CD858639

EST Overview


Name	CD858639
Unique Name	CD858639
Type	EST
Organism	Pisum sativum (pea)
Sequence length	383

Libraries


Library Name	Type
LIBEST_013996 CE	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Pisum sativum unigene v1	Pisum sativum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Pisum sativum unigene v1	2010-12-27
Homology Analysis: Pisum sativum unigene v1 vs Trembl	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide	2011-02-03
InterProScan analysis for Pisum sativum unigene v1	2010-12-29

Homology

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BLAST of CD858639 vs. TrEMBL
Match: B9SSN2_RICCO (Nitrogen fixation protein nifU, putative OS=Ricinus communis GN=RCOM_1374380 PE=4 SV=1)

HSP 1 Score: 216.083 bits (549), Expect = 9.332e-55
Identity = 111/118 (94.07%), Postives = 116/118 (98.31%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLREKIPAIAAVQLL 372
            VRLKLQGACGSCPSSV TMK+GIERRLMEKIPEIVAVEPI+DEETGLELN+ENIEKVLEEIRPYLVGAAGGSLELVAIEEPI K+RITGPAAGV+TVRVAVTQKLREKIPAIAAVQLL
Sbjct:  109 VRLKLQGACGSCPSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEENIEKVLEEIRPYLVGAAGGSLELVAIEEPIAKIRITGPAAGVMTVRVAVTQKLREKIPAIAAVQLL 226

[Close]

BLAST of CD858639 vs. TrEMBL
Match: D7TGV2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_35.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00023274001 PE=4 SV=1)

HSP 1 Score: 215.312 bits (547), Expect = 1.592e-54
Identity = 111/118 (94.07%), Postives = 116/118 (98.31%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLREKIPAIAAVQLL 372
            VRLKLQGACGSCPSSV TMK+GIERRLMEKIPEIVAVEPI+DEETGLELN+ENIEKVLEEIRPYLVGAAGGSLELV I+EPIVKVRITGPAAGV+TVRVAVTQKLREKIPAIAAVQLL
Sbjct:  110 VRLKLQGACGSCPSSVMTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEENIEKVLEEIRPYLVGAAGGSLELVGIDEPIVKVRITGPAAGVMTVRVAVTQKLREKIPAIAAVQLL 227

HSP 2 Score: 56.6102 bits (135), Expect = 9.462e-7
Identity = 31/87 (35.63%), Postives = 53/87 (60.92%), Query Frame = 1

Query:  118 IVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPA----AGVITVRVAVTQKLREKIPAIAAVQ 366
            +V      D    L L  EN+E VL+EIRPYL+ + GG++ L  I+  +V++++ G      + V+T+++ + ++L EKIP I AV+
Sbjct:   62 VVKAVATPDSAVELPLTAENVESVLDEIRPYLI-SDGGNVALHEIDGNVVRLKLQGACGSCPSSVMTMKMGIERRLMEKIPEIVAVE 147

[Close]

BLAST of CD858639 vs. TrEMBL
Match: C6TBQ1_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 211.075 bits (536), Expect = 3.002e-53
Identity = 108/118 (91.53%), Postives = 116/118 (98.31%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLREKIPAIAAVQLL 372
            VRLKLQGACGSCPSSVTTMK+GIERRLMEKIPEIVAVEPI+DEETGLELN++NIEKVLEE+RPYLVGAA G+LELVAI+EPIVKVRITGPAA V+TVRVAVTQKLREKIPAIAAVQLL
Sbjct:  102 VRLKLQGACGSCPSSVTTMKMGIERRLMEKIPEIVAVEPIADEETGLELNEDNIEKVLEEMRPYLVGAADGTLELVAIDEPIVKVRITGPAASVLTVRVAVTQKLREKIPAIAAVQLL 219

[Close]

