GH720304

EST Overview


Name	GH720304
Unique Name	GH720304
Type	EST
Organism	Pisum sativum (pea)
Sequence length	565

Libraries


Library Name	Type
LIBEST_024270 552I	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of GH720304 vs. TrEMBL
Match: B3IX28_LOTJA (Transcription factor CCAAT OS=Lotus japonicus GN=CBF-A22 PE=2 SV=1)

HSP 1 Score: 216.853 bits (551), Expect = 7.738e-55
Identity = 108/159 (67.92%), Postives = 123/159 (77.36%), Query Frame = -1

Query:   74 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNTXXXXXXXXXXXXXXXKFCGQQLQCSGSPSSELFESDVGTLNSSKETNGSSPNISGFEVTSSYSRGNLGSFSVNHLGPTVHSLADMIDGGRGVIMPTKWVTAAGNCCNL 550
            +YHGIIRRRQSRAKAVL +K+ KRRKPY+HESRHLHA+RRPRGCGGRFLNT               K  G+QLQ SGS  S++F+S+VGTLNSSKETNGS PN+SG EVTS YSRG   +FS NHLG +V+ LADM+D GRG IMPTKWV A GN CNL
Sbjct:  169 QYHGIIRRRQSRAKAVLDRKMTKRRKPYLHESRHLHALRRPRGCGGRFLNTKVSANGNGKTGRDVKKTGGEQLQSSGSQGSDVFQSEVGTLNSSKETNGSGPNVSGLEVTSMYSRGGFDTFSFNHLGSSVYPLADMVDCGRG-IMPTKWVAAGGNSCNL 326

[Close]

BLAST of GH720304 vs. TrEMBL
Match: B3IX33_LOTJA (Transcription factor CCAAT OS=Lotus japonicus GN=CBF-A01 PE=2 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 2.489e-53
Identity = 112/164 (68.29%), Postives = 122/164 (74.39%), Query Frame = -1

Query:   71 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNT---XXXXXXXXXXXXXXXKFCGQQLQCSGSPSSELFESDVGTLNSSKETNGSSPNISGFEVTSSYSRGNLGSFSVNHLGPTV-HSLADMIDGGRGVIMPTKWVTAAGNCCNLK 550
            +YHGIIRRRQSRAKAV   KLIKR KPYMHESRHLHAMRRPRGCGGRFLNT                     G+ +Q S S SSE+ +S+VGTLNSSKETNGSSPNISG EVTS  S G L SF+VNHLG  + HSLADMIDGG G+IMPTKWV AAG+C NLK
Sbjct:  168 QYHGIIRRRQSRAKAVQENKLIKRSKPYMHESRHLHAMRRPRGCGGRFLNTRNSSDGNGKSGSELHKKTGGLGRHMQSSASQSSEVLQSEVGTLNSSKETNGSSPNISGSEVTSMCSLGGLDSFAVNHLGSAIHHSLADMIDGGHGIIMPTKWVGAAGSCFNLK 331

[Close]

BLAST of GH720304 vs. TrEMBL
Match: A4ZVU9_MEDTR (CCAAT-binding transcription factor OS=Medicago truncatula GN=HAP2.1 PE=4 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 1.614e-52
Identity = 106/155 (68.39%), Postives = 117/155 (75.48%), Query Frame = -1

Query:   89 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNTXXXXXXXXXXXXXXXK-FCGQQLQCSGSPSSELFESDVGTLNSSKETNGSSPNISGFEVTSSYSRGNLGSFSVNHLGPTVHSLADMIDGGRGVIMPTKWVTAAG 550
            +YHGIIRRR SRAKAVL  KLIKR KPYMHESRHLHAMRRPRGCGGRFLNT                   G QLQ SGS SSE+ +S+VGTLNSSKETNG SPN+SG EVTS Y++G L SF+VNH+G TVHSL DMID G G++MPTKW  AAG
Sbjct:  173 QYHGIIRRRHSRAKAVLQNKLIKRNKPYMHESRHLHAMRRPRGCGGRFLNTKVSANGNGKSGSEENGNIGGLQLQSSGSQSSEVLQSEVGTLNSSKETNGGSPNVSGSEVTSMYTQGVLDSFTVNHIGSTVHSLGDMIDTGHGIVMPTKWFAAAG 327

[Close]

BLAST of GH720304 vs. TrEMBL
Match: B9IIJ3_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_576480 PE=4 SV=1)

HSP 1 Score: 177.563 bits (449), Expect = 5.203e-43
Identity = 91/160 (56.88%), Postives = 104/160 (65.00%), Query Frame = -1

