CD858756

EST Overview


Name	CD858756
Unique Name	CD858756
Type	EST
Organism	Pisum sativum (pea)
Sequence length	365

Libraries


Library Name	Type
LIBEST_013997 CI	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
KEGG analysis for Pisum sativum unigene v1	2011-01-05
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of CD858756 vs. TrEMBL
Match: A4ZYU3_9FABA (Oleate desaturase OS=Caragana korshinskii var. intermedia GN=FAD2-2 PE=2 SV=1)

HSP 1 Score: 224.557 bits (571), Expect = 2.604e-57
Identity = 101/106 (95.28%), Postives = 103/106 (97.17%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            NGFLVLITFLQHTHPALPHYDSSEWDWL+GAL TVDR YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREAREC+Y EPD
Sbjct:  263 NGFLVLITFLQHTHPALPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECVYAEPD 368

[Close]

BLAST of CD858756 vs. TrEMBL
Match: B7FIN9_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 2.205e-56
Identity = 100/106 (94.34%), Postives = 103/106 (97.17%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            NGFLVLITFLQHTHPALPHYDSSEWDWL+GAL TVDR YGI NKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILG+YYRLDETPFY+ALWREARECIYVEPD
Sbjct:  285 NGFLVLITFLQHTHPALPHYDSSEWDWLRGALATVDRDYGIQNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGDYYRLDETPFYQALWREARECIYVEPD 390

[Close]

BLAST of CD858756 vs. TrEMBL
Match: D2CM01_DAVIN (Delta-12 fatty acid desaturase OS=Davidia involucrata PE=2 SV=1)

HSP 1 Score: 216.468 bits (550), Expect = 7.092e-55
Identity = 95/106 (89.62%), Postives = 102/106 (96.23%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            NGFLVLIT+LQHTHPALPHYDSSEWDWL+GAL T+DR YG+LNKVFHNITDTHV HHLFSTMPHYHAMEATKAIKPILGEYY+LD TPFYKA+WREAREC+YVEPD
Sbjct:  262 NGFLVLITYLQHTHPALPHYDSSEWDWLRGALATMDRDYGVLNKVFHNITDTHVTHHLFSTMPHYHAMEATKAIKPILGEYYQLDGTPFYKAMWREARECLYVEPD 367

[Close]

BLAST of CD858756 vs. TrEMBL
Match: Q19AK7_SOYBN (Microsomal oleate desaturase FAD2-3 OS=Glycine max GN=FAD2-3 PE=2 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 1.210e-54
Identity = 98/106 (92.45%), Postives = 100/106 (94.34%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            NGFLVLITFLQHTHPALPHY SSEWDWL+GAL TVDR YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYR D TPF KA+WREARECIYVEPD
Sbjct:  263 NGFLVLITFLQHTHPALPHYTSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRFDGTPFVKAMWREARECIYVEPD 368

[Close]

BLAST of CD858756 vs. TrEMBL
Match: C6TK45_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 1.210e-54
Identity = 98/106 (92.45%), Postives = 100/106 (94.34%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            NGFLVLITFLQHTHPALPHY SSEWDWL+GAL TVDR YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYR D TPF KA+WREARECIYVEPD
Sbjct:  263 NGFLVLITFLQHTHPALPHYTSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRFDGTPFVKAMWREARECIYVEPD 368

[Close]

BLAST of CD858756 vs. TrEMBL
Match: C6G3F3_POROL (Omega-6 fatty acid desaturase (Fragment) OS=Portulaca oleracea GN=FAD2 PE=2 SV=1)

HSP 1 Score: 214.927 bits (546), Expect = 2.063e-54
Identity = 94/106 (88.68%), Postives = 101/106 (95.28%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            NGFLVLITFLQHTHP+LPHYDSSEWDWL+GAL TVDR YG+LNKVFHNITDTHV HHLFSTMPHYHAMEATKAIKPILGEYY+ D TPFYKA+WREA+EC+YVEPD
Sbjct:  262 NGFLVLITFLQHTHPSLPHYDSSEWDWLRGALATVDRDYGVLNKVFHNITDTHVGHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPFYKAMWREAKECLYVEPD 367

