A major QTL corresponding to the Rk locus for resistance to root-knot nematodes in cowpea (Vigna unguiculata L. Walp.)

Publication Overview
TitleA major QTL corresponding to the Rk locus for resistance to root-knot nematodes in cowpea (Vigna unguiculata L. Walp.)
AuthorsHuynh BL, Matthews WC, Ehlers JD, Lucas MR, Santos JR, Ndeve A, Close TJ, Roberts PA
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Volume129
Issue1
Year2016
Page(s)87-95
CitationHuynh BL, Matthews WC, Ehlers JD, Lucas MR, Santos JR, Ndeve A, Close TJ, Roberts PA. A major QTL corresponding to the Rk locus for resistance to root-knot nematodes in cowpea (Vigna unguiculata L. Walp.). TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 2016 Jan; 129(1):87-95.

Abstract

KEY MESSAGE
Genome resolution of a major QTL associated with the Rk locus in cowpea for resistance to root-knot nematodes has significance for plant breeding programs and R gene characterization. Cowpea (Vigna unguiculata L. Walp.) is a susceptible host of root-knot nematodes (Meloidogyne spp.) (RKN), major plant-parasitic pests in global agriculture. To date, breeding for host resistance in cowpea has relied on phenotypic selection which requires time-consuming and expensive controlled infection assays. To facilitate marker-based selection, we aimed to identify and map quantitative trait loci (QTL) conferring the resistance trait. One recombinant inbred line (RIL) and two F2:3 populations, each derived from a cross between a susceptible and a resistant parent, were genotyped with genome-wide single nucleotide polymorphism (SNP) markers. The populations were screened in the field for root-galling symptoms and/or under growth-chamber conditions for nematode reproduction levels using M. incognita and M. javanica biotypes. One major QTL was mapped consistently on linkage group VuLG11 of each population. By genotyping additional cowpea lines and near-isogenic lines derived from conventional backcrossing, we confirmed that the detected QTL co-localized with the genome region associated with the Rk locus for RKN resistance that has been used in conventional breeding for many decades. This chromosomal location defined with flanking markers will be a valuable target in marker-assisted breeding and for positional cloning of genes controlling RKN resistance.

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Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Cowpea-Nematode_resistance-Huynh-2015
Featuremaps
This publication contains information about 1 maps:
Map Name
Cowpea-UCR_consensus_map-2015
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
CB-27/24-125B-1-RILCB-27/24-125B-1-RILpopulation
IT84S-2049/UCR-779-F2:3IT84S-2049/UCR-779-F2:3population
IT93K-503-1/UCR-779-F2:3IT93K-503-1/UCR-779-F2:3population
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1432-2242
eISSN1432-2242
Publication Date2016 Jan
Journal AbbreviationTheor. Appl. Genet.
DOI10.1007/s00122-015-2611-0
Elocation10.1007/s00122-015-2611-0
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryGermany
Publication TypeResearch Support, Non-U.S. Gov't
Publication TypeResearch Support, U.S. Gov't, Non-P.H.S.