Development of an SNP-based high-density linkage map and QTL analysis for bruchid (Callosobruchus maculatus F.) resistance in black gram (Vigna mungo (L.) Hepper)

Publication Overview
TitleDevelopment of an SNP-based high-density linkage map and QTL analysis for bruchid (Callosobruchus maculatus F.) resistance in black gram (Vigna mungo (L.) Hepper)
AuthorsSomta P, Chen J, Yundaeng C, Yuan X, Yimram T, Tomooka N, Chen X
TypeJournal Article
Journal NameScientific reports
Volume9
Issue1
Year2019
Page(s)3930
CitationSomta P, Chen J, Yundaeng C, Yuan X, Yimram T, Tomooka N, Chen X. Development of an SNP-based high-density linkage map and QTL analysis for bruchid (Callosobruchus maculatus F.) resistance in black gram (Vigna mungo (L.) Hepper). Scientific reports. 2019 Mar 08; 9(1):3930.

Abstract

Black gram (Vigna mungo var. mungo) is an important pulse crop in Asia. The cowpea weevil (Callosobruchus maculatus) is a stored-seed insect pest (seed weevil/bruchid) that causes serious postharvest losses in pulse crops, including black gram. In this study, we constructed a high-density linkage map for black gram and identified quantitative trait loci (QTLs) for C. maculatus resistance. A recombinant inbred line (RIL) population of 150 lines from a cross between BC48 [cultivated black gram (var. mungo); bruchid-susceptible] and TC2210 [wild black gram (var. silvestris); bruchid-resistant] were used to construct a linkage map of 3,675 SNP markers from specific-locus amplified fragment sequencing. The map comprised 11 linkage groups spanning 1,588.7 cM with an average distance between adjacent markers of 0.57 cM. Seeds of the RIL population grown in 2016 and 2017 were evaluated for C. maculatus resistance through two traits; the percentage of damaged seeds (PDS) and infestation severity progress (AUDPS). Inclusive composite interval mapping identified three QTLs each for PDS and AUDPS. Two QTLs, qVmunBr6.1 and qVmunBr6.2, mapped about 10 cM apart on linkage group 6 were common between PDS and AUDPS. Comparative genome analysis revealed that qVmunBr6.1 and qVmunBr6.2 are new loci for C. maculatus resistance in Vigna species and that genes encoding a lectin receptor kinase and chitinase are candidates for qVmunBr6.2. The high-density linkage map constructed and QTLs for bruchid resistance identified in this study will be useful for molecular breeding of black gram.

Features
This publication contains information about 41 features:
Feature NameUniquenameType
KAM_SNP03919KAM_SNP03919genetic_marker
KAM_SNP14537KAM_SNP14537genetic_marker
KAM_SNP14536KAM_SNP14536genetic_marker
KAM_SNP14535KAM_SNP14535genetic_marker
KAM_SNP12538KAM_SNP12538genetic_marker
KAM_SNP30824KAM_SNP30824genetic_marker
KAM_SNP21560KAM_SNP21560genetic_marker
KAM_SNP19225KAM_SNP19225genetic_marker
KAM_SNP19226KAM_SNP19226genetic_marker
KAM_SNP14717KAM_SNP14717genetic_marker
KAM_SNP12165KAM_SNP12165genetic_marker
KAM_SNP12166KAM_SNP12166genetic_marker
KAM_SNP04344KAM_SNP04344genetic_marker
KAM_SNP04343KAM_SNP04343genetic_marker
KAM_SNP02358KAM_SNP02358genetic_marker
KAM_SNP17781KAM_SNP17781genetic_marker
KAM_SNP28338KAM_SNP28338genetic_marker
KAM_SNP14881KAM_SNP14881genetic_marker
KAM_SNP09422KAM_SNP09422genetic_marker
KAM_SNP09514KAM_SNP09514genetic_marker
KAM_SNP15884KAM_SNP15884genetic_marker
KAM_SNP13705KAM_SNP13705genetic_marker
KAM_SNP05044KAM_SNP05044genetic_marker
KAM_SNP16611KAM_SNP16611genetic_marker
KAM_SNP16612KAM_SNP16612genetic_marker

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Black_gram-Bruchid_resistance-Somta-2019
Featuremaps
This publication contains information about 1 maps:
Map Name
Black_gram-BC48/TC2210-RIL
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
BC48BC48accession
TC2210TC2210accession
BC48/TC2210-RILBC48/TC2210-RILpopulation
Properties
Additional details for this publication include:
Property NameValue
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryEngland
Publication ModelElectronic
ISSN2045-2322
eISSN2045-2322
Publication Date2019 Mar 08
Journal AbbreviationSci Rep
DOI10.1038/s41598-019-40669-5
Elocation10.1038/s41598-019-40669-5