BLAST of CD858639 vs. TrEMBL
Match: B9N0C0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_827441 PE=4 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 1.141e-52
Identity = 109/118 (92.37%), Postives = 114/118 (96.61%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLREKIPAIAAVQLL 372
            VRLKLQGAC SC +SVTTMK+GIERRLMEKIPEIVAVE ISDEETGLEL +ENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVK+RITGPAAGV+TVRVAVTQKLREKIPAIAAVQLL
Sbjct:  117 VRLKLQGACSSCSASVTTMKMGIERRLMEKIPEIVAVEAISDEETGLELKEENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKIRITGPAAGVMTVRVAVTQKLREKIPAIAAVQLL 234

[Close]

BLAST of CD858639 vs. TrEMBL
Match: Q53ML8_ORYSJ (Similar to seven transmembrane protein Mlo4 OS=Oryza sativa subsp. japonica GN=LOC_Os11g07920 PE=4 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 2.151e-51
Identity = 104/118 (88.14%), Postives = 111/118 (94.07%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLREKIPAIAAVQLL 372
            VRLKLQGACGSCP+SVTTMK+GIERRLMEKIPEIVAVEPI+DEETGLELN ENIEKVL+EIRPYL G  GG LE VAIEEPIVKVR+TGPAAGV+TVRVA+TQKLREKIP IAAVQLL
Sbjct:  603 VRLKLQGACGSCPASVTTMKMGIERRLMEKIPEIVAVEPIADEETGLELNQENIEKVLDEIRPYLSGTGGGELEFVAIEEPIVKVRLTGPAAGVMTVRVALTQKLREKIPKIAAVQLL 720

[Close]

BLAST of CD858639 vs. TrEMBL
Match: Q0IU70_ORYSJ (Os11g0181500 protein OS=Oryza sativa subsp. japonica GN=Os11g0181500 PE=2 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 2.151e-51
Identity = 104/118 (88.14%), Postives = 111/118 (94.07%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLREKIPAIAAVQLL 372
            VRLKLQGACGSCP+SVTTMK+GIERRLMEKIPEIVAVEPI+DEETGLELN ENIEKVL+EIRPYL G  GG LE VAIEEPIVKVR+TGPAAGV+TVRVA+TQKLREKIP IAAVQLL
Sbjct:  110 VRLKLQGACGSCPASVTTMKMGIERRLMEKIPEIVAVEPIADEETGLELNQENIEKVLDEIRPYLSGTGGGELEFVAIEEPIVKVRLTGPAAGVMTVRVALTQKLREKIPKIAAVQLL 227

HSP 2 Score: 56.6102 bits (135), Expect = 9.462e-7
Identity = 37/121 (30.58%), Postives = 64/121 (52.89%), Query Frame = 1

Query:   22 RLKLQGACGSCP--SSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPA----AGVITVRVAVTQKLREKIPAIAAVQ 366
            ++ +   CG+    SS   +      R   +  ++V      D    L L  EN+E VL+E+RPYL+ A GG++ L  I+  +V++++ G      A V T+++ + ++L EKIP I AV+
Sbjct:   28 KVGIASPCGTAAHASSAPRLVAAATHRGRRRQQQVVKAIANPDPAVELPLTAENVEIVLDEVRPYLM-ADGGNVALHEIDGNVVRLKLQGACGSCPASVTTMKMGIERRLMEKIPEIVAVE 147

[Close]

BLAST of CD858639 vs. TrEMBL
Match: B8BJF8_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_35353 PE=4 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 2.151e-51
Identity = 104/118 (88.14%), Postives = 111/118 (94.07%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLREKIPAIAAVQLL 372
            VRLKLQGACGSCP+SVTTMK+GIERRLMEKIPEIVAVEPI+DEETGLELN ENIEKVL+EIRPYL G  GG LE VAIEEPIVKVR+TGPAAGV+TVRVA+TQKLREKIP IAAVQLL
Sbjct:  110 VRLKLQGACGSCPASVTTMKMGIERRLMEKIPEIVAVEPIADEETGLELNQENIEKVLDEIRPYLSGTGGGELEFVAIEEPIVKVRLTGPAAGVMTVRVALTQKLREKIPKIAAVQLL 227