[Close]

BLAST of CD858756 vs. TrEMBL
Match: Q9LLL7_SESIN (Microsomal oleic acid desaturase OS=Sesamum indicum GN=FAD2 PE=2 SV=1)

HSP 1 Score: 213.772 bits (543), Expect = 4.597e-54
Identity = 93/106 (87.74%), Postives = 101/106 (95.28%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            NGFLVLITFLQHTHP+LPHYDSSEWDWL+GAL TVDR YG+LNKVFHNITDTHV HHLFSTMPHYHAMEATKAIKPILG+YY+ D TPFYKA+WREA+EC+YVEPD
Sbjct:  263 NGFLVLITFLQHTHPSLPHYDSSEWDWLRGALATVDRDYGVLNKVFHNITDTHVTHHLFSTMPHYHAMEATKAIKPILGQYYQFDGTPFYKAMWREAKECLYVEPD 368

[Close]

BLAST of CD858756 vs. TrEMBL
Match: Q5FB99_SOYBN (Microsomal omega-6 fatty acid desaturase OS=Glycine max GN=GmFAD2-2a PE=4 SV=1)

HSP 1 Score: 213.386 bits (542), Expect = 6.003e-54
Identity = 97/106 (91.51%), Postives = 100/106 (94.34%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            NGFLVLITFLQHTHPALPHY SSEWDWL+GAL TVDR YGIL+KVFHNITDTHVAH LFSTMPHYHAMEATKAIKPILGEYYR DETPF KA+WREARECIYVEPD
Sbjct:  263 NGFLVLITFLQHTHPALPHYTSSEWDWLRGALATVDRDYGILDKVFHNITDTHVAHELFSTMPHYHAMEATKAIKPILGEYYRFDETPFVKAMWREARECIYVEPD 368

[Close]

BLAST of CD858756 vs. TrEMBL
Match: Q5FB98_SOYBN (Microsomal omega-6 fatty acid desaturase OS=Glycine max GN=GmFAD2-2b PE=4 SV=1)

HSP 1 Score: 213.001 bits (541), Expect = 7.841e-54
Identity = 97/106 (91.51%), Postives = 99/106 (93.40%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            NGF VLITFLQHT PALPHY SSEWDWL+GAL TVDR YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYR DETPF KA+WREARECIYVEPD
Sbjct:  217 NGFSVLITFLQHTQPALPHYTSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRFDETPFVKAMWREARECIYVEPD 322

[Close]

BLAST of CD858756 vs. TrEMBL
Match: Q6QUM6_CUCPE (Omega-6 fatty acid desaturase OS=Cucurbita pepo GN=FAD2 PE=2 SV=1)

HSP 1 Score: 211.46 bits (537), Expect = 2.281e-53
Identity = 95/106 (89.62%), Postives = 99/106 (93.40%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            NGFLVLIT+LQHTHPALPHYDSSEWDWL+GAL TVDR YGILNKVFHNITDTHVAHHL STMPHYHAMEATKAIKPILGEYY  D TPF KA+WREA+ECIYVEPD
Sbjct:  263 NGFLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLVSTMPHYHAMEATKAIKPILGEYYHFDGTPFVKAMWREAKECIYVEPD 368

[Close]

BLAST of CD858756 vs. SwissProt
Match: FD6E2_SOYBN (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max GN=FAD2-2 PE=2 SV=1)

HSP 1 Score: 218.394 bits (555), Expect = 9.113e-57
Identity = 99/106 (93.40%), Postives = 101/106 (95.28%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            NGFLVLITFLQHTHPALPHY SSEWDWL+GAL TVDR YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYR DETPF KA+WREARECIYVEPD
Sbjct:  263 NGFLVLITFLQHTHPALPHYTSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRFDETPFVKAMWREARECIYVEPD 368

[Close]

BLAST of CD858756 vs. SwissProt
Match: FD6E1_SOYBN (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 1 OS=Glycine max GN=FAD2-1 PE=2 SV=1)

