BU964318
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EST Overview
Libraries
Unigenes
This EST is part of the following unigenes:
Analyses
This EST is derived from or has results from the following analyses
Homology
BLAST of BU964318 vs. TrEMBL
Match: Q6YA92_PEA (Cytochrome P450 OS=Pisum sativum PE=2 SV=1) HSP 1 Score: 234.958 bits (598), Expect = 9.902e-71 Identity = 117/128 (91.41%), Postives = 117/128 (91.41%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
MSPLFYYSFLSLTFIITIKILLQTQSRRLKNL NLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR
Sbjct: 1 MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 128 HSP 2 Score: 58.5362 bits (140), Expect = 9.902e-71 Identity = 29/29 (100.00%), Postives = 29/29 (100.00%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQ 494
RITTIDVLSNNRLTSFLGVRTDEANRLIQ
Sbjct: 129 RITTIDVLSNNRLTSFLGVRTDEANRLIQ 157 HSP 3 Score: 170.244 bits (430), Expect = 3.409e-68 Identity = 84/100 (84.00%), Postives = 84/100 (84.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PEVLNKAK IETQVGKNKIIDEQDLPKLPYLQNIISE YSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG
Sbjct: 324 PEVLNKAKEEIETQVGKNKIIDEQDLPKLPYLQNIISEALKLHPPAPLLLPHYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 423 HSP 4 Score: 114.775 bits (286), Expect = 3.409e-68 Identity = 55/72 (76.39%), Postives = 58/72 (80.56%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
++ G+ KLMAFGIGRRACPGLSLAQRTVGLNCG CWFQCFEW KRE L KVLPCLKRSPFKALC
Sbjct: 420 EKEGEVNKLMAFGIGRRACPGLSLAQRTVGLNCGACWFQCFEW-KRESEEKLDMMEGKVLPCLKRSPFKALC 490
BLAST of BU964318 vs. TrEMBL
Match: Q6WNQ9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula PE=2 SV=1) HSP 1 Score: 201.06 bits (510), Expect = 5.671e-59 Identity = 97/125 (77.60%), Postives = 103/125 (82.40%), Query Frame = 1
Query: 34 LFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
LFYYS LSL+FIITIKILL+ SRRLKNL NLHHLKHPLHRTF TLSQ YGDI SLWFGSRLVVV+SS SL ECF KND++LANRPRFLTGKYIFY+YTTLGS SYGDHWRNLR
Sbjct: 2 LFYYSLLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLR 126 HSP 2 Score: 53.1434 bits (126), Expect = 5.671e-59 Identity = 27/31 (87.10%), Postives = 27/31 (87.10%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RITTIDVLSNNRL SFLGVR DE NRLIQ L
Sbjct: 127 RITTIDVLSNNRLNSFLGVRRDETNRLIQKL 157 HSP 3 Score: 149.058 bits (375), Expect = 2.012e-46 Identity = 71/99 (71.72%), Postives = 77/99 (77.78%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKE 807
PEVL KAK ++TQ+GKNK++DEQDL KLPYLQNIISE YSSEDCTIG FNVPKDTIILTNVW IHRDP W+DALSFKPERFEKE
Sbjct: 325 PEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKE 423 HSP 4 Score: 63.1586 bits (152), Expect = 2.012e-46 Identity = 34/65 (52.31%), Postives = 41/65 (63.08%), Query Frame = 3
Query: 822 KLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
K+MAFG+GRRACPGLSLAQRTVG G QCFEW +RE L K + + P +A+C
Sbjct: 428 KVMAFGLGRRACPGLSLAQRTVGFTVGLL-IQCFEW-ERESEEKLDMMEGKGITMPMKIPLRAMC 490
BLAST of BU964318 vs. TrEMBL
Match: Q2ENF7_ASTMO (Isoflavone 3'-hydroxylase (Fragment) OS=Astragalus mongholicus PE=2 SV=1) HSP 1 Score: 164.081 bits (414), Expect = 4.870e-45 Identity = 73/85 (85.88%), Postives = 80/85 (94.12%), Query Frame = 1
Query: 154 NLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
NLHHLKHPLHRTF TLSQKYGDI+SLWFGSRLVVV++S S+ +ECF KNDVVLANRP+FLTGKYIFY+YTTLGS SYGDHWRNLR
Sbjct: 2 NLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLR 86 HSP 2 Score: 43.5134 bits (101), Expect = 4.870e-45 Identity = 22/31 (70.97%), Postives = 25/31 (80.65%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RITTIDVLSN+RL SF GVR DE RL++ L
Sbjct: 87 RITTIDVLSNHRLNSFHGVRKDETLRLVEKL 117
BLAST of BU964318 vs. TrEMBL
Match: Q9ZRW6_CICAR (Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2 SV=1) HSP 1 Score: 162.925 bits (411), Expect = 1.159e-43 Identity = 80/125 (64.00%), Postives = 92/125 (73.60%), Query Frame = 1
Query: 34 LFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
L YS L+F I++L Q SR+ KNL NLHHLK PLHRTF LS+ YGDI+SLWFGSRLVVV+SS S ++CF KNDVVLANRPRFL+GKYIFY+YTTLGS SY +HWRNLR
Sbjct: 6 LLCYSLFYLSFFFIIRLLFQ--SRKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSKTYGDIISLWFGSRLVVVVSSLSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYSEHWRNLR 128 HSP 2 Score: 40.0466 bits (92), Expect = 1.159e-43 Identity = 18/28 (64.29%), Postives = 23/28 (82.14%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLI 491
RIT++DVLSN+R+ +F GVR DE RLI
Sbjct: 129 RITSLDVLSNHRINNFSGVRRDETQRLI 156 HSP 3 Score: 117.472 bits (293), Expect = 9.607e-32 Identity = 52/103 (50.49%), Postives = 70/103 (67.96%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PE+L K K ++ VG+++++DE DLPKL YL+N+I E +S+DC IGG+ VP+DTI+L N WAIHRDP WS+A SFKPERF+K+G E
Sbjct: 322 PEILKKVKDELDIHVGQDRLVDESDLPKLTYLKNVIYETLRLCTPAPLLLPHSTSDDCIIGGYKVPRDTIVLINAWAIHRDPKSWSEATSFKPERFDKKGEIE 424 HSP 4 Score: 45.0542 bits (105), Expect = 9.607e-32 Identity = 19/44 (43.18%), Postives = 30/44 (68.18%), Query Frame = 3
Query: 804 RRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++G+ K++AFG+GRR CPG +LA RT+ + QCF+W +
Sbjct: 419 KKGEIEKVIAFGMGRRVCPGEALALRTISMTLALL-VQCFDWKR 461 HSP 5 Score: 20.7866 bits (42), Expect = 9.607e-32 Identity = 8/24 (33.33%), Postives = 13/24 (54.17%), Query Frame = 2
Query: 932 KARXEEKLDMMEGKGIAMPXKIPI 1003
K ++K+DM E G + IP+
Sbjct: 460 KRTSDDKIDMAERDGFVLTKLIPL 483
BLAST of BU964318 vs. TrEMBL
Match: Q9MBE4_LOTJA (Cytochrome P450 OS=Lotus japonicus PE=2 SV=1) HSP 1 Score: 162.925 bits (411), Expect = 3.342e-43 Identity = 79/123 (64.23%), Postives = 91/123 (73.98%), Query Frame = 1
Query: 40 YYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
YYS + IK+ L SR+ KNL NLHHLK PLHRTF LS+KYGD+ SLWFG+RLVVV+SS + V+ECF KNDVVLANRPRFL+GKYIFY+YTTLGS SYG+HWRNLR
Sbjct: 8 YYSLFYVALFAIIKLFLG--SRKFKNLPPGPTSLPIIGNLHHLKRPLHRTFRALSEKYGDVFSLWFGNRLVVVVSSFADVQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 128 HSP 2 Score: 38.5058 bits (88), Expect = 3.342e-43 Identity = 18/31 (58.06%), Postives = 24/31 (77.42%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RIT++DVLSN+R+ SF +R DE RLI+ L
Sbjct: 129 RITSLDVLSNHRINSFSPIRRDETTRLIRKL 159 HSP 3 Score: 119.013 bits (297), Expect = 4.636e-32 Identity = 51/103 (49.51%), Postives = 73/103 (70.87%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PEVL K K ++T VG+++++DE D+PKL YL+N+I+E +S+DCTIGG+ VP+DTI+L N WA+HRDP W++A +FKPERF+K+G E
Sbjct: 323 PEVLEKIKAELDTHVGQDRLVDESDIPKLTYLKNVINETLRLYTPAPLLLPHSASDDCTIGGYKVPRDTIVLINAWALHRDPQLWTEATTFKPERFDKKGELE 425 HSP 4 Score: 45.0542 bits (105), Expect = 4.636e-32 Identity = 26/71 (36.62%), Postives = 37/71 (52.11%), Query Frame = 3
Query: 804 RRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
++G+ KL+ FG+GRRACPG LA R + + QCF+W KR + +K P KA+C
Sbjct: 420 KKGELEKLIPFGLGRRACPGELLAIRAISMTLALL-IQCFDW-KRVSDEEIDMGERDGFVLMKSIPVKAMC 488
BLAST of BU964318 vs. TrEMBL
Match: Q9ZWF2_GLYEC (Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-31 PE=2 SV=1) HSP 1 Score: 160.614 bits (405), Expect = 4.356e-43 Identity = 78/120 (65.00%), Postives = 92/120 (76.67%), Query Frame = 1
Query: 49 FLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
+L+L FI I I ++R+ KNL NLHHLK PLHRTF LS+KYG ++SLWFGSRLVVV+SSAS ++CF KNDVVLANRPRFL+GKYIFY+YTTLGS SYG+HWRNLR
Sbjct: 13 YLALFFIFNIVI----RARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVISLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 128 HSP 2 Score: 40.4318 bits (93), Expect = 4.356e-43 Identity = 18/28 (64.29%), Postives = 22/28 (78.57%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLI 491
RIT +DVLSN+R+ SF G+R DE RLI
Sbjct: 129 RITALDVLSNHRINSFSGIRRDETQRLI 156 HSP 3 Score: 118.242 bits (295), Expect = 6.038e-32 Identity = 52/103 (50.49%), Postives = 72/103 (69.90%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PEVL K K ++T VG+++++DE DLPKL YL+N+I+E +S++C IGG+ VP+DTI+L N WAIHRDP W++A +FKPERFEK+G E
Sbjct: 322 PEVLKKVKDELDTHVGQDRLVDESDLPKLSYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGELE 424 HSP 4 Score: 45.4394 bits (106), Expect = 6.038e-32 Identity = 20/43 (46.51%), Postives = 29/43 (67.44%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
+++G+ KL+AFG+GRRACPG LA R + + QCF+W
Sbjct: 418 EKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALL-IQCFDW 459
BLAST of BU964318 vs. TrEMBL
Match: Q6WNR0_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula PE=2 SV=1) HSP 1 Score: 160.614 bits (405), Expect = 2.779e-42 Identity = 77/122 (63.11%), Postives = 92/122 (75.41%), Query Frame = 1
Query: 43 YSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
YS L+ I++L Q SR+ KNL NLHHLK PL+RTF L++KYG+++SLWFGSRLVVV+SS S +ECF KNDVVLANRPRFL+GKYIFY+YTTLGS SYG+HWRNLR
Sbjct: 9 YSLFYLSIFFIIRLLFQ--SRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 128 HSP 2 Score: 37.7354 bits (86), Expect = 2.779e-42 Identity = 17/31 (54.84%), Postives = 24/31 (77.42%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RIT++DVLSN+R+ +F +R DE RLI+ L
Sbjct: 129 RITSLDVLSNHRINNFAPIRRDETQRLIKKL 159 HSP 3 Score: 114.39 bits (285), Expect = 1.103e-30 Identity = 49/103 (47.57%), Postives = 71/103 (68.93%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PEVL K + ++T VG+++++DE DLPKL YL+N+I E ++++C +GG+ VP+DTI+L N WAIHRDP WS+A +FKPERF+K+G E
Sbjct: 322 PEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDKKGELE 424 HSP 4 Score: 45.0542 bits (105), Expect = 1.103e-30 Identity = 26/71 (36.62%), Postives = 37/71 (52.11%), Query Frame = 3
Query: 804 RRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
++G+ K++AFG+GRRACPG LA R + + QCF+W KR + K P KA+C
Sbjct: 419 KKGELEKMIAFGMGRRACPGEGLALRAISMTLALL-VQCFDW-KRINDEKIDMSERDGFTMTKLLPLKAMC 487
BLAST of BU964318 vs. TrEMBL
Match: Q9XFX0_CICAR (Cytochrome P450 monooxygenase OS=Cicer arietinum GN=cyp81E3v2 PE=2 SV=1) HSP 1 Score: 158.303 bits (399), Expect = 2.779e-42 Identity = 77/124 (62.10%), Postives = 89/124 (71.77%), Query Frame = 1
Query: 37 FYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
F YS L+ ++L Q SR+ KNL N HHLK PLHRTF LS+ YGDI+SLWFGSRLVVV+SS S ++CF KNDVVLANRPRFL+GKYIFY+YTTLGS SY +HWRNLR
Sbjct: 7 FAYSLFYLSIFFIFRLLFQ--SRKFKNLPPGPPSLPIIGNFHHLKRPLHRTFKGLSKTYGDIISLWFGSRLVVVVSSLSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYSEHWRNLR 128 HSP 2 Score: 40.0466 bits (92), Expect = 2.779e-42 Identity = 18/28 (64.29%), Postives = 23/28 (82.14%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLI 491
RIT++DVLSN+R+ +F GVR DE RLI
Sbjct: 129 RITSLDVLSNHRINNFSGVRRDETQRLI 156 HSP 3 Score: 117.857 bits (294), Expect = 7.864e-32 Identity = 52/103 (50.49%), Postives = 71/103 (68.93%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PEVL K + ++T VG+++++DE DLPKL YL+N+I E +S+DC IGG+ VP+DTI+L N WAIHRDP WS+A +FKPERF+K+G E
Sbjct: 322 PEVLKKVRDELDTHVGQDRLVDESDLPKLTYLKNVIYETLRLCTPAPLLLPHSTSDDCIIGGYKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIE 424 HSP 4 Score: 45.4394 bits (106), Expect = 7.864e-32 Identity = 19/44 (43.18%), Postives = 30/44 (68.18%), Query Frame = 3
Query: 804 RRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++G+ K++AFG+GRR CPG +LA RT+ + QCF+W +
Sbjct: 419 KKGEIEKVIAFGMGRRVCPGEALALRTISMTLALL-IQCFDWKR 461
BLAST of BU964318 vs. TrEMBL
Match: Q9SML1_CICAR (Cytochrome P450 monooxygenase (Fragment) OS=Cicer arietinum GN=cyp81E4 PE=2 SV=1) HSP 1 Score: 132.109 bits (331), Expect = 6.755e-41 Identity = 64/100 (64.00%), Postives = 74/100 (74.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
P+VL ++K TQ+GK+K+I+E DL KLPYLQNII+E YSSED T+GGFNVPKDTIILTNVWAIHRDP WSDA SFKPERFE +G
Sbjct: 31 PDVLKQSKRRNRTQIGKDKLIEEHDLSKLPYLQNIINETLRLHPPAPLSLPHYSSEDFTLGGFNVPKDTIILTNVWAIHRDPKYWSDASSFKPERFEIDG 130 HSP 2 Score: 61.6178 bits (148), Expect = 6.755e-41 Identity = 36/69 (52.17%), Postives = 41/69 (59.42%), Query Frame = 3
Query: 810 GK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
G+ KL+AFG+GRRACPG SLA RTVGL G QCFEW KRE + K L P KA+C
Sbjct: 130 GELNKLIAFGLGRRACPGQSLAHRTVGLTLGLL-IQCFEW-KRETEEKIDLREGKGLTMPMGVPLKAMC 196
BLAST of BU964318 vs. TrEMBL
Match: Q6WNQ8_MEDTR (CYP81E8 OS=Medicago truncatula PE=2 SV=1) HSP 1 Score: 149.828 bits (377), Expect = 1.016e-38 Identity = 76/126 (60.32%), Postives = 87/126 (69.05%), Query Frame = 1
Query: 37 FYYSFLSLTF--IITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
FY S + F IIT+K+ T SR+ KNL NLH LK PLH TF TLSQKYG I SLWFGSRLVVV+SS ++ +ECF KND+VLANRP FLTGKYI Y+ TT+ YGDHWRNLR
Sbjct: 4 FYLSLIISLFFLIITLKVFFNT-SRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLR 128 HSP 2 Score: 36.5798 bits (83), Expect = 1.016e-38 Identity = 18/31 (58.06%), Postives = 24/31 (77.42%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RI +I++LS++RL SFL +R DE RLIQ L
Sbjct: 129 RILSIEILSSHRLNSFLEIRRDEIMRLIQKL 159 HSP 3 Score: 112.079 bits (279), Expect = 2.535e-33 Identity = 48/100 (48.00%), Postives = 67/100 (67.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PE++ KAK ++T +G ++ +DE D+ KLPYLQ+I+ E SSED ++GG+N+P++TI++ N W IHRDP WSD FKPERFEKEG
Sbjct: 322 PEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKEG 421 HSP 4 Score: 56.225 bits (134), Expect = 2.535e-33 Identity = 30/72 (41.67%), Postives = 43/72 (59.72%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
++ G+ KL++FG+GRRACPG +L+QRT GL G QCFEW KR + K + K++ A+C
Sbjct: 418 EKEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLL-IQCFEW-KRIGEEKIDMVEAKGITAGKKTSLNAMC 487
BLAST of BU964318 vs. SwissProt
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2) HSP 1 Score: 159.073 bits (401), Expect = 7.778e-44 Identity = 78/120 (65.00%), Postives = 91/120 (75.83%), Query Frame = 1
Query: 49 FLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
+L+L FI I I ++R+ KNL NLHHLK PLHRTF LS+KYG + SLWFGSRLVVV+SSAS ++CF KNDVVLANRPRFL+GKYIFY+YTTLGS SYG+HWRNLR
Sbjct: 13 YLALFFIFNIVI----RARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 128 HSP 2 Score: 40.4318 bits (93), Expect = 7.778e-44 Identity = 18/28 (64.29%), Postives = 22/28 (78.57%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLI 491
RIT +DVLSN+R+ SF G+R DE RLI
Sbjct: 129 RITALDVLSNHRINSFSGIRRDETQRLI 156 HSP 3 Score: 118.242 bits (295), Expect = 3.738e-33 Identity = 52/103 (50.49%), Postives = 72/103 (69.90%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PEVL K K ++T VG+++++DE DLPKL YL+N+I+E +S++C IGG+ VP+DTI+L N WAIHRDP W++A +FKPERFEK+G E