Query:   71 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNTXXXXXXXXXXXXXXXKFCGQQLQCSGSPSSELFESDVGTLNSSKETNGSSPNISGFEVTSSYSRGNLGSFSVNHLGPTVHSLADMIDGGRGVIMPTKWVTAAGNCCNLK 550
            +YHGI+RRR+SRAKAVL  K    RKPYMH SRHLHAMRRPRGCGGRFLNT                   Q  Q +GS +SE+ ES   TLNSS E NG     SG EVTS Y+RG L    +N LGPT HS + M+D G G++MP+KWV AA NCCNLK
Sbjct:  115 QYHGIMRRRKSRAKAVLENKSTNSRKPYMHYSRHLHAMRRPRGCGGRFLNTKELNEGKGTMEAKKAGDF-QPSQATGSQNSEVLESGGATLNSSMEANGGGSIFSGSEVTSMYNRGELDLLQINRLGPTFHSFSGMMDRGHGIVMPSKWVAAADNCCNLK 273

[Close]

BLAST of GH720304 vs. TrEMBL
Match: B9SDK4_RICCO (Transcription factor, putative OS=Ricinus communis GN=RCOM_0420390 PE=4 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 8.596e-38
Identity = 85/160 (53.12%), Postives = 105/160 (65.62%), Query Frame = -1

Query:   71 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNTXXXXXXXXXXXXXXXKFCGQQLQCSGSPSSELFESDVGTLNSSKETNGSSPNISGFEVTSSYSRGNLGSFSVNHLGPTVHSLADMIDGGRGVIMPTKWVTAAGNCCNLK 550
            +YHGIIRRR+SRAKA L  + I++RKPYMH SRHLHAMRRPRG GGRFLN+               +      Q +GS SSE+ +SD G       TNG   N+SG EVTS YS+ +L  FS+N LGP VHS + M+D G  ++MP+KWV AA +CCNLK
Sbjct:  164 QYHGIIRRRKSRAKAELENRPIRKRKPYMHLSRHLHAMRRPRGTGGRFLNSGNGKGGAVAKKAYDVEIS----QPTGSHSSEVLQSDSG------GTNGGGSNLSGSEVTSMYSKRDLDHFSINLLGPPVHSFSVMMDNGHSIVMPSKWVAAAEDCCNLK 313

[Close]

BLAST of GH720304 vs. TrEMBL
Match: B9H9L4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_561162 PE=4 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 5.572e-37
Identity = 89/161 (55.28%), Postives = 101/161 (62.73%), Query Frame = -1

Query:   71 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNTXXXXXXXXXXXXXXXKFCGQQLQCSGSPSSELFESDVGTLNSSKETNGSSPNISGFEVTSSYSRGNLGSFSVNHLGPTVHSLADMIDG-GRGVIMPTKWVTAAGNCCNLK 550
            +YHGIIRRR+SRAKA L  KL + RKPYMH SRHLHAMRRPRGCGGRFLNT                   Q  Q +GS SSE+ ES   TLN S E N    N+SG EVTS Y+R +   F  NH GPTVH  + M  G G G+ MP+KW+ AA NC NLK
Sbjct:  123 QYHGIIRRRKSRAKAALENKLPRNRKPYMHRSRHLHAMRRPRGCGGRFLNTKELNDGKGITEAKKAGDF-QLSQPTGSQSSEVLESGGATLN-SMEANCGGSNLSGSEVTSLYNRVDFDRFPFNHHGPTVHGFSGMDGGHGIGIGMPSKWIAAADNCSNLK 281

[Close]

BLAST of GH720304 vs. TrEMBL
Match: A5PGU6_PETHY (YA1 (Fragment) OS=Petunia hybrida GN=nf-YA PE=2 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 2.341e-35
Identity = 81/160 (50.62%), Postives = 104/160 (65.00%), Query Frame = -1

Query:   71 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNTXXXXXXXXXXXXXXXKFCGQQLQCSGSPSSELFESDVGTLNSSKETNGSSPNISGFEVTSSYSRGNLGSFSVNHLGPTVHSLADMIDGGRGVIMPTKWVTAAGNCCNLK 550
            +YHGI+RRR+SRAK  + +K +K RKPY+H SRHLHAMRRPRGCGGRFLNT                 C Q    +GS +SE+ +SD   L+S KET GS       EVT+ YSRGNL  F   +L P+V ++ DM++ G G++M  KWV+AA +CCNLK
Sbjct:    2 QYHGILRRRKSRAKE-MEKKALKPRKPYLHLSRHLHAMRRPRGCGGRFLNTKSMKGSMKGGKTNDTGEC-QYFYPTGSQNSEVLQSDSSNLSSPKETTGSR-FCDSSEVTNMYSRGNLDPFPFQNLRPSVQAIPDMMNTGHGILMAGKWVSAADSCCNLK 158

