AM162185

EST Overview


Name	AM162185
Unique Name	AM162185
Type	EST
Organism	Pisum sativum (pea)
Sequence length	485

Libraries


Library Name	Type
LIBEST_019360 Pisum sativum leaf vegetative	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v2	Pisum sativum unigene v2	EST
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Pisum sativum unigene v2	2011-03-01
Homology Analysis: Pisum sativum unigene v2 vs Trembl	2011-04-27
Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins	2011-04-21
Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides	2011-04-21
InterProScan analysis for Pisum sativum unigene v2	2011-04-27
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

[Close]

BLAST of AM162185 vs. TrEMBL
Match: C6T0E4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 7.236e-7
Identity = 33/73 (45.21%), Postives = 35/73 (47.95%), Query Frame = 1

Query:   67 MATETKAVTEDVKIXXXXXXXXXXXXXXXXXXXXXXXGKEIAQQWEXXXXXXXXXXXFSVQLRRELESTTEKN 285
            MATE KA TEDVKI                       GKE+ QQWE           FS+QLRRELES TEKN
Sbjct:    1 MATEPKAATEDVKIDLFEDDDEFEEFEINEEWDDKEEGKEVTQQWEDDWDDDDVSDDFSLQLRRELESNTEKN 73

[Close]

BLAST of AM162185 vs. TrEMBL
Match: C6T0E4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 9.048e-7
Identity = 33/73 (45.21%), Postives = 35/73 (47.95%), Query Frame = 1

Query:   67 MATETKAVTEDVKIXXXXXXXXXXXXXXXXXXXXXXXGKEIAQQWEXXXXXXXXXXXFSVQLRRELESTTEKN 285
            MATE KA TEDVKI                       GKE+ QQWE           FS+QLRRELES TEKN
Sbjct:    1 MATEPKAATEDVKIDLFEDDDEFEEFEINEEWDDKEEGKEVTQQWEDDWDDDDVSDDFSLQLRRELESNTEKN 73

[Close]

BLAST of AM162185 vs. Lotus protein
Match: chr1.CM0166.600.r2.a (- phase: 0 )

HSP 1 Score: 50.8322 bits (120), Expect = 2.555e-7
Identity = 29/73 (39.73%), Postives = 34/73 (46.58%), Query Frame = 1

Query:   67 MATETKAVTEDVKIXXXXXXXXXXXXXXXXXXXXXXXGKEIAQQWEXXXXXXXXXXXFSVQLRRELESTTEKN 285
            MATE KA TEDVKI                       G+++ QQWE           FS+QLRRELES  +KN
Sbjct:    1 MATEPKAATEDVKIDLFEDDDEFEEFEINEEWDDKEEGQDVTQQWEDDWDDDDVSDDFSLQLRRELESNIDKN 73

[Close]

BLAST of AM162185 vs. Lotus protein
Match: LjT18L24.110.r2.d (+ phase: 0 /est)

HSP 1 Score: 49.2914 bits (116), Expect = 7.435e-7
Identity = 32/48 (66.67%), Postives = 34/48 (70.83%), Query Frame = 3

Query:  156 RVG*QGGR*RNSSTVGG*LG***C**RFLSSAEKGAREHYREELNHLL 299
            R+G*QGGR R  S VG *LG***C*  F SSA+KG  E    ELNHLL
Sbjct:    1 RMG*QGGRERCDSAVGR*LG***C*R*FFSSAQKGIGEQQ**ELNHLL 48

[Close]

BLAST of AM162185 vs. Soybean peptides
Match: Glyma04g35230.3|PACid:16256828 ()

HSP 1 Score: 56.9954 bits (136), Expect = 8.888e-9
Identity = 33/73 (45.21%), Postives = 35/73 (47.95%), Query Frame = 1

Query:   67 MATETKAVTEDVKIXXXXXXXXXXXXXXXXXXXXXXXGKEIAQQWEXXXXXXXXXXXFSVQLRRELESTTEKN 285
            MATE KA TEDVKI                       GKE+ QQWE           FS+QLRRELES TEKN
Sbjct:    1 MATEPKAATEDVKIDLFEDDDEFEEFEINEEWDDKEEGKEVTQQWEDDWDDDDVSDDFSLQLRRELESNTEKN 73

[Close]

BLAST of AM162185 vs. Soybean peptides
Match: Glyma04g35230.2|PACid:16256827 ()