HSP 2 Score: 56.6102 bits (135), Expect = 9.462e-7
Identity = 37/121 (30.58%), Postives = 64/121 (52.89%), Query Frame = 1

Query:   22 RLKLQGACGSCP--SSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPA----AGVITVRVAVTQKLREKIPAIAAVQ 366
            ++ +   CG+    SS   +      R   +  ++V      D    L L  EN+E VL+E+RPYL+ A GG++ L  I+  +V++++ G      A V T+++ + ++L EKIP I AV+
Sbjct:   28 KVGIASPCGTAAHASSAPRLVAAATHRGRRRQQQVVKAIANPDPAVELPLTAENVEIVLDEVRPYLM-ADGGNVALHEIDGNVVRLKLQGACGSCPASVTTMKMGIERRLMEKIPEIVAVE 147

[Close]

BLAST of CD858639 vs. TrEMBL
Match: C5Y664_SORBI (Putative uncharacterized protein Sb05g005270 OS=Sorghum bicolor GN=Sb05g005270 PE=4 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 3.670e-51
Identity = 103/118 (87.29%), Postives = 110/118 (93.22%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLREKIPAIAAVQLL 372
            VRLKLQGACGSCP+SVTTMK+GIERRLMEKIPEIVAVEPI+DEETGLELN ENIEKVL+EIRPYL G  GG LE V IEEPIVKVR+TGPAAGV+TVRVA+TQKLREKIP IAAVQLL
Sbjct:  107 VRLKLQGACGSCPASVTTMKMGIERRLMEKIPEIVAVEPIADEETGLELNQENIEKVLDEIRPYLAGTGGGELEFVTIEEPIVKVRLTGPAAGVMTVRVALTQKLREKIPKIAAVQLL 224

[Close]

BLAST of CD858639 vs. TrEMBL
Match: C4JAF3_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 3.670e-51
Identity = 103/118 (87.29%), Postives = 110/118 (93.22%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLREKIPAIAAVQLL 372
            VRLKLQGACGSCP+SVTTMK+GIERRLMEKIPEIVAVEPI+DEETGLELN ENIEKVL+EIRPYL G  GG LE V IEEPIVKVR+TGPAAGV+TVRVA+TQKLREKIP IAAVQLL
Sbjct:  108 VRLKLQGACGSCPASVTTMKMGIERRLMEKIPEIVAVEPIADEETGLELNQENIEKVLDEIRPYLAGTGGGELEFVTIEEPIVKVRLTGPAAGVMTVRVALTQKLREKIPKIAAVQLL 225

HSP 2 Score: 58.151 bits (139), Expect = 3.252e-7
Identity = 38/113 (33.63%), Postives = 65/113 (57.52%), Query Frame = 1

Query:   40 ACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPA----AGVITVRVAVTQKLREKIPAIAAVQ 366
            AC +     ++  LG  RRL + +  +   +P  +    L L  EN+E VL+E+RPYL+ A GG++ L  I+  +V++++ G      A V T+++ + ++L EKIP I AV+
Sbjct:   39 ACSAPRLVASSAPLGC-RRLRQVVQAVANPDPAIE----LPLTAENVEMVLDEVRPYLM-ADGGNVALHEIDGNVVRLKLQGACGSCPASVTTMKMGIERRLMEKIPEIVAVE 145

[Close]

BLAST of CD858639 vs. TrEMBL
Match: B6T8D2_MAIZE (NFU3 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 3.670e-51
Identity = 103/118 (87.29%), Postives = 110/118 (93.22%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLREKIPAIAAVQLL 372
            VRLKLQGACGSCP+SVTTMK+GIERRLMEKIPEIVAVEPI+DEETGLELN ENIEKVL+EIRPYL G  GG LE V IEEPIVKVR+TGPAAGV+TVRVA+TQKLREKIP IAAVQLL
Sbjct:  108 VRLKLQGACGSCPASVTTMKMGIERRLMEKIPEIVAVEPIADEETGLELNQENIEKVLDEIRPYLAGTGGGELEFVTIEEPIVKVRLTGPAAGVMTVRVALTQKLREKIPKIAAVQLL 225