[Close]

BLAST of GH720304 vs. TrEMBL
Match: D7U8S9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_32.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00022601001 PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 3.498e-31
Identity = 80/161 (49.69%), Postives = 91/161 (56.52%), Query Frame = -1

Query:   68 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNTXXXXXXXXXXXXXXXKFCGQQLQCSGSPSSELFESDVGTLNSSKETNGSSPNISGFEVTSSYSRGNLGSFSVNHLGPTVHSLADMIDGGRGVIMPTKWVTAAGNCCNLKA 550
            +YHGIIRRRQSRAKA L  KL + RKPYMHESRHLHAMRRPRGCGGRFL                     Q  Q +GS  SE+ +SD G +NS KE NG+   + G EVTS YSR  L                     G G++MP+KWV AA N CN KA
Sbjct:  170 QYHGIIRRRQSRAKAELENKLTRARKPYMHESRHLHAMRRPRGCGGRFLKKNLNGGKCGTDMKKVDN--RQLSQPTGSQISEVLQSDGGNMNSPKEANGNLSKLLGSEVTSMYSRRYLHQ-------------------GHGIVMPSKWVAAADNRCNPKA 309

[Close]

BLAST of GH720304 vs. TrEMBL
Match: C5YSE1_SORBI (Putative uncharacterized protein Sb08g021910 OS=Sorghum bicolor GN=Sb08g021910 PE=4 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.205e-23
Identity = 67/162 (41.36%), Postives = 92/162 (56.79%), Query Frame = -1

Query:   74 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNTXXXXXXXXXXXXXXXKFCGQQLQCSGSPSSELFESDVGTLNSSKETNGSSPNISGFEVTSSYSRGNLGSF-SVNHL-GPTVHSLADMIDGGRGVI-MPTKWVTAAGNCCNL 550
            +Y  I+RRR++RAKA    +L+K RKPY+HESRH HA+RRPRG GGRFLNT                F    ++   SPSSE+  SD+G  +S         ++SG EV+S Y R ++  + S +HL  P    L  ++DG  GV+  P KW  A+  CC+L
Sbjct:   47 QYDAIMRRRRARAKAERENRLVKARKPYLHESRHQHALRRPRGSGGRFLNTKKESDGKDAGGGSKATFSNPLMRQVASPSSEIQHSDLGNPSSVS-------SLSGSEVSSMYDREDMDHYHSFDHLRTPFFTPLTSIMDGDHGVVGNPFKWAAASEVCCDL 201

[Close]

BLAST of GH720304 vs. TrEMBL
Match: D7LZR1_ARALY (CCAAT-binding transcription factor (CBF-B/NF-YA) family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_908579 PE=4 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 2.055e-23
Identity = 74/161 (45.96%), Postives = 90/161 (55.90%), Query Frame = -1

Query:   74 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNTXXXXXXXXXXXXXXXKFCGQQLQCSGSPSSELFESDVGTLNSSKETNGSSPNISGFEVTSSYSRGNLGSFSVNHLGPTVHSLADMIDGGRGVIMPTKWVTAAG--NCCNL 550
            +YHGIIRRRQSRAKA   +KL + RKPYMH SRHLHAMRRPRG GGRFLNT                   +Q + S S SSE+F  +  T+NSS+E NGS  N+S   VT      N+  F           L+  +    G++MP KW  AA   +CCNL
Sbjct:  142 QYHGIIRRRQSRAKA---EKLSRCRKPYMHHSRHLHAMRRPRGSGGRFLNTKTAD-------------AAKQSKPSNSQSSEVFHPENRTINSSREANGS--NLSDSTVT------NMDYF-----------LSSSVYSSGGMVMPIKWNAAAMDISCCNL 267

[Close]

BLAST of GH720304 vs. SwissProt
Match: NFYA2_ARATH (Nuclear transcription factor Y subunit A-2 OS=Arabidopsis thaliana GN=NFYA2 PE=2 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 1.866e-22
Identity = 78/174 (44.83%), Postives = 93/174 (53.45%), Query Frame = -1

Query:   71 RYHGIIRRRQSRAKA--VLGQKLIKRR--KPYMHESRHLHAMRRPRGCGGRFLNTXXXXXXXXXXXXXXXKFCGQ-----QLQCSGSPSSELFESDVGTLNSSKETNGSSPNISGFEVTSSYSRGNLGSFSVNH-LGPTVHSLADMIDGGRGVIMPTKWVTAA----GNCCNLK 550
            +YHGIIRRRQSRAKA  VL QK +  R  KPYMH SRHLHA+RRPRG GGRFLNT                   Q     + Q S S +SE+   + GT+N S   NG   N+SG EVT           S+N+ L   VHSL        G++MP+KW+ AA      CCN K
Sbjct:  144 QYHGIIRRRQSRAKAAAVLDQKKLSSRCRKPYMHHSRHLHALRRPRGSGGRFLNTKSQNLENSGTNAKKGDGSMQIQSQPKPQQSNSQNSEVVHPENGTMNLS---NGL--NVSGSEVT-----------SMNYFLSSPVHSLG-------GMVMPSKWIAAAAAMDNGCCNFK 294