HSP 1 Score: 56.9954 bits (136), Expect = 8.888e-9
Identity = 33/73 (45.21%), Postives = 35/73 (47.95%), Query Frame = 1

Query:   67 MATETKAVTEDVKIXXXXXXXXXXXXXXXXXXXXXXXGKEIAQQWEXXXXXXXXXXXFSVQLRRELESTTEKN 285
            MATE KA TEDVKI                       GKE+ QQWE           FS+QLRRELES TEKN
Sbjct:    1 MATEPKAATEDVKIDLFEDDDEFEEFEINEEWDDKEEGKEVTQQWEDDWDDDDVSDDFSLQLRRELESNTEKN 73

[Close]

BLAST of AM162185 vs. Soybean peptides
Match: Glyma04g35230.1|PACid:16256826 ()

HSP 1 Score: 56.9954 bits (136), Expect = 8.888e-9
Identity = 33/73 (45.21%), Postives = 35/73 (47.95%), Query Frame = 1

Query:   67 MATETKAVTEDVKIXXXXXXXXXXXXXXXXXXXXXXXGKEIAQQWEXXXXXXXXXXXFSVQLRRELESTTEKN 285
            MATE KA TEDVKI                       GKE+ QQWE           FS+QLRRELES TEKN
Sbjct:    1 MATEPKAATEDVKIDLFEDDDEFEEFEINEEWDDKEEGKEVTQQWEDDWDDDDVSDDFSLQLRRELESNTEKN 73

[Close]

BLAST of AM162185 vs. Soybean peptides
Match: Glyma14g17380.3|PACid:16295632 ()

HSP 1 Score: 55.4546 bits (132), Expect = 2.586e-8
Identity = 32/73 (43.84%), Postives = 35/73 (47.95%), Query Frame = 1

Query:   67 MATETKAVTEDVKIXXXXXXXXXXXXXXXXXXXXXXXGKEIAQQWEXXXXXXXXXXXFSVQLRRELESTTEKN 285
            MATE KAVTEDVKI                       GKE+ QQWE           FS+QL+RELES T KN
Sbjct:    1 MATEPKAVTEDVKIDLFEDDDEFEEFEINEEWDDKEEGKEVTQQWEDDWDDDDVSDDFSLQLKRELESNTNKN 73

[Close]

BLAST of AM162185 vs. Soybean peptides
Match: Glyma14g17380.2|PACid:16295631 ()

HSP 1 Score: 55.4546 bits (132), Expect = 2.586e-8
Identity = 32/73 (43.84%), Postives = 35/73 (47.95%), Query Frame = 1

Query:   67 MATETKAVTEDVKIXXXXXXXXXXXXXXXXXXXXXXXGKEIAQQWEXXXXXXXXXXXFSVQLRRELESTTEKN 285
            MATE KAVTEDVKI                       GKE+ QQWE           FS+QL+RELES T KN
Sbjct:    1 MATEPKAVTEDVKIDLFEDDDEFEEFEINEEWDDKEEGKEVTQQWEDDWDDDDVSDDFSLQLKRELESNTNKN 73

[Close]

BLAST of AM162185 vs. Soybean peptides
Match: Glyma14g17380.1|PACid:16295630 ()

HSP 1 Score: 55.4546 bits (132), Expect = 2.586e-8
Identity = 32/73 (43.84%), Postives = 35/73 (47.95%), Query Frame = 1

Query:   67 MATETKAVTEDVKIXXXXXXXXXXXXXXXXXXXXXXXGKEIAQQWEXXXXXXXXXXXFSVQLRRELESTTEKN 285
            MATE KAVTEDVKI                       GKE+ QQWE           FS+QL+RELES T KN
Sbjct:    1 MATEPKAVTEDVKIDLFEDDDEFEEFEINEEWDDKEEGKEVTQQWEDDWDDDDVSDDFSLQLKRELESNTNKN 73

[Close]

BLAST of AM162185 vs. Soybean peptides
Match: Glyma06g19530.1|PACid:16263515 ()

HSP 1 Score: 53.9138 bits (128), Expect = 7.524e-8
Identity = 31/73 (42.47%), Postives = 34/73 (46.58%), Query Frame = 1