HSP 2 Score: 59.3066 bits (142), Expect = 1.460e-7
Identity = 38/110 (34.55%), Postives = 63/110 (57.27%), Query Frame = 1

Query:   49 SCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPA----AGVITVRVAVTQKLREKIPAIAAVQ 366
            S PS V +      RRL + +  +   +P  +    L L  EN+E VL+E+RPYL+ A GG++ L  I+  +V++++ G      A V T+++ + ++L EKIP I AV+
Sbjct:   41 SAPSLVASSAPLGRRRLRQVVQAVANPDPAIE----LPLTAENVEMVLDEVRPYLM-ADGGNVALHEIDGNVVRLKLQGACGSCPASVTTMKMGIERRLMEKIPEIVAVE 145

[Close]

BLAST of CD858639 vs. SwissProt
Match: NIFU2_ARATH (NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana GN=NIFU2 PE=1 SV=1)

HSP 1 Score: 199.519 bits (506), Expect = 4.428e-51
Identity = 100/118 (84.75%), Postives = 111/118 (94.07%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLREKIPAIAAVQLL 372
            VR+KLQGACGSCPSS  TMK+GIERRLMEKIPEIVAVE + DEETGLELN+ENIEKVLEEIRPYL+G A GSL+LV IE+PIVK+RITGPAAGV+TVRVAVTQKLREKIP+IAAVQL+
Sbjct:  118 VRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEETGLELNEENIEKVLEEIRPYLIGTADGSLDLVEIEDPIVKIRITGPAAGVMTVRVAVTQKLREKIPSIAAVQLI 235

HSP 2 Score: 58.5362 bits (140), Expect = 1.220e-8
Identity = 39/124 (31.45%), Postives = 67/124 (54.03%), Query Frame = 1

Query:   25 LKLQGACGSCPSSVTTMKLGIER----RLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPA----AGVITVRVAVTQKLREKIPAIAAVQLL 372
            L  Q A G        ++ G+ R    R + +  ++V      D    + L +EN+E VL+EIRPYL+ + GG++ L  I+  IV+V++ G      +  +T+++ + ++L EKIP I AV+ L
Sbjct:   35 LSSQRALGLVARPCNPLRRGLSRFLSSRQLFRRSKVVKAVATPDPILEVPLTEENVESVLDEIRPYLM-SDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEAL 157

[Close]

BLAST of CD858639 vs. SwissProt
Match: NIFU1_ORYSJ (NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 5.243e-44
Identity = 86/118 (72.88%), Postives = 103/118 (87.29%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLREKIPAIAAVQLL 372
            VRLKLQGACGSCPSS+ T+K GIERRLMEKIP++ AVEP++D+ETGLELN+EN+EKVL EIRPYL G  GG L+ + I+ PIVKVR+TGPAA V TVR+AV++KLREKIP+I  VQLL
Sbjct:  108 VRLKLQGACGSCPSSLITIKRGIERRLMEKIPDVAAVEPVTDKETGLELNEENVEKVLNEIRPYLAGTGGGGLQFLMIKGPIVKVRLTGPAAVVRTVRIAVSKKLREKIPSIQIVQLL 225

[Close]

BLAST of CD858639 vs. SwissProt
Match: NIFU3_ARATH (NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana GN=NIFU3 PE=2 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 1.579e-40
Identity = 86/119 (72.27%), Postives = 99/119 (83.19%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETG-LELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLREKIPAIAAVQLL 372
            V LKLQGACGSCPSS  T+K+GIE RL +KIPEI++VE   + ETG LELNDENIEKVL E+RPYL G  GG LELV I+  +VKVR+TGPAAGV+TVRVA+TQKLRE IP+I AVQLL
Sbjct:  117 VVLKLQGACGSCPSSSMTLKMGIESRLRDKIPEIMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKVRLTGPAAGVMTVRVALTQKLRETIPSIGAVQLL 235