Sbjct: 322 PEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGELE 424 HSP 4 Score: 45.4394 bits (106), Expect = 3.738e-33 Identity = 20/43 (46.51%), Postives = 29/43 (67.44%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
+++G+ KL+AFG+GRRACPG LA R + + QCF+W
Sbjct: 418 EKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALL-IQCFDW 459
BLAST of BU964318 vs. SwissProt
Match: C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1) HSP 1 Score: 105.145 bits (261), Expect = 8.115e-32 Identity = 47/99 (47.47%), Postives = 66/99 (66.67%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKE 807
P+V++KA+ I+ +VG +++I+E DL +LPYL+NI+ E +SEDC IG +++P+ T +L N WAIHRDP W D SFKPERFEKE
Sbjct: 326 PDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKE 424 HSP 2 Score: 53.1434 bits (126), Expect = 8.115e-32 Identity = 25/38 (65.79%), Postives = 28/38 (73.68%), Query Frame = 3
Query: 822 KLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
KL+AFG+GRRACPG LAQR VGL G QCFEW +
Sbjct: 429 KLLAFGLGRRACPGSGLAQRIVGLALGSL-IQCFEWER 465 HSP 3 Score: 21.1718 bits (43), Expect = 8.115e-32 Identity = 8/18 (44.44%), Postives = 12/18 (66.67%), Query Frame = 2
Query: 950 KLDMMEGKGIAMPXKIPI 1003
++DM EG G +P IP+
Sbjct: 470 EVDMKEGVGNTVPKAIPL 487 HSP 4 Score: 89.7373 bits (221), Expect = 1.370e-21 Identity = 54/128 (42.19%), Postives = 73/128 (57.03%), Query Frame = 1
Query: 43 YSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKY-----GDIVSLWFGSRLVVVISSASLV-EECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
YS SL F+I L+ + + L +L LK P+HRT + S+ G ++SL GSRLV V+SS + EECF KNDVVLANRP+ + GK++ Y+ T + + YGDHWRNLR
Sbjct: 11 YSIFSLIFLIISFKFLKPKKQNLP--PSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHWRNLR 136 HSP 5 Score: 35.039 bits (79), Expect = 1.370e-21 Identity = 17/31 (54.84%), Postives = 21/31 (67.74%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
R+ TI++ S +RL FL VRTDE RLI L
Sbjct: 137 RLCTIEIFSTHRLNCFLYVRTDEVRRLISRL 167
BLAST of BU964318 vs. SwissProt
Match: C81F1_ARATH (Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CYP81F1 PE=2 SV=2) HSP 1 Score: 100.908 bits (250), Expect = 1.187e-27 Identity = 44/103 (42.72%), Postives = 65/103 (63.11%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PEVL KA+ I+ ++GK+++IDE D+ LPYLQN++SE ++D IGG++VP+DTI++ N WAIHRDP W + F P+R+ GS+
Sbjct: 320 PEVLEKARSEIDEKIGKDRLIDESDIAVLPYLQNVVSETFRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFNPDRYNDGCGSD 422 HSP 2 Score: 44.2838 bits (103), Expect = 1.187e-27 Identity = 22/36 (61.11%), Postives = 22/36 (61.11%), Query Frame = 3
Query: 822 KLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
KLM FG GRR CPG L QR V L G QCFEW
Sbjct: 427 KLMPFGNGRRTCPGAGLGQRIVTLALGSL-IQCFEW 461 HSP 3 Score: 105.531 bits (262), Expect = 5.112e-22 Identity = 56/126 (44.44%), Postives = 79/126 (62.70%), Query Frame = 1
Query: 34 LFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFI-KNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
+ Y+ L L F++ L ++++R NL +LH +K P+HR S +YG I SL FGSR VVVI+S SL +E F +ND+VL++RP LT KY+ Y++TT+G+ YGDHWRNLR
Sbjct: 1 MLYFILLPLLFLVISYKFLYSKTQRF-NLPPGPPSRPFVGHLHLMKPPIHRLLQRYSNQYGPIFSLRFGSRRVVVITSPSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHTTVGTAPYGDHWRNLR 125
BLAST of BU964318 vs. SwissProt
Match: F3PH_ARATH (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana GN=CYP75B1 PE=1 SV=1) HSP 1 Score: 93.9745 bits (232), Expect = 8.091e-22 Identity = 43/104 (41.35%), Postives = 66/104 (63.46%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERF----EKEG 810
P+++ KA+ ++ VG+++ ++E D+ +LPYLQ +I E +SE C I G+++PK + +LTN+WAI RDP +WSD L+FKPERF EK G
Sbjct: 322 PDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPGGEKSG 425 HSP 2 Score: 31.5722 bits (70), Expect = 8.091e-22 Identity = 14/27 (51.85%), Postives = 18/27 (66.67%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTV 887
+G +L+ FG GRR C GLSL RT+
Sbjct: 429 KGSDFELIPFGAGRRICAGLSLGLRTI 455 HSP 3 Score: 55.8398 bits (133), Expect = 4.645e-7 Identity = 39/129 (30.23%), Postives = 58/129 (44.96%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIKILLQTQSRRLKN-LXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
M+ LF L+ + ++I ++R N L NL H+ HRT + + YG I+ L G VVV +S S+ E+ +D A+RP K++ Y+Y L YG WR LR
Sbjct: 1 MATLFLTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMVTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRWRLLR 129
BLAST of BU964318 vs. SwissProt
Match: C93A3_SOYBN (Cytochrome P450 93A3 OS=Glycine max GN=CYP93A3 PE=2 SV=1) HSP 1 Score: 82.4185 bits (202), Expect = 1.053e-21 Identity = 40/103 (38.83%), Postives = 58/103 (56.31%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
P VL KA+ ++ VGK++I++E D+ LPYLQ I+ E SS + G+++P T + NVWAI RDP W + L F+PERF + G S+
Sbjct: 327 PGVLEKARQEMDAVVGKSRIVEESDIANLPYLQGIVRETLRLHPAGPLLFRE-SSRRAVVCGYDIPAKTRLFVNVWAIGRDPNHWENPLEFRPERFVENGKSQ 428 HSP 2 Score: 42.743 bits (99), Expect = 1.053e-21 Identity = 21/41 (51.22%), Postives = 26/41 (63.41%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
RG+ L+ FG GRRACPG SLA + V +N QCF+W
Sbjct: 432 RGQHYHLLPFGSGRRACPGTSLALQVVHVNLAVL-IQCFQW 471
BLAST of BU964318 vs. SwissProt
Match: C93A1_SOYBN (Cytochrome P450 93A1 OS=Glycine max GN=CYP93A1 PE=2 SV=1) HSP 1 Score: 81.6481 bits (200), Expect = 1.898e-20 Identity = 38/103 (36.89%), Postives = 59/103 (57.28%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
P+VL KA+ I+ VGK+++++E D+ LPYLQ I+ E SS+ + G+++P T + NVWAI RDP W F+PERF ++G ++
Sbjct: 326 PDVLEKARQEIDAVVGKSRMVEESDIANLPYLQAIVRETLRLHPGGPLVVRE-SSKSAVVCGYDIPAKTRLFVNVWAIGRDPNHWEKPFEFRPERFIRDGQNQ 427 HSP 2 Score: 39.2762 bits (90), Expect = 1.898e-20 Identity = 19/41 (46.34%), Postives = 24/41 (58.54%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
RG+ + FG GRR CPG SLA + V +N QCF+W
Sbjct: 431 RGQHYHFIPFGSGRRTCPGASLAWQVVPVNLAII-IQCFQW 470
BLAST of BU964318 vs. SwissProt
Match: C82A1_PEA (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum GN=CYP82A1 PE=2 SV=2) HSP 1 Score: 90.8929 bits (224), Expect = 5.423e-20 Identity = 42/96 (43.75%), Postives = 56/96 (58.33%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERF 798
P VL K K + T +GK + ++E D+ KL YL II E +EDCTIGG+++ K T ++ N+W IHRDP+ W D L FKPERF
Sbjct: 360 PHVLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIGGYHIKKGTRLMPNLWKIHRDPSVWPDPLEFKPERF 455 HSP 2 Score: 28.4906 bits (62), Expect = 5.423e-20 Identity = 13/27 (48.15%), Postives = 17/27 (62.96%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTV 887
RG+ +L+ FG GRR C G+SL V
Sbjct: 465 RGQNFELLPFGSGRRMCAGMSLGLHMV 491 HSP 3 Score: 63.929 bits (154), Expect = 1.706e-9 Identity = 28/76 (36.84%), Postives = 47/76 (61.84%), Query Frame = 1
Query: 181 HRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
H+T L KYG I ++ G+ +V+S+ L +ECF KND+V+++RP+ + + + Y+ +G YG +WR LR
Sbjct: 58 HKTLGALVDKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGWAPYGAYWRQLR 133
BLAST of BU964318 vs. SwissProt
Match: C76C1_ARATH (Cytochrome P450 76C1 OS=Arabidopsis thaliana GN=CYP76C1 PE=2 SV=1) HSP 1 Score: 80.8777 bits (198), Expect = 1.194e-19 Identity = 39/96 (40.62%), Postives = 55/96 (57.29%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERF 798
P+ + KA+ I+ +G+N I++E D+ KLPYLQ ++ E + D I GF V KDT +L NVWAI RDP+ W + F+PERF
Sbjct: 331 PKTMAKAQAEIDCVIGQNGIVEESDISKLPYLQAVVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSVWDNPSQFEPERF 426 HSP 2 Score: 37.3502 bits (85), Expect = 1.194e-19 Identity = 18/41 (43.90%), Postives = 24/41 (58.54%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
RG+ +L FG GRR CPG+ LA +TV L + F+W
Sbjct: 434 RGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYS-FDW 473
BLAST of BU964318 vs. SwissProt
Match: C93A2_SOYBN (Cytochrome P450 93A2 OS=Glycine max GN=CYP93A2 PE=2 SV=1) HSP 1 Score: 78.5666 bits (192), Expect = 1.554e-19 Identity = 40/103 (38.83%), Postives = 56/103 (54.37%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
P VL KA+ I+ VG ++II+E D+ LPYLQ I+ E SS+ + G+ +P T + NVWAI RDP W + F+PERF + G S+
Sbjct: 319 PCVLEKARQEIDAVVGNSRIIEESDIVNLPYLQAIVRETLRIHPGGPLIVRE-SSKSVVVCGYEIPAKTRLFVNVWAIGRDPNHWENPFEFRPERFFENGQSQ 420 HSP 2 Score: 39.2762 bits (90), Expect = 1.554e-19 Identity = 19/41 (46.34%), Postives = 25/41 (60.98%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
RG+ + FG GRR+CPG SLA + V +N QCF+W
Sbjct: 424 RGQHYHFIPFGSGRRSCPGTSLALQIVHVNLA-IMIQCFQW 463
BLAST of BU964318 vs. SwissProt
Match: C82A3_SOYBN (Cytochrome P450 82A3 OS=Glycine max GN=CYP82A3 PE=2 SV=1) HSP 1 Score: 89.3521 bits (220), Expect = 2.018e-19 Identity = 42/96 (43.75%), Postives = 58/96 (60.42%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERF 798
P L KAK I+ Q+GK++ I E D+ KL YLQ I+ E +E+C +GG+++ K T ++ N+W IHRDP+ WSD L FKPERF
Sbjct: 343 PLALGKAKEEIDMQIGKDEYIRESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHRDPSVWSDPLEFKPERF 438 HSP 2 Score: 28.1054 bits (61), Expect = 2.018e-19 Identity = 13/27 (48.15%), Postives = 16/27 (59.26%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTV 887
RG +L+ FG GRR C G+SL V
Sbjct: 448 RGHNFELLPFGSGRRVCAGMSLGLNMV 474
BLAST of BU964318 vs. TAIR peptide
Match: AT4G37360.1 (| Symbols: CYP81D2 | cytochrome P450, family 81, subfamily D, polypeptide 2 | chr4:17567124-17568858 REVERSE LENGTH=499) HSP 1 Score: 119.013 bits (297), Expect = 3.542e-38 Identity = 51/100 (51.00%), Postives = 70/100 (70.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PEVLNKA+ I+ +G +++++E D+P LPYLQNI+SE +S+DC +GG+++P+ T++LTN WAIHRDP W D SFKPERFEKEG
Sbjct: 320 PEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKEG 419 HSP 2 Score: 52.373 bits (124), Expect = 3.542e-38 Identity = 25/45 (55.56%), Postives = 31/45 (68.89%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++ G+ KLM FG+GRRACPG LAQR V L+ G QCFEW +
Sbjct: 416 EKEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSL-IQCFEWER 459 HSP 3 Score: 26.1794 bits (56), Expect = 3.542e-38 Identity = 10/20 (50.00%), Postives = 14/20 (70.00%), Query Frame = 2
Query: 944 EEKLDMMEGKGIAMPXKIPI 1003
EE++DM EG G+ MP P+
Sbjct: 462 EEEVDMTEGPGLTMPKARPL 481 HSP 4 Score: 94.3597 bits (233), Expect = 1.135e-19 Identity = 54/127 (42.52%), Postives = 75/127 (59.06%), Query Frame = 1
Query: 34 LFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGD--IVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
+F + F+ L+ I I + R+L +L LK PLHR F ++SQ GD I+SL G+RL+ V+SS S+ EECF KNDV+LANR ++ K+I Y +T+ S SY +HWRNLR
Sbjct: 7 IFTFCFIVLSLIFLIGRI----KRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRNLR 129
BLAST of BU964318 vs. TAIR peptide
Match: AT1G66540.1 (| Symbols: | Cytochrome P450 superfamily protein | chr1:24824837-24826502 FORWARD LENGTH=386) HSP 1 Score: 118.627 bits (296), Expect = 3.564e-38 Identity = 63/148 (42.57%), Postives = 86/148 (58.11%), Query Frame = 1
Query: 388 DHWRNLRV*QPLMFSLTTV----LPLFWEFGQTKQIDLYKYYKAC---PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
DH +L+V QP ++ T+ L L T + L + PEVL K + I+ Q+G +++++E D+P LPYLQNI+SE SSEDC +GG+++P T++L NVWAIHRDP W D SFKPERFEKEG
Sbjct: 159 DHLLSLQVSQPEYYTDHTIKGTMLSLILAGTDTSAVTLEWALSSLLNNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVSETLRLYPAGPLLVPHISSEDCKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPERFEKEG 306 HSP 2 Score: 50.447 bits (119), Expect = 3.564e-38 Identity = 23/45 (51.11%), Postives = 31/45 (68.89%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++ G+ KL+ FG+GRRACPG LA+R V L+ G QCFEW +
Sbjct: 303 EKEGETHKLLTFGLGRRACPGSGLARRLVSLSLGSL-IQCFEWER 346 HSP 3 Score: 28.4906 bits (62), Expect = 3.564e-38 Identity = 11/20 (55.00%), Postives = 15/20 (75.00%), Query Frame = 2
Query: 944 EEKLDMMEGKGIAMPXKIPI 1003
EE++DM EG G+ MP IP+
Sbjct: 349 EEEVDMTEGGGLTMPRAIPL 368
BLAST of BU964318 vs. TAIR peptide
Match: AT1G66540.2 (| Symbols: | Cytochrome P450 superfamily protein | chr1:24825446-24826502 FORWARD LENGTH=327) HSP 1 Score: 118.627 bits (296), Expect = 3.579e-38 Identity = 63/148 (42.57%), Postives = 86/148 (58.11%), Query Frame = 1
Query: 388 DHWRNLRV*QPLMFSLTTV----LPLFWEFGQTKQIDLYKYYKAC---PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
DH +L+V QP ++ T+ L L T + L + PEVL K + I+ Q+G +++++E D+P LPYLQNI+SE SSEDC +GG+++P T++L NVWAIHRDP W D SFKPERFEKEG
Sbjct: 100 DHLLSLQVSQPEYYTDHTIKGTMLSLILAGTDTSAVTLEWALSSLLNNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVSETLRLYPAGPLLVPHISSEDCKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPERFEKEG 247 HSP 2 Score: 50.447 bits (119), Expect = 3.579e-38 Identity = 23/45 (51.11%), Postives = 31/45 (68.89%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++ G+ KL+ FG+GRRACPG LA+R V L+ G QCFEW +
Sbjct: 244 EKEGETHKLLTFGLGRRACPGSGLARRLVSLSLGSL-IQCFEWER 287 HSP 3 Score: 28.4906 bits (62), Expect = 3.579e-38 Identity = 11/20 (55.00%), Postives = 15/20 (75.00%), Query Frame = 2
Query: 944 EEKLDMMEGKGIAMPXKIPI 1003
EE++DM EG G+ MP IP+
Sbjct: 290 EEEVDMTEGGGLTMPRAIPL 309
BLAST of BU964318 vs. TAIR peptide
Match: AT4G37370.1 (| Symbols: CYP81D8 | cytochrome P450, family 81, subfamily D, polypeptide 8 | chr4:17569971-17571678 REVERSE LENGTH=497) HSP 1 Score: 119.783 bits (299), Expect = 1.823e-36 Identity = 52/103 (50.49%), Postives = 73/103 (70.87%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
P+VLNKA+ I+ ++G ++++DE D+ LPYLQNI+SE +SEDC + G+++P+ TI+LTNVWAIHRDP W D +SFKPERFEKEG ++
Sbjct: 319 PDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKEGEAQ 421 HSP 2 Score: 51.6026 bits (122), Expect = 1.823e-36 Identity = 28/72 (38.89%), Postives = 39/72 (54.17%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
++ G+ KLM FG+GRRACPG LA R + L G QC EW K ++ + +P K P +A+C
Sbjct: 415 EKEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSL-IQCLEWEKIGEEVDMSEGKGVTMP--KAKPLEAMC 483 HSP 3 Score: 107.457 bits (267), Expect = 7.230e-28 Identity = 60/121 (49.59%), Postives = 76/121 (62.81%), Query Frame = 1
Query: 55 SLTFIITIKILLQTQSRRLKNLXXXXXXXXXXX-NLHHLKHPLHRTFATLSQKYGD--IVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
S+ F++ I L + +R NL +L LK P+HRTF +LSQ + I SL G+RLV V SS S+ EECF KNDVVLANRP F+ K++ YDYTT+ + SYGDHWRNLR