[Close]

BLAST of GH720304 vs. SwissProt
Match: NFYAA_ARATH (Nuclear transcription factor Y subunit A-10 OS=Arabidopsis thaliana GN=NFYA10 PE=2 SV=2)

HSP 1 Score: 104.76 bits (260), Expect = 3.183e-22
Identity = 71/161 (44.10%), Postives = 86/161 (53.42%), Query Frame = -1

Query:   74 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNTXXXXXXXXXXXXXXXKFCGQQLQCSGSPSSELFESDVGTLNSSKETNGSSPNISGFEVTSSYSRGNLGSFSVNHLGPTVHSLADMIDGGRGVIMPTKWVTAAGN--CCNL 550
            +YHGIIRRRQSRAKA   +KL + RKPYMH SRHLHAMRRPRG GGRFLNT                   +Q + S S SSE+F  +  T+NSS+E N S  N+S   VTS                   + L+       G++MP KW  AA +  CC L
Sbjct:  142 QYHGIIRRRQSRAKA---EKLSRCRKPYMHHSRHLHAMRRPRGSGGRFLNTKTAD-------------AAKQSKPSNSQSSEVFHPENETINSSREANES--NLSDSAVTS-----------------MDYFLSSSAYSPGGMVMPIKWNAAAMDIGCCKL 267

[Close]

BLAST of GH720304 vs. SwissProt
Match: NFYA7_ARATH (Nuclear transcription factor Y subunit A-7 OS=Arabidopsis thaliana GN=NFYA7 PE=2 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 8.969e-17
Identity = 38/50 (76.00%), Postives = 44/50 (88.00%), Query Frame = -1

Query:  401 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLN 550
            +YHGI+RRRQSRA+     K+IK RKPY+HESRHLHA+RRPRGCGGRFLN
Sbjct:  108 QYHGILRRRQSRARLESQNKVIKSRKPYLHESRHLHAIRRPRGCGGRFLN 157

[Close]

BLAST of GH720304 vs. SwissProt
Match: NFYA4_ARATH (Nuclear transcription factor Y subunit A-4 OS=Arabidopsis thaliana GN=NFYA4 PE=2 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.171e-16
Identity = 38/50 (76.00%), Postives = 43/50 (86.00%), Query Frame = -1

Query:  401 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLN 550
            +YHGI+RRRQSRAK     + IK +KPYMHESRHLHA+RRPRGCGGRFLN
Sbjct:  106 QYHGILRRRQSRAKLEARNRAIKAKKPYMHESRHLHAIRRPRGCGGRFLN 155

[Close]

BLAST of GH720304 vs. SwissProt
Match: NFYA3_ARATH (Nuclear transcription factor Y subunit A-3 OS=Arabidopsis thaliana GN=NFYA3 PE=2 SV=2)

HSP 1 Score: 80.1073 bits (196), Expect = 8.395e-15
Identity = 35/51 (68.63%), Postives = 42/51 (82.35%), Query Frame = -1

Query:  398 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNT 550
            +YH I+RRRQ RAK     KLI+ RKPY+HESRH+HA++RPRG GGRFLNT
Sbjct:  187 QYHAIMRRRQQRAKLEAQNKLIRARKPYLHESRHVHALKRPRGSGGRFLNT 237

[Close]

BLAST of GH720304 vs. SwissProt
Match: NFYA8_ARATH (Nuclear transcription factor Y subunit A-8 OS=Arabidopsis thaliana GN=NFYA8 PE=2 SV=2)

HSP 1 Score: 79.7221 bits (195), Expect = 1.096e-14
Identity = 35/51 (68.63%), Postives = 42/51 (82.35%), Query Frame = -1

Query:  398 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNT 550
            ++H I+RRRQ RAK     KLIK RKPY+HESRH+HA++RPRG GGRFLNT
Sbjct:  180 QFHAIMRRRQQRAKLEAQNKLIKARKPYLHESRHVHALKRPRGSGGRFLNT 230

[Close]

BLAST of GH720304 vs. SwissProt
Match: NFYA9_ARATH (Nuclear transcription factor Y subunit A-9 OS=Arabidopsis thaliana GN=NFYA9 PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 2.442e-14
Identity = 36/48 (75.00%), Postives = 41/48 (85.42%), Query Frame = -1

