SNP-Based Linkage Mapping for Validation of QTLs for Resistance to Ascochyta Blight in Lentil

Publication Overview
TitleSNP-Based Linkage Mapping for Validation of QTLs for Resistance to Ascochyta Blight in Lentil
AuthorsSudheesh S, Rodda MS, Davidson J, Javid M, Stephens A, Slater AT, Cogan NO, Forster JW, Kaur S
TypeJournal Article
Journal NameFrontiers in plant science
Volume7
Year2016
Page(s)1604
CitationSudheesh S, Rodda MS, Davidson J, Javid M, Stephens A, Slater AT, Cogan NO, Forster JW, Kaur S. SNP-Based Linkage Mapping for Validation of QTLs for Resistance to Ascochyta Blight in Lentil. Frontiers in plant science. 2016; 7:1604.

Abstract

Lentil (Lens culinaris Medik.) is a self-pollinating, diploid, annual, cool-season, food legume crop that is cultivated throughout the world. Ascochyta blight (AB), caused by Ascochyta lentis Vassilievsky, is an economically important and widespread disease of lentil. Development of cultivars with high levels of durable resistance provides an environmentally acceptable and economically feasible method for AB control. A detailed understanding of the genetic basis of AB resistance is hence highly desirable, in order to obtain insight into the number and influence of resistance genes. Genetic linkage maps based on single nucleotide polymorphisms (SNP) and simple sequence repeat (SSR) markers have been developed from three recombinant inbred line (RIL) populations. The IH × NF map contained 460 loci across 1461.6 cM, while the IH × DIG map contained 329 loci across 1302.5 cM and the third map, NF × DIG contained 330 loci across 1914.1 cM. Data from these maps were combined with a map from a previously published study through use of bridging markers to generate a consensus linkage map containing 689 loci distributed across seven linkage groups (LGs), with a cumulative length of 2429.61 cM at an average density of one marker per 3.5 cM. Trait dissection of AB resistance was performed for the RIL populations, identifying totals of two and three quantitative trait loci (QTLs) explaining 52 and 69% of phenotypic variation for resistance to infection in the IH × DIG and IH × NF populations, respectively. Presence of common markers in the vicinity of the AB_IH1- and AB_IH2.1/AB_IH2.2-containing regions on both maps supports the inference that a common genomic region is responsible for conferring resistance and is associated with the resistant parent, Indianhead. The third QTL was derived from Northfield. Evaluation of markers associated with AB resistance across a diverse lentil germplasm panel revealed that the identity of alleles associated with AB_IH1 predicted the phenotypic responses with high levels of accuracy (~86%), and therefore have the potential to be widely adopted in lentil breeding programs. The availability of RIL-based maps, a consensus map, and validated markers linked to AB resistance provide important resources for lentil improvement.

Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
IndianheadIndianheadcultivar
NorthfieldNorthfieldcultivar
DIGGERDIGGERcultivar
Indianhead_x_NorthfieldIndianhead_x_Northfieldpopulation
Indianhead_x_DiggerIndianhead_x_Diggerpopulation
Northfield_x_DiggerNorthfield_x_Diggerpopulation
Features
This publication contains information about 686 features:
Feature NameUniquenameType
SNP_60000154SNP_60000154genetic_marker
SNP_20002815SNP_20002815genetic_marker
SNP_20003421SNP_20003421genetic_marker
SNP_20002180SNP_20002180genetic_marker
SNP_20005182SNP_20005182genetic_marker
SNP_20001125SNP_20001125genetic_marker
SNP_20001123SNP_20001123genetic_marker
PBA_LC_0323PBA_LC_0323genetic_marker
SNP_20001108SNP_20001108genetic_marker
SNP_20001124SNP_20001124genetic_marker
PBA_LC_0218PBA_LC_0218genetic_marker
SNP_20003180SNP_20003180genetic_marker
SNP_20001981SNP_20001981genetic_marker
SNP_60000178SNP_60000178genetic_marker
SNP_60000330SNP_60000330genetic_marker
PBA_LC_0412PBA_LC_0412genetic_marker
SNP_20004242SNP_20004242genetic_marker
PBA_LC_0448PBA_LC_0448genetic_marker
SNP_20000736SNP_20000736genetic_marker
SNP_20003327SNP_20003327genetic_marker
SNP_20002142SNP_20002142genetic_marker
PBA_LC_0489PBA_LC_0489genetic_marker
PBA_LC_0253PBA_LC_0253genetic_marker
SNP_20002554SNP_20002554genetic_marker
PBA_LC_0059PBA_LC_0059genetic_marker

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic-eCollection
Publication Date2016
Journal AbbreviationFront Plant Sci
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article
Journal CountrySwitzerland
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:27853461