FG539000

EST Overview
NameFG539000
Unique NameFG539000
TypeEST
OrganismPisum sativum (pea)
Sequence length383
Libraries
Library NameType
LIBEST_023215 MeriZ Pisum sativum Shoot apical meristem ESTscdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Pisum sativum unigene v1Pisum sativum unigene v1EST
Homology
[Close]
BLAST of FG539000 vs. TrEMBL
Match: D7SLX0_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_21.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00018256001 PE=4 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.534e-15
Identity = 38/57 (66.67%), Postives = 47/57 (82.46%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKDNSYVVPYLKD 171
            T+LYTEDWIGL+ LDEAGKVKF++V G+HL ISR DMKTY+VPYL+D +   P + D
Sbjct:  253 TELYTEDWIGLKTLDEAGKVKFISVPGNHLAISRSDMKTYMVPYLEDQASTQPVIID 309
[Close]
BLAST of FG539000 vs. TrEMBL
Match: Q9LY31_ARATH (AT3G60340 protein OS=Arabidopsis thaliana GN=F27H5_130 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.610e-14
Identity = 39/49 (79.59%), Postives = 42/49 (85.71%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKDNS 147
            TKLYTEDWIGLR LDEAGKVKF+NVSG+HL IS  DMK +IVPYL D S
Sbjct:  245 TKLYTEDWIGLRTLDEAGKVKFVNVSGNHLQISHTDMKKHIVPYLCDKS 293
[Close]
BLAST of FG539000 vs. TrEMBL
Match: Q93Y57_ARATH (Putative palmitoyl-protein thioesterase OS=Arabidopsis thaliana GN=At3g60340 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.610e-14
Identity = 39/49 (79.59%), Postives = 42/49 (85.71%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKDNS 147
            TKLYTEDWIGLR LDEAGKVKF+NVSG+HL IS  DMK +IVPYL D S
Sbjct:  245 TKLYTEDWIGLRTLDEAGKVKFVNVSGNHLQISHTDMKKHIVPYLCDKS 293
[Close]
BLAST of FG539000 vs. TrEMBL
Match: Q8LAH5_ARATH (Palmitoyl-protein thioesterase-like OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.610e-14
Identity = 39/49 (79.59%), Postives = 42/49 (85.71%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKDNS 147
            TKLYTEDWIGLR LDEAGKVKF+NVSG+HL IS  DMK +IVPYL D S
Sbjct:  245 TKLYTEDWIGLRTLDEAGKVKFVNVSGNHLQISHTDMKKHIVPYLCDKS 293
[Close]
BLAST of FG539000 vs. TrEMBL
Match: A5APL1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_032028 PE=4 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.747e-14
Identity = 37/57 (64.91%), Postives = 46/57 (80.70%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKDNSYVVPYLKD 171
            T+LY EDWIGL+ LDEAGKVKF++V G+HL ISR DMKTY+VPYL+D +   P + D
Sbjct:  300 TELYXEDWIGLKTLDEAGKVKFISVPGNHLAISRSDMKTYMVPYLEDQASTQPVIID 356
[Close]
BLAST of FG539000 vs. TrEMBL
Match: B9GP59_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_755068 PE=4 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 6.119e-14
Identity = 36/49 (73.47%), Postives = 43/49 (87.76%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKDNS 147
            T LYTEDWIGL+ LDEAGKVKF+NVSG HLDIS+ DMK YI+PYL++ +
Sbjct:  251 TLLYTEDWIGLKTLDEAGKVKFINVSGGHLDISQTDMKKYILPYLEEQA 299
[Close]
BLAST of FG539000 vs. TrEMBL
Match: D7LRP2_ARALY (Palmitoyl protein thioesterase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_486507 PE=4 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 7.992e-14
Identity = 37/49 (75.51%), Postives = 42/49 (85.71%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKDNS 147
            TKLYTEDWIGL+ LDEAGKVKF+NVSG+HL IS  DMK +IVPYL D +
Sbjct:  245 TKLYTEDWIGLKTLDEAGKVKFVNVSGNHLQISHTDMKKHIVPYLCDKA 293
[Close]
BLAST of FG539000 vs. TrEMBL
Match: B9RD11_RICCO (Palmitoyl-protein thioesterase 1, putative OS=Ricinus communis GN=RCOM_1608620 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.044e-13
Identity = 45/77 (58.44%), Postives = 53/77 (68.83%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKDN-SYVVPYLKDQMQESFQS*KAIRSEKEYF 228
            TKLYTEDWIGL+ LDEAGKVKFL VSG+HL IS+  MK YIVPYL+D  SY      +Q+ E   S + I S   +F
Sbjct:  249 TKLYTEDWIGLKTLDEAGKVKFLTVSGNHLQISQDVMKKYIVPYLEDQASY------NQIMEGASSFRWISSIGNFF 319
[Close]
BLAST of FG539000 vs. TrEMBL
Match: Q6R749_TROMA (Putative palmitoyl-protein thioesterase OS=Tropaeolum majus PE=2 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.780e-13
Identity = 38/65 (58.46%), Postives = 49/65 (75.38%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKDNSYVV-PYLKDQMQESFQ 192
            T LYTEDWIGL+ LDEAG+VKF+NV+G+HLDIS+ DMK +IVPYL + +    P + +    SFQ
Sbjct:  249 TPLYTEDWIGLKTLDEAGRVKFVNVTGNHLDISKSDMKKHIVPYLVEQAPTASPIVTESKTISFQ 313
[Close]
BLAST of FG539000 vs. TrEMBL
Match: C5WRI6_SORBI (Putative uncharacterized protein Sb01g028940 OS=Sorghum bicolor GN=Sb01g028940 PE=4 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 5.180e-13
Identity = 36/45 (80.00%), Postives = 39/45 (86.67%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYL 135
            TKLYTEDWIGLR LDEAG+VKF++V G HL ISR DMK YIVPYL
Sbjct:  209 TKLYTEDWIGLRTLDEAGRVKFVSVPGGHLRISRSDMKKYIVPYL 253
[Close]
BLAST of FG539000 vs. SwissProt
Match: PPT1_RAT (Palmitoyl-protein thioesterase 1 OS=Rattus norvegicus GN=Ppt1 PE=1 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.762e-7
Identity = 24/46 (52.17%), Postives = 35/46 (76.09%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLK 138
            T LYTED +GL+++D+AGK+ FL   GDHL IS++    +I+P+LK
Sbjct:  261 TTLYTEDRLGLKKMDKAGKLVFLAKEGDHLQISKEWFTAHIIPFLK 306
[Close]
BLAST of FG539000 vs. SwissProt
Match: PPT1_MOUSE (Palmitoyl-protein thioesterase 1 OS=Mus musculus GN=Ppt1 PE=2 SV=2)