Query:  407 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRF 550
            +Y  I+RRRQ+RAKA L +KLIK RKPY+HESRH HAMRRPRG GGRF
Sbjct:  174 QYQAILRRRQARAKAELEKKLIKSRKPYLHESRHQHAMRRPRGTGGRF 221

[Close]

BLAST of GH720304 vs. SwissProt
Match: NFYA5_ARATH (Nuclear transcription factor Y subunit A-5 OS=Arabidopsis thaliana GN=NFYA5 PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 7.107e-14
Identity = 35/51 (68.63%), Postives = 41/51 (80.39%), Query Frame = -1

Query:  398 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNT 550
            +YH I+RRR+ RAK     KLIK RKPY+HESRHLHA++R RG GGRFLNT
Sbjct:  186 QYHAILRRRKHRAKLEAQNKLIKCRKPYLHESRHLHALKRARGSGGRFLNT 236

[Close]

BLAST of GH720304 vs. SwissProt
Match: NFYA6_ARATH (Nuclear transcription factor Y subunit A-6 OS=Arabidopsis thaliana GN=NFYA6 PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 6.016e-13
Identity = 34/51 (66.67%), Postives = 40/51 (78.43%), Query Frame = -1

Query:  398 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNT 550
            +Y  I+RRR+ RAK     KLIK RKPY+HESRHLHA++R RG GGRFLNT
Sbjct:  179 QYQAILRRRERRAKLEAQNKLIKVRKPYLHESRHLHALKRVRGSGGRFLNT 229

[Close]

BLAST of GH720304 vs. SwissProt
Match: NFYA1_ARATH (Nuclear transcription factor Y subunit A-1 OS=Arabidopsis thaliana GN=NFYA1 PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 3.899e-12
Identity = 32/48 (66.67%), Postives = 40/48 (83.33%), Query Frame = -1

Query:  407 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRF 550
            +Y GI+RRR++RAKA L +K+I+ RKPY+HESRH HAMRR R  GGRF
Sbjct:  180 QYEGILRRRKARAKAELERKVIRDRKPYLHESRHKHAMRRARASGGRF 227

[Close]

BLAST of GH720304 vs. TAIR peptide
Match: AT3G05690.1 (| Symbols: UNE8, ATHAP2B, HAP2B, NF-YA2 | nuclear factor Y, subunit A2 | chr3:1676922-1678324 REVERSE LENGTH=295)

HSP 1 Score: 105.531 bits (262), Expect = 1.989e-23
Identity = 78/174 (44.83%), Postives = 93/174 (53.45%), Query Frame = -1

Query:   71 RYHGIIRRRQSRAKA--VLGQKLIKRR--KPYMHESRHLHAMRRPRGCGGRFLNTXXXXXXXXXXXXXXXKFCGQ-----QLQCSGSPSSELFESDVGTLNSSKETNGSSPNISGFEVTSSYSRGNLGSFSVNH-LGPTVHSLADMIDGGRGVIMPTKWVTAA----GNCCNLK 550
            +YHGIIRRRQSRAKA  VL QK +  R  KPYMH SRHLHA+RRPRG GGRFLNT                   Q     + Q S S +SE+   + GT+N S   NG   N+SG EVT           S+N+ L   VHSL        G++MP+KW+ AA      CCN K
Sbjct:  144 QYHGIIRRRQSRAKAAAVLDQKKLSSRCRKPYMHHSRHLHALRRPRGSGGRFLNTKSQNLENSGTNAKKGDGSMQIQSQPKPQQSNSQNSEVVHPENGTMNLS---NGL--NVSGSEVT-----------SMNYFLSSPVHSLG-------GMVMPSKWIAAAAAMDNGCCNFK 294

[Close]

BLAST of GH720304 vs. TAIR peptide
Match: AT5G06510.2 (| Symbols: NF-YA10 | nuclear factor Y, subunit A10 | chr5:1985439-1986591 FORWARD LENGTH=269)

HSP 1 Score: 104.76 bits (260), Expect = 3.392e-23
Identity = 71/161 (44.10%), Postives = 86/161 (53.42%), Query Frame = -1

Query:   74 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNTXXXXXXXXXXXXXXXKFCGQQLQCSGSPSSELFESDVGTLNSSKETNGSSPNISGFEVTSSYSRGNLGSFSVNHLGPTVHSLADMIDGGRGVIMPTKWVTAAGN--CCNL 550
            +YHGIIRRRQSRAKA   +KL + RKPYMH SRHLHAMRRPRG GGRFLNT                   +Q + S S SSE+F  +  T+NSS+E N S  N+S   VTS                   + L+       G++MP KW  AA +  CC L
Sbjct:  142 QYHGIIRRRQSRAKA---EKLSRCRKPYMHHSRHLHAMRRPRGSGGRFLNTKTAD-------------AAKQSKPSNSQSSEVFHPENETINSSREANES--NLSDSAVTS-----------------MDYFLSSSAYSPGGMVMPIKWNAAAMDIGCCKL 267