[Close]

BLAST of CD858639 vs. SwissProt
Match: NIFU1_ARATH (NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 2.626e-11
Identity = 42/114 (36.84%), Postives = 67/114 (58.77%), Query Frame = 1

Query:    1 SVVAAEVRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVA-VEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLRE 339
            SV    V LKLQGAC SCPSS TTM +GIER L EK  + +  +  + DEE   ++  E +   L+ +RP  +   GGS+E++++E     V+  GP +  + ++ A+ +K ++
Sbjct:  112 SVEDGVVSLKLQGACTSCPSSSTTMTMGIERVLKEKFGDALKDIRQVFDEEV-KQITVEAVNAHLDILRP-AIKNYGGSVEVLSVEGEDCVVKYVGPESIGMGIQAAIKEKFKD 223

[Close]

BLAST of CD858639 vs. SwissProt
Match: NFU1_DROSI (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila simulans GN=GD15490 PE=3 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 4.636e-8
Identity = 30/75 (40.00%), Postives = 47/75 (62.67%), Query Frame = 1

Query:   10 AAEVRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDE-ETGLELNDENIEKVLEEIRPYLVGAAGG 231
            A  V+LK+QG+C SCPSS+ T+K G++  L   IPE+ +VE + DE +  ++   E  EK L+ ++    G +GG
Sbjct:  208 AGVVKLKMQGSCSSCPSSIVTLKNGVQNMLQFYIPEVESVEQVFDEADRMIDSEFERFEKNLKTLKQQ--GPSGG 280

[Close]

BLAST of CD858639 vs. SwissProt
Match: NFU1_DROER (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila erecta GN=GG17526 PE=3 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 4.636e-8
Identity = 29/74 (39.19%), Postives = 45/74 (60.81%), Query Frame = 1

Query:   10 AAEVRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDE-ETGLELNDENIEKVLEEIRPYLVGAAG 228
            A  V+LK+QG+C SCPSS+ T+K G++  L   IPE+ +VE + DE +  ++   E  EK L+ ++      AG
Sbjct:  208 AGVVKLKMQGSCSSCPSSIVTLKNGVQNMLQFYIPEVESVEQVFDEADRMIDSEFERFEKNLKTLKQQEPSGAG 281

[Close]

BLAST of CD858639 vs. SwissProt
Match: YUTI_BACSU (Putative nitrogen fixation protein yutI OS=Bacillus subtilis GN=yutI PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 7.907e-8
Identity = 24/40 (60.00%), Postives = 32/40 (80.00%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPI 138
            V+L+L GACGSCPSS  T+K GIER L+E++P +V VE +
Sbjct:   71 VKLRLLGACGSCPSSTITLKAGIERALLEEVPGVVEVEQV 110

[Close]

BLAST of CD858639 vs. SwissProt
Match: NFU1_DROME (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila melanogaster GN=CG32500 PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 7.907e-8
Identity = 27/63 (42.86%), Postives = 42/63 (66.67%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDE-ETGLELNDENIEKVLEEIR 204
            V+LK+QG+C SCPSS+ T+K G++  L   IPE+ +VE + DE +  +E   E  EK L+ ++
Sbjct:  211 VKLKMQGSCSSCPSSIVTLKNGVQNMLQFYIPEVESVEQVFDEADRMIESEFERFEKNLKTLK 273

[Close]

BLAST of CD858639 vs. SwissProt
Match: NFU1_DROSE (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila sechellia GN=GM13534 PE=3 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.033e-7
Identity = 27/66 (40.91%), Postives = 43/66 (65.15%), Query Frame = 1

