The genome of cowpea (Vigna unguiculata [L.] Walp.)

Publication Overview
TitleThe genome of cowpea (Vigna unguiculata [L.] Walp.)
AuthorsLonardi S, Muñoz-Amatriaín M, Liang Q, Shu S, Wanamaker SI, Lo S, Tanskanen J, Schulman AH, Zhu T, Luo MC, Alhakami H, Ounit R, Hasan AM, Verdier J, Roberts PA, Santos JRP, Ndeve A, Doležel J, Vrána J, Hokin SA, Farmer AD, Cannon SB, Close TJ
TypeJournal Article
Journal NameThe Plant journal : for cell and molecular biology
Volume98
Issue5
Year2019
Page(s)767-782
CitationLonardi S, Muñoz-Amatriaín M, Liang Q, Shu S, Wanamaker SI, Lo S, Tanskanen J, Schulman AH, Zhu T, Luo MC, Alhakami H, Ounit R, Hasan AM, Verdier J, Roberts PA, Santos JRP, Ndeve A, Doležel J, Vrána J, Hokin SA, Farmer AD, Cannon SB, Close TJ. The genome of cowpea (Vigna unguiculata [L.] Walp.). The Plant journal : for cell and molecular biology. 2019 Jun; 98(5):767-782.

Abstract

Cowpea (Vigna unguiculata [L.] Walp.) is a major crop for worldwide food and nutritional security, especially in sub-Saharan Africa, that is resilient to hot and drought-prone environments. An assembly of the single-haplotype inbred genome of cowpea IT97K-499-35 was developed by exploiting the synergies between single-molecule real-time sequencing, optical and genetic mapping, and an assembly reconciliation algorithm. A total of 519 Mb is included in the assembled sequences. Nearly half of the assembled sequence is composed of repetitive elements, which are enriched within recombination-poor pericentromeric regions. A comparative analysis of these elements suggests that genome size differences between Vigna species are mainly attributable to changes in the amount of Gypsy retrotransposons. Conversely, genes are more abundant in more distal, high-recombination regions of the chromosomes; there appears to be more duplication of genes within the NBS-LRR and the SAUR-like auxin superfamilies compared with other warm-season legumes that have been sequenced. A surprising outcome is the identification of an inversion of 4.2 Mb among landraces and cultivars, which includes a gene that has been associated in other plants with interactions with the parasitic weed Striga gesnerioides. The genome sequence facilitated the identification of a putative syntelog for multiple organ gigantism in legumes. A revised numbering system has been adopted for cowpea chromosomes based on synteny with common bean (Phaseolus vulgaris). An estimate of nuclear genome size of 640.6 Mbp based on cytometry is presented.

Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint
ISSN1365-313X
eISSN1365-313X
Publication Date2019 Jun
Journal AbbreviationPlant J.
DOI10.1111/tpj.14349
Elocation10.1111/tpj.14349
Copyright© 2019 The Authors. The Plant Journal published by John Wiley & Sons Ltd and Society for Experimental Biology.
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryEngland
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:31017340