HSP 1 Score: 210.305 bits (534), Expect = 2.482e-54
Identity = 94/106 (88.68%), Postives = 100/106 (94.34%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            NGFLV IT+LQHTH ALPHYDSSEWDWLKGAL T+DR YGILNKVFH+ITDTHVAHHLFSTMPHYHAMEAT AIKPILGEYY+ D+TPFYKALWREAREC+YVEPD
Sbjct:  267 NGFLVTITYLQHTHFALPHYDSSEWDWLKGALATMDRDYGILNKVFHHITDTHVAHHLFSTMPHYHAMEATNAIKPILGEYYQFDDTPFYKALWREARECLYVEPD 372

[Close]

BLAST of CD858756 vs. SwissProt
Match: FAD6E_BRAJU (Omega-6 fatty acid desaturase, endoplasmic reticulum OS=Brassica juncea PE=2 SV=1)

HSP 1 Score: 206.453 bits (524), Expect = 3.584e-53
Identity = 91/106 (85.85%), Postives = 99/106 (93.40%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            N FLVLIT+LQHTHP+LPHYDSSEWDWL+GAL TVDR YGILNKVFHNITDTHVAHHLFSTMPHYHAME TKAIKPILG+YY+ D TP+ KA+WREA+ECIYVEPD
Sbjct:  264 NAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLFSTMPHYHAMEVTKAIKPILGDYYQFDGTPWVKAMWREAKECIYVEPD 369

[Close]

BLAST of CD858756 vs. SwissProt
Match: FAD6E_ARATH (Omega-6 fatty acid desaturase, endoplasmic reticulum OS=Arabidopsis thaliana GN=FAD2 PE=2 SV=1)

HSP 1 Score: 202.216 bits (513), Expect = 6.758e-52
Identity = 90/106 (84.91%), Postives = 100/106 (94.34%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            N FLVLIT+LQHTHP+LPHYDSSEWDWL+GAL TVDR YGILNKVFHNITDTHVAHHLFSTMPHY+AMEATKAIKPILG+YY+ D TP+Y A++REA+ECIYVEPD
Sbjct:  263 NAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAKECIYVEPD 368

[Close]

BLAST of CD858756 vs. SwissProt
Match: FAD12_CREAL (Delta(12) fatty acid dehydrogenase OS=Crepis alpina PE=2 SV=2)

HSP 1 Score: 158.688 bits (400), Expect = 8.569e-39
Identity = 64/104 (61.54%), Postives = 85/104 (81.73%), Query Frame = 3

Query:   54 FLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            F  +IT+L HTH +LPHYDSSEW+WL+GAL+T+DR +G LN V H++T THV HHLFS +PHYHA EA  AI  +LG++Y++D TP  KA+WREA+ECI++EP+
Sbjct:  258 FFDIITYLHHTHLSLPHYDSSEWNWLRGALSTIDRDFGFLNSVLHDVTHTHVMHHLFSYIPHYHAKEARDAINTVLGDFYKIDRTPILKAMWREAKECIFIEPE 361

[Close]

BLAST of CD858756 vs. SwissProt
Match: FAD12_MORIS (Delta(12) fatty acid desaturase OS=Mortierella isabellina PE=2 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 7.271e-30
Identity = 62/105 (59.05%), Postives = 74/105 (70.48%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYG-ILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVE 359
            N +LVLITFLQHT P LPHY    W++ +GAL TVDR +G  L+ +FH I  THVAHHLFS MP YHA EAT  +K +LGEYY  D +P   A+WR  REC +VE
Sbjct:  286 NFWLVLITFLQHTDPKLPHYREGAWNFQRGALCTVDRSFGKFLDHMFHGIVHTHVAHHLFSQMPFYHAEEATYHLKKLLGEYYVYDPSPIVVAVWRSFRECRFVE 390

[Close]

BLAST of CD858756 vs. SwissProt
Match: FAD12_MORAP (Delta(12) fatty acid desaturase OS=Mortierella alpina PE=2 SV=2)

