDLYDFQNDIWLCHSYGGNCFNYTAFQPAVNILKEFQVFLDKNKDVVVTLILEDYVKSPNGLTRVFDASGL 177

HSP 2 Score: 47.3654 bits (111), Expect = 1.564e-53
Identity = 19/31 (61.29%), Postives = 25/31 (80.65%), Query Frame = -2

Query:  379 CDVGLTCQTCPANGNTRPRCSRIQTLNPTTK 471
            CD GL C++C A+ + RPRCSR+Q +NPTTK
Sbjct:   23 CDRGLHCESCLASDSFRPRCSRMQPINPTTK 53

[Close]

BLAST of GH719623 vs. SwissProt
Match: Y5713_ARATH (PI-PLC X domain-containing protein At5g67130 OS=Arabidopsis thaliana GN=At5g67130 PE=1 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 4.389e-43
Identity = 73/125 (58.40%), Postives = 94/125 (75.20%), Query Frame = -3

Query:    3 VKGLPFNRYSWLTSHNSFALAGARSATGSIIIAPMNQDDTIADQLKNGVRGFMLDMYDFQNAIWLCHSTQNQCFNFTSFQPAINALKDMRSFLDTNPSEIITIFIEDYVKSPSGLTKLFQASGIN 377
            + GLPFN+Y+WL +HN+F+ A A    G   I   NQ+DTI +QL+NGVRG MLDMYDF N IWLCHS + QCFNFT+FQPAIN L+++ +FL  NP+EI+TI IEDYV  P GL+ LF  +G++
Sbjct:   77 INGLPFNKYTWLMTHNAFSNANAPLLPGVERITFYNQEDTITNQLQNGVRGLMLDMYDFNNDIWLCHSLRGQCFNFTAFQPAINILREVEAFLSQNPTEIVTIIIEDYVHRPKGLSTLFANAGLD 201

HSP 2 Score: 34.2686 bits (77), Expect = 4.389e-43
Identity = 14/30 (46.67%), Postives = 19/30 (63.33%), Query Frame = -2

Query:  382 CDVGLTCQTCPANGNTRPRCSRIQTLNPTT 471
            C  GL C  CPA G ++P C+R Q  +PT+
Sbjct:   46 CVSGLYCGDCPAVGRSKPVCTRGQATSPTS 75

[Close]

BLAST of GH719623 vs. TAIR peptide
Match: AT4G36945.1 (| Symbols: | PLC-like phosphodiesterases superfamily protein | chr4:17420098-17422047 REVERSE LENGTH=408)

HSP 1 Score: 191.815 bits (486), Expect = 1.460e-59
Identity = 86/126 (68.25%), Postives = 106/126 (84.13%), Query Frame = -3

Query:    3 KVKGLPFNRYSWLTSHNSFALAGARSATGSIIIAPMNQDDTIADQLKNGVRGFMLDMYDFQNAIWLCHSTQNQCFNFTSFQPAINALKDMRSFLDTNPSEIITIFIEDYVKSPSGLTKLFQASGIN 380
            KV GLPFN+YSWLT+HNS+A+ GA SATGS +++P NQ+D+I +QLKNGVRG MLD YDFQN IWLCHST   CFNFT+FQPAINALK++  FL++N SEI+TI +EDYVKS  GLT +F ASG++
Sbjct:   65 KVNGLPFNKYSWLTTHNSYAITGANSATGSFLVSPKNQEDSITNQLKNGVRGIMLDTYDFQNDIWLCHSTGGTCFNFTAFQPAINALKEINDFLESNLSEIVTIILEDYVKSQMGLTNVFNASGLS 190

HSP 2 Score: 55.0694 bits (131), Expect = 1.460e-59
Identity = 22/31 (70.97%), Postives = 26/31 (83.87%), Query Frame = -2

Query:  379 CDVGLTCQTCPANGNTRPRCSRIQTLNPTTK 471
            CD GL+CQ+CPANGNT   C+RIQ LNPT+K
Sbjct:   35 CDAGLSCQSCPANGNTGSTCTRIQPLNPTSK 65

[Close]

BLAST of GH719623 vs. TAIR peptide
Match: AT1G49740.1 (| Symbols: | PLC-like phosphodiesterases superfamily protein | chr1:18407728-18409468 FORWARD LENGTH=359)

HSP 1 Score: 186.422 bits (472), Expect = 4.170e-56
Identity = 85/127 (66.93%), Postives = 100/127 (78.74%), Query Frame = -3

Query:    6 LPKVKGLPFNRYSWLTSHNSFALAGARSATGSIIIAPMNQDDTIADQLKNGVRGFMLDMYDFQNAIWLCHSTQNQCFNFTSFQPAINALKDMRSFLDTNPSEIITIFIEDYVKSPSGLTKLFQASGI 386
            + K KGLPFN+YSWLT+HNSFA  G  S TGS I+AP NQ D+I  QL NGVRGFMLDMYDFQN IWLCHS    CFNFT+FQPAIN L++ + FL+ N  E++TI IEDYVKSP GLTK+F A+G+
Sbjct:   67 ITKAKGLPFNKYSWLTTHNSFARLGEVSRTGSAILAPTNQQDSITSQLNNGVRGFMLDMYDFQNDIWLCHSFDGTCFNFTAFQPAINILREFQVFLEKNKEEVVTIIIEDYVKSPKGLTKVFDAAGL 193