Sbjct: 9 SILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNLR 129 HSP 4 Score: 35.039 bits (79), Expect = 7.230e-28 Identity = 14/28 (50.00%), Postives = 21/28 (75.00%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLI 491
RI ++++ SN+RL SFL +R DE RL+
Sbjct: 130 RIGSVEIFSNHRLNSFLSIRKDEIRRLV 157
BLAST of BU964318 vs. TAIR peptide
Match: AT4G37340.1 (| Symbols: CYP81D3 | cytochrome P450, family 81, subfamily D, polypeptide 3 | chr4:17564953-17566706 REVERSE LENGTH=500) HSP 1 Score: 117.857 bits (294), Expect = 1.510e-35 Identity = 51/103 (49.51%), Postives = 72/103 (69.90%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
P++L+KA+ I+ +VG N++++E DL LPYLQNI+SE +SEDC +GG+++P+ T++LTN WAIHRDP W D SFKPERFEKEG ++
Sbjct: 320 PQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFEKEGEAQ 422 HSP 2 Score: 50.447 bits (119), Expect = 1.510e-35 Identity = 23/45 (51.11%), Postives = 29/45 (64.44%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++ G+ KL+ FG+GRRACPG LAQR L G QCFEW +
Sbjct: 416 EKEGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSL-IQCFEWER 459 HSP 3 Score: 101.293 bits (251), Expect = 9.285e-22 Identity = 63/127 (49.61%), Postives = 76/127 (59.84%), Query Frame = 1
Query: 37 FYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXX-NLHHLKHPLHRTFATLSQKYGD--IVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
F + LSLTFII + +R NL +L LK PLHR F ++S+ GD I+SL G+RLV V+SS SL EECF KNDVVLANR L K+I Y TT+ + SYGDHWRNLR
Sbjct: 10 FLFISLSLTFIIG-------RIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRNLR 129
BLAST of BU964318 vs. TAIR peptide
Match: AT3G28740.1 (| Symbols: CYP81D1 | Cytochrome P450 superfamily protein | chr3:10788764-10790552 REVERSE LENGTH=509) HSP 1 Score: 113.235 bits (282), Expect = 9.594e-35 Identity = 51/100 (51.00%), Postives = 68/100 (68.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PEVL KAK I+ Q+G +++++EQD+ KLPYLQ+I+SE +SEDC + G++VP+ TIIL N WAIHRDP W + FKPERFEK+G
Sbjct: 328 PEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEKKG 427 HSP 2 Score: 52.373 bits (124), Expect = 9.594e-35 Identity = 26/49 (53.06%), Postives = 33/49 (67.35%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXR 947
+++G+ KLM FGIGRR+CPG LAQR V L G QCFEW + E +
Sbjct: 424 EKKGEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSL-VQCFEWERVEEK 471 HSP 3 Score: 107.457 bits (267), Expect = 1.296e-23 Identity = 62/130 (47.69%), Postives = 79/130 (60.77%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGD--IVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
+S F++ FLSL K+L + R+L +LH LK PLHR F +LS+ + I SL GSRLV V+SS ++ EECF KNDVVLANRP FL GK+I Y+ TT+ +YGD WRNLR
Sbjct: 15 LSLFFFFIFLSL------KLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWRNLR 138
BLAST of BU964318 vs. TAIR peptide
Match: AT4G37320.1 (| Symbols: CYP81D5 | cytochrome P450, family 81, subfamily D, polypeptide 5 | chr4:17559742-17561690 REVERSE LENGTH=495) HSP 1 Score: 105.916 bits (263), Expect = 6.005e-33 Identity = 44/100 (44.00%), Postives = 68/100 (68.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PE+L KA+ I+ ++G +++++E D+ L YLQNI+SE +SS++C + G+++P+ T++LTNVWA+HRDP W + FKPERFEKEG
Sbjct: 320 PEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERFEKEG 419 HSP 2 Score: 48.521 bits (114), Expect = 6.005e-33 Identity = 23/45 (51.11%), Postives = 29/45 (64.44%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++ G+ KLM FG+GRRACPG L +R V L G C Q FEW +
Sbjct: 416 EKEGEARKLMPFGMGRRACPGAELGKRLVSLALG-CLIQSFEWER 459 HSP 3 Score: 25.409 bits (54), Expect = 6.005e-33 Identity = 10/19 (52.63%), Postives = 13/19 (68.42%), Query Frame = 2
Query: 947 EKLDMMEGKGIAMPXKIPI 1003
E +DM EG+GI MP P+
Sbjct: 463 ELVDMTEGEGITMPKATPL 481 HSP 4 Score: 108.997 bits (271), Expect = 4.453e-24 Identity = 64/131 (48.85%), Postives = 85/131 (64.89%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXX-NLHHLKHPLHRTFATLSQKYGD--IVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
++ + SFL LT I+IK+LL T+S R NL +LH LK P+HRTF ++S+ G+ I L G+RLV VISS S+ EECF KNDVVLANRP + K++ Y++T + + SYGDHWRNLR
Sbjct: 3 LTQILLLSFLFLT--ISIKLLL-TKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRNLR 130
BLAST of BU964318 vs. TAIR peptide
Match: AT5G36220.1 (| Symbols: CYP81D1, CYP91A1 | cytochrome p450 81d1 | chr5:14253827-14256015 REVERSE LENGTH=502) HSP 1 Score: 105.145 bits (261), Expect = 7.796e-33 Identity = 47/99 (47.47%), Postives = 66/99 (66.67%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKE 807
P+V++KA+ I+ +VG +++I+E DL +LPYL+NI+ E +SEDC IG +++P+ T +L N WAIHRDP W D SFKPERFEKE
Sbjct: 326 PDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKE 424 HSP 2 Score: 53.1434 bits (126), Expect = 7.796e-33 Identity = 25/38 (65.79%), Postives = 28/38 (73.68%), Query Frame = 3
Query: 822 KLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
KL+AFG+GRRACPG LAQR VGL G QCFEW +
Sbjct: 429 KLLAFGLGRRACPGSGLAQRIVGLALGSL-IQCFEWER 465 HSP 3 Score: 21.1718 bits (43), Expect = 7.796e-33 Identity = 8/18 (44.44%), Postives = 12/18 (66.67%), Query Frame = 2
Query: 950 KLDMMEGKGIAMPXKIPI 1003
++DM EG G +P IP+
Sbjct: 470 EVDMKEGVGNTVPKAIPL 487 HSP 4 Score: 89.7373 bits (221), Expect = 1.317e-22 Identity = 54/128 (42.19%), Postives = 73/128 (57.03%), Query Frame = 1
Query: 43 YSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKY-----GDIVSLWFGSRLVVVISSASLV-EECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
YS SL F+I L+ + + L +L LK P+HRT + S+ G ++SL GSRLV V+SS + EECF KNDVVLANRP+ + GK++ Y+ T + + YGDHWRNLR
Sbjct: 11 YSIFSLIFLIISFKFLKPKKQNLP--PSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHWRNLR 136 HSP 5 Score: 35.039 bits (79), Expect = 1.317e-22 Identity = 17/31 (54.84%), Postives = 21/31 (67.74%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
R+ TI++ S +RL FL VRTDE RLI L
Sbjct: 137 RLCTIEIFSTHRLNCFLYVRTDEVRRLISRL 167
BLAST of BU964318 vs. TAIR peptide
Match: AT4G37330.1 (| Symbols: CYP81D4 | cytochrome P450, family 81, subfamily D, polypeptide 4 | chr4:17562547-17564569 REVERSE LENGTH=492) HSP 1 Score: 108.227 bits (269), Expect = 2.219e-32 Identity = 48/103 (46.60%), Postives = 67/103 (65.05%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PE+L KA+ I+ +VG ++++DE D+ L YLQ+I+ E SSEDC +GG+++P T++LTN WA+HRDP W D FKPERFEKEG +E
Sbjct: 317 PEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEKEGEAE 419 HSP 2 Score: 46.2098 bits (108), Expect = 2.219e-32 Identity = 21/45 (46.67%), Postives = 29/45 (64.44%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++ G+ KL++FG+GRRACPG LA R + G QCFEW +
Sbjct: 413 EKEGEAEKLISFGMGRRACPGAGLAHRLINQALGSL-VQCFEWER 456 HSP 3 Score: 23.483 bits (49), Expect = 2.219e-32 Identity = 9/20 (45.00%), Postives = 13/20 (65.00%), Query Frame = 2
Query: 944 EEKLDMMEGKGIAMPXKIPI 1003
E+ +DM E KG +P IP+
Sbjct: 459 EDFVDMTEDKGATLPKAIPL 478 HSP 4 Score: 112.464 bits (280), Expect = 1.142e-28 Identity = 66/131 (50.38%), Postives = 83/131 (63.36%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXX-NLHHLKHPLHRTFATLSQKYGD--IVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
++ + SFLSL T+ I L T+S+R NL +LH LK PLHRTF +LS+ G+ + L G+RLV VISS S+ EECF KNDVVLANRP+F K++ Y+ T L S SYGDHWRNLR
Sbjct: 3 LNQILILSFLSL---FTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWRNLR 130 HSP 5 Score: 32.7278 bits (73), Expect = 1.142e-28 Identity = 15/31 (48.39%), Postives = 20/31 (64.52%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RI +++ S +RL SFL +R DE RLI L
Sbjct: 131 RIAAVEIFSTHRLNSFLYIRKDEIRRLISHL 161
BLAST of BU964318 vs. TAIR peptide
Match: AT4G37310.1 (| Symbols: CYP81H1 | cytochrome P450, family 81, subfamily H, polypeptide 1 | chr4:17556152-17558833 REVERSE LENGTH=518) HSP 1 Score: 98.9821 bits (245), Expect = 9.892e-31 Identity = 45/100 (45.00%), Postives = 62/100 (62.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQV---GKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFE 801
P++L + I+TQ+ +++ E+DL + YL+N++SE S DC IGGFNVP+DTI+L N+WAIHRDP+ W D SFKPERFE
Sbjct: 325 PQILETTRQNIDTQMETSSSRRLLKEEDLVNMNYLKNVVSETLRLYPVAPLMVPHVPSSDCVIGGFNVPRDTIVLVNLWAIHRDPSVWDDPTSFKPERFE 424 HSP 2 Score: 53.1434 bits (126), Expect = 9.892e-31 Identity = 25/36 (69.44%), Postives = 27/36 (75.00%), Query Frame = 3
Query: 822 KLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
K+M FG+GRRACPGLSLA R VGL G QCFEW
Sbjct: 435 KMMPFGLGRRACPGLSLANRVVGLLLG-SMIQCFEW 469 HSP 3 Score: 108.612 bits (270), Expect = 7.745e-27 Identity = 60/127 (47.24%), Postives = 78/127 (61.42%), Query Frame = 1
Query: 34 LFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPL-HRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFI-KNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
L + L FI+ I +L+ ++ L +LH LK PL HRT + LS G + SL GSRL V+ISS + EECF+ KND+VLANRPRF+ GKY+ YDYT++ + YGDHWRNLR
Sbjct: 5 LLILTTLVAIFIVKIVLLVTKPNKNLP--PSPNICFPIIGHLHLLKKPLLHRTLSHLSHSLGPVFSLRLGSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAPYGDHWRNLR 129 HSP 4 Score: 30.4166 bits (67), Expect = 7.745e-27 Identity = 15/35 (42.86%), Postives = 21/35 (60.00%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL*SMS 512
RIT ++V S NRL + +R DE L+Q L +S
Sbjct: 130 RITALEVFSTNRLNASAEIRHDEVKMLLQKLHDLS 164
BLAST of BU964318 vs. TAIR peptide
Match: AT4G37360.1 (| Symbols: CYP81D2 | cytochrome P450, family 81, subfamily D, polypeptide 2 | chr4:17567124-17568858 REVERSE LENGTH=499) HSP 1 Score: 119.013 bits (297), Expect = 3.542e-38 Identity = 51/100 (51.00%), Postives = 70/100 (70.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PEVLNKA+ I+ +G +++++E D+P LPYLQNI+SE +S+DC +GG+++P+ T++LTN WAIHRDP W D SFKPERFEKEG
Sbjct: 320 PEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKEG 419 HSP 2 Score: 52.373 bits (124), Expect = 3.542e-38 Identity = 25/45 (55.56%), Postives = 31/45 (68.89%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++ G+ KLM FG+GRRACPG LAQR V L+ G QCFEW +
Sbjct: 416 EKEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSL-IQCFEWER 459 HSP 3 Score: 26.1794 bits (56), Expect = 3.542e-38 Identity = 10/20 (50.00%), Postives = 14/20 (70.00%), Query Frame = 2
Query: 944 EEKLDMMEGKGIAMPXKIPI 1003
EE++DM EG G+ MP P+
Sbjct: 462 EEEVDMTEGPGLTMPKARPL 481 HSP 4 Score: 94.3597 bits (233), Expect = 1.135e-19 Identity = 54/127 (42.52%), Postives = 75/127 (59.06%), Query Frame = 1
Query: 34 LFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGD--IVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
+F + F+ L+ I I + R+L +L LK PLHR F ++SQ GD I+SL G+RL+ V+SS S+ EECF KNDV+LANR ++ K+I Y +T+ S SY +HWRNLR
Sbjct: 7 IFTFCFIVLSLIFLIGRI----KRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRNLR 129
BLAST of BU964318 vs. TAIR peptide
Match: AT1G66540.1 (| Symbols: | Cytochrome P450 superfamily protein | chr1:24824837-24826502 FORWARD LENGTH=386) HSP 1 Score: 118.627 bits (296), Expect = 3.564e-38 Identity = 63/148 (42.57%), Postives = 86/148 (58.11%), Query Frame = 1
Query: 388 DHWRNLRV*QPLMFSLTTV----LPLFWEFGQTKQIDLYKYYKAC---PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
DH +L+V QP ++ T+ L L T + L + PEVL K + I+ Q+G +++++E D+P LPYLQNI+SE SSEDC +GG+++P T++L NVWAIHRDP W D SFKPERFEKEG
Sbjct: 159 DHLLSLQVSQPEYYTDHTIKGTMLSLILAGTDTSAVTLEWALSSLLNNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVSETLRLYPAGPLLVPHISSEDCKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPERFEKEG 306 HSP 2 Score: 50.447 bits (119), Expect = 3.564e-38 Identity = 23/45 (51.11%), Postives = 31/45 (68.89%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++ G+ KL+ FG+GRRACPG LA+R V L+ G QCFEW +
Sbjct: 303 EKEGETHKLLTFGLGRRACPGSGLARRLVSLSLGSL-IQCFEWER 346 HSP 3 Score: 28.4906 bits (62), Expect = 3.564e-38 Identity = 11/20 (55.00%), Postives = 15/20 (75.00%), Query Frame = 2
Query: 944 EEKLDMMEGKGIAMPXKIPI 1003
EE++DM EG G+ MP IP+
Sbjct: 349 EEEVDMTEGGGLTMPRAIPL 368
BLAST of BU964318 vs. TAIR peptide
Match: AT1G66540.2 (| Symbols: | Cytochrome P450 superfamily protein | chr1:24825446-24826502 FORWARD LENGTH=327) HSP 1 Score: 118.627 bits (296), Expect = 3.579e-38 Identity = 63/148 (42.57%), Postives = 86/148 (58.11%), Query Frame = 1
Query: 388 DHWRNLRV*QPLMFSLTTV----LPLFWEFGQTKQIDLYKYYKAC---PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
DH +L+V QP ++ T+ L L T + L + PEVL K + I+ Q+G +++++E D+P LPYLQNI+SE SSEDC +GG+++P T++L NVWAIHRDP W D SFKPERFEKEG
Sbjct: 100 DHLLSLQVSQPEYYTDHTIKGTMLSLILAGTDTSAVTLEWALSSLLNNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVSETLRLYPAGPLLVPHISSEDCKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPERFEKEG 247 HSP 2 Score: 50.447 bits (119), Expect = 3.579e-38 Identity = 23/45 (51.11%), Postives = 31/45 (68.89%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++ G+ KL+ FG+GRRACPG LA+R V L+ G QCFEW +
Sbjct: 244 EKEGETHKLLTFGLGRRACPGSGLARRLVSLSLGSL-IQCFEWER 287 HSP 3 Score: 28.4906 bits (62), Expect = 3.579e-38 Identity = 11/20 (55.00%), Postives = 15/20 (75.00%), Query Frame = 2
Query: 944 EEKLDMMEGKGIAMPXKIPI 1003
EE++DM EG G+ MP IP+
Sbjct: 290 EEEVDMTEGGGLTMPRAIPL 309
BLAST of BU964318 vs. TAIR peptide
Match: AT4G37370.1 (| Symbols: CYP81D8 | cytochrome P450, family 81, subfamily D, polypeptide 8 | chr4:17569971-17571678 REVERSE LENGTH=497) HSP 1 Score: 119.783 bits (299), Expect = 1.823e-36 Identity = 52/103 (50.49%), Postives = 73/103 (70.87%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
P+VLNKA+ I+ ++G ++++DE D+ LPYLQNI+SE +SEDC + G+++P+ TI+LTNVWAIHRDP W D +SFKPERFEKEG ++
Sbjct: 319 PDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKEGEAQ 421 HSP 2 Score: 51.6026 bits (122), Expect = 1.823e-36 Identity = 28/72 (38.89%), Postives = 39/72 (54.17%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
++ G+ KLM FG+GRRACPG LA R + L G QC EW K ++ + +P K P +A+C
Sbjct: 415 EKEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSL-IQCLEWEKIGEEVDMSEGKGVTMP--KAKPLEAMC 483 HSP 3 Score: 107.457 bits (267), Expect = 7.230e-28 Identity = 60/121 (49.59%), Postives = 76/121 (62.81%), Query Frame = 1
Query: 55 SLTFIITIKILLQTQSRRLKNLXXXXXXXXXXX-NLHHLKHPLHRTFATLSQKYGD--IVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