Query:   10 AAEVRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDE-ETGLELNDENIEKVLEEIR 204
            A  V+LK+QG+C SCPSS+ T+K G++  L   IPE+ +VE + DE +  ++   E  EK L+ ++
Sbjct:  208 AGVVKLKMQGSCSSCPSSIVTLKNGVQNMLQFYIPEVESVEQVFDEADRMIDSEFERFEKNLKTLK 273

[Close]

BLAST of CD858639 vs. SwissProt
Match: NFU1_DROYA (NFU1 iron-sulfur cluster scaffold homolog, mitochondrial OS=Drosophila yakuba GN=GE15286 PE=3 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.349e-7
Identity = 27/66 (40.91%), Postives = 43/66 (65.15%), Query Frame = 1

Query:   10 AAEVRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDE-ETGLELNDENIEKVLEEIR 204
            A  V+LK+QG+C SCPSS+ T+K G++  L   IPE+ +VE + DE +  ++   E  EK L+ ++
Sbjct:  208 AGVVKLKMQGSCSSCPSSIVTLKNGVQNMLQFYIPEVESVEQVFDEADRMVDSEFERFEKNLKTLK 273

[Close]

BLAST of CD858639 vs. TAIR peptide
Match: AT5G49940.1 (| Symbols: NFU2, ATCNFU2 | NIFU-like protein 2 | chr5:20315464-20317228 FORWARD LENGTH=235)

HSP 1 Score: 199.519 bits (506), Expect = 4.278e-52
Identity = 100/118 (84.75%), Postives = 111/118 (94.07%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLREKIPAIAAVQLL 372
            VR+KLQGACGSCPSS  TMK+GIERRLMEKIPEIVAVE + DEETGLELN+ENIEKVLEEIRPYL+G A GSL+LV IE+PIVK+RITGPAAGV+TVRVAVTQKLREKIP+IAAVQL+
Sbjct:  118 VRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEETGLELNEENIEKVLEEIRPYLIGTADGSLDLVEIEDPIVKIRITGPAAGVMTVRVAVTQKLREKIPSIAAVQLI 235

HSP 2 Score: 58.5362 bits (140), Expect = 1.179e-9
Identity = 39/124 (31.45%), Postives = 67/124 (54.03%), Query Frame = 1

Query:   25 LKLQGACGSCPSSVTTMKLGIER----RLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPA----AGVITVRVAVTQKLREKIPAIAAVQLL 372
            L  Q A G        ++ G+ R    R + +  ++V      D    + L +EN+E VL+EIRPYL+ + GG++ L  I+  IV+V++ G      +  +T+++ + ++L EKIP I AV+ L
Sbjct:   35 LSSQRALGLVARPCNPLRRGLSRFLSSRQLFRRSKVVKAVATPDPILEVPLTEENVESVLDEIRPYLM-SDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEAL 157

[Close]

BLAST of CD858639 vs. TAIR peptide
Match: AT4G25910.1 (| Symbols: NFU3, ATCNFU3 | NFU domain protein 3 | chr4:13164128-13165094 FORWARD LENGTH=236)

HSP 1 Score: 164.466 bits (415), Expect = 1.525e-41
Identity = 86/119 (72.27%), Postives = 99/119 (83.19%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETG-LELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLREKIPAIAAVQLL 372
            V LKLQGACGSCPSS  T+K+GIE RL +KIPEI++VE   + ETG LELNDENIEKVL E+RPYL G  GG LELV I+  +VKVR+TGPAAGV+TVRVA+TQKLRE IP+I AVQLL
Sbjct:  117 VVLKLQGACGSCPSSSMTLKMGIESRLRDKIPEIMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKVRLTGPAAGVMTVRVALTQKLRETIPSIGAVQLL 235

[Close]

BLAST of CD858639 vs. TAIR peptide
Match: AT5G49940.2 (| Symbols: NFU2, ATCNFU2 | NIFU-like protein 2 | chr5:20315464-20317067 FORWARD LENGTH=185)