Query:   67 MATETKAVTEDVKIXXXXXXXXXXXXXXXXXXXXXXXGKEIAQQWEXXXXXXXXXXXFSVQLRRELESTTEKN 285
            MATE KA  +DVKI                       GKE+ QQWE           FS+QLRRELES TEKN
Sbjct:    1 MATEPKAAADDVKIDLFEDDDEFEEFEINEEWDDKEEGKEVTQQWEDDWDDDDVSDDFSLQLRRELESNTEKN 73

The following BLAST results are available for this feature:

BLAST of AM162185 vs. TrEMBL
BLAST of AM162185 vs. TrEMBL
BLAST of AM162185 vs. Lotus protein
BLAST of AM162185 vs. Soybean peptides

BLAST of AM162185 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 1


Match Name	E-value	Identity	Description
C6T0E4_SOYBN	7.236e-7	45.21	Putative uncharacterized protein OS=Glycine max PE...	[more]

BLAST of AM162185 vs. TrEMBL
Analysis Date: 2011-04-27 (Homology Analysis: Pisum sativum unigene v2 vs Trembl)
Total hits: 1


Match Name	E-value	Identity	Description
C6T0E4_SOYBN	9.048e-7	45.21	Putative uncharacterized protein OS=Glycine max PE...	[more]

BLAST of AM162185 vs. Lotus protein
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Lotus proteins)
Total hits: 2


Match Name	E-value	Identity	Description
chr1.CM0166.600.r2.a	2.555e-7	39.73	- phase: 0	[more]
LjT18L24.110.r2.d	7.435e-7	66.67	+ phase: 0 /est	[more]

BLAST of AM162185 vs. Soybean peptides
Analysis Date: 2011-04-21 (Homology Analysis: Pisum sativum unigene v2 vs Soybean peptides)
Total hits: 7


Match Name	E-value	Identity
Glyma04g35230.3\|PACid:16256828	8.888e-9	45.21	[more]
Glyma04g35230.2\|PACid:16256827	8.888e-9	45.21	[more]
Glyma04g35230.1\|PACid:16256826	8.888e-9	45.21	[more]
Glyma14g17380.3\|PACid:16295632	2.586e-8	43.84	[more]
Glyma14g17380.2\|PACid:16295631	2.586e-8	43.84	[more]
Glyma14g17380.1\|PACid:16295630	2.586e-8	43.84	[more]
Glyma06g19530.1\|PACid:16263515	7.524e-8	42.47	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v2
Date Performed: 2011-04-27


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007834	DSS1/SEM1	PFAM	PF05160	DSS1_SEM1	coord: 27..86 score: 4.8
None	No IPR available	SEG	seg	seg	coord: 2..12 score: NAcoord: 33..55 score: NAcoord: 65..75 score
None	No IPR available	SEG	seg	seg	coord: 25..48 score
None	No IPR available	SEG	seg	seg	coord: 6..16 score
None	No IPR available	SEG	seg	seg	coord: 3..33 score

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007834	DSS1/SEM1	PFAM	PF05160	DSS1_SEM1	coord: 27..86 score: 4.8
None	No IPR available	SEG	seg	seg	coord: 2..12 score: NAcoord: 33..55 score: NAcoord: 65..75 score
None	No IPR available	SEG	seg	seg	coord: 25..48 score
None	No IPR available	SEG	seg	seg	coord: 6..16 score
None	No IPR available	SEG	seg	seg	coord: 3..33 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v2
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>AM162185 ID=AM162185; Name=AM162185; organism=Pisum sativum; type=EST; length=485bp
GCGAACACTTAAAGGATCATCGTAGTTTTCATTCCCCCAATACCTGTTAC
AGATTCAGATTTACATATGGCGACCGAAACGAAGGCTGTTACCGAGGATG
TCAAGATCGATCTCTTCGAAGACGACGATGAGTTCGAAGAGTTTGAAATC
AATGAAGAGTGGGATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTG
GGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGC
TGAGAAGGGAGCTCGAGAGCACTACAGAGAAGAATTAAACCACCTGCTGG
CTATTATTGTTTGATATAACATGTGTAATGAATGAGTTGCAACTTAGGTA
TTTGAGGTGTTTACTTACCTACATAAGAACTTAAACTATGATGTGTTAGG
AAGGAAATGTGAGTAAGAAGAACAGAACACGAGGACATAATTCACATGTT
ATTTCTTTTAAACATCTTCATAGAAGTTATTGCGT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR007834	DSS1_SEM1

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