HSP 1 Score: 53.1434 bits (126), Expect = 5.125e-7
Identity = 23/44 (52.27%), Postives = 34/44 (77.27%), Query Frame = 1 
Query:    7 LYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLK 138
            LYTED +GL+++D+AGK+ FL   GDHL IS++    +I+P+LK
Sbjct:  263 LYTEDRLGLKKMDKAGKLVFLAKEGDHLQISKEWFTAHIIPFLK 306
[Close]
BLAST of FG539000 vs. TAIR peptide
Match: AT3G60340.2 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr3:22304395-22306389 FORWARD LENGTH=338)

HSP 1 Score: 82.4185 bits (202), Expect = 7.622e-17
Identity = 39/49 (79.59%), Postives = 42/49 (85.71%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKDNS 147
            TKLYTEDWIGLR LDEAGKVKF+NVSG+HL IS  DMK +IVPYL D S
Sbjct:  245 TKLYTEDWIGLRTLDEAGKVKFVNVSGNHLQISHTDMKKHIVPYLCDKS 293
[Close]
BLAST of FG539000 vs. TAIR peptide
Match: AT3G60340.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr3:22304395-22306389 FORWARD LENGTH=338)

HSP 1 Score: 82.4185 bits (202), Expect = 7.622e-17
Identity = 39/49 (79.59%), Postives = 42/49 (85.71%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKDNS 147
            TKLYTEDWIGLR LDEAGKVKF+NVSG+HL IS  DMK +IVPYL D S
Sbjct:  245 TKLYTEDWIGLRTLDEAGKVKFVNVSGNHLQISHTDMKKHIVPYLCDKS 293
[Close]
BLAST of FG539000 vs. TAIR peptide
Match: AT4G17470.3 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr4:9742922-9744468 REVERSE LENGTH=308)

HSP 1 Score: 71.633 bits (174), Expect = 1.345e-13
Identity = 29/49 (59.18%), Postives = 42/49 (85.71%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKDNS 147
            TKLYTEDWIGL+ LD+AGKV+F++V G H++I+ +D+  Y+VPYL++ S
Sbjct:  251 TKLYTEDWIGLKTLDDAGKVRFISVPGGHIEITEEDLVKYVVPYLQNES 299
[Close]
BLAST of FG539000 vs. TAIR peptide
Match: AT4G17470.2 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr4:9742922-9744468 REVERSE LENGTH=308)