HSP 1 Score: 101.293 bits (251), Expect = 1.586e-22
Identity = 49/57 (85.96%), Postives = 53/57 (92.98%), Query Frame = 1

Query:   19 VRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVAVEPISDEETGLELNDENIEKV 189
            VR+KLQGACGSCPSS  TMK+GIERRLMEKIPEIVAVE + DEETGLELN+ENIEKV
Sbjct:  118 VRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEALPDEETGLELNEENIEKV 174

HSP 2 Score: 58.5362 bits (140), Expect = 1.179e-9
Identity = 39/124 (31.45%), Postives = 67/124 (54.03%), Query Frame = 1

Query:   25 LKLQGACGSCPSSVTTMKLGIER----RLMEKIPEIVAVEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPA----AGVITVRVAVTQKLREKIPAIAAVQLL 372
            L  Q A G        ++ G+ R    R + +  ++V      D    + L +EN+E VL+EIRPYL+ + GG++ L  I+  IV+V++ G      +  +T+++ + ++L EKIP I AV+ L
Sbjct:   35 LSSQRALGLVARPCNPLRRGLSRFLSSRQLFRRSKVVKAVATPDPILEVPLTEENVESVLDEIRPYLM-SDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRLMEKIPEIVAVEAL 157

[Close]

BLAST of CD858639 vs. TAIR peptide
Match: AT4G01940.1 (| Symbols: NFU1, AtCNFU1 | NFU domain protein 1 | chr4:842265-843388 REVERSE LENGTH=231)

HSP 1 Score: 67.3958 bits (163), Expect = 2.537e-12
Identity = 42/114 (36.84%), Postives = 67/114 (58.77%), Query Frame = 1

Query:    1 SVVAAEVRLKLQGACGSCPSSVTTMKLGIERRLMEKIPEIVA-VEPISDEETGLELNDENIEKVLEEIRPYLVGAAGGSLELVAIEEPIVKVRITGPAAGVITVRVAVTQKLRE 339
            SV    V LKLQGAC SCPSS TTM +GIER L EK  + +  +  + DEE   ++  E +   L+ +RP  +   GGS+E++++E     V+  GP +  + ++ A+ +K ++
Sbjct:  112 SVEDGVVSLKLQGACTSCPSSSTTMTMGIERVLKEKFGDALKDIRQVFDEEV-KQITVEAVNAHLDILRP-AIKNYGGSVEVLSVEGEDCVVKYVGPESIGMGIQAAIKEKFKD 223

The following BLAST results are available for this feature:

BLAST of CD858639 vs. TrEMBL
BLAST of CD858639 vs. SwissProt
BLAST of CD858639 vs. TAIR peptide

BLAST of CD858639 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9SSN2_RICCO	9.332e-55	94.07	Nitrogen fixation protein nifU, putative OS=Ricinu...	[more]
D7TGV2_VITVI	1.592e-54	94.07	Whole genome shotgun sequence of line PN40024, sca...	[more]
C6TBQ1_SOYBN	3.002e-53	91.53	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9N0C0_POPTR	1.141e-52	92.37	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q53ML8_ORYSJ	2.151e-51	88.14	Similar to seven transmembrane protein Mlo4 OS=Ory...	[more]
Q0IU70_ORYSJ	2.151e-51	88.14	Os11g0181500 protein OS=Oryza sativa subsp. japoni...	[more]
B8BJF8_ORYSI	2.151e-51	88.14	Putative uncharacterized protein OS=Oryza sativa s...	[more]
C5Y664_SORBI	3.670e-51	87.29	Putative uncharacterized protein Sb05g005270 OS=So...	[more]
C4JAF3_MAIZE	3.670e-51	87.29	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
B6T8D2_MAIZE	3.670e-51	87.29	NFU3 OS=Zea mays PE=2 SV=1	[more]