HSP 2 Score: 48.9062 bits (115), Expect = 4.170e-56
Identity = 20/33 (60.61%), Postives = 24/33 (72.73%), Query Frame = -2

Query:  373 CDVGLTCQTCPANGNTRPRCSRIQTLNPTTKGE 471
            CD GL C+TC AN + RPRCSR Q +NP TK +
Sbjct:   39 CDAGLHCETCIANTDFRPRCSRTQPINPITKAK 71

[Close]

BLAST of GH719623 vs. TAIR peptide
Match: AT3G19310.1 (| Symbols: | PLC-like phosphodiesterases superfamily protein | chr3:6694260-6696193 FORWARD LENGTH=413)

HSP 1 Score: 186.422 bits (472), Expect = 1.203e-55
Identity = 82/125 (65.60%), Postives = 103/125 (82.40%), Query Frame = -3

Query:    6 KVKGLPFNRYSWLTSHNSFALAGARSATGSIIIAPMNQDDTIADQLKNGVRGFMLDMYDFQNAIWLCHSTQNQCFNFTSFQPAINALKDMRSFLDTNPSEIITIFIEDYVKSPSGLTKLFQASGI 380
            KVKGLP+N+YSWLT+HNSFA  GA+S TGS+I+AP NQ D+I  QL NGVRGFMLD+YDFQN IWLCHS    CFN+T+FQPA+N LK+ + FLD N   ++T+ +EDYVKSP+GLT++F ASG+
Sbjct:   69 KVKGLPYNKYSWLTTHNSFARMGAKSGTGSMILAPSNQQDSITSQLLNGVRGFMLDLYDFQNDIWLCHSYGGNCFNYTAFQPAVNILKEFQVFLDKNKDVVVTLILEDYVKSPNGLTRVFDASGL 193

HSP 2 Score: 47.3654 bits (111), Expect = 1.203e-55
Identity = 19/31 (61.29%), Postives = 25/31 (80.65%), Query Frame = -2

Query:  379 CDVGLTCQTCPANGNTRPRCSRIQTLNPTTK 471
            CD GL C++C A+ + RPRCSR+Q +NPTTK
Sbjct:   39 CDRGLHCESCLASDSFRPRCSRMQPINPTTK 69

[Close]

BLAST of GH719623 vs. TAIR peptide
Match: AT5G67130.1 (| Symbols: | PLC-like phosphodiesterases superfamily protein | chr5:26791599-26793649 FORWARD LENGTH=426)

HSP 1 Score: 160.614 bits (405), Expect = 5.027e-44
Identity = 73/125 (58.40%), Postives = 94/125 (75.20%), Query Frame = -3

Query:    3 VKGLPFNRYSWLTSHNSFALAGARSATGSIIIAPMNQDDTIADQLKNGVRGFMLDMYDFQNAIWLCHSTQNQCFNFTSFQPAINALKDMRSFLDTNPSEIITIFIEDYVKSPSGLTKLFQASGIN 377
            + GLPFN+Y+WL +HN+F+ A A    G   I   NQ+DTI +QL+NGVRG MLDMYDF N IWLCHS + QCFNFT+FQPAIN L+++ +FL  NP+EI+TI IEDYV  P GL+ LF  +G++
Sbjct:   77 INGLPFNKYTWLMTHNAFSNANAPLLPGVERITFYNQEDTITNQLQNGVRGLMLDMYDFNNDIWLCHSLRGQCFNFTAFQPAINILREVEAFLSQNPTEIVTIIIEDYVHRPKGLSTLFANAGLD 201

HSP 2 Score: 34.2686 bits (77), Expect = 5.027e-44
Identity = 14/30 (46.67%), Postives = 19/30 (63.33%), Query Frame = -2

Query:  382 CDVGLTCQTCPANGNTRPRCSRIQTLNPTT 471
            C  GL C  CPA G ++P C+R Q  +PT+
Sbjct:   46 CVSGLYCGDCPAVGRSKPVCTRGQATSPTS 75

[Close]

BLAST of GH719623 vs. TAIR peptide
Match: AT1G13680.1 (| Symbols: | PLC-like phosphodiesterases superfamily protein | chr1:4690409-4692702 FORWARD LENGTH=346)

HSP 1 Score: 129.798 bits (325), Expect = 6.747e-31
Identity = 59/121 (48.76%), Postives = 82/121 (67.77%), Query Frame = -3

Query:    6 LPFNRYSWLTSHNSFALAGARSATGSIIIAPMNQDDTIADQLKNGVRGFMLDMYDFQNAIWLCHSTQNQCFNFTSFQPAINALKDMRSFLDTNPSEIITIFIEDYVKSPSGLTKLFQASGI 368
            +PFN+Y++LT+HNS+A+ G      +       Q+DTI  QL +GVR  MLD YD++  +W CHS   QCF FT F  AI+  K++ +FL  NPSEI+T+ +EDYVKS +GLTK+F  SG+
Sbjct:   74 MPFNKYAFLTTHNSYAIEGKALHVAT-------QEDTIVQQLNSGVRALMLDTYDYEGDVWFCHSFDEQCFEFTKFNRAIDTFKEIFAFLTANPSEIVTLILEDYVKSQNGLTKVFTDSGL 187

The following BLAST results are available for this feature:

BLAST of GH719623 vs. TrEMBL
BLAST of GH719623 vs. SwissProt
BLAST of GH719623 vs. TAIR peptide

BLAST of GH719623 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7MAZ7_ARALY	4.601e-59	70.63	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
B9N4Z2_POPTR	5.043e-58	72.00	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
O23190_ARATH	1.887e-57	68.25	MAP3K-like protein kinase OS=Arabidopsis thaliana ...	[more]
B9H6H1_POPTR	2.069e-56	69.60	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
C6TH52_SOYBN	3.857e-55	72.57	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7KF15_ARALY	4.158e-54	66.14	Phospholipase C OS=Arabidopsis lyrata subsp. lyrat...	[more]
Q9FXA0_ARATH	5.421e-54	66.93	F14J22.5 protein OS=Arabidopsis thaliana GN=At1g49...	[more]
D7L9X8_ARALY	9.200e-54	67.20	Phospholipase C OS=Arabidopsis lyrata subsp. lyrat...	[more]
Q9LT86_ARATH	1.563e-53	65.60	MAP3K protein kinase-like protein OS=Arabidopsis t...	[more]
Q84WM6_ARATH	1.564e-53	65.60	Putative uncharacterized protein At3g19310 (Fragme...	[more]

BLAST of GH719623 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 1


Match Name	E-value	Identity	Description
Y5713_ARATH	4.389e-43	58.40	PI-PLC X domain-containing protein At5g67130 OS=Ar...	[more]

BLAST of GH719623 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT4G36945.1	1.460e-59	68.25	\| Symbols: \| PLC-like phosphodiesterases superfam...	[more]
AT1G49740.1	4.170e-56	66.93	\| Symbols: \| PLC-like phosphodiesterases superfam...	[more]
AT3G19310.1	1.203e-55	65.60	\| Symbols: \| PLC-like phosphodiesterases superfam...	[more]
AT5G67130.1	5.027e-44	58.40	\| Symbols: \| PLC-like phosphodiesterases superfam...	[more]
AT1G13680.1	6.747e-31	48.76	\| Symbols: \| PLC-like phosphodiesterases superfam...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000909	Phospholipase C, phosphatidylinositol-specific , X domain	PROFILE	PS50007	PIPLC_X_DOMAIN	coord: 10..117 score: 9
IPR017946	PLC-like phosphodiesterase, TIM beta/alpha-barrel domain	GENE3D	3.20.20.190	no description	coord: 2..124 score: 4
IPR017946	PLC-like phosphodiesterase, TIM beta/alpha-barrel domain	SUPERFAMILY	51695	PLC-like phosphodiesterases	coord: 8..125 score: 1.3

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH719623 ID=GH719623; Name=GH719623; organism=Pisum sativum; type=EST; length=472bp
TTGTTTATTCCGGAAGCTTGGAAGAGCTTAGTTAGGCCTGAAGGAGATTT
GACATAATCCTCGATGAAGATGGTGATTATTTCAGATGGATTTGTGTCTA
GAAACGAACGCATGTCTTTAAGCGCATTTATTGCTGGTTGAAAGGAGGTG
AAGTTGAAACATTGGTTTTGAGTAGAATGACACAACCATATGGCATTTTG
AAAATCATACATATCTAACATAAACCCTCGAACACCATTTTTAAGTTGAT
CAGCAATCGTGTCATCCTGGTTCATAGGAGCAATGATAATGGATCCTGTG
GCCGATCTTGCCCCAGCTAAAGCAAACGAATTATGCGACGTTAACCATGA
ATACCGATTAAACGGTAAACCTTTCACCTTTGGTAGTTGGGTTTAAAGTT
TGTATTCTGCTACATCTGGGTCTTGTATTCCCATTCGCAGGACATGTTTG
ACATGTAAGACCAACATCACAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004629	phospholipase C activity
GO:0008081	phosphoric diester hydrolase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006629	lipid metabolic process
GO:0023034	intracellular signaling pathway

Vocabulary: **INTERPRO**
Term	Definition
IPR000909	PLipase_C_PInositol-sp_X_dom
IPR017946	PLC-like_Pdiesterase_TIM-brl

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