HSP 1 Score: 71.633 bits (174), Expect = 1.345e-13
Identity = 29/49 (59.18%), Postives = 42/49 (85.71%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKDNS 147
            TKLYTEDWIGL+ LD+AGKV+F++V G H++I+ +D+  Y+VPYL++ S
Sbjct:  251 TKLYTEDWIGLKTLDDAGKVRFISVPGGHIEITEEDLVKYVVPYLQNES 299
[Close]
BLAST of FG539000 vs. TAIR peptide
Match: AT4G17470.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr4:9742922-9744468 REVERSE LENGTH=308)

HSP 1 Score: 71.633 bits (174), Expect = 1.345e-13
Identity = 29/49 (59.18%), Postives = 42/49 (85.71%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKDNS 147
            TKLYTEDWIGL+ LD+AGKV+F++V G H++I+ +D+  Y+VPYL++ S
Sbjct:  251 TKLYTEDWIGLKTLDDAGKVRFISVPGGHIEITEEDLVKYVVPYLQNES 299
[Close]
BLAST of FG539000 vs. TAIR peptide
Match: AT5G47330.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr5:19207170-19208711 FORWARD LENGTH=314)

HSP 1 Score: 67.781 bits (164), Expect = 1.943e-12
Identity = 28/47 (59.57%), Postives = 41/47 (87.23%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKD 141
            TKLYTEDWIGL+ LD+AGKVKF++V+G+H+ +  +D+  ++VPYL+D
Sbjct:  250 TKLYTEDWIGLKTLDDAGKVKFVSVAGEHIRMVDEDVVKHVVPYLQD 296
[Close]
BLAST of FG539000 vs. TAIR peptide
Match: AT5G47340.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr5:19209507-19211134 FORWARD LENGTH=317)

HSP 1 Score: 67.3958 bits (163), Expect = 2.537e-12
Identity = 28/47 (59.57%), Postives = 40/47 (85.11%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKD 141
            TKLYTEDWIGL+ LD+AGKVKF++V G H+ ++ +D+  Y+VPYL++
Sbjct:  253 TKLYTEDWIGLKTLDDAGKVKFVSVPGGHVRMAEEDVVKYVVPYLQN 299
[Close]
BLAST of FG539000 vs. TAIR peptide
Match: AT5G47350.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr5:19212754-19214318 FORWARD LENGTH=316)

HSP 1 Score: 66.6254 bits (161), Expect = 4.328e-12
Identity = 31/61 (50.82%), Postives = 43/61 (70.49%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKDNSYVVPYLKDQMQE 183
            TKLY EDWIGL+ L  AGKV+F+NV+GDHL ++ +D+  Y+VP L+D     P L  + +E
Sbjct:  252 TKLYIEDWIGLKALVVAGKVQFVNVTGDHLIMADEDLVKYVVPLLQDQQSAPPRLNRKTKE 312
[Close]
BLAST of FG539000 vs. TAIR peptide
Match: AT4G17483.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr4:9747375-9748892 REVERSE LENGTH=300)

HSP 1 Score: 66.2402 bits (160), Expect = 5.653e-12
Identity = 30/48 (62.50%), Postives = 37/48 (77.08%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKDN 144
            TKLY EDWIGL+ LD AGKVKF +V GDHL IS +++  ++VPYL  N
Sbjct:  251 TKLYREDWIGLKALDAAGKVKFESVLGDHLSISDEEVVEFVVPYLMQN 298
[Close]
BLAST of FG539000 vs. TAIR peptide
Match: AT4G17480.1 (| Symbols: | alpha/beta-Hydrolases superfamily protein | chr4:9745313-9746847 REVERSE LENGTH=304)