S+ F++ I L + +R NL +L LK P+HRTF +LSQ + I SL G+RLV V SS S+ EECF KNDVVLANRP F+ K++ YDYTT+ + SYGDHWRNLR
Sbjct: 9 SILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNLR 129 HSP 4 Score: 35.039 bits (79), Expect = 7.230e-28 Identity = 14/28 (50.00%), Postives = 21/28 (75.00%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLI 491
RI ++++ SN+RL SFL +R DE RL+
Sbjct: 130 RIGSVEIFSNHRLNSFLSIRKDEIRRLV 157
BLAST of BU964318 vs. TAIR peptide
Match: AT4G37340.1 (| Symbols: CYP81D3 | cytochrome P450, family 81, subfamily D, polypeptide 3 | chr4:17564953-17566706 REVERSE LENGTH=500) HSP 1 Score: 117.857 bits (294), Expect = 1.510e-35 Identity = 51/103 (49.51%), Postives = 72/103 (69.90%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
P++L+KA+ I+ +VG N++++E DL LPYLQNI+SE +SEDC +GG+++P+ T++LTN WAIHRDP W D SFKPERFEKEG ++
Sbjct: 320 PQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFEKEGEAQ 422 HSP 2 Score: 50.447 bits (119), Expect = 1.510e-35 Identity = 23/45 (51.11%), Postives = 29/45 (64.44%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++ G+ KL+ FG+GRRACPG LAQR L G QCFEW +
Sbjct: 416 EKEGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSL-IQCFEWER 459 HSP 3 Score: 101.293 bits (251), Expect = 9.285e-22 Identity = 63/127 (49.61%), Postives = 76/127 (59.84%), Query Frame = 1
Query: 37 FYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXX-NLHHLKHPLHRTFATLSQKYGD--IVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
F + LSLTFII + +R NL +L LK PLHR F ++S+ GD I+SL G+RLV V+SS SL EECF KNDVVLANR L K+I Y TT+ + SYGDHWRNLR
Sbjct: 10 FLFISLSLTFIIG-------RIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRNLR 129
BLAST of BU964318 vs. TAIR peptide
Match: AT3G28740.1 (| Symbols: CYP81D1 | Cytochrome P450 superfamily protein | chr3:10788764-10790552 REVERSE LENGTH=509) HSP 1 Score: 113.235 bits (282), Expect = 9.594e-35 Identity = 51/100 (51.00%), Postives = 68/100 (68.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PEVL KAK I+ Q+G +++++EQD+ KLPYLQ+I+SE +SEDC + G++VP+ TIIL N WAIHRDP W + FKPERFEK+G
Sbjct: 328 PEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEKKG 427 HSP 2 Score: 52.373 bits (124), Expect = 9.594e-35 Identity = 26/49 (53.06%), Postives = 33/49 (67.35%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXR 947
+++G+ KLM FGIGRR+CPG LAQR V L G QCFEW + E +
Sbjct: 424 EKKGEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSL-VQCFEWERVEEK 471 HSP 3 Score: 107.457 bits (267), Expect = 1.296e-23 Identity = 62/130 (47.69%), Postives = 79/130 (60.77%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGD--IVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
+S F++ FLSL K+L + R+L +LH LK PLHR F +LS+ + I SL GSRLV V+SS ++ EECF KNDVVLANRP FL GK+I Y+ TT+ +YGD WRNLR
Sbjct: 15 LSLFFFFIFLSL------KLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWRNLR 138
BLAST of BU964318 vs. TAIR peptide
Match: AT4G37320.1 (| Symbols: CYP81D5 | cytochrome P450, family 81, subfamily D, polypeptide 5 | chr4:17559742-17561690 REVERSE LENGTH=495) HSP 1 Score: 105.916 bits (263), Expect = 6.005e-33 Identity = 44/100 (44.00%), Postives = 68/100 (68.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PE+L KA+ I+ ++G +++++E D+ L YLQNI+SE +SS++C + G+++P+ T++LTNVWA+HRDP W + FKPERFEKEG
Sbjct: 320 PEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERFEKEG 419 HSP 2 Score: 48.521 bits (114), Expect = 6.005e-33 Identity = 23/45 (51.11%), Postives = 29/45 (64.44%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++ G+ KLM FG+GRRACPG L +R V L G C Q FEW +
Sbjct: 416 EKEGEARKLMPFGMGRRACPGAELGKRLVSLALG-CLIQSFEWER 459 HSP 3 Score: 25.409 bits (54), Expect = 6.005e-33 Identity = 10/19 (52.63%), Postives = 13/19 (68.42%), Query Frame = 2
Query: 947 EKLDMMEGKGIAMPXKIPI 1003
E +DM EG+GI MP P+
Sbjct: 463 ELVDMTEGEGITMPKATPL 481 HSP 4 Score: 108.997 bits (271), Expect = 4.453e-24 Identity = 64/131 (48.85%), Postives = 85/131 (64.89%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXX-NLHHLKHPLHRTFATLSQKYGD--IVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
++ + SFL LT I+IK+LL T+S R NL +LH LK P+HRTF ++S+ G+ I L G+RLV VISS S+ EECF KNDVVLANRP + K++ Y++T + + SYGDHWRNLR
Sbjct: 3 LTQILLLSFLFLT--ISIKLLL-TKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRNLR 130
BLAST of BU964318 vs. TAIR peptide
Match: AT5G36220.1 (| Symbols: CYP81D1, CYP91A1 | cytochrome p450 81d1 | chr5:14253827-14256015 REVERSE LENGTH=502) HSP 1 Score: 105.145 bits (261), Expect = 7.796e-33 Identity = 47/99 (47.47%), Postives = 66/99 (66.67%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKE 807
P+V++KA+ I+ +VG +++I+E DL +LPYL+NI+ E +SEDC IG +++P+ T +L N WAIHRDP W D SFKPERFEKE
Sbjct: 326 PDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKE 424 HSP 2 Score: 53.1434 bits (126), Expect = 7.796e-33 Identity = 25/38 (65.79%), Postives = 28/38 (73.68%), Query Frame = 3
Query: 822 KLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
KL+AFG+GRRACPG LAQR VGL G QCFEW +
Sbjct: 429 KLLAFGLGRRACPGSGLAQRIVGLALGSL-IQCFEWER 465 HSP 3 Score: 21.1718 bits (43), Expect = 7.796e-33 Identity = 8/18 (44.44%), Postives = 12/18 (66.67%), Query Frame = 2
Query: 950 KLDMMEGKGIAMPXKIPI 1003
++DM EG G +P IP+
Sbjct: 470 EVDMKEGVGNTVPKAIPL 487 HSP 4 Score: 89.7373 bits (221), Expect = 1.317e-22 Identity = 54/128 (42.19%), Postives = 73/128 (57.03%), Query Frame = 1
Query: 43 YSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKY-----GDIVSLWFGSRLVVVISSASLV-EECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
YS SL F+I L+ + + L +L LK P+HRT + S+ G ++SL GSRLV V+SS + EECF KNDVVLANRP+ + GK++ Y+ T + + YGDHWRNLR
Sbjct: 11 YSIFSLIFLIISFKFLKPKKQNLP--PSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHWRNLR 136 HSP 5 Score: 35.039 bits (79), Expect = 1.317e-22 Identity = 17/31 (54.84%), Postives = 21/31 (67.74%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
R+ TI++ S +RL FL VRTDE RLI L
Sbjct: 137 RLCTIEIFSTHRLNCFLYVRTDEVRRLISRL 167
BLAST of BU964318 vs. TAIR peptide
Match: AT4G37330.1 (| Symbols: CYP81D4 | cytochrome P450, family 81, subfamily D, polypeptide 4 | chr4:17562547-17564569 REVERSE LENGTH=492) HSP 1 Score: 108.227 bits (269), Expect = 2.219e-32 Identity = 48/103 (46.60%), Postives = 67/103 (65.05%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PE+L KA+ I+ +VG ++++DE D+ L YLQ+I+ E SSEDC +GG+++P T++LTN WA+HRDP W D FKPERFEKEG +E
Sbjct: 317 PEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEKEGEAE 419 HSP 2 Score: 46.2098 bits (108), Expect = 2.219e-32 Identity = 21/45 (46.67%), Postives = 29/45 (64.44%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++ G+ KL++FG+GRRACPG LA R + G QCFEW +
Sbjct: 413 EKEGEAEKLISFGMGRRACPGAGLAHRLINQALGSL-VQCFEWER 456 HSP 3 Score: 23.483 bits (49), Expect = 2.219e-32 Identity = 9/20 (45.00%), Postives = 13/20 (65.00%), Query Frame = 2
Query: 944 EEKLDMMEGKGIAMPXKIPI 1003
E+ +DM E KG +P IP+
Sbjct: 459 EDFVDMTEDKGATLPKAIPL 478 HSP 4 Score: 112.464 bits (280), Expect = 1.142e-28 Identity = 66/131 (50.38%), Postives = 83/131 (63.36%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXX-NLHHLKHPLHRTFATLSQKYGD--IVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
++ + SFLSL T+ I L T+S+R NL +LH LK PLHRTF +LS+ G+ + L G+RLV VISS S+ EECF KNDVVLANRP+F K++ Y+ T L S SYGDHWRNLR
Sbjct: 3 LNQILILSFLSL---FTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWRNLR 130 HSP 5 Score: 32.7278 bits (73), Expect = 1.142e-28 Identity = 15/31 (48.39%), Postives = 20/31 (64.52%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RI +++ S +RL SFL +R DE RLI L
Sbjct: 131 RIAAVEIFSTHRLNSFLYIRKDEIRRLISHL 161
BLAST of BU964318 vs. TAIR peptide
Match: AT4G37310.1 (| Symbols: CYP81H1 | cytochrome P450, family 81, subfamily H, polypeptide 1 | chr4:17556152-17558833 REVERSE LENGTH=518) HSP 1 Score: 98.9821 bits (245), Expect = 9.892e-31 Identity = 45/100 (45.00%), Postives = 62/100 (62.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQV---GKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFE 801
P++L + I+TQ+ +++ E+DL + YL+N++SE S DC IGGFNVP+DTI+L N+WAIHRDP+ W D SFKPERFE
Sbjct: 325 PQILETTRQNIDTQMETSSSRRLLKEEDLVNMNYLKNVVSETLRLYPVAPLMVPHVPSSDCVIGGFNVPRDTIVLVNLWAIHRDPSVWDDPTSFKPERFE 424 HSP 2 Score: 53.1434 bits (126), Expect = 9.892e-31 Identity = 25/36 (69.44%), Postives = 27/36 (75.00%), Query Frame = 3
Query: 822 KLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
K+M FG+GRRACPGLSLA R VGL G QCFEW
Sbjct: 435 KMMPFGLGRRACPGLSLANRVVGLLLG-SMIQCFEW 469 HSP 3 Score: 108.612 bits (270), Expect = 7.745e-27 Identity = 60/127 (47.24%), Postives = 78/127 (61.42%), Query Frame = 1
Query: 34 LFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPL-HRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFI-KNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
L + L FI+ I +L+ ++ L +LH LK PL HRT + LS G + SL GSRL V+ISS + EECF+ KND+VLANRPRF+ GKY+ YDYT++ + YGDHWRNLR
Sbjct: 5 LLILTTLVAIFIVKIVLLVTKPNKNLP--PSPNICFPIIGHLHLLKKPLLHRTLSHLSHSLGPVFSLRLGSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAPYGDHWRNLR 129 HSP 4 Score: 30.4166 bits (67), Expect = 7.745e-27 Identity = 15/35 (42.86%), Postives = 21/35 (60.00%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL*SMS 512
RIT ++V S NRL + +R DE L+Q L +S
Sbjct: 130 RITALEVFSTNRLNASAEIRHDEVKMLLQKLHDLS 164
BLAST of BU964318 vs. TrEMBL
Match: Q6YA92_PEA (Cytochrome P450 OS=Pisum sativum PE=2 SV=1) HSP 1 Score: 234.958 bits (598), Expect = 1.213e-70 Identity = 117/128 (91.41%), Postives = 117/128 (91.41%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
MSPLFYYSFLSLTFIITIKILLQTQSRRLKNL NLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR
Sbjct: 1 MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 128 HSP 2 Score: 58.5362 bits (140), Expect = 1.213e-70 Identity = 29/29 (100.00%), Postives = 29/29 (100.00%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQ 494
RITTIDVLSNNRLTSFLGVRTDEANRLIQ
Sbjct: 129 RITTIDVLSNNRLTSFLGVRTDEANRLIQ 157 HSP 3 Score: 170.244 bits (430), Expect = 4.175e-68 Identity = 84/100 (84.00%), Postives = 84/100 (84.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PEVLNKAK IETQVGKNKIIDEQDLPKLPYLQNIISE YSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG
Sbjct: 324 PEVLNKAKEEIETQVGKNKIIDEQDLPKLPYLQNIISEALKLHPPAPLLLPHYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 423 HSP 4 Score: 114.775 bits (286), Expect = 4.175e-68 Identity = 55/72 (76.39%), Postives = 58/72 (80.56%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
++ G+ KLMAFGIGRRACPGLSLAQRTVGLNCG CWFQCFEW KRE L KVLPCLKRSPFKALC
Sbjct: 420 EKEGEVNKLMAFGIGRRACPGLSLAQRTVGLNCGACWFQCFEW-KRESEEKLDMMEGKVLPCLKRSPFKALC 490
BLAST of BU964318 vs. TrEMBL
Match: Q6WNQ9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula PE=2 SV=1) HSP 1 Score: 201.06 bits (510), Expect = 6.945e-59 Identity = 97/125 (77.60%), Postives = 103/125 (82.40%), Query Frame = 1
Query: 34 LFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
LFYYS LSL+FIITIKILL+ SRRLKNL NLHHLKHPLHRTF TLSQ YGDI SLWFGSRLVVV+SS SL ECF KND++LANRPRFLTGKYIFY+YTTLGS SYGDHWRNLR
Sbjct: 2 LFYYSLLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLR 126 HSP 2 Score: 53.1434 bits (126), Expect = 6.945e-59 Identity = 27/31 (87.10%), Postives = 27/31 (87.10%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RITTIDVLSNNRL SFLGVR DE NRLIQ L
Sbjct: 127 RITTIDVLSNNRLNSFLGVRRDETNRLIQKL 157 HSP 3 Score: 149.058 bits (375), Expect = 2.464e-46 Identity = 71/99 (71.72%), Postives = 77/99 (77.78%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKE 807
PEVL KAK ++TQ+GKNK++DEQDL KLPYLQNIISE YSSEDCTIG FNVPKDTIILTNVW IHRDP W+DALSFKPERFEKE
Sbjct: 325 PEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKE 423 HSP 4 Score: 63.1586 bits (152), Expect = 2.464e-46 Identity = 34/65 (52.31%), Postives = 41/65 (63.08%), Query Frame = 3
Query: 822 KLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
K+MAFG+GRRACPGLSLAQRTVG G QCFEW +RE L K + + P +A+C
Sbjct: 428 KVMAFGLGRRACPGLSLAQRTVGFTVGLL-IQCFEW-ERESEEKLDMMEGKGITMPMKIPLRAMC 490
BLAST of BU964318 vs. TrEMBL
Match: Q2ENF7_ASTMO (Isoflavone 3'-hydroxylase (Fragment) OS=Astragalus mongholicus PE=2 SV=1) HSP 1 Score: 164.081 bits (414), Expect = 5.965e-45 Identity = 73/85 (85.88%), Postives = 80/85 (94.12%), Query Frame = 1
Query: 154 NLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
NLHHLKHPLHRTF TLSQKYGDI+SLWFGSRLVVV++S S+ +ECF KNDVVLANRP+FLTGKYIFY+YTTLGS SYGDHWRNLR
Sbjct: 2 NLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLR 86 HSP 2 Score: 43.5134 bits (101), Expect = 5.965e-45 Identity = 22/31 (70.97%), Postives = 25/31 (80.65%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RITTIDVLSN+RL SF GVR DE RL++ L
Sbjct: 87 RITTIDVLSNHRLNSFHGVRKDETLRLVEKL 117
BLAST of BU964318 vs. TrEMBL
Match: Q9ZRW6_CICAR (Cytochrome P450 OS=Cicer arietinum GN=cyp81E3 PE=2 SV=1) HSP 1 Score: 162.925 bits (411), Expect = 1.420e-43 Identity = 80/125 (64.00%), Postives = 92/125 (73.60%), Query Frame = 1
Query: 34 LFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
L YS L+F I++L Q SR+ KNL NLHHLK PLHRTF LS+ YGDI+SLWFGSRLVVV+SS S ++CF KNDVVLANRPRFL+GKYIFY+YTTLGS SY +HWRNLR
Sbjct: 6 LLCYSLFYLSFFFIIRLLFQ--SRKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSKTYGDIISLWFGSRLVVVVSSLSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYSEHWRNLR 128 HSP 2 Score: 40.0466 bits (92), Expect = 1.420e-43 Identity = 18/28 (64.29%), Postives = 23/28 (82.14%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLI 491
RIT++DVLSN+R+ +F GVR DE RLI
Sbjct: 129 RITSLDVLSNHRINNFSGVRRDETQRLI 156 HSP 3 Score: 117.472 bits (293), Expect = 1.177e-31 Identity = 52/103 (50.49%), Postives = 70/103 (67.96%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PE+L K K ++ VG+++++DE DLPKL YL+N+I E +S+DC IGG+ VP+DTI+L N WAIHRDP WS+A SFKPERF+K+G E
Sbjct: 322 PEILKKVKDELDIHVGQDRLVDESDLPKLTYLKNVIYETLRLCTPAPLLLPHSTSDDCIIGGYKVPRDTIVLINAWAIHRDPKSWSEATSFKPERFDKKGEIE 424 HSP 4 Score: 45.0542 bits (105), Expect = 1.177e-31 Identity = 19/44 (43.18%), Postives = 30/44 (68.18%), Query Frame = 3
Query: 804 RRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++G+ K++AFG+GRR CPG +LA RT+ + QCF+W +