HSP 1 Score: 129.028 bits (323), Expect = 7.271e-30
Identity = 62/105 (59.05%), Postives = 74/105 (70.48%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYG-ILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVE 359
            N +LVLITFLQHT P LPHY    W++ +GAL TVDR +G  L+ +FH I  THVAHHLFS MP YHA EAT  +K +LGEYY  D +P   A+WR  REC +VE
Sbjct:  286 NFWLVLITFLQHTDPKLPHYREGAWNFQRGALCTVDRSFGKFLDHMFHGIVHTHVAHHLFSQMPFYHAEEATYHLKKLLGEYYVYDPSPIVVAVWRSFRECRFVE 390

[Close]

BLAST of CD858756 vs. SwissProt
Match: FAD3E_TOBAC (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Nicotiana tabacum GN=FAD3 PE=2 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 3.062e-20
Identity = 52/109 (47.71%), Postives = 67/109 (61.47%), Query Frame = 3

Query:   54 FLVLITFLQHT--HPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDE----TPFY--KALWREARECIYV 356
            +L  +T+L H      LP Y   EW +L+G LTTVDR YG+ N + H+I  THV HHLF  +PHYH  EATKA KP+LG+YYR  +     PF+  K L R  ++  YV
Sbjct:  249 WLDFVTYLHHHGYEKKLPWYRGKEWSYLRGGLTTVDRDYGLFNNIHHDI-GTHVIHHLFPQIPHYHLREATKAAKPVLGKYYREPKKSGPIPFHLVKDLTRSMKQDHYV 356

[Close]

BLAST of CD858756 vs. SwissProt
Match: FAD3E_SOYBN (Omega-3 fatty acid desaturase, endoplasmic reticulum OS=Glycine max GN=FAD3 PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 8.909e-20
Identity = 45/86 (52.33%), Postives = 58/86 (67.44%), Query Frame = 3

Query:   54 FLVLITFLQHT--HPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDE 305
            +L  +T+L H   H  LP Y   EW +L+G LTTVDR YG +  + H+I  THV HHLF  +PHYH +EAT+A KP+LG+YYR  E
Sbjct:  252 WLDFVTYLHHHGHHQKLPWYRGKEWSYLRGGLTTVDRDYGWIYNIHHDI-GTHVIHHLFPQIPHYHLVEATQAAKPVLGDYYREPE 336

[Close]

BLAST of CD858756 vs. SwissProt
Match: FAD3C_BRANA (Omega-3 fatty acid desaturase, chloroplastic (Fragment) OS=Brassica napus GN=FAD7 PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 8.909e-20
Identity = 43/83 (51.81%), Postives = 58/83 (69.88%), Query Frame = 3

Query:   54 FLVLITFLQHT--HPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYR 296
            +L  +T+L H      LP Y   EW +L+G LTT+DR YG++N + H+I  THV HHLF  +PHYH +EAT+A KP+LG+YYR
Sbjct:  273 WLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGLINNIHHDI-GTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYR 354

[Close]

BLAST of CD858756 vs. TAIR peptide
Match: AT3G12120.2 (| Symbols: FAD2 | fatty acid desaturase 2 | chr3:3860592-3861743 REVERSE LENGTH=383)

HSP 1 Score: 202.216 bits (513), Expect = 5.772e-53
Identity = 90/106 (84.91%), Postives = 100/106 (94.34%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            N FLVLIT+LQHTHP+LPHYDSSEWDWL+GAL TVDR YGILNKVFHNITDTHVAHHLFSTMPHY+AMEATKAIKPILG+YY+ D TP+Y A++REA+ECIYVEPD
Sbjct:  263 NAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAKECIYVEPD 368

[Close]

BLAST of CD858756 vs. TAIR peptide
Match: AT3G12120.1 (| Symbols: FAD2 | fatty acid desaturase 2 | chr3:3860592-3861743 REVERSE LENGTH=383)

HSP 1 Score: 202.216 bits (513), Expect = 5.772e-53
Identity = 90/106 (84.91%), Postives = 100/106 (94.34%), Query Frame = 3

Query:   48 NGFLVLITFLQHTHPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDETPFYKALWREARECIYVEPD 365
            N FLVLIT+LQHTHP+LPHYDSSEWDWL+GAL TVDR YGILNKVFHNITDTHVAHHLFSTMPHY+AMEATKAIKPILG+YY+ D TP+Y A++REA+ECIYVEPD
Sbjct:  263 NAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRDYGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAKECIYVEPD 368