BLAST of CD858639 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
NIFU2_ARATH	4.428e-51	84.75	NifU-like protein 2, chloroplastic OS=Arabidopsis ...	[more]
NIFU1_ORYSJ	5.243e-44	72.88	NifU-like protein 1, chloroplastic OS=Oryza sativa...	[more]
NIFU3_ARATH	1.579e-40	72.27	NifU-like protein 3, chloroplastic OS=Arabidopsis ...	[more]
NIFU1_ARATH	2.626e-11	36.84	NifU-like protein 1, chloroplastic OS=Arabidopsis ...	[more]
NFU1_DROSI	4.636e-8	40.00	NFU1 iron-sulfur cluster scaffold homolog, mitocho...	[more]
NFU1_DROER	4.636e-8	39.19	NFU1 iron-sulfur cluster scaffold homolog, mitocho...	[more]
YUTI_BACSU	7.907e-8	60.00	Putative nitrogen fixation protein yutI OS=Bacillu...	[more]
NFU1_DROME	7.907e-8	42.86	NFU1 iron-sulfur cluster scaffold homolog, mitocho...	[more]
NFU1_DROSE	1.033e-7	40.91	NFU1 iron-sulfur cluster scaffold homolog, mitocho...	[more]
NFU1_DROYA	1.349e-7	40.91	NFU1 iron-sulfur cluster scaffold homolog, mitocho...	[more]

BLAST of CD858639 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 4


Match Name	E-value	Identity	Description
AT5G49940.1	4.278e-52	84.75	\| Symbols: NFU2, ATCNFU2 \| NIFU-like protein 2 \| c...	[more]
AT4G25910.1	1.525e-41	72.27	\| Symbols: NFU3, ATCNFU3 \| NFU domain protein 3 \| ...	[more]
AT5G49940.2	1.586e-22	85.96	\| Symbols: NFU2, ATCNFU2 \| NIFU-like protein 2 \| c...	[more]
AT4G01940.1	2.537e-12	36.84	\| Symbols: NFU1, AtCNFU1 \| NFU domain protein 1 \| ...	[more]

InterPro

Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001075	NIF system FeS cluster assembly, NifU, C-terminal	PFAM	PF01106	NifU	coord: 4..45 score: 1.3e-12coord: 60..121 score: 7.1
None	No IPR available	PANTHER	PTHR11178	IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED	coord: 7..90 score: 2.3
None	No IPR available	SUPERFAMILY	117916	Fe-S cluster assembly (FSCA) domain-like	coord: 53..124 score: 6.4e-16coord: 7..52 score: 6.1
None	No IPR available	SEG	seg	seg	coord: 56..74 score

Properties


Property Name	Value
Singlet in analysis	Pisum sativum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CD858639 ID=CD858639; Name=CD858639; organism=Pisum sativum; type=EST; length=383bp
AGCGTGGTCGCGGCCGAGGTACGGTTAAAGTTACAAGGTGCTTGTGGATC
GTGTCCGAGTTCTGTTACGACAATGAAATTGGGCATTGAACGGCGATTAA
TGGAAAAGATCCCTGAAATAGTCGCAGTTGAACCAATATCTGACGAAGAG
ACTGGCCTTGAGCTAAATGATGAAAATATTGAGAAGGTATTAGAGGAAAT
AAGGCCATATCTGGTTGGGGCGGCTGGTGGATCTCTTGAATTGGTAGCCA
TTGAGGAGCCAATAGTAAAGGTTCGAATCACGGGTCCAGCTGCCGGTGTC
ATAACTGTGCGTGTTGCTGTTACACAAAAATTGCGAGAAAAAATACCTGC
CATCGCAGCAGTTCAGCTGTTATGATATTTTGG

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005506	iron ion binding
GO:0051536	iron-sulfur cluster binding

Vocabulary: **Biological Process**
Term	Definition
GO:0016226	iron-sulfur cluster assembly

Vocabulary: **INTERPRO**
Term	Definition
IPR001075	NIF_FeS_clus_asmbl_NifU_C

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