Sbjct: 419 KKGEIEKVIAFGMGRRVCPGEALALRTISMTLALL-VQCFDWKR 461 HSP 5 Score: 20.7866 bits (42), Expect = 1.177e-31 Identity = 8/24 (33.33%), Postives = 13/24 (54.17%), Query Frame = 2
Query: 932 KARXEEKLDMMEGKGIAMPXKIPI 1003
K ++K+DM E G + IP+
Sbjct: 460 KRTSDDKIDMAERDGFVLTKLIPL 483
BLAST of BU964318 vs. TrEMBL
Match: Q9MBE4_LOTJA (Cytochrome P450 OS=Lotus japonicus PE=2 SV=1) HSP 1 Score: 162.925 bits (411), Expect = 4.094e-43 Identity = 79/123 (64.23%), Postives = 91/123 (73.98%), Query Frame = 1
Query: 40 YYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
YYS + IK+ L SR+ KNL NLHHLK PLHRTF LS+KYGD+ SLWFG+RLVVV+SS + V+ECF KNDVVLANRPRFL+GKYIFY+YTTLGS SYG+HWRNLR
Sbjct: 8 YYSLFYVALFAIIKLFLG--SRKFKNLPPGPTSLPIIGNLHHLKRPLHRTFRALSEKYGDVFSLWFGNRLVVVVSSFADVQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 128 HSP 2 Score: 38.5058 bits (88), Expect = 4.094e-43 Identity = 18/31 (58.06%), Postives = 24/31 (77.42%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RIT++DVLSN+R+ SF +R DE RLI+ L
Sbjct: 129 RITSLDVLSNHRINSFSPIRRDETTRLIRKL 159 HSP 3 Score: 119.013 bits (297), Expect = 5.679e-32 Identity = 51/103 (49.51%), Postives = 73/103 (70.87%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PEVL K K ++T VG+++++DE D+PKL YL+N+I+E +S+DCTIGG+ VP+DTI+L N WA+HRDP W++A +FKPERF+K+G E
Sbjct: 323 PEVLEKIKAELDTHVGQDRLVDESDIPKLTYLKNVINETLRLYTPAPLLLPHSASDDCTIGGYKVPRDTIVLINAWALHRDPQLWTEATTFKPERFDKKGELE 425 HSP 4 Score: 45.0542 bits (105), Expect = 5.679e-32 Identity = 26/71 (36.62%), Postives = 37/71 (52.11%), Query Frame = 3
Query: 804 RRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
++G+ KL+ FG+GRRACPG LA R + + QCF+W KR + +K P KA+C
Sbjct: 420 KKGELEKLIPFGLGRRACPGELLAIRAISMTLALL-IQCFDW-KRVSDEEIDMGERDGFVLMKSIPVKAMC 488
BLAST of BU964318 vs. TrEMBL
Match: Q9ZWF2_GLYEC (Cytochrome P450 OS=Glycyrrhiza echinata GN=CYP Ge-31 PE=2 SV=1) HSP 1 Score: 160.614 bits (405), Expect = 5.335e-43 Identity = 78/120 (65.00%), Postives = 92/120 (76.67%), Query Frame = 1
Query: 49 FLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
+L+L FI I I ++R+ KNL NLHHLK PLHRTF LS+KYG ++SLWFGSRLVVV+SSAS ++CF KNDVVLANRPRFL+GKYIFY+YTTLGS SYG+HWRNLR
Sbjct: 13 YLALFFIFNIVI----RARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVISLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 128 HSP 2 Score: 40.4318 bits (93), Expect = 5.335e-43 Identity = 18/28 (64.29%), Postives = 22/28 (78.57%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLI 491
RIT +DVLSN+R+ SF G+R DE RLI
Sbjct: 129 RITALDVLSNHRINSFSGIRRDETQRLI 156 HSP 3 Score: 118.242 bits (295), Expect = 7.396e-32 Identity = 52/103 (50.49%), Postives = 72/103 (69.90%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PEVL K K ++T VG+++++DE DLPKL YL+N+I+E +S++C IGG+ VP+DTI+L N WAIHRDP W++A +FKPERFEK+G E
Sbjct: 322 PEVLKKVKDELDTHVGQDRLVDESDLPKLSYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGELE 424 HSP 4 Score: 45.4394 bits (106), Expect = 7.396e-32 Identity = 20/43 (46.51%), Postives = 29/43 (67.44%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
+++G+ KL+AFG+GRRACPG LA R + + QCF+W
Sbjct: 418 EKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALL-IQCFDW 459
BLAST of BU964318 vs. TrEMBL
Match: Q6WNR0_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula PE=2 SV=1) HSP 1 Score: 160.614 bits (405), Expect = 3.404e-42 Identity = 77/122 (63.11%), Postives = 92/122 (75.41%), Query Frame = 1
Query: 43 YSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
YS L+ I++L Q SR+ KNL NLHHLK PL+RTF L++KYG+++SLWFGSRLVVV+SS S +ECF KNDVVLANRPRFL+GKYIFY+YTTLGS SYG+HWRNLR
Sbjct: 9 YSLFYLSIFFIIRLLFQ--SRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 128 HSP 2 Score: 37.7354 bits (86), Expect = 3.404e-42 Identity = 17/31 (54.84%), Postives = 24/31 (77.42%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RIT++DVLSN+R+ +F +R DE RLI+ L
Sbjct: 129 RITSLDVLSNHRINNFAPIRRDETQRLIKKL 159 HSP 3 Score: 114.39 bits (285), Expect = 1.351e-30 Identity = 49/103 (47.57%), Postives = 71/103 (68.93%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PEVL K + ++T VG+++++DE DLPKL YL+N+I E ++++C +GG+ VP+DTI+L N WAIHRDP WS+A +FKPERF+K+G E
Sbjct: 322 PEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDKKGELE 424 HSP 4 Score: 45.0542 bits (105), Expect = 1.351e-30 Identity = 26/71 (36.62%), Postives = 37/71 (52.11%), Query Frame = 3
Query: 804 RRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
++G+ K++AFG+GRRACPG LA R + + QCF+W KR + K P KA+C
Sbjct: 419 KKGELEKMIAFGMGRRACPGEGLALRAISMTLALL-VQCFDW-KRINDEKIDMSERDGFTMTKLLPLKAMC 487
BLAST of BU964318 vs. TrEMBL
Match: Q9XFX0_CICAR (Cytochrome P450 monooxygenase OS=Cicer arietinum GN=cyp81E3v2 PE=2 SV=1) HSP 1 Score: 158.303 bits (399), Expect = 3.404e-42 Identity = 77/124 (62.10%), Postives = 89/124 (71.77%), Query Frame = 1
Query: 37 FYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
F YS L+ ++L Q SR+ KNL N HHLK PLHRTF LS+ YGDI+SLWFGSRLVVV+SS S ++CF KNDVVLANRPRFL+GKYIFY+YTTLGS SY +HWRNLR
Sbjct: 7 FAYSLFYLSIFFIFRLLFQ--SRKFKNLPPGPPSLPIIGNFHHLKRPLHRTFKGLSKTYGDIISLWFGSRLVVVVSSLSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYSEHWRNLR 128 HSP 2 Score: 40.0466 bits (92), Expect = 3.404e-42 Identity = 18/28 (64.29%), Postives = 23/28 (82.14%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLI 491
RIT++DVLSN+R+ +F GVR DE RLI
Sbjct: 129 RITSLDVLSNHRINNFSGVRRDETQRLI 156 HSP 3 Score: 117.857 bits (294), Expect = 9.632e-32 Identity = 52/103 (50.49%), Postives = 71/103 (68.93%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PEVL K + ++T VG+++++DE DLPKL YL+N+I E +S+DC IGG+ VP+DTI+L N WAIHRDP WS+A +FKPERF+K+G E
Sbjct: 322 PEVLKKVRDELDTHVGQDRLVDESDLPKLTYLKNVIYETLRLCTPAPLLLPHSTSDDCIIGGYKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKGEIE 424 HSP 4 Score: 45.4394 bits (106), Expect = 9.632e-32 Identity = 19/44 (43.18%), Postives = 30/44 (68.18%), Query Frame = 3
Query: 804 RRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++G+ K++AFG+GRR CPG +LA RT+ + QCF+W +
Sbjct: 419 KKGEIEKVIAFGMGRRVCPGEALALRTISMTLALL-IQCFDWKR 461
BLAST of BU964318 vs. TrEMBL
Match: Q9SML1_CICAR (Cytochrome P450 monooxygenase (Fragment) OS=Cicer arietinum GN=cyp81E4 PE=2 SV=1) HSP 1 Score: 132.109 bits (331), Expect = 8.275e-41 Identity = 64/100 (64.00%), Postives = 74/100 (74.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
P+VL ++K TQ+GK+K+I+E DL KLPYLQNII+E YSSED T+GGFNVPKDTIILTNVWAIHRDP WSDA SFKPERFE +G
Sbjct: 31 PDVLKQSKRRNRTQIGKDKLIEEHDLSKLPYLQNIINETLRLHPPAPLSLPHYSSEDFTLGGFNVPKDTIILTNVWAIHRDPKYWSDASSFKPERFEIDG 130 HSP 2 Score: 61.6178 bits (148), Expect = 8.275e-41 Identity = 36/69 (52.17%), Postives = 41/69 (59.42%), Query Frame = 3
Query: 810 GK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
G+ KL+AFG+GRRACPG SLA RTVGL G QCFEW KRE + K L P KA+C
Sbjct: 130 GELNKLIAFGLGRRACPGQSLAHRTVGLTLGLL-IQCFEW-KRETEEKIDLREGKGLTMPMGVPLKAMC 196
BLAST of BU964318 vs. TrEMBL
Match: Q6WNQ8_MEDTR (CYP81E8 OS=Medicago truncatula PE=2 SV=1) HSP 1 Score: 149.828 bits (377), Expect = 1.244e-38 Identity = 76/126 (60.32%), Postives = 87/126 (69.05%), Query Frame = 1
Query: 37 FYYSFLSLTF--IITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
FY S + F IIT+K+ T SR+ KNL NLH LK PLH TF TLSQKYG I SLWFGSRLVVV+SS ++ +ECF KND+VLANRP FLTGKYI Y+ TT+ YGDHWRNLR
Sbjct: 4 FYLSLIISLFFLIITLKVFFNT-SRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLR 128 HSP 2 Score: 36.5798 bits (83), Expect = 1.244e-38 Identity = 18/31 (58.06%), Postives = 24/31 (77.42%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RI +I++LS++RL SFL +R DE RLIQ L
Sbjct: 129 RILSIEILSSHRLNSFLEIRRDEIMRLIQKL 159 HSP 3 Score: 112.079 bits (279), Expect = 3.105e-33 Identity = 48/100 (48.00%), Postives = 67/100 (67.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PE++ KAK ++T +G ++ +DE D+ KLPYLQ+I+ E SSED ++GG+N+P++TI++ N W IHRDP WSD FKPERFEKEG
Sbjct: 322 PEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKEG 421 HSP 4 Score: 56.225 bits (134), Expect = 3.105e-33 Identity = 30/72 (41.67%), Postives = 43/72 (59.72%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
++ G+ KL++FG+GRRACPG +L+QRT GL G QCFEW KR + K + K++ A+C
Sbjct: 418 EKEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLL-IQCFEW-KRIGEEKIDMVEAKGITAGKKTSLNAMC 487
BLAST of BU964318 vs. Lotus protein
Match: LjSGA_054441.1 (+ phase: 0 /partial) HSP 1 Score: 186.422 bits (472), Expect = 1.022e-56 Identity = 91/129 (70.54%), Postives = 104/129 (80.62%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIK-ILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
M L YYS LSL FI+T+ ILLQ QSRRL+NL NLHHLK P+HRTF LS KYGDI+SLWFGSRLV+V+SS SLV+ECF KND+VLANRPRFLTGKYIFY+Y+T+GSVSYG+HWRNLR
Sbjct: 1 MESLVYYSLLSLLFILTLTFILLQNQSRRLRNLPPGPPTIPVIGNLHHLKPPIHRTFTALSHKYGDIMSLWFGSRLVIVVSSPSLVQECFNKNDLVLANRPRFLTGKYIFYNYSTVGSVSYGEHWRNLR 129 HSP 2 Score: 51.9878 bits (123), Expect = 1.022e-56 Identity = 25/31 (80.65%), Postives = 28/31 (90.32%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RITTIDVLSN+RL SFL +RTDEANRL+Q L
Sbjct: 130 RITTIDVLSNHRLNSFLAIRTDEANRLVQKL 160
BLAST of BU964318 vs. Lotus protein
Match: chr4.CM0026.1220.r2.a (- phase: 0 ) HSP 1 Score: 162.925 bits (411), Expect = 1.132e-45 Identity = 79/123 (64.23%), Postives = 91/123 (73.98%), Query Frame = 1
Query: 40 YYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
YYS + IK+ L SR+ KNL NLHHLK PLHRTF LS+KYGD+ SLWFG+RLVVV+SS + V+ECF KNDVVLANRPRFL+GKYIFY+YTTLGS SYG+HWRNLR
Sbjct: 8 YYSLFYVALFAIIKLFLG--SRKFKNLPPGPTSLPIIGNLHHLKRPLHRTFRALSEKYGDVFSLWFGNRLVVVVSSFADVQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 128 HSP 2 Score: 38.5058 bits (88), Expect = 1.132e-45 Identity = 18/31 (58.06%), Postives = 24/31 (77.42%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RIT++DVLSN+R+ SF +R DE RLI+ L
Sbjct: 129 RITSLDVLSNHRINSFSPIRRDETTRLIRKL 159 HSP 3 Score: 119.013 bits (297), Expect = 1.569e-34 Identity = 51/103 (49.51%), Postives = 73/103 (70.87%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PEVL K K ++T VG+++++DE D+PKL YL+N+I+E +S+DCTIGG+ VP+DTI+L N WA+HRDP W++A +FKPERF+K+G E
Sbjct: 323 PEVLEKIKAELDTHVGQDRLVDESDIPKLTYLKNVINETLRLYTPAPLLLPHSASDDCTIGGYKVPRDTIVLINAWALHRDPQLWTEATTFKPERFDKKGELE 425 HSP 4 Score: 45.0542 bits (105), Expect = 1.569e-34 Identity = 26/71 (36.62%), Postives = 37/71 (52.11%), Query Frame = 3
Query: 804 RRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
++G+ KL+ FG+GRRACPG LA R + + QCF+W KR + +K P KA+C
Sbjct: 420 KKGELEKLIPFGLGRRACPGELLAIRAISMTLALL-IQCFDW-KRVSDEEIDMGERDGFVLMKSIPVKAMC 488
BLAST of BU964318 vs. Lotus protein
Match: LjSGA_046308.1 (+ phase: 0 /partial) HSP 1 Score: 135.576 bits (340), Expect = 1.146e-45 Identity = 61/100 (61.00%), Postives = 76/100 (76.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
P+VL KAK ++TQ+GK++++ EQDL KLPYLQNIISE YSSEDCT+GG+N+PKDTI+LTN + IHRDP WSDA +FKPERF+KEG
Sbjct: 25 PQVLKKAKNELDTQIGKDQLVQEQDLSKLPYLQNIISETFRLHPPAPLLLPHYSSEDCTVGGYNIPKDTIVLTNAFLIHRDPKYWSDATNFKPERFDKEG 124 HSP 2 Score: 65.855 bits (159), Expect = 1.146e-45 Identity = 37/71 (52.11%), Postives = 45/71 (63.38%), Query Frame = 3
Query: 804 RRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
+ G+ KL+AFG+GRRACPG+SLAQRTVGL G QCFEW +RE L K + PF+ALC
Sbjct: 122 KEGEINKLIAFGLGRRACPGMSLAQRTVGLTAGLL-VQCFEW-ERETEEKLEMREDKGITMPMLVPFQALC 190
BLAST of BU964318 vs. Lotus protein
Match: LjSGA_080540.1 (- phase: 0 /partial) HSP 1 Score: 137.887 bits (346), Expect = 2.231e-40 Identity = 63/100 (63.00%), Postives = 76/100 (76.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
P+VL KAK ++TQ+GK++++ EQDL KLPYLQNIISE YSSEDCT+GG+N+PKDTI+LTN + IHRDP WSDA SFKPERFEKEG
Sbjct: 24 PQVLKKAKNELDTQIGKDQLVQEQDLSKLPYLQNIISETLRLHPPAPLLLPHYSSEDCTVGGYNIPKDTIVLTNAFLIHRDPKYWSDATSFKPERFEKEG 123 HSP 2 Score: 45.8246 bits (107), Expect = 2.231e-40 Identity = 20/29 (68.97%), Postives = 25/29 (86.21%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTV 887
++ G+ KL+AFG+GRRACPGLSL QRTV
Sbjct: 120 EKEGEINKLIAFGLGRRACPGLSLGQRTV 148
BLAST of BU964318 vs. Lotus protein
Match: chr2.CM0250.70.r2.m (- phase: 0 ) HSP 1 Score: 107.457 bits (267), Expect = 9.250e-35 Identity = 50/100 (50.00%), Postives = 67/100 (67.00%), Query Frame = 1
Query: 514 EVLNKAKXXIETQVGKN-KIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
EVL K K ++T +G++ +++DE D+ +LPYLQNI+ E SSEDCTIG +N+P++TI+L N WAI+ DP WSD FKPERFEKEG
Sbjct: 326 EVLKKEKHELDTYIGQDHRLVDEADISQLPYLQNIVYETLRLHPAAPLLLPHVSSEDCTIGEYNIPRNTIVLVNAWAINTDPKLWSDPTCFKPERFEKEG 425 HSP 2 Score: 57.3806 bits (137), Expect = 9.250e-35 Identity = 31/72 (43.06%), Postives = 41/72 (56.94%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
++ G+ KLM FG+GRRACPG +L QRTVGL QCFEW KR + + K K+ +A+C
Sbjct: 422 EKEGETNKLMTFGLGRRACPGTNLGQRTVGLTLALL-IQCFEW-KRTSEKEIDMTEAKGATVPKKVSLEAMC 491 HSP 3 Score: 132.88 bits (333), Expect = 1.637e-31 Identity = 71/126 (56.35%), Postives = 81/126 (64.29%), Query Frame = 1
Query: 31 PLFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
PL + L LTF T RRL NL NLH L+ P HRTF TLSQ YG I SL FGSRLVVV+SS+S +ECF +ND+VLANRP FL GKYI Y+ TT+ + SYGDHWRNLR
Sbjct: 9 PLISFFLLLLTFFRT---------RRLHNLPPGPPCLPIIGNLHQLQQPFHRTFHTLSQTYGQIFSLRFGSRLVVVVSSSSAAQECFTRNDIVLANRPSFLLGKYISYNNTTVLNSSYGDHWRNLR 125
BLAST of BU964318 vs. Lotus protein
Match: chr2.CM0250.30.r2.m (- phase: 0 ) HSP 1 Score: 108.997 bits (271), Expect = 4.861e-33 Identity = 46/100 (46.00%), Postives = 67/100 (67.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
P +L KA+ +E+Q+G ++++E ++ K+PYL+ IISE YSSEDCT+GG++VP++T+++ N WAIHRDP W D L F PERFE G
Sbjct: 320 PALLEKARNELESQIGNERVMEEAEVTKVPYLEKIISETLRLHPAVPMLLPHYSSEDCTVGGYHVPRNTMLMVNAWAIHRDPNLWVDPLQFVPERFENGG 419 HSP 2 Score: 50.0618 bits (118), Expect = 4.861e-33 Identity = 28/65 (43.08%), Postives = 39/65 (60.00%), Query Frame = 3
Query: 825 LMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRR-NLI*WRVKVLPCLKRSPFKALC 1016
++ FG+GRR+CPG LAQRT+GL G QCFEW + ++ R ++P K P +ALC
Sbjct: 425 VIPFGMGRRSCPGSGLAQRTMGLTLGSL-IQCFEWKRIGTEEIDMTEGRGTIIP--KAIPLEALC 486 HSP 3 Score: 116.316 bits (290), Expect = 8.869e-32 Identity = 64/128 (50.00%), Postives = 73/128 (57.03%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
M Y F+IT K LL RR KNL NLH LK P+H F LSQKYG I S FGS VVV+SSAS EECF KND++ ANR K++ YD TT+ + SYGDHWRNLR