HSP 1 Score: 65.855 bits (159), Expect = 7.383e-12
Identity = 29/47 (61.70%), Postives = 39/47 (82.98%), Query Frame = 1 
Query:    1 TKLYTEDWIGLRELDEAGKVKFLNVSGDHLDISRKDMKTYIVPYLKD 141
            TKLYTEDWIGL+ LD A KVKF++V G+HL I++ D+  ++VPYLK+
Sbjct:  244 TKLYTEDWIGLKTLDAARKVKFVSVPGEHLRIAQDDVVKHVVPYLKN 290
The following BLAST results are available for this feature:
BLAST of FG539000 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
D7SLX0_VITVI5.534e-1566.67Whole genome shotgun sequence of line PN40024, sca... [more]
Q9LY31_ARATH1.610e-1479.59AT3G60340 protein OS=Arabidopsis thaliana GN=F27H5... [more]
Q93Y57_ARATH1.610e-1479.59Putative palmitoyl-protein thioesterase OS=Arabido... [more]
Q8LAH5_ARATH1.610e-1479.59Palmitoyl-protein thioesterase-like OS=Arabidopsis... [more]
A5APL1_VITVI2.747e-1464.91Putative uncharacterized protein OS=Vitis vinifera... [more]
B9GP59_POPTR6.119e-1473.47Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
D7LRP2_ARALY7.992e-1475.51Palmitoyl protein thioesterase family protein OS=A... [more]
B9RD11_RICCO1.044e-1358.44Palmitoyl-protein thioesterase 1, putative OS=Rici... [more]
Q6R749_TROMA1.780e-1358.46Putative palmitoyl-protein thioesterase OS=Tropaeo... [more]
C5WRI6_SORBI5.180e-1380.00Putative uncharacterized protein Sb01g028940 OS=So... [more]
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BLAST of FG539000 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Pisum sativum unigene v1 vs Swissprot)
Total hits: 2
Match NameE-valueIdentityDescription
PPT1_RAT1.762e-752.17Palmitoyl-protein thioesterase 1 OS=Rattus norvegi... [more]
PPT1_MOUSE5.125e-752.27Palmitoyl-protein thioesterase 1 OS=Mus musculus G... [more]
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BLAST of FG539000 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Pisum sativum unigene v1 vs TAIR 10 peptide)
Total hits: 10
Match NameE-valueIdentityDescription
AT3G60340.27.622e-1779.59| Symbols: | alpha/beta-Hydrolases superfamily pr... [more]
AT3G60340.17.622e-1779.59| Symbols: | alpha/beta-Hydrolases superfamily pr... [more]
AT4G17470.31.345e-1359.18| Symbols: | alpha/beta-Hydrolases superfamily pr... [more]
AT4G17470.21.345e-1359.18| Symbols: | alpha/beta-Hydrolases superfamily pr... [more]
AT4G17470.11.345e-1359.18| Symbols: | alpha/beta-Hydrolases superfamily pr... [more]
AT5G47330.11.943e-1259.57| Symbols: | alpha/beta-Hydrolases superfamily pr... [more]
AT5G47340.12.537e-1259.57| Symbols: | alpha/beta-Hydrolases superfamily pr... [more]
AT5G47350.14.328e-1250.82| Symbols: | alpha/beta-Hydrolases superfamily pr... [more]
AT4G17483.15.653e-1262.50| Symbols: | alpha/beta-Hydrolases superfamily pr... [more]
AT4G17480.17.383e-1261.70| Symbols: | alpha/beta-Hydrolases superfamily pr... [more]
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InterPro
Analysis Name: InterProScan analysis for Pisum sativum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002472Palmitoyl protein thioesterasePFAMPF02089Palm_thioestcoord: 2..46
score: 7.5
NoneNo IPR availableGENE3D3.40.50.1820no descriptioncoord: 1..46
score: 1
NoneNo IPR availablePANTHERPTHR11247PALMITOYL-PROTEIN THIOESTERASE/DOLICHYLDIPHOSPHATASE 1coord: 1..62
score: 7.1
NoneNo IPR availablePANTHERPTHR11247:SF8PALMITOYL-PROTEIN THIOESTERASE 1coord: 1..62
score: 7.1
NoneNo IPR availableSUPERFAMILY53474alpha/beta-Hydrolasescoord: 1..46
score: 1.6

Properties
Property NameValue
Singlet in analysisPisum sativum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>FG539000 ID=FG539000; Name=FG539000; organism=Pisum sativum; type=EST; length=383bp
ACAAAGTTGTATACTGAAGATTGGATTGGCTTAAGGGAATTAGATGAAGC
TGGGAAAGTCAAATTCTTGAACGTGTCAGGAGATCACCTGGATATCTCTC
GCAAGGACATGAAAACTTATATAGTCCCATATTTGAAGGACAATAGTTAT
GTAGTGCCATATTTGAAGGATCAAATGCAAGAATCATTCCAATCTTGAAA
AGCCATTCGGTCTGAAAAGGAATATTTCTTGCACTGACATTGATAATTAT
TGCTGATAATTGATTGTTATATTCTAATTTAGAACACACTTCTTTGAATT
TGCTTAATTTCTTAGTTTATTTCCTAAAAATACTACCCTTGTTGCATTTA
TCTTAAAAAAAAGATAAACTTTAATCTCAGAAA
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Biological Process
TermDefinition
GO:0006464cellular protein modification process
Vocabulary: Molecular Function
TermDefinition
GO:0008474palmitoyl-(protein) hydrolase activity
Vocabulary: INTERPRO
TermDefinition
IPR002472Palm_thioest