[Close]

BLAST of CD858756 vs. TAIR peptide
Match: AT3G11170.1 (| Symbols: FAD7, FADD | fatty acid desaturase 7 | chr3:3499959-3502126 FORWARD LENGTH=446)

HSP 1 Score: 95.5153 bits (236), Expect = 7.609e-21
Identity = 43/83 (51.81%), Postives = 58/83 (69.88%), Query Frame = 3

Query:   54 FLVLITFLQHT--HPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYR 296
            +L  +T+L H      LP Y   EW +L+G LTT+DR YG++N + H+I  THV HHLF  +PHYH +EAT+A KP+LG+YYR
Sbjct:  315 WLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGLINNIHHDI-GTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYR 396

[Close]

BLAST of CD858756 vs. TAIR peptide
Match: AT5G05580.1 (| Symbols: FAD8 | fatty acid desaturase 8 | chr5:1664331-1666345 FORWARD LENGTH=435)

HSP 1 Score: 93.5893 bits (231), Expect = 2.891e-20
Identity = 43/83 (51.81%), Postives = 57/83 (68.67%), Query Frame = 3

Query:   54 FLVLITFLQHT--HPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYR 296
            +L  +T+L H      LP Y   EW +L+G LTT+DR YG +N + H+I  THV HHLF  +PHYH +EAT+A KP+LG+YYR
Sbjct:  308 WLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDI-GTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYR 389

[Close]

BLAST of CD858756 vs. TAIR peptide
Match: AT2G29980.1 (| Symbols: FAD3 | fatty acid desaturase 3 | chr2:12781787-12784946 REVERSE LENGTH=386)

HSP 1 Score: 92.8189 bits (229), Expect = 4.932e-20
Identity = 44/87 (50.57%), Postives = 57/87 (65.52%), Query Frame = 3

Query:   54 FLVLITFLQHT--HPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRLDET 308
            +L  +T+L H      LP Y   EW +L+G LTT+DR YGI N + H+I  THV HHLF  +PHYH ++ATKA K +LG YYR  +T
Sbjct:  253 WLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIFNNIHHDI-GTHVIHHLFPQIPHYHLVDATKAAKHVLGRYYREPKT 338

[Close]

BLAST of CD858756 vs. TAIR peptide
Match: AT5G05580.2 (| Symbols: FAD8 | fatty acid desaturase 8 | chr5:1664331-1666190 FORWARD LENGTH=382)

HSP 1 Score: 72.7886 bits (177), Expect = 5.282e-14
Identity = 34/70 (48.57%), Postives = 45/70 (64.29%), Query Frame = 3

Query:   54 FLVLITFLQHT--HPALPHYDSSEWDWLKGALTTVDRHYGILNKVFHNITDTHVAHHLFSTMPHYHAMEA 257
            +L  +T+L H      LP Y   EW +L+G LTT+DR YG +N + H+I  THV HHLF  +PHYH +EA
Sbjct:  308 WLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGWINNIHHDI-GTHVIHHLFPQIPHYHLVEA 376

The following BLAST results are available for this feature:

BLAST of CD858756 vs. TrEMBL
BLAST of CD858756 vs. SwissProt
BLAST of CD858756 vs. TAIR peptide

BLAST of CD858756 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
A4ZYU3_9FABA	2.604e-57	95.28	Oleate desaturase OS=Caragana korshinskii var. int...	[more]
B7FIN9_MEDTR	2.205e-56	94.34	Putative uncharacterized protein OS=Medicago trunc...	[more]
D2CM01_DAVIN	7.092e-55	89.62	Delta-12 fatty acid desaturase OS=Davidia involucr...	[more]
Q19AK7_SOYBN	1.210e-54	92.45	Microsomal oleate desaturase FAD2-3 OS=Glycine max...	[more]
C6TK45_SOYBN	1.210e-54	92.45	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6G3F3_POROL	2.063e-54	88.68	Omega-6 fatty acid desaturase (Fragment) OS=Portul...	[more]
Q9LLL7_SESIN	4.597e-54	87.74	Microsomal oleic acid desaturase OS=Sesamum indicu...	[more]
Q5FB99_SOYBN	6.003e-54	91.51	Microsomal omega-6 fatty acid desaturase OS=Glycin...	[more]
Q5FB98_SOYBN	7.841e-54	91.51	Microsomal omega-6 fatty acid desaturase OS=Glycin...	[more]
Q6QUM6_CUCPE	2.281e-53	89.62	Omega-6 fatty acid desaturase OS=Cucurbita pepo GN...	[more]