Sbjct: 1 MEATLTYILCFFIFLITAKFLLSR--RRFKNLPPGPRPLPLIGNLHQLKPPVHHFFHALSQKYGPIFSFRFGSLPVVVVSSASAAEECFTKNDIIFANRVHSSKTKHLAYDSTTVITASYGDHWRNLR 126 HSP 4 Score: 38.5058 bits (88), Expect = 8.869e-32 Identity = 18/31 (58.06%), Postives = 25/31 (80.65%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RI++I++LS +RL SFLGVR DE RL++ L
Sbjct: 127 RISSIEILSTHRLNSFLGVRKDETMRLLRKL 157
BLAST of BU964318 vs. Lotus protein
Match: chr1.CM0318.690.r2.d (+ phase: 0 ) HSP 1 Score: 81.2629 bits (199), Expect = 2.785e-22 Identity = 41/104 (39.42%), Postives = 58/104 (55.77%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWS--DALSFKPERFEKEGGS 816
P ++ +A+ I + VG ++I++E D+ LPYLQ I+ E SSE CTI G+ +P T + N+WAI RDP W + L F+PERF E GS
Sbjct: 328 PHMMERARQEINSVVGNSRIVEESDIVHLPYLQAIVKETLRIHPTGPLIVRE-SSESCTIWGYEIPAKTQLFVNIWAIGRDPNHWETHNPLEFRPERFISEEGS 430 HSP 2 Score: 41.5874 bits (96), Expect = 2.785e-22 Identity = 21/41 (51.22%), Postives = 24/41 (58.54%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
RG+ L+ FG GRR CPG SLA + V N QCFEW
Sbjct: 437 RGQHFHLIPFGSGRRGCPGTSLALQVVQTNLA-AMIQCFEW 476
BLAST of BU964318 vs. Lotus protein
Match: LjT48C16.90.r2.d (- phase: 0 ) HSP 1 Score: 75.8702 bits (185), Expect = 3.692e-22 Identity = 33/99 (33.33%), Postives = 55/99 (55.56%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKE 807
P+V+ +A+ I++ G +++ E DL LPY++ I+ E S+E C +GG+++P + N+W++ RDP W + L FKPERF E
Sbjct: 27 PQVMERARQEIDSVTGNKRLVQESDLLNLPYIRAIVKESLRIHPTLPLIGRR-STESCNVGGYDIPAKCPLFVNLWSMGRDPNVWENPLEFKPERFMSE 124 HSP 2 Score: 46.595 bits (109), Expect = 3.692e-22 Identity = 25/49 (51.02%), Postives = 26/49 (53.06%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRN 953
RG+ KLM FG GRR CPG SLA V N QCFEW RN
Sbjct: 132 RGQNFKLMPFGTGRRVCPGASLALLVVPTNLA-AMIQCFEWKVLHGERN 179
BLAST of BU964318 vs. Lotus protein
Match: LjSGA_078605.1 (+ phase: 2 /partial) HSP 1 Score: 87.4261 bits (215), Expect = 6.582e-21 Identity = 42/96 (43.75%), Postives = 54/96 (56.25%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERF 798
P VL K K + TQVGK K I E D+ KL YLQ + E SE+C +GG+ V K T ++TN+W I+ DP+ W D L F+PERF
Sbjct: 154 PHVLKKVKEELNTQVGKGKCISELDISKLVYLQATVKEALRLYPATPLSTPREFSENCNLGGYQVKKGTRLITNIWKINTDPSVWPDPLDFQPERF 249 HSP 2 Score: 30.8018 bits (68), Expect = 6.582e-21 Identity = 14/29 (48.28%), Postives = 18/29 (62.07%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTVGL 893
RG +L+ FG GRR CPG+S + V L
Sbjct: 259 RGHHFELLPFGCGRRICPGISFGLQMVQL 287
BLAST of BU964318 vs. Lotus protein
Match: chr4.CM0333.580.r2.m (+ phase: 0 ) HSP 1 Score: 87.4261 bits (215), Expect = 1.103e-20 Identity = 38/96 (39.58%), Postives = 57/96 (59.38%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERF 798
P +L + + ++T VG+ + + E DLP LPYLQ ++ E +SE C + G+++PK + +L NVWAI RDP W++ L FKPERF
Sbjct: 323 PRILAQVQQELDTVVGRERNVREDDLPHLPYLQAVVKETFRLHPSTPLSLPRVASESCEVLGYHIPKGSTLLVNVWAIARDPKEWAEPLEFKPERF 418 HSP 2 Score: 30.0314 bits (66), Expect = 1.103e-20 Identity = 14/29 (48.28%), Postives = 18/29 (62.07%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTVGL 893
+G K++ FG GRR C G+SL R V L
Sbjct: 429 KGNDFKVIPFGAGRRICAGMSLGLRMVQL 457 HSP 3 Score: 59.3066 bits (142), Expect = 2.300e-9 Identity = 38/128 (29.69%), Postives = 60/128 (46.88%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
M P F ++TF+I I +++ +R L N H+ H + A L++ +G ++ L G VVV +SA++ E+ +D ++RP KYI Y+Y L YG WR LR
Sbjct: 1 MFPWMIIGFATITFLIFIHRVVKFSTRPSLPLPPGPKPWPIIGNFPHMGPVPHHSLAALARAHGPLMHLKLGFVDVVVAASAAVAEQFLKVHDANFSSRPPNAGAKYIAYNYQDLVFAPYGARWRYLR 128
BLAST of BU964318 vs. Soybean peptides
Match: Glyma08g09460.1|PACid:16270538 () HSP 1 Score: 159.073 bits (401), Expect = 1.241e-43 Identity = 76/122 (62.30%), Postives = 92/122 (75.41%), Query Frame = 1
Query: 43 YSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
YS L + + +K+LLQ +R+ +NL NLHHLK PLHRTF LS KYG ++SLWFGSRLVVV+SS +L +ECF KNDVVLANRPRFL+GK+IFY+YTTLGS YG+HWRNLR
Sbjct: 9 YSLLYVFLFLLLKLLLQ--ARKFQNLPPGPPSLPIIGNLHHLKRPLHRTFRALSDKYGHVISLWFGSRLVVVVSSQTLFQECFTKNDVVLANRPRFLSGKHIFYNYTTLGSSPYGEHWRNLR 128 HSP 2 Score: 36.965 bits (84), Expect = 1.241e-43 Identity = 17/31 (54.84%), Postives = 23/31 (74.19%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RIT +DVLS +RL SF +R DE +RL++ L
Sbjct: 129 RITALDVLSTHRLHSFAAIRRDETHRLVRKL 159 HSP 3 Score: 117.087 bits (292), Expect = 5.105e-35 Identity = 55/100 (55.00%), Postives = 68/100 (68.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PEV +A+ +ET VG++ +++E DL KLPYL+NII E SSE+C IGGF VP DTI+L N W+IHRDP WS+A SFKPERFEKEG
Sbjct: 326 PEVFKRARDELETHVGQDHLLEESDLSKLPYLKNIIYETLRLYTPAPLLLPHSSSEECIIGGFKVPGDTIVLINAWSIHRDPKVWSEATSFKPERFEKEG 425 HSP 4 Score: 50.0618 bits (118), Expect = 5.105e-35 Identity = 29/72 (40.28%), Postives = 40/72 (55.56%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
++ G+ KL+AFG+GRRACPG LA R + L+ G QCFEW KR + + + P KA+C
Sbjct: 422 EKEGELDKLIAFGLGRRACPGEGLAMRALCLSLGLL-IQCFEW-KRVGDKEIDMREESGFTLSRLIPLKAMC 491
BLAST of BU964318 vs. Soybean peptides
Match: Glyma08g09450.1|PACid:16270537 () HSP 1 Score: 129.413 bits (324), Expect = 7.281e-42 Identity = 58/100 (58.00%), Postives = 71/100 (71.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PE+L KAK I+ VG+++++DE D+PKLPYLQNII E YSSE+CTIGGF +P+DTI+L N WAI RDP WSDA FKPERFE+EG
Sbjct: 300 PEILKKAKDEIDNMVGQDRLVDESDIPKLPYLQNIIYETLRLFAPAPLLLPHYSSEECTIGGFTIPRDTIVLINAWAIQRDPEHWSDATCFKPERFEQEG 399 HSP 2 Score: 52.7582 bits (125), Expect = 7.281e-42 Identity = 23/45 (51.11%), Postives = 32/45 (71.11%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++ G+ KL+ FG+GRRACPG+ LA R++GL G QCFEW +
Sbjct: 396 EQEGEANKLIPFGLGRRACPGIGLAHRSMGLTLGLL-IQCFEWKR 439 HSP 3 Score: 28.4906 bits (62), Expect = 7.281e-42 Identity = 11/24 (45.83%), Postives = 17/24 (70.83%), Query Frame = 2
Query: 932 KARXEEKLDMMEGKGIAMPXKIPI 1003
K +E++DM E KG+A+P IP+
Sbjct: 438 KRPTDEEIDMRENKGLALPKLIPL 461 HSP 4 Score: 145.206 bits (365), Expect = 3.762e-39 Identity = 66/104 (63.46%), Postives = 82/104 (78.85%), Query Frame = 1
Query: 97 QSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
Q RR+KNL NLH++K PLHR+ +LS+KYG I SLWFGSR VVVISS +L++ECF K+D+VLANRPRFLTGKY+FY+Y+++GS YGDHWRNLR
Sbjct: 3 QRRRVKNLPPCPPSLPLIGNLHYIKSPLHRSLLSLSEKYGPIFSLWFGSRFVVVISSPTLLQECFTKHDIVLANRPRFLTGKYLFYNYSSMGSSPYGDHWRNLR 106 HSP 5 Score: 35.8094 bits (81), Expect = 3.762e-39 Identity = 18/31 (58.06%), Postives = 22/31 (70.97%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RI TIDVLS +RL SF +R +E R+IQ L
Sbjct: 107 RIITIDVLSTSRLNSFFEIRREETMRVIQKL 137
BLAST of BU964318 vs. Soybean peptides
Match: Glyma09g05440.1|PACid:16274870 () HSP 1 Score: 147.132 bits (370), Expect = 2.049e-40 Identity = 73/132 (55.30%), Postives = 94/132 (71.21%), Query Frame = 1
Query: 13 SVKKMSPLFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
+V M P YS LSL F T+K L Q +SR+++NL NL+ ++ P+HR F +SQKYG+I+SLWFGSRLVVV+SS + +ECF K+DV LANR R L+GKYIFYD TT+GS S+G+HWRNLR
Sbjct: 2 TVITMPPFLSYSLLSLAFFFTLKYLFQ-RSRKVRNLPPGPTPLPIIGNLNLVEQPIHRFFHRMSQKYGNIISLWFGSRLVVVVSSPTAYQECFTKHDVTLANRVRSLSGKYIFYDNTTVGSCSHGEHWRNLR 132 HSP 2 Score: 38.1206 bits (87), Expect = 2.049e-40 Identity = 18/31 (58.06%), Postives = 23/31 (74.19%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RIT++DVLS R+ SF G+R+DE RLI L
Sbjct: 133 RITSLDVLSTQRVHSFSGIRSDETKRLIHRL 163 HSP 3 Score: 109.383 bits (272), Expect = 1.834e-31 Identity = 50/100 (50.00%), Postives = 67/100 (67.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PEVL KA+ ++ QVG +++++E DLPKLPYL+ I+ E +SED I GFNVP+DTI++ N WA+ RDP W DA SFKPERF++EG
Sbjct: 326 PEVLQKARDELDAQVGPDRLLNESDLPKLPYLRKIVLETLRLYPPAPILIPHVASEDINIEGFNVPRDTIVIINGWAMQRDPKIWKDATSFKPERFDEEG 425 HSP 4 Score: 45.8246 bits (107), Expect = 1.834e-31 Identity = 26/69 (37.68%), Postives = 38/69 (55.07%), Query Frame = 3
Query: 810 GK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
G+ KL+AFG+GRRACPG +A ++V G QCF+W KR + L + + P +A+C
Sbjct: 425 GEEKKLVAFGMGRRACPGEPMAMQSVSYTLG-LMIQCFDW-KRVSEKKLDMTENNWITLSRLIPLEAMC 491
BLAST of BU964318 vs. Soybean peptides
Match: Glyma16g26520.1|PACid:16302972 () HSP 1 Score: 147.517 bits (371), Expect = 6.380e-39 Identity = 75/128 (58.59%), Postives = 85/128 (66.41%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
M+P + FL FI+ + I Q+RR KNL NLH LK PLHRTF LSQKYG I SLWFGSR VVV+SS V+ECF KND+VLANRP FLTGKYI Y+ TT+ YGDHWRNLR
Sbjct: 1 MTPFY---FLLFAFILFLSINFLIQTRRFKNLPPGPFSFPIIGNLHQLKQPLHRTFHALSQKYGPIFSLWFGSRFVVVVSSPLAVQECFTKNDIVLANRPHFLTGKYIGYNNTTVAVSPYGDHWRNLR 125 HSP 2 Score: 32.7278 bits (73), Expect = 6.380e-39 Identity = 18/37 (48.65%), Postives = 24/37 (64.86%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL*SMSRS 518
RI ++VLS +R+ SFL R DE RL+Q L SR+
Sbjct: 126 RIMALEVLSTHRINSFLENRRDEIMRLVQKLARDSRN 162 HSP 3 Score: 119.783 bits (299), Expect = 8.978e-38 Identity = 55/105 (52.38%), Postives = 72/105 (68.57%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSEPN 825
PE+L KAK ++T +G+++++DE D+PKLPYLQ+I+ E SSEDCTIG +N+P++TI+L N WAIHRDP WSD FKPERFE E SE N
Sbjct: 319 PEILKKAKNELDTHIGQDRLVDEPDIPKLPYLQSIVYETLRLHPAAPMLVPHLSSEDCTIGEYNIPQNTILLVNAWAIHRDPKLWSDPTHFKPERFENE--SEAN 421 HSP 4 Score: 56.6102 bits (135), Expect = 8.978e-38 Identity = 30/65 (46.15%), Postives = 40/65 (61.54%), Query Frame = 3
Query: 822 KLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
KL+ FG+GRRACPG +LAQRT+ L QCFEW KR ++ + K L K+ P +A+C
Sbjct: 422 KLLPFGLGRRACPGANLAQRTLSLTLALL-IQCFEW-KRTTKKEIDMTEGKGLTVSKKYPLEAMC 484
BLAST of BU964318 vs. Soybean peptides
Match: Glyma11g05530.1|PACid:16282792 () HSP 1 Score: 113.62 bits (283), Expect = 7.449e-37 Identity = 51/97 (52.58%), Postives = 69/97 (71.13%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFE 801
PEVL KA+ ++TQVG++++I+E D+ KL YLQNIISE SSEDCT+G ++VP++T+++ N WAIHRDP W+D SFKPERFE
Sbjct: 320 PEVLEKARVELDTQVGQDRLIEEADVTKLQYLQNIISETLRLHPPLSMLLPHLSSEDCTVGSYDVPRNTMLMVNAWAIHRDPKIWADPTSFKPERFE 416 HSP 2 Score: 53.1434 bits (126), Expect = 7.449e-37 Identity = 23/38 (60.53%), Postives = 29/38 (76.32%), Query Frame = 3
Query: 822 KLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
KL++FG+GRRACPG +AQRT+GL G QCFEW +
Sbjct: 424 KLISFGLGRRACPGAGMAQRTLGLTLGSL-IQCFEWKR 460 HSP 3 Score: 26.9498 bits (58), Expect = 7.449e-37 Identity = 11/20 (55.00%), Postives = 14/20 (70.00%), Query Frame = 2
Query: 944 EEKLDMMEGKGIAMPXKIPI 1003
EEK+DM EG G +P IP+
Sbjct: 463 EEKVDMTEGGGTIVPKAIPL 482 HSP 4 Score: 107.071 bits (266), Expect = 1.032e-28 Identity = 61/120 (50.83%), Postives = 78/120 (65.00%), Query Frame = 1
Query: 58 LTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLK-HPLHRTFATLSQKYG--DIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
L F+I++K+L +RLKN NLH LK PLHR LSQKYG +I+SL FGS+ V+V+SSAS EECF KND++ ANR R KYI +++T + + SYGDHWRNLR
Sbjct: 12 LIFLISLKLLFFR--KRLKNPAPSPPSLPIIGNLHQLKKQPLHRALYDLSQKYGPNNILSLRFGSQPVLVVSSASAAEECFTKNDIIFANRFRSSLTKYIGFNHTIITASSYGDHWRNLR 129 HSP 5 Score: 38.891 bits (89), Expect = 1.032e-28 Identity = 17/31 (54.84%), Postives = 26/31 (83.87%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RI+++++LSN+RL SFLGVR DE +L++ L
Sbjct: 130 RISSLEILSNHRLNSFLGVRKDETMKLLRKL 160
BLAST of BU964318 vs. Soybean peptides
Match: Glyma09g05460.1|PACid:16274872 () HSP 1 Score: 132.109 bits (331), Expect = 2.790e-36 Identity = 68/125 (54.40%), Postives = 86/125 (68.80%), Query Frame = 1
Query: 34 LFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
L YS L L +T+K L Q SR+L+NL NL+ L+ P+HR F +S++YG+IVSLWFGSRL VVISS + +ECF K+DV LANR L+GKYIFY+ TT+GS S+G HWRNLR
Sbjct: 7 LLSYSLLFLVLFLTLKSLFQ--SRKLRNLPPGPPPLPIIGNLNLLEQPIHRFFQRMSKEYGNIVSLWFGSRLAVVISSPTAYQECFTKHDVALANRLPSLSGKYIFYNNTTVGSCSHGQHWRNLR 129 HSP 2 Score: 39.2762 bits (90), Expect = 2.790e-36 Identity = 18/31 (58.06%), Postives = 23/31 (74.19%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RIT +DVLS R+ SF G+R+DE RL+Q L
Sbjct: 130 RITALDVLSTQRVHSFSGIRSDETKRLVQRL 160 HSP 3 Score: 112.464 bits (280), Expect = 2.890e-32 Identity = 53/100 (53.00%), Postives = 68/100 (68.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PEVL KAK ++TQVG++++++E DLPKLPYL+ II E SSED TI GFNVP+DTI++ N W + RDP W+DA FKPERF+ EG
Sbjct: 324 PEVLKKAKEELDTQVGQDRLLNESDLPKLPYLRKIILETLRLYPPAPILIPHVSSEDITIEGFNVPRDTIVIINGWGMQRDPHLWNDATCFKPERFDVEG 423 HSP 4 Score: 45.4394 bits (106), Expect = 2.890e-32 Identity = 26/69 (37.68%), Postives = 37/69 (53.62%), Query Frame = 3
Query: 810 GK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
G+ KL+AFG+GRRACPG +A ++V G QCF+W KR L + + P +A+C
Sbjct: 423 GEEKKLVAFGMGRRACPGEPMAMQSVSFTLGLL-IQCFDW-KRVSEEKLDMTENNWITLSRLIPLEAMC 489
BLAST of BU964318 vs. Soybean peptides
Match: Glyma09g05390.1|PACid:16274865 () HSP 1 Score: 132.109 bits (331), Expect = 4.738e-36 Identity = 63/104 (60.58%), Postives = 78/104 (75.00%), Query Frame = 1
Query: 97 QSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
Q R+ KNL NL+ L++PLHR F +S+ +G+I SLWFGSRL VV+SS S +ECF KNDVVLANRPR L+GK+IFY+YTT+GS SYG+HWRNLR
Sbjct: 4 QRRKYKNLPPGPPPLPIIGNLNLLENPLHRFFQRMSKTHGNIFSLWFGSRLAVVVSSPSAFQECFTKNDVVLANRPRSLSGKHIFYNYTTVGSSSYGEHWRNLR 107 HSP 2 Score: 38.5058 bits (88), Expect = 4.738e-36 Identity = 18/31 (58.06%), Postives = 22/31 (70.97%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RI +DVLS R+ SF G+R DE RLI+IL
Sbjct: 108 RIIALDVLSTQRIHSFTGIRKDETERLIRIL 138 HSP 3 Score: 100.908 bits (250), Expect = 3.596e-29 Identity = 44/100 (44.00%), Postives = 65/100 (65.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
P+VL K + ++TQVG+ ++++E DLP LPYL+ II E S +D TI FN+P+DTI++ N+WA+ RDP W++ FKPERF++EG
Sbjct: 302 PKVLMKVRDELDTQVGQERLVNESDLPNLPYLRKIILETLRLYPHAPLAIPHVSLDDITIKEFNIPRDTIVMVNIWAMQRDPLLWNEPTCFKPERFDEEG 401 HSP 4 Score: 46.595 bits (109), Expect = 3.596e-29 Identity = 20/38 (52.63%), Postives = 28/38 (73.68%), Query Frame = 3
Query: 822 KLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
KL++FG+GRRACPG +LA + VGL G QC++W +
Sbjct: 405 KLVSFGMGRRACPGETLAMQNVGLTLGLL-IQCYDWKR 441
BLAST of BU964318 vs. Soybean peptides
Match: Glyma09g05450.1|PACid:16274871 () HSP 1 Score: 130.954 bits (328), Expect = 6.166e-36 Identity = 67/125 (53.60%), Postives = 86/125 (68.80%), Query Frame = 1
Query: 34 LFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
