BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features

Publication Overview
TitleBAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features
AuthorsBlair MW, Córdoba JM, Muñóz C, Yuyó DK
TypeJournal Article
Journal NamePloS one
Volume9
Issue9
Year2014
Page(s)e101873
CitationBlair MW, Córdoba JM, Muñóz C, Yuyó DK. BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features. PloS one. 2014; 9(9):e101873.

Abstract

Highly polymorphic markers such as simple sequence repeats (SSRs) or microsatellites are very useful for genetic mapping. In this study novel SSRs were identified in BAC-end sequences (BES) from non-contigged, non-overlapping bacterial artificial clones (BACs) in common bean (Phaseolus vulgaris L.). These so called "singleton" BACs were from the G19833 Andean gene pool physical map and the new BES-SSR markers were used for the saturation of the inter-gene pool, DOR364×G19833 genetic map. A total of 899 SSR loci were found among the singleton BES, but only 346 loci corresponded to the single di- or tri-nucleotide motifs that were likely to be polymorphic (ATT or AG motifs, principally) and useful for primer design and individual marker mapping. When these novel SSR markers were evaluated in the DOR364×G19833 population parents, 136 markers revealed polymorphism and 106 were mapped. Genetic mapping resulted in a map length of 2291 cM with an average distance between markers of 5.2 cM. The new genetic map was compared to the most recent cytogenetic analysis of common bean chromosomes. We found that the new singleton BES-SSR were helpful in filling peri-centromeric spaces on the cytogenetic map. Short genetic distances between some new singleton-derived BES-SSR markers was common showing suppressed recombination in these regions compared to other parts of the genome. The correlation of singleton-derived SSR marker distribution with other cytogenetic features of the bean genome is discussed.

Features
This publication contains information about 507 features:
Feature NameUniquenameType
BMb-0002BMb-0002genetic_marker
BMb-0010BMb-0010genetic_marker
BMb-0032BMb-0032genetic_marker
BMb-0064BMb-0064genetic_marker
BMb-0066BMb-0066genetic_marker
BMb-0080BMb-0080genetic_marker
BMb-0083BMb-0083genetic_marker
BMb-0096BMb-0096genetic_marker
BMb-0097BMb-0097genetic_marker
BMb-0106BMb-0106genetic_marker
BMb-0121BMb-0121genetic_marker
BMb-0125BMb-0125genetic_marker
BMb-0133BMb-0133genetic_marker
BMb-0143BMb-0143genetic_marker
BMb-0152BMb-0152genetic_marker
BMb-0160BMb-0160genetic_marker
BMb-0174BMb-0174genetic_marker
BMb-0180BMb-0180genetic_marker
BMb-0182BMb-0182genetic_marker
BMb-0185BMb-0185genetic_marker
BMb-0191BMb-0191genetic_marker
BMb-0194BMb-0194genetic_marker
BMb-0202BMb-0202genetic_marker
BMb-0213BMb-0213genetic_marker
BMb-0247BMb-0247genetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
Bean-DOR364/G19833-RIL
Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic-eCollection
ISSN1932-6203
eISSN1932-6203
Publication Date2014
Journal AbbreviationPLoS ONE
DOI10.1371/journal.pone.0101873
Elocation10.1371/journal.pone.0101873
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryUnited States
Publication TypeResearch Support, Non-U.S. Gov't