BLAST of CD858756 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
FD6E2_SOYBN	9.113e-57	93.40	Omega-6 fatty acid desaturase, endoplasmic reticul...	[more]
FD6E1_SOYBN	2.482e-54	88.68	Omega-6 fatty acid desaturase, endoplasmic reticul...	[more]
FAD6E_BRAJU	3.584e-53	85.85	Omega-6 fatty acid desaturase, endoplasmic reticul...	[more]
FAD6E_ARATH	6.758e-52	84.91	Omega-6 fatty acid desaturase, endoplasmic reticul...	[more]
FAD12_CREAL	8.569e-39	61.54	Delta(12) fatty acid dehydrogenase OS=Crepis alpin...	[more]
FAD12_MORIS	7.271e-30	59.05	Delta(12) fatty acid desaturase OS=Mortierella isa...	[more]
FAD12_MORAP	7.271e-30	59.05	Delta(12) fatty acid desaturase OS=Mortierella alp...	[more]
FAD3E_TOBAC	3.062e-20	47.71	Omega-3 fatty acid desaturase, endoplasmic reticul...	[more]
FAD3E_SOYBN	8.909e-20	52.33	Omega-3 fatty acid desaturase, endoplasmic reticul...	[more]
FAD3C_BRANA	8.909e-20	51.81	Omega-3 fatty acid desaturase, chloroplastic (Frag...	[more]

BLAST of CD858756 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 6


Match Name	E-value	Identity	Description
AT3G12120.2	5.772e-53	84.91	\| Symbols: FAD2 \| fatty acid desaturase 2 \| chr3:3...	[more]
AT3G12120.1	5.772e-53	84.91	\| Symbols: FAD2 \| fatty acid desaturase 2 \| chr3:3...	[more]
AT3G11170.1	7.609e-21	51.81	\| Symbols: FAD7, FADD \| fatty acid desaturase 7 \| ...	[more]
AT5G05580.1	2.891e-20	51.81	\| Symbols: FAD8 \| fatty acid desaturase 8 \| chr5:1...	[more]
AT2G29980.1	4.932e-20	50.57	\| Symbols: FAD3 \| fatty acid desaturase 3 \| chr2:1...	[more]
AT5G05580.2	5.282e-14	48.57	\| Symbols: FAD8 \| fatty acid desaturase 8 \| chr5:1...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005804	Fatty acid desaturase, type 1	PFAM	PF00487	FA_desaturase	coord: 5..97 score: 1.5
None	No IPR available	SEG	seg	seg	coord: 3..15 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD858756 ID=CD858756; Name=CD858756; organism=Pisum sativum; type=EST; length=365bp
GTCTTGCCTGTGTTGTGTGTGTTTACGGAGTTCCACTGCTCGTAGTCAAT
GGATTCTTGGTGTTGATCACATTTTTGCAGCATACTCACCCTGCGTTGCC
ACATTACGATTCCTCGGAGTGGGACTGGTTGAAAGGAGCTTTAACAACTG
TGGACAGGCATTACGGAATCCTGAACAAGGTCTTCCACAATATTACAGAC
ACTCATGTGGCACATCACTTGTTCTCCACAATGCCACATTATCATGCAAT
GGAGGCTACCAAGGCAATAAAACCAATTTTGGGAGAATATTATCGGTTGG
ACGAAACACCGTTTTACAAGGCATTATGGAGGGAAGCAAGAGAGTGTATC
TATGTTGAGCCAGAT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0006629	lipid metabolic process
GO:0055114	obsolete oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR005804	FA_desaturase_dom

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