Gene Mapping of a Mutant Mungbean (Vigna radiata L.) Using New Molecular Markers Suggests a Gene Encoding a YUC4-like Protein Regulates the Chasmogamous Flower Trait

Publication Overview
TitleGene Mapping of a Mutant Mungbean (Vigna radiata L.) Using New Molecular Markers Suggests a Gene Encoding a YUC4-like Protein Regulates the Chasmogamous Flower Trait
AuthorsChen J, Somta P, Chen X, Cui X, Yuan X, Srinives P
TypeJournal Article
Journal NameFrontiers in plant science
Volume7
Year2016
Page(s)830
CitationChen J, Somta P, Chen X, Cui X, Yuan X, Srinives P. Gene Mapping of a Mutant Mungbean (Vigna radiata L.) Using New Molecular Markers Suggests a Gene Encoding a YUC4-like Protein Regulates the Chasmogamous Flower Trait. Frontiers in plant science. 2016; 7:830.

Abstract

Mungbean (Vigna radiata L.) is a cleistogamous plant in which flowers are pollinated before they open, which prevents yield improvements through heterosis. We previously generated a chasmogamous mutant (CM) mungbean in which open flowers are pollinated. In this study, we developed insertion/deletion (indel) markers based on the transcriptome differences between CM and Sulu-1 (i.e., normal flowering) plants. An F2 population derived from a cross between CM and Sulu-1 was used for gene mapping. Segregation analyses revealed that a single recessive gene regulates the production of chasmogamous flowers. Using newly developed indel and simple sequence repeat markers, the cha gene responsible for the chasmogamous flower trait was mapped to a 277.1-kb segment on chromosome 6. Twelve candidate genes were detected in this segment, including Vradi06g12650, which encodes a YUCCA family protein associated with floral development. A single base pair deletion producing a frame-shift mutation and a premature stop codon in Vradi06g12650 was detected only in CM plants. This suggested that Vradi06g12650 is a cha candidate gene. Our results provide important information for the molecular breeding of chasmogamous mungbean lines, which may serve as new genetic resources for hybrid cultivar development.

Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
V1197V1197breeding_research_material
V1197-M4V1197-M4breeding_research_material
Sulu-1Sulu-1breeding_research_material
V1197M4/Sulu1-F2V1197M4/Sulu1-F2population
Features
This publication contains information about 91 features:
Feature NameUniquenameType
KU_VRID001KU_VRID001genetic_marker
KU_VRID002KU_VRID002genetic_marker
KU_VRID003KU_VRID003genetic_marker
KU_VRID004KU_VRID004genetic_marker
KU_VRID007KU_VRID007genetic_marker
KU_VRID008KU_VRID008genetic_marker
KU_VRID009KU_VRID009genetic_marker
KU_VRID012KU_VRID012genetic_marker
KU_VRID013KU_VRID013genetic_marker
KU_VRID014KU_VRID014genetic_marker
KU_VRID019KU_VRID019genetic_marker
KU_VRID022KU_VRID022genetic_marker
KU_VRID023KU_VRID023genetic_marker
KU_VRID025KU_VRID025genetic_marker
KU_VRID026KU_VRID026genetic_marker
KU_VRID028KU_VRID028genetic_marker
KU_VRID029KU_VRID029genetic_marker
KU_VRID031KU_VRID031genetic_marker
KU_VRID032KU_VRID032genetic_marker
KU_VRID034KU_VRID034genetic_marker
KU_VRID035KU_VRID035genetic_marker
KU_VRID036KU_VRID036genetic_marker
KU_VRID040KU_VRID040genetic_marker
KU_VRID041KU_VRID041genetic_marker
KU_VRID043KU_VRID043genetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
Vigna-V1197M4/Sulu1-F2
Properties
Additional details for this publication include:
Property NameValue
Publication Date2016
Journal AbbreviationFront Plant Sci
DOI10.3389/fpls.2016.00830
Elocation10.3389/fpls.2016.00830
Publication ModelElectronic-eCollection
ISSN1664-462X
pISSN1664-462X
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountrySwitzerland
Cross References
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DatabaseAccession
PMID: PubMedPMID:27375671