L YS L L +T+K L Q R+L+NL NL+ L+ P+HR F +S++YG+IVSLWFGSRL VVISS + +ECF K+DV LANR L+GKYIFY+ TT+GS S+G+HWRNLR
Sbjct: 7 LLSYSLLFLVLFLTLKSLFQR--RKLRNLPPGPPPLPIIGNLNLLEQPIHRFFQRMSKEYGNIVSLWFGSRLAVVISSPTAYQECFTKHDVALANRLPSLSGKYIFYNNTTVGSCSHGEHWRNLR 129 HSP 2 Score: 39.2762 bits (90), Expect = 6.166e-36 Identity = 18/31 (58.06%), Postives = 23/31 (74.19%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RIT +DVLS R+ SF G+R+DE RL+Q L
Sbjct: 130 RITALDVLSTQRVHSFSGIRSDETKRLVQRL 160 HSP 3 Score: 112.849 bits (281), Expect = 2.220e-32 Identity = 53/100 (53.00%), Postives = 68/100 (68.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PEVL KAK ++TQVG++++++E DLPKLPYL+ II E SSED TI GFNVP+DTI++ N W + RDP W+DA FKPERF+ EG
Sbjct: 324 PEVLKKAKDELDTQVGQDRLLNESDLPKLPYLRKIILETLRLYPPAPILIPHVSSEDITIEGFNVPRDTIVIINGWGMQRDPQLWNDATCFKPERFDVEG 423 HSP 4 Score: 45.4394 bits (106), Expect = 2.220e-32 Identity = 26/69 (37.68%), Postives = 37/69 (53.62%), Query Frame = 3
Query: 810 GK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
G+ KL+AFG+GRRACPG +A ++V G QCF+W KR L + + P +A+C
Sbjct: 423 GEEKKLVAFGMGRRACPGEPMAMQSVSFTLGLL-IQCFDW-KRVSEEKLDMTENNWITLSRLIPLEAMC 489
BLAST of BU964318 vs. Soybean peptides
Match: Glyma15g16780.1|PACid:16299021 () HSP 1 Score: 126.716 bits (317), Expect = 5.105e-35 Identity = 62/122 (50.82%), Postives = 85/122 (69.67%), Query Frame = 1
Query: 43 YSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
Y+ L L + +K + Q SR+L+N+ NL+ L+ P+HR F +S++YG++VSLWFGSRL VVISS + +ECF K+DV LANR L+GKYIFY+ TT+GS S+G+HWRNLR
Sbjct: 10 YAVLFLVLFLGVKFVFQ--SRKLRNIPPGPPPLPIIGNLNLLEQPIHRFFQRMSKQYGNVVSLWFGSRLAVVISSPTAYQECFTKHDVALANRLPSLSGKYIFYNNTTVGSCSHGEHWRNLR 129 HSP 2 Score: 40.4318 bits (93), Expect = 5.105e-35 Identity = 23/49 (46.94%), Postives = 29/49 (59.18%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL*SMSRSVEQSKGRNRDSS 554
RIT +DVLS R+ SF G+R+DE RL+Q L S E+ R SS
Sbjct: 130 RITALDVLSTQRVHSFSGIRSDETKRLMQRLVLAKNSNEEEFARVEISS 178 HSP 3 Score: 111.309 bits (277), Expect = 6.381e-32 Identity = 51/100 (51.00%), Postives = 68/100 (68.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PEVL KA+ ++TQVG++++++E DLPKLPYL+ II E SSED TI GFN+P+DTI++ N W + RDP W+DA FKPERF+ EG
Sbjct: 326 PEVLKKARDELDTQVGQDRLLNESDLPKLPYLRKIILETLRLYPPAPILIPHVSSEDITIEGFNIPRDTIVIINGWGMQRDPQLWNDATCFKPERFDVEG 425 HSP 4 Score: 45.4394 bits (106), Expect = 6.381e-32 Identity = 26/69 (37.68%), Postives = 37/69 (53.62%), Query Frame = 3
Query: 810 GK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
G+ KL+AFG+GRRACPG +A ++V G QCF+W KR L + + P +A+C
Sbjct: 425 GEEKKLVAFGMGRRACPGEPMAMQSVSFTLGLL-IQCFDW-KRVSEEKLDMTENNWITLSRLIPLEAMC 491
BLAST of BU964318 vs. Soybean peptides
Match: Glyma09g05400.1|PACid:16274866 () HSP 1 Score: 121.709 bits (304), Expect = 1.583e-33 Identity = 59/128 (46.09%), Postives = 85/128 (66.41%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
M L + + + ++ +++ ++L+NL NL+ L+ P+HR F +S++YG+IVSLWFGSRL VVISS + +ECF K+DV LANR L+GKYIFY+ TT+GS S+G+HWRNLR
Sbjct: 1 MKKLLLKIIILIFSVTSLHCIVRATIKKLRNLPPGPPPLPIIGNLNLLEQPIHRFFQRMSKEYGNIVSLWFGSRLAVVISSPTAYQECFTKHDVALANRLPSLSGKYIFYNNTTVGSCSHGEHWRNLR 128 HSP 2 Score: 40.4318 bits (93), Expect = 1.583e-33 Identity = 20/39 (51.28%), Postives = 26/39 (66.67%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL*SMSRSVE 524
RIT++DVLS R+ SF G+R+DE RL+Q L S E
Sbjct: 129 RITSLDVLSTQRVHSFSGIRSDETKRLVQRLLQAKNSKE 167 HSP 3 Score: 112.464 bits (280), Expect = 2.890e-32 Identity = 53/100 (53.00%), Postives = 68/100 (68.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PEVL KAK ++TQVG++++++E DLPKLPYL+ II E SSED TI GFNVP+DTI++ N W + RDP W+DA FKPERF+ EG
Sbjct: 324 PEVLKKAKEELDTQVGQDRLLNESDLPKLPYLRKIILETLRLYPPAPILIPHVSSEDITIEGFNVPRDTIVIINGWGMQRDPHLWNDATCFKPERFDVEG 423 HSP 4 Score: 45.4394 bits (106), Expect = 2.890e-32 Identity = 26/69 (37.68%), Postives = 37/69 (53.62%), Query Frame = 3
Query: 810 GK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
G+ KL+AFG+GRRACPG +A ++V G QCF+W KR L + + P +A+C
Sbjct: 423 GEEKKLVAFGMGRRACPGEPMAMQSVSFTLGLL-IQCFDW-KRVSEEKLDMTENNWITLSRLIPLEAMC 489
BLAST of BU964318 vs. SwissProt
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2) HSP 1 Score: 159.073 bits (401), Expect = 7.920e-44 Identity = 78/120 (65.00%), Postives = 91/120 (75.83%), Query Frame = 1
Query: 49 FLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
+L+L FI I I ++R+ KNL NLHHLK PLHRTF LS+KYG + SLWFGSRLVVV+SSAS ++CF KNDVVLANRPRFL+GKYIFY+YTTLGS SYG+HWRNLR
Sbjct: 13 YLALFFIFNIVI----RARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 128 HSP 2 Score: 40.4318 bits (93), Expect = 7.920e-44 Identity = 18/28 (64.29%), Postives = 22/28 (78.57%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLI 491
RIT +DVLSN+R+ SF G+R DE RLI
Sbjct: 129 RITALDVLSNHRINSFSGIRRDETQRLI 156 HSP 3 Score: 118.242 bits (295), Expect = 3.806e-33 Identity = 52/103 (50.49%), Postives = 72/103 (69.90%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PEVL K K ++T VG+++++DE DLPKL YL+N+I+E +S++C IGG+ VP+DTI+L N WAIHRDP W++A +FKPERFEK+G E
Sbjct: 322 PEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGELE 424 HSP 4 Score: 45.4394 bits (106), Expect = 3.806e-33 Identity = 20/43 (46.51%), Postives = 29/43 (67.44%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
+++G+ KL+AFG+GRRACPG LA R + + QCF+W
Sbjct: 418 EKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALL-IQCFDW 459
BLAST of BU964318 vs. SwissProt
Match: C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1) HSP 1 Score: 105.145 bits (261), Expect = 8.263e-32 Identity = 47/99 (47.47%), Postives = 66/99 (66.67%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKE 807
P+V++KA+ I+ +VG +++I+E DL +LPYL+NI+ E +SEDC IG +++P+ T +L N WAIHRDP W D SFKPERFEKE
Sbjct: 326 PDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKE 424 HSP 2 Score: 53.1434 bits (126), Expect = 8.263e-32 Identity = 25/38 (65.79%), Postives = 28/38 (73.68%), Query Frame = 3
Query: 822 KLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
KL+AFG+GRRACPG LAQR VGL G QCFEW +
Sbjct: 429 KLLAFGLGRRACPGSGLAQRIVGLALGSL-IQCFEWER 465 HSP 3 Score: 21.1718 bits (43), Expect = 8.263e-32 Identity = 8/18 (44.44%), Postives = 12/18 (66.67%), Query Frame = 2
Query: 950 KLDMMEGKGIAMPXKIPI 1003
++DM EG G +P IP+
Sbjct: 470 EVDMKEGVGNTVPKAIPL 487 HSP 4 Score: 89.7373 bits (221), Expect = 1.395e-21 Identity = 54/128 (42.19%), Postives = 73/128 (57.03%), Query Frame = 1
Query: 43 YSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKY-----GDIVSLWFGSRLVVVISSASLV-EECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
YS SL F+I L+ + + L +L LK P+HRT + S+ G ++SL GSRLV V+SS + EECF KNDVVLANRP+ + GK++ Y+ T + + YGDHWRNLR
Sbjct: 11 YSIFSLIFLIISFKFLKPKKQNLP--PSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHWRNLR 136 HSP 5 Score: 35.039 bits (79), Expect = 1.395e-21 Identity = 17/31 (54.84%), Postives = 21/31 (67.74%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
R+ TI++ S +RL FL VRTDE RLI L
Sbjct: 137 RLCTIEIFSTHRLNCFLYVRTDEVRRLISRL 167
BLAST of BU964318 vs. SwissProt
Match: C81F1_ARATH (Cytochrome P450 81F1 OS=Arabidopsis thaliana GN=CYP81F1 PE=2 SV=2) HSP 1 Score: 100.908 bits (250), Expect = 1.209e-27 Identity = 44/103 (42.72%), Postives = 65/103 (63.11%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
PEVL KA+ I+ ++GK+++IDE D+ LPYLQN++SE ++D IGG++VP+DTI++ N WAIHRDP W + F P+R+ GS+
Sbjct: 320 PEVLEKARSEIDEKIGKDRLIDESDIAVLPYLQNVVSETFRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFNPDRYNDGCGSD 422 HSP 2 Score: 44.2838 bits (103), Expect = 1.209e-27 Identity = 22/36 (61.11%), Postives = 22/36 (61.11%), Query Frame = 3
Query: 822 KLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
KLM FG GRR CPG L QR V L G QCFEW
Sbjct: 427 KLMPFGNGRRTCPGAGLGQRIVTLALGSL-IQCFEW 461 HSP 3 Score: 105.531 bits (262), Expect = 5.206e-22 Identity = 56/126 (44.44%), Postives = 79/126 (62.70%), Query Frame = 1
Query: 34 LFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFI-KNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
+ Y+ L L F++ L ++++R NL +LH +K P+HR S +YG I SL FGSR VVVI+S SL +E F +ND+VL++RP LT KY+ Y++TT+G+ YGDHWRNLR
Sbjct: 1 MLYFILLPLLFLVISYKFLYSKTQRF-NLPPGPPSRPFVGHLHLMKPPIHRLLQRYSNQYGPIFSLRFGSRRVVVITSPSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHTTVGTAPYGDHWRNLR 125
BLAST of BU964318 vs. SwissProt
Match: F3PH_ARATH (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana GN=CYP75B1 PE=1 SV=1) HSP 1 Score: 93.9745 bits (232), Expect = 8.239e-22 Identity = 43/104 (41.35%), Postives = 66/104 (63.46%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERF----EKEG 810
P+++ KA+ ++ VG+++ ++E D+ +LPYLQ +I E +SE C I G+++PK + +LTN+WAI RDP +WSD L+FKPERF EK G
Sbjct: 322 PDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPGGEKSG 425 HSP 2 Score: 31.5722 bits (70), Expect = 8.239e-22 Identity = 14/27 (51.85%), Postives = 18/27 (66.67%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTV 887
+G +L+ FG GRR C GLSL RT+
Sbjct: 429 KGSDFELIPFGAGRRICAGLSLGLRTI 455 HSP 3 Score: 55.8398 bits (133), Expect = 4.730e-7 Identity = 39/129 (30.23%), Postives = 58/129 (44.96%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIKILLQTQSRRLKN-LXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
M+ LF L+ + ++I ++R N L NL H+ HRT + + YG I+ L G VVV +S S+ E+ +D A+RP K++ Y+Y L YG WR LR
Sbjct: 1 MATLFLTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMVTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRWRLLR 129
BLAST of BU964318 vs. SwissProt
Match: C93A3_SOYBN (Cytochrome P450 93A3 OS=Glycine max GN=CYP93A3 PE=2 SV=1) HSP 1 Score: 82.4185 bits (202), Expect = 1.072e-21 Identity = 40/103 (38.83%), Postives = 58/103 (56.31%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
P VL KA+ ++ VGK++I++E D+ LPYLQ I+ E SS + G+++P T + NVWAI RDP W + L F+PERF + G S+
Sbjct: 327 PGVLEKARQEMDAVVGKSRIVEESDIANLPYLQGIVRETLRLHPAGPLLFRE-SSRRAVVCGYDIPAKTRLFVNVWAIGRDPNHWENPLEFRPERFVENGKSQ 428 HSP 2 Score: 42.743 bits (99), Expect = 1.072e-21 Identity = 21/41 (51.22%), Postives = 26/41 (63.41%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
RG+ L+ FG GRRACPG SLA + V +N QCF+W
Sbjct: 432 RGQHYHLLPFGSGRRACPGTSLALQVVHVNLAVL-IQCFQW 471
BLAST of BU964318 vs. SwissProt
Match: C93A1_SOYBN (Cytochrome P450 93A1 OS=Glycine max GN=CYP93A1 PE=2 SV=1) HSP 1 Score: 81.6481 bits (200), Expect = 1.933e-20 Identity = 38/103 (36.89%), Postives = 59/103 (57.28%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
P+VL KA+ I+ VGK+++++E D+ LPYLQ I+ E SS+ + G+++P T + NVWAI RDP W F+PERF ++G ++
Sbjct: 326 PDVLEKARQEIDAVVGKSRMVEESDIANLPYLQAIVRETLRLHPGGPLVVRE-SSKSAVVCGYDIPAKTRLFVNVWAIGRDPNHWEKPFEFRPERFIRDGQNQ 427 HSP 2 Score: 39.2762 bits (90), Expect = 1.933e-20 Identity = 19/41 (46.34%), Postives = 24/41 (58.54%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
RG+ + FG GRR CPG SLA + V +N QCF+W
Sbjct: 431 RGQHYHFIPFGSGRRTCPGASLAWQVVPVNLAII-IQCFQW 470
BLAST of BU964318 vs. SwissProt
Match: C82A1_PEA (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum GN=CYP82A1 PE=2 SV=2) HSP 1 Score: 90.8929 bits (224), Expect = 5.522e-20 Identity = 42/96 (43.75%), Postives = 56/96 (58.33%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERF 798
P VL K K + T +GK + ++E D+ KL YL II E +EDCTIGG+++ K T ++ N+W IHRDP+ W D L FKPERF
Sbjct: 360 PHVLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIGGYHIKKGTRLMPNLWKIHRDPSVWPDPLEFKPERF 455 HSP 2 Score: 28.4906 bits (62), Expect = 5.522e-20 Identity = 13/27 (48.15%), Postives = 17/27 (62.96%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTV 887
RG+ +L+ FG GRR C G+SL V
Sbjct: 465 RGQNFELLPFGSGRRMCAGMSLGLHMV 491 HSP 3 Score: 63.929 bits (154), Expect = 1.737e-9 Identity = 28/76 (36.84%), Postives = 47/76 (61.84%), Query Frame = 1
Query: 181 HRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
H+T L KYG I ++ G+ +V+S+ L +ECF KND+V+++RP+ + + + Y+ +G YG +WR LR
Sbjct: 58 HKTLGALVDKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGWAPYGAYWRQLR 133
BLAST of BU964318 vs. SwissProt
Match: C76C1_ARATH (Cytochrome P450 76C1 OS=Arabidopsis thaliana GN=CYP76C1 PE=2 SV=1) HSP 1 Score: 80.8777 bits (198), Expect = 1.216e-19 Identity = 39/96 (40.62%), Postives = 55/96 (57.29%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERF 798
P+ + KA+ I+ +G+N I++E D+ KLPYLQ ++ E + D I GF V KDT +L NVWAI RDP+ W + F+PERF
Sbjct: 331 PKTMAKAQAEIDCVIGQNGIVEESDISKLPYLQAVVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSVWDNPSQFEPERF 426 HSP 2 Score: 37.3502 bits (85), Expect = 1.216e-19 Identity = 18/41 (43.90%), Postives = 24/41 (58.54%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
RG+ +L FG GRR CPG+ LA +TV L + F+W
Sbjct: 434 RGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYS-FDW 473
BLAST of BU964318 vs. SwissProt
Match: C93A2_SOYBN (Cytochrome P450 93A2 OS=Glycine max GN=CYP93A2 PE=2 SV=1) HSP 1 Score: 78.5666 bits (192), Expect = 1.582e-19 Identity = 40/103 (38.83%), Postives = 56/103 (54.37%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSE 819
P VL KA+ I+ VG ++II+E D+ LPYLQ I+ E SS+ + G+ +P T + NVWAI RDP W + F+PERF + G S+
Sbjct: 319 PCVLEKARQEIDAVVGNSRIIEESDIVNLPYLQAIVRETLRIHPGGPLIVRE-SSKSVVVCGYEIPAKTRLFVNVWAIGRDPNHWENPFEFRPERFFENGQSQ 420 HSP 2 Score: 39.2762 bits (90), Expect = 1.582e-19 Identity = 19/41 (46.34%), Postives = 25/41 (60.98%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
RG+ + FG GRR+CPG SLA + V +N QCF+W
Sbjct: 424 RGQHYHFIPFGSGRRSCPGTSLALQIVHVNLA-IMIQCFQW 463
BLAST of BU964318 vs. SwissProt
Match: C82A3_SOYBN (Cytochrome P450 82A3 OS=Glycine max GN=CYP82A3 PE=2 SV=1) HSP 1 Score: 89.3521 bits (220), Expect = 2.055e-19 Identity = 42/96 (43.75%), Postives = 58/96 (60.42%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERF 798
P L KAK I+ Q+GK++ I E D+ KL YLQ I+ E +E+C +GG+++ K T ++ N+W IHRDP+ WSD L FKPERF
Sbjct: 343 PLALGKAKEEIDMQIGKDEYIRESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHRDPSVWSDPLEFKPERF 438 HSP 2 Score: 28.1054 bits (61), Expect = 2.055e-19 Identity = 13/27 (48.15%), Postives = 16/27 (59.26%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTV 887
RG +L+ FG GRR C G+SL V
Sbjct: 448 RGHNFELLPFGSGRRVCAGMSLGLNMV 474