[Close]

BLAST of GH720304 vs. TAIR peptide
Match: AT5G06510.3 (| Symbols: NF-YA10 | nuclear factor Y, subunit A10 | chr5:1985758-1986591 FORWARD LENGTH=220)

HSP 1 Score: 104.76 bits (260), Expect = 3.392e-23
Identity = 71/161 (44.10%), Postives = 86/161 (53.42%), Query Frame = -1

Query:   74 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNTXXXXXXXXXXXXXXXKFCGQQLQCSGSPSSELFESDVGTLNSSKETNGSSPNISGFEVTSSYSRGNLGSFSVNHLGPTVHSLADMIDGGRGVIMPTKWVTAAGN--CCNL 550
            +YHGIIRRRQSRAKA   +KL + RKPYMH SRHLHAMRRPRG GGRFLNT                   +Q + S S SSE+F  +  T+NSS+E N S  N+S   VTS                   + L+       G++MP KW  AA +  CC L
Sbjct:   93 QYHGIIRRRQSRAKA---EKLSRCRKPYMHHSRHLHAMRRPRGSGGRFLNTKTAD-------------AAKQSKPSNSQSSEVFHPENETINSSREANES--NLSDSAVTS-----------------MDYFLSSSAYSPGGMVMPIKWNAAAMDIGCCKL 218

[Close]

BLAST of GH720304 vs. TAIR peptide
Match: AT5G06510.1 (| Symbols: NF-YA10 | nuclear factor Y, subunit A10 | chr5:1985439-1986591 FORWARD LENGTH=269)

HSP 1 Score: 104.76 bits (260), Expect = 3.392e-23
Identity = 71/161 (44.10%), Postives = 86/161 (53.42%), Query Frame = -1

Query:   74 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNTXXXXXXXXXXXXXXXKFCGQQLQCSGSPSSELFESDVGTLNSSKETNGSSPNISGFEVTSSYSRGNLGSFSVNHLGPTVHSLADMIDGGRGVIMPTKWVTAAGN--CCNL 550
            +YHGIIRRRQSRAKA   +KL + RKPYMH SRHLHAMRRPRG GGRFLNT                   +Q + S S SSE+F  +  T+NSS+E N S  N+S   VTS                   + L+       G++MP KW  AA +  CC L
Sbjct:  142 QYHGIIRRRQSRAKA---EKLSRCRKPYMHHSRHLHAMRRPRGSGGRFLNTKTAD-------------AAKQSKPSNSQSSEVFHPENETINSSREANES--NLSDSAVTS-----------------MDYFLSSSAYSPGGMVMPIKWNAAAMDIGCCKL 267

[Close]

BLAST of GH720304 vs. TAIR peptide
Match: AT1G30500.2 (| Symbols: NF-YA7 | nuclear factor Y, subunit A7 | chr1:10804736-10805896 REVERSE LENGTH=190)

HSP 1 Score: 86.6557 bits (213), Expect = 9.560e-18
Identity = 38/50 (76.00%), Postives = 44/50 (88.00%), Query Frame = -1

Query:  401 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLN 550
            +YHGI+RRRQSRA+     K+IK RKPY+HESRHLHA+RRPRGCGGRFLN
Sbjct:  108 QYHGILRRRQSRARLESQNKVIKSRKPYLHESRHLHAIRRPRGCGGRFLN 157

[Close]

BLAST of GH720304 vs. TAIR peptide
Match: AT1G30500.1 (| Symbols: NF-YA7 | nuclear factor Y, subunit A7 | chr1:10804736-10805896 REVERSE LENGTH=186)

HSP 1 Score: 86.6557 bits (213), Expect = 9.560e-18
Identity = 38/50 (76.00%), Postives = 44/50 (88.00%), Query Frame = -1

Query:  401 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLN 550
            +YHGI+RRRQSRA+     K+IK RKPY+HESRHLHA+RRPRGCGGRFLN
Sbjct:  104 QYHGILRRRQSRARLESQNKVIKSRKPYLHESRHLHAIRRPRGCGGRFLN 153

[Close]

BLAST of GH720304 vs. TAIR peptide
Match: AT2G34720.1 (| Symbols: NF-YA4 | nuclear factor Y, subunit A4 | chr2:14650018-14651255 REVERSE LENGTH=198)

HSP 1 Score: 86.2705 bits (212), Expect = 1.249e-17
Identity = 38/50 (76.00%), Postives = 43/50 (86.00%), Query Frame = -1

Query:  401 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLN 550
            +YHGI+RRRQSRAK     + IK +KPYMHESRHLHA+RRPRGCGGRFLN
Sbjct:  106 QYHGILRRRQSRAKLEARNRAIKAKKPYMHESRHLHAIRRPRGCGGRFLN 155

[Close]

BLAST of GH720304 vs. TAIR peptide
Match: AT1G72830.3 (| Symbols: HAP2C, ATHAP2C, NF-YA3 | nuclear factor Y, subunit A3 | chr1:27405699-27407088 REVERSE LENGTH=315)

HSP 1 Score: 80.1073 bits (196), Expect = 8.947e-16
Identity = 35/51 (68.63%), Postives = 42/51 (82.35%), Query Frame = -1

Query:  398 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNT 550
            +YH I+RRRQ RAK     KLI+ RKPY+HESRH+HA++RPRG GGRFLNT
Sbjct:  162 QYHAIMRRRQQRAKLEAQNKLIRARKPYLHESRHVHALKRPRGSGGRFLNT 212

[Close]

BLAST of GH720304 vs. TAIR peptide
Match: AT1G72830.1 (| Symbols: HAP2C, ATHAP2C, NF-YA3 | nuclear factor Y, subunit A3 | chr1:27405699-27407088 REVERSE LENGTH=340)

HSP 1 Score: 80.1073 bits (196), Expect = 8.947e-16
Identity = 35/51 (68.63%), Postives = 42/51 (82.35%), Query Frame = -1

Query:  398 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNT 550
            +YH I+RRRQ RAK     KLI+ RKPY+HESRH+HA++RPRG GGRFLNT
Sbjct:  187 QYHAIMRRRQQRAKLEAQNKLIRARKPYLHESRHVHALKRPRGSGGRFLNT 237

[Close]

BLAST of GH720304 vs. TAIR peptide
Match: AT1G17590.4 (| Symbols: NF-YA8 | nuclear factor Y, subunit A8 | chr1:6050481-6051984 REVERSE LENGTH=328)

HSP 1 Score: 79.7221 bits (195), Expect = 1.169e-15
Identity = 35/51 (68.63%), Postives = 42/51 (82.35%), Query Frame = -1

Query:  398 RYHGIIRRRQSRAKAVLGQKLIKRRKPYMHESRHLHAMRRPRGCGGRFLNT 550
            ++H I+RRRQ RAK     KLIK RKPY+HESRH+HA++RPRG GGRFLNT
Sbjct:  180 QFHAIMRRRQQRAKLEAQNKLIKARKPYLHESRHVHALKRPRGSGGRFLNT 230

The following BLAST results are available for this feature:

BLAST of GH720304 vs. TrEMBL
BLAST of GH720304 vs. SwissProt
BLAST of GH720304 vs. TAIR peptide

BLAST of GH720304 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B3IX28_LOTJA	7.738e-55	67.92	Transcription factor CCAAT OS=Lotus japonicus GN=C...	[more]
B3IX33_LOTJA	2.489e-53	68.29	Transcription factor CCAAT OS=Lotus japonicus GN=C...	[more]
A4ZVU9_MEDTR	1.614e-52	68.39	CCAAT-binding transcription factor OS=Medicago tru...	[more]
B9IIJ3_POPTR	5.203e-43	56.88	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SDK4_RICCO	8.596e-38	53.13	Transcription factor, putative OS=Ricinus communis...	[more]
B9H9L4_POPTR	5.572e-37	55.28	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A5PGU6_PETHY	2.341e-35	50.63	YA1 (Fragment) OS=Petunia hybrida GN=nf-YA PE=2 SV...	[more]
D7U8S9_VITVI	3.498e-31	49.69	Whole genome shotgun sequence of line PN40024, sca...	[more]
C5YSE1_SORBI	1.205e-23	41.36	Putative uncharacterized protein Sb08g021910 OS=So...	[more]
D7LZR1_ARALY	2.055e-23	45.96	CCAAT-binding transcription factor (CBF-B/NF-YA) f...	[more]