BLAST of BU964318 vs. Medicago proteins
Match: IMGA|Medtr5g016410.1 (Isoflavone 2'-hydroxylase (AHRD V1 **** P93147); contains Interpro domain(s) IPR002401 Cytochrome P450, E-class, group I chr05_pseudomolecule_IMGAG_V3.5 5626680-5629644 F EGN_Mt100125 20100825) HSP 1 Score: 149.828 bits (377), Expect = 5.176e-41 Identity = 76/126 (60.32%), Postives = 87/126 (69.05%), Query Frame = 1
Query: 37 FYYSFLSLTF--IITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
FY S + F IIT+K+ T SR+ KNL NLH LK PLH TF TLSQKYG I SLWFGSRLVVV+SS ++ +ECF KND+VLANRP FLTGKYI Y+ TT+ YGDHWRNLR
Sbjct: 4 FYLSLIISLFFLIITLKVFFNT-SRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLR 128 HSP 2 Score: 36.5798 bits (83), Expect = 5.176e-41 Identity = 18/31 (58.06%), Postives = 24/31 (77.42%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLIQIL 500
RI +I++LS++RL SFL +R DE RLIQ L
Sbjct: 129 RILSIEILSSHRLNSFLEIRRDEIMRLIQKL 159 HSP 3 Score: 112.079 bits (279), Expect = 1.559e-36 Identity = 48/100 (48.00%), Postives = 67/100 (67.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PE++ KAK ++T +G ++ +DE D+ KLPYLQ+I+ E SSED ++GG+N+P++TI++ N W IHRDP WSD FKPERFEKEG
Sbjct: 322 PEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKEG 421 HSP 4 Score: 59.3066 bits (142), Expect = 1.559e-36 Identity = 32/72 (44.44%), Postives = 44/72 (61.11%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI*WRVKVLPCLKRSPFKALC 1016
++ G+ KL++FG+GRRACPG +LAQRTVGL G QCFEW KR + K + K++ A+C
Sbjct: 418 EKEGEVNKLLSFGLGRRACPGENLAQRTVGLTLGLL-IQCFEW-KRIGEEKIDMVEAKGITVGKKTSLNAMC 487
BLAST of BU964318 vs. Medicago proteins
Match: IMGA|AC235671_19.1 (Cytochrome P450 monooxygenase (AHRD V1 ***- Q9XFX0_CICAR); contains Interpro domain(s) IPR002401 Cytochrome P450, E-class, group I AC235671.5 84821-82190 E EGN_Mt100125 20100825) HSP 1 Score: 150.984 bits (380), Expect = 4.297e-40 Identity = 75/129 (58.14%), Postives = 95/129 (73.64%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXX-NLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
++PL YYS LSL+FI+T+K+LLQ R+ KNL NLH++ PLHR+ LSQKYG I +LWFGS VVVISS +L+++C K+DV L+NRPRFLTGKY+FY+YT+L S YGDHWRNLR
Sbjct: 4 ITPLLYYSLLSLSFIVTLKLLLQR--RKFKNLPPGPSISLPIIGNLHNINLPLHRSLHNLSQKYGKIFTLWFGSSRVVVISSQTLLQQCLTKHDVDLSNRPRFLTGKYLFYNYTSLDSAPYGDHWRNLR 130 HSP 2 Score: 32.3426 bits (72), Expect = 4.297e-40 Identity = 15/28 (53.57%), Postives = 19/28 (67.86%), Query Frame = 3
Query: 408 RITTIDVLSNNRLTSFLGVRTDEANRLI 491
RI T+D+LS RL SF+G R E +LI
Sbjct: 131 RIITLDILSTQRLNSFVGSRRSETLKLI 158 HSP 3 Score: 113.235 bits (282), Expect = 8.208e-35 Identity = 50/100 (50.00%), Postives = 65/100 (65.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
P +L KAK + TQ+G +++++E D+P L Y+QNII E +SS +C I GF +P+DTI+L N WAI RDP WSDA FKPERFEKEG
Sbjct: 313 PNILKKAKDELNTQIGYDRLVEESDIPNLSYIQNIIYETLRLYSPAPLLLPRFSSNECNIEGFTIPRDTIVLINAWAIQRDPETWSDASCFKPERFEKEG 412 HSP 4 Score: 52.373 bits (124), Expect = 8.208e-35 Identity = 23/45 (51.11%), Postives = 31/45 (68.89%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++ G+ KL+AFG GRR CPG+ LA RT+ L+ G QCFEW +
Sbjct: 409 EKEGEANKLIAFGFGRRGCPGIGLAHRTMALSLGLL-IQCFEWKR 452
BLAST of BU964318 vs. Medicago proteins
Match: IMGA|Medtr4g035330.1 (Isoflavone 2'-hydroxylase (AHRD V1 ***- P93147); contains Interpro domain(s) IPR002401 Cytochrome P450, E-class, group I chr04_pseudomolecule_IMGAG_V3.5 11081348-11079840 E EGN_Mt100125 20100825) HSP 1 Score: 155.992 bits (393), Expect = 2.717e-38 Identity = 78/129 (60.47%), Postives = 96/129 (74.42%), Query Frame = 1
Query: 25 MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXX-NLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
MSPL YYS LSL+FI T+K+LLQ R+ KNL +LH++ PLHR+ LSQKYG+I SLWFGSR VVVISS +L+++C K+DVVL+NRP FLTGKY+FY+YT+L S YGDHWRNLR
Sbjct: 1 MSPLLYYSLLSLSFIFTLKLLLQR--RKFKNLPPSPSITLPLIGHLHYINLPLHRSLHNLSQKYGNIFSLWFGSRRVVVISSQALLQQCLAKHDVVLSNRPHFLTGKYLFYNYTSLDSAPYGDHWRNLR 127
BLAST of BU964318 vs. Medicago proteins
Match: IMGA|Medtr4g035320.1 (Cytochrome P450 (AHRD V1 ***- Q9ZWF2_GLYEC); contains Interpro domain(s) IPR002401 Cytochrome P450, E-class, group I chr04_pseudomolecule_IMGAG_V3.5 11077698-11076064 E EGN_Mt100125 20100825) HSP 1 Score: 107.071 bits (266), Expect = 2.134e-32 Identity = 46/100 (46.00%), Postives = 65/100 (65.00%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 810
PE+L KAK + T++G ++++DE D+ LPY+QNII E +SS++C I G+ +P+DTI+L N WAI RD W++A F PERFEKEG
Sbjct: 26 PEILKKAKDELYTKIGNDRLVDESDISNLPYIQNIIYETLRLYSPAPLLLPRFSSDECNIEGYTIPRDTIVLINAWAIQRDDETWNNAACFNPERFEKEG 125 HSP 2 Score: 50.447 bits (119), Expect = 2.134e-32 Identity = 23/45 (51.11%), Postives = 31/45 (68.89%), Query Frame = 3
Query: 801 KRRGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
++ G+ KL+AFG GRRACPG+ LA RT+ L+ QCFEW +
Sbjct: 122 EKEGEVNKLIAFGFGRRACPGIGLAYRTMWLSLALL-IQCFEWKR 165
BLAST of BU964318 vs. Medicago proteins
Match: IMGA|Medtr5g016440.1 (Cytochrome P450 81D1 (AHRD V1 ***- Q9FG65); contains Interpro domain(s) IPR002401 Cytochrome P450, E-class, group I chr05_pseudomolecule_IMGAG_V3.5 5635491-5637230 E EGN_Mt100125 20100825) HSP 1 Score: 101.293 bits (251), Expect = 4.571e-30 Identity = 44/97 (45.36%), Postives = 64/97 (65.98%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFE 801
PEV+ K K ++ +G+ +I+E + KL YLQNIISE S++ CT+GG++VP++T+++ N WAIHRDP W+D +SFKPERFE
Sbjct: 317 PEVVEKEKIELDNHIGQEHLIEEVEATKLKYLQNIISETLRLHPAAPMLLPHLSTKACTVGGYDVPQNTMLMVNAWAIHRDPNLWADPMSFKPERFE 413 HSP 2 Score: 43.8986 bits (102), Expect = 4.571e-30 Identity = 20/34 (58.82%), Postives = 23/34 (67.65%), Query Frame = 3
Query: 834 FGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXK 935
FG+GRRACPG LA RT+ L G QCFEW +
Sbjct: 426 FGMGRRACPGSGLALRTLSLTLGSL-IQCFEWKR 458 HSP 3 Score: 24.6386 bits (52), Expect = 4.571e-30 Identity = 9/20 (45.00%), Postives = 14/20 (70.00%), Query Frame = 2
Query: 944 EEKLDMMEGKGIAMPXKIPI 1003
+E +DM EG+G +P IP+
Sbjct: 461 KEGVDMTEGRGTLLPMAIPL 480 HSP 4 Score: 116.701 bits (291), Expect = 1.827e-26 Identity = 62/122 (50.82%), Postives = 76/122 (62.30%), Query Frame = 1
Query: 43 YSFLSLTFIITIKILLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
Y S +ITIK+L SRR K L NLH LK P+HR F LSQKYG I +L FGS+ V V+SSAS+ EECF KNDV+ ANR + KY+ ++ T + + SYGDHWRNLR
Sbjct: 8 YIIFSFILLITIKLLFF--SRRFKKLPPCPPSFPIIGNLHLLKQPIHRYFHDLSQKYGPIFTLKFGSQFVAVVSSASIAEECFTKNDVIFANRLTSIKTKYLGFNNTNIITSSYGDHWRNLR 127
BLAST of BU964318 vs. Medicago proteins
Match: IMGA|Medtr3g020780.1 (Cytochrome P450 (AHRD V1 ***- B9II24_POPTR); contains Interpro domain(s) IPR002401 Cytochrome P450, E-class, group I chr03_pseudomolecule_IMGAG_V3.5 5551177-5548768 E EGN_Mt100125 20100825) HSP 1 Score: 85.8853 bits (211), Expect = 6.651e-23 Identity = 39/103 (37.86%), Postives = 60/103 (58.25%), Query Frame = 1
Query: 514 EVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGSEP 822
+++ KA+ I++ VGK ++++E D+P LPY+Q+I+ E S+EDC IGG+ +P T + N+WAI RD W + L F+P RF E G P
Sbjct: 333 DMMEKAREEIDSIVGKKRLVEESDIPNLPYIQSIVKETLRLHPTGPLIVRQ-STEDCNIGGYYIPAKTTLFVNLWAIGRDSNYWENPLEFQPLRFINEVGQSP 434 HSP 2 Score: 39.6614 bits (91), Expect = 6.651e-23 Identity = 19/51 (37.25%), Postives = 28/51 (54.90%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEWXKREXRRNLI 959
+G+ +L++FG GRR+CPG SLA V QCF+W E ++
Sbjct: 438 KGQNVELLSFGAGRRSCPGSSLALHIVHTTLA-TMIQCFDWKVGEDGNGIV 487 HSP 3 Score: 59.3066 bits (142), Expect = 3.461e-9 Identity = 39/129 (30.23%), Postives = 60/129 (46.51%), Query Frame = 1
Query: 34 LFYYSFLSLTFIITIKI----LLQTQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
+F S L L I TIK LL R L L +L+ L H+ F +S +YG +V L FGS+ V++SS + ++C N+ NRP+ +YI Y + YG +W+ ++
Sbjct: 13 IFLASILLLRLIFTIKFHQKSLLPPSPRALPILG----------HLYLLTKLPHQAFHNISSRYGPLVYLLFGSKPCVLVSSPEMAKQCLKTNETCFLNRPKQSNLEYITYGSSDFALAPYGTYWKFMK 131
BLAST of BU964318 vs. Medicago proteins
Match: IMGA|Medtr7g092620.1 (Cytochrome P450 (AHRD V1 ***- B9II24_POPTR); contains Interpro domain(s) IPR002401 Cytochrome P450, E-class, group I chr07_pseudomolecule_IMGAG_V3.5 29448306-29450657 E EGN_Mt100125 20100825) HSP 1 Score: 81.6481 bits (200), Expect = 1.464e-22 Identity = 40/102 (39.22%), Postives = 56/102 (54.90%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEGGS 816
P ++ A+ I VG N+I++E D+ LPYLQ I+ E SSE CTI G+ +P T + N+W+I RDP W + L F+PERF E G+
Sbjct: 329 PHMMEIARQEINDVVGNNRIVEESDIINLPYLQAIVKETLRIHPTGPLIVRE-SSEKCTIQGYEIPAKTQLFVNIWSIGRDPNYWDNPLEFRPERFINEVGN 429 HSP 2 Score: 42.743 bits (99), Expect = 1.464e-22 Identity = 22/41 (53.66%), Postives = 24/41 (58.54%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTVGLNCGXCWFQCFEW 929
RG+ L+ FG GRRACPG SLA V N QCFEW
Sbjct: 433 RGQHFHLIPFGSGRRACPGTSLALHVVQTNLA-AMIQCFEW 472
BLAST of BU964318 vs. Medicago proteins
Match: IMGA|Medtr6g008620.1 (Cytochrome P450 (AHRD V1 *-*- B9MVI0_POPTR); contains Interpro domain(s) IPR002401 Cytochrome P450, E-class, group I chr06_pseudomolecule_IMGAG_V3.5 1829226-1833105 E EGN_Mt100125 20100825) HSP 1 Score: 92.4337 bits (228), Expect = 2.637e-21 Identity = 51/125 (40.80%), Postives = 69/125 (55.20%), Query Frame = 1
Query: 439 TVLPLFWEFGQTKQIDLYKYYKAC-----PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERF 798
T+L LF T + L + C P VL KAK ++T VGK ++++E D+ KL YLQ I+ E SE+CTIGG++V K T ++ N+W I DP+ WSD L FKPERF
Sbjct: 320 TILTLFIGGSDTSSVTLT--WAICLLLKNPLVLKKAKEELDTHVGKERLVNESDIGKLVYLQAIVKETLRLHPPGPLAAPREFSENCTIGGYHVRKGTRLMLNLWKIQTDPSVWSDPLEFKPERF 442 HSP 2 Score: 27.7202 bits (60), Expect = 2.637e-21 Identity = 12/27 (44.44%), Postives = 16/27 (59.26%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTV 887
RG +L+ FG GRR CP +S + V
Sbjct: 452 RGNHFELLPFGSGRRKCPAISFGLQIV 478 HSP 3 Score: 64.6994 bits (156), Expect = 8.237e-11 Identity = 26/80 (32.50%), Postives = 49/80 (61.25%), Query Frame = 1
Query: 169 KHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
K P HR L+ KYG I ++ GS+ +++++ + +ECF +D+V+++RP+ ++ +++ Y G YG +WR LR
Sbjct: 60 KQPPHRVLGPLADKYGPIFTIKLGSKHALILNNWEIAKECFTTHDMVVSSRPKLISTEHLAYGGAVFGFGPYGPYWRELR 139
BLAST of BU964318 vs. Medicago proteins
Match: IMGA|Medtr3g025230.1 (Flavonoid 3'-hydroxylase (AHRD V1 ***- Q8W3Y5_SOYBN); contains Interpro domain(s) IPR002401 Cytochrome P450, E-class, group I chr03_pseudomolecule_IMGAG_V3.5 7043977-7048958 E EGN_Mt100125 20100825) HSP 1 Score: 87.8113 bits (216), Expect = 4.462e-21 Identity = 40/96 (41.67%), Postives = 55/96 (57.29%), Query Frame = 1
Query: 511 PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERF 798
P +L K + ++ VG ++ + E D+P LPYLQ +I E +SE C I G+++PK + +L NVWAI RDP W D L FKPERF
Sbjct: 326 PRILAKVQEELDKVVGSDRNVKEDDIPNLPYLQAVIKETFRLHPSTPLSLPRIASESCEIFGYHIPKGSTLLVNVWAIARDPKEWVDPLEFKPERF 421 HSP 2 Score: 31.5722 bits (70), Expect = 4.462e-21 Identity = 14/29 (48.28%), Postives = 19/29 (65.52%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSLAQRTVGL 893
+G +++ FG GRR CPG+SL R V L
Sbjct: 433 KGNDFEVIPFGAGRRICPGMSLGLRMVQL 461 HSP 3 Score: 52.373 bits (124), Expect = 4.230e-7 Identity = 29/85 (34.12%), Postives = 45/85 (52.94%), Query Frame = 1
Query: 154 NLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
N+ HL H++ A L++ +G ++ L G V+V +S S+ E+ +D ++RP KYI Y+Y L YG WR LR
Sbjct: 44 NMPHLGPAPHQSIAALAKIHGPLMHLKLGFVDVIVAASGSVAEQFLKVHDANFSSRPPNTGAKYIAYNYQDLVFAPYGPRWRMLR 128
BLAST of BU964318 vs. Medicago proteins
Match: IMGA|Medtr6g008530.1 (Cytochrome P450 (AHRD V1 *-*- B9MVI0_POPTR); contains Interpro domain(s) IPR002401 Cytochrome P450, E-class, group I chr06_pseudomolecule_IMGAG_V3.5 1783402-1785896 E EGN_Mt100125 20100825) HSP 1 Score: 87.8113 bits (216), Expect = 1.658e-20 Identity = 48/125 (38.40%), Postives = 67/125 (53.60%), Query Frame = 1
Query: 439 TVLPLFWEFGQTKQIDLYKYYKAC-----PEVLNKAKXXIETQVGKNKIIDEQDLPKLPYLQNIISEXXXXXXXXXXXXXXYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERF 798
T+L LF T + L + C P V+ KAK ++ Q+GK + + E D+ KL YLQ I+ E SE+CT+GG++V K T ++TN+W I+ DP W D L FKPERF
Sbjct: 324 TILTLFAGGSDTTSVTLT--WALCLLLNNPLVMEKAKEELDAQIGKERCVCESDINKLTYLQAIVKETLRLYPPAPLSGPREFSENCTLGGYHVIKGTRLITNLWRINTDPNIWPDPLEFKPERF 446 HSP 2 Score: 29.6462 bits (65), Expect = 1.658e-20 Identity = 13/22 (59.09%), Postives = 15/22 (68.18%), Query Frame = 3
Query: 807 RGK*TKLMAFGIGRRACPGLSL 872
RG L+ FG GRR CPG+SL
Sbjct: 456 RGSNFVLLPFGSGRRICPGISL 477 HSP 3 Score: 63.929 bits (154), Expect = 1.405e-10 Identity = 25/76 (32.89%), Postives = 48/76 (63.16%), Query Frame = 1
Query: 181 HRTFATLSQKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLR 408
HR TL+ KYG + ++ GS+ +++++ + +ECF +D+V+++RP+ + K++ Y+ G YG +WR LR
Sbjct: 64 HRVLGTLADKYGPLFTIKLGSKRALILNNWEMAKECFTTHDMVVSSRPKLVATKHLGYNGAMFGFGPYGPYWRQLR 139 The following BLAST results are available for this feature:
BLAST of BU964318 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl) Total hits: 10
BLAST of BU964318 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot) Total hits: 10
BLAST of BU964318 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide) Total hits: 10
BLAST of BU964318 vs. TAIR peptide
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs TAIR 10 peptide) Total hits: 10
BLAST of BU964318 vs. TrEMBL
Analysis Date: 2011-04-27 (Homology Analysis: Pisum sativum unigene v2 vs Trembl) Total hits: 10
BLAST of BU964318 vs. Lotus protein
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins) Total hits: 10
BLAST of BU964318 vs. Soybean peptides
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides) Total hits: 10
BLAST of BU964318 vs. SwissProt
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Swissprot) Total hits: 10
BLAST of BU964318 vs. Medicago proteins
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Medicago proteins) Total hits: 10
InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v2
Date Performed: 2011-04-27
Analysis Name: InterProScan analysis for Pisum sativum unigene v1 Date Performed: 2010-12-29
Properties
Sequences
The
following sequences are available for this feature:
EST sequence >BU964318 ID=BU964318; Name=BU964318; organism=Pisum sativum; type=EST; length=1018bpback to top Annotated Terms
The
following terms have been associated with
this EST:
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