BLAST of GH720304 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
NFYA2_ARATH	1.866e-22	44.83	Nuclear transcription factor Y subunit A-2 OS=Arab...	[more]
NFYAA_ARATH	3.183e-22	44.10	Nuclear transcription factor Y subunit A-10 OS=Ara...	[more]
NFYA7_ARATH	8.969e-17	76.00	Nuclear transcription factor Y subunit A-7 OS=Arab...	[more]
NFYA4_ARATH	1.171e-16	76.00	Nuclear transcription factor Y subunit A-4 OS=Arab...	[more]
NFYA3_ARATH	8.395e-15	68.63	Nuclear transcription factor Y subunit A-3 OS=Arab...	[more]
NFYA8_ARATH	1.096e-14	68.63	Nuclear transcription factor Y subunit A-8 OS=Arab...	[more]
NFYA9_ARATH	2.442e-14	75.00	Nuclear transcription factor Y subunit A-9 OS=Arab...	[more]
NFYA5_ARATH	7.107e-14	68.63	Nuclear transcription factor Y subunit A-5 OS=Arab...	[more]
NFYA6_ARATH	6.016e-13	66.67	Nuclear transcription factor Y subunit A-6 OS=Arab...	[more]
NFYA1_ARATH	3.899e-12	66.67	Nuclear transcription factor Y subunit A-1 OS=Arab...	[more]

BLAST of GH720304 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT3G05690.1	1.989e-23	44.83	\| Symbols: UNE8, ATHAP2B, HAP2B, NF-YA2 \| nuclear ...	[more]
AT5G06510.2	3.392e-23	44.10	\| Symbols: NF-YA10 \| nuclear factor Y, subunit A10...	[more]
AT5G06510.3	3.392e-23	44.10	\| Symbols: NF-YA10 \| nuclear factor Y, subunit A10...	[more]
AT5G06510.1	3.392e-23	44.10	\| Symbols: NF-YA10 \| nuclear factor Y, subunit A10...	[more]
AT1G30500.2	9.560e-18	76.00	\| Symbols: NF-YA7 \| nuclear factor Y, subunit A7 \|...	[more]
AT1G30500.1	9.560e-18	76.00	\| Symbols: NF-YA7 \| nuclear factor Y, subunit A7 \|...	[more]
AT2G34720.1	1.249e-17	76.00	\| Symbols: NF-YA4 \| nuclear factor Y, subunit A4 \|...	[more]
AT1G72830.3	8.947e-16	68.63	\| Symbols: HAP2C, ATHAP2C, NF-YA3 \| nuclear factor...	[more]
AT1G72830.1	8.947e-16	68.63	\| Symbols: HAP2C, ATHAP2C, NF-YA3 \| nuclear factor...	[more]
AT1G17590.4	1.169e-15	68.63	\| Symbols: NF-YA8 \| nuclear factor Y, subunit A8 \|...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001289	CCAAT-binding transcription factor, subunit B	PANTHER	PTHR12632	TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED	coord: 7..164 score: 2.3
IPR001289	CCAAT-binding transcription factor, subunit B	PFAM	PF02045	CBFB_NFYA	coord: 7..53 score: 2.2
IPR001289	CCAAT-binding transcription factor, subunit B	SMART	SM00521	no description	coord: 1..56 score: 1.8
IPR001289	CCAAT-binding transcription factor, subunit B	PROFILE	PS51152	NFYA_HAP2_2	coord: 1..56 score: 28
None	No IPR available	PANTHER	PTHR12632:SF3	TRANSCRIPTION FACTOR	coord: 7..164 score: 2.3
None	No IPR available	SEG	seg	seg	coord: 57..72 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>GH720304 ID=GH720304; Name=GH720304; organism=Pisum sativum; type=EST; length=565bp
TTCGAGCGGCCGCCCNGGGCAGGTACAAAATTTTATACCAAACCCATTTG
AATTCTTTACGAAATCAAGCTTTAAGATTACAGCAGTTGCCAGCTGCTGT
AACCCATTTGGTGGGCATGATGACGCCACGCCCACCATCGATCATGTCTG
CCAACGAGTGGACAGTAGGTCCAAGATGATTGACGGAAAAGCTGCCAAGA
TTTCCCCTCGAATACGAACTAGTCACCTCGAACCCAGAAATATTCGGACT
GCTACCGTTAGTTTCTTTTGACGAGTTTAAAGTTCCAACATCAGATTCAA
AGAGTTCAGAACTCGGAGAGCCGCTGCACTGCAATTGTTGACCGCAGAAT
TTATGCACTTTGCTTACACTTTTACCATCTCCTTTGGATGATTTCTTCGT
GTTCAAGAAACGGCCACCGCACCCTCTTGGTCGCCGCATTGCATGGAGAT
GACGAGACTCGTGCATGTAGGGCTTGCGGCGTTTGATCAATTTCTGACCA
AGAACAGCTTTGGCGCGGGACTGTCGGCGTCTAATGATTCCATGGTACCT
CGGCCGCGACCACGC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003700	DNA-binding transcription factor activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **INTERPRO**
Term	Definition
IPR